CN111437225A

CN111437225A - Hemostatic, granulation-promoting and antibacterial composition and application thereof

Info

Publication number: CN111437225A
Application number: CN202010325619.5A
Authority: CN
Inventors: 江桂忠; 戴丽芳
Original assignee: Guangdong Zhenghuiyuan Industrial Co ltd
Current assignee: Guangdong Zhenghuiyuan Industrial Co ltd
Priority date: 2020-04-23
Filing date: 2020-04-23
Publication date: 2020-07-24

Abstract

The invention relates to the technical field of antibacterial washing and caring products, and particularly provides a hemostatic, granulation-promoting and antibacterial composition, which at least comprises the following raw materials: chitosan, plant extracts; the weight ratio of the chitosan to the plant extract is 1: (2-12); the plant extract at least comprises one of nakedflower beautyberry extract, honeysuckle extract, lightyellow sophora root extract, amur corktree bark extract, indigowoad root extract, dandelion extract, plantain herb extract, Chinese angelica extract, motherwort herb extract, common cnidium fruit extract and grapefruit extract. The invention adopts various raw materials which are rich in the functions of clearing heat, dispelling wind, relieving sore throat, resisting inflammation, resisting bacteria and easing pain, and the raw materials supplement each other, can clear heat and detoxify, stop bleeding, promote tissue regeneration and improve the immune function of a user, thereby achieving the effects of resisting virus, resisting bacteria, diminishing inflammation and resisting virus.

Description

Hemostatic, granulation-promoting and antibacterial composition and application thereof

Technical Field

The invention relates to the technical field of antibacterial washing and caring products, in particular to a hemostatic and granulation-promoting antibacterial composition and application thereof.

Background

Along with the development of society and the improvement of the living standard of people, people pay more and more attention to the sanitation of working and living environments for the self health requirement, and various antibacterial products are produced. The oral cavity is the entrance of the respiratory digestive tract, has important physiological functions of chewing, swallowing, speech expression and the like, is one of important ways for pathogenic microorganisms to invade the organism, has proper temperature and humidity in the oral cavity, and is the earliest contact with flora (bacteria and fungi) from the air, thereby providing superior conditions for the growth and the propagation of the microorganisms. When the immunity of the organism is low, endogenous or exogenous infection is easy to occur, and the importance of oral care is particularly obvious. In addition, the gynecological diseases of the siblings of the vast females are always the obstacles which disturb the life of the females, and the gynecological symptoms such as pruritus, peculiar smell, pain, cachexia, aging, abnormal complexion, chloasma, old fastness, infertility and the like are always troubled the females for hundreds of years, and for the adult females, the most common diseases are not cold but genital tract infection; the most common uncomfortable symptoms are not fever, cough, headache and the like, but pudendum pruritus, peculiar smell, pain, frequent micturition, urgent micturition and the like. The long-term application of a certain chemical can easily cause the drug resistance of bacteria, and the chemical has irritation and corrosivity, has toxic and side effects, and more importantly, lacks of real green and environment-friendly safety.

The plant extracts from natural sources have rich biological functions, such as emulsification, bacteriostasis, deodorization, hemostasis, anti-inflammation, and prevention of diseases such as dental caries and oral ulcer, and the plant active extracts with the functions are reasonably and effectively used in a composite way, so that unexpected effects can be achieved. Because the plant active molecules have strong synergistic effect, after two or more than two extracts are mixed according to a certain proportion, the biological characteristics of the respective active molecules can be fully exerted to the utmost extent, and the effect which cannot be compared with the effect of using one extract singly is achieved, namely the additive effect.

Excellent performances of chitosan such as biological functionality and compatibility, blood compatibility, safety, microbial degradability and the like are widely concerned by various industries, and the chitosan has wide application in various fields such as medicine, food, chemical engineering, cosmetics, water treatment, metal extraction and recovery, biochemistry, biomedical engineering and the like, is convenient to use, can be quickly sterilized, and has wide application prospect and great market value. Wherein the joint use of chitosan and antibacterial ions is disclosed in patent CN102552964B, a nano-silver chitosan composite antibacterial composition, a wound plaster and a preparation method thereof; CN101721733A also discloses a medical chitosan antibacterial dressing. Chitosan and antibacterial particles are widely applied to antibacterial materials, particularly various fiber fabrics, but the problem of weak antibacterial ability, poor effect and the like of chitosan in some washing and caring articles is also existed, so that the chitosan is ignored by people.

Disclosure of Invention

In order to solve the above technical problems, the present invention provides a hemostatic, granulation-promoting and antibacterial composition, which at least comprises the following raw materials: chitosan, plant extracts; the weight ratio of the chitosan to the plant extract is 1: (2-12); the plant extract at least comprises one of nakedflower beautyberry extract, honeysuckle extract, lightyellow sophora root extract, amur corktree bark extract, isatis root extract, dandelion extract, plantain herb extract, Chinese angelica extract, motherwort extract, common cnidium fruit extract and grapefruit extract.

As a preferred technical solution, the raw material of the present invention further comprises silver ions; the content of the silver ions is 0.02-0.15 wt% of the chitosan.

As a preferred technical solution, the raw material of the present invention further comprises silver ions; the content of the silver ions is 0.05-0.1 wt% of the chitosan.

As a preferred technical solution, the weight ratio of the chitosan to the plant extract in the invention is 1: (6-10).

As a preferred technical solution, the plant extract in the present invention comprises a combination of beautyberry extract, isatis root extract, dandelion extract and plantain extract; the weight ratio of the callicarpa nudiflora extract, the isatis root extract, the dandelion extract and the plantain extract is 1: (0.5-1): (0.1-1): (0.5-1.5).

As a preferred technical solution, the plant extract in the present invention comprises a combination of beautyberry extract, honeysuckle extract, sophora flavescens extract and phellodendron amurense extract; the weight ratio of the beautyberry extract to the honeysuckle extract to the sophora flavescens extract to the phellodendron amurense extract is 1: (0.5-1.5): (0.5-1.5): (0.5-1.5).

The second aspect of the invention provides an application of the hemostatic, granulation-promoting and antibacterial composition, which is applied to one of washing products, textiles, non-woven fabrics, clothes, bedding products, household products and medical products.

As a preferable technical scheme, the agent of the washing and caring product is selected from at least one of antibacterial liquid, antibacterial gel, antibacterial soft capsule, antibacterial hard capsule, antibacterial tablet, dispersing agent, antibacterial cream, antibacterial emulsion and antibacterial spray.

The third aspect of the invention provides an antibacterial liquid formulation, which comprises the following components in percentage by weight: 5-20% of the hemostatic, myogenic and antibacterial composition, and purified water to make up the balance.

In a fourth aspect, the invention provides an application of the washing and caring product, which is applied to at least one of nasal cavity, oral cavity, throat, surface skin, human genitals, upper and lower respiratory tracts, lung and anorectum.

Compared with the prior art, the invention has the following beneficial effects:

the invention provides a hemostatic, granulation-promoting and antibacterial composition, which can be applied to the preparation of various cleaning and nursing articles with different dosages according to requirements. In addition, the application range of the various preparation washing and nursing products prepared by the invention is wider, the products can be suitable for infection of various bacteria, fungi, viruses and the like of nasal cavities, oral cavities, throats, surface skins, human genitals, upper and lower respiratory tracts, lungs, anorectums and the like, can quickly sterilize, deeply and multiply, promote wound healing, have no toxic or side effect, are convenient to use, have stable sterilization effect and take effect quickly.

Drawings

FIG. 1 is a flow chart of the process for extracting Callicarpa nudiflora.

FIG. 2 is a flow chart of the process for extracting flos Lonicerae extract.

FIG. 3 is a flow chart of the process of the Sophora flavescens Aiton extract.

FIG. 4 is a flow chart of the process for preparing the extract of phellodendron amurense.

Detailed Description

The technical features of the technical solutions provided by the present invention will be further clearly and completely described below with reference to the specific embodiments, and it should be apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

The words "preferred", "preferably", "more preferred", and the like, in the present invention, refer to embodiments of the invention that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention.

The invention provides a hemostatic, myogenic and antibacterial composition, which at least comprises the following raw materials: chitosan, plant extracts; the weight ratio of the chitosan to the plant extract is 1: (2-12); the plant extract at least comprises one of nakedflower beautyberry extract, honeysuckle extract, lightyellow sophora root extract, amur corktree bark extract, indigowoad root extract, dandelion extract, plantain herb extract, Chinese angelica extract, motherwort herb extract, common cnidium fruit extract and grapefruit extract.

In some preferred embodiments, the weight ratio of chitosan to plant extract is 1: (6-10).

In some more preferred embodiments, the weight ratio of chitosan to plant extract is 1: 8.

in some embodiments, the feedstock further comprises silver ions; the content of the silver ions is 0.02-0.15 wt% of the chitosan.

In some preferred embodiments, the feedstock further comprises silver ions; the content of the silver ions is 0.05-0.1 wt% of the chitosan.

In some more preferred embodiments, the feedstock further comprises silver ions; the content of the silver ions is 0.08 wt% of the chitosan.

In the invention, the CAS number of the silver ions is 7761-88-8, the raw material grade is pharmaceutical grade, and the purity is 99%.

The invention provides a hemostatic and tissue regeneration antibacterial composition, which has very beneficial effect of deep and rapid sterilization, and the inventor finds that particularly the compounding of silver ions, chitosan and specific plant extracts can generate excellent synergistic effect, and improves the sterilization effect of the antibacterial composition on deep bacteria, and the inventor thinks that the chitosan is a high molecular substance with certain bioadhesion, wherein the slow release effect of the silver ions in the antibacterial liquid can be enhanced after the chitosan is compounded with the silver ions, the chitosan can be more easily adsorbed to the application surface after being used on sensitive viscous skin such as human surface skin or oral mucosa, the detention time of the chitosan on the application part can be prolonged, and the chitosan and cells have special effect, and contain more hydroxyl and amino groups, so as to promote the opening of the upper epidermal cell channel, the skin can be promoted to rapidly absorb active substances, and bacterial and virus in deep layers can be thoroughly eliminated, so that the effect is remarkable.

In some embodiments, the plant extract comprises at least one of beautyberry extract, honeysuckle extract, sophora flavescens extract, phellodendron amurense extract, isatis root extract, dandelion extract and plantain extract.

In some embodiments, the plant extract comprises a combination of beautyberry extract, isatis root extract, dandelion extract, plantain extract; the weight ratio of the callicarpa nudiflora extract, the isatis root extract, the dandelion extract and the plantain extract is 1: (0.5-1): (0.1-1): (0.5-1.5).

In some preferred embodiments, the weight ratio of the beautyberry extract, the isatis root extract, the dandelion extract and the plantain extract is 1: 0.6: 0.8: 1.

in some embodiments, the plant extract comprises a combination of beautyberry extract, honeysuckle extract, sophora flavescens extract, phellodendron amurense extract; the weight ratio of the beautyberry extract to the honeysuckle extract to the sophora flavescens extract to the phellodendron amurense extract is 1: (0.5-1.5): (0.5-1.5): (0.5-1.5).

In some preferred embodiments, the weight ratio of the beautyberry extract, the honeysuckle extract, the sophora flavescens extract and the phellodendron amurense extract is 1: 1: 1: 1.

the invention is suitable for preventing and nursing infection of nasal cavity, oral cavity and throat, various acute and chronic rhinitis, pharyngitis, otitis externa, oral ulcer and other infections of ear, nose, throat and throat, and promotes the healing of wound surface. Is suitable for the surface skin, mucosa, damaged epidermis and mucosa wound surface of human body, and has hemostatic, granulation promoting, antibacterial and anti-inflammatory effects; meanwhile, the product has pathogenic microorganism effects of staphylococcus aureus, hemolytic streptococcus, pseudomonas aeruginosa, escherichia coli, antifungal, antivirus and the like.

The invention can be prepared into corresponding dosage according to different requirements of use parts, can be applied to genital organs, accessories, anorectal infection and the like of men and women (female vagina), has the effects of resisting bacteria, diminishing inflammation, resisting syphilis Luospira (Ag ion neonatal eye plum infection), resisting fungi, viruses, pathogenic microorganisms (Ag resists more than 6 microorganisms) and the like, and has stable and long-acting effect of resisting bacteria and viruses. It can also be used for treating upper and lower respiratory tract infection, pulmonary bacterial infection, fungal infection, and viral infection.

Chitosan

Chitosan (chitosan) chitin N-deacetylated product, chitin, chitosan, cellulose have close chemical structure, cellulose is hydroxyl at C2 position, chitin, chitosan are replaced by an acetamido and amino at C2 position respectively, chitin and chitosan have biodegradability, cell affinity and biological effect etc. many unique properties, especially chitosan containing free amino, are the only basic polysaccharide in natural polysaccharide. The amino group in the chitosan molecular structure has stronger reactivity than the acetamido group in the chitin molecule, so that the polysaccharide has excellent biological function and can carry out chemical modification reaction. Therefore, chitosan is considered to be a functional biomaterial with greater potential for use than cellulose.

The raw material grade of the chitosan is pharmaceutical grade, and the purity of the raw material is 99.5%.

Callicarpa nudiflora extract

Callicarpa nudiflora (academic name: Callicarpa nudiflora hook. et Arn.) is Verbenaceae, and can reach 7 m; old branches have no hair and obvious skin holes, and branches with dense grayish brown color and hairy hair of twigs, petioles and inflorescences. The leaves are oval and oblong in shape and are coated with needles, the surfaces of the leaves are dark green, the leaves become black after being dried, the polyspora rosea is developed, and the bracts are linear or coated with needles; calyx, corolla purple or pink, anther oval, tiny, nearly spherical, red, flowering in 6-8 months, and bearing fruit in 8-12 months. The callicarpa nudiflora leaf has the effects of stopping bleeding, relieving pain, removing blood stasis and reducing swelling. Treating traumatic hemorrhage, traumatic injury swelling and pain, rheumatic swelling and pain, pulmonary tuberculosis hemoptysis, and gastrointestinal hemorrhage; the research on the chemical components and pharmacological action of Callicarpa nudiflora shows that the Callicarpa nudiflora extract mainly contains flavone, tannin, triterpenes, phenols and other components, wherein the total flavone extract has the effects of resisting thrombosis, reducing the viscosity of whole blood, inhibiting platelet aggregation, resisting inflammation, resisting bacteria and the like.

In the invention, the callicarpa nudiflora extract can be obtained commercially or by self-making; preferably, the callicarpa nudiflora extract of the invention is prepared by the preparation method shown in figure 1 of the invention.

In some embodiments, the detailed preparation method of the callicarpa nudiflora extract in fig. 1 is as follows:

weighing Callicarpa nudiflora raw materials, crushing, screening by a 20-60 mesh sieve, adding a proper amount of 55% ethanol for precipitation, carrying out ultrasonic crushing and suction filtration to obtain filtrate, discharging, evaporating to obtain a crude product, adding 75% methanol for dissolution, subpackaging and warehousing.

In the present invention, the detailed preparation method of the callicarpa nudiflora extract is a technical method well known to those skilled in the art.

In the invention, the quality requirement of the callicarpa nudiflora extract prepared according to the preparation method shown in fig. 1 meets the requirements according to the detection result of the drug standard of the ministry of health in 2010 edition.

In the present invention, the sensory physical and chemical indexes of the callicarpa nudiflora extract prepared according to fig. 1 are shown in table 1.

TABLE 1 sensory and physicochemical indices of Callicarpa nudiflora extract

In the present invention, the microorganism content index of the extract of callicarpa nudiflora prepared according to fig. 1 is shown in table 2.

TABLE 2 microbial content index

Project (project)	Standard (standard)
		Total bacterial colony count (cfu/ml)	≤200
Total fungal colony count (cfu/ml)	≤100
		Escherichia coli	Should not detect
Hemolytic streptococcus	Should not detect
		Pseudomonas aeruginosa	Should not detect
Staphylococcus aureus	Should not detect

The invention adds certain nakedflower beautyberry leaf extract which mainly contains flavone, tannin, triterpenes, phenols and other components, wherein the active components such as the total flavone extract can obviously improve the effects of bacteriostasis, hemostasis, anti-inflammation, analgesia and the like of the antibacterial liquid. The inventor further finds that the antibacterial effect of the system can be further improved and the hemostatic effect is better enhanced after the compound of the callicarpa nudiflora extract, silver ions and chitosan is added, the inventor thinks that the chitosan can form gel substances in the application process probably due to the biological functionality, compatibility and blood compatibility of the chitosan, and the gel substances have more active ingredients in the callicarpa nudiflora extract, and can play a certain physical action and a certain chemical action on bleeding parts to a certain extent, so that the hemostatic effect is more obvious, and the gel substances can also provide a remarkable lasting antibacterial and antivirus effect on wound practical parts.

Honeysuckle extract

The honeysuckle is a rod-shaped, thick upper part and thin lower part, slightly bent, 2-3cm long, 3mm upper part diameter and 1.5mm lower part diameter, yellow white or green white surface, dense coating, short molhair, chlorogenic acid (chlorogenacic acid) and luteolin (L uteolin) as main active ingredients, the chlorogenic acid is widely present in plants, the content of honeysuckle and eucommia bark is high, and the chlorogenic acid has wide pharmacological action.

In the invention, the honeysuckle extract can be obtained commercially or by self-preparation; preferably, the honeysuckle extract is prepared by the preparation method shown in figure 2.

In some embodiments, the detailed preparation method of the lonicera flos extract in fig. 2 is as follows:

weighing honeysuckle raw materials, crushing, screening by a 20-60-mesh sieve, adding a proper amount of 75% ethanol for precipitation, adding 9 times of purified water for boiling for 2.5 hours, adding an equivalent amount of 80% ethanol for standing for 24 hours, extracting supernate, removing ethanol, subpackaging and warehousing to obtain the honeysuckle health-care tea.

In the present invention, the detailed preparation method of the honeysuckle extract is a technical method well known to those skilled in the art.

In the invention, the product quality requirement of the honeysuckle extract meets the specification according to the detection result of the 2010 version of Chinese pharmacopoeia of the drug standard of the ministry of health.

In the invention, the sensory physical and chemical indexes of the honeysuckle extract are shown in table 3.

Table 3 organoleptic and physicochemical indices of the honeysuckle extract

Project (project)	Standard (standard)
		Traits	Colorless or yellowish liquid
Smell(s)	No abnormal odor or peculiar smell
		Density of	1.002～1.031
Refraction of light	1.212～1.243
		Arsenic content	≤3(mg/kg)
Heavy metals	＜10ppm
		Content (wt.)	The solid content is more than 10 percent
Chlorogenic acid	17％～28％
		pH value	4.0～8.0

In the present invention, the index of the microorganism content of the honeysuckle extract is shown in table 4.

TABLE 4 microbiological content index of honeysuckle extract

Sophora flavescens extract

Radix Sophorae Flavescentis is dried root of radix Sophorae Flavescentis of Leguminosae, collected in spring and autumn, removed root head and branch root, cleaned, dried, or sliced and dried. It is bitter and cold. Has the functions of clearing heat, drying dampness, killing parasites and promoting urination. Can be used for treating dysentery with heat, hematochezia, jaundice, anuria, leucorrhea with red and white discharge, pudendal swelling, pudendal pruritus, eczema, skin pruritus, scabies, tinea, leprosy, and trichomonas vaginitis. The radix Sophorae Flavescentis extract is an alkaloid extracted from Sophoraflavicens (Sophoraflavicens) belonging to Sophora of Leguminosae. The raw material has tough root, coarse fibrous cross section and yellow-white color. Light smell, extremely bitter taste. The radix Sophorae Flavescentis extract contains matrine (matrine), oxymatrine (oxymatrine), oxymatrine (sodium-anol), N-methylceupatorine (N-methylcytisine), anagerine (anagyrine), bap-ifinoline (bap-ifinoline), dehydromatrine (Sophorcarpine), d-isokurarine (d-isomatrine), kuraridine (kurarid-in), norkurarinone (norkurarinone), kurarinol (kurarinol), neokurarinol (neo-kurarinol), norkurarinol (norkurarinol), isokurarinone, and formononetin (formononetin).

In the invention, the sophora flavescens extract can be obtained commercially or by self-preparation; preferably, the sophora flavescens ait extract is prepared by the preparation method shown in fig. 3.

In some embodiments, the detailed preparation method of the sophora flavescens extract in fig. 3 is as follows:

weighing radix Sophorae Flavescentis, pulverizing, sieving with 20-60 mesh sieve, adding 65% ethanol for precipitation, filtering to obtain filtrate, adding Tween 80 water solution to obtain radix Sophorae Flavescentis extract, packaging, and storing.

In the present invention, the detailed preparation method of the sophora flavescens extract is a technical method well known to those skilled in the art.

In the invention, the quality requirement of the sophora flavescens extract meets the specification according to the detection result of the 2010 version of Chinese pharmacopoeia of the drug standard of the ministry of health.

In the present invention, the sensory physical and chemical indexes of the sophora flavescens extract are shown in table 5.

Table 5 shows the sensory physical and chemical indexes of the Sophora flavescens extract

In the present invention, the index of the microbial content of the sophora flavescens extract is shown in table 6.

Index of microbial content of Sophora flavescens Aiton extract shown in Table 6

Cortex Phellodendri extract

Phellodendron amurense (hu a ngb oa), a dried bark of the phellodendron chinense schneid. It is commonly called Chuan Huang Bai. Peeling the bark, removing the coarse bark, and drying in the sun. The cortex Phellodendri extract is extracted from dried bark of phellodendron amurense or phellodendron amurense belonging to Rutaceae. The functions and indications are as follows: clear heat and dry dampness, purge fire and remove steam, remove toxicity and cure sore. Can be used for treating dysentery due to damp-heat pathogen, jaundice, leukorrhagia, stranguria caused by heat pathogen, tinea pedis, hectic fever due to yin-deficiency, night sweat, spermatorrhea, pyocutaneous disease, toxic swelling, eczema, and pruritus. Salted cortex phellodendri is used for nourishing yin and reducing internal heat. Can be used for treating hyperactivity of fire due to yin deficiency, night sweat and steaming bone.

In the invention, the phellodendron extract can be obtained by purchase or self-made; preferably, the phellodendron extract of the present invention is prepared by the preparation method shown in fig. 4 of the present invention.

In some embodiments, the detail preparation method of phellodendron amurense extract in fig. 4 is as follows:

weighing cortex Phellodendri, pulverizing, sieving with 20-60 mesh sieve, adjusting pH to 2, heating to 80 deg.C for 30 min, vacuum filtering, precipitating with distilled water, adjusting pH to 7, adding equal amount of 95% ethanol, vacuum filtering, adjusting pH to 2, heating to 80 deg.C for 30 min, vacuum filtering, dissolving precipitate with ethanol solution, adjusting pH to 4, and packaging.

In the present invention, the detailed preparation method of the phellodendron amurense extract is a technical method well known to those skilled in the art.

In the invention, the quality requirement of the phellodendron extract meets the specification according to the detection result of the 2010 version of Chinese pharmacopoeia of the drug standard of the ministry of health.

In the present invention, the sensory and physicochemical indices of the phellodendron amurense extract are shown in table 7.

The sensory physical and chemical indexes of phellodendron amurense extract shown in Table 7

Project (project)	Standard (standard)
		Traits	Colorless or yellowish liquid
Smell(s)	Extremely bitter taste
		Density of	1.039～1.051
Refraction of light	1.531～1.535
		Arsenic content	≤3(mg/kg)
Heavy metals	＜10ppm
		Content (wt.)	The solid content is more than 10 percent
Berberine	10％～30％
		pH value	4.0～8.0

In the present invention, the indices of the microorganism content of the phellodendron amurense extract are shown in Table 8.

Index of microbial content of phellodendron extract described in Table 8

In the invention, the inventor further finds that the honeysuckle extract, the sophora flavescens extract and the phellodendron bark extract are compounded with the callicarpa nudiflora extract, the chitosan and the silver ions, and can further generate a remarkable synergistic effect, so that the beauty-care tea has excellent effects of quickly sterilizing and stopping bleeding, and also has excellent effects of promoting granulation and tightening skin. After the four plant extracts are compounded with the chitosan and the silver ions, the chitosan gel has biocompatibility, can simultaneously improve the skin barrier repairing effect of active ingredients in the plant extracts, promotes the regeneration effect of collagen, and has the effect of promoting tissue regeneration rapidly.

Isatis root extract

The isatis root is root of Isatistinctoria L of Brassicaceae and Isatis tinctoria fort, or root and stem of Baphiicacanthus cusia (Nees) Brem of Acanthaceae, the root of Isatis tinctoria contains indinin (Indoxyl- β -glucoside), Isatin (Isatisn), Indigo (Indigo), Isatis tinctoria B (Isatan B), Uridine (Uridine), Uracil (Uracil), Hypoxanthine (Hypoxanthine), salicylic acid (Salicylicid), Qingdainone (Qingdainone), Daucosterol, β -Sitosterol (β -Sitosterol), Isatin B, propylin and butin, the plant protein, resin, saccharide, sinapside, amino acids and trace elements, the substance containing positive and negative bacteria, the substance containing anti-bacteria, the substance containing anthraquinones, β, the extract containing anthraquinones, anthralin and the extract having remarkable action on bacillus coli, staphylococcus aureus and staphylococcus.

The source of the isatis root extract is not particularly limited, and any one of the isatis root extracts which is generally commercially available can be used in the present invention.

Dandelion extract

Dandelion whole herb contains taraxasterol, choline, inulin, pectin and the like, roots contain taraxasterol, ψ -megestrol, taraxasterol, β -coumarol, stigmasterol, β -sitosterol, choline, organic acids, fructose, sucrose, glucose, glucoside and resins, rubber and the like, leaves contain lutein, orometin, phylloquinone, flowers contain arninodiol, lutein and luteolin, pollen contains β -sitosterol, 5 z-stigmast-7-en-3 β -ol, folic acid and vitamin C, green calyxin contains phylloquinone in green calyx, flower stems contain β -sitosterol and β -coumaronol.

Plantago asiatica extract

Plantain (plantaginiatica L inn.) is a plantago (plantaginiacee) plantago (pchemibarbita tago) plant, namely plantain, carrageen, plantain herb, swertia, floating, horse shake, cow enuresis, oxtongue, plantain, lichen, toad skin and the like.

The invention provides an application of the hemostatic and myogenic antibacterial composition, which is applied to one of washing products, textiles, non-woven fabrics, clothes, bedding products, household products and medical products.

In some embodiments, the personal care product has an agent property selected from at least one of an antibacterial liquid agent, an antibacterial gel agent, an antibacterial soft capsule, an antibacterial hard capsule, an antibacterial tablet, a dispersing agent, an antibacterial cream, an antibacterial emulsion, and an antibacterial spray.

In some preferred embodiments, the antibacterial liquid formulation comprises the following components in percentage by weight: 8-15% of the hemostatic, granulation-promoting and antibacterial composition, and purified water to make up the balance.

In some more preferred embodiments, the antimicrobial liquid formulation comprises, in weight percent: 10 percent of the hemostatic, myogenic and antibacterial composition and purified water to make up the balance.

According to a fourth aspect of the invention, the application of the washing and caring product is provided, and the washing and caring product is applied to at least one of nasal cavities, oral cavities, throats, surface skin, human genitals, upper and lower respiratory tracts, lungs and anorectal tracts.

The present invention is described in detail below by way of examples, and it should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention.

Example 1

A hemostatic, granulation-promoting and antibacterial composition at least comprises the following raw materials: chitosan, plant extracts; the weight ratio of the chitosan to the plant extract is 1: 8. the raw material also comprises silver ions; the content of the silver ions is 0.08 wt% of the chitosan.

The plant extract comprises the combination of beautyberry extract, honeysuckle extract, sophora flavescens extract and phellodendron extract; the weight ratio of the beautyberry extract to the honeysuckle extract to the sophora flavescens extract to the phellodendron amurense extract is 1: 1: 1: 1.

the beautyberry extract described in this example was prepared by the preparation method shown in fig. 1 of the present invention. The honeysuckle extract is prepared by the preparation method shown in figure 2. The sophora flavescens extract is prepared by the preparation method shown in figure 3. The phellodendron extract is prepared by the preparation method shown in figure 4.

An antibacterial liquid formulation comprises the following components in percentage by weight: 10 percent of the hemostatic, myogenic and antibacterial composition and purified water to make up the balance.

Example 2

A hemostatic, granulation-promoting and antibacterial composition at least comprises the following raw materials: chitosan, plant extracts; the weight ratio of the chitosan to the plant extract is 1: 6. the raw material also comprises silver ions; the content of the silver ions is 0.05 wt% of the chitosan.

The plant extract comprises the combination of beautyberry extract, honeysuckle extract, sophora flavescens extract and phellodendron extract; the weight ratio of the beautyberry extract to the honeysuckle extract to the sophora flavescens extract to the phellodendron amurense extract is 1: 0.5: 0.5: 0.5.

An antibacterial liquid formulation comprises the following components in percentage by weight: 8 percent of the hemostatic, granulation-promoting and antibacterial composition and purified water to make up the balance.

Example 3

A hemostatic, granulation-promoting and antibacterial composition at least comprises the following raw materials: chitosan, plant extracts; the weight ratio of the chitosan to the plant extract is 1: 10. the raw material also comprises silver ions; the content of the silver ions is 0.1 wt% of the chitosan.

The plant extract comprises the combination of beautyberry extract, honeysuckle extract, sophora flavescens extract and phellodendron extract; the weight ratio of the beautyberry extract to the honeysuckle extract to the sophora flavescens extract to the phellodendron amurense extract is 1: 1.5: 1.5: 1.5.

An antibacterial liquid formulation comprises the following components in percentage by weight: 15 percent of the hemostatic, myogenic and antibacterial composition and the balance of purified water.

Example 4

The plant extract comprises the combination of a callicarpa nudiflora extract, an isatis root extract, a dandelion extract and a plantain extract; the weight ratio of the callicarpa nudiflora extract, the isatis root extract, the dandelion extract and the plantain extract is 1: 0.6: 0.8: 1.

the beautyberry extract described in this example was prepared by the preparation method shown in fig. 1 of the present invention.

Example 5

The plant extract comprises the combination of honeysuckle extract, sophora flavescens extract and phellodendron amurense extract; the weight ratio of the honeysuckle extract to the sophora flavescens extract to the phellodendron amurense extract is 1: 1: 1.

the honeysuckle extract is prepared by the preparation method shown in figure 2. The sophora flavescens extract is prepared by the preparation method shown in figure 3. The phellodendron extract is prepared by the preparation method shown in figure 4.

Example 6

The plant extract comprises a combination of callicarpa nudiflora extract and honeysuckle extract; the weight ratio of the callicarpa nudiflora extract to the honeysuckle extract is 1: 1.

Example 7

A hemostatic, granulation-promoting and antibacterial composition at least comprises the following raw materials: chitosan, plant extracts; the weight ratio of the chitosan to the plant extract is 1: 2. the raw material also comprises silver ions; the content of the silver ions is 0.08 wt% of the chitosan.

Example 8

The plant extract comprises the combination of centella extract, honeysuckle extract, sophora flavescens extract and phellodendron extract; the weight ratio of the centella extract to the honeysuckle extract to the sophora flavescens extract to the phellodendron amurense extract is 1: 1: 1: 1.

Example 9

A hemostatic, granulation-promoting and antibacterial composition at least comprises the following raw materials: chitosan, plant extracts; the weight ratio of the chitosan to the plant extract is 1: 8. the raw material also comprises silver ions; the content of the silver ions is 5 wt% of the chitosan.

Example 10

The plant extract comprises the combination of beautyberry extract, honeysuckle extract, sophora flavescens extract and phellodendron extract; the weight ratio of the beautyberry extract to the honeysuckle extract to the sophora flavescens extract to the phellodendron amurense extract is 1: 0.1: 0.1: 4.

Examples11

A hemostatic, granulation-promoting and antibacterial composition at least comprises the following raw materials: chitosan, silver ions; the content of the silver ions is 0.08 wt% of the chitosan.

Performance testing

The antibacterial liquid agents prepared in examples 1 to 11 were subjected to the following various performance tests.

1. Sensory requirements: the sample was poured out in a small amount into a clear glass and observed at a light inspection site. It is judged whether the criteria as required in table 8 below are met.

And (3) testing results: examples 1-11 all meet the requirements of table 8.

TABLE 8 sensory requirements

Item	Index (I)
		Color and luster	Meet the specified color and luster
Form of the composition	Liquid, method for producing the same and use thereof
		Smell(s)	No peculiar smell or conforming to the regulated smell
Impurities	No visible impurities

2. Microorganism indexes are as follows: the method is carried out according to the method specified in GB15979-2002, and whether the standard meets the requirements of the following table 9 is judged.

And (3) testing results: examples 1 to 11 all meet the requirements of table 9.

TABLE 9 microbiological indicator requirements

Item	Index (I)
		Total bacterial colony count (cfu/ml)	≤200
Total fungal colony count (cfu/ml)	≤100
		Escherichia coli	Should not detect
Hemolytic streptococcus	Should not detect
		Pseudomonas aeruginosa	Should not detect
Staphylococcus aureus	Should not detect

3. The sterilization effect is as follows: the determination was made according to the method specified in the Disinfection Specification 2002 edition, in which it was determined whether the index requirements of Table 10 were met.

And (3) testing results: as shown in table 10-1.

TABLE 10 germicidal effectiveness index requirements

TABLE 10-1 test results of bactericidal effect

Examples	Sterilizing effect
		Example 1	Conform to
Example 2	Conform to
		Example 3	Conform to
Example 4	Conform to
		Example 5	Is not in compliance with
Example 6	Is not in compliance with

The sterilization effect of the antibacterial liquid prepared in the embodiment 1 is more prominent, and the sterilization rates of the antibacterial liquid prepared in the embodiment 1 on escherichia coli for 2min, 5min, 10min and 20min are 93.97%, 99.82%, 99.99% and 99.99% respectively; the sterilization rate of the staphylococcus aureus for 2min, 5min, 10min and 20min is 38.41%, 41.45%, 50.31% and 93.22% respectively; the sterilization rate of the pseudomonas aeruginosa for 2min, 5min, 10min and 20min is 97.33%, 99.99% and 99.99% respectively.

4. And (3) stability test: the determination was made according to the method specified in the Disinfection Specification 2002 edition, in which it was determined whether or not the index requirements of Table 11 were met.

And (3) testing results: as shown in table 11-1.

TABLE 11 index requirements

Time/bacterial species	Stock solution for 2min	Stock solution for 5min	Stock solution for 10min	Stock solution for 20min
					Candida albicans	＞99.99％	＞99.99％	＞99.99％	＞99.99％

TABLE 11-1 test results

5. The requirement of toxicological safety: the determination was made according to the method specified in the "Disinfection Specification" 2002 edition of the Ministry of health, in which it was determined whether or not the criteria of Table 12 were met.

And (3) testing results: examples 1-10 all meet the requirements of table 12.

TABLE 12 toxicological requirements

Item	Index (I)
		Skin irritation test	Without or with slight irritation
Acute oral toxicity test	Is practically nontoxic
		Acute eye irritation test	Has no irritation
Multiple complete skin irritation tests	Without or with slight irritation
		Irritation test of vaginal mucous membrane (multiple times)	No irritation to vaginal mucosa

6. The content of lead, arsenic and mercury is determined according to the method specified in the safety technical specification 2015 edition of cosmetics, wherein the indexes are judged to be met, and the indexes comprise the lead content of less than or equal to 30mg/kg, the arsenic content of less than or equal to 5mg/kg and the mercury content of less than 1 mg/L.

And (3) testing results: the examples 1 to 10 all meet the lead, arsenic and mercury content index requirements.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in other forms, and any person skilled in the art may modify or change the technical content disclosed above into an equivalent embodiment with equivalent changes, but all those simple modifications, equivalent changes and modifications made on the above embodiment according to the technical spirit of the present invention still belong to the protection scope of the present invention.

Claims

1. The hemostatic, myogenic and antibacterial composition is characterized by comprising the following raw materials: chitosan, plant extracts; the weight ratio of the chitosan to the plant extract is 1: (2-12); the plant extract at least comprises one of nakedflower beautyberry extract, honeysuckle extract, lightyellow sophora root extract, amur corktree bark extract, indigowoad root extract, dandelion extract, plantain herb extract, Chinese angelica extract, motherwort herb extract, common cnidium fruit extract and grapefruit extract.

2. The hemostatic, myogenic, and antibacterial composition of claim 1, wherein said source material further comprises silver ions; the content of the silver ions is 0.02-0.15 wt% of the chitosan.

3. The hemostatic, myogenic, and antibacterial composition of claim 2, wherein said source material further comprises silver ions; the content of the silver ions is 0.05-0.1 wt% of the chitosan.

4. The hemostatic, myogenic, and antibacterial composition of claim 1, wherein the weight ratio of chitosan to plant extract is 1: (6-10).

5. The tissue-regenerating antibacterial composition as claimed in any one of claims 1 to 4, wherein said plant extract comprises a combination of beautyberry extract, isatis root extract, dandelion extract, plantain extract; the weight ratio of the callicarpa nudiflora extract, the isatis root extract, the dandelion extract and the plantain extract is 1: (0.5-1): (0.1-1): (0.5-1.5).

6. The hemostatic, myogenic, and antibacterial composition of any one of claims 1-4, wherein said plant extract comprises a combination of beautyberry extract, honeysuckle extract, sophora flavescens extract, phellodendron amurense extract; the weight ratio of the beautyberry extract to the honeysuckle extract to the sophora flavescens extract to the phellodendron amurense extract is 1: (0.5-1.5): (0.5-1.5): (0.5-1.5).

7. Use of a hemostatic, myogenic, and antibacterial composition according to any one of claims 1-6 in one of a washing, textile, nonwoven, garment, bedding, household, and medical care product.

8. The use of the hemostatic, myogenic, and antibacterial composition of claim 7, wherein the lotion is at least one selected from the group consisting of an antibacterial liquid, an antibacterial gel, an antibacterial soft capsule, an antibacterial hard capsule, an antibacterial tablet, a dispersion, an antibacterial cream, an antibacterial emulsion, and an antibacterial spray.

9. The antimicrobial liquor of claim 8, wherein the components comprise, in weight percent: 5-20% of a hemostatic, myogenic, antibacterial composition as claimed in any one of claims 1-6, purified water to make up the balance.

10. Use of the cleaning composition of claim 8 in at least one of the nasal cavity, oral cavity, throat, superficial skin, human genitalia, upper and lower respiratory tract, lung, and anorectum.