CN109602813A - A kind of bacteriostatic gel and preparation method thereof - Google Patents

A kind of bacteriostatic gel and preparation method thereof Download PDF

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Publication number
CN109602813A
CN109602813A CN201910130070.1A CN201910130070A CN109602813A CN 109602813 A CN109602813 A CN 109602813A CN 201910130070 A CN201910130070 A CN 201910130070A CN 109602813 A CN109602813 A CN 109602813A
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parts
extract
cnidium
oil
composition
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林敏�
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Jiangxi Dengyun Health Beauty Industry Internet Ltd
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Jiangxi Dengyun Health Beauty Industry Internet Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/285—Aucklandia
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    • A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
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    • A61K36/18—Magnoliophyta (angiosperms)
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    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
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Abstract

The invention discloses a kind of bacteriostatic gels, it include: 0.05-0.3 parts of kuh-seng root extract, 0.05-0.3 parts of cnidium monnieri berry extract, 0.1-0.5 parts of houttuynia extract, 0.05-0.3 parts of Herba Saussureae Involueratae extract, 0.3-0.8 parts of Fructus Kochiae extract, 0.05-0.3 parts of peppermint oil, 0.05-0.3 parts of cnidium oil, 0.05-0.3 parts of rilanit special；0.4-0.6 parts of ethyl alcohol, 0.1-0.2 parts of chlorhexidine acetate；1-3 parts of hydroxyethyl cellulose, 4-6 parts of glycerol, 1-3 parts of propylene glycol, 88-90 parts of water.The present invention is by the way that by the kuh-seng root extract of certain content, cnidium monnieri berry extract, houttuynia extract, Herba Saussureae Involueratae extract, Fructus Kochiae extract, totally five kinds of Chinese medical extracts carry out each components 5 and match, cooperate peppermint oil, cnidium oil, rilanit special, and the synergistic function of drug effect, it is aided with the components such as the chlorhexidine acetate of certain content, the bacteriostatic gel being prepared to Escherichia coli, staphylococcus aureus, Candida albicans bacteriostasis rate have 90% or more fungistatic effect, female vulva itch, peculiar smell, the symptom of vaginitis can significantly be mitigated.

Description

A kind of bacteriostatic gel and preparation method thereof
Technical field
The present invention relates to pharmaceutical technology fields, and in particular to a kind of bacteriostatic gel and preparation method thereof.
Background technique
Women's health is more and more paid close attention to, and various Gynecological health articles occurs.General health treatment is more For oral preparation, its health effect is very unobvious, has larger side effect, and hormone-content is high, easily leads to human body interior point Secrete imbalance.External drug is big to skin irritation, allergic symptom easily occurs and the problem of without bactericidal effect, and in the market mostly For number product based on Chinese materia medica preparation, general fungistatic effect is bad.Furthermore there are also suppository classes, lotion for the antimicrobial for the treatment of gynaecological imflammation Class, spray class etc., but most drugs can generate destruction to genital tract environment, mucous membrane itself secreting function, so as to cause reproduction The decline of local immunity, and pathogenic bacteria is caused to develop drug resistance and drug resistance, final inflammation recurrent exerbation.
It, should if Chinese invention patent application CN201210039685.1 describes a kind of germicide for skin and preparation method thereof Germicide for skin includes ginkgo leaf juice, feverfew juice, RADIX SOLANI juice, kudzuvine root juice, argy wormwood leaf juice, purslane extract, Bulbus Allii Fistulosi juice, distillation The components such as water.But the antibacterial agent fungistatic effect is poor, and cannot effectively treat gynecological disease and cleaning skin, Chinese materia medica preparation conduct Bacteriostatic agent, fungistatic effect are poor.
Summary of the invention
It is in view of the above-mentioned drawbacks of the prior art or insufficient, the primary purpose of the present invention is that providing a kind of fungistatic effect Good, curative effect is stablized, and is able to maintain the clean bacteriostatic gel of women privates.The gelling agent is to Escherichia coli, Staphylococcus aureus Bacterium, Candida albicans bacteriostasis rate have 90% or more fungistatic effect, can significantly mitigate female vulva itch, peculiar smell, vagina Scorching symptom.
Another object of the present invention is to provide the preparation method of above-mentioned bacteriostatic gel.
Bacteriostatic gel of the present invention, by weight, including following component:
0.05-0.3 parts of kuh-seng root extract, 0.05-0.3 parts of cnidium monnieri berry extract, 0.1-0.5 parts of houttuynia extract, saussurea involucrata 0.05-0.3 parts of flower extract, 0.3-0.8 parts of Fructus Kochiae extract, 0.05-0.3 parts of peppermint oil, 0.05-0.3 parts of cnidium oil, hydrogen Change castor oil 0.05-0.3 parts；
0.4-0.6 parts of ethyl alcohol, 0.1-0.2 parts of chlorhexidine acetate；
1-3 parts of hydroxyethyl cellulose, 4-6 parts of glycerol, 1-3 parts of propylene glycol, 88-90 parts of water.
Preferably, bacteriostatic gel of the present invention, by weight, including following component:
0.1 part of kuh-seng root extract, 0.1 part of cnidium monnieri berry extract, 0.2 part of houttuynia extract, 0.1 part of Herba Saussureae Involueratae extract, 0.5 part of Fructus Kochiae extract, 0.1 part of peppermint oil, 0.1 part of cnidium oil, 0.1 part of rilanit special；
0.5 part of ethyl alcohol, 0.12 part of chlorhexidine acetate；
2 parts of hydroxyethyl cellulose, 5 parts of glycerol, 2 parts of propylene glycol, 89.08 parts of water.
Radix sophorae is the root of kuh-seng Sophora flavescens Ait., and bitter is cold in nature, has heat-clearing and damp-drying drug, diuresis Antipyretic, wind-dispelling stops skin and itches, bitter taste stomach invigorating, and the function of expelling parasite sterilization cures mainly enteritis and dysentery, enterorrhagia, hemorrhoid hemorrhage, hepatitis jaundice, Eczema, pudendal pruritus, psoriasis, boil, lambliasis.
Cnidium monnieri fruit is the dry mature fruit of umbelliferae cnidium monnieri Cnidium monnieri (L.) Cuss..It is distributed in The ground such as East China, Central-South, southwestern, northwest, North China, northeast.With the effect of eliminating dampness wind-dispelling, desinsection is antipruritic, warming kidney and enhancing body.It is usually used in Pruritus vulvae leukorrhagia, eczema itch, arthritis with fixed pain caused by dampness pain in the loins, impotence due to deficiency of the kidney, uterus cold infertile.
Cordate houttuynia is the drying above water of Saururaceae herbaceos perennial heartleaf houttuynia, acrid flavour, cold.It can heat-clearing solution Sore, promoting urination and removing dampness, stomach strengthening and digestion promoting are treated in poison, row's pain detumescence.With control real heat, heat toxin, damp evil, disease heat be suffer from pulmonary abscess, sore swollen toxin, Hemorrhoidal hemorrhage, retention of heat in the spleen and stomach etc..
Lanatechead saussurea herb with flower is perennial herb, 15-35 centimetres high.Warm-natured, sweet-bitter flavor enters the warp of liver,spleen,kidney three, and tool is except cold, strong Sun, menstruation regulating, hemostasis function, for treating impotence, waist and knee weakness, menoxenia, metrorrhagia and metrostaxis leukorrhagia, rheumatic arthritis and outer Hurt the diseases such as bleeding.
The fruit of summer cypress is the dry mature fruit of chenopod summer cypress, and China's most area is distributed, cold in nature, sweet in flavor Hardship has clearing heat and promoting diuresis, dispelling wind and arresting itching and other effects, for diseases such as difficulty and pain in micturition, pruritus vulvae leukorrhagia, rubeola, eczema, pruituses. It mainly contains the chemical components such as triterpenoid saponin, volatile oil and flavones, has resisting pathogenic microbes, anti-inflammatory, antiallergy, hypoglycemic With the pharmacological activity such as anti-mucosal lesion, modern studies have found that fruit of summer cypress water extract has centainly a variety of common pathogens of gynaecological disease Inhibiting effect, gynaecological disease control aspect have a better effect.
Bacteriostatic gel of the present invention mainly has heat-clearing by radix sophorae, cnidium monnieri fruit, cordate houttuynia, lanatechead saussurea herb with flower, five kinds of the fruit of summer cypress Eliminating dampness, desinsection is antipruritic, the Chinese medical extract composition of wind-dispelling removing toxic substances, auxiliary to match peppermint oil, cnidium oil, rilanit special, plays Chinese medicine The integrally-regulated advantage of compound, can significantly inhibit the growth and breeding of various bacteria, to Escherichia coli, staphylococcus aureus, The bacteriostasis rate of Candida albicans etc. has 90% or more fungistatic effect.
In addition, the present invention also provides the preparation methods of above-mentioned bacteriostatic gel, comprising the following steps:
(1) by hydroxyethyl cellulose, glycerol, mixed with propylene glycol, water is added after hydroxyethyl cellulose dissolution in stirring, and stirring adds Heat keeps the temperature 20 minutes to 95 DEG C, and composition A is made；
By kuh-seng root extract, cnidium monnieri berry extract, houttuynia extract, Herba Saussureae Involueratae extract, Fructus Kochiae extract, peppermint Oil, cnidium oil, rilanit special mixing, are made composition B；
Chlorhexidine acetate is dissolved in ethyl alcohol, composition C is made；
(2) composition A is cooled to 45 DEG C, composition B is then added, stirs evenly；
(3) continue to be cooled to 40 DEG C or less obtained gels under stiring, composition C is added, stirs evenly, obtains bacteriostatic gel Agent.
The bacteriostatic gel that the present invention is prepared, when use can it is filling in disposable vaginal to being administered in gel device, 3.5g is used every time.
Compared with prior art, beneficial effects of the present invention are as follows:
1) present invention is by extracting the kuh-seng root extract of certain content, cnidium monnieri berry extract, houttuynia extract, lanatechead saussurea herb with flower Totally five kinds of Chinese medical extracts progress each components 5 match, cooperate peppermint oil, cnidium oil, rilanit special for object, Fructus Kochiae extract, with And the synergistic function of drug effect, it is aided with the components such as the chlorhexidine acetate of certain content, the bacteriostatic gel being prepared is to big Enterobacteria, staphylococcus aureus, Candida albicans bacteriostasis rate have 90% or more fungistatic effect, can significantly mitigate female Property pruritus vulvue, peculiar smell, the symptom of vaginitis, and uneasy to recur, so as to effectively keep women privates to clean.
2) preparation method of bacteriostatic gel of the present invention, it is simple to operation, it is suitable for large-scale industrial production.
Specific embodiment
Further illustrate that the present invention, following embodiment are the specific embodiment party of the present invention below by specific embodiment Formula, but embodiments of the present invention are not limited by following embodiments.
Each component employed in the embodiment of the present invention is commercially available in market.
The inhibitory effect measuring method of the gelling agent, specific as follows:
(1) strain inhibitory effect, which measures strain used, see the table below 1:
Table 1
Experimental strain Assay medium Cultivation temperature/DEG C Incubation time
Escherichia coli Pancreas junket soya peptone agar medium or pancreas junket soya peptone fluid nutrient medium 30-35 18-24h
Staphylococcus aureus Pancreas junket soya peptone agar medium or pancreas junket soya peptone fluid nutrient medium 30-35 18-24h
Candida albicans Pancreas junket soya peptone agar medium or pancreas junket soya peptone fluid nutrient medium 20-25 24-48h
(2) prepared by bacterium solution
If agar culture, suitable 0.9% aseptic sodium chloride solution is added and elutes the culture of agar surface, and by bacterium Suspension moves in sterile test tube, and being diluted with 0.9% aseptic sodium chloride solution and every 1ml is made containing bacterium number is about 108The bacterium of cfu is outstanding Liquid；If fluid nutrient medium, thalline were collected by centrifugation, is diluted with 0.9% aseptic sodium chloride solution and every 1ml is made containing bacterium number and be about 108The bacteria suspension of cfu.
If being placed at room temperature after bacterium solution preparation, used in Ying 2h；If being stored in 2-8 DEG C, can be used interior for 24 hours.
(3) it is inoculated with
To each strain, 3-5 parts of inventive gel are taken, is directly inoculated with；Or appropriate inventive gel is taken to be transferred to 9-15 respectively In a suitable sterile chamber (every 3-5 container is inoculated with a strain), connecing bacterium amount in 1g or 1ml gel is 105-106Cfu connects The volume of kind bacterium solution must not exceed the 1% of inoculation gel volume；It is sufficiently mixed, is uniformly distributed the strain in gel, then sets 20-25 DEG C of stored protected from light.
(4) survival Counting alive microbial
In defined interval time, 1ml(g is sampled from above-mentioned each container respectively), bacterium number contained in every part of sample is tested, To be converted into corresponding bacteriostasis rate.
Embodiment 1
A kind of bacteriostatic gel, by weight, including following component:
0.1 part of kuh-seng root extract, 0.1 part of cnidium monnieri berry extract, 0.2 part of houttuynia extract, 0.1 part of Herba Saussureae Involueratae extract, 0.5 part of Fructus Kochiae extract, 0.1 part of peppermint oil, 0.1 part of cnidium oil, 0.1 part of rilanit special；
0.5 part of ethyl alcohol, 0.12 part of chlorhexidine acetate；
2 parts of hydroxyethyl cellulose, 5 parts of glycerol, 2 parts of propylene glycol, 89.08 parts of water.
The preparation method of bacteriostatic gel of the present invention, includes the following steps:
(1) by hydroxyethyl cellulose, glycerol, mixed with propylene glycol, water is added after hydroxyethyl cellulose dissolution in stirring, and stirring adds Heat keeps the temperature 20 minutes to 95 DEG C, and composition A is made；
By kuh-seng root extract, cnidium monnieri berry extract, houttuynia extract, Herba Saussureae Involueratae extract, Fructus Kochiae extract, peppermint Oil, cnidium oil, rilanit special mixing, are made composition B；
Chlorhexidine acetate is dissolved in ethyl alcohol, composition C is made；
(2) composition A is cooled to 45 DEG C, composition B is then added, stirs evenly；
(3) continue to be cooled to 40 DEG C or less obtained gels under stiring, composition C is added, stirs evenly, obtains bacteriostatic gel Agent.
Embodiment 2
0.3 part of kuh-seng root extract, 0.05 part of cnidium monnieri berry extract, 0.1 part of houttuynia extract, 0.3 part of Herba Saussureae Involueratae extract, 0.8 part of Fructus Kochiae extract, 0.05 part of peppermint oil, 0.3 part of cnidium oil, 0.05 part of rilanit special；Other are the same as embodiment 1.
Embodiment 3
0.05 part of kuh-seng root extract, 0.3 part of cnidium monnieri berry extract, 0.5 part of houttuynia extract, Herba Saussureae Involueratae extract 0.05 Part, 0.3 part of Fructus Kochiae extract, 0.3 part of peppermint oil, 0.05 part of cnidium oil, 0.3 part of rilanit special；Other are the same as embodiment 1.
The test result of the fungistatic effect of bacteriostatic gel described in 2 embodiment 1-3 of table
Embodiment 1 Embodiment 2 Embodiment 3
The bacteriostasis rate of Escherichia coli 97% 92% 92%
The bacteriostasis rate of staphylococcus aureus 95% 93% 91%
The bacteriostasis rate of Candida albicans 96% 91% 93%
Clinical testing data:
Choose 2 patients, using embodiment 1 prepare bacteriostatic gel, it is filling in disposable vaginal to being administered in gel device, often It is secondary to continue 30 days, specific example is as follows one time a day using 3.5g:
Test example 1: Nie；Female, 53 years old, bacterial infection, trichomonas, colpomycosis, vagina aging started to accelerate；
The course for the treatment of: from January 8th, 2018 by 2 8th, 2018, adhere to being administered with bacteriostatic gel daily；
Curative effect: foregoing conditions all significantly mitigate.Therapeutic index is 99.9%.
Test example 2: 38 years old, have there is the vaginas aging tendency disease such as pruritus of vagina, lower body peculiar smell, abnormal leucorrhea in Zhao, female Shape；
The course for the treatment of: from April 21,21 days to 2018 March in 2018, adhere to being administered with gelling agent daily；
Curative effect: foregoing conditions all significantly mitigate.Therapeutic index is 100%.

Claims (3)

1. a kind of bacteriostatic gel, which is characterized in that by weight, including following component:
0.05-0.3 parts of kuh-seng root extract, 0.05-0.3 parts of cnidium monnieri berry extract, 0.1-0.5 parts of houttuynia extract, saussurea involucrata 0.05-0.3 parts of flower extract, 0.3-0.8 parts of Fructus Kochiae extract, 0.05-0.3 parts of peppermint oil, 0.05-0.3 parts of cnidium oil, hydrogen Change castor oil 0.05-0.3 parts；
0.4-0.6 parts of ethyl alcohol, 0.1-0.2 parts of chlorhexidine acetate；
1-3 parts of hydroxyethyl cellulose, 4-6 parts of glycerol, 1-3 parts of propylene glycol, 88-90 parts of water.
2. bacteriostatic gel according to claim 1, which is characterized in that by weight, including following component:
0.1 part of kuh-seng root extract, 0.1 part of cnidium monnieri berry extract, 0.2 part of houttuynia extract, 0.1 part of Herba Saussureae Involueratae extract, 0.5 part of Fructus Kochiae extract, 0.1 part of peppermint oil, 0.1 part of cnidium oil, 0.1 part of rilanit special；
0.5 part of ethyl alcohol, 0.12 part of chlorhexidine acetate；
2 parts of hydroxyethyl cellulose, 5 parts of glycerol, 2 parts of propylene glycol, 89.08 parts of water.
3. a kind of preparation method of bacteriostatic gel as claimed in claim 1 or 2, which comprises the following steps:
(1) by hydroxyethyl cellulose, glycerol, mixed with propylene glycol, water is added after hydroxyethyl cellulose dissolution in stirring, and stirring adds Heat keeps the temperature 20 minutes to 95 DEG C, and composition A is made；
By kuh-seng root extract, cnidium monnieri berry extract, houttuynia extract, Herba Saussureae Involueratae extract, Fructus Kochiae extract, peppermint Oil, cnidium oil, rilanit special mixing, are made composition B；
Chlorhexidine acetate is dissolved in ethyl alcohol, composition C is made；
(2) composition A is cooled to 45 DEG C, composition B is then added, stirs evenly；
(3) continue to be cooled to 40 DEG C or less obtained gels under stiring, composition C is added, stirs evenly, obtains bacteriostatic gel Agent.
CN201910130070.1A 2019-02-21 2019-02-21 A kind of bacteriostatic gel and preparation method thereof Pending CN109602813A (en)

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CN112315900A (en) * 2020-11-06 2021-02-05 南京道大药业有限公司 Gel for nursing private parts of women
CN113304203A (en) * 2021-06-09 2021-08-27 广东快女生物技术有限公司 Formula of female private film capable of relieving itching and removing odor and preparation method thereof

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CN1883599A (en) * 2005-06-24 2006-12-27 广西花红药业有限责任公司 Chinese medicinal composition for treating women's episioitis or colpitis and method for preparing same
CN105997847A (en) * 2016-06-24 2016-10-12 昆明悟真生物科技有限公司 Anti-bacterial gel
CN106552078A (en) * 2016-12-02 2017-04-05 广州绿人生物科技有限公司 A kind of multifunctional biological agent
CN108452295A (en) * 2018-03-28 2018-08-28 丹东欣时代生物医药科技有限公司 A kind of compact gel and preparation method antibacterial, that adjust microecological balance

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Publication number Priority date Publication date Assignee Title
CN1883599A (en) * 2005-06-24 2006-12-27 广西花红药业有限责任公司 Chinese medicinal composition for treating women's episioitis or colpitis and method for preparing same
CN105997847A (en) * 2016-06-24 2016-10-12 昆明悟真生物科技有限公司 Anti-bacterial gel
CN106552078A (en) * 2016-12-02 2017-04-05 广州绿人生物科技有限公司 A kind of multifunctional biological agent
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112315900A (en) * 2020-11-06 2021-02-05 南京道大药业有限公司 Gel for nursing private parts of women
CN113304203A (en) * 2021-06-09 2021-08-27 广东快女生物技术有限公司 Formula of female private film capable of relieving itching and removing odor and preparation method thereof

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Application publication date: 20190412