CN101810161A - Environment friendly preservation solution for animal specimens - Google Patents
Environment friendly preservation solution for animal specimens Download PDFInfo
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- CN101810161A CN101810161A CN201010172882A CN201010172882A CN101810161A CN 101810161 A CN101810161 A CN 101810161A CN 201010172882 A CN201010172882 A CN 201010172882A CN 201010172882 A CN201010172882 A CN 201010172882A CN 101810161 A CN101810161 A CN 101810161A
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Abstract
The invention relates to environment friendly preservation solution for animal specimens, belonging to the field of advanced environment friendly materials. The preservation solution comprises the following components by mass ratio: 10% to 30% of poly-hydroxymethyl heteroatomic compound and 70% to 90% of buffer solution with the pH value thereof being 4.3 to 6.5, the poly-hydroxymethyl heteroatomic compound, as a poly-functional active macromolecular compound, is capable of achieving the moderate effect of fixing animal specimens, and the buffer solution with the pH value thereof being 4.3 to 6.5 is capable of providing a weakly acidic environment for preserving the animal specimens, thus better inhibiting bacteria and mould from breeding. The preservation solution of the invention can achieve the good effects of preservation, fixation, sterilization and mould prevention on animal specimens; moreover, the preservation solution is prepared from low-toxicity environment-friendly chemicals, thus reducing the harm to human health and environment. Accordingly, the preservation solution of the invention provides an ideal substitute for formalin.
Description
Technical field
The present invention relates to a kind of environment friendly preservation solution for animal specimens, belong to the environmental friendly material field.
Background technology
The 17th century, the Egyptian has invented the chemical preservation store method of corpse.1868, Huffman (August Wilhelm vonHofmann) was found formalin solution (37% formalin) is expelled to the corruption that can prevent corpse in the blood vessel of human body in the laboratory.To 20 beginnings of the century, various chemicalss are used to the anticorrosion of corpse, and the corpus antiseptic of using till today mainly contains formalin, carbolic acid, Sodium Benzoate, glycerine and alcohol, and wherein, formalin uses the most extensive.Traditional formalin sample is preserved liquid, and good fixation is arranged, and this is because formaldehyde combines with the functional group of sample protein is a large amount of, and the further again polymerization of long-term placement forms paraformaldehyde.This process makes it to have formed the crosslinked of appropriateness at the protein peptide interchain, can sterilization, can keep the tissue morphology of sample again.But, formaldehyde, benzene class are defined as carcinogen by the World Health Organization, be listed in high malicious chemicals, meeting intense stimulus eyes, skin, respiratory mucosa etc., finally cause immunologic dysfunction, hepatic injury and nerve center system to be affected, will cause diseases such as fetal anomaly, leukemia, chronic respiratory disease, nasopharyngeal carcinoma, women's paramenia, acute depression.According to GB/T16127-1995 " sanitary standard of formaldehyde in the indoor air ", occupy the formaldehyde in indoor air maximum permissible concentration and be defined as 0.08mg/m
3And the room and the anatomy laboratory of preserving the human body dissec, the content of formaldehyde severe overweight.In 24 square metres room, the formalin with 4% is soaked sample, and air formaldehyde concentration is 1.174mg/m after 10 days
3As time goes on, air formaldehyde concentration also constantly raises, and medical science, biology and zoology worker's health has been caused serious threat.The eye stimulus threshold of gaseous formaldehyde is 0.06mg/m
3, the olfactory stimulation threshold is 0.06～0.22mg/m
3, upper respiratory tract stimulus threshold is 0.12mg/m
3, long-term chronic suction 0.45mg/m
3Formaldehyde, can cause chronic respiratory disease.This shows that the medical education worker's that anatomy is adjacent with it working environment is badly in need of improving.In addition, through the sample of formalin long preservation, can harden, the xanthochromia phenomenon, simultaneously adularescent sediment and turbid phenomenon.Formalin also can reduce because of the volatilization concentration of formaldehyde, progressively loses the function of preserving sample.
The formalin that people progressively recognize is preserved the harm of sample.In 20 end of the centurys, some methods of eliminating the formaldehyde penetrating odor begin to occur.Anatomy teaching and research room of Medical College and anatomy teaching and research room of No.1 Military Medical Univ. discover, utilize ammoniacal liquor and formaldehyde to mix the back and generate urotropine, become the principle of the water-soluble white crystal that has no irritating odor, traditional formalin store method is improved, can effectively improve the smell of preserving liquid stimulates, but has reduced stationarity, the mildew resistance of formalin simultaneously.Luzhou Medical College human dissection teaching and research room discovers, the formaldehyde volatility is bigger in the tradition preservation liquid, preserve oily matters such as adding glycerine or three pears glycan alcohol in the liquid to tradition, though have the effect of certain sealing liquid level, but the concentration of formaldehyde is still very high in the air, and the effect that suppresses volatilization is not very desirable.Yet after tradition was preserved the alkyl glyceryl ether sulfonate of adding 1% in the liquid, the volatility of formaldehyde had obviously obtained inhibition, and the concentration of preserving formaldehyde in the liquid is also more stable, is a kind of comparatively desirable processing method.But, more than two kinds of methods all cure the symptoms, not the disease, can not eradicate the influence that formaldehyde brings.
Meanwhile, the substitute products of some formalins also occur in succession.As benzoic acid-ethanol-potassium nitrate solution, concentrated seawater saturated common salt solution, surfactant solution, mould enemy's solution etc.
The research that benzoic acid-ethanol-potassium nitrate solution is preserved sample begins early.Dalian doctor high commissioner, dissection teaching and research room of Hunan College of Traditional Chinese Medicine, Hunan Medical University, anatomy teaching and research room of Ningxia Medicine College, anatomy teaching and research room of Dalian Medical Univ, changde, hunan province are defended units such as school, preclinical medicine institute of Gannan Medical College, dissection teaching and research room of No.1 Military Medical Univ. and have been carried out the research of this aspect successively.Benzoic main effect is a fungus killing, plays mold resistance; The sterilization of ethanol energy, dehydration; Potassium nitrate can improve penetration, strengthens anticorrosion mold resistance and sample is had the effect of the look of guarantor; In addition, some damp-heat area adds a small amount of formalin in addition in order to reach better anticorrosion fixation.Being used of multicomponent chemical product can the better preserve sample.Sample color and luster through this preservation liquid preservation is natural, quality is soft, hygroscopicity is strong and do not have mouldy phenomenon, and preservation liquid is as clear as crystal always, nothing precipitates, and with the sample effect no significant difference of traditional formalin preservation, is a kind of comparatively ideal sample preservation liquid in a short time.But also there are many shortcomings in this preservation liquid, ruptures easily through sample drying, the muscle fibre of its preservation.Because the volatility of ethanol, the meeting of preserving liquid was lost efficacy gradually, and particularly in the area of sweltering heat, this preservation liquid is difficult to promote.Benzoic in addition solvability is lower, and solvability only is 0.27g in 18 ℃ 100g water, can not reach anti-mold effect by increasing benzoic consumption.In addition, this sample is preserved liquid and is used jointly as not being equipped with a small amount of formaldehyde, and its fixation does not reach the requirement that sample is preserved yet, and tissue adhesion can take place the sample of long preservation.Therefore, in research from now on, need improve the recipe ingredient of benzoic acid-ethanol-potassium nitrate solution, make it to reach ideal effect.
The medical worker of biology department of Liaoning Normal University furthers investigate concentrated seawater and saturated nacl aqueous solution preservation sample.This solution is to utilize high salinity to make cell dehydration and then reach the purpose of preserving sample, and low price, nontoxic, advantage such as the source is abundant are arranged.But its performance of preserving sample is general, the hardening of sample quality, and shortcoming such as poor permeability has restricted its popularization in the cardinal principle sample.
Dissect units such as teaching and research room, Xian Medical Univ, dissection teaching and research room of Ningxia Medicine College and utilize mould enemy (1,2-benzisothiazole ketone) solution that sample is preserved experiment the Xi'an Physical Culture Institute.Mould enemy is a kind of new and effective anticorrosion anti-removing mildew; tasteless, pH value neutrality that micro white solid, nothing are smelt; aqueous solution water white transparency; dissolved degree of separating is 0.025%; now be used for fields such as leather, weaving, archives conservation, mould, bacterium, actinomycetes three major types representative strain have all been had obvious inhibitory action.Behind the preservation liquid of mould enemy's instead of formaldehyde, find that it has the effect that prevents that sample from take place rotting and going mouldy equally, to the outward appearance and the interior tissue not damaged of sample, quality is softer, color is whiter, helps dissecting operational observations as corpse, sample; This preserves, and liquid should not volatilize, non-stimulated, avirulence, makes that the concentration of formaldehyde significantly reduces in the air.But this fixation of preserving liquid is poor slightly, and mildew-proof function is poor, active ingredient 1, and the solvability of 2-benzisothiazole ketone is lower, can not be by increasing the effect that active ingredient dosage reaches fungus-proof antisepsis at damp-heat area.
Owing to lack effective environment friendly corrosion protection liquid, in the high countries and regions of environmental protection standard,, have to eliminate formalin as medical college of Hong Kong University and some national medical colleges of Southeast Asia, adopt the bigger freezing of energy consumption to preserve human body specimen.
In sum, the improvement of current sample preservation liquid mainly relies on biology, medical worker's experience accumulation, existing disinfectant is used for sample preserves.They only pay attention to the fungus and mildew resistance effect of chemicals, have ignored its fixation, though cause the phenomenon of sample softness tissue morphology fixed difference, long preservation sample even stickness.Because of often having the materialogy scientific research worker of specialty, this field do not get involved, the research of the preservation liquid of zoological specimens is a field of easily being ignored by people in addition, this utilizes formalin to preserve sample with regard to causing still having at present 90% scientific research institution, does not have a kind of preservation liquid successful, environmental protection thoroughly to substitute formalin as yet and preserves liquid.
Summary of the invention
The invention is intended to utilize the fixation coordinated of sterilization, mildew-proof function and the environmental protection low toxicity crosslinking agent of acidic buffer environment, reach the purpose of preserving zoological specimens, replace formalin and preserve liquid.
Environment friendly preservation solution for animal specimens disclosed by the invention is the heteroatomic compound of methylol more than 10%～30%, 70%～90%pH4.3～6.5 buffer solution by mass ratio.
Many methylols heteroatomic compound molecular structural formula of the present invention is: [X
+(CH
2OH)
m]
nY.Wherein, X is N, P or Si, and Y is Cl
-, NO
3 -Or SO
4 2-, m=2～4, n=1～2.
PH4.3 of the present invention～6.5 buffer solution are by sodium hydroxide (NaOH), citric acid (C
6H
8O
7H
2O), concentrated hydrochloric acid (HCl) and deionized water form, wherein the sodium hydroxide mass fraction is 0.83%～1.86%, the citric acid mass fraction is 2.10%～2.85%, the hydrochloric acid volume fraction is 0.45%～1.26%, all the other are deionized water.
Many methylols heteroatomic compound is a kind of multi-functional living polymer compound that contains, contain at least 2 methylols, heteroatomic high electronegativity has increased the activity of the methylol that is adjacent, can carry out the crosslinked of appropriateness to animal tissue's albumen, and then play certain fixation, keep the original form of zoological specimens.The mean molecule quantity of this compound is 200～800, has good hydrophily and tissue permeability.Carrying out the process that zoological specimens are preserved, can permeate at organization internal evenly, fast and have an effect.This compound is non-volatile, thereby in zoological specimens preservation process, working substance concentration does not reduce, and has overcome because of the formaldehyde volatilization shortcomings such as harm environment that cause that preservation effect is poor, air formaldehyde concentration exceeds standard.This toxicity of compound is lower, and therefore the inanimate object accumulation can not pollute environment, is the chemicals of a kind of safety, environmental protection.
As everyone knows, vinegar generally all has the shelf-life in 2～5 years.This can prove absolutely that under acid condition, microorganism should not grow.The present invention utilizes sodium hydroxide, citric acid and hydrochloric acid to be mixed with pH4.3～6.5 buffer systems, and promptly weak acid environment can well suppress the growth of bacterium, mould, guarantees that animal tissue specimens is not corroded in the process of preserving.Above-mentioned three kinds of materials are the conventional chemical product, can not pollute human body and environment.
The present invention preserves the liquid characteristics colourless transparent liquid; Have no irritating odor; The fungus and mildew resistance excellent performance; Having anti-thrombotic substance forms and the thrombus dissolving effect; Constituent does not contain volatile materials, and effect is lasting; Constituent does not contain harmful substances such as formaldehyde and aromatic compound, environmental protection.Through the animal tissue specimens that product of the present invention is preserved, that form keeps is good, soft flexible, do not go mouldy, do not corrode.Still good through the specimen quality in 5 years of this preservation liquid preservation.
Embodiment
Below by embodiment the present invention is carried out concrete description, the person skilled in the art in this field can make some nonessential improvement and adjustment according to the foregoing invention content.
Embodiment 1
With quality is many methylols of 10Kg heteroatomic compound [N
+(CH
2OH)
2] Cl
-Be dissolved in 90Kg pH4.3 buffer solution, wherein buffer solution is that 747g sodium hydroxide, 1890g citric acid and 405mL concentrated hydrochloric acid are made to 90Kg with the deionized water dissolved dilution.Finally obtain the environment friendly preservation solution for animal specimens 100Kg of pH4.3, be applicable to and preserve better fixing but the animal tissue specimens of going mouldy is arranged.
Embodiment 2
With quality is many methylols of 15Kg heteroatomic compound [P
+(CH
2OH)
4] NO
3 -Be dissolved in 85Kg pH5.3 buffer solution, wherein buffer solution is that 1224g sodium hydroxide, 2083g citric acid and 578mL concentrated hydrochloric acid are made to 85Kg with the deionized water dissolved dilution.Finally obtain the environment friendly preservation solution for animal specimens 100Kg of pH5.3, be applicable to and preserve better fixing but the animal tissue specimens of going mouldy is arranged.
Embodiment 3
With quality is many methylols of 20Kg heteroatomic compound [Si
+(CH
2OH)
3]
2SO
4 2-Be dissolved in 80Kg pH5.8 buffer solution, wherein buffer solution is that 1488g sodium hydroxide, 2280g citric acid and 840mL concentrated hydrochloric acid are made to 80Kg with the deionized water dissolved dilution.Finally obtain the environment friendly preservation solution for animal specimens 100Kg of pH5.8, be applicable to preserve and fix animal tissue specimens relatively poor but that do not go mouldy.
Embodiment 4
With quality is many methylols of 10Kg heteroatomic compound [N
+(CH
2OH)
4]
2SO
4 2-Be dissolved in 90Kg pH6.5 buffer solution, wherein buffer solution is that 1404g sodium hydroxide, 2394g citric acid and 1134mL concentrated hydrochloric acid are made to 90Kg with the deionized water dissolved dilution.Finally obtain the environment friendly preservation solution for animal specimens 100Kg of pH6.5, be applicable to and preserve animal tissue specimens better fixing and for going mouldy.
Embodiment 5
With quality is many methylols of 30Kg heteroatomic compound [P
+(CH
2OH)
3] Cl
-Be dissolved in 70Kg pH4.3 buffer solution, wherein buffer solution is that 581g sodium hydroxide, 1477g citric acid and 315mL concentrated hydrochloric acid are made to 70Kg with the deionized water dissolved dilution.Finally obtain the environment friendly preservation solution for animal specimens 100Kg of pH4.3, be applicable to preserve and fix animal tissue specimens relatively poor and that go mouldy.
Claims (3)
1. environment friendly preservation solution for animal specimens is characterized in that this preservation liquid is that many methylols heteroatomic compound of 10%～30% and pH4.3～6.5 buffer solution of 70%～90% are formed by mass ratio.
2. preservation liquid according to claim 1 is characterized in that wherein said many methylols heteroatomic compound molecular structural formula is: [X
+(CH
2OH)
m]
nY.Wherein, X is N, P or Si, and Y is Cl
-, NO
3 -Or SO
4 2-, m=2～4, n=1～2.
3. preservation liquid according to claim 1 is characterized in that wherein said pH4.3～6.5 buffer solution are by sodium hydroxide (NaOH), citric acid (C
6H
8O
7H
2O), concentrated hydrochloric acid (HCl) and deionized water form, wherein the sodium hydroxide mass fraction is 0.83%～1.86%, the citric acid mass fraction is 2.10%～2.85%, the concentrated hydrochloric acid volume fraction is 0.45%～1.26%, all the other are deionized water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010172882A CN101810161A (en) | 2010-05-12 | 2010-05-12 | Environment friendly preservation solution for animal specimens |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010172882A CN101810161A (en) | 2010-05-12 | 2010-05-12 | Environment friendly preservation solution for animal specimens |
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| Publication Number | Publication Date |
|---|---|
| CN101810161A true CN101810161A (en) | 2010-08-25 |
Family
ID=42617654
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010172882A Pending CN101810161A (en) | 2010-05-12 | 2010-05-12 | Environment friendly preservation solution for animal specimens |
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| Country | Link |
|---|---|
| CN (1) | CN101810161A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175496A (en) * | 2011-01-21 | 2011-09-07 | 哈尔滨格林标本技术开发有限公司 | Formaldehyde-free fixative for tissue sample |
| CN110604126A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly pathological tissue fixing agent |
| CN110604127A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly biological specimen preserving fluid |
| CN111296407A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Environment-friendly composite mildew-proof preservative and application thereof |
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| CN1278304A (en) * | 1997-10-30 | 2000-12-27 | 罗迪亚消费特殊有限公司 | Tanning leather |
| WO2001009392A1 (en) * | 1999-07-29 | 2001-02-08 | Rhodia Consumer Specialties Limited | Leather tanning |
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-
2010
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Patent Citations (4)
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| CN1278304A (en) * | 1997-10-30 | 2000-12-27 | 罗迪亚消费特殊有限公司 | Tanning leather |
| CN1313728A (en) * | 1998-07-22 | 2001-09-19 | 卡尔贡公司 | Co-antimicroorgan composition of peroxide acetic acid and phosphorous compounds |
| WO2001009392A1 (en) * | 1999-07-29 | 2001-02-08 | Rhodia Consumer Specialties Limited | Leather tanning |
| US20020111532A1 (en) * | 2000-12-19 | 2002-08-15 | Pathak Chandrashekhar P. | Tris(hydroxymethyl)phosphino compounds as tissue crosslinking agents |
Non-Patent Citations (2)
| Title |
|---|
| BENJAMIN L. VAN DUUREN 等: "Mouse Skin Carcinogenicity Tests of the Flame Retardants Tris(2,3-dibromopropyl)phosphate, Tetrakis(hydroxymethyl)phosphonium Chloride, and Polyvinyl Bromide", 《CANCER RESEARCH》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175496A (en) * | 2011-01-21 | 2011-09-07 | 哈尔滨格林标本技术开发有限公司 | Formaldehyde-free fixative for tissue sample |
| CN102175496B (en) * | 2011-01-21 | 2013-04-03 | 哈尔滨格林标本技术开发有限公司 | Formaldehyde-free fixative for tissue sample |
| CN110604126A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly pathological tissue fixing agent |
| CN110604127A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly biological specimen preserving fluid |
| CN111296407A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Environment-friendly composite mildew-proof preservative and application thereof |
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Application publication date: 20100825 |