CN108079165A - A kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt and preparation method thereof - Google Patents
A kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt and preparation method thereof Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
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Abstract
The present invention relates to medical product field, more particularly to a kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt and preparation method thereof.The purpose of the present invention is to solve the shortcomings of the prior art, a kind of compound female gynecological washing lotion is provided, specifically a kind of woman's washing lotion containing chitosan quaternary ammonium salt and preparation method thereof, it contains four kinds of chitosan oligosaccharide, chitosan quaternary ammonium salt, aloe and Cranberry principal components, with good Synergistic antimicrobial effect, its good antimicrobial effect and with certain overriding resistance effect, is appropriate for promoting and applying.
Description
Technical field
The present invention relates to medical product field, more particularly to a kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt and its
Preparation method.
Background technology
Female private part is the major organs of metabolic secretion, and introitus is two big system door of reproduction and uropoiesis, is easily felt
Contaminate a variety of diseases.Vaginitis, vulvitis etc. caused by bacterial infection, virus cause under pudendal pruritus, dense leukorrhea, are wet
Wart, cervical erosion etc. bring pain unable to describe in words to women, such as not usually prevention or in time treatment, not only influence woman
Woman is physically and mentally healthy, and can accelerate the aging of sexual organ.
Chinese patent CN102755260B in the prior art is mentioned and not only had using this cleaning health promoting liquid of fly maggot chitosan
There is antibiotic health effect, one layer of biomembrane can also be formed, slowly penetrated into histocyte, repair the histocyte of damage,
Inhibit duplication of viral DNA etc., sheerly in biological agent, and have no toxic side effect, will not more generate drug resistance phenomenon.The prior art
Middle Chinese patent CN103446177B is mentioned using two kinds of principal components of chitosan and Chlorhexidine, has good Synergistic antimicrobial effect
Fruit.
People have been selected using the conditioning liquid containing pure biologic formulation, and irritation reduces really, but most pure biology systems
Agent antibacterial action itself is weak, causes bacterium infection in the fabrication process, and bacterium severely exceeds, and makes more bacterial infestation private parts,
Harm health.In the majority there are many more conditioning liquid chlorine-containing component, chlorine is a kind of strong oxidizer, not only stimulates skin, injury private parts, and
And most beneficial bacteriums of private parts can be also killed, make flora disequilibrium, lower the resistance of the body, cause some pathogenic bacteria then
More breedings.
Effect of the various disinfectants of the prior art in terms for the treatment of and prevention is also preferable not to the utmost.It is therefore desirable to
Develop a kind of compound female gynecological washing lotion.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to a kind of compound female gynecological washing lotion is provided, specifically
A kind of woman's washing lotion containing chitosan quaternary ammonium salt and preparation method thereof, it contain chitosan oligosaccharide, chitosan quaternary ammonium salt, aloe and
Four kinds of principal components of Cranberry have good Synergistic antimicrobial effect, have good antimicrobial effect and overriding resistance effect.
The purpose of the present invention is achieved through the following technical solutions：
A kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt, the gynaecology of the chitosan quaternary ammonium salt are by following with washing lotion
The raw material of parts by weight is made：0.01-4 parts of chitosan oligosaccharide, 0.01-4 parts of chitosan quaternary ammonium salt, 0.5-1 parts of aloe extract, Cranberry
0.5-1 parts of extract, natural lactic acid 0.2-1 parts, 0.2-1 parts of DL- pyrrolidone sodium carboxylates solution (PCA-Na), surfactant
0.2-1 parts, 70-110 parts of purified water.
The preparation method of the aloe extract includes the following steps：
It chooses ripe Chinese aloe leaf and cleans peeling, aloe is beaten, adds 70% ethanol water of 4-8 times of volume of aloe slurry weight
Solution extracts, and Extracting temperature is 40-60 DEG C, and extraction time 1-3h filters after extraction, obtains first-time filtrate, to filter residue
It is middle add in 3-6 times of volume of its weight 70% ethanol water continue extract 1-3 it is small when, Extracting temperature be 40-60 DEG C, filter
To secondary filtrate, first-time filtrate and secondary filtrate are merged, amalgamation liquid is concentrated under reduced pressure, and is concentrated into every milliliter of water 1- containing crude drug
1.4g is dry to get aloe extract.
The preparation method of the Cranberry extract includes the following steps：
Cranberry fresh fruit is taken to crush, slurries and residue is collected, it is water-soluble to add in 80% ethyl alcohol that 10-14 times of its weight is measured
Liquid after being sufficiently mixed, is placed in ultrasonic extractor and is ultrasonically treated, extraction time 2-4h, 45-65 DEG C of Extracting temperature, after extraction
Filtering, obtains first-time filtrate, 70% ethanol water that 3-6 times of volume of its weight is added in into filter residue is placed in ultrasonic extractor
In continue to extract 2-3 it is small when, 45-65 DEG C of Extracting temperature, filtration obtains secondary filtrate, and first-time filtrate and secondary filtrate are merged,
Amalgamation liquid is concentrated under reduced pressure, and is concentrated into every milliliter of water 1-1.2g containing crude drug, dry to get Cranberry extract.
The chitosan oligosaccharide molecular weight is 1500~3000；The deacetylation of the chitosan quaternary ammonium salt is 90-98%；Shell gathers
The molecular weight ranges of sugared quaternary ammonium salt are 20,000~300,000；The surfactant is lecithin, in Tween 80, fatty glyceride
One or more.
Preferably, the gynaecology of the chitosan quaternary ammonium salt with washing lotion is made by the raw material of following weight parts：Chitosan oligosaccharide
0.08-2 parts, 0.08-2 parts of chitosan quaternary ammonium salt, 0.7-0.9 parts of aloe extract, 0.7-0.9 parts of Cranberry extract, naturally
0.4-0.8 parts of lactic acid, 0.4-0.8 parts of DL- pyrrolidone sodium carboxylates solution (PCA-Na), 0.4-0.8 parts of surfactant, purifying
80-100 parts of water.
Preferably, the gynaecology of the chitosan quaternary ammonium salt with washing lotion is made by the raw material of following weight parts：Chitosan oligosaccharide 0.1
Part, 0.1 part of chitosan quaternary ammonium salt, 0.8 part of aloe extract, 0.8 part of Cranberry extract, natural 0.6 part of lactic acid, DL- pyrroles
0.6 part of alkanone carboxylic acid sodium solution (PCA-Na), 0.6 part of surfactant, 90 parts of purified water.
The effect for each raw material that the present invention uses is as follows：
Chitosan is inhibited to the growth of various bacteria, shows the feature of similar antibiotic.Chitosan is to table
The experimental strains such as skin staphylococcus, staphylococcus aureus, Escherichia coli, pseudomonas aeruginosa, Candida albicans have
There are inhibitory action or even the Candida albicans for being difficult to inhibit to general antibiotic also to have inhibitory action.Macromolecular chitosan passes through
The interaction of the positive charge of the institute's band of itself and the negative electrical charge entrained by microbial cell film destroys the original knot of bacteria cell wall
Structure causes the leakage of cell component and plays antibacterial action.And the chitosan of small molecule, it is intracellular with the moon by penetrating into
The large biological molecule of ion occurs similar to " flocculation " effect, to upset the normal physiological function of cell, the biosynthesis of blocking dna,
So as to the breeding and growth that inhibit bacteria.As main disinfection component, it is proportionally added into mixture, it is therefore an objective to pass through it
Characteristic assists polyhexamethylene guanide, the residual bacterial do not killed completely is made to lose vitality, so as to reach enhancing sterilization effect.
The healing of the surface of a wound or wound is very important for patient, and wound mainly includes platelet aggregation, blood coagulates
Gu, fibrin formation, inflammatory reaction generation, matrix variation, cell Proliferation and regeneration, repair of damaged tissues and remodeling waited
Journey.Chitosan can promote the regeneration of epithelial cell, be proliferated to promote wound healing by mediated cell, pass through mild acute inflammation
Property reaction substantial amounts of polymorphonuclear cell and macrophage is attracted to remove fragment of tissue and blood clot.Chitosan can promote fiber finer
The migration of born of the same parents has stroma cell chemotactic, migration, activation, and accelerates cell Proliferation and tissue remodeling process, promotes skin
Tissue repair.The catabolite N-acetyl-glucosamine (NAG) of chitosan repairs the scar of tissue extremely important.Make as vagina
Externally used antimicrobial agent and surface of a wound care agent, although chitosan have in terms of the wound repair (such as in wound repair and nursing) compared with
Good effect, but in terms of antibiotic property, apparent deficiency, especially this form of washing lotion, are rinsed especially in terms of pathogenic bacteria are killed
Vagina when, active ingredient in washing lotion intravaginal retention time is short, effective concentration is low, a kind of ingredient of chitosan alone,
For in the serious and treatment of intractable gynecological disease, antibacterial action Shortcomings.
The water solubility of chitosan quaternary ammonium salt is got well than chitin and chitosan, can preferably play the work(of chitosan
Effect.Chitosan quaternary ammonium salt not only has the property of typical quaternary ammonium salt, such as bacteria resistance and moisture absorbability and moisture retentivity, and maintains
The performances such as the original good film forming of chitosan, flocculability, biocompatibility and biodegradation, preferably make use of chitosan
Biological activity；Chitosan quaternary ammonium salt has pathogenic microorganism very strong thermophilic film, adsorbs, merges with membranous structure, changes it
Permeability causes its content to leak and causes it dead；Have broad-spectrum antiseptic, antiviral activity, pathogenic microorganism is not easy to produce it
Raw drug resistance.
Chitosan oligosaccharide refers to that the molecular weight that 2~20 Glucosamines are formed by β (1-4) glucosides key connection is less than 3000 water
Soluble compound, it is to be obtained by chitin or chitosan through enzyme degradation or chemical degradation.Chitosan oligosaccharide is unique in natural sugar
A large amount of existing alkaline polysaccharides, also low with viscosity in addition to there is the property of similar chitosan, good water solubility, molecular weight
Small, the advantages that easily absorbing and is degradable, bioactivity and functional character are all more extensive compared with chitosan.Chitosan oligosaccharide has
It is antibacterial, inhibit tumour cell growth, strengthen liver function, prevent apoplexy, anti-peptic ulcer, reducing blood sugar and blood lipid, radioresistance,
There is the bioactivity of interferon generation and the biosynthesis for promoting protein and nucleic acid etc., chitosan oligosaccharide is known as 21 generation
It records new " human body environment-friendly agent ".
Cranberry, also known as mossberry, cranberry, English name Cranberry is Ericaceae Vaccinium crowberry subgenus
(scientific name：Oxycoccos also known as littlefruit craneberry subgenus) be commonly called as, the species of this subgenus are evergreen shrubs, are mainly grown in north half
In the nice and cool area acid peat soil earth of ball.Flower rediance, raceme.Red fruit can cook fruit and eat.At present in north
It is largely planted in some beautiful areas.Complex chemical composition contained by Cranberry plant has multiple biological activities.In recent years,
Domestic and foreign scholars have made more in-depth study to its pharmacological action, bioactivity and clinicing aspect from different perspectives respectively, are
Its clinical efficacy provides foundation, and research finds that Cranberry extract has inducing cell apoptosis, antiallergy and anti-inflammatory activity,
Especially a kind of natural antibacterial health fruit, be the various bacterium infections of the prevention daily urinary system of women, urethritis, cystitis,
The optimal natural food therapy of chronic nephropyelitis.
Aloe is Liliaceae perennial evergreen plant, and leafage is raw, big and plump, in seat shape or is born in stem top, leaf often drapes over one's shoulders pin
There is sharp tooth thorn at shape or the short width of leaf, edge.Inflorescence is umbrella shape, total shape, cone etc., and color is in red, yellow or tool redness of the skin or complexion spot, petal
Six, six pieces of gynoecium, the more joints of perianth base portion are into tubular.The chemical composition of aloe has aloe polysaccharide, barbaloin, aloe-emodin
Glycosides etc. has bactericidal effect, and anti-inflammatory effect moistens beautification function, the effect of letting out under stomach invigorating, and cardiac stimulant promoting blood circulation effect is immunized and regenerates
Effect, is immunized and antitumor action, detoxication, anti-aging effects ease pain, sedation, anti-sunlight function.
It is a further object of the present invention to provide a kind of preparation method of gynaecology's washing lotion of chitosan quaternary ammonium salt, including following
Step：
1) chitosan oligosaccharide of formula ratio is weighed, chitosan quaternary ammonium salt, aloe extract, Cranberry extract, natural lactic acid,
DL- pyrrolidone sodium carboxylates solution (PCA-Na), surfactant, purified water；
2) by the load weighted chitosan oligosaccharide of step 1), chitosan quaternary ammonium salt, aloe extract, Cranberry extract, DL- pyrroles
Alkanone carboxylic acid sodium solution (PCA-Na), surfactant are added in purified water, and stirring is to being completely dissolved；
3) add in natural lactic acid into the solution that step 2) prepares, adjust liquid pH value 4~6, stir evenly, after filtering
Sterilizing obtains gynaecology's washing lotion of chitosan.
The present invention is also claimed above-mentioned chitosan quaternary ammonium salt gynaecology and is preparing treatment vaginitis, cervicitis and outer with washing lotion
Purposes in cloudy scorching drug.
The present invention generates the mechanism of drug resistance according to bacterium, using composite antibacterial method, substantially increases its single use
The antibacterial range and sterilizing ability of medicine effectively prevent the chance that bacterium generates drug resistance, and more important is the present invention to select
With the chitosan quaternary ammonium salt with broad-spectrum antibacterial action and chitosan oligosaccharide pair and with anti-inflammatory, the aloe extract of bactericidal effect and climing
More certain kind of berries extract is bactericidal composition, belongs to non-antibiotic bactericidal composition, than selecting with microorganism, as the metabolism of actinomyces is produced
Object or the antibiotic of synthesis have more broad-spectrum antiseptic, are not easy to be bacterially decomposed, anti-drug resistance, and antibacterial effect is notable, nontoxic, nothing
It stimulates, good biocompatibility, bacterium can be isolated and kills bacterium, fungi, virus, skin and mucosa infection are prevented, to vagina etc.
The bacterium of position mucous membrane local infection generation, fungi, virus play isolation and protection.
The gynaecology of chitosan quaternary ammonium salt of the present invention has following technical advantage with washing lotion：
(1) present invention produces chitosan using chitosan oligosaccharide, chitosan quaternary ammonium salt, aloe extract and Cranberry extract
Gynaecology's washing lotion of quaternary ammonium salt, each raw material complement each other, and play the synergistic effect between raw material, reach the bacteriostasis property of the present invention
It is optimal, and with broad-spectrum antiseptic, anti-drug resistance.The present invention plays guarantor by using DL- pyrrolidone sodium carboxylate solution
Wet and moist effect, and there is no irritation to skin, it is safe.
(2) substance that the present invention uses will not destroy the original pH value of intravaginal and balance, and pathogenic bacteria will not be made to generate anti-medicine
Property, while the mucous layer of intravaginal can be nourished with depth, lubrication and the cleaning of vagina are kept, and there is irritation without other
The additives such as solvent, surfactant and moisturizer, avoid toxic side effect of such substance to skin.It is suitble to controlling for illness
It treats and routine health is nursed, it is nontoxic, green, environmentally friendly.
Specific embodiment
With reference to embodiment, the claim of the present invention is described in further detail, but do not formed pair
Any restrictions of the present invention.
Embodiment 1
A kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt, the gynaecology of the chitosan quaternary ammonium salt are by following with washing lotion
The raw material of parts by weight is made：0.01 part of chitosan oligosaccharide, 0.01 part of chitosan quaternary ammonium salt, 0.5 part of aloe extract, Cranberry extract
0.5 part, natural 0.2 part of lactic acid, 0.2 part of DL- pyrrolidone sodium carboxylates solution (PCA-Na), 0.2 part of surfactant, purified water
70 parts.
The preparation method of the aloe extract includes the following steps：
It chooses ripe Chinese aloe leaf and cleans peeling, aloe is beaten, 70% ethyl alcohol for adding 4 times of volumes of aloe slurry weight is water-soluble
Liquid extracts, and Extracting temperature is 40 DEG C, and extraction time 1h filters after extraction, obtains first-time filtrate, it is added in into filter residue
70% ethanol water of 3 times of volumes of weight continue extraction 1 it is small when, Extracting temperature be 40 DEG C, filtration obtain secondary filtrate, by one
Secondary filtrate and secondary filtrate merge, and amalgamation liquid is concentrated under reduced pressure, and are concentrated into every milliliter of water 1g containing crude drug, dry to be extracted to get aloe
Object.
The preparation method of the Cranberry extract includes the following steps：
Cranberry fresh fruit is taken to crush, collects slurries and residue, adds in 80% ethanol water of 10 times of amounts of its weight,
It after being sufficiently mixed, is placed in ultrasonic extractor and is ultrasonically treated, extraction time 2h, 45 DEG C of Extracting temperature is filtered after extraction, obtained
First-time filtrate, 70% ethanol water that 3 times of volumes of its weight are added in into filter residue are placed in ultrasonic extractor and continue extraction 2
Hour, 45 DEG C of Extracting temperature, filtration obtains secondary filtrate, first-time filtrate and secondary filtrate is merged, amalgamation liquid is concentrated under reduced pressure, dense
Every milliliter of water 1g containing crude drug is reduced to, it is dry to get Cranberry extract.
The chitosan oligosaccharide molecular weight is 1500；The deacetylation of the chitosan quaternary ammonium salt is 90%；Chitosan quaternary ammonium salt
Molecular weight ranges 20,000；The surfactant is lecithin.
A kind of preparation method of gynaecology's washing lotion of chitosan quaternary ammonium salt, comprises the following steps：
1) chitosan oligosaccharide of formula ratio is weighed, chitosan quaternary ammonium salt, aloe extract, Cranberry extract, natural lactic acid,
DL- pyrrolidone sodium carboxylates solution (PCA-Na), surfactant, purified water.
2) by the load weighted chitosan oligosaccharide of step 1), chitosan quaternary ammonium salt, aloe extract, Cranberry extract, DL- pyrroles
Alkanone carboxylic acid sodium solution (PCA-Na), surfactant are added in purified water, and stirring is to being completely dissolved.
3) add in natural lactic acid into the solution that step 2) prepares, adjust liquid pH value 4, stir evenly, go out after filtering
Bacterium obtains gynaecology's washing lotion of chitosan.
Embodiment 2
A kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt, the gynaecology of the chitosan quaternary ammonium salt are by following with washing lotion
The raw material of parts by weight is made：4 parts of chitosan oligosaccharide, 4 parts of chitosan quaternary ammonium salt, 1 part of aloe extract, 1 part of Cranberry extract, naturally
1 part of lactic acid, 1 part of DL- pyrrolidone sodium carboxylates solution (PCA-Na), 1 part of surfactant, 110 parts of purified water.
The preparation method of the aloe extract includes the following steps：
It chooses ripe Chinese aloe leaf and cleans peeling, aloe is beaten, 70% ethyl alcohol for adding 8 times of volumes of aloe slurry weight is water-soluble
Liquid extracts, and Extracting temperature is 60 DEG C, and extraction time 3h filters after extraction, obtains first-time filtrate, it is added in into filter residue
70% ethanol water of 6 times of volumes of weight continue extraction 3 it is small when, Extracting temperature be 60 DEG C, filtration obtain secondary filtrate, by one
Secondary filtrate and secondary filtrate merge, and amalgamation liquid is concentrated under reduced pressure, and are concentrated into every milliliter of water 1.4g containing crude drug, and drying carries to get aloe
Take object.
The preparation method of the Cranberry extract includes the following steps：
Cranberry fresh fruit is taken to crush, collects slurries and residue, adds in 80% ethanol water of 14 times of amounts of its weight,
It after being sufficiently mixed, is placed in ultrasonic extractor and is ultrasonically treated, extraction time 4h, 65 DEG C of Extracting temperature is filtered after extraction, obtained
First-time filtrate, 70% ethanol water that 6 times of volumes of its weight are added in into filter residue are placed in ultrasonic extractor and continue extraction 3
Hour, 65 DEG C of Extracting temperature, filtration obtains secondary filtrate, first-time filtrate and secondary filtrate is merged, amalgamation liquid is concentrated under reduced pressure, dense
Every milliliter of water 1.2g containing crude drug is reduced to, it is dry to get Cranberry extract.
The chitosan oligosaccharide molecular weight is 3000；The deacetylation of the chitosan quaternary ammonium salt is 98%；Chitosan quaternary ammonium salt
Molecular weight ranges 300,000；The surfactant is Tween 80.
A kind of preparation method of gynaecology's washing lotion of chitosan quaternary ammonium salt, comprises the following steps：
1) chitosan oligosaccharide of formula ratio is weighed, chitosan quaternary ammonium salt, aloe extract, Cranberry extract, natural lactic acid,
DL- pyrrolidone sodium carboxylates solution (PCA-Na), surfactant, purified water.
2) by the load weighted chitosan oligosaccharide of step 1), chitosan quaternary ammonium salt, aloe extract, Cranberry extract, DL- pyrroles
Alkanone carboxylic acid sodium solution (PCA-Na), surfactant are added in purified water, and stirring is to being completely dissolved.
3) add in natural lactic acid into the solution that step 2) prepares, adjust liquid pH value 6, stir evenly, go out after filtering
Bacterium obtains gynaecology's washing lotion of chitosan.
Embodiment 3
A kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt, the gynaecology of the chitosan quaternary ammonium salt are by following with washing lotion
The raw material of parts by weight is made：0.08-2 parts of chitosan oligosaccharide, 0.08 part of chitosan quaternary ammonium salt, 0.7 part of aloe extract, Cranberry extraction
0.7 part of object, natural 0.4 part of lactic acid, 0.4 part of DL- pyrrolidone sodium carboxylates solution, 0.4 part of surfactant, 80 parts of purified water.
The preparation method of the aloe extract includes the following steps：
It chooses ripe Chinese aloe leaf and cleans peeling, aloe is beaten, 70% ethyl alcohol for adding 5 times of volumes of aloe slurry weight is water-soluble
Liquid extracts, and Extracting temperature is 45 DEG C, and extraction time 1.5h filters after extraction, obtains first-time filtrate, added in into filter residue
70% ethanol water of 4 times of volumes of its weight continue extraction 1.5 it is small when, Extracting temperature be 45 DEG C, filtration obtain secondary filtrate,
First-time filtrate and secondary filtrate are merged, amalgamation liquid is concentrated under reduced pressure, and is concentrated into every milliliter of water 1.1g containing crude drug, dry to get reed
Luxuriant growth extract.
The preparation method of the Cranberry extract includes the following steps：
Cranberry fresh fruit is taken to crush, collects slurries and residue, adds in 80% ethanol water of 11 times of amounts of its weight,
It after being sufficiently mixed, is placed in ultrasonic extractor and is ultrasonically treated, extraction time 2.5h, 50 DEG C of Extracting temperature is filtered after extraction, obtained
To first-time filtrate, 70% ethanol water that 4 times of volumes of its weight are added in into filter residue is placed in ultrasonic extractor and continues to carry
Take 2.5 it is small when, 50 DEG C of Extracting temperature, filtration obtains secondary filtrate, and first-time filtrate and secondary filtrate are merged, and amalgamation liquid decompression is dense
Contracting is concentrated into every milliliter of water 1.05g containing crude drug, dry to get Cranberry extract.
The chitosan oligosaccharide molecular weight is 2000；The deacetylation of the chitosan quaternary ammonium salt is 92%；Chitosan quaternary ammonium salt
Molecular weight be 100,000；The surfactant is neopelex.
A kind of preparation method of gynaecology's washing lotion of chitosan quaternary ammonium salt, comprises the following steps：
1) chitosan oligosaccharide of formula ratio is weighed, chitosan quaternary ammonium salt, aloe extract, Cranberry extract, natural lactic acid,
DL- pyrrolidone sodium carboxylates solution (PCA-Na), surfactant, purified water.
2) by the load weighted chitosan oligosaccharide of step 1), chitosan quaternary ammonium salt, aloe extract, Cranberry extract, DL- pyrroles
Alkanone carboxylic acid sodium solution (PCA-Na), surfactant are added in purified water, and stirring is to being completely dissolved.
3) add in natural lactic acid into the solution that step 2) prepares, adjust liquid pH value 4.5, stir evenly, after filtering
Sterilizing obtains gynaecology's washing lotion of chitosan.
Embodiment 4
A kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt, the gynaecology of the chitosan quaternary ammonium salt are by following with washing lotion
The raw material of parts by weight is made：2 parts of chitosan oligosaccharide, 2 parts of chitosan quaternary ammonium salt, 0.9 part of aloe extract, 0.9 part of Cranberry extract,
Natural 0.8 part of lactic acid, 0.8 part of DL- pyrrolidone sodium carboxylates solution (PCA-Na), 0.8 part of surfactant, 100 parts of purified water.
The preparation method of the aloe extract includes the following steps：
It chooses ripe Chinese aloe leaf and cleans peeling, aloe is beaten, 70% ethyl alcohol for adding 7 times of volumes of aloe slurry weight is water-soluble
Liquid extracts, and Extracting temperature is 55 DEG C, and extraction time 2.5h filters after extraction, obtains first-time filtrate, added in into filter residue
70% ethanol water of 5.5 times of volumes of its weight continue extraction 2.5 it is small when, Extracting temperature be 55 DEG C, filtration obtain secondary filter
Liquid merges first-time filtrate and secondary filtrate, and amalgamation liquid is concentrated under reduced pressure, and is concentrated into every milliliter of water 1.3g containing crude drug, dry to get
Aloe extract.
The preparation method of the Cranberry extract includes the following steps：
Cranberry fresh fruit is taken to crush, collects slurries and residue, adds in 80% ethanol water of 13 times of amounts of its weight,
It after being sufficiently mixed, is placed in ultrasonic extractor and is ultrasonically treated, extraction time 3.5h, 60 DEG C of Extracting temperature is filtered after extraction, obtained
To first-time filtrate, 70% ethanol water that 3-6 times of volume of its weight is added in into filter residue is placed in ultrasonic extractor and continues
Extract 2.5 it is small when, 60 DEG C of Extracting temperature, filtration obtains secondary filtrate, and first-time filtrate and secondary filtrate are merged, amalgamation liquid decompression
Concentration is concentrated into every milliliter of water 1.1g containing crude drug, dry to get Cranberry extract.
The chitosan oligosaccharide molecular weight is 2500；The deacetylation of the chitosan quaternary ammonium salt is 96%；Chitosan quaternary ammonium salt
Molecular weight ranges 200,000；The surfactant is fatty glyceride.
A kind of preparation method of gynaecology's washing lotion of chitosan quaternary ammonium salt, comprises the following steps：
1) chitosan oligosaccharide of formula ratio is weighed, chitosan quaternary ammonium salt, aloe extract, Cranberry extract, natural lactic acid,
DL- pyrrolidone sodium carboxylates solution (PCA-Na), surfactant, purified water.
2) by the load weighted chitosan oligosaccharide of step 1), chitosan quaternary ammonium salt, aloe extract, Cranberry extract, DL- pyrroles
Alkanone carboxylic acid sodium solution (PCA-Na), surfactant are added in purified water, and stirring is to being completely dissolved.
3) add in natural lactic acid into the solution that step 2) prepares, adjust liquid pH value 5.5, stir evenly, after filtering
Sterilizing obtains gynaecology's washing lotion of chitosan.
Embodiment 5
A kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt, the gynaecology of the chitosan quaternary ammonium salt are by following with washing lotion
The raw material of parts by weight is made：0.1 part of chitosan oligosaccharide, 0.1 part of chitosan quaternary ammonium salt, 0.8 part of aloe extract, Cranberry extract
0.8 part, natural 0.6 part of lactic acid, 0.6 part of DL- pyrrolidone sodium carboxylates solution (PCA-Na), 0.6 part of surfactant, purified water
90 parts.
The preparation method of the aloe extract includes the following steps：
It chooses ripe Chinese aloe leaf and cleans peeling, aloe is beaten, 70% ethyl alcohol for adding 6 times of volumes of aloe slurry weight is water-soluble
Liquid extracts, and Extracting temperature is 50 DEG C, and extraction time 2h filters after extraction, obtains first-time filtrate, it is added in into filter residue
70% ethanol water of 4 times of volumes of weight continue extract 1-3 it is small when, Extracting temperature be 50 DEG C, filtration obtain secondary filtrate, will
First-time filtrate and secondary filtrate merge, and amalgamation liquid is concentrated under reduced pressure, and are concentrated into every milliliter of water 1.2g containing crude drug, dry to get aloe
Extract.
The preparation method of the Cranberry extract includes the following steps：
Cranberry fresh fruit is taken to crush, collects slurries and residue, adds in 80% ethanol water of 12 times of amounts of its weight,
It after being sufficiently mixed, is placed in ultrasonic extractor and is ultrasonically treated, extraction time 3h, 55 DEG C of Extracting temperature is filtered after extraction, obtained
First-time filtrate, 70% ethanol water that 4 times of volumes of its weight are added in into filter residue are placed in ultrasonic extractor and continue to extract
2.5 it is small when, 55 DEG C of Extracting temperature, filtration obtains secondary filtrate, and first-time filtrate and secondary filtrate are merged, and amalgamation liquid decompression is dense
Contracting is concentrated into every milliliter of water 1.1g containing crude drug, dry to get Cranberry extract.
The chitosan oligosaccharide molecular weight is 2000；The deacetylation of the chitosan quaternary ammonium salt is 95%；Chitosan quaternary ammonium salt
Molecular weight ranges 150,000；The surfactant is Tween 80.
A kind of preparation method of gynaecology's washing lotion of chitosan quaternary ammonium salt, comprises the following steps：
1) chitosan oligosaccharide of formula ratio is weighed, chitosan quaternary ammonium salt, aloe extract, Cranberry extract, natural lactic acid,
DL- pyrrolidone sodium carboxylates solution (PCA-Na), surfactant, purified water.
2) by the load weighted chitosan oligosaccharide of step 1), chitosan quaternary ammonium salt, aloe extract, Cranberry extract, DL- pyrroles
Alkanone carboxylic acid sodium solution (PCA-Na), surfactant are added in purified water, and stirring is to being completely dissolved.
3) add in natural lactic acid into the solution that step 2) prepares, adjust liquid pH value 5, stir evenly, go out after filtering
Bacterium obtains gynaecology's washing lotion of chitosan.
Therapeutic effect of the washing lotion to gynaecological imflammation of 6 gynaecology of the present invention of embodiment
Inventor has carried out system clinical observation to 180 intractable gynaecological imflammation volunteers, and 20~50 years old age is wherein
Female vulva inflammation, vaginitis, each 60 of cervicitis are selected, respectively using gynaecology's washing lotion of embodiment 1,2,3,4,5, each group will
Hope person is vagina administration, and once, continuous use after a week, counts the therapeutic effect after medication for each medication sooner or later.Its result is such as
Shown in following table.
Therapeutic effect of the washing lotion to gynaecological imflammation of 1 gynaecology of the present invention of table
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| Vulvitis symptom mitigates | 58 | 57 | 58 | 58 | 60 |
| Vaginitis symptom mitigates | 56 | 56 | 57 | 58 | 59 |
| Cervicitis symptom mitigates | 54 | 55 | 56 | 56 | 58 |
Above-mentioned clinical statistics data shows to be directly administered as vagina, and the present invention mitigates patient symptom effect to symptom management
Fruit is apparent.Gynaecology of the present invention is extensive for being applicable in disease when treating gynaecological imflammation with washing lotion, and treated effect is high, energy
Enough preferable compliances and quality of life for improving patient.It is suitble to the treatment of illness and routine health nursing, it is nontoxic, green
Color, environmental protection.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope, every modification made using description of the invention and appended claims or equivalent substitution are protected, is wrapped
It includes in the scope of patent protection of the present invention.
Claims (9)
1. a kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt, which is characterized in that it is made by the raw material of following weight parts：
0.01-4 parts of chitosan oligosaccharide, 0.01-4 parts of chitosan quaternary ammonium salt, 0.5-1 parts of aloe extract, 0.5-1 parts of Cranberry extract, day
Right 0.2-1 parts of lactic acid, 0.2-1 parts of DL- pyrrolidone sodium carboxylate solution, 0.2-1 parts of surfactant, 70-110 parts of purified water.
A kind of 2. gynaecology's washing lotion containing chitosan quaternary ammonium salt according to claim 1, which is characterized in that the aloe
The preparation method of extract includes the following steps：
It chooses ripe Chinese aloe leaf and cleans peeling, aloe is beaten, adds 70% ethanol water of 4-8 times of volume of aloe slurry weight
It extracts, Extracting temperature is 40-60 DEG C, and extraction time 1-3h filters after extraction, obtains first-time filtrate, is added into filter residue
Enter 3-6 times of volume of its weight 70% ethanol water continue extract 1-3 it is small when, Extracting temperature be 40-60 DEG C, filtration obtain two
Secondary filtrate merges first-time filtrate and secondary filtrate, and amalgamation liquid is concentrated under reduced pressure, and is concentrated into every milliliter of water 1-1.4g containing crude drug, does
It is dry to get aloe extract.
3. a kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt according to claim 1, which is characterized in that it is described it is climing more
The preparation method of certain kind of berries extract includes the following steps：
Cranberry fresh fruit is taken to crush, slurries and residue is collected, adds in 80% ethanol water that 10-14 times of its weight is measured,
After being sufficiently mixed, it is placed in ultrasonic extractor and is ultrasonically treated, extraction time 2-4h, 45-65 DEG C of Extracting temperature, mistake after extraction
Filter, obtains first-time filtrate, 70% ethanol water that 3-6 times of volume of its weight is added in into filter residue is placed in ultrasonic extractor
Continue to extract 2-3 it is small when, 45-65 DEG C of Extracting temperature, filtration obtains secondary filtrate, and first-time filtrate and secondary filtrate are merged, and closes
And liquid is concentrated under reduced pressure, and is concentrated into every milliliter of water 1-1.2g containing crude drug, it is dry to get Cranberry extract.
4. a kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt according to claim 1, which is characterized in that the shell is few
Glycan molecule amount is 1500~3000；The deacetylation of the chitosan quaternary ammonium salt is 90-98%；The molecular weight of chitosan quaternary ammonium salt
Scope is 20,000~300,000.
A kind of 5. gynaecology's washing lotion containing chitosan quaternary ammonium salt according to claim 1, which is characterized in that the surface
Activating agent is lecithin, the one or more in Tween 80, fatty glyceride.
6. a kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt according to claim 1, which is characterized in that it is under
The raw material for stating parts by weight is made：0.08-2 parts of chitosan oligosaccharide, 0.08-2 parts of chitosan quaternary ammonium salt, 0.7-0.9 parts of aloe extract are climing
More certain kind of berries extract 0.7-0.9 parts, natural lactic acid 0.4-0.8 parts, 0.4-0.8 parts of DL- pyrrolidone sodium carboxylate solution, surface-active
0.4-0.8 parts of agent, 80-100 parts of purified water.
7. a kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt according to claim 1, which is characterized in that it is under
The raw material for stating parts by weight is made：0.1 part of chitosan oligosaccharide, 0.1 part of chitosan quaternary ammonium salt, 0.8 part of aloe extract, Cranberry extract
0.8 part, natural 0.6 part of lactic acid, 0.6 part of DL- pyrrolidone sodium carboxylates solution, 0.6 part of surfactant, 90 parts of purified water.
8. a kind of preparation method according to claim 1-3 any one of them gynaecology washing lotion, it is characterized in that including following step
Suddenly：
1) chitosan oligosaccharide of formula ratio, chitosan quaternary ammonium salt, aloe extract, Cranberry extract, natural lactic acid, DL- pyrroles are weighed
Pyrrolidone carboxylic acid's sodium solution, surfactant, purified water；
2) by the load weighted chitosan oligosaccharide of step 1), chitosan quaternary ammonium salt, aloe extract, Cranberry extract, DL- pyrrolidones
Carboxylic acid sodium solution, surfactant are added in purified water, and stirring is to being completely dissolved；
3) add in natural lactic acid into the solution that step 2) prepares, adjust liquid pH value 4~6, stir evenly, sterilize after filtering
Obtain gynaecology's washing lotion of chitosan.
9. treatment vulvitis, vaginitis and cervicitis are being prepared according to claim 1-7 any one of them gynaecology washing lotion
Purposes in drug.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109568650A (en) * | 2018-12-19 | 2019-04-05 | 广州润虹医药科技股份有限公司 | A kind of chitosan quaternary ammonium salt antiseptic dressing and preparation method thereof |
| CN110302302A (en) * | 2019-08-06 | 2019-10-08 | 阿谷巴科技（唐山）有限公司 | A kind of antibacterial liquid formulations |
| WO2020054937A1 (en) * | 2018-09-14 | 2020-03-19 | 주식회사 엔도비전 | Feminine cleanser composition using vegetable chitosan, preparation method therefor, and feminine cleanser comprising same |
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| CN101829041A (en) * | 2010-04-30 | 2010-09-15 | 北京欧凯纳斯科技有限公司 | Sterilizing and itch-relieving gynaecological lotion |
| CN104998160A (en) * | 2015-07-17 | 2015-10-28 | 郭芝学 | Gynecological lotion |
| CN106727319A (en) * | 2017-01-09 | 2017-05-31 | 广州润虹医药科技有限公司 | A kind of gynaecology's foaming agent and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101829041A (en) * | 2010-04-30 | 2010-09-15 | 北京欧凯纳斯科技有限公司 | Sterilizing and itch-relieving gynaecological lotion |
| CN104998160A (en) * | 2015-07-17 | 2015-10-28 | 郭芝学 | Gynecological lotion |
| CN106727319A (en) * | 2017-01-09 | 2017-05-31 | 广州润虹医药科技有限公司 | A kind of gynaecology's foaming agent and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020054937A1 (en) * | 2018-09-14 | 2020-03-19 | 주식회사 엔도비전 | Feminine cleanser composition using vegetable chitosan, preparation method therefor, and feminine cleanser comprising same |
| CN109568650A (en) * | 2018-12-19 | 2019-04-05 | 广州润虹医药科技股份有限公司 | A kind of chitosan quaternary ammonium salt antiseptic dressing and preparation method thereof |
| CN110302302A (en) * | 2019-08-06 | 2019-10-08 | 阿谷巴科技（唐山）有限公司 | A kind of antibacterial liquid formulations |
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