CN103766328A - Manufacturing method for plant leaf dipping specimen - Google Patents
Manufacturing method for plant leaf dipping specimen Download PDFInfo
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- CN103766328A CN103766328A CN201410015946.5A CN201410015946A CN103766328A CN 103766328 A CN103766328 A CN 103766328A CN 201410015946 A CN201410015946 A CN 201410015946A CN 103766328 A CN103766328 A CN 103766328A
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- leaf blade
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- formalin
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Abstract
The invention provides a manufacturing method for a plant leaf dipping specimen and particularly relates to a manufacturing method for a tobacco brown spot leaf dipping specimen. The manufacturing method comprises the following steps: putting a tobacco brown spot leaf specimen into a copper acetate crystal and an acetic acid mixed diluting solution to be boiled by water; and then putting the specimen into a mixed solution composed of formalin, zinc sulfate and cloves oil to be preserved. The manufacturing method for the specimen is simple and the preservation time of the manufactured specimen is low long; and the problem that the leaf specimen is faded in the preservation process does not happen.
Description
Technical field
The invention belongs to preparation of specimen field, relate in particular to a kind of preparation method of plant leaf blade dipping specimen.
Background technology
The store method of existing Alternaria alternate leaf blade specimen is more, wherein, comprises that Alternaria alternate leaf blade specimen is placed in Schweinfurt green crystallization by employing and acetic acid mixed diluting liquid poach is placed on the method that formalin is preserved.But the sample holding time of the method is limited, generally just there will be the phenomenon of fading at 1 year left and right blade of preservation, this method cannot be used for the long-term preservation of blade.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of plant leaf blade dipping specimen, be intended to solve the existing plant leaf blade holding time more long afterwards leaf blade specimen there is the problem of fading.
The present invention is achieved in that a kind of preparation method of plant leaf blade dipping specimen, comprises the following steps:
(1) Schweinfurt green crystallization is added in gradually in 50% acetum, in the time that Schweinfurt green crystallization is no longer dissolved, stops adding Schweinfurt green crystallization, obtain stoste, 3～4 times of described stoste dilute with waters are obtained to dilution;
(2) plant leaf blade specimen is placed in the dilution that the step (1) after boiling obtains, sample color is taken off gradually, continue heating 3～4min, after sample color restoration, sample is taken out and is stored in the mixed liquor being made up of formalin, zinc sulphate and cloves oil, wherein, described formalin, zinc sulphate and the cloves oil concentration expressed in percentage by volume in mixed solution is respectively (4～6) %, (2～3) % and (0.05～0.15) %.
Preferably, described plant leaf blade is Alternaria alternate blade.
Preferably, in step (2), described formalin, zinc sulphate and the cloves oil concentration expressed in percentage by volume in mixed solution is respectively 5%, 2.5% and 0.1%.
The present invention overcomes the deficiencies in the prior art, a kind of preparation method of plant leaf blade dipping specimen is provided, adopt Alternaria alternate leaf blade specimen is placed in after the crystallization of Schweinfurt green knot and acetic acid mixed diluting liquid poach, then the mixed solution that is placed in formalin, zinc sulphate and cloves oil composition is preserved.In the present invention, between formalin, zinc sulphate and cloves oil, can coordinated play stabilizer vane sample internal organizational structure and character, and percentage relation between formalin, zinc sulphate and cloves oil also plays very important effect for the stable preservation of leaf blade specimen.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1
A preparation method for plant leaf blade dipping specimen, comprises the following steps:
(1) Schweinfurt green crystallization is added in gradually in 50% acetum, in the time that Schweinfurt green crystallization is no longer dissolved, stops adding Schweinfurt green crystallization, obtain stoste, 3 times of described stoste dilute with waters are obtained to dilution;
(2) chrysanthemum leaf sample is placed in the dilution that the step (1) after boiling obtains, sample color is taken off gradually, continue heating 4min, after sample being taken out after sample color restoration, in the mixed liquor that the formalin that to be stored in by concentration expressed in percentage by volume be 4%, 3% zinc sulphate and 0.05% cloves oil form.
Embodiment 2
A preparation method for plant leaf blade dipping specimen, comprises the following steps:
(1) Schweinfurt green crystallization is added in gradually in 50% acetum, in the time that Schweinfurt green crystallization is no longer dissolved, stops adding Schweinfurt green crystallization, obtain stoste, 3～4 times of described stoste dilute with waters are obtained to dilution;
(2) lotus leaf blade specimen is placed in the dilution that the step (1) after boiling obtains, sample color is taken off gradually, continue heating 4min, after sample being taken out after sample color restoration, in the mixed liquor that the formalin that to be stored in by concentration expressed in percentage by volume be 6%, 2% zinc sulphate and 0.05% cloves oil form.
Embodiment 3
A preparation method for plant leaf blade dipping specimen, comprises the following steps:
(1) Schweinfurt green crystallization is added in gradually in 50% acetum, in the time that Schweinfurt green crystallization is no longer dissolved, stops adding Schweinfurt green crystallization, obtain stoste, 3～4 times of described stoste dilute with waters are obtained to dilution;
(2) Alternaria alternate leaf blade specimen is placed in the dilution that the step (1) after boiling obtains, sample color is taken off gradually, continue heating 3min, after sample being taken out after sample color restoration, in the mixed liquor that the formalin that to be stored in by concentration expressed in percentage by volume be 4%, 3% zinc sulphate and 0.05% cloves oil form.
Embodiment 4
A preparation method for plant leaf blade dipping specimen, comprises the following steps:
(1) Schweinfurt green crystallization is added in gradually in 50% acetum, in the time that Schweinfurt green crystallization is no longer dissolved, stops adding Schweinfurt green crystallization, obtain stoste, 3～4 times of described stoste dilute with waters are obtained to dilution;
(2) Alternaria alternate leaf blade specimen is placed in the dilution that the step (1) after boiling obtains, sample color is taken off gradually, continue heating 4min, after sample being taken out after sample color restoration, in the mixed liquor that the formalin that to be stored in by concentration expressed in percentage by volume be 6%, 2% zinc sulphate and 0.15% cloves oil form.
Embodiment 5
A preparation method for plant leaf blade dipping specimen, comprises the following steps:
(1) Schweinfurt green crystallization is added in gradually in 50% acetum, in the time that Schweinfurt green crystallization is no longer dissolved, stops adding Schweinfurt green crystallization, obtain stoste, 3～4 times of described stoste dilute with waters are obtained to dilution;
(2) Alternaria alternate leaf blade specimen is placed in the dilution that the step (1) after boiling obtains, sample color is taken off gradually, continue heating 3～4min, after sample being taken out after sample color restoration, in the mixed liquor that the formalin that to be stored in by concentration expressed in percentage by volume be 5%, 2.5% zinc sulphate and 0.1% cloves oil form.
Comparative example
A preparation method for plant leaf blade dipping specimen, comprises the following steps:
(1) Schweinfurt green crystallization is added in gradually in 50% acetum, in the time that Schweinfurt green crystallization is no longer dissolved, stops adding Schweinfurt green crystallization, obtain stoste, 3～4 times of described stoste dilute with waters are obtained to dilution;
(2) Alternaria alternate leaf blade specimen is placed in the dilution that the step (1) after boiling obtains, sample color is taken off gradually, continues heating 3～4min, after after sample color restoration, sample being taken out, in the formalin that to be stored in by concentration expressed in percentage by volume be 5%.
Effect embodiment
Leaf blade specimen prepared in leaf blade specimen prepared in embodiment 1～5 and comparative example is stored under equivalent environment, wherein, leaf blade specimen in comparative example starts to fade at 1 year left and right blade of storage, and the Leaf sample color of the embodiment of the present invention 1～5 does not change.
Compare and the shortcoming and defect of prior art, the present invention has following beneficial effect: leaf blade specimen preparation method of the present invention is simple, and the sample holding time of made is longer, and the problem that there will not be leaf blade specimen to fade in preservation process.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (3)
1. a preparation method for plant leaf blade dipping specimen, is characterized in that comprising the following steps:
(1) Schweinfurt green crystallization is added in gradually in 50% acetum, in the time that Schweinfurt green crystallization is no longer dissolved, stops adding Schweinfurt green crystallization, obtain stoste, 3～4 times of described stoste dilute with waters are obtained to dilution;
(2) plant leaf blade specimen is placed in the dilution that the step (1) after boiling obtains, sample color is taken off gradually, continue heating 3～4min, after sample color restoration, sample is taken out and is stored in the mixed liquor being made up of formalin, zinc sulphate and cloves oil, wherein, described formalin, zinc sulphate and the cloves oil concentration expressed in percentage by volume in mixed solution is respectively (4～6) %, (2～3) % and (0.05～0.15) %.
2. the preparation method of plant leaf blade dipping specimen as claimed in claim 1, is characterized in that, described plant leaf blade is Alternaria alternate blade.
3. the preparation method of plant leaf blade dipping specimen as claimed in claim 2, is characterized in that, in step (2), described formalin, zinc sulphate and the cloves oil concentration expressed in percentage by volume in mixed solution is respectively 5%, 2.5% and 0.1%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410015946.5A CN103766328B (en) | 2014-01-14 | 2014-01-14 | A kind of preparation method of plant leaf blade dipping specimen |
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| CN201410015946.5A CN103766328B (en) | 2014-01-14 | 2014-01-14 | A kind of preparation method of plant leaf blade dipping specimen |
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| CN103766328A true CN103766328A (en) | 2014-05-07 |
| CN103766328B CN103766328B (en) | 2015-12-30 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108770840A (en) * | 2018-08-09 | 2018-11-09 | 四川省农业科学院植物保护研究所 | A kind of tobacco diseases leaf blade specimen dipping preserves liquid and Slide processing |
| CN109085028A (en) * | 2018-06-07 | 2018-12-25 | 西南大学 | The production method that citrus leaves disease solidifies sample |
| CN109169639A (en) * | 2018-08-09 | 2019-01-11 | 四川省农业科学院植物保护研究所 | A kind of tobacco rhizome disease dipping specimen saves liquid and Slide processing |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1273775A (en) * | 1999-05-13 | 2000-11-22 | 徐永安 | Deodouring and antistaling agent for storing animal or plant bodies |
| CN103027033A (en) * | 2011-10-08 | 2013-04-10 | 云南大学中草药生物资源研究所 | Cordyceps specimen preservation solution |
-
2014
- 2014-01-14 CN CN201410015946.5A patent/CN103766328B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1273775A (en) * | 1999-05-13 | 2000-11-22 | 徐永安 | Deodouring and antistaling agent for storing animal or plant bodies |
| CN103027033A (en) * | 2011-10-08 | 2013-04-10 | 云南大学中草药生物资源研究所 | Cordyceps specimen preservation solution |
Non-Patent Citations (1)
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| 刘淑琴: "原形原色生物标本的制作及保存方法", 《教学仪器与实验》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109085028A (en) * | 2018-06-07 | 2018-12-25 | 西南大学 | The production method that citrus leaves disease solidifies sample |
| CN108770840A (en) * | 2018-08-09 | 2018-11-09 | 四川省农业科学院植物保护研究所 | A kind of tobacco diseases leaf blade specimen dipping preserves liquid and Slide processing |
| CN109169639A (en) * | 2018-08-09 | 2019-01-11 | 四川省农业科学院植物保护研究所 | A kind of tobacco rhizome disease dipping specimen saves liquid and Slide processing |
| CN108770840B (en) * | 2018-08-09 | 2021-01-19 | 四川省农业科学院植物保护研究所 | Tobacco disease leaf specimen dipping preservation liquid and specimen preparation method |
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| Publication number | Publication date |
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| CN103766328B (en) | 2015-12-30 |
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