CN109169639B - Tobacco rhizome disease dipping specimen preservation solution and specimen preparation method - Google Patents
Tobacco rhizome disease dipping specimen preservation solution and specimen preparation method Download PDFInfo
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- CN109169639B CN109169639B CN201810903663.2A CN201810903663A CN109169639B CN 109169639 B CN109169639 B CN 109169639B CN 201810903663 A CN201810903663 A CN 201810903663A CN 109169639 B CN109169639 B CN 109169639B
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- rhizome disease
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
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- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a tobacco rhizome disease dipping specimen preserving fluid and a specimen preparation method, wherein the tobacco rhizome disease dipping specimen preserving fluid comprises the following components: 0.05-0.07% of sodium hypochlorite, 6.5-7.5% of glacial acetic acid, 13-14.5% of alcohol, 0.3-0.5% of ascorbic acid and 0.05-0.15% of clove oil. The method comprises the following steps: (1) cleaning tobacco diseased roots and stems with tap water, and soaking the tobacco diseased roots and stems in 6% copper sulfate diluent for 28-32 hours; (2) taking out the rootstocks treated in the step (1) and preserving the rootstocks in the tobacco rootstock disease dipping specimen preserving fluid. The five components of sodium hypochlorite, glacial acetic acid, alcohol, ascorbic acid, clove oil and the like jointly act to preserve the original color and the shape of the tobacco rhizome disease specimen, and the reasonable concentration percentage of the five components plays a very important role in long-term stable preservation of the tobacco rhizome disease specimen without fading and peeling.
Description
Technical Field
The invention belongs to the technical field of specimen preparation, and particularly relates to tobacco rhizome disease dipping specimen preserving fluid and a preparation method thereof.
Background
The existing tobacco rootstock disease dipping specimens have more preservation methods, wherein the two methods are mainly adopted. One method is that tobacco rhizome disease specimen is put into copper acetate crystal and acetic acid mixed diluent for water boiling and then put into formalin and alcohol mixed solution for preservation; and the other method is that the tobacco rhizome disease sample is placed in copper sulfate solution, and the rhizome disease sample is boiled in water and then is placed in sulfurous acid and alcohol mixed solution for preservation. These two methods for preserving specimens by dipping have the following problems: (1) the heating treatment liquid can volatilize and emit pungent smell in the treatment process of the disease specimen, and the health of people is seriously affected. (2) The main components of the disease specimen preserving fluid, namely formalin and sulfurous acid, have certain toxicity. Formalin has a strong irritant effect on the eyes and the nose of a human, and in the list of carcinogens, sulfurous acid has a strong irritant effect on the eyes, the skin, the mucous membranes and the respiratory tract. (3) The original color of the tobacco rootstock disease specimen is not easy to preserve due to the stimulation effect of formalin and sulfurous acid on the tobacco rootstock disease specimen. (4) The phenomena of peeling, fading and the like can occur after the storage time is short, so that the disease spots are not clear, and the disease characteristics are not obvious.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: (1) at present, formalin and sulfurous acid in the dipping process of tobacco rhizome disease specimens have influence on human health, and dipping residual liquid has influence on the environment. (2) The root and stem disease specimen of the tobacco is peeled and faded after being stored for a long time, and the disease spots cannot be clearly distinguished after the root and stem disease specimen is stored for a short time. (3) Heating in the treatment process can give off pungent odor.
The technical scheme of the invention is as follows: a tobacco rhizome disease dipping specimen preservation solution comprises the following components in percentage by mass: 0.05-0.07% of sodium hypochlorite, 6.5-7.5% of glacial acetic acid, 13-14.5% of alcohol, 0.3-0.5% of ascorbic acid, 0.05-0.15% of clove oil and the balance of water.
On the other hand, the invention also provides a manufacturing method of the tobacco rhizome disease specimen, which comprises the following steps:
(1) cleaning tobacco diseased roots and stems with tap water, and soaking the tobacco diseased roots and stems in 6% copper sulfate diluent for 28-32 hours;
(2) taking out the rootstocks treated in the step (1) and preserving the rootstocks in the tobacco rootstock disease dipping specimen preserving fluid.
Compared with the prior art, the invention has the following beneficial effects:
the tobacco rhizome disease specimen is soaked in a copper sulfate diluent for sterilization, and then is put into a mixed solution consisting of sodium hypochlorite, glacial acetic acid, alcohol, ascorbic acid and clove oil for preservation. The invention is safe, simple in preparation and long in specimen preservation time, and can show the symptoms of different parts and different stages. The five components of sodium hypochlorite, glacial acetic acid, alcohol, ascorbic acid, clove oil and the like jointly act to preserve the original color and the shape of the tobacco rhizome disease specimen, and the reasonable concentration percentage of the five components plays a very important role in long-term stable preservation of the tobacco rhizome disease specimen without fading and peeling.
Detailed Description
Tobacco root and stem disease specimens were stored by dipping in the samples of examples 1 to 5 and comparative examples 1 to 2, respectively, in the same storage apparatus and under the same storage conditions. Each group was set to 5 replicates for a total of three replicates. The condition of the specimen is observed every day, whether the specimen fades or not and whether the scab is obvious or not are observed. Counting the time of the tobacco rhizome disease specimen fading and peeling and the number of months for preservation, and finally, comprehensively evaluating the effect of each preservation method according to the fading, peeling and preservation time.
Example 1:
a tobacco rhizome disease dipping specimen preserving fluid and a manufacturing method thereof comprise the following steps:
1. the tobacco rhizome disease specimen is cleaned by tap water, slightly trimmed (so that the tobacco rhizome disease specimen is easy to put into a bottle and is attractive), and then soaked in 6% copper sulfate diluent for 28-32 hours according to the size and the old tender degree of the rhizome.
2. Taking out the tobacco rhizome disease specimen, and putting the tobacco rhizome disease specimen into a preserving solution consisting of 0.05% of sodium hypochlorite, 7.5% of glacial acetic acid, 14.5% of alcohol, 0.3% of ascorbic acid and 0.05% of clove oil.
Example 2:
a tobacco rhizome disease dipping specimen preserving fluid and a manufacturing method thereof comprise the following steps:
1. the tobacco rhizome disease specimen is cleaned by tap water, slightly trimmed (so that the tobacco rhizome disease specimen is easy to put into a bottle and is attractive), and then soaked in 6% copper sulfate diluent for 28-32 hours according to the size and the old tender degree of the rhizome.
2. Taking out tobacco rhizome disease specimen, and storing in a preservation solution composed of sodium hypochlorite 0.06%, glacial acetic acid 7.0%, alcohol 14.0%, ascorbic acid 0.4%, and oleum Caryophylli 0.1%.
Example 3:
a tobacco rhizome disease dipping specimen preserving fluid and a manufacturing method thereof comprise the following steps:
1. the tobacco rhizome disease specimen is cleaned by tap water, slightly trimmed (so that the tobacco rhizome disease specimen is easy to put into a bottle and is attractive), and then soaked in 6% copper sulfate diluent to soak the tobacco rhizome disease specimen for 28-32 hours according to the size and the tenderness of the tobacco rhizome disease specimen.
2. Taking out tobacco rhizome disease specimen, and storing in a preservation solution composed of sodium hypochlorite 0.07%, glacial acetic acid 6.5%, alcohol 13.5%, ascorbic acid 0.5%, and oleum Caryophylli 0.15%.
Example 4:
a tobacco rhizome disease dipping specimen preserving fluid and a manufacturing method thereof comprise the following steps:
1. the tobacco rhizome disease specimen is cleaned by tap water, slightly trimmed (so that the tobacco rhizome disease specimen is easy to put into a bottle and is attractive), and then soaked in 6% copper sulfate diluent to soak the tobacco rhizome disease specimen for 28-32 hours according to the size and the tenderness of the tobacco rhizome disease specimen.
2. Taking out the tobacco rhizome disease specimen, and putting the tobacco rhizome disease specimen into a preservation solution consisting of 0.07 percent of sodium hypochlorite, 6.5 percent of glacial acetic acid, 13.5 percent of alcohol and 0.15 percent of clove oil.
Example 5:
a tobacco rhizome disease dipping specimen preserving fluid and a manufacturing method thereof comprise the following steps:
1. the tobacco rhizome disease specimen is cleaned by tap water, slightly trimmed (so that the tobacco rhizome disease specimen is easy to put into a bottle and is attractive), and then soaked in 6% copper sulfate diluent to soak the tobacco rhizome disease specimen for 28-32 hours according to the size and the tenderness of the tobacco rhizome disease specimen.
2. Taking out tobacco rhizome disease specimen, and storing in a preservation solution composed of sodium hypochlorite 0.09%, glacial acetic acid 5.5%, alcohol 14.0%, ascorbic acid 0.25%, and oleum Caryophylli 0.15%.
Comparative example 1:
a tobacco rhizome disease dipping specimen preserving fluid and a manufacturing method thereof comprise the following steps:
1. the tobacco rhizome disease specimen is cleaned by tap water, slightly trimmed (so that the tobacco rhizome disease specimen is easy to put into a bottle and is attractive), and then soaked in 6% copper sulfate diluent for 28-32 hours according to the size and the old tender degree of the rhizome.
2. Taking out a tobacco rhizome disease specimen, and storing the tobacco rhizome disease specimen in a mixed solution of 20 ml of concentrated sulfuric acid, 1000 ml of water and 16 g of solid sodium sulfite which are fully and uniformly mixed.
Comparative example 2:
a tobacco rhizome disease dipping specimen preserving fluid and a manufacturing method thereof comprise the following steps:
1. the tobacco rhizome disease specimen is cleaned by tap water, slightly trimmed (so that the tobacco rhizome disease specimen is easy to put into a bottle and is attractive), and then soaked in 6% copper sulfate diluent for 28-32 hours according to the size and the old tender degree of the rhizome.
2. Taking out the tobacco rhizome disease specimen, and storing the tobacco rhizome disease specimen in a mixed solution of 25 ml of formaldehyde, 150 ml of alcohol and 1000 ml of water which are fully and uniformly mixed.
The effects of the embodiment are as follows:
comparison of preservation effects of tobacco rhizome disease specimen preservation solutions
The tobacco root and stem disease specimens prepared in examples 1 to 3 and the tobacco root and stem disease specimens prepared in comparative examples were stored and preserved under the same environmental conditions. Wherein, the tobacco root and stem disease specimens in the comparative examples have the phenomena of skin peeling, fading, unobvious lesion and the like within about 10 months. The samples in the preservation solutions of the embodiments 1 to 3 of the invention are still fresh as before after 24 months, the disease spots are obvious, and the fading phenomenon does not occur, the tobacco root and stem disease specimens of the embodiment 4 begin to fade after 10 months of preservation, the disease spots are not obvious, and the tobacco root and stem disease specimens of the embodiment 5 begin to fade slightly after 15 months of preservation.
Compared with the defects and shortcomings of the existing plant rhizome specimen in dipping preservation, the invention has the following beneficial effects: the method for manufacturing the tobacco rhizome disease specimen is simple to operate, the manufactured tobacco rhizome disease specimen is long in storage time, is fresh after 36 months, is green and environment-friendly, and reduces pollution of formaldehyde and sulfurous acid to the environment and influence on bodies of operators.
The above-mentioned embodiments only express the specific embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present application. It should be noted that, for those skilled in the art, without departing from the technical idea of the present application, several changes and modifications can be made, which are all within the protection scope of the present application.
Claims (2)
1. The tobacco rhizome disease dipping specimen preservation solution is characterized by comprising the following components in percentage by mass: 0.05-0.07% of sodium hypochlorite, 6.5-7.5% of glacial acetic acid, 13-14.5% of alcohol, 0.3-0.5% of ascorbic acid, 0.05-0.15% of clove oil and the balance of water.
2. The manufacturing method of the tobacco rhizome disease specimen is characterized by comprising the following steps:
(1) cleaning tobacco diseased roots and stems with tap water, and soaking the tobacco diseased roots and stems in 6% copper sulfate diluent for 28-32 hours;
(2) taking out the rootstocks treated in the step (1) and preserving them in the tobacco rootstock disease dipping specimen preserving solution as claimed in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810903663.2A CN109169639B (en) | 2018-08-09 | 2018-08-09 | Tobacco rhizome disease dipping specimen preservation solution and specimen preparation method |
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| CN201810903663.2A CN109169639B (en) | 2018-08-09 | 2018-08-09 | Tobacco rhizome disease dipping specimen preservation solution and specimen preparation method |
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| CN109169639A CN109169639A (en) | 2019-01-11 |
| CN109169639B true CN109169639B (en) | 2021-01-19 |
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| CN201810903663.2A Expired - Fee Related CN109169639B (en) | 2018-08-09 | 2018-08-09 | Tobacco rhizome disease dipping specimen preservation solution and specimen preparation method |
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| CN113170781A (en) * | 2021-04-16 | 2021-07-27 | 河南有道展览科技有限公司 | Plant-impregnated environment-friendly preservation solution |
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| CN103283718B (en) * | 2013-07-04 | 2015-10-07 | 云南农业大学 | A kind of tobacco diseases Slide processing |
| CN103404510A (en) * | 2013-08-22 | 2013-11-27 | 湖北省烟草公司恩施州公司 | Method for making tobacco rhizome disease specimens |
| CN103548818B (en) * | 2013-11-25 | 2015-08-19 | 中国热带农业科学院香料饮料研究所 | A kind of preparation method of fleshiness orchid dipping specimen |
| CN103766328B (en) * | 2014-01-14 | 2015-12-30 | 云南省烟草农业科学研究院 | A kind of preparation method of plant leaf blade dipping specimen |
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Inventor after: Liu Xu Inventor after: Chen Song Inventor after: Chen Qingdong Inventor after: Liu Zhenxing Inventor after: Zhang Zongjin Inventor after: Yan Fangfang Inventor after: Zhang Ruiping Inventor after: Zhang Yingjie Inventor after: Hu Wei Inventor after: Chen Qianying Inventor after: Lei Qiang Inventor after: Yu Wei Inventor after: Liu Changhua Inventor after: Xiao Jun Inventor after: Wu Bin Inventor after: Qu Jiankang Inventor after: Yu Xiangwen Inventor after: Li Bin Inventor before: Liu Xu Inventor before: Liu Changhua Inventor before: Xiao Jun Inventor before: Wu Bin Inventor before: Chen Song Inventor before: Chen Qingdong Inventor before: Hu Wei Inventor before: Chen Qianying |
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Effective date of registration: 20190606 Address after: 610000 No. 20 Jingjusi Road, Chengdu City, Sichuan Province Applicant after: INSTITUTE OF PLANT PROTECTION, SICHUAN ACADEMY OF AGRICULTURAL SCIENCES Applicant after: CHINA NATIONAL TOBACCO CORPORATION SICHUAN Applicant after: PANZHIHUA CORPORATION OF SICHUAN PROVINCE TOBACCO MONOPOLY ADMINISTRATION Address before: No. 20 Jingjusi Road, Chengdu City, Sichuan Province Applicant before: INSTITUTE OF PLANT PROTECTION, SICHUAN ACADEMY OF AGRICULTURAL SCIENCES |
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