CN113081928B

CN113081928B - Plant antibacterial gel and preparation method and application thereof

Info

Publication number: CN113081928B
Application number: CN202110471634.5A
Authority: CN
Inventors: 杨云仙
Original assignee: Yunnan Saisen Biotechnology Co ltd
Current assignee: Yunnan Saisen Biotechnology Co ltd
Priority date: 2021-04-29
Filing date: 2021-04-29
Publication date: 2023-06-06
Anticipated expiration: 2041-04-29
Also published as: CN113081928A

Abstract

The invention belongs to the technical field of antibiosis and disinfection, and particularly relates to a plant antibacterial gel and a preparation method and application thereof. The antibacterial gel provided by the invention takes fructus forsythiae extract as a main body, is matched with rhizoma bletillae extract, radix sophorae flavescentis extract, peony root extract, honeysuckle extract, cortex phellodendri extract, aloe juice and essential oil, and can disinfect skin without water under the condition of no water without adding ethanol and artificial fragrance under the action of auxiliary materials, and is mild and free of stimulation. According to the invention, through reasonable control of the amount, the components are mutually synergistic, so that the sterilization and bacteriostasis effects of the bacteriostasis gel are effectively improved; meanwhile, the antibacterial gel disclosed by the invention has the effects of moisturizing and protecting skin, relieving red swelling and itching pain of skin after mosquito bites, and is not easy to produce toxic and side effects.

Description

Plant antibacterial gel and preparation method and application thereof

Technical Field

The invention belongs to the technical field of antibiosis and disinfection, and particularly relates to a plant antibacterial gel and a preparation method and application thereof.

Background

Various microorganisms exist in the population life of people, and partial microorganisms can enter the human body to greatly influence the in-vivo environment of the human body and even cause various infectious diseases. Along with the development of society, people pay more and more attention to health, and various disinfection products are started to disinfect foods, bodies or clothes, for example, 75% ethanol is commonly used in medical institutions such as hospitals; chinese patent application No. 201210039685.1 describes a skin antibacterial agent comprising ginkgo leaf juice, white chrysanthemum juice, radix Puerariae juice, purslane juice, fistular onion stalk juice, mugwort leaf juice, white eggplant root juice, distilled water, etc.

However, the common problems in the existing disinfection products are: the ethanol concentration is high, the ethanol is accompanied with strong pungent smell, and partial skin sensitive people and infants can produce adverse reactions such as skin itching, redness and swelling and the like due to inadaptation; it also needs to be washed with water, and is not suitable for areas with serious water shortage or water resource shortage. Or has poor antibacterial and bacteriostatic effects and can not effectively clean the skin.

Disclosure of Invention

The invention aims to provide a plant antibacterial gel, which does not contain ethanol, can disinfect skin under the condition of no water, is mild and non-irritating, and has the effects of moisturizing and protecting skin.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides a plant antibacterial gel, which comprises fructus forsythiae extract, rhizoma bletillae extract, radix sophorae flavescentis extract, peony root extract, honeysuckle extract, cortex phellodendri extract, aloe juice, essential oil, auxiliary materials and water;

the plant antibacterial gel comprises, by 100g, 1-10 g of fructus forsythiae extract, 1-10 g of rhizoma bletillae extract, 1-10 g of radix sophorae flavescentis extract, 0.1-5 g of peony root extract, 0.1-5 g of honeysuckle extract, 0.1-5 g of cortex phellodendri extract, 1-10 g of aloe juice and 0.1-1 g of essential oil.

Preferably, the fructus forsythiae extract, rhizoma bletilla extract, radix sophorae flavescentis extract, peony root extract, honeysuckle extract and cortex phellodendri extract are alcohol extracts.

Preferably, the essential oil comprises one or more of pelargonium roseum oil, rosemary leaf oil, melaleuca alternifolia leaf oil, spearmint leaf oil and citronella leaf oil;

when the essential oil contains pelargonium roseum essential oil, the mass of the pelargonium roseum essential oil is 0.01-0.5 g in every 100g of plant antibacterial gel; when the essential oil contains rosemary leaf oil, the mass of the rosemary leaf oil is 0.01-0.5 g in every 100g of plant antibacterial gel; when the essential oil contains the intergrowth melaleuca alternifolia leaf oil, the mass of the intergrowth melaleuca alternifolia leaf oil is 0.01-0.5 g in every 100g of plant antibacterial gel; when the essential oil contains spearmint leaf oil, the mass of the spearmint leaf oil is 0.01-0.5 g in every 100g of plant antibacterial gel; when the essential oil contains citronella leaf oil, the mass of the citronella leaf oil is 0.01-0.5 g in every 100g of plant antibacterial gel.

Preferably, the auxiliary materials comprise a thickening agent, a solubilizer, an antioxidant, a pH regulator and a humectant;

the mass of the thickening agent is 0.1-0.6 g based on 100g of plant antibacterial gel; when the auxiliary material contains a solubilizer, the mass of the solubilizer is 0.1-1.5 g; when the auxiliary material contains antioxidant, the mass of the antioxidant is 0.1-0.6 g; when the auxiliary material contains the pH regulator, the mass of the pH regulator is 0.1-5 g; when the auxiliary material contains the humectant, the mass of the humectant is 0.5-5 g.

Preferably, the thickener comprises carbomers; the solubilizing agent comprises hydrogenated castor oil; the antioxidant comprises p-hydroxyacetophenone; the pH regulator comprises triethanolamine; the humectant comprises glycerin.

The invention also provides a preparation method of the plant antibacterial gel, which comprises the following steps: mixing fructus forsythiae extract, rhizoma bletilla extract, radix Sophorae Flavescentis extract, radix moutan root extract, flos Lonicerae extract, cortex Phellodendri extract, essential oil, adjuvants and water to obtain plant antibacterial gel.

Preferably, the invention relates to a mixture of fructus forsythiae extract, rhizoma bletillae extract, radix sophorae flavescentis extract, peony root extract, honeysuckle extract, cortex phellodendri extract, essential oil, auxiliary materials and water, which comprises the following steps:

mixing a thickener, a humectant and water to obtain a first premix;

mixing the antioxidant with the first premix to obtain a second premix;

mixing fructus forsythiae extract, rhizoma bletilla extract, radix Sophorae Flavescentis extract, radix moutan root extract, flos Lonicerae extract, cortex Phellodendri extract, aloe juice, and the second premix with pH regulator to obtain a third premix;

and mixing and emulsifying the essential oil, the solubilizer and the third premix to obtain the antibacterial gel.

The invention also provides application of the plant antibacterial gel in daily necessities.

the plant antibacterial gel comprises, by 100g, 1-10 g of fructus forsythiae extract, 1-10 g of rhizoma bletillae extract, 1-10 g of radix sophorae flavescentis extract, 1-10 g of peony root extract, 1-10 g of honeysuckle extract, 1-10 g of cortex phellodendri extract, 1-10 g of aloe juice and 0.1-1 g of essential oil.

The antibacterial gel provided by the invention takes fructus forsythiae extract as a main body, is matched with rhizoma bletillae extract, radix sophorae flavescentis extract, peony root extract, honeysuckle extract, cortex phellodendri extract, aloe juice and essential oil, and can disinfect skin without water under the condition of no water without adding ethanol and artificial fragrance under the action of auxiliary materials, and is mild and free of stimulation. Has effects of moistening skin, and relieving skin inflammation, itching and pain after mosquito bite. The essential oil also plays a role of the flavoring agent, does not need to add an artificially synthesized flavoring agent, and effectively improves the sterilization and bacteriostasis effects of the antibacterial gel through the mutual synergistic effect of the components by reasonably controlling the amount, thereby being suitable for wider crowds and not easy to generate toxic and side effects.

Furthermore, the invention utilizes ethanol to extract traditional Chinese medicine components to prepare the traditional Chinese medicine extract, well reserves the activity performance of each medicinal material, has good stability, has good killing effect on escherichia coli, candida albicans and staphylococcus aureus, and has sterilizing log values of more than 99.9 percent.

The antibacterial gel provided by the invention can be directly added into daily necessities such as hand sanitizer, shampoo, bath foam and the like, and can supplement skin moisture to keep the skin moist for a long time.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 shows the color change of a plant bacteriostatic gel sample stock solution after being placed in a constant temperature and humidity box with the temperature of 54 ℃ and the relative humidity of 85% for 0 day and 14 days.

Detailed Description

the antibacterial gel provided by the invention comprises 1-10 g of fructus forsythiae extract, preferably 2-8 g, and more preferably 6g, calculated by 100g of plant antibacterial gel. The fructus forsythiae extract is preferably a Hypericum perforatum extract. In the invention, the Hypericum perforatum has bitter, pungent and flat taste, can clear heat and detoxify, regulate menstruation and stop bleeding, contains various active ingredients such as volatile oil, forsythin ester and the like, and has good antibacterial effect.

The antibacterial gel provided by the invention comprises 1-10 g of fructus forsythiae extract, preferably 2-8 g, and more preferably 6g, calculated by 100g of plant antibacterial gel. The fructus forsythiae is preferably Hypericum perforatum. In the invention, the Hypericum perforatum has bitter, pungent and flat taste, can clear heat and detoxify, regulate menstruation and stop bleeding, contains various active ingredients such as volatile oil, forsythin ester and the like, and has good antibacterial effect.

The antibacterial gel provided by the invention comprises 1-10 g of bletilla striata extract, preferably 1-2 g, and more preferably 1g, calculated by 100g of plant antibacterial gel. In the invention, the bletilla striata is tuber of the Chinese traditional medicine named as bletilla striata Bletillae (Thunb.) Reichb.f. of the genus Bletillae of the family Orchidaceae, and has the effects of astringing, stopping bleeding, detumescence and promoting granulation. It is used for treating hemorrhage, carbuncle, swelling, pyocutaneous disease, rhagadia manus et pedis, and scald due to fire and water. Has remarkable inhibiting effect on human type tubercle bacillus and inhibiting effect on candida albicans and trichophyton cis.

The antibacterial gel provided by the invention comprises 1-10 g of kuh-seng extract, preferably 1-5 g, and more preferably 1g, calculated by 100g of plant antibacterial gel. In the invention, the radix sophorae flavescentis is the dried root of Sophora flavescens ait. Is used for treating damp-heat diarrhea, hematochezia, jaundice, damp-heat leukorrhagia, yin swelling and itching, eczema, skin itching, scabies, damp-heat urine. The bacillus tuberculosis, the bacillus dysenteriae, the staphylococcus aureus and the escherichia coli have the inhibiting effect and also have the inhibiting effect on various dermatophytes. Also has diuretic, antiinflammatory, antiallergic, tranquilizing, antiasthmatic, expectorant, leukocyte increasing, and antitumor effects.

Based on 100g of plant antibacterial gel, the antibacterial gel provided by the invention comprises 0.1-5 g of peony root extract, preferably 1-5 g, and more preferably 1g. In the invention, the peony root is the dried root bark of Paeonia suffruticosa Andr of Paeonia genus of Paeoniaceae family, and has the effects of clearing heat, cooling blood, promoting blood circulation and removing blood stasis. Mainly treats the toxic heat with spot, the blood heat with epistaxis, the warm disease with yin injury, yin deficiency with fever, night heat with early cool, no sweat with bone steaming, blood stagnation with amenorrhea and dysmenorrhea, traumatic injury, carbuncle with sore, has stronger anti-depression effect and a certain anti-influenza virus effect on staphylococcus albus, bacillus subtilis, escherichia coli, bacillus dysenteriae, typhoid bacillus, paratyphoid bacillus, proteus, pseudomonas aeruginosa, hemolytic streptococcus, pneumococcus, vibrio cholerae and the like.

Based on 100g of plant antibacterial gel, the antibacterial gel provided by the invention comprises 0.1-5 g of honeysuckle extract, preferably 1-5 g, and more preferably 1g. In the invention, the honeysuckle has the effects of clearing heat and detoxicating and dispelling wind and heat, and contains volatile oil, luteolin, inositol, flavonoids, inositol, saponin, tannins and the like. The separated chlorogenic acid and isochlorogenic acid have broad-spectrum antibacterial effect, have strong inhibition effect on pathogenic bacteria such as staphylococcus aureus, bacillus dysenteriae and the like, and also have inhibition effect on various pathogenic microorganisms such as leptospira, influenza virus, pathogenic mold and the like.

The antibacterial gel provided by the invention comprises 0.1-5 g of phellodendron bark extract, preferably 1-5 g, and more preferably 1g, calculated by 100g of plant antibacterial gel. In the present invention, huang Bai is bitter and cold in nature and is ascribed to kidney and bladder meridians. The cortex phellodendri has the functions of resisting bacteria, promoting bile flow, promoting urination, reducing blood pressure, relieving fever, reducing blood sugar and protecting blood platelets clinically, and can be used for treating sores and ulcers, eczema, wet sores and other diseases on the skin.

In the present invention, the fructus forsythiae extract, rhizoma bletilla extract, radix sophorae flavescentis extract, peony root extract, honeysuckle extract and cortex phellodendri extract are preferably alcohol extracts. The invention uses ethanol to extract, improves the extraction rate of medicinal materials, and well reserves the active ingredients of each medicinal material; the volume concentration of ethanol in the extraction process is preferably 50% to 80%, more preferably 50%. The mass ratio of the traditional Chinese medicine to the ethanol is preferably 1 (3-5), and is further preferably 1:3; the extraction temperature is preferably 80-90 ℃ during ethanol extraction; the ethanol extraction time is preferably 2 to 3 hours, more preferably 2 hours. The invention preferably extracts fructus forsythiae extract, rhizoma bletillae extract, radix sophorae flavescentis extract, peony root extract, honeysuckle extract and cortex phellodendri extract sequentially. The invention sequentially extracts the weeping forsythiae capsule, the bletilla tuber, the kuh-seng, the peony root, the honeysuckle and the phellodendron, and can avoid the problem of incomplete extraction caused by the saturation of the dissolution of the solute. The invention well reserves the activity of each medicinal material by extracting the active ingredients of fructus forsythiae, rhizoma bletillae, radix sophorae flavescentis, peony root, honeysuckle and phellodendron, has good stability, has good killing effect on escherichia coli, candida albicans and staphylococcus aureus, and has sterilizing log values of more than 99.9 percent.

In the invention, the dosage ratio of the weeping forsythia extract, the bletilla tuber extract, the kuh-seng extract, the peony root extract, the honeysuckle extract and the phellodendron bark extract is preferably 2-8 g: 1-5 g: 1-5 g: 1-5 g: 1-5 g:1 to 5g, more preferably 6g:1g:1g:1g:1g:1g.

The antibacterial gel provided by the invention comprises 1-10 g of aloe juice, preferably 1-5 g, and more preferably 1g, calculated by 100g of plant antibacterial gel. In the invention, the aloe juice can be used as a humectant besides the antibacterial effect.

Based on 100g of plant antibacterial gel, the antibacterial gel provided by the invention comprises 0.1-1 g of essential oil, and preferably one or more of pelargonium roseum oil, rosemary leaf oil, reciprocally-grown cajeput leaf oil, spearmint leaf oil and citronella leaf oil. When the essential oil contains pelargonium roseum oil, the mass of the pelargonium roseum oil is preferably 0.01-0.5 g, and more preferably 0.1g, based on 100g of the plant antibacterial gel; when the essential oil contains rosemary leaf oil, the mass of the rosemary leaf oil is preferably 0.01-0.5 g, more preferably 0.1g, based on 100g of plant antibacterial gel; when the essential oil contains the intergrowth melaleuca alternifolia leaf oil, the mass of the intergrowth melaleuca alternifolia leaf oil is preferably 0.01-0.5 g, and more preferably 0.1g, based on 100g of plant antibacterial gel; when the essential oil contains spearmint leaf oil, the mass of the spearmint leaf oil is preferably 0.01-0.5 g, more preferably 0.05g, based on 100g of plant antibacterial gel; when the essential oil contains citronella leaf oil, the mass of the citronella leaf oil is preferably 0.01-0.5 g, more preferably 0.05g, based on 100g of the plant antibacterial gel; the essential oil is further preferably a mixture of pelargonium roseum oil, rosemary leaf oil, reciprocally-grown cajeput leaf oil, spearmint leaf oil and citronella leaf oil, and the mass of the essential oil mixture is not more than 1g based on 100g of plant antibacterial gel. The mass ratio of the pelargonium roseum oil, the rosemary leaf oil, the reciprocally-grown melaleuca alternifolia leaf oil, the spearmint leaf oil and the citronella leaf oil is preferably 10:10:10:5:5. in the invention, the essential oil is mild and has no stimulation, the function of the flavoring agent is exerted, artificial synthetic flavor is not needed to be added, toxic and side effects are not easy to generate, and the application range is wide.

The antibacterial gel takes fructus forsythiae as a main body, and is matched with bletilla striata, peony root, kuh-seng, honeysuckle, phellodendron bark, aloe juice and essential oil, and the components are mutually synergistic through reasonable control, so that the sterilization and antibacterial effects of the antibacterial gel are effectively improved, and toxic and side effects are not easy to generate.

The antibacterial gel provided by the invention further comprises auxiliary materials such as a thickening agent, a solubilizer, an antioxidant, a pH regulator, a humectant and the like. In the invention, when the auxiliary material contains a thickening agent, the mass of the thickening agent is 0.1-0.6 g, preferably 0.45g, based on 100g of plant antibacterial gel; the thickener according to the invention is preferably a carbomer. In the present invention, the carbomer may be used as a gelling agent and an emulsion stabilizer in addition to a thickener.

In the present invention, when the auxiliary material contains a solubilizing agent, the solubilizing agent is 0.1 to 1.5g, preferably 0.6g, per 100g of the plant antibacterial gel. The solubilizing agent of the present invention is preferably hydrogenated castor oil. In the present invention, the hydrogenated castor oil may be used as an emulsifier in addition to the solubilizer.

In the invention, the mass ratio of the essential oil to the hydrogenated castor oil is preferably 4:10, so that the essential oil and the hydrogenated castor oil are fully dissolved.

In the present invention, when the auxiliary material contains an antioxidant, the antioxidant is 0.1 to 0.6g, preferably 0.1 to 0.5g, more preferably 0.2g, based on 100g of the plant antibacterial gel. The antioxidant of the present invention is preferably p-hydroxyacetophenone.

In the present invention, when the auxiliary material contains a pH regulator, the pH regulator is 0.1 to 5g, preferably 0.1 to 0.5g, more preferably 0.4g, based on 100g of the plant antibacterial gel. The pH regulator is preferably triethanolamine.

In the present invention, when the auxiliary material contains a humectant, the humectant is 0.5 to 15g, preferably 1 to 10g, and more preferably 4g, based on 100g of the plant antibacterial gel. The humectant of the present invention is preferably glycerin.

The water according to the invention is preferably purified water; the invention has no special requirements on the source of the purified water.

The antibacterial gel provided by the invention contains fructus forsythiae extract, rhizoma bletillae extract, cortex moutan extract, radix sophorae flavescentis extract, honeysuckle extract, cortex phellodendri extract, aloe juice and essential oil, and can be used for disinfecting skin under the condition of no water without adding artificially synthesized flavoring agent and ethanol under the combined action of a thickening agent, a solubilizer, an antioxidant, a pH regulator and a humectant, and is mild and non-irritating, so that the antibacterial gel is widely applicable to human populations.

The invention also provides a preparation method of the antibacterial gel, which comprises the following steps: mixing fructus forsythiae extract, rhizoma bletilla extract, radix Sophorae Flavescentis extract, radix moutan root extract, flos Lonicerae extract, cortex Phellodendri extract, essential oil, aloe juice, adjuvants and water to obtain fructus forsythiae washing-free antibacterial gel. In the invention, the mixing mode of the weeping forsythia extract, the bletilla tuber extract, the kuh-seng extract, the peony root extract, the honeysuckle extract, the phellodendron bark extract, the essential oil, the aloe juice, the auxiliary materials and the water preferably comprises the following steps of:

mixing a thickener, a humectant and water to obtain a first premix;

mixing the antioxidant with the first premix to obtain a second premix;

mixing fructus forsythiae extract, rhizoma bletilla extract, radix Sophorae Flavescentis extract, radix moutan root extract, flos Lonicerae extract, cortex Phellodendri extract, aloe juice, the second premix and pH regulator to obtain a third premix;

In the present invention, the mixture of the thickener, the humectant and the water is preferably mixed in an emulsifying pot; further preferably, the thickener, the humectant and the water are put into an emulsifying pot in this order, and the thickener, the humectant and the water are dispersed in cold water and stirred. In the present invention, the temperature of the cold water is preferably 20 to 37 ℃, and more preferably 25 ℃; the stirring speed is preferably 1500rpm to 2000rpm, more preferably 2500rpm; the stirring time is preferably 20 to 30 minutes, more preferably 30 minutes. The thickening agent, water and humectant are sequentially added to enable the thickening agent to be fully fused; the cold water is dispersed and stirred, so that the thickener, the humectant and the water are fully hydrated, and the full fusion of the mixed solution is promoted.

In the invention, after the stirring is finished, the temperature is increased to carry out second stirring, so that the fusion of all components in the first premix liquid is promoted; the temperature of the second stirring is preferably 50-80 ℃, and more preferably 60 ℃; the speed of the second stirring is preferably 2000rpm to 3000rpm, more preferably 2500rpm; the second stirring time is preferably 20 to 30 minutes, more preferably 30 minutes.

After the first premix is obtained, the antioxidant is preferably mixed when the temperature of the first premix is reduced to 35 to 50 ℃ or lower, more preferably 45 ℃ or lower. In the present invention, the mixing of the antioxidant and the first premix is preferably stirring mixing; the stirring and mixing time is preferably 10 to 20 minutes, more preferably 10 minutes.

After the second premix is obtained, the invention preferably mixes the fructus forsythiae extract, the rhizoma bletillae extract, the radix sophorae flavescentis extract, the peony root extract, the honeysuckle extract and the phellodendron extract, the second premix, the aloe juice and the pH regulator. In the present invention, the mixing is performed under stirring conditions; in the invention, the Hypericum perforatum extract, bletilla tuber extract, radix sophorae flavescentis extract, peony root extract, honeysuckle extract, phellodendron bark extract and aloe juice are added into the second premix in sequence, and then are stirred and mixed, and then the pH regulator is added. The invention further preferably reduces the temperature of the second premix to 40-50 ℃, and more preferably adds the traditional Chinese medicine extract and aloe juice when reducing to 40 ℃. In the invention, the traditional Chinese medicine extract and aloe juice are added at low temperature, so as to protect the integrity of active ingredients.

The invention preferably adds a pH regulator into the mixture of the traditional Chinese medicine extract, the aloe juice and the second premix to obtain a third premix; in the present invention, the pH of the third premix is preferably 4.5 to 6.5, more preferably 6.21.

After the third premix is obtained, the essential oil, the solubilizer and the third premix are mixed and emulsified to obtain antibacterial gel; the solubilizing agent is capable of promoting dissolution of the essential oil with the third premix. According to the invention, pelargonium roseum oil, rosemary leaf oil, reciprocally-grown cajeput leaf oil, spearmint leaf oil, lemon citronella leaf oil and hydrogenated castor oil are sequentially added into the third premix, and mixed and solubilized under stirring conditions to obtain an essential oil solubilization liquid. In the present invention, the mixing solubilization is preferably performed under heating conditions; the heating temperature is preferably 40 to 45 ℃, and more preferably 40 ℃.

The invention preferably adds the essential oil solubilizing liquid into the third premix to realize mixed emulsification; the mixing and emulsifying are preferably carried out under stirring conditions; the stirring time is preferably 30 to 60 minutes, more preferably 30 minutes.

The invention also provides application of the antibacterial gel in daily necessities. When the antibacterial gel is used for preparing daily necessities, the antibacterial gel can be directly added into daily necessities such as hand sanitizer, shampoo, bath foam and the like, and skin moisture is supplemented, so that the skin can be moisturized for a long time; the antibacterial gel preferably accounts for 5-20% of the mass of the antibacterial daily necessities, and more preferably 15%.

For further explanation of the present invention, the following describes in detail a fructus forsythiae leave-in antibacterial gel, its preparation method and application provided by the present invention in connection with examples, but they should not be construed as limiting the scope of protection of the present invention.

The percentages mentioned in the following examples, unless otherwise indicated, are the proportions by mass of the respective components in the bacteriostatic gel.

Example 1

1) Sequentially weighing 100g of fructus forsythiae, 100g of radix sophorae flavescentis, 100g of peony root, 100g of rhizoma bletillae, 100g of honeysuckle and 100g of cortex phellodendri, and respectively placing into 6 5L round bottom bottles;

2) Adding 3L of 50% -80% ethanol water solution into 6 round bottom bottles respectively, reflux extracting for 2h at 60 ℃, filtering to obtain an extracting solution and recovering ethanol;

3) Adding 3-5% glycerol to the extractive solution to assist dissolution, and fixing volume to 3L to obtain fructus forsythiae extract, radix Sophorae Flavescentis extract, radix moutan extract, rhizoma Bletillae extract, flos Lonicerae extract and cortex Phellodendri extract.

Example 2

1) Sequentially weighing 480g of Hypericum perforatum, 60g of radix sophorae flavescentis, 60g of peony root, 10g of rhizoma bletillae, 10g of honeysuckle and 10g of cortex phellodendri, mixing, pulverizing coarse powder, sieving with a 60-mesh medicine sieve, and placing in a 5L round bottom bottle;

2) Adding 2L of 50% ethanol into a round bottom bottle, reflux extracting for 2h, filtering to obtain mixed extract, and concentrating under reduced pressure until no ethanol smell exists;

3) Adding 3% glycerol to the extractive solution for dissolving, and fixing volume to 3L for use.

Example 3

Sequentially weighing and mixing 0.1g of pelargonium roseum oil, 0.1g of rosemary leaf oil, 0.1g of mutually-grown cajeput leaf oil, 0.05g of spearmint leaf oil, 0.05g of citronella leaf oil and 0.6g of hydrogenated castor oil to obtain an essential oil increasing solution.

Example 4

1) Accurately weighing 6g of fructus forsythiae extract, 1g of radix sophorae flavescentis extract, 1g of peony root extract, 1g of rhizoma bletillae extract, 1g of honeysuckle extract and 1g of cortex phellodendri extract in the embodiment 1 for standby;

2) Adding 0.45g carbomer, 2g glycerin and 83.15g purified water into an emulsifying pot in sequence, dispersing cold water, stirring at a high speed, fully hydrating, starting a heating system, and stirring for 20-30 min at a constant temperature of 60 ℃;

3) Cooling to below 45 ℃, adding 0.2g of p-hydroxyacetophenone, and continuously stirring for 10min;

4) Sequentially adding the Hypericum perforatum extract, rhizoma bletilla extract, radix Sophorae Flavescentis extract, radix moutan root extract, flos Lonicerae extract, cortex Phellodendri extract and 2g aloe juice obtained in step 1) to 40 deg.C, stirring for 10min, and adding 0.2g triethanolamine;

5) Mixing 0.1g of pelargonium roseum oil, 0.1g of rosemary leaf oil, 0.1g of mutually-grown cajeput leaf oil, 0.05g of spearmint leaf oil, 0.05g of citronella leaf oil and 0.6g of hydrogenated castor oil in sequence, heating at 40 ℃, and stirring for 5min till complete transparency to obtain a solubilized essential oil composition;

6) And (3) putting the solubilized essential oil composition into an emulsifying pot, and stirring for 30min to obtain the plant antibacterial gel.

Example 5

The same as in example 4 except that in step 1), 4g of fructus forsythiae extract, 2g of radix Sophorae Flavescentis extract, 2g of peony root extract, 1g of rhizoma bletilla extract, 1g of flos Lonicerae extract and 1g of cortex Phellodendri extract were accurately weighed in example 1.

Example 6

The procedure is as in example 4, except that the stirring conditions in step 2) are 80℃and 3000rpm.

Example 7

The same as in example 4, except that step 5) mixing 0.2g of the reciprocally-grown melaleuca alternifolia leaf oil, 0.1g of the spearmint leaf oil, 0.1g of the geranium oil and 0.6g of the hydrogenated castor oil in sequence, heating at 40 ℃, stirring for 5min to be completely transparent, obtaining a solubilized essential oil composition;

comparative example 1

1) Taking 1.0kg of dry Hypericum perforatum, pulverizing, adding 4kg of 50% ethanol water solution, refluxing at 80-90 ℃ for 3 hours, and filtering to obtain filtrate;

2) Concentrating the filtrate under reduced pressure until no alcohol smell exists, to obtain alcoholic suspension of fructus forsythiae.

3) Adding 3% glycerol to the alcoholic suspension of fructus forsythiae for dissolving, and fixing volume to 5L with pure water to obtain herba Hyperici perforati extract.

Comparative example 2

The same procedure as in example 4 was repeated except that the extract of Forsythia suspensa, sophora flavescens, paeonia suffruticosa root, bletillae rhizoma, lonicera japonica, phellodendron amurense, aloe juice and essential oil were not contained to obtain an adjuvant mixture.

Test example 1

Bacteriostasis experiment 1

Sampling: 50g of Hypericum perforatum extract in comparative example 1 as sample No. 1; taking 50g of mixed extract obtained by mixing fructus forsythiae, radix sophorae flavescentis, peony root, rhizoma bletillae, honeysuckle and cortex phellodendri in example 2, and taking the mixed extract as a sample No. 2; mixing 60g of fructus forsythiae extract, 10g of radix sophorae flavescentis extract, 10g of peony root extract, 10g of rhizoma bletillae extract, 10g of honeysuckle extract and 10g of cortex phellodendri extract in example 1 to obtain a sample No. 3; 50g of the essential oil solubilizing liquid in example 3 was taken as sample No. 4; 50g of the auxiliary material mixture in the comparative example 2 is taken as a blank matrix (namely a control); taking the prepared carriers in the step 2) to be respectively soaked in the samples 1-4 and the blank matrix (control group), taking out the carriers by using sterile forceps when the carriers are respectively acted for 20min, respectively transferring the carriers into test tubes containing 5.0g of PBS, uniformly mixing the carriers, sampling 1.0g after proper dilution, and calculating the colony numbers of viable bacteria of the samples 1-4 and the control group, wherein the viable bacteria count results are shown in the following tables 1-4:

table 11 sample killing conditions (CFU/mL) of different test bacteria

Note that: in the table "/" indicates that the sample does not have the capability of killing the corresponding test bacteria

From the data in Table 1, it can be seen that the stock solution, the 2-fold dilution and the 4-fold dilution of sample No. 1 can obviously inhibit the growth of Escherichia coli and Staphylococcus aureus, the killing rate of Escherichia coli and Staphylococcus aureus is more than 99.9%, and the inhibition effect on Salmonella and Candida albicans is not achieved.

Table 22 sample killing conditions (CFU/mL) of different test bacteria

As can be seen from the data in table 2, the stock solution of sample No. 2 can obviously inhibit the growth of escherichia coli, staphylococcus aureus, salmonella and candida albicans, and the killing rate of escherichia coli, staphylococcus aureus, salmonella and candida albicans is more than 99.9%; however, the 2-fold dilution and the 4-fold dilution of sample No. 2 do not inhibit the growth of Escherichia coli, staphylococcus aureus, salmonella and Candida albicans.

Table 3 3 sample killing conditions (CFU/mL) of different test bacteria

As can be seen from the data in table 3, the stock solution of sample No. 3 can obviously inhibit the growth of escherichia coli, staphylococcus aureus, salmonella and candida albicans, and the killing rate of escherichia coli, staphylococcus aureus, salmonella and candida albicans is more than 99.9%; the 2-fold diluent of the sample No. 3 only has remarkable inhibition effect on salmonella, the killing rate on salmonella is 100%, and the growth of escherichia coli, staphylococcus aureus and candida albicans cannot be inhibited; sample No. 3, 4-fold dilution, had no inhibitory effect on the growth of E.coli, staphylococcus aureus, salmonella and Candida albicans.

Table 4 4 sample effect on killing of different test bacteria (CFU/mL)

As can be seen from the data in table 4, stock solutions, 2-fold dilutions and 4-fold dilutions of sample No. 4 were able to significantly inhibit the growth of escherichia coli, staphylococcus aureus, salmonella and candida albicans.

Bacteriostasis experiment 2

1) 50g of the plant antibacterial gel of example 4 is taken as a sample No. 5; 50g of the adjuvant mixture of comparative example 2 was used as a blank matrix (i.e., control);

2) Taking the prepared carrier in the step 1) to be respectively soaked in a No. 5 sample and a blank matrix (control group), respectively acting for 2min, 5min, 10min and 20min, taking out the carrier by using sterile forceps, respectively transferring the carrier into a test tube containing 5.0g of PBS, uniformly mixing, sampling for 3 times after proper dilution, sampling for 1.0g each time, and repeating the test; the number of viable bacteria colonies of the sample stock solution No. 5 and the control group was calculated, and the viable bacteria count results were as follows in tables 5 to 8:

table 5 5 antibacterial results of sample stock solution on Candida albicans

Note that: the sheet was a laboratory flat glass bottle.

As can be seen from the data in table 5, the stock solution of sample No. 5 has a significant inhibitory effect on the growth of candida albicans. The candida albicans growth can be completely inhibited after 2min of action, and the inhibiting effect is quite obvious after 20min of duration. The antibacterial gel prepared by the invention has good inhibition effect on candida albicans and long antibacterial duration.

Table 6 5 antibacterial results of sample stock solution on Staphylococcus aureus

Note that: the tablet is a plate glass bottle for experiments

As can be seen from the data in table 6, the stock solution of sample No. 5 has a significant inhibitory effect on the growth of staphylococcus aureus. The growth of staphylococcus aureus with the concentration of more than 98 percent can be inhibited after the action is carried out for 2 minutes, the antibacterial effect is gradually enhanced along with the extension of the action time, and the inhibition effect is quite obvious after the action lasts for 20 minutes. The antibacterial gel prepared by the invention has good inhibition effect on staphylococcus aureus and long antibacterial duration.

Table 75 antibacterial results of sample stock solution on E.coli

Note that: the tablet is a plate glass bottle for experiments

As can be seen from the data in table 7, the stock solution of sample No. 5 has a significant inhibitory effect on the growth of escherichia coli. The growth of more than 98 percent of escherichia coli can be inhibited after the action is performed for 2 minutes, the inhibiting effect reaches 100 percent after 5 minutes, and the inhibiting effect is quite obvious after the action is performed for 20 minutes. The antibacterial gel prepared by the invention has good inhibition effect on escherichia coli and long antibacterial duration.

Table 85 antibacterial results of sample stock solution on Salmonella

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Note that: the tablet is a plate glass bottle for experiments

As can be seen from the data in table 8, stock solution of sample No. 5 has a significant inhibitory effect on salmonella growth. The growth of the escherichia coli can be inhibited by more than 100% after the action is performed for 2min, the inhibiting effect reaches 100% after 5min, and the inhibiting effect is quite obvious after the action is performed for 20 min. The antibacterial gel prepared by the invention has good inhibition effect on escherichia coli and long antibacterial duration.

Test example 2

The treatment was carried out in the same manner as in test example 1, and after the completion of the mixing, sample No. 5 was sealed as it is and stored in a constant temperature and humidity cabinet at a temperature of 54℃and a relative humidity of 85% for 14 days.

Stability experiment 1

The stability performance of the sample No. 5 for inhibiting candida albicans is tested according to the appendix C of GB15979-2002 of the hygienic Standard of disposable hygienic products under the environment that the temperature is 21.9-23.2 ℃ and the relative humidity is 53-57%. Specific test results are shown in table 9 and fig. 1:

table 9 5 sample stock solution stability test results

As can be seen from the data in Table 9, the average antibacterial rate of the antibacterial gel sample provided by the invention on candida albicans after being subjected to the action of 2min, 5min, 10min and 20min in a constant temperature and humidity box with the original temperature of 54 ℃ and the relative humidity of 85% for 14 days is 100.0%, which proves that the antibacterial gel is good in stability.

The left side of the figure 1 is a plant antibacterial gel sample prepared by 8 months and 21 days in 2020; the right side is the color change condition of the sample after the left side plant antibacterial gel sample stock solution is placed in a constant temperature and humidity box with the temperature of 54 ℃ and the relative humidity of 85% for 14 days. As can be seen from fig. 1, compared with 54 ℃, the antibacterial gel sample after being placed for 0 days at 85% relative humidity for 14 days (9 months and 3 days in 2020) has no color change and good stability.

Stability experiment 2

Determination of pH range for sample No. 5 using a broad pH test paper

The pH meter was turned on to be in a stable state, the pH meter was washed according to the instructions of use, the pH meter was calibrated (positioned) with a mixed phosphate buffer solution at ph=6.86, the washing electrode was wiped dry, the second calibration (positioning) was performed with potassium hydrogen phthalate at ph=4.01, then the electrode was washed with distilled water, the electrode was wiped dry with filter paper, and then the pH value of sample No. 5 was measured. The process was repeated 2 times to obtain an average value. The test results are shown in Table 10 below:

table 10 sample No. 5 pH measurement results

Note that: the pH stabilizing range is 4.5-6.5

As can be seen from Table 10, the sample of No. 5 plant antibacterial gel is in a constant temperature and humidity box with the original temperature of 54 ℃ and the relative humidity of 85% for 14 days, and the pH value is in the pH range of No. 5 sample before storage, which shows that the antibacterial gel has good stability.

Test example 3

Safety experiment 1

Test samples the same as in test example 2, the concentrations of lead, arsenic and mercury contained in the samples were determined using a one-ten-thousandth balance (EBO 2-03) and an inductively coupled plasma mass spectrometer (EA 01-01) in accordance with the cosmetic safety Specification, 2015, under an environment having a temperature of 25.2 ℃ and a relative humidity of 54.0%, and the results of the measurements are shown in table 11 below:

table 11 sample stock lead, arsenic and mercury determination results

Note that: safe usage of lead: <10mg/kg; safe use amount of arsenic: <2mg/kg; the safe dosage of mercury is less than 1mg/kg.

As can be seen from Table 11, the content of lead in the antibacterial gel sample of the present invention is <0.03mg/kg, the content of arsenic is 0.0017mg/kg, and the content of mercury is <0.001mg/kg, and the antibacterial gel of the present invention is safe in use within the safe dosage range.

Safety experiment 2

Under the environment of 22.4 ℃ and 56.0% of relative humidity, according to the appendix B of the disposable sanitary articles Standard GB15979-2002, inoculating the antibacterial gel of the sample No. 5 into nutrient agar culture mediums (batch No. 1083841), a Sai culture medium (batch No. 1083101) and the like, and utilizing a biochemical incubator (EB 29-01); mould incubator (EB 30-01); biosafety cabinet (EC 18-01), and after 14 days of culture, the growth of bacteria in the culture medium is detected. The specific test results are shown in Table 12 below:

table 12 sample stock solution microbiological determination results

As can be seen from Table 12, after 14 days of culture, no microorganisms were detected, and the antibacterial gel of the present invention was safe to use.

Test example 4

Skin irritation test

Detecting a sample: sample stock solution No. 5

The detection method comprises the following steps: 3 white New Zealand rabbits (male 2, female 1; A, B and C respectively) with a weight of 2.0kg-2.5kg are subjected to hair removal on both sides of the back of the New Zealand rabbits for 24 hours before the test, wherein the hair removal areas on both the left side and the right side are about 3cm multiplied by 3cm, the left side is taken as an application area, and the right side is taken as a control area. Measuring 0.5g of the test substance (the sample stock solution No. 5 is coated on the coating area, the normal saline is coated on the control area) respectively, directly dripping the test substance onto the skin of the animal coating area, covering the test substance with a layer of non-stimulated plastic film, and fixing the test substance with a non-stimulated adhesive tape, wherein the application time is 4 hours. After application for 4 hours, the application area was rinsed with warm water to remove residues. The application was once daily for 14 days. The local skin response was observed 24h after each application, the average score (stimulation index) per day was calculated for each animal (average score per day = total score of erythema and edema for all animals tested 14 d/number of animals tested x 14), and skin irritation intensity was determined in the "disinfection technical Specification" 2002 edition tables 2-12. The specific detection results are shown in the following table 13:

table 13 No. 5 sample antibacterial gel multiple complete skin irritation experiment result

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As can be seen from Table 13, the skin of all the test animals had no erythema, edema and other abnormalities during the 14d observation period after the contamination; the skin of the control zone of the tested animal has no abnormal phenomenon during the observation period. The average integral of the stimulation response per animal per day (stimulation index) was 0. The antibacterial gel has no stimulation to skin.

Test example 5

Ethanol content determination experiment

According to GB/T26373-2020 annex A of alcohol disinfectant health requirement, ethanol detection is carried out on No. 5 sample antibacterial gel, and the detection result is shown in the following table 14

Table 14 sample stock solution microbiological determination results

As can be seen from Table 14, the antibacterial gel of the present invention does not contain ethanol.

Test example 6

Vaginal mucosa irritation test

Detecting a sample: sample stock solution No. 5 as test solution

The detection method comprises the following steps: the 6 female white New Zealand rabbits were randomly divided into an experimental group and a control group, each group being 3. A blunt end hose with a length of about 8cm was connected to a 2g syringe, and the test solution was filled for use. One set was prepared for each animal. The animals were held on their backs, exposing the perineum and vaginal orifice. The catheter was gently inserted into the vagina (4-5 cm) after being wetted with the test solution, and 2g of the test solution was slowly injected by a syringe, and the catheter was withdrawn to complete the contamination. 24 hours after the infection, the animals are sacrificed by an air embolism method, the complete vagina is taken out by laparotomy, the vagina is longitudinally cut, and whether congestion, edema and the like appear is observed visually. Then the vagina is put into 10% formalin solution for fixation for more than 24 hours, tissue preparations of the two ends and the central 3 parts of the vagina are selected, after HE staining, histopathological examination is carried out, and the vaginal mucosa stimulating response is scored according to the vaginal mucosa stimulating response scoring standard in the disinfection technical Specification (2002 edition) 2.3.5. The specific test results are shown in Table 15 below:

table 15 results of sample No. 5 vaginal mucosa irritation test

As can be seen from Table 15, in the vaginal mucosa irritation test, the results of pathological examination showed that the vaginal mucosa irritation index of the test animals was-0.66. In the experimental process, the ingestion, drinking, urination and defecation, behavioural activities and the like of the New Zealand rabbits in the control group and the experimental group are not abnormal. After the two groups of animals are killed respectively after 24 hours of contamination, the vaginal mucosa of all the tested animals in the experimental group is basically complete through naked eyes, and congestion, edema and other abnormal manifestations are not seen; the vaginal mucosa of all animals in the control group was intact. The antibacterial gel provided by the invention has no obvious irritation to the vaginal mucosa of experimental animals.

From the above examples, it can be seen that the present invention provides a bacteriostatic gel that is not only free of ethanol, but also can be used for skin disinfection in the absence of water; has mild effect, no irritation to skin and vaginal mucosa, safety, no adverse side effect, and moisture keeping and skin caring effects.

Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, it should be understood that other embodiments may be devised in accordance with the present embodiments without departing from the spirit and scope of the invention.

Claims

1. The plant antibacterial gel is characterized by comprising, based on 100g of the plant antibacterial gel, 6g of fructus forsythiae extract, 1g of rhizoma bletillae extract, 1g of radix sophorae flavescentis extract, 1g of peony root extract, 1g of honeysuckle extract, 1g of cortex phellodendri extract, 2g of aloe juice, 0.1g of geranium oil, 0.1g of rosemary leaf oil, 0.1g of mutually-generated cajeput leaf oil, 0.05g of spearmint leaf oil, 0.05g of citronella leaf oil, 0.6g of hydrogenated castor oil, 0.45g of carbomer, 2g of glycerol, 0.2g of p-hydroxyacetophenone, 0.2g of triethanolamine and 83.15g of water;

the fructus forsythiae extract, rhizoma bletillae extract, radix sophorae flavescentis extract, peony root extract, honeysuckle extract and cortex phellodendri extract are alcohol extracts;

the preparation method of the alcohol extract comprises the following steps:

mixing the traditional Chinese medicine materials with 50% -80% ethanol aqueous solution according to 100g: mixing 3L of materials in a mass-volume ratio, extracting at 60 ℃ under reflux for 2 hours, and filtering to recover ethanol to obtain an extract;

adding 3-5% of glycerol to the extracting solution for dissolution, and fixing the volume to 3L to obtain the alcohol extract;

the preparation method of the plant antibacterial gel comprises the following steps: sequentially adding carbomer, glycerol and water into an emulsifying pot, dispersing cold water, stirring at a high speed, fully hydrating, starting a heating system, and stirring for 20-30 min at a constant temperature of 60 ℃;

cooling to below 45 ℃, adding the p-hydroxyacetophenone, and continuously stirring for 10min;

sequentially adding the fructus forsythiae extract, rhizoma bletilla extract, radix Sophorae Flavescentis extract, radix moutan extract, flos Lonicerae extract, cortex Phellodendri extract and aloe juice, stirring for 10min, and adding triethanolamine;

mixing the pelargonium roseum oil, rosemary leaf oil, reciprocally-grown melaleuca alternifolia leaf oil, spearmint leaf oil, lemon citronella leaf oil and hydrogenated castor oil in sequence, heating at 40 ℃, and stirring for 5min to be completely transparent to obtain a solubilized essential oil composition;

and (3) putting the solubilized essential oil composition into an emulsifying pot, and stirring for 30min to obtain the plant antibacterial gel.

2. The method for preparing a plant antibacterial gel according to claim 1, wherein the plant antibacterial gel is obtained by mixing forsythia extract, bletilla striata extract, kuh-seng extract, peony root extract, honeysuckle extract, phellodendron bark extract, aloe juice, geranium oil, rosemary leaf oil, reciprocally white thousand leaf oil, spearmint leaf oil, citronella leaf oil, hydrogenated castor oil, carbomer, glycerin, p-hydroxyacetophenone, triethanolamine and water;

the mixing comprises the following steps:

mixing carbomer, glycerol and water to obtain a first premix;

mixing the p-hydroxyacetophenone with the first premix to obtain a second premix;

mixing fructus forsythiae extract, rhizoma bletilla extract, radix Sophorae Flavescentis extract, radix moutan root extract, flos Lonicerae extract, cortex Phellodendri extract, aloe juice, and the second premix with triethanolamine to obtain a third premix;

and mixing and emulsifying pelargonium roseum oil, rosemary leaf oil, intergrowth cajeput leaf oil and spearmint leaf oil, citronella leaf oil, hydrogenated castor oil and the third premix to obtain the plant antibacterial gel.

3. The use of the plant antibacterial gel of claim 1 or the plant antibacterial gel prepared by the preparation method of claim 2 in daily necessities.