KR20200031404A - Feminine Cleanser Composition With Phyto-Chitosan, Method for Preparing the Same and Feminine Cleanser Comprising the Same - Google Patents

Abstract

A feminine cleanser composition according to the present invention contains, with respect to 100 parts by weight of a polar solvent, 0.01 to 0.1 parts by weight of vegetable chitosan, 0.05 to 0.5 parts by weight of organic acid, and a basic substance. Therefore, it is possible to provide a female cleanser exhibiting excellent antibacterial, antifungal, deodorizing, and moisturizing properties.

Description

Feminine Cleanser Composition With Phyto-Chitosan, Method for Preparing the Same and Feminine Cleanser Comprising the Same}

The present invention relates to a feminine cleanser composition showing excellent antibacterial, antifungal, deodorizing, and moisturizing properties using vegetable chitosan, a method for manufacturing the same, and a feminine cleanser comprising the same.

Inside the woman's vagina, many lactic acid bacteria inhabit and maintain a weakly acidic state, which inhibits the growth of pathogens and fungi. For example, Lactobacillus rhamnosus present in the vagina has an antibiotic effect against E. coli and Staphylococcus aureus, and serves to keep organs of the genital system healthy, and Lactobacillus fermentum has a strong antibacterial effect against Salmonella and E. coli, and gynecological diseases. It is effective in prevention and treatment. In addition, Lactobacillus reuteri and Lactobacillus plantarum are effective against candidiasis due to their antifungal function .

However, when the immune system inside the vagina collapses due to changes in female hormones due to long-term use of antibiotics or contraceptives, pregnancy or childbirth, lactation, menopause, etc., under normal conditions, overgrowth of anaerobic bacteria and fungi that normally inhibit growth Vaginitis may occur.

Specifically, bacterial infections such as gardnerella vaginitis, chlamydia vaginitis, candida vaginitis, trichomoniasis vaginitis caused by protozoa, and atrophic vaginitis caused by menopause may occur, in which case the pH balance inside the vagina is destroyed As the growth of harmful bacteria becomes active, symptoms such as itching, burning, changes in the color and smell of vaginal secretions, and changes in the concentration of secretions appear.

In order to treat such vaginitis, it is recommended to wash the vulva and the inside of the vagina using water or other liquid mixture. In this regard, products using simple distilled water, hydrogen peroxide or povidone, natural extracts, etc. are commercially available as feminine cleaners.

However, in the case of simple distilled water, the cleaning ability is present, but the effect is insufficient, and there is no ability to prevent proliferation and infection prevention of vaginitis-causing bacteria. In addition, in the case of products using hydrogen peroxide and povidone iodine, it has a bactericidal component, so it can remove irritants that cause bacterial vaginosis, but it can be irritating to the vaginal mucosa and also remove beneficial bacteria. Can be. In addition, many products using natural extracts extracted from aloe, cactus, plantain, ginkgo biloba, ginseng, Angelica, herbs, rosemary, etc. have been released, but such as simple secretion washing and odor removal, rather than solving the underlying cause of vaginitis There is a problem in that its function is concentrated in maintaining cleanliness.

Moreover, when taking antibiotics to treat vaginitis, antibiotics can not only remove harmful bacteria in the vagina, but also beneficial bacteria, so the root cause of vaginitis cannot be solved. The risk of spreading to the fallopian tubes in the uterus increases the risk of infertility or pelvic infection, and the infection rate of various sexually transmitted diseases also increases.

Accordingly, various studies have been conducted on techniques for suppressing growth of bacteria, mold, etc., which cause vaginitis using herbal compositions. For example, in Patent Publication No. 10-2006-0036290 'Women's Cleanser Composition Containing Bamboo Salt and Complex Herbal Medicine Extract', it is for women including bamboo salt and complex herbal extracts containing yugeunpi, ginseng, golden, ginkgo biloba, licorice and chamomile The content of the cleanser composition is disclosed, and in the registered patent No. 101125758 'Women's Local Cleansing Cleanser Composition', the contents of the herbal composition for female local cleansing consisting of red ginseng, lobster, rhubarb, peony, celestial, almond, peppermint, and red ginseng can confirm. However, when using such a herbal composition, there is a problem that it is difficult to expect an immediate therapeutic effect due to the characteristics of the herbal material.

Accordingly, there is a need to develop a cleaning agent for women capable of preventing and treating vaginitis by exhibiting functions such as selective antibacterial and antifungal activity against harmful bacteria at the same time as washing excessive secretions generated in the vagina.

<Prior Art Document>

Korean Patent Publication No. 10-2006-0036290

Korean Registered Patent No. 101125758

The present invention aims to solve the problems of the prior art as described above and the technical problems requested from the past.

Specifically, an object of the present invention is to provide a feminine cleanser composition, a manufacturing method thereof, and a feminine cleanser comprising the same, which can prevent and treat vaginitis through washing inside the vagina while removing secretions remaining in the vulva.

The present invention,

It provides a cleansing composition for women using vegetable chitosan having antibacterial and antifungal effects, including 0.01 to 1 part by weight of vegetable chitosan, 0.05 to 0.5 part by weight of organic acid, and a basic substance, based on 100 parts by weight of a polar solvent.

The vegetable chitosan can be derived from mushrooms.

The molecular weight of the vegetable chitosan may be 200 to 20,000 kDa.

The polar solvent may be distilled water, methanol, ethanol, propanol, or a mixture thereof.

The organic acid may be at least one selected from the group consisting of acetic acid, tartaric acid, salicylic acid, citric acid, hydroxy acetic acid, lingoic acid, propionic acid, lactic acid, glycerin acid, ascorbic acid, adipic acid, and malic acid.

The basic substance may be one or more selected from the group consisting of sodium hydroxide, calcium hydroxide, magnesium hydroxide, and potassium hydroxide.

The composition may be for cleaning the vulva and for cleaning the vagina.

The composition for washing the vulva may be 0.03 to 0.08 parts by weight of vegetable chitosan with respect to 100 parts by weight of a polar solvent.

The composition for cleaning the vagina inside may be 0.01 to 0.03 parts by weight of vegetable chitosan, based on 100 parts by weight of a polar solvent.

In addition, the present invention provides a feminine cleaner using vegetable chitosan containing a feminine cleaner composition as an active ingredient.

The feminine cleanser, the feminine cleanser composition may be included in 60 to 100% by weight based on the total weight of the female cleanser.

The feminine cleanser may be for detergent, cosmetic, or wipes.

The cleaning agent may be formulated as a spray, liquid, gel or foam type.

The cosmetic use may be formulated as a lotion, lotion, cream, essence, powder, aerosol, oil or mist.

In addition, the present invention

As a method of manufacturing a woman cleanser composition,

(A) Step of preparing vegetable chitosan

(B) a first mixed stirring of 0.05 to 0.5 parts by weight of an organic acid with respect to 100 parts by weight of a polar solvent, followed by a second mixing and stirring of 0.01 to 1 parts by weight of vegetable chitosan; And,

(C) adjusting the pH to be 4.5 to 5.5 by adding a basic substance;

It provides a method for producing a cleaning composition for women comprising a.

The vegetable chitosan of step (a) may be prepared by subjecting the mushroom to an aqueous alkali solution, followed by separation, washing and drying.

The first mixing agitation and the second mixing agitation of step (B) may be performed at room temperature.

The first mixing and stirring of the step (B) is made for 1 to 3 minutes, and the second mixing and stirring can be performed for 20 to 80 minutes.

Women's cleansing agent using vegetable chitosan according to the present invention exhibits selective antibacterial and antifungal properties, so it can suppress the growth of harmful bacteria that cause vaginitis without killing beneficial bacteria inside the vaginal salt. It can be prevented and treated. In addition, since it can be used for washing the secretions generated in the vagina and remaining in the vulva, it has excellent deodorizing and moisturizing effects, so it can be used to maintain the quality of vagina in everyday life.

Moreover, since it is easily decomposed in the body using vegetable chitosan, residues are not left, and thus it is less likely to cause side effects, so it can be safely used as a female cleanser.

1 (a) and (b) are photographs and graphs showing the results of evaluation of the antibacterial effect of Experimental Example 1, respectively;
2 (a) and (b) are photographs and test reports showing the results of evaluation of antibacterial and antifungal effects of Experimental Example 2, respectively;
3 is a graph showing the results of evaluation of antibacterial and antifungal effects by chitosan concentration in Experimental Example 3;
4 (a) and (b) are graphs and photographs showing the results of evaluation of the survival rate of beneficial bacteria in the vagina of Experimental Example 4, respectively;
5 is a graph showing the evaluation results of the ammonia deodorization effect of Experimental Example 5;
6 is a graph showing the evaluation results of the trimethylamine deodorization effect of Experimental Example 6; And
7 (a) and (b) are graphs showing evaluation results of the ammonia and trimethylamine gas deodorization effect of Experimental Example 7, respectively.

The present invention,

It provides a cleansing composition for women using vegetable chitosan having antibacterial and antifungal effects, including 0.01 to 1 part by weight of vegetable chitosan, 0.05 to 0.5 part by weight of organic acid, and a basic substance, based on 100 parts by weight of a polar solvent.

Women cleaner composition according to the invention do not kill of yuikgyun such as Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus fermentum , Lactobacillus plantarum that live in the vagina through a selective antibacterial, antifungal action by the chitosan even vegetable, such as E.Coli By suppressing the growth of harmful bacteria, it is possible to maintain a weakly acidic environment inside the vagina. In addition, by removing excessive secretions generated in the vagina through washing the vulva, it can exhibit excellent deodorizing and moisturizing effects, and can be used to maintain cleanliness, such as vaginal rinsing and cleaning of vagina in everyday life.

The vaginitis may be, for example, candidal vaginitis caused by Candida albicans, bacterial vaginosis caused by Gardnerella vaginalis .

Vegetable chitosan in the present invention is a chitosan derived from fungi such as mushrooms, black mold, water fungi, actinomycetes, and is distinguished from animal chitosan obtained from crustaceans such as crabs and shrimp. These vegetable chitosans contain β-glucan, which has immune-enhancing and anti-cancer effects, and thus not only exhibits excellent antibacterial and antifungal properties, but also easily decomposes in the body compared to animal chitosans, leaving no residue, which is likely to cause side effects. It is low and can be used safely as a female cleanser.

The vegetable chitosan may be, in detail, mushroom chitosan derived from mushrooms. The mushrooms may be, for example, shiitake mushrooms, wood ear mushrooms, enoki mushrooms, glued mushrooms, agaricus mushrooms, but are not limited thereto.

The molecular weight of the vegetable chitosan may be 200 to 20,000 kDa. When the molecular weight of the vegetable chitosan is less than 200 kDa, it is difficult in the manufacturing process, and the actual antibacterial and antifungal effects may be lowered, which is undesirable, and when the molecular weight exceeds 20,000 kDa, it is not easily decomposed and may remain as a residue in the body. Therefore, it is not preferable because it may cause side effects such as antibody formation. Thus, in detail, the molecular weight of the vegetable chitosan may be 200 to 10,000 kDa, and more specifically 200 to 2,000 kDa.

The polar solvent may be distilled water, methanol, ethanol, propanol, or a mixture thereof. In detail, it may be distilled water, but in some cases, 0.1 to 10 parts by weight of alcohol, such as methanol, ethanol, and propanol, may be added to 100 parts by weight of distilled water to improve preservability and antibacterial activity.

The organic acid may be, for example, one or more selected from the group consisting of acetic acid, tartaric acid, salicylic acid, citric acid, hydroxy acetic acid, lingoic acid, propionic acid, lactic acid, glycerin acid, ascorbic acid, adipic acid, and malic acid, In detail, it may be ascorbic acid, which is not irritating and has an antioxidant function, which is advantageous for improving immunity.

The basic material may be, for example, one or more selected from the group consisting of sodium hydroxide, calcium hydroxide, magnesium hydroxide, and potassium hydroxide, and specifically, sodium hydroxide.

In the cleanser composition for women according to the present invention, it is difficult to obtain the desired effects in the present invention, such as antibacterial and antifungal effects, when the content of vegetable chitosan is less than 0.01 parts by weight with respect to 100 parts by weight of the polar solvent, and if it exceeds 1 part by weight, only harmful bacteria In addition, it may have a negative effect on the growth and survival of beneficial bacteria, such as lactic acid bacteria, and is not preferable because there is a fear that manufacturing costs may increase.

In addition, if the organic acid is less than 0.05 parts by weight with respect to 100 parts by weight of the polar solvent, there is a fear that the vegetable chitosan is not sufficiently dissolved, and when the organic acid exceeds 0.5 parts by weight, it acts excessively irritating to the skin, which may cause itching, itching, etc. It is not desirable.

The basic substance may be added in an appropriate amount to adjust the pH of the composition of the feminine cleanser, and may be adjusted to a pH level of 4.5 to 5.5 in the vagina to maintain a pH balance in the vagina.

In detail, the feminine cleanser composition according to the present invention may include 0.01 to 0.08 parts by weight of vegetable chitosan, 0.08 to 0.3 parts by weight of organic acid, and 0.01 to 0.10 parts by weight of basic substance with respect to 100 parts by weight of a polar solvent.

In addition, the inventors of the present invention confirmed that the composition can be used separately for vulva cleaning and vaginal internal cleaning. This, as can be seen in the following experimental examples, considering the degree of killing of harmful and beneficial bacteria according to the content of vegetable chitosan, specifically, the composition for washing the vulva is 100 parts by weight of polar solvent, 0.03 to 0.08 vegetable chitosan It may contain parts by weight, and the composition for internal vaginal cleaning may contain 0.01 to 0.03 parts by weight of vegetable chitosan with respect to 100 parts by weight of a polar solvent, and organic acids and basic substances may be appropriately adjusted within the above-defined range.

Women's cleansing agent using vegetable chitosan according to the present invention exhibits selective antibacterial and antifungal properties, so it can suppress the growth of harmful bacteria that cause vaginitis without killing beneficial bacteria inside the vaginal salt. It can be prevented and treated. In addition, since it can be used for washing the secretions generated in the vagina and remaining in the vulva, it has excellent deodorizing and moisturizing effects, so it can be used to maintain the quality of vagina in everyday life.

In addition, the present invention provides a female cleaner using vegetable chitosan containing the female cleaner composition as an active ingredient. The feminine cleanser composition may include 60 to 100% by weight based on the total weight of the feminine cleanser.

In some cases, the feminine cleanser, within a range that does not degrade the effect of the present invention, various components commonly used in external preparations for skin, for example, water-soluble components, powder components, oils, surfactants, moisturizers, viscosity modifiers Of course, it can be blended with preservatives, antioxidants, fragrances, pigments, and the like.

The female cleanser can be used in various forms, for example, it can be used for detergents, cosmetics, wipes, and the like.

The cleaning agent may be formulated as a spray type, liquid type, gel type, or foam type, and the cosmetic type may be formulated as a lotion, lotion, cream, essence, aerosol, powder, aerosol, oil or mist. have. In addition, the liquid formulation may be applied to a non-woven fabric or pulp in the form of a wet tissue.

On the other hand, the present invention,

(A) Step of preparing vegetable chitosan

(B) a first mixed stirring of 0.05 to 0.5 parts by weight of an organic acid with respect to 100 parts by weight of a polar solvent, followed by a second mixing and stirring of 0.01 to 1 parts by weight of vegetable chitosan; And,

(C) adjusting the pH to be 4.5 to 5.5 by adding a basic substance;

It provides a method for producing a cleaning composition for women comprising a.

Vegetable chitosan in step (a) can be obtained by a method known in the art from fungi such as mushrooms, black mold, water fungi, actinomycetes, and in detail, after heat treatment of an aqueous alkaline solution of mushrooms, separation and washing And dried to prepare. The mushrooms may be, for example, shiitake mushrooms, wood ear mushrooms, enoki mushrooms, glued mushrooms, agaricus mushrooms, but are not limited thereto.

The molecular weight of the vegetable chitosan may be 200 to 20,000 kDa. When the molecular weight of the vegetable chitosan is less than 200 kDa, it is difficult in the manufacturing process and the antibacterial and antifungal effects may be deteriorated, which is undesirable. When the molecular weight exceeds 20,000 kDa, it is not easily decomposed and may remain as a residue in the body. It is not preferable because it may cause side effects such as antibody formation. Thus, in detail, the molecular weight of the vegetable chitosan may be 200 to 10,000 kDa, and more specifically 200 to 2,000 kDa.

In general, since the vegetable chitosan has poor solubility in a polar solvent, particularly distilled water, it is necessary to add the organic acid first, followed by the first mixing and stirring, and then add the vegetable chitosan and the second mixing and stirring.

In this case, the first mixing and stirring may be performed at room temperature for 1 minute to 3 minutes until the organic acid is completely dissolved in the polar solvent, and the second mixing and stirring is performed at room temperature until 20 minutes at room temperature until the vegetable chitosan is completely dissolved in the organic acid. It can be done for 80 minutes. If the temperature and time conditions are exceeded, it is not preferable because the organic acid or the vegetable chitosan is not sufficiently dissolved or the manufacturing process efficiency may decrease.

With respect to 100 parts by weight of the polar solvent, when the content of vegetable chitosan is less than 0.01 parts by weight, it is difficult to obtain a desired effect in the present invention, such as washing power, antibacterial power, and antifungal power, and when it exceeds 1 part by weight, harmful bacteria as well as beneficial bacteria such as lactic acid bacteria It may have a negative effect on growth and survival, and manufacturing costs may increase, which is undesirable.

In addition, if the organic acid is less than 0.05 parts by weight with respect to 100 parts by weight of the polar solvent, there is a fear that the vegetable chitosan is not sufficiently dissolved, and when the organic acid exceeds 0.5 parts by weight, it acts excessively irritating to the skin, which may cause itching, itching, etc. It is not desirable.

Thus, in detail, with respect to 100 parts by weight of the polar solvent, the organic acid may be added with 0.08 to 0.3 parts by weight of organic acid, followed by first mixing and stirring, followed by addition of 0.02 to 0.8 parts by weight of vegetable chitosan, followed by second mixing and stirring.

After the first mixing agitation and the second mixing agitation, a basic substance may be added in an appropriate amount to adjust the pH of the composition of the feminine cleanser, and is generally adjusted to a pH level of 4.5 to 5.5 inside the vagina to adjust the inside of the vagina. It can maintain the pH balance. Specifically, 0.01 to 0.10 parts by weight of the basic substance may be added to 100 parts by weight of the polar solvent.

The polar solvent may be distilled water, methanol, ethanol, propanol, or a mixture thereof. In detail, it may be distilled water, but in some cases, 0.1 to 10 parts by weight of alcohol, such as methanol, ethanol, and propanol, may be added to 100 parts by weight of distilled water to improve storage, antibacterial and antifungal properties.

The organic acid may be, for example, one or more selected from the group consisting of acetic acid, tartaric acid, salicylic acid, citric acid, hydroxy acetic acid, lingoic acid, propionic acid, lactic acid, glycerin acid, ascorbic acid, adipic acid, and malic acid, Specifically, it may be ascorbic acid, which is advantageous for improving immunity due to its antioxidant function.

The basic material may be, for example, one or more selected from the group consisting of sodium hydroxide, calcium hydroxide, magnesium hydroxide, and potassium hydroxide, and specifically, sodium hydroxide.

Hereinafter, examples of the present invention will be described in detail. However, the following examples are only to illustrate the present invention, the content of the present invention is not limited to the following examples.

Example 1

Vegetable chitosan powder having a molecular weight of 200 to 2,000 kDa, derived from mushrooms of Beijing Wisapple Biotech, was prepared.

After mixing 0.1 g of vitamin C (ascorbic acide) powder in 100 ml of distilled water, the mixture was stirred for 2 minutes at room temperature until completely dissolved. After vitamin C was completely dissolved, 0.05 g of the vegetable chitosan powder was mixed and stirred for 1 hour at room temperature until completely dissolved.

After the vegetable chitosan was completely dissolved, 0.03 g of NaOH in a bead state was added to adjust the pH to 4.5 to prepare a female cleanser.

Examples 2 to 7

Vegetable chitosan powder 0.01 g (Example 2), 0.025 g (Example 3), 0.04 g (Example 4), 0.1 g (Example 5), 0.5 g (Example 6), and 1 g (Example), respectively. A female cleanser was prepared in the same manner as in Example 1, except that Example 7) was used.

Comparative Example 1

A control was prepared.

Comparative Example 2

As a feminine cleanser, Hyunjin Corporation's watercolor ^TM was prepared.

Comparative Example 3

As a feminine cleanser, CEPE ^TM of San-ei Pharmaceutical Co., Ltd. was prepared.

Comparative Example 4

MISUMIZU's Inner Refreshing Wash ^TM was prepared as a female cleanser.

Comparative Example 5

As a cleanser for women, Howard Angel's plantain ^TM was prepared.

Experimental Example 1

(1) Test method

After mixing the feminine cleansers of Example 1 and Comparative Examples 1 to 3 with the medium, each of the E. coli was plated and cultured at 37 ° C. for 8 to 12 hours, followed by visual observation using the E. coli colony counting method.

(2) Test results

As shown in Figure 1 (a), 1 (b) below, the female cleaner using the vegetable chitosan of Example 1 (developed product) Comparative Example 1 (control), Comparative Example 2 (watercolor), Comparative Example 3 (CEPE) ), As the proliferation of E.coli is almost 100% suppressed, it can be confirmed that the antibacterial effect is excellent.

Experimental Example 2

(1) Test method

For the antibacterial and antifungal tests for Example 1, the KS K 0693 test method was used to request a test from the Korea Research Institute of Standards and Standards (KSTR). Staphylococcus aureus, E. coli, Pseudomonas aeruginosa and Candida bacteria were used for antibacterial and antifungal tests.

* KS K 0693 Test Method: Inoculate the stock of each strain stored in a cryogenic freezer in MSR broth and incubate for 12 hours in a 37 ° C incubator. After incubation, absorbance was measured using a spectrophotometer to measure the number of viable cells in 1. After preparing the viable water in 20 ml of chitosan sample to be used for the test, (1 ± 0.3) x10 ⁵ pcs / ml was prepared, and 18 hours at 37 ° C. Incubate. After 20 ml of sterile physiological saline at 0 ° C was added to the cultured solution, the mixture was shaken vigorously, and the bacterial solution was extracted from each sample and diluted 1 to 10000 times using physiological saline. Exactly 1.0 ml of the diluted sample is picked up with a pipette, dropped into a petri dish, and then pour about 15 ml of agar medium at 45 ~ 46 ° C to mix evenly. After incubation, colonies are counted and recorded.

(2) Test results

As shown in Figure 2 (a) and 2 (b) below, the female cleaner using the vegetable chitosan of Example 1 (developed product) compared to Comparative Example 1 (control) Staphylococcus aureus, E. coli, Pseudomonas aeruginosa, Candida bacteria The proliferation of is inhibited, in particular, Example 1 (developed product) can be confirmed that it shows a 99.9% bacteriostatic reduction rate, and can be preferably used as a female cleanser.

Experimental Example 3

(1) Test method

For the antibacterial and antifungal tests for Examples 1, 3 and 4, KS K 0693 test method was used to request a test from the Korea Research Institute of Standards and Standards (KSTR). Staphylococcus aureus, E. coli, Pseudomonas aeruginosa and Candida bacteria were used for antibacterial and antifungal tests.

(2) Test results

As shown in Fig. 3, the feminine cleanser using vegetable chitosan of Example 3 (0.025%) and Example 4 (0.04%) was 99.9% against Staphylococcus aureus, E. coli, Pseudomonas aeruginosa, as in Example 1 (0.05%). It showed a bacteriostatic reduction rate of, and for Candida, it can be confirmed that the bacteriostatic reduction rate was 80.3% and 90.3%, respectively.

Experimental Example 4

(1) Test method

The survival rate (%) for Lactobacillus plantarum and Lactobacillus ruteri was measured using the female cleaners of Example 3, Comparative Example 1, and Comparative Example 5 using the KS K 0693 test method.

(2) Test results

As shown in Figures 4 (a) and 4 (b) below, the viability of Lactobacillus plantarum and Lactobacillus ruteri , useful microorganisms in the vagina, in women's cleansing agent ( plain plantain) of Comparative Example 5 is 0%, whereas in Example 3 (0.025%) Useful microorganisms in female cleansers using vegetable chitosan showed survival rates of 60% or more and 50% or more, respectively. Therefore, the feminine cleanser of Example 3 can be preferably used, especially for internal vaginal cleaning.

Experimental Example 5

 (1) Test method

Ammonia gas detector method is used. That is, the feminine cleaners of Examples 1 and 3 were added to the plastic bag filled with ammonia gas, and after 2 hours, the residual ammonia level in the plastic bag was measured and the deodorization rate was calculated using the following formula.

Deodorization rate (%) = ((Cb-Cs) / Cb) * 100

(Cb: BLANK, concentration of test gas remaining in the test gas bag after 2 hours elapsed, Cs: sample, concentration of test gas remaining in the test gas bag after 2 hours elapsed)

(2) Test results

As shown in Figure 5 below, the feminine cleanser using the vegetable chitosan of Example 1 (developed product) is generated during protein degradation compared to Comparative Example 1 (control), Comparative Example 2 (watercolor), Comparative Example 3 (CEPE). It can be seen that the ammonia to be effectively deodorized shows excellent deodorization rate (over 99%).

Experimental Example 6

 (1) Test method

Tremethylamine gas detector method is used. That is, each of the female cleaners of Examples 1 and 3 to 5 was put into a plastic bag filled with trimethylamine gas, and after 2 hours, the residual trimethylamine value in the plastic bag was measured and the deodorization rate was calculated using the following formula.

Deodorization rate (%) = ((Cb-Cs) / Cb) * 100

(Cb: BLANK, concentration of test gas remaining in the test gas bag after 2 hours elapsed, Cs: sample, concentration of test gas remaining in the test gas bag after 2 hours elapsed)

(2) Test results

As shown in Figure 6 below, the feminine cleanser using vegetable chitosan of Example 1 compared to Comparative Example 3 (CEPE), Comparative Example 4 (Misumizu), and Comparative Example 5 (Plant), the main odor of odor occurring in the vagina It can be confirmed that the detrimental efficiency of trimethylamine is effectively deodorized and the deodorization rate (40% or more) is exhibited.

Experimental Example 7

(1) Test method

The gas detection tube method of the same method as Experimental Examples 5 and 6 was used. Deodorization rates for ammonia and trimethylamine gas by chitosan concentrations for the female cleansers of Examples 1, 2, 5, 6, and 7 are measured.

(2) Test results

As shown in Figure 7 (a) below, Example 1 (0.05%), 2 (0.01%), 5 (0.1%), 6 (0.5%), and 7 (1.0%) of ammonia for the female cleanser In the case, the deodorization rate of 99% or more was exhibited in all concentration ranges tested, and in the case of trimethylamine in FIG. 7 (b), the deodorization rate also increased as the concentration of chitosan increased.

Claims (18)

Cleaner composition for women using vegetable chitosan, characterized in that it has antibacterial and antifungal effects, including 0.01 to 1 part by weight of vegetable chitosan, 0.05 to 0.5 part by weight of organic acid, and a basic substance, based on 100 parts by weight of a polar solvent. The method of claim 1, wherein the vegetable chitosan is a feminine cleanser composition using vegetable chitosan, characterized in that derived from mushrooms. The method of claim 1, wherein the molecular weight of the vegetable chitosan is a cleanser composition for women using vegetable chitosan, characterized in that 200 to 20,000 kDa. The method of claim 1, wherein the polar solvent, distilled water, methanol, ethanol, propanol, or a feminine cleaner composition using vegetable chitosan, characterized in that a mixture thereof. ` The method according to claim 1, wherein the organic acid is at least one selected from the group consisting of acetic acid, tartaric acid, salicylic acid, citric acid, hydroxy acetic acid, lingoic acid, propionic acid, lactic acid, glycerin acid, ascorbic acid, adipic acid, and malic acid. Cleanser composition for women using vegetable chitosan. The method of claim 1, wherein the basic substance, sodium hydroxide, calcium hydroxide, magnesium hydroxide, and potassium hydroxide, a feminine cleaner composition using vegetable chitosan, characterized in that at least one selected from the group consisting of. The method of claim 1, wherein the composition, for cleaning the vulva and internal vaginal cleansing composition for women using vegetable chitosan, characterized in that. According to claim 7, wherein the composition for cleaning the vulva is a cleansing composition for women using vegetable chitosan, characterized in that 0.03 to 0.08 parts by weight of vegetable chitosan with respect to 100 parts by weight of a polar solvent. According to claim 7, wherein the composition for cleaning the vagina inside, 100 parts by weight of a polar solvent, vegetable chitosan 0.01 to 0.03 parts by weight of a vegetable cleanser composition for women using vegetable chitosan. A female cleanser using vegetable chitosan, characterized in that it comprises as a active ingredient the feminine cleanser composition according to claim 1. 11. The method of claim 10, wherein the feminine detergent, feminine detergent composition for women using vegetable chitosan, characterized in that 60 to 100% by weight based on the total weight of the female detergent. 11. The method of claim 10, wherein the feminine cleanser is a feminine cleanser using vegetable chitosan, characterized in that for the detergent, cosmetic, or wipes. The cleaner for women using vegetable chitosan according to claim 12, wherein the detergent is formulated into a spray type, a liquid type, a gel type, or a foam type. The method of claim 12, wherein the cosmetic is a lotion, lotion, cream, essence, powder, aerosol, oil or mist formulated with a vegetable cleanser for women using vegetable chitosan. As a method for producing a woman cleanser composition,
(A) Step of preparing vegetable chitosan
(B) a step of first mixing and stirring 0.05 to 0.5 parts by weight of an organic acid with respect to 100 parts by weight of a polar solvent, followed by a second mixing and stirring of 0.01 to 1 part by weight of vegetable chitosan; And,
(C) adjusting the pH to be 4.5 to 5.5 by adding a basic substance;
Method for producing a female detergent composition comprising a. The method of claim 15,
Vegetable chitosan of the step (a), a method for producing a feminine cleanser composition, characterized in that prepared by heating, followed by separation, washing and drying of an aqueous alkali solution of mushrooms. The method of claim 15,
Method of manufacturing a female cleansing agent composition, characterized in that the first mixing stirring and the second mixing stirring of the step (b) is performed at room temperature. The method of claim 15,
The first mixing and stirring of the step (B) is made for 1 to 3 minutes, and the second mixing and stirring is for 20 minutes to 80 minutes.

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