CN104872269A - Red shrimp preservative and preparation method thereof - Google Patents
Red shrimp preservative and preparation method thereof Download PDFInfo
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- CN104872269A CN104872269A CN201410824273.8A CN201410824273A CN104872269A CN 104872269 A CN104872269 A CN 104872269A CN 201410824273 A CN201410824273 A CN 201410824273A CN 104872269 A CN104872269 A CN 104872269A
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- 241000238562 Farfantepenaeus aztecus Species 0.000 title claims abstract description 60
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000003755 preservative agent Substances 0.000 title abstract 5
- 230000002335 preservative effect Effects 0.000 title abstract 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 42
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims abstract description 25
- DOUMFZQKYFQNTF-WUTVXBCWSA-N (R)-rosmarinic acid Chemical compound C([C@H](C(=O)O)OC(=O)\C=C\C=1C=C(O)C(O)=CC=1)C1=CC=C(O)C(O)=C1 DOUMFZQKYFQNTF-WUTVXBCWSA-N 0.000 claims abstract description 24
- DOUMFZQKYFQNTF-MRXNPFEDSA-N rosemarinic acid Natural products C([C@H](C(=O)O)OC(=O)C=CC=1C=C(O)C(O)=CC=1)C1=CC=C(O)C(O)=C1 DOUMFZQKYFQNTF-MRXNPFEDSA-N 0.000 claims abstract description 14
- DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 claims abstract description 13
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 claims abstract description 13
- 108010015776 Glucose oxidase Proteins 0.000 claims abstract description 13
- 239000004366 Glucose oxidase Substances 0.000 claims abstract description 13
- 102000003792 Metallothionein Human genes 0.000 claims abstract description 13
- 108090000157 Metallothionein Proteins 0.000 claims abstract description 13
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 claims abstract description 13
- 108010053775 Nisin Proteins 0.000 claims abstract description 13
- 229940116332 glucose oxidase Drugs 0.000 claims abstract description 13
- 235000019420 glucose oxidase Nutrition 0.000 claims abstract description 13
- 239000004309 nisin Substances 0.000 claims abstract description 13
- 235000010297 nisin Nutrition 0.000 claims abstract description 13
- ZZAFFYPNLYCDEP-HNNXBMFYSA-N Rosmarinsaeure Natural products OC(=O)[C@H](Cc1cccc(O)c1O)OC(=O)C=Cc2ccc(O)c(O)c2 ZZAFFYPNLYCDEP-HNNXBMFYSA-N 0.000 claims abstract description 12
- TVHVQJFBWRLYOD-UHFFFAOYSA-N rosmarinic acid Natural products OC(=O)C(Cc1ccc(O)c(O)c1)OC(=Cc2ccc(O)c(O)c2)C=O TVHVQJFBWRLYOD-UHFFFAOYSA-N 0.000 claims abstract description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 8
- 239000008103 glucose Substances 0.000 claims abstract description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 32
- 239000007864 aqueous solution Substances 0.000 claims description 25
- 229910004298 SiO 2 Inorganic materials 0.000 claims description 22
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 14
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 11
- 239000005018 casein Substances 0.000 claims description 11
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 11
- 235000021240 caseins Nutrition 0.000 claims description 11
- 229910052708 sodium Inorganic materials 0.000 claims description 11
- 239000011734 sodium Substances 0.000 claims description 11
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- 230000000694 effects Effects 0.000 abstract description 15
- 229920001661 Chitosan Polymers 0.000 abstract description 5
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- 102000011632 Caseins Human genes 0.000 abstract 1
- 108010076119 Caseins Proteins 0.000 abstract 1
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- 229910052681 coesite Inorganic materials 0.000 abstract 1
- 229910052906 cristobalite Inorganic materials 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 239000000377 silicon dioxide Substances 0.000 abstract 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 1
- 229940080237 sodium caseinate Drugs 0.000 abstract 1
- 229910052682 stishovite Inorganic materials 0.000 abstract 1
- 229910052905 tridymite Inorganic materials 0.000 abstract 1
- 241000238557 Decapoda Species 0.000 description 12
- 229960000583 acetic acid Drugs 0.000 description 12
- 238000004321 preservation Methods 0.000 description 12
- 102000003425 Tyrosinase Human genes 0.000 description 4
- 108060008724 Tyrosinase Proteins 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- SSISHJJTAXXQAX-ZETCQYMHSA-N L-ergothioneine Chemical compound C[N+](C)(C)[C@H](C([O-])=O)CC1=CNC(=S)N1 SSISHJJTAXXQAX-ZETCQYMHSA-N 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 239000012362 glacial acetic acid Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- MBLBDJOUHNCFQT-LXGUWJNJSA-N aldehydo-N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
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- DBTMGCOVALSLOR-DEVYUCJPSA-N (2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)O[C@H](O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-DEVYUCJPSA-N 0.000 description 1
- 229930185605 Bisphenol Natural products 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical group [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001235208 Farfantepenaeus paulensis Species 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 229920001543 Laminarin Polymers 0.000 description 1
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- 241000727771 Solenocera melantho Species 0.000 description 1
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- IISBACLAFKSPIT-UHFFFAOYSA-N bisphenol A Chemical compound C=1C=C(O)C=CC=1C(C)(C)C1=CC=C(O)C=C1 IISBACLAFKSPIT-UHFFFAOYSA-N 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- 229940088598 enzyme Drugs 0.000 description 1
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- 239000003205 fragrance Substances 0.000 description 1
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- 150000002632 lipids Chemical class 0.000 description 1
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- 235000013372 meat Nutrition 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
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- 239000000049 pigment Substances 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a red shrimp preservative which is composed of the following ingredients, by weight, 0.01-0.03% of glucose oxidase, 1-3% of glucose, 0.3-0.5% of dithiothreitol, 0.03-0.05% of nisin, 0.5-1% of sodium caseinate, 0.01-0.05% of modified nano-SiO2, 0.02-0.05% of monoglyceride, 1-2% of chitosan, 0.05-0.1% of metallothionein, 3-5% of rosmarinic acid and the rest being 0.5-1% of an aqueous acetic acid solution. The red shrimp preservative provided by the invention has a scientific and reasonable formula, is low-cost, has excellent bacteriostatic, fresh-keeping, color-protecting and anti-blackening effects, can simultaneously inhibit color-fading, blackening, spoilage and carapace detachment of red shrimp, and can prolong freshness lifetime of red shrimp. By the use of the red shrimp preservative, quality of red shrimp is good. The invention also discloses a preparation method of the red shrimp preservative. The preparation method has advantages of simple preparation step, low investment in equipment and low cost, and is suitable for large-scale industrial production.
Description
Technical field
The present invention relates to aquatic products technical field of preservation of fresh, especially relate to a kind of red shrimp antistaling agent and preparation method thereof.
Background technology
Red shrimp, Latin is called Solenocera Melantho, and being distributed widely in China's the East China Sea and the Yellow Sea south and the South Sea, is the Dominant variety of Offshore of Zhejiang Province resource, especially higher in the central and north, Zhejiang output.Because it has natural pink shrimp shell, the soft cunning of fine and tender taste, fresh fragrance are beautiful, and are rich in protein and multiple nutrients material, there is very high food therapy value, the immunity of human body can be strengthened, no matter be therefore outward appearance or meat, be obtained for the high praise energetically of consumers in general.
Treat after red shrimp catches that the time that fishing boat returns port generally needs 3 ~ 5d, add that the catching season of red shrimp is summer and autumn, temperature is higher, and red shrimp moisture is high, therefore in transportation red shrimp Ji Yi ?become rot, for slow down red shrimp Tui Se ?to become and rotten, at present at sea the most frequently used preservation method to be cased preservation on the rocks (i.e. ice preservation) to extra large shrimping in transportation, ice preservation cost is low, the needs of suitable mass transit, but still there is following deficiency in ice preservation: (1) fresh keeping time is short, maximum duration is 5 days, and easily cause shrimp body dewater and cause quality to decline, (2) carapace of red shrimp is comparatively large, and ice preservation easily causes the carapace of red shrimp to come off, and affects exterior quality, (3) cause shrimp shell to fade to turn white, exterior quality reduces greatly.Therefore, the fresh keeping time and the fresh-keeping effect that improve red shrimp ice preservation just seem particularly important.
Application publication number CN103948139A, Shen Qing Publication day 2014.07.30, name is called that a kind of the suppression discloses a kind of antistaling agent suppressing the black change of prawn in the antistaling agent of the black change of prawn and the Chinese patent of preservation method thereof, this antistaling agent, this antistaling agent is composed of the following components by mass percentage: the erythrothioneine of 0.2% ~ 0.9%, the sea-tangle polyphenol of 0.3% ~ 0.9%, the laminarin of 0.3% ~ 1%, the glacial acetic acid of 0.6% ~ 1%, surplus is water.This antistaling agent has the following disadvantages: erythrothioneine has antioxidation, its anti-black change principle is: erythrothioneine can restraint of tyrosinase catalysis leuco-compounds list phenol, himself can be used as substrate, to have precedence over leuco-compounds list phenol oxidized, thus reach minimizing substrate is produced atrament amount by tyrosinase oxidation, suppress the object of the black change of prawn, but it does not inherently suppress the activity of tyrosine, make shrimp body still can exist to a certain degree ?become, result of use is not good enough; Secondly, be added with glacial acetic acid in this antistaling agent, glacial acetic acid add the local flavor that membership affects prawn; In addition, the component cost in this antistaling agent is high, should not promote; Effectively can not suppress fading of red shrimp and coming off of carapace.
Summary of the invention
The present invention is that the Preserving Reagent Freshening Effect in order to solve prior art is poor, act on single, effectively can not suppress the fading of red shrimp, dewater and the problem come off of carapace, provide a kind of scientific formulation reasonable, cost is low, can suppress simultaneously red shrimp fade, ?become, putrid and deteriorated and carapace comes off, and can extend red shrimp fresh keeping time, the red shrimp antistaling agent of good refreshing effect.
The invention also discloses a kind of preparation method of red shrimp antistaling agent, preparation process is simple, and equipment investment is few, and cost is low, is applicable to large-scale industrial production.
To achieve these goals, the present invention is by the following technical solutions:
A kind of red shrimp antistaling agent, is made up of the component of following mass percent: 0.01 ~ 0.03% glucose oxidase, 1 ~ 3% glucose, 0.3 ~ 0.5% dithiothreitol (DTT), 0.03 ~ 0.05% nisin, 0.5 ~ 1% casein sodium, 0.01 ~ 0.05% modified Nano SiO
2, 0.02 ~ 0.05% monoglyceride, 1 ~ 2% shitosan, 0.05 ~ 0.1% metallothionein, 3 ~ 5% Rosmarinic acids, surplus to be mass percent concentration be 0.5 ~ 1% acetic acid aqueous solution.Antistaling agent of the present invention is a kind of coating antistaling agent; scientific formulation is reasonable; each component is easy to get, and cost is low, and wherein film forming agent is shitosan; but chitosan solution is not easily evenly attached to red shrimp body surface and forms the homogeneous diaphragm of thickness; film forming is poor, and after film forming, permeability rate is unstable, and resistance oxygen water preventing ability, obdurability and moisture retention are poor; affect fresh-keeping effect, therefore add modified Nano SiO in the present invention
2solve the problem with monoglyceride, modified Nano SiO
2good dispersion, can film forming be improved, and after film forming without crack performance produce, also can improve anti-microbial property and the mechanical performance of chitosan film, and significantly can improve the resistance oxygen block-water performance of chitosan film; Monoglyceride can improve modified Nano SiO
2and the dispersing uniformity of other components, and can mobility of the present invention be improved, make chitosan solution evenly can be attached to red shrimp body surface and form the uniform diaphragm of thickness, the water-retaining property of film can also be improved further, and the transparency of film can be improved, be conducive to the exterior quality improving red shrimp; Glucose oxidase plays deoxidation, and the oxygen under the effect of glucose oxidase around red shrimp can react with glucose and remove, be conducive to suppressing Xia Ti ?become, glucose also can maintain color and the gloss of red shrimp, is conducive to improving exterior quality; The metal ion (copper core) of the rapid chelating tyrosinase activity point position of dithiothreitol (DTT) energy, reaches the object suppressing enzymatic ?to become; Nisin has very excellent biocidal property, and security is good, can extend the freshness date of red shrimp; Rosmarinic acid can suppress the activity of various metabolic enzyme in shrimp body, delay the putrid and deteriorated of red shrimp, in addition, Rosmarinic acid also has bacteriostasis, and with nisin coordinated, fungistatic effect improves greatly, the most important thing is, Rosmarinic acid has effect that is anti-oxidant, fixation, can effectively prevent the oxidation of pigment in red shrimp and fade, and is conducive to the organoleptic quality improving red shrimp; Casein sodium can improve retentiveness and the stability of shrimp body, the components such as the lipid in shrimp, moisture and musculature can also be combined securely, form compact texture, to reduce the loss of moisture, nutriment and flavor substance in preserving process, efficiently solve red shrimp in the problem that ice preservation process Prawn body easily dewaters and carapace comes off and causes quality to decline; Metallothionein has very strong demetalization ability, especially have very strong selective to copper ion, with dithiothreitol (DTT) coordinated, the activity of the thorough restraint of tyrosinase of energy, thus thoroughly suppress black change, in addition metallothionein can reduce the accumulation of oxygen in shrimp body, impels bis-phenol to be transformed into colourless compound, thus suppress further Hong Xia ?become; Acetic acid aqueous solution is as solvent, to ensure that each component can fast and dissolve completely.The mutual Synergistic of each component in antistaling agent of the present invention, have excellent antibacterial, fresh-keeping, protect look, anti-black change effect, can suppress simultaneously red shrimp fade, ?become, putrid and deteriorated and carapace comes off, and can extend red shrimp fresh keeping time, and the quality better of red shrimp, have broad application prospects.
As preferably, described modification SiO
2obtain by the following method: by Nano-meter SiO_2
2adding mass percent concentration is in the sodium dodecyl sulfate aqueous solution of 0.5 ~ 0.7%, Nano-meter SiO_2
2be 1:100 ~ 150 with the mass ratio of sodium dodecyl sulfate aqueous solution, regulate pH to be after 4 ~ 5, stir 6 ~ 8h, filter, wash with water, finally by dry, grind and obtain modified Nano SiO
2.To Nano-meter SiO_2
2carry out modification and obtain modified Nano SiO
2, make Nano-meter SiO_2
2there is good dispersiveness, not easily reunite.
A preparation method for red shrimp antistaling agent, concrete steps are:
(1) modified Nano SiO is prepared
2: by Nano-meter SiO_2
2adding mass percent concentration is in the sodium dodecyl sulfate aqueous solution of 0.5 ~ 0.7%, Nano-meter SiO_2
2be 1:100 ~ 150 with the mass ratio of sodium dodecyl sulfate aqueous solution, regulate pH to be after 4 ~ 5, stir 6 ~ 8h, filter, wash with water, finally by dry, grind and obtain modified Nano SiO
2, stand-by.
(2) mixture: after taking each component respectively by above-mentioned mass percent, by modified Nano SiO
2add in acetic acid aqueous solution, after ultrasonic disperse is even, add shitosan, add monoglyceride after abundant dissolving, finally add glucose oxidase, glucose, dithiothreitol (DTT), nisin, casein sodium, metallothionein and Rosmarinic acid stir after degassed 10 ~ 15min under vacuum.The mode mixed by Multistep feeding can obtain antistaling agent of the present invention, and preparation process is simple, and equipment investment is few, and cost is low, is applicable to large-scale industrial production.
Therefore, the present invention has following beneficial effect:
(1) devise for the biochemical characteristic of red shrimp and be a kind ofly exclusively used in the fresh-keeping antistaling agent of red shrimp, scientific formulation is reasonable, cost is low, by the mutual Synergistic of each component, have excellent antibacterial, fresh-keeping, protect look, anti-black change effect, can suppress simultaneously red shrimp fade, ?become, putrid and deteriorated and carapace comes off, and can extend red shrimp fresh keeping time, and the quality better of red shrimp, have broad application prospects;
(2) mode mixed by Multistep feeding can obtain antistaling agent of the present invention, and preparation process is simple, and equipment investment is few, and cost is low, is applicable to large-scale industrial production.
Detailed description of the invention
Below by detailed description of the invention, the present invention will be further described.
In the present invention, if not refer in particular to, all percentage is unit of weight, and all devices and raw material all can be buied from market or the industry is conventional, and the method in following embodiment, if no special instructions, is this area conventional method.
Embodiment 1
A kind of red shrimp antistaling agent, is made up of the component of following mass percent: 0.01% glucose oxidase, 1% glucose, 0.3% dithiothreitol (DTT), 0.03% nisin, 0.5% casein sodium, 0.01% modified Nano SiO
2, 0.02% monoglyceride, 1% shitosan, 0.05% metallothionein, 3% Rosmarinic acid, surplus to be mass percent concentration be 0.5% acetic acid aqueous solution.
This red shrimp antistaling agent obtains by the following method:
(1) modified Nano SiO is prepared
2: by Nano-meter SiO_2
2adding mass percent concentration is in the sodium dodecyl sulfate aqueous solution of 0.5%, Nano-meter SiO_2
2be 1:100 with the mass ratio of sodium dodecyl sulfate aqueous solution, regulate pH to be after 4, stir 6h, filter, wash with water, finally by dry, grind and obtain modified Nano SiO
2, stand-by;
(2) mixture: after taking each component respectively by above-mentioned mass percent, by modified Nano SiO
2add in acetic acid aqueous solution, after ultrasonic disperse is even, add shitosan, add monoglyceride after abundant dissolving, finally add glucose oxidase, glucose, dithiothreitol (DTT), nisin, casein sodium, metallothionein and Rosmarinic acid stir after degassed 10 ~ min under vacuum.
Embodiment 2
A kind of red shrimp antistaling agent, is made up of the component of following mass percent: 0.02% glucose oxidase, 2% glucose, 0.4% dithiothreitol (DTT), 0.04% nisin, 0.8% casein sodium, 0.03% modified Nano SiO
2, 0.03% monoglyceride, 1.2% shitosan, 0.06% metallothionein, 4% Rosmarinic acid, surplus to be mass percent concentration be 0.7% acetic acid aqueous solution.
This red shrimp antistaling agent obtains by the following method:
(1) modified Nano SiO is prepared
2: by Nano-meter SiO_2
2adding mass percent concentration is in the sodium dodecyl sulfate aqueous solution of 0.6%, Nano-meter SiO_2
2be 1:120 with the mass ratio of sodium dodecyl sulfate aqueous solution, regulate pH to be after 4.5, stir 7h, filter, wash with water, finally by dry, grind and obtain modified Nano SiO
2, stand-by;
(2) mixture: after taking each component respectively by above-mentioned mass percent, by modified Nano SiO
2add in acetic acid aqueous solution, after ultrasonic disperse is even, add shitosan, add monoglyceride after abundant dissolving, finally add glucose oxidase, glucose, dithiothreitol (DTT), nisin, casein sodium, metallothionein and Rosmarinic acid stir after degassed 12min under vacuum.
Embodiment 3
A kind of red shrimp antistaling agent, is made up of the component of following mass percent: 0.03% glucose oxidase, 3% glucose, 0.5% dithiothreitol (DTT), 0.05% nisin, 1% casein sodium, 0.05% modified Nano SiO
2, 0.05% monoglyceride, 2% shitosan, 0.1% metallothionein, 5% Rosmarinic acid, surplus to be mass percent concentration be 1% acetic acid aqueous solution.
This red shrimp antistaling agent obtains by the following method:
(1) modified Nano SiO is prepared
2: by Nano-meter SiO_2
2adding mass percent concentration is in the sodium dodecyl sulfate aqueous solution of 0.7%, Nano-meter SiO_2
2be 1:150 with the mass ratio of sodium dodecyl sulfate aqueous solution, regulate pH to be after 5, stir 8h, filter, wash with water, finally by dry, grind and obtain modified Nano SiO
2, stand-by;
(2) mixture: after taking each component respectively by above-mentioned mass percent, by modified Nano SiO
2add in acetic acid aqueous solution, after ultrasonic disperse is even, add shitosan, add monoglyceride after abundant dissolving, finally add glucose oxidase, glucose, dithiothreitol (DTT), nisin, casein sodium, metallothionein and Rosmarinic acid stir after degassed 15min under vacuum.
Red shrimp after fishing for is cleaned and drains, be placed in antistaling agent of the present invention and soak 5 ~ 10min, the mass ratio of red shrimp and antistaling agent is 1:5 ~ 10, pull out after draining film forming and carry out ice preservation, after preservation 10d, the basic Mei You of red shrimp ?become occur, shrimp body light pinkiness, good springiness, come off without carapace, smell is normal, VBN is at about 23mg/100g, illustrate that antistaling agent of the present invention has the antibacterial of excellence concurrently, fresh-keeping, protect look, anti-black change effect, fading of red shrimp can be suppressed simultaneously, ?become, putrid and deteriorated and carapace comes off, red shrimp fresh keeping time can be extended, productivity effect for the commodity value and operation on the sea that improve red shrimp is significant, be applicable to large-scale promotion application.
Above-described embodiment is one of the present invention preferably scheme, not does any pro forma restriction to the present invention, also has other variant and remodeling under the prerequisite not exceeding the technical scheme described in claim.
Claims (3)
1. a red shrimp antistaling agent, is characterized in that, is made up of the component of following mass percent: 0.01 ~ 0.03% glucose oxidase, 1 ~ 3% glucose, 0.3 ~ 0.5% dithiothreitol (DTT), 0.03 ~ 0.05% nisin, 0.5 ~ 1% casein sodium, 0.01 ~ 0.05% modified Nano SiO
2, 0.02 ~ 0.05% monoglyceride, 1 ~ 2% shitosan, 0.05 ~ 0.1% metallothionein, 3 ~ 5% Rosmarinic acids, surplus to be mass percent concentration be 0.5 ~ 1% acetic acid aqueous solution.
2. the red shrimp antistaling agent of the one according to claim, is characterized in that, described modification SiO
2obtain by the following method: by Nano-meter SiO_2
2adding mass percent concentration is in the sodium dodecyl sulfate aqueous solution of 0.5 ~ 0.7%, Nano-meter SiO_2
2be 1:100 ~ 150 with the mass ratio of sodium dodecyl sulfate aqueous solution, regulate pH to be after 4 ~ 5, stir 6 ~ 8h, filter, wash with water, finally by dry, grind and obtain modified Nano SiO
2.
3. a preparation method for red shrimp antistaling agent as claimed in claim 1, it is characterized in that, concrete steps are:
(1) modified Nano SiO is prepared
2: by Nano-meter SiO_2
2adding mass percent concentration is in the sodium dodecyl sulfate aqueous solution of 0.5 ~ 0.7%, Nano-meter SiO_2
2be 1:100 ~ 150 with the mass ratio of sodium dodecyl sulfate aqueous solution, regulate pH to be after 4 ~ 5, stir 6 ~ 8h, filter, wash with water, finally by dry, grind and obtain modified Nano SiO
2, stand-by;
(2) mixture: after taking each component respectively by above-mentioned mass percent, by modified Nano SiO
2add in acetic acid aqueous solution, after ultrasonic disperse is even, add shitosan, add monoglyceride after abundant dissolving, finally add glucose oxidase, glucose, dithiothreitol (DTT), nisin, casein sodium, metallothionein and Rosmarinic acid stir after degassed 10 ~ 15min under vacuum.
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| CN106305975A (en) * | 2016-08-23 | 2017-01-11 | 北海金不换水产贸易有限公司 | Preservative for clams |
| CN107307062A (en) * | 2017-08-08 | 2017-11-03 | 福建农林大学 | A kind of konjaku glucomannan shrimp antistaling agent and preparation method thereof |
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| CN104872269B (en) | 2018-05-22 |
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