CN108432740A - A kind of preservation liquid of sample - Google Patents
A kind of preservation liquid of sample Download PDFInfo
- Publication number
- CN108432740A CN108432740A CN201810307048.5A CN201810307048A CN108432740A CN 108432740 A CN108432740 A CN 108432740A CN 201810307048 A CN201810307048 A CN 201810307048A CN 108432740 A CN108432740 A CN 108432740A
- Authority
- CN
- China
- Prior art keywords
- parts
- sample
- preservation liquid
- glutaraldehyde
- sodium chloride
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
Abstract
The invention discloses a kind of preservation liquid of sample, are mixed by weight ratio by following raw material：35 parts of potassium sorbate, 57 parts of industrial alcohol, 13 parts of Laurocapram, 13 parts of ethylenediamine tetraacetic acid disodium zinc salt salt, 0.8 1.2 parts of protein-crosslinking agent, 12 parts of bleeding agent, 13 parts of glycerine, 12 parts of glutaraldehyde, 79 parts of sodium chloride, 8 12 parts of citric acid, 80 100 parts of deionized water.Specimen preserving liquid of the present invention is the liquid of water white transparency, is had no irritating odor, and fungus and mildew resistance is had excellent performance；The tissue morphology and color and luster of protozoa sample can be effectively kept, paint having no volatile substances, effect is lasting, can be effectively reduced cost；And poisonous and harmful substance is free of, and it is environmentally protective, mitigate the burden of environment.
Description
Technical field
The present invention relates to pathological tissue Techniques of preserving field, the preservation liquid of specifically a kind of sample.
Background technology
From operating room to pathology department, clinical laboratory's surgery, tissue biopsy, histopathology section office, gastroscope section office etc. need to group
It knits or the fixation of cell etc., stores, keeps sample, be required for using specimen preserving liquid, existing preservations liquid being mainly formalin guarantor
Liquid storage.Formalin specimen preserving liquid has good fixed function, this is because the functional group of formaldehyde and sample protein is big
Amount combines, and long-term placement further polymerization forms paraformaldehyde.This process is allowed to form appropriateness in protein peptide interchain
Crosslinking, can sterilize and keep the tissue morphology of sample.But formaldehyde, benzene class are determined as causing by the World Health Organization
Cancer substance is listed in high poison chemicals, meeting intense stimulus eyes, skin, respiratory mucosa etc., it is different to ultimately cause immune function
Often, hepatic injury and central nervous system are affected, will cause fetal anomaly, leukaemia, chronic respiratory disease, nasopharyngeal carcinoma,
The diseases such as feminine menstrual disorder, acute confusion depression, serious threat is caused to health.Simultaneously because formalin
Low boiling point, therefore the highly volatile in room temperature are accelerated with the rising evaporation rate of temperature, give out strong impulse smell,
Serious pollution laboratory environment.Over time, air formaldehyde concentration also constantly increases, and to medicine, biology and moves
The health of object worker, which causes, to be seriously threatened.In addition, the sample through formalin long-term preservation, it may occur that go mouldy, xanthochromia,
Phenomena such as sample is hardened.
Invention content：
The object of the present invention is to provide a kind of preservation liquid of sample.The preservation liquid is free of formaldehyde and formalin, volatilization
Slowly, it has no irritating odor, toxicity is very low, safe, will not pollute experimental situation, and preservation effect is good, and the long period preserves not
It can go mouldy, sample soft texture, and color and luster is good.
In order to solve the above technical problems, the present invention is realized using following technical scheme：
A kind of preservation liquid of sample, is mixed by weight ratio by following raw material：
3-5 parts of potassium sorbate, 5-7 parts of industrial alcohol, 1-3 parts of Laurocapram, 1-3 parts of ethylenediamine tetraacetic acid disodium zinc salt salt, egg
White 0.8-1.2 parts of crosslinking agent, 1-2 parts of bleeding agent, 1-3 parts of glycerine, 1-2 parts of glutaraldehyde, 7-9 parts of sodium chloride, 8-12 parts of citric acid,
80-100 parts of deionized water.
Further, the preservation liquid of a kind of sample, is mixed by weight ratio by following raw material：
3 parts of potassium sorbate, 5 parts of industrial alcohol, 1 part of Laurocapram, 1 part of ethylenediamine tetraacetic acid disodium zinc salt salt, protein-crosslinking agent
0.8 part, 1 part of bleeding agent, 1 part of glycerine, 1 part of glutaraldehyde, 7 parts of sodium chloride, 8 parts of citric acid, 80 parts of deionized water.
Further, the preservation liquid of a kind of sample, is mixed by weight ratio by following raw material：
4 parts of potassium sorbate, 6 parts of industrial alcohol, 2 parts of Laurocapram, 2 parts of ethylenediamine tetraacetic acid disodium zinc salt salt, protein-crosslinking agent 1
Part, 1.5 parts of bleeding agent, 2 parts of glycerine, 1.5 parts of glutaraldehyde, 8 parts of sodium chloride, 10 parts of citric acid, 90 parts of deionized water.
Further, the preservation liquid of a kind of sample, is mixed by weight ratio by following raw material：
5 parts of potassium sorbate, 7 parts of industrial alcohol, 3 parts of Laurocapram, 3 parts of ethylenediamine tetraacetic acid disodium zinc salt salt, protein-crosslinking agent
1.2 parts, 2 parts of bleeding agent, 3 parts of glycerine, 2 parts of glutaraldehyde, 9 parts of sodium chloride, 12 parts of citric acid, 100 parts of deionized water.
The potassium sorbate is colourless flake shape crystalline powder, odorless, tasteless, soluble easily in water, ethyl alcohol.Potassium sorbate has
There are efficient anti-corrosive properties and bacteriostasis, and better heat stability, decomposition temperature to be up to 270 DEG C, the low advantage of toxicity, is state
Low toxicity, the efficient fresh-keeping preservative of border food and agricultural organization and the World Health Organization to various countries' keypoint recommendation；It to enzyme bacterium, saccharomycete,
Aerobic microorganism has obvious inhibiting effect, and for antibacterial optimum pH in 5.0-6.0, fungistatic effect is 5-10 times higher than benzoic acid,
Toxicity is only 1/5th of benzoic acid；And have no adverse effects to flavour of food products, be widely used in meat, fish, egg, bird product,
The anti-corrosive fresh-keeping of grape wine, fruit wine, dairy products etc..Anti-corrosion is played to the preservation of human body specimen, is sterilized, primitive organism sample is kept
The color and luster and wettability of tissue, prevent the effect gone mouldy.
The protein-crosslinking agent is one or more of malonaldehyde, glyoxal, butanedial composition.
The bleeding agent is one or more of sodium chloride, sodium nitrate, potassium chloride composition.
The mass fraction of the glutaraldehyde is 48-52%.
The citric acid is non-toxic and tasteless organic acid can effectively kill blood with very strong sterilization and antioxidation
The bacterial spore polluted in liquid dialysis machine pipeline has efficient antibacterial and holding biological sample primary colors to the preservation of biological sample
The effect in pool is conducive to the preparation to preserving fluid for biological sample.
Compared with the prior art, the advantages of the present invention are as follows：Specimen preserving liquid of the present invention is the liquid of water white transparency, stingless
Swash property smell, fungus and mildew resistance is had excellent performance；The tissue morphology and color and luster of protozoa sample, non-volatility object can be effectively kept
Matter, effect is lasting, can be effectively reduced cost；And poisonous and harmful substance is free of, and it is environmentally protective, mitigate the burden of environment.
Specific implementation mode
The content for using specific embodiment to further illustrate the present invention below.
Embodiment 1
A kind of preservation liquid of sample, is mixed by weight ratio by following raw material：
3 parts of potassium sorbate, 5 parts of industrial alcohol, 1 part of Laurocapram, 1 part of ethylenediamine tetraacetic acid disodium zinc salt salt, protein-crosslinking agent
0.8 part, 1 part of bleeding agent, 1 part of glycerine, 1 part of glutaraldehyde, 7 parts of sodium chloride, 8 parts of citric acid, 80 parts of deionized water.
The protein-crosslinking agent be malonaldehyde, glyoxal composition.
The bleeding agent is the composition of sodium chloride, sodium nitrate.
The mass fraction of the glutaraldehyde is 48%.
Embodiment 2
A kind of preservation liquid of sample, is mixed by weight ratio by following raw material：
4 parts of potassium sorbate, 6 parts of industrial alcohol, 2 parts of Laurocapram, 2 parts of ethylenediamine tetraacetic acid disodium zinc salt salt, protein-crosslinking agent 1
Part, 1.5 parts of bleeding agent, 2 parts of glycerine, 1.5 parts of glutaraldehyde, 8 parts of sodium chloride, 10 parts of citric acid, 90 parts of deionized water.
The protein-crosslinking agent is the composition of glyoxal, butanedial.
The bleeding agent is the composition of sodium nitrate, potassium chloride.
The mass fraction of the glutaraldehyde is 50%.
Embodiment 3
A kind of preservation liquid of sample, is mixed by weight ratio by following raw material：
5 parts of potassium sorbate, 7 parts of industrial alcohol, 3 parts of Laurocapram, 3 parts of ethylenediamine tetraacetic acid disodium zinc salt salt, protein-crosslinking agent
1.2 parts, 2 parts of bleeding agent, 3 parts of glycerine, 2 parts of glutaraldehyde, 9 parts of sodium chloride, 12 parts of citric acid, 100 parts of deionized water.
The protein-crosslinking agent be malonaldehyde, butanedial composition.
The bleeding agent is the composition of sodium chloride, potassium chloride.
The mass fraction of the glutaraldehyde is 52%.
The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be said that
The specific implementation of the present invention is confined to these explanations.For those of ordinary skill in the art to which the present invention belongs, exist
Under the premise of not departing from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to the present invention's
Protection domain.
Claims (7)
1. a kind of preservation liquid of sample, it is characterized in that：It is mixed by weight ratio by following raw material：
3-5 parts of potassium sorbate, 5-7 parts of industrial alcohol, 1-3 parts of Laurocapram, 1-3 parts of ethylenediamine tetraacetic acid disodium zinc salt salt, egg
White 0.8-1.2 parts of crosslinking agent, 1-2 parts of bleeding agent, 1-3 parts of glycerine, 1-2 parts of glutaraldehyde, 7-9 parts of sodium chloride, 8-12 parts of citric acid,
80-100 parts of deionized water.
2. the preservation liquid of sample according to claim 1, it is characterized in that：System is mixed by following raw material by weight ratio
At：
3 parts of potassium sorbate, 5 parts of industrial alcohol, 1 part of Laurocapram, 1 part of ethylenediamine tetraacetic acid disodium zinc salt salt, protein-crosslinking agent
0.8 part, 1 part of bleeding agent, 1 part of glycerine, 1 part of glutaraldehyde, 7 parts of sodium chloride, 8 parts of citric acid, 80 parts of deionized water.
3. the preservation liquid of sample according to claim 1, it is characterized in that：System is mixed by following raw material by weight ratio
At：
4 parts of potassium sorbate, 6 parts of industrial alcohol, 2 parts of Laurocapram, 2 parts of ethylenediamine tetraacetic acid disodium zinc salt salt, protein-crosslinking agent 1
Part, 1.5 parts of bleeding agent, 2 parts of glycerine, 1.5 parts of glutaraldehyde, 8 parts of sodium chloride, 10 parts of citric acid, 90 parts of deionized water.
4. the preservation liquid of sample according to claim 1, it is characterized in that：System is mixed by following raw material by weight ratio
At：
5 parts of potassium sorbate, 7 parts of industrial alcohol, 3 parts of Laurocapram, 3 parts of ethylenediamine tetraacetic acid disodium zinc salt salt, protein-crosslinking agent
1.2 parts, 2 parts of bleeding agent, 3 parts of glycerine, 2 parts of glutaraldehyde, 9 parts of sodium chloride, 12 parts of citric acid, 100 parts of deionized water.
5. the preservation liquid of sample according to claim 1, it is characterized in that：The protein-crosslinking agent be malonaldehyde, glyoxal,
One or more of butanedial composition.
6. the preservation liquid of sample according to claim 1, it is characterized in that：The bleeding agent is sodium chloride, sodium nitrate, chlorination
One or more of potassium composition.
7. the preservation liquid of sample according to claim 1, it is characterized in that：The mass fraction of the glutaraldehyde is 48-52%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810307048.5A CN108432740A (en) | 2018-04-08 | 2018-04-08 | A kind of preservation liquid of sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810307048.5A CN108432740A (en) | 2018-04-08 | 2018-04-08 | A kind of preservation liquid of sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108432740A true CN108432740A (en) | 2018-08-24 |
Family
ID=63198730
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810307048.5A Withdrawn CN108432740A (en) | 2018-04-08 | 2018-04-08 | A kind of preservation liquid of sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108432740A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109169634A (en) * | 2018-10-11 | 2019-01-11 | 内蒙古科技大学包头医学院 | The preservation liquid of human anatomy internal organ sample |
| CN110604127A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly biological specimen preserving fluid |
| CN111060374A (en) * | 2019-12-31 | 2020-04-24 | 宁夏大学 | GN aldehyde-free rapid fixing liquid and preparation method thereof |
| CN111280161A (en) * | 2018-12-06 | 2020-06-16 | 西安培华学院 | Biological specimen preserving fluid and preparation method thereof |
| CN115868475A (en) * | 2022-11-16 | 2023-03-31 | 江苏海洋大学 | Marine animal specimen preserving fluid and preparation method thereof |
-
2018
- 2018-04-08 CN CN201810307048.5A patent/CN108432740A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110604127A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly biological specimen preserving fluid |
| CN109169634A (en) * | 2018-10-11 | 2019-01-11 | 内蒙古科技大学包头医学院 | The preservation liquid of human anatomy internal organ sample |
| CN111280161A (en) * | 2018-12-06 | 2020-06-16 | 西安培华学院 | Biological specimen preserving fluid and preparation method thereof |
| CN111060374A (en) * | 2019-12-31 | 2020-04-24 | 宁夏大学 | GN aldehyde-free rapid fixing liquid and preparation method thereof |
| CN115868475A (en) * | 2022-11-16 | 2023-03-31 | 江苏海洋大学 | Marine animal specimen preserving fluid and preparation method thereof |
| CN115868475B (en) * | 2022-11-16 | 2024-02-23 | 江苏海洋大学 | Marine animal specimen preservation solution and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108432740A (en) | A kind of preservation liquid of sample | |
| Aiemsaard et al. | The effect of lemongrass oil and its major components on clinical isolate mastitis pathogens and their mechanisms of action on Staphylococcus aureus DMST 4745 | |
| CN104365581B (en) | A kind of Novel specimen preserves liquid | |
| CN106973888A (en) | A kind of preservation liquid of pathological tissue specimen | |
| JPH06507403A (en) | Glycine anhydride dimethylol as biocide and preservative | |
| JPH05271073A (en) | Anti (pseudomonas aeruginosa) agent | |
| CN107582459B (en) | A kind of preservative and preparation method thereof containing Fructus Forsythiae | |
| CN105360108A (en) | Specimen preservation fluid good in preservation effect and preparation method of specimen preservation fluid | |
| CN102754668A (en) | Novel Chinese herbal medicine compound disinfectant solution | |
| TWI538682B (en) | The use of the extract toon | |
| CN103609657A (en) | Natural preservative for fresh fishes | |
| CN111407682B (en) | Cosmetic preservative and preparation method and application thereof | |
| CN106818713A (en) | A kind of guarantor color impregnating fluid of pathological tissue specimen | |
| Gupta et al. | A study on antimicrobial activities of essential oils of different cultivars of lemongrass (Cymbopogon flexuosus) | |
| CN100534302C (en) | Preservative and its preparation method | |
| CN104970050B (en) | A kind of protease-nanometer silver complex and its application | |
| CN105340875B (en) | A kind of specimen preserving liquid and preparation method thereof | |
| CN106359367A (en) | Formaldehyde-free specimen tissue preserving fluid and preparation method thereof | |
| CN111296407A (en) | Environment-friendly composite mildew-proof preservative and application thereof | |
| CN104207956A (en) | Compound antiseptic containing natamycin and application of compound antiseptic | |
| CN111296408A (en) | Specimen environment-friendly composite mildew-proof preservative of natural plant extract and application thereof | |
| CN101810161A (en) | Environment friendly preservation solution for animal specimens | |
| CN104886165A (en) | Compound air disinfectant and preparation method thereof | |
| CN111328811B (en) | Low-concentration alcohol sterilization disinfectant and application thereof | |
| CN114376933A (en) | Antimicrobial composition for infant hand and mouth wet tissue and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180824 |