CN103027033A - Cordyceps specimen preservation solution - Google Patents
Cordyceps specimen preservation solution Download PDFInfo
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- CN103027033A CN103027033A CN 201110294497 CN201110294497A CN103027033A CN 103027033 A CN103027033 A CN 103027033A CN 201110294497 CN201110294497 CN 201110294497 CN 201110294497 A CN201110294497 A CN 201110294497A CN 103027033 A CN103027033 A CN 103027033A
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- CN
- China
- Prior art keywords
- chinese caterpillar
- caterpillar fungus
- cordyceps
- color
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 241000190633 Cordyceps Species 0.000 title abstract description 9
- 239000003761 preservation solution Substances 0.000 title abstract 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 18
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000203 mixture Substances 0.000 claims abstract description 7
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims abstract description 6
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 claims abstract description 5
- 241000233866 Fungi Species 0.000 claims description 57
- 240000001307 Myosotis scorpioides Species 0.000 claims description 55
- 239000007788 liquid Substances 0.000 claims description 12
- 235000011187 glycerol Nutrition 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 abstract description 9
- OPQARKPSCNTWTJ-UHFFFAOYSA-L copper(ii) acetate Chemical compound [Cu+2].CC([O-])=O.CC([O-])=O OPQARKPSCNTWTJ-UHFFFAOYSA-L 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 15
- 238000004321 preservation Methods 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 239000011521 glass Substances 0.000 description 8
- 239000012153 distilled water Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 241001264174 Cordyceps militaris Species 0.000 description 5
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 5
- 239000004677 Nylon Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 229920001778 nylon Polymers 0.000 description 4
- 239000012188 paraffin wax Substances 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 241000894007 species Species 0.000 description 3
- 241000235349 Ascomycota Species 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 241000794914 Metarhizium indigoticum Species 0.000 description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical class [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 241001480006 Clavicipitaceae Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000221775 Hypocreales Species 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000257211 Sordariomycetidae Species 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a preparation method of a cordyceps specimen preservation solution. The formula includes 1.0% of acetic acid, 0.5% of copper acetate (the composition is added selectively according to the color of cordyceps), 1.5% of formaldehyde, 0.2-0.5% of sulphurous acid (the composition is added selectively according to the color of cordyceps), 2.0-5.0% of glycerol, and 0.5% of ethanol. The solution can not only maintain shapes of cordyceps, but also keep the color, so that the cordyceps specimens are better preserved.
Description
Technical field
The present invention relates to a kind of preservation formula of liquid that is suitable for the variety classes Chinese caterpillar fungus.
Background technology
Cordyceps Cordyceps (Fr.) Link is under the jurisdiction of mycota (Fungi), Ascomycota Ascomycota, Ascomycetes Ascomycetes, excrement shell bacterium subclass Sordariomycetidae, Hypocreales Hypocreales, Clavicipitaceae Clavicipitaceae (Kirk et al.2001).According to the statistics of holt justice hero and clear water grand ceremony (1983), the Chinese caterpillar fungus of whole world report has more than 350 kinds.Subsequently, the kind name that international Preliminary List of Fungi database was listed in 2003 has reached more than 485 kinds, adds the novel species of in recent years delivering both at home and abroad, and the Chinese caterpillar fungus that the whole world has been reported is nearly more than 500 kinds.Remove synonym and illegal nomen rejiciendum and move to outside the kind of other genus, existing Chinese caterpillar fungus should have about 400 kinds.
After cordyceps mycelia is invaded the insect host body, mostly form the fine and close sclerotium of meat, white, faint yellow, brown or black, the sclerotium mycelia of individual other kind as Meitan Chinese caterpillar fungus (Cordyceps indigotica Kobayasi et Shimizu) can be green; After sclerotium generates, under suitable condition, can form stroma, the quality of stroma can be divided into meat, cellulosic, wooden and keratin, for different Chinese caterpillar funguses, the color of stroma is metastable, and that the common color of stroma has is faint yellow, orange, orange red, brown, white or the black of pale red, redness, grey and various tones.
The store method of Chinese caterpillar fungus sample mainly contains two kinds, and a kind of is traditional exsiccata store method, and after the Chinese caterpillar fungus drying, the sclerotium metamorphosis is less, and form and the change color of stroma are obvious, and easily damages by worms or go mouldy, and is unfavorable for that sample preserves or follow-up study; Another method is to adopt preservation liquid to preserve, mainly be to adopt formalin to advance solution to preserve, the Chinese caterpillar fungus form does not change substantially, but the color of aweto sclerotium and stroma is taken off easily, some unsettled colors particularly are such as the color such as faint yellow, orange, orange red, red and green.
Thereby, set up and a kind ofly can keep the Chinese caterpillar fungus form, can try one's best again so that aweto sclerotium and stroma keep its original color, for the follow-up study of Chinese caterpillar fungus, particularly the voucher specimen as some New Species of Cordycepss seems especially important.The present invention adopts improvement to preserve liquid the Chinese caterpillar fungus sample is preserved, and can so that Chinese caterpillar fungus keeps original form, its color is maintained.By consulting pertinent literature and patent, do not see the patent about the Chinese caterpillar fungus specimen preserving liquid, the patent of related application has the invention such as Xie Minhao " a kind of specimen anti-corrosion stationary liquid (Chinese invention patent number of patent application: CN200710024048.6) and " preservation liquid for animal body specimen " of the invention such as Shi Jiangong (Chinese invention patent application number: CN98108129.0); this patent is for the characteristics of Chinese caterpillar fungus and the needs of research; adopt the specimen preserving liquid of improved formulations; can keep under the constant prerequisite of Chinese caterpillar fungus form; it is substantially constant to preserve its original color, and this is conducive to the research of Chinese caterpillar fungus and the preservation of sample.
Summary of the invention
The invention provides a kind of improvement and preserve liquid, be adapted to the preservation of variety classes Chinese caterpillar fungus.
The operating procedure of Chinese caterpillar fungus immersed specimen is as follows:
The preliminary treatment of 1 Chinese caterpillar fungus sample
At first remove the foreign material such as earth, and with clear water Chinese caterpillar fungus is cleaned; Secondly remove and wrap in the outer bacterium cover of aweto sclerotium, and clean with distilled water; The 3rd, if the Chinese caterpillar fungus color is green, use copper/saturated copper sulphate solution to soak after 12 hours, distilled water is cleaned.
Fixing of 2 Chinese caterpillar fungus samples
Use transparent nylon wire that the Chinese caterpillar fungus sample is fixed on the transparency glass plate that is fit to size.
The preparation of 3 Chinese caterpillar fungus soaks
Prescription: 1.0% acetic acid, 0.5% Schweinfurt green (choosing adds this composition according to the Chinese caterpillar fungus color), 1.5% formaldehyde, 0.2%～0.5% sulfurous acid (choosing adds this composition according to the Chinese caterpillar fungus color), 2.0～5.0% glycerine, 0.5% ethanol.After solution preparation is good, pour in the clear glass jars.
4 put into wide-mouth bottle with the Chinese caterpillar fungus sample that fixes, and cover lid uses paraffin with bottle sealing, and the sample of making is placed long preservation in the darkroom.
Key point of the present invention is: the Chinese caterpillar fungus kind has kind more than 400, kind is numerous, that its sclerotium and stroma color mostly are is faint yellow, orange, orange red, brown, white or the black of pale red, redness, grey and various tones, a few species is green, the present invention is by using the preservation liquid of improvement, can not only keep the Chinese caterpillar fungus form not change, its color is not taken off, so that the Chinese caterpillar fungus sample is preserved preferably.
The present invention's practicality easy and simple to handle can be used for the long preservation of different types of Chinese caterpillar fungus.
Embodiment
Following examples are used for explanation the present invention, but are not limitations of the present invention.
The making of example one Cordyceps militaris sample
Cordyceps militaris (Cordyceps militaris (L.exFr.) Link) stroma color is orange to orange red, and sclerotium appearance color is dark brown.
The preliminary treatment of 1 Chinese caterpillar fungus sample
At first remove the foreign material such as Cordyceps militaris appearance earth, and with clear water Chinese caterpillar fungus is cleaned; Secondly remove and wrap in the outer bacterium cover of aweto sclerotium, and clean with distilled water;
Fixing of 2 Chinese caterpillar fungus samples
Use transparent nylon wire that the Cordyceps militaris sample is fixed on the transparency glass plate that is fit to size.
The preparation of 3 Chinese caterpillar fungus soaks
Prescription: 1.0% acetic acid, 1.5% formaldehyde, 0.5% sulfurous acid, 5.0% glycerine, 0.5% ethanol.After solution preparation is good, pour in the clear glass jars.
4 put into wide-mouth bottle with the Chinese caterpillar fungus sample that fixes, and cover lid uses paraffin with bottle sealing, and the sample of making is placed long preservation in the darkroom.
The making of example two Cordyceps sinensis samples
Cordyceps sinensis (Cordyceps sinensis (Berk.) Sacc.) stroma partly is white to the lime look near sclerotium, and sclerotium appearance color is light yellow to buff.
The preliminary treatment of 1 Chinese caterpillar fungus sample
At first remove the foreign material such as Cordyceps sinensis appearance earth, and with clear water Chinese caterpillar fungus is cleaned; Secondly remove and wrap in the outer bacterium cover of aweto sclerotium, and clean with distilled water;
Fixing of 2 Chinese caterpillar fungus samples
Use transparent nylon wire that the Cordyceps sinensis sample is fixed on the transparency glass plate that is fit to size.
The preparation of 3 Chinese caterpillar fungus soaks
Prescription: 1% acetic acid, 1.5% formaldehyde, 0.2% sulfurous acid, 5% glycerine, 0.5% ethanol.After solution preparation is good, pour in the clear glass jars.
4 put into wide-mouth bottle with the Chinese caterpillar fungus sample that fixes, and cover lid uses paraffin with bottle sealing, and the sample of making is placed long preservation in the darkroom.
The making of example three Meitan Chinese caterpillar fungus samples
Meitan Chinese caterpillar fungus (Cordyceps indigotica Kobayasi et Shimizu) sclerotium and stroma are light green color.
The preliminary treatment of 1 Chinese caterpillar fungus sample
At first remove the foreign material such as Meitan Chinese caterpillar fungus appearance earth, and with clear water Chinese caterpillar fungus is cleaned; Secondly remove and wrap in the outer bacterium cover of aweto sclerotium, and clean with distilled water; Use afterwards copper/saturated copper sulphate solution to soak after 12 hours, distilled water is cleaned.
Fixing of 2 Chinese caterpillar fungus samples
Use transparent nylon wire that Meitan Chinese caterpillar fungus sample is fixed on the transparency glass plate that is fit to size.
The preparation of 3 Chinese caterpillar fungus soaks
Prescription: 1% acetic acid, 1.5% formaldehyde, 0.5% Schweinfurt green, 3% glycerine, 0.5% ethanol.After solution preparation is good, pour in the clear glass jars.
4 put into wide-mouth bottle with the Chinese caterpillar fungus sample that fixes, and cover lid uses paraffin with bottle sealing, and the sample of making is placed long preservation in the darkroom.
Claims (1)
1. Chinese caterpillar fungus specimen preserving liquid is characterized in that the method comprises:
(1) specimen preserving liquid prescription: 1.0% acetic acid, 0.5% Schweinfurt green (choosing adds this composition according to the Chinese caterpillar fungus color), 1.5% formaldehyde, 0.2%～0.5% sulfurous acid (choosing adds this composition according to the Chinese caterpillar fungus color), 2.0～5.0% glycerine, 0.5% ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110294497 CN103027033A (en) | 2011-10-08 | 2011-10-08 | Cordyceps specimen preservation solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110294497 CN103027033A (en) | 2011-10-08 | 2011-10-08 | Cordyceps specimen preservation solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103027033A true CN103027033A (en) | 2013-04-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110294497 Pending CN103027033A (en) | 2011-10-08 | 2011-10-08 | Cordyceps specimen preservation solution |
Country Status (1)
| Country | Link |
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| CN (1) | CN103027033A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766328A (en) * | 2014-01-14 | 2014-05-07 | 云南省烟草农业科学研究院 | Manufacturing method for plant leaf dipping specimen |
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2011
- 2011-10-08 CN CN 201110294497 patent/CN103027033A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766328A (en) * | 2014-01-14 | 2014-05-07 | 云南省烟草农业科学研究院 | Manufacturing method for plant leaf dipping specimen |
| CN103766328B (en) * | 2014-01-14 | 2015-12-30 | 云南省烟草农业科学研究院 | A kind of preparation method of plant leaf blade dipping specimen |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130410 |