KR101575603B1 - Pharmaceutical composition having antibacterial and antifungal activity and Ladies genital area cleanser composition containing the pharmaceutical composition for preventing and treating vaginitis - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for preventing and treating vaginitis which includes Lespesdeza cuneata, shoots of Agastache rugosa, pine leaves, castor oil, vinegar and desalinated deep ocean water and has antibacterial and antifungal effects. The pharmaceutical composition for preventing and treating vaginitis has excellent antibacterial activity against Candida albicans and Gardnerella vaginalis which are pathogens for vaginitis, has an effect of removing malodor, has less risk of causing side effects as the composition is made of natural materials, is not toxic to skin, does not cause an allergic reaction, and thus can be advantageously used to prevent and treat vaginitis.

Description

TECHNICAL FIELD The present invention relates to an antimicrobial composition for preventing or treating vaginitis and a method for preparing the same.

The present invention relates to a pharmaceutical composition for the prevention or treatment of vaginitis, which exhibits antibacterial and antifungal effects on Candida albicans and Gardnerella vaginalis , which are fungi, Candida albicans and general bacteria, The present invention relates to an economic composition for women,

The environment inside the vagina is very humid, warm and not affected by light, so it is a very good condition for the growth of bacteria. Therefore, normal vagina inside is formed with bacterial flora that maintains equilibrium of various microorganisms. Especially, double lactic acid bacteria weakens the inside of vagina and inhibits growth of pathogens and molds.

However, when the antibiotic is taken or contraceptive is used, the balance of the flask is broken due to various causes such as when a device such as a tampon or a loop is used, when a vaginal detergent is excessively used, when a vaginal mucous membrane is stimulated, Opportunistic inhibition The growth of opportunistic pathogens (opportunistic pathogens) leads to the development of vaginitis.

Vaginitis is largely divided into Candida vaginitis, trichomoniasis vaginitis, and bacterial vaginitis. Candida albicans is the most common form of vaginitis caused by the fungus Candida albicans , with about 75% of women experiencing vaginal and vulvar candidiasis at least once during their life, and 45% It usually occurs more than once with two or more recurrences. The major pathogenic agent of bacterial vaginosis is known as Gardnerella vaginalis and is caused by the disappearance of lactic acid bacteria normally present in the vagina, which is also the most commonly occurring vaginitis.

In order to treat vaginitis, imidazole-based agents such as polyene-based nyststin, amphotericin B, azole-based ketoconazole, and miconazole, Chemically synthesized antibiotics such as itraconazole, fluconazole and triazole have been used frequently and have been reported to have a high rate of 70%, but about 30% have recurred within 3 months (Holmes, KK, Bacterial vaginosis and women and babies, Int. J. Gynecol. Obstet., 67 Suppl 1, S9-11 (1999)).

Such chemically synthesized antibiotics may cause further problems in subsequent treatment due to resistance, and may also cause secondary complications by killing bacterial genera including lactic acid bacteria in the vagina, which may cause additional infection.

In addition, vaginal detergents often contain povidone-iodine and surfactants for cleaning power, which are known to be effective in the prevention and treatment of candida vaginitis, trichomoniasis vaginitis and other vaginal infections. Povidone-iodine is known to increase the levels of inorganic iodine or total iodine in the vagina, which can lead to hypothyroidism, and may cause mucosal damage or dermatitis due to iodine hypersensitivity. In addition, it is difficult to expect a fundamental therapeutic effect in the case of a detergent for simply maintaining vaginal titratable acidity.

Therefore, there is a demand for a composition for prevention or treatment of vaginitis which can overcome the problems of the synthetic antibiotic and the detergent without irritation and adverse effects derived from natural products.

Korean Patent No. 899,639 discloses a composition comprising as an active ingredient an extract of Ulva pertusa , which exhibits antibacterial activity against Gardnerella vaginalis , as an active ingredient, an antimicrobial composition and a composition for treating yeast infection Korean Patent Laid-Open Publication No. 2006-0036290 discloses that a herbal medicine extract containing bamboo salt, yuzun, gosam, gold, ginkgo leaf, licorice, and chamomile has a strong sterilizing power, Korean Patent No. 1,125,758 discloses that the herbal composition for local cleansing of women comprising gosam, acne, rhubarb, peony, cynthia, lycopene, peppermint and red ginseng has antimicrobial activity Effect, convergence effect, and moisturizing effect.

In addition to the above-mentioned conventional compositions, various kinds of cleaning compositions for cleansing women are disclosed. However, in order to meet the continuous demand for commercialization of pure compositions composed of natural products free from irritation and side effects, Development of the composition is required.

Accordingly, the present inventors have found that, when a combination of a night gate, a plowed ground, a pine needle, a castor oil, a vinegar, and a deionized deep sea water is not toxic or irritant, the antimicrobial activity is superior to that of a synthetic antibiotic, And finally completed the present invention.

Korean Patent No. 899,639 Korean Patent Publication No. 2006-0036290 Korean Patent No. 899,639

It is an object of the present invention to provide a pharmaceutical composition for the prevention or treatment of vaginitis having antimicrobial and antifungal effects against vaginitis causing bacteria, a method for producing the same, and a pharmaceutical composition for preventing or treating vaginitis, .

In order to achieve the above object, the present invention provides an antimicrobial and antifungal activity against a vaginitis causing bacteria in a composition comprising a plant of the order Lespsdeza cuneata , Agastache rugosa , a pine needle leaf , a pharmacopoeial , a vinegar and a desalted deep sea water The present invention provides a pharmaceutical composition for preventing or treating vaginitis having an antibacterial and antifungal effect and a method for producing the same.

In order to achieve the above object, the present invention provides a female detergent composition comprising a pharmaceutical composition for preventing or treating vaginitis as an effective ingredient.

The composition according to the present invention exhibits excellent antibacterial activity against Candida albicans and Gardnerella vaginalis which are vaginitis causative bacteria, and is weakly acidic to block the harmful bacteria, thereby preventing Candida albicans and bacterial vaginitis And it is effective in preventing or treating vaginitis as well as being effective in eliminating odor. Further, since the composition according to the present invention is made of natural materials, the risk of occurrence of side effects is low, skin toxicity and allergic reaction do not occur, and there is no need to rinse separately, so that it can be widely applied as a pharmaceutical composition for preventing or treating vaginitis and as a female cleanser .

The present invention relates to a pharmaceutical composition for prevention or treatment of vaginitis with antimicrobial and antifungal effect, comprising a topical part of Lespsdeza cuneata , Agastache rugosa , pine leaves , pharmacopoeia , vinegar and desalted deep sea water.

In one aspect of the present invention, the pharmaceutical composition comprises at least 1: 0.5 to 2: 0.5 to 2: 0.1 to 0.5: 0.1 to 0.2: 1 to 1: 10 < / RTI > by weight.

In one aspect of the present invention, the vinegar may be rice wine vinegar.

In one embodiment of the present invention, the desalted deep sea water is obtained by desalination of a deep sea water through a desalting treatment method well known in the art. Specifically, the deep sea water collected is treated with a cationic or an anionic ion exchange resin Or by desalting by performing an electrolytic process for decomposing the sodium chloride component through an electrolytic device of this type (two-bath type), desalting by a membrane filtration process, or the like, followed by desalting treatment Lt; / RTI >

In one embodiment of the present invention, the deep ocean water includes an inorganic component having an anticancer activity such as sodium (Na), calcium (Ca), magnesium (Mg), potassium (K), selenium (Se) (Fe), manganese (Mn), and the like.

In one aspect of the present invention, the deep seawater is deep seawater taken at about 300 to 1300 m, specifically about 500 to 1100 m from the east coast, west coast or south coast of Korea, preferably East Sea, Deep water (605 m) or Waterbis deep sea water (1032 m).

In one embodiment of the present invention, the deep ocean water can be desalted through a desalination process well known in the art. Specifically, deep sea water taken out is desalted using a cationic or anionic ion exchange resin Desalting treatment may be carried out by carrying out an electrolytic process in which a sodium chloride component is decomposed through an electrolytic device of the two-tank type, desalting by a membrane filtration process, or the like.

In one embodiment of the present invention, the pharmaceutical composition may exhibit antifungal and antibacterial activity against Candida albicans , a fungus, and Gardnerella Vaginalis , a common bacterium, respectively.

The present invention relates to a cleansing composition for women comprising a pharmaceutical composition for preventing or treating vaginitis having an antibacterial and antifungal effect as an active ingredient.

In one aspect of the present invention, the feminine cleansing composition may further comprise at least one additive selected from the group consisting of a surfactant, a moisturizer, a conditioning agent, a thickener, a neutralizer, a pigment and a fragrance.

In one aspect of the present invention, the feminine cleaner composition may be one for preventing or ameliorating candida vaginitis and bacterial vaginitis.

In one embodiment of the present invention, the composition for preventing or improving Candida albicans and bacterial vaginitis may be a cleanser composition, a cosmetic composition, or a wet tissue composition.

In one aspect of the invention, the cleaning may be selected from the group consisting of spray, solid, liquid, gel-type soap and foam-type, but is not limited thereto.

In one aspect of the present invention, the cosmetic composition may be selected from the group consisting of lotion, lotion, cream, essence, powder, aerosol, oil, and mist, but is not limited thereto.

The present invention also relates to a method for the production of (a) a step of drying and then plowing the ground portion of pine needles ( Lespsdeza cuneata ), Agastache rugosa and pine needles; (b) adding and stirring castor oil and vinegar; And (c) adding a deionized deep sea water and stirring the mixture. The present invention also relates to a method for producing a pharmaceutical composition for preventing or treating vaginitis having an antifungal effect.

In one embodiment of the present invention, the amount of the above-mentioned gazebo door, the above-ground portion of the pine needle, pine needles, castor oil, vinegar and desalted deep sea water is 1: 0.5 to 2: 0.5 to 2: 0.1 to 0.5: 0.1 to 0.2: .

Hereinafter, the present invention will be described in more detail with reference to the following examples and experimental examples. It should be understood, however, that the same is by way of illustration and example only and is not intended to limit the scope of the present invention.

[Example]

After washing thoroughly on the ground of Lespsdeza cuneata , Agastache rugosa , and the water flowing through the pine needle, it was drained by wind. And then dried in a cool shade for 3 days. A well-dried nightclub, weighing pan, and pine needle were weighed and weighed 400 g each to a particle size of 5-10 mm. Thereafter, it was further placed in a grinder, and then further sieved with a 200-mesh sieve. To this, 40 mL of castor oil and 40 mL of makgeolli vinegar were added and stirred to obtain a slurry-like mixture. To this was added 1 L of deionized deep sea water (deep sea marine deep sea water) and the mixture was stirred at high speed for 1 hour to obtain a composition of Example.

The added makkolli vinegar was prepared by pouring raw makkolli (uncooked rice wine from Chiaksan) into a cleanly disinfected glass container, fixing it with a broad bean sprout, fixing it with a rubber band, and fermenting it by leaving it in a shade for 60 days.

In addition, the desalted deep-sea water was used as deep-sea water of Watersbis Co., Ltd. (manufactured by desalting at a depth of 1032 m at a distance of 17.5 km from Yangyang Sea in Gangwon Province).

[Experimental Example]

Experimental Example 1. Antimicrobial activity measurement by disk diffusion method

The pre-cultured Candida albicans and Gardnerella vaginalis were each plated on a plate medium (Sabouraud Dextrose Agar Difco. USA) for measurement of disk diffusion method using platinum at a rate of 100 μL, and then the composition of Example was sterilized The sample was placed on a paper disk at a concentration of 0.5 mg / disc. In the evaluation of antimicrobial activity against Candida albicans, itraconazole was used as a positive control, and metronidazole was used as a positive control in evaluating the antimicrobial activity against Gardnerella var. Nalis. In the case of itraconazole, a concentration of 100 μg / The sample was loaded at a concentration of 50 μg / disc and placed on the medium. This plate culture medium was cultured at 28 ° C for Candida albicans and 37 ° C for Gardnerella vaginalis, and the size of the growth inhibition circle was measured.

As a result, the sizes of the growth inhibition rings of the examples according to the present invention were larger in the Candida albicans and Gardnerella vajinalis, which are the vaginitis causing bacteria, than in the positive control, which is a chemosynthesis antibiotic. Therefore, it was confirmed that the example had better antibacterial activity than the synthetic antibiotic (Table 1).

Experimental Example 2: Cytotoxicity test

Human fibroblast cells were seeded in DMEM medium containing 10% FBS at a concentration of 1 × 10 ⁵ cells / mL on a 24-well plate, and then the medium was changed after 24 hours. Then, the compositions of the examples were added at concentration (10, 25, 50, 75, 100 mg / L) and cultured in a 5% CO ₂ incubator at 37 ° C for 5 days. The MTT solution was added at a rate of 1 mL / well, followed by incubation at 37 ° C in a 5% CO ₂ incubator for 3 hours. The medium was removed, 200 μL of DMSO was added thereto, and the absorbance was measured at 550 nm.

As a result, cell growth inhibition was hardly observed at a concentration of 75 mg / L and cell viability was slightly decreased at a concentration of 100 mg / L (Table 2).

Experimental Example 3.

Eight female patients were applied to the affected area once a day for 1 week, and then the smell and the irritation of the affected area were questioned. As a result, 6 out of 8 respondents answered that they did not smell bad, and 2 out of 8 respondents said they did not know. In addition, 8 respondents answered that they did not feel the irritation of the affected person. Therefore, it can be seen that the composition according to the present invention has some effect on the removal of odor, and has no irritation to the affected area.

Claims (8)

A town hall ( Lespsdeza cuneata );
Above ground of Agastache rugosa ;
pine needles;
Pharmacy Freedom; vinegar; And
A pharmaceutical composition for the prevention or treatment of vaginitis with antibacterial and antifungal effects comprising deionized deep sea water.
The method according to claim 1,
Characterized in that the composition of the above composition is comprised in a weight ratio of 1: 0.5 to 2: 0.5 to 2: 0.1 to 0.5: 0.1 to 0.2: 1 to 10: ≪ / RTI > or a pharmaceutically acceptable salt thereof.
The method according to claim 1,
Wherein the composition has antifungal and antibacterial activity against Candida albicans , a fungus, and Gardnerella vaginalis , a common bacterium, respectively.
A feminine cleaning composition comprising a pharmaceutical composition for preventing or treating vaginitis according to any one of claims 1 to 3 as an active ingredient.
5. The method of claim 4,
Wherein the female cleaner composition further comprises at least one additive selected from the group consisting of a surfactant, a moisturizer, a conditioning agent, a thickener, a neutralizer, a pigment and a flavoring agent.
5. The method of claim 4,
Wherein said female cleaner composition is for preventing or ameliorating candida vaginitis and bacterial vaginitis.
(a) Drying and subdividing of the ground and pine needles of the insects ( Lespsdeza cuneata ), Agastache rugosa ;
(b) adding and stirring castor oil and vinegar; And
(c) adding a deionized deep sea water and stirring.
A method for the preparation of a pharmaceutical composition for the prevention or treatment of vaginitis having an antibacterial and antifungal effect.
8. The method of claim 7,
Characterized in that the amount of the above-mentioned gazebo door, the above-ground portion of the pine needle, the pine needle, the castor oil, the vinegar and the deep sea water is 1: 0.5~2: 0.5~2: 0.1-0.5: 0.1~0.2~1-10 weight ratio. A method for preparing a pharmaceutical composition for therapeutic use.