CN108770840A - A kind of tobacco diseases leaf blade specimen dipping preserves liquid and Slide processing - Google Patents
A kind of tobacco diseases leaf blade specimen dipping preserves liquid and Slide processing Download PDFInfo
- Publication number
- CN108770840A CN108770840A CN201810907354.2A CN201810907354A CN108770840A CN 108770840 A CN108770840 A CN 108770840A CN 201810907354 A CN201810907354 A CN 201810907354A CN 108770840 A CN108770840 A CN 108770840A
- Authority
- CN
- China
- Prior art keywords
- tobacco
- liquid
- tobacco diseases
- acid
- leaf blade
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
-
- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/38—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for botany
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Business, Economics & Management (AREA)
- Educational Technology (AREA)
- Mathematical Analysis (AREA)
- Mathematical Optimization (AREA)
- Mathematical Physics (AREA)
- Pure & Applied Mathematics (AREA)
- Algebra (AREA)
- Botany (AREA)
- Educational Administration (AREA)
- Computational Mathematics (AREA)
- Theoretical Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
It is impregnated the invention discloses a kind of tobacco diseases leaf blade specimen and preserves liquid and Slide processing, it is by following mass percent at being grouped as：Peracetic acid 0.3-0.5%, ascorbic acid 0.2-0.4%, citric acid 0.5-1.5%.The production method of tobacco diseases leaf blade specimen, includes the following steps：(1) it will be put into 5% copper-bath and impregnate 12~24 hours after tobacco diseases blade originally water washing；(2) step (1) treated blade is taken out and is stored in the tobacco diseases leaf blade specimen of the present invention and preserves in liquid.Three kinds of Peracetic acid, ascorbic acid, citric acid ingredient collective effects stablize the internal organizational structure and physical property of tobacco leaf in the present invention.Percentage between them also functions to very crucial effect for the preservation steady in a long-term of tobacco leaf disease sample.
Description
Technical field
The invention belongs to plant specimen manufacture technology fields, are related to tobacco diseases leaf blade specimen dipping and preserve liquid and sample
Production method.
Background technology
Nicotiana Dicotyledoneae eggplant mesh Solanaceae Nicotiana, tobacco diseases sample teaching and scientific research are field diseases identification
Important tool.There are many store method of existing tobacco diseases leaf blade specimen, and there are commonly infusion processes and seasoning.But Tobacco Leaf
Piece is roomy, moisture content is more, is easy discoloration with the seasonings such as drying or drying and have, is protected poor color effect, scab unobvious and guarantor
Deposit the time it is short the shortcomings of.And infusion process is general：(1) use copper acetate crystallization and acetic acid mixed diluting liquid will be after disease blade boiling
It is placed in formalin and preserves；(2) use the dilution of cupric sulphate crystal that will be put into sulfurous acid, alcohol etc. after disease blade boiling
It is preserved in mixed liquor.Both methods problems with can all occur to impregnate preservation sample：(a) concentration for the treatment of fluid and when processing
Between need a period of time to grope just to can determine that；(b) process after blade comparatively fresh when occur blade atrophy, leaf color hair
Changing causes some processed rear scabs not see Chu, Disease Characters unobvious.(c) add during disease sample disposal
It can volatilize when heat treatment solution, distribute penetrating odor, seriously affect the health of people.(d) the disease leaf blade specimen time is preserved
It does not grow, easily fade.
Invention content
The technical problems to be solved by the invention are：Solve the existing tobacco diseases blade dipping specimen holding time more long afterwards
Disease leaf blade specimen fades, and the holding time is not grown, and a few weeks or months will change yellow, blackening, can not distinguish and locate
During reason the problems such as irritant smell.
The technical scheme is that：A kind of tobacco diseases leaf blade specimen dipping preservation liquid, it is by following mass percent
At being grouped as：Peracetic acid 0.3-0.5%, ascorbic acid 0.2-0.4%, citric acid 0.5-1.5%, surplus are water.
On the other hand, the present invention also provides a kind of production method of tobacco diseases leaf blade specimen, include the following steps：
(1) it will be put into 5% copper-bath and impregnate 12~24 hours after tobacco diseases blade originally water washing；
(2) step (1) treated blade is taken out and is stored in the tobacco diseases leaf blade specimen dipping of the present invention and preserves liquid
In.
Compared with prior art, the invention has the advantages that：
Be put into 5% copper sulphate dilution after soaking sterilization using by tobacco diseases leaf blade specimen, place into Peracetic acid,
It is preserved in the mixed liquor that ascorbic acid, citric acid form.Three kinds of Peracetic acid, ascorbic acid, citric acid ingredients are total in the present invention
Same-action stablizes the internal organizational structure and physical property of tobacco leaf.Percentage between them is for tobacco leaf disease
The preservation steady in a long-term of evil sample also functions to very crucial effect.
Specific implementation mode
Under same preservation utensil and same preservation condition, is impregnated respectively with Examples 1 to 5 and comparative example 1~2 and preserve cigarette
Blade of grass piece disease sample.Every group sets 5 repetitions, altogether in triplicate.Sample situation is recorded in observation daily, and whether there is or not fade, preserve liquid
Whether muddy, whether scab feature is apparent.The time that tobacco leaf disease sample fades, chlorosis occurs is counted, it is muddy to preserve liquid
And the months preserved, it fades in finally being preserved with tobacco diseases leaf blade specimen, preserve liquid muddiness, the length of holding time to integrate
Evaluate the effect of each preservation liquid and production method.
Embodiment 1：
A kind of tobacco leaf disease specimen preserving liquid and production method, include the following steps：
1. by the Peracetic acid of two-component packaging, will be put into after the mixing of two liquid of A, B by products instruction requirement using preceding
Under the conditions of 30 DEG C, it is closed stand 48 hours after obtain stoste, stoste is configured to pure water to 0.3% dilution, is added
Ascorbic acid, citric acid are configured to dipping and preserve liquid.Dipping preserves liquid by Peracetic acid 0.3%, ascorbic acid 0.4%, lemon
Acid 0.5% forms.
It is placed in 5% copper-bath 2. tobacco leaf disease sample tap water is cleaned, optic lobe piece tender degree always
It is taken out after impregnating 12~24 hours.
3. being stored in dipping after tobacco diseases sample is taken out to preserve in liquid.
Embodiment 2：
A kind of tobacco leaf disease specimen preserving liquid and production method, include the following steps：
1. by the Peracetic acid of two-component packaging, will be put into after the mixing of two liquid of A, B by products instruction requirement using preceding
Under the conditions of 30 DEG C, it is closed stand 48 hours after obtain stoste, stoste is configured to pure water to 0.4% dilution, is added
Ascorbic acid, citric acid are configured to dipping and preserve liquid.Dipping preserves liquid by Peracetic acid 0.4%, ascorbic acid 0.3%, lemon
Acid 1.0% forms.
It is placed in 5% copper-bath 2. tobacco leaf disease sample tap water is cleaned, optic lobe piece tender degree always
It is taken out after impregnating 12~24 hours.
3. being stored in dipping after tobacco diseases sample is taken out to preserve in liquid.
Embodiment 3：
A kind of tobacco leaf disease specimen preserving liquid and production method, include the following steps：
1. by the Peracetic acid of two-component packaging, will be put into after the mixing of two liquid of A, B by products instruction requirement using preceding
Under the conditions of 30 DEG C, it is closed stand 48 hours after obtain stoste, stoste is configured to pure water to 0.5% dilution, is added
Ascorbic acid, citric acid are configured to dipping and preserve liquid.Dipping preserves liquid by Peracetic acid 0.5%, ascorbic acid 0.2%, lemon
Acid 1.5% forms.
It is placed in 5% copper-bath 2. tobacco leaf disease sample tap water is cleaned, optic lobe piece tender degree always
It is taken out after impregnating 12~24 hours.
3. being stored in dipping after tobacco diseases sample is taken out to preserve in liquid.
Embodiment 4：
A kind of tobacco leaf disease specimen preserving liquid and production method, include the following steps：
1. by the Peracetic acid of two-component packaging, will be put into after the mixing of two liquid of A, B by products instruction requirement using preceding
Under the conditions of 30 DEG C, it is closed stand 48 hours after obtain stoste, stoste is configured to pure water to 0.5% dilution, is added
Ascorbic acid, citric acid are configured to dipping and preserve liquid.Dipping preserves liquid by Peracetic acid 0.5%, alcohol 15%, citric acid
0.5% composition.
It is placed in 5% copper-bath 2. tobacco leaf disease sample tap water is cleaned, optic lobe piece tender degree always
It is taken out after impregnating 12~24 hours.
3. being stored in dipping after tobacco diseases sample is taken out to preserve in liquid.
Embodiment 5：
A kind of tobacco leaf disease specimen preserving liquid and production method, include the following steps：
1. by the Peracetic acid of two-component packaging, will be put into after the mixing of two liquid of A, B by products instruction requirement using preceding
Under the conditions of 30 DEG C, it is closed stand 48 hours after obtain stoste, stoste is configured to pure water to 0.5% dilution, is added
Ascorbic acid, citric acid are configured to dipping and preserve liquid.Dipping preserves liquid by Peracetic acid 0.4%, ascorbic acid 0.1%, lemon
Acid 0.5% forms.
It is placed in 5% copper-bath 2. tobacco leaf disease sample tap water is cleaned, optic lobe piece tender degree always
It is taken out after impregnating 12~24 hours.
3. being stored in dipping after tobacco diseases sample is taken out to preserve in liquid.
Comparative example 1：
A kind of plant leaf blade disease specimen preserving liquid and production method, include the following steps：
It is placed in 5% copper-bath 1. tobacco leaf disease sample tap water is cleaned, optic lobe piece tender degree always
It is taken out after impregnating 12~24 hours.
2. being stored in the mixed liquor of formaldehyde 2.5%, alcohol 15%.
Comparative example 2：
A kind of plant leaf blade disease specimen preserving liquid and production method, include the following steps：
It is placed in 5% copper-bath 1. tobacco leaf disease sample tap water is cleaned, optic lobe piece tender degree always
It is taken out after impregnating 12~24 hours.
2. being stored in sulfurous acid 1.5%.
Embodiment effect：
Tobacco leaf disease specimen preserving liquid preservation effect compares
| Store method | Sample situation | Preserve liquid color | Scab feature | Holding time (moon) |
| Embodiment 1 | Have no colour fading chlorosis | Have no muddy | Obviously | 24 |
| Embodiment 2 | Have no colour fading chlorosis | Have no muddy | Obviously | 24 |
| Embodiment 3 | Have no colour fading chlorosis | Have no muddy | Obviously | 24 |
| Embodiment 4 | Start within 6 months colour fading chlorosis | Start within 7 months muddy | It is unintelligible | 7 |
| Embodiment 5 | Start within 13 months colour fading chlorosis | Start within 14 months muddy | It is unintelligible | 14 |
| Comparative example 1 | Start within 12 months colour fading chlorosis | Start within 13 months muddy | It is less clear | 13 |
| Comparative example 2 | Start within 11 months colour fading chlorosis | Start within 12 months muddy | It is less clear | 12 |
Exist to tobacco sample made in tobacco leaf disease sample and comparative example made in Examples 1 to 3
It stores and preserves under identical environmental condition.Leaf blade specimen wherein in comparative example preserves 11~12 months blades with regard to gradual in storage
It fades, scab is gradually thin out, unintelligible.And the tobacco leaf disease sample color that the embodiment of the present invention 1~3 preserves in liquid does not have
Change, scab is still apparent.Embodiment 4 begins to colour fading, chlorosis for 6 months, preserves liquid just within 7 months due to not adding ascorbic acid
It becomes cloudy, the holding time is 7 months.Embodiment 5 starts colour fading, chlorosis after 13 months, and scab is less clear.
The shortcomings that existing plant leaf blade specimen preserves liquid and deficiency are compared, the invention has the advantages that：The present invention
Tobacco leaf disease Slide processing it is easy to operate, the made tobacco leaf disease Saving specimen time is longer.It is protecting
Be not in the problem of leaf blade specimen fades during depositing, and environmentally protective, reduce pollution to environment and to operator
The influence of member's body.
Claims (2)
1. a kind of tobacco diseases leaf blade specimen dipping preserves liquid, which is characterized in that it is by following mass percent at being grouped as：
Peracetic acid 0.3-0.5%, ascorbic acid 0.2-0.4%, citric acid 0.5-1.5%, surplus are water.
2. a kind of production method of tobacco diseases leaf blade specimen, which is characterized in that include the following steps：
(1) it will be put into 5% copper-bath and impregnate 12~24 hours after tobacco diseases blade originally water washing；
(2) step (1) treated blade taking-up tobacco diseases leaf blade specimen dipping described in claim 1 is stored in preserve
In liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810907354.2A CN108770840B (en) | 2018-08-09 | 2018-08-09 | Tobacco disease leaf specimen dipping preservation liquid and specimen preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810907354.2A CN108770840B (en) | 2018-08-09 | 2018-08-09 | Tobacco disease leaf specimen dipping preservation liquid and specimen preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108770840A true CN108770840A (en) | 2018-11-09 |
| CN108770840B CN108770840B (en) | 2021-01-19 |
Family
ID=64028600
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810907354.2A Expired - Fee Related CN108770840B (en) | 2018-08-09 | 2018-08-09 | Tobacco disease leaf specimen dipping preservation liquid and specimen preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108770840B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102379280B (en) * | 2011-08-05 | 2013-07-31 | 云南省烟草农业科学研究院 | Tobacco leaf specimen treatment liquid and flue-cured tobacco leaf specimen batch production and preservation method |
| CN103283718A (en) * | 2013-07-04 | 2013-09-11 | 云南农业大学 | Method for manufacturing tobacco disease specimen |
| CN103404510A (en) * | 2013-08-22 | 2013-11-27 | 湖北省烟草公司恩施州公司 | Method for making tobacco rhizome disease specimens |
| CN103548818A (en) * | 2013-11-25 | 2014-02-05 | 中国热带农业科学院香料饮料研究所 | Preparation method for orchidaceae succulent plant dipping specimen |
| CN103766328A (en) * | 2014-01-14 | 2014-05-07 | 云南省烟草农业科学研究院 | Manufacturing method for plant leaf dipping specimen |
-
2018
- 2018-08-09 CN CN201810907354.2A patent/CN108770840B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102379280B (en) * | 2011-08-05 | 2013-07-31 | 云南省烟草农业科学研究院 | Tobacco leaf specimen treatment liquid and flue-cured tobacco leaf specimen batch production and preservation method |
| CN103283718A (en) * | 2013-07-04 | 2013-09-11 | 云南农业大学 | Method for manufacturing tobacco disease specimen |
| CN103404510A (en) * | 2013-08-22 | 2013-11-27 | 湖北省烟草公司恩施州公司 | Method for making tobacco rhizome disease specimens |
| CN103548818A (en) * | 2013-11-25 | 2014-02-05 | 中国热带农业科学院香料饮料研究所 | Preparation method for orchidaceae succulent plant dipping specimen |
| CN103766328A (en) * | 2014-01-14 | 2014-05-07 | 云南省烟草农业科学研究院 | Manufacturing method for plant leaf dipping specimen |
Non-Patent Citations (3)
| Title |
|---|
| 张永富 等: "烟草病害标本制作方法", 《中国烟草》 * |
| 朱辉 等: "棕榈植物病害浸渍标本保绿技术研究", 《中国热带农业》 * |
| 黄艳花 等: "植物病害标本固绿保绿技术研究", 《安徽农业科学》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108770840B (en) | 2021-01-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101830014B (en) | Method for preventing bamboo wood from mildewing | |
| Seyf et al. | Study on the effect of aluminum sulfate treatment on postharvest life of the cut rose'Boeing'(Rosa hybrida cv. Boeing). | |
| CN103548818B (en) | A kind of preparation method of fleshiness orchid dipping specimen | |
| CN105875210A (en) | Method for grafting bergamots | |
| Abou El-Ghait et al. | Effect of some postharvest treatments on vase life and quality of chrysanthemum (Dendranthema grandiflorum Kitam) cut flowers | |
| CN106070348A (en) | A kind of Fructus Citri Sarcodactylis graft union agent and preparation method thereof | |
| Langroudi et al. | Effects of silver nanoparticles, chemical treatments and herbal essential oils on the vase life of cut alstroemeria (Alstroemeria ‘Summer Sky’) flowers | |
| Amani Beni et al. | Improving physiological quality of cut tuberose (Polianthes tuberosa cv. Single) flowers by continues treatment with humic acid and nano-silver particles | |
| Kazaz et al. | Vase life extension of cut hydrangea (Hydrangea macrophylla) flowers | |
| CN108770840A (en) | A kind of tobacco diseases leaf blade specimen dipping preserves liquid and Slide processing | |
| EP0018341B1 (en) | A method and composition for the preservation of plants | |
| AU2019101540A4 (en) | Use of Schiff Base Derivative from Dehydroabietylamine and (Substituted) Benzaldehyde | |
| CN109169639B (en) | Tobacco rhizome disease dipping specimen preservation solution and specimen preparation method | |
| Minde | The effects of different vase solutions on the postharvest life of rose flower-review | |
| Othman et al. | Enhancing vase life of Helianthus annuus L. cut flowers using salicylic acid and dill essential oil | |
| WO2018062543A1 (en) | Method for producing indigo leaf solution containing tryptanthrin | |
| CN107820898A (en) | A kind of method for extending the lily flower arrangement time | |
| CN103704038A (en) | Preservation method for harvested persimmons | |
| El-Kafie et al. | PHYSIOLOGICAL STUDIES ON POST HARVEST OF Chrysanthemum morifolium, L. CV | |
| CN1262181C (en) | Common freesia cutting flower pretreating liquid and its using method | |
| CN108718764A (en) | A kind of potting preservation technology and its application method | |
| Taha | The Longevity and Quality of Tuberose (Polianthes tuberosa L.) Cut Flowers as Affected by Ethanol, Methanol, Citric Acid and Silver Thiosulphate | |
| KR102610350B1 (en) | Composition Containing Mixture of Sucrose, Indole-3-acetic Acid and Rose-Hip Extract For Extending Vase Life and Water Loss of Cut Flowers | |
| Press | Effect of duckweed meal on the rate of mold infestation in stored pelleted fish feed | |
| Abou-Elwafa et al. | Effect of postharvest exogenous edible coating treatments on inhibitor browning and maintaining quality of fresh mushroom |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Xu Inventor after: Chen Song Inventor after: Chen Qingdong Inventor after: Liu Zhenxing Inventor after: Zhang Zongjin Inventor after: Yan Fangfang Inventor after: Zhang Ruiping Inventor after: Zhang Yingjie Inventor after: Hu Wei Inventor after: Chen Qianying Inventor after: Lei Qiang Inventor after: Yu Wei Inventor after: Liu Changhua Inventor after: Xiao Jun Inventor after: Wu Bin Inventor after: Qu Jiankang Inventor after: Yu Xiangwen Inventor after: Li Bin Inventor before: Liu Xu Inventor before: Liu Changhua Inventor before: Xiao Jun Inventor before: Wu Bin Inventor before: Chen Song Inventor before: Chen Qingdong Inventor before: Hu Wei Inventor before: Chen Qianying |
|
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20190520 Address after: 610000 No. 20 Jingjusi Road, Chengdu City, Sichuan Province Applicant after: INSTITUTE OF PLANT PROTECTION, SICHUAN ACADEMY OF AGRICULTURAL SCIENCES Applicant after: CHINA NATIONAL TOBACCO CORPORATION SICHUAN Applicant after: PANZHIHUA CORPORATION OF SICHUAN PROVINCE TOBACCO MONOPOLY ADMINISTRATION Address before: No. 20 Jingjusi Road, Chengdu City, Sichuan Province Applicant before: INSTITUTE OF PLANT PROTECTION, SICHUAN ACADEMY OF AGRICULTURAL SCIENCES |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210119 Termination date: 20210809 |