CN112022947A - Bacteriostatic agent based on traditional Chinese herbal medicines and small molecular compounds, and preparation method and application thereof - Google Patents

Abstract

The invention relates to a bacteriostatic agent based on traditional Chinese herbal medicines and small molecular compounds, which comprises the following components in parts by weight: 20-300 g of fructus cnidii, 8-120 g of honeysuckle, 5-75 g of radix sophorae flavescentis, 20-300 g of golden cypress, 25-300 g of rheum officinale, 1-15 g of aloe, 0.1-1.5 g of menthol and 0.1-1.5 g of chlorhexidine acetate. The main effective components of the bacteriostatic agent are plant components, the pH value of the preparation is about 4, the preparation is basically consistent with the female vaginal environment, and the problem of breaking the female private environment is solved; the honeysuckle, the radix sophorae flavescentis, the fructus cnidii, the golden cypress and the rhubarb in the formula are used together, so that the heat-clearing and detoxifying effects are excellent, the menthol is added to achieve the cooling and itching-relieving effects, the aloe is added to increase the lubrication degree of the vagina, and the effects of clearing heat, detoxifying, diminishing inflammation and relieving itching are achieved to the utmost extent.

Description

Bacteriostatic agent based on traditional Chinese herbal medicines and small molecular compounds, and preparation method and application thereof

Technical Field

The invention relates to a bacteriostatic agent, in particular to a bacteriostatic agent based on traditional Chinese herbal medicines and small molecular compounds, and a preparation method and application thereof.

Background

The female private parts are important organs for metabolic secretion, the vaginal orifice is a portal of two systems of reproduction and urinary, and the female genital tract is easy to be infected with various germs and cause diseases due to the physiological weakness of the female genital tract. Due to pruritus vulvae, leucorrhea with reddish discharge, condyloma, erosion and the like caused by vaginitis, cervicitis, pelvic inflammation and the like caused by pathogenic bacteria infection, the female suffers from insanity, if the female is prevented and treated in time, not only the physical and mental health and the life quality of the female are affected, but also infertility can be caused.

Currently, there are two main types of female personal care products on the market: medicinal lotion with therapeutic function and personal care product with cleaning and sterilizing functions. The former can treat gynecological diseases, but almost does not contain other components except Chinese herbal medicine extract such as surfactant, and only contains medicinal components, and has no cleaning function; although the latter can clean and sterilize, the latter is mainly prepared from a surfactant with a cleaning effect and a bactericide with a sterilizing effect, and the strong cleaning and sterilizing capability can destroy the self-cleaning effect of the body, reduce the immunity of the body, stimulate the skin, destroy the self-acidic environment of the body, break the pH value range for maintaining the health of the private parts of women, cause dysbacteriosis and cause gynecological diseases.

Therefore, the development of a nursing product which is mild, has no stimulation, inhibits and kills harmful bacteria and does not damage the female private environment has important significance.

Disclosure of Invention

The invention aims to solve the technical problems in the prior art. Therefore, the bacteriostatic agent provided by the invention is mild, non-irritant, bacteriostatic and capable of not breaking the private environment of women.

The technical scheme adopted by the invention for solving the technical problems is as follows:

the bacteriostatic agent comprises the following components in parts by weight:

20-300 g of fructus cnidii, 8-120 g of honeysuckle, 5-75 g of radix sophorae flavescentis, 20-300 g of golden cypress, 25-300 g of rheum officinale, 1-15 g of aloe, 0.1-1.5 g of menthol and 0.1-1.5 g of chlorhexidine acetate. .

In a preferred embodiment of the bacteriostatic agent based on traditional Chinese herbal medicines and small molecular compounds provided by the invention, the bacteriostatic agent comprises the following components in parts by weight:

200g of fructus cnidii, 80g of honeysuckle, 50g of radix sophorae flavescentis, 200g of golden cypress, 200g of rheum officinale, 10g of aloe, 1g of menthol and 1g of chlorhexidine acetate.

In a preferred embodiment of the bacteriostatic agent based on traditional Chinese herbal medicines and small molecule compounds provided by the invention, the bacteriostatic agent is a mixture, a gel or a paste.

The invention also provides a preparation method of the bacteriostatic agent, which comprises the following steps:

step one, preparing a chlorhexidine acetate menthol solution: respectively taking 0.1-1.5 g of menthol and 0.1-1.5 g of chlorhexidine acetate into a container, respectively adding 20-100 ml of ethanol to dissolve the menthol and the menthol, and then adding 20-100 ml of glycerol to mix the chlorhexidine acetate solution and the menthol solution for later use;

step two, decocting: decocting fructus Cnidii, flos Lonicerae, radix Sophorae Flavescentis, cortex Phellodendri, radix et rhizoma Rhei, and Aloe in water twice, and filtering to obtain filtrate;

step three, concentration: concentrating the decocted filtrate to 1500 ml;

step four, adjusting the pH value: adding 1-5 g of citric acid into the concentrated solution, adjusting the pH value to 4, cooling, and performing centrifugal filtration to obtain a filtrate;

step five, impurity removal: adding 95% ethanol into the filtrate obtained in the fourth step, standing and filtering to obtain filtrate;

step six, removing alcohol: recovering ethanol in the filtrate in the fifth step by using an ethanol extraction and recovery device until no alcohol smell exists, and performing centrifugal filtration to obtain filtrate;

and step seven, adding the chlorhexidine acetate menthol solution prepared in the step one into the filtrate obtained in the step six, uniformly stirring, adding purified water to 2000ml, uniformly stirring, and filtering to obtain the bacteriostatic agent stock solution.

In a preferred embodiment of the preparation method of the bacteriostatic agent provided by the present invention, in the second step, the decoction includes the following steps:

step two, first decoction: adding 6 times of water, decocting for 1.0h, and filtering to obtain filtrate;

step two, second decoction: adding 4 times of water into the filter residue, decocting for 0.5h, and filtering to obtain filtrate;

and step two, combining the filtrates obtained in the first decoction and the second decoction.

The invention also provides application of the bacteriostatic agent in preparation of a female private part care product.

Compared with the prior art, the bacteriostatic agent based on traditional Chinese herbal medicines and small molecular compounds provided by the invention has the beneficial effects that:

the main effective components of the bacteriostatic agent are plant components, the pH value of the preparation is about 4, the preparation is basically consistent with the female vaginal environment, and the problem of breaking the female private environment is solved; the honeysuckle, the radix sophorae flavescentis, the fructus cnidii, the golden cypress and the rhubarb in the formula are used together, so that the heat-clearing and detoxifying effects are excellent, the menthol is added to achieve the cooling and itching-relieving effects, the aloe is added to increase the lubrication degree of the vagina, and the effects of clearing heat, detoxifying, diminishing inflammation and relieving itching are achieved to the utmost extent.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example one

The embodiment provides a bacteriostatic agent based on traditional Chinese herbal medicines and small molecular compounds, which comprises the following components in parts by weight:

20g of common cnidium fruit, 8g of honeysuckle, 5g of lightyellow sophora root, 20g of amur corktree bark, 25g of rhubarb, 1g of aloe, 0.1g of menthol and 0.1g of chlorhexidine acetate.

Preferably, the bacteriostatic agent may be prepared as a mixture or a gel or a paste.

The preparation method of the bacteriostatic agent comprises the following steps:

step one, preparing a chlorhexidine acetate menthol solution: respectively putting 0.1g of menthol and 0.1g of chlorhexidine acetate into a container, respectively adding 20ml of ethanol for dissolving, then adding 20ml of glycerin for mixing the chlorhexidine acetate solution and the menthol solution for later use;

step two, decocting: decocting fructus Cnidii, flos Lonicerae, radix Sophorae Flavescentis, cortex Phellodendri, radix et rhizoma Rhei, and Aloe in water twice, and filtering to obtain filtrate; the specific decoction comprises the following steps:

step two, first decoction: adding 6 times of water, decocting for 1.0h, and filtering to obtain filtrate;

step two, second decoction: adding 4 times of water into the filter residue, decocting for 0.5h, and filtering to obtain filtrate;

step two, combining the filtrates of the first decoction and the second decoction;

step three, concentration: concentrating the decocted filtrate to 1500 ml;

step four, adjusting the pH value: adding 1-5 g of citric acid into the concentrated solution, adjusting the pH value to 4, cooling, and performing centrifugal filtration to obtain a filtrate;

step five, impurity removal: adding 95% ethanol into the filtrate obtained in the fourth step, standing and filtering to obtain filtrate;

step six, removing alcohol: recovering ethanol in the filtrate in the fifth step by using an ethanol extraction and recovery device until no alcohol smell exists, and performing centrifugal filtration to obtain filtrate;

and step seven, adding the chlorhexidine acetate menthol solution prepared in the step one into the filtrate obtained in the step six, uniformly stirring, adding purified water to 2000ml, uniformly stirring, and filtering to obtain the bacteriostatic agent stock solution.

Example two

The embodiment provides a bacteriostatic agent based on traditional Chinese herbal medicines and small molecular compounds, which comprises the following components in parts by weight:

200g of fructus cnidii, 80g of honeysuckle, 50g of radix sophorae flavescentis, 200g of golden cypress, 200g of rheum officinale, 10g of aloe, 1g of menthol and 1g of chlorhexidine acetate.

Preferably, the bacteriostatic agent may be prepared as a neutralizing agent or a gel or a paste.

The preparation method of the bacteriostatic agent comprises the following steps:

step one, preparing a chlorhexidine acetate menthol solution: respectively putting 1g of menthol and 1g of chlorhexidine acetate into a container, respectively adding 50ml of ethanol for dissolving, then adding 50ml of glycerol to mix the chlorhexidine acetate solution and the menthol solution for later use;

step two, decocting: decocting fructus Cnidii, flos Lonicerae, radix Sophorae Flavescentis, cortex Phellodendri, radix et rhizoma Rhei, and Aloe in water twice, and filtering to obtain filtrate; the specific decoction comprises the following steps:

step two, first decoction: adding 6 times of water, decocting for 1.0h, and filtering to obtain filtrate;

step two, second decoction: adding 4 times of water into the filter residue, decocting for 0.5h, and filtering to obtain filtrate;

step two, combining the filtrates of the first decoction and the second decoction;

step three, concentration: concentrating the decocted filtrate to 1500 ml;

step four, adjusting the pH value: adding 1-5 g of citric acid into the concentrated solution, adjusting the pH value to 4, cooling, and performing centrifugal filtration to obtain a filtrate;

step five, impurity removal: adding 95% ethanol into the filtrate obtained in the fourth step, standing and filtering to obtain filtrate;

step six, removing alcohol: recovering ethanol in the filtrate in the fifth step by using an ethanol extraction and recovery device until no alcohol smell exists, and performing centrifugal filtration to obtain filtrate;

and step seven, adding the chlorhexidine acetate menthol solution prepared in the step one into the filtrate obtained in the step six, uniformly stirring, adding purified water to 2000ml, uniformly stirring, and filtering to obtain the bacteriostatic agent stock solution.

EXAMPLE III

The embodiment provides a bacteriostatic agent based on traditional Chinese herbal medicines and small molecular compounds, which comprises the following components in parts by weight:

300g of common cnidium fruit, 120g of honeysuckle flower, 75g of lightyellow sophora root, 300g of amur corktree bark, 300g of rhubarb, 15g of aloe, 1.5g of menthol and 1.5g of chlorhexidine acetate.

Preferably, the bacteriostatic agent may be prepared as a neutralizing agent or a gel or a paste.

The preparation method of the bacteriostatic agent comprises the following steps:

step one, preparing a chlorhexidine acetate menthol solution: respectively taking 1.5g of menthol and 1.5g of chlorhexidine acetate into a container, respectively adding 100ml of ethanol for dissolving, then adding 100ml of glycerol to mix the chlorhexidine acetate solution and the menthol solution for later use;

step two, decocting: decocting fructus Cnidii, flos Lonicerae, radix Sophorae Flavescentis, cortex Phellodendri, radix et rhizoma Rhei, and Aloe in water twice, and filtering to obtain filtrate; the specific decoction comprises the following steps:

step two, first decoction: adding 6 times of water, decocting for 1.0h, and filtering to obtain filtrate;

step two, second decoction: adding 4 times of water into the filter residue, decocting for 0.5h, and filtering to obtain filtrate;

step two, combining the filtrates of the first decoction and the second decoction;

step three, concentration: concentrating the decocted filtrate to 1500 ml;

step four, adjusting the pH value: adding 1-5 g of citric acid into the concentrated solution, adjusting the pH value to 4, cooling, and performing centrifugal filtration to obtain a filtrate;

step five, impurity removal: adding 95% ethanol into the filtrate obtained in the fourth step, standing and filtering to obtain filtrate;

step six, removing alcohol: recovering ethanol in the filtrate in the fifth step by using an ethanol extraction and recovery device until no alcohol smell exists, and performing centrifugal filtration to obtain filtrate;

and step seven, adding the chlorhexidine acetate menthol solution prepared in the step one into the filtrate obtained in the step six, uniformly stirring, adding purified water to 2000ml, uniformly stirring, and filtering to obtain the bacteriostatic agent stock solution.

The gel or paste in the above embodiments can be further prepared from a bacteriostatic stock solution.

In the formulation of the above example:

fructus Cnidii is dried mature fruit of cnidium monnieri (L.) DC of Umbelliferae. Collected in summer and autumn when the fruits are ripe, removed of impurities and dried in the sun. The functional indications are as follows: dry dampness, dispel wind, kill parasites. Can be used for treating lumbago due to cold womb, leukorrhagia due to cold-dampness, and arthralgia due to dampness; it can be used for treating vulval eczema, pudendal pruritus, and trichomonal vaginitis.

Flos Lonicerae is the dried bud or flower with initial bloom of Lonicera Japonica of Lonicera of Caprifoliaceae and plants of the same genus. The honeysuckle flower can disperse wind-heat and clear away blood toxin, and has obvious effect on various heat diseases such as fever, eruption, freckles and the like.

Radix Sophorae Flavescentis is dried root of radix Sophorae Flavescentis of Leguminosae. Has effects of clearing heat, eliminating dampness, killing parasite, and promoting urination. Mainly used for treating damp-heat diarrhea and dysentery, hematochezia, jaundice, damp-heat leucorrhea, vulval swelling and pruritus vulvae, eczema and eczema, skin pruritus, scabies and tinea, damp-heat dysuria.

Cortex Phellodendri is dried bark of phellodendron amurense belonging to genus phellodendron of family Rutaceae. Has the effects of clearing heat, eliminating dampness, purging pathogenic fire and removing toxic substances. Can be used for treating leukorrhagia due to damp-heat, stranguria due to heat, dysentery due to damp-heat, jaundice, tinea pedis due to damp-heat, flaccidity syndrome, hectic fever due to yin-deficiency, night sweat, spermatorrhea, pyocutaneous disease, toxic swelling, eczema, and pruritus.

Rhubarb, which is a composite name of perennial plants of rhubarb of Polygonaceae, has red stem, fragrant smell, bitter and slightly astringent taste, sticky teeth when being chewed and gritty. Collected in late autumn before stem and leaf withered or germination in spring. Removing fine roots, scraping outer skin, cutting into sections or segments, stringing, and drying or directly drying. The Chinese medicine rhubarb has the functions of eliminating stagnation, clearing away damp-heat, purging fire, cooling blood, eliminating blood stasis, detoxicating, etc.

Aloe is dried concentrated juice of Aloe barbadensis Miller and Aloe ferox Miller belonging to Aloe of Liliaceae. Has the effects of relaxing bowels with purgative, clearing liver heat and killing parasites.

Mentholum is extracted from leaf and stem of herba Menthae, is Chinese medicinal extract, white crystal, and is the main ingredient of herba Menthae and Mentha piperita essential oil. It can be applied to skin or mucosa, and has refreshing and antipruritic effects.

Chlorhexidine acetate: the application is an external high-efficiency safe antibacterial disinfectant, and can kill staphylococcus aureus, escherichia coli and candida albicans. The substance with very low concentration (five parts per million) can kill the bacteria in the extracting solution, so that the extracting solution can better protect the vaginal environment of women.

Stability testing of the invention:

under the constant temperature condition of 20 +/-1 ℃, the test is repeated for 3 times, after the bacteriostatic solution sample stock solution is placed in a constant temperature and humidity box with the temperature of 37 ℃ and the relative humidity of 80% for 90 days, the sample has no color change, no layering and no precipitation, the average bacteriostatic rates of the sample on candida albicans in 2min, 5min, 10min and 20min are respectively 95.25%, 98.14, 100% and 100%, and the test results are as follows:

the test is repeated for 3 times under the constant temperature condition of 20 +/-1 ℃, and the average bacteriostasis rate of the bacteriostasis liquid sample stock solution on staphylococcus aureus is 100% after 2min, 5min, 10min and 20 min. The test results are given in the following table:

the test is repeated for 3 times under the constant temperature condition of 20 +/-1 ℃, and the average bacteriostasis rate of the bacteriostasis liquid sample stock solution on the escherichia coli is 100% after 2min, 5min, 10min and 20 min. The test results are given in the following table:

the above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (6)

1. A bacteriostatic agent based on traditional Chinese herbal medicines and small molecular compounds is characterized in that: the bacteriostatic agent comprises the following components in parts by weight:

20-300 g of fructus cnidii, 8-120 g of honeysuckle, 5-75 g of radix sophorae flavescentis, 20-300 g of golden cypress, 25-300 g of rheum officinale, 1-15 g of aloe, 0.1-1.5 g of menthol and 0.1-1.5 g of chlorhexidine acetate.

2. The bacteriostatic agent based on traditional Chinese herbal medicines and small molecule compounds according to claim 1, characterized in that: the bacteriostatic agent comprises the following components in parts by weight:

200g of fructus cnidii, 80g of honeysuckle, 50g of radix sophorae flavescentis, 200g of golden cypress, 200g of rheum officinale, 10g of aloe, 1g of menthol and 1g of chlorhexidine acetate.

3. The bacteriostatic agent based on traditional Chinese herbal medicines and small molecule compounds according to claim 1, characterized in that: the bacteriostatic agent is a mixture, gel or paste.

4. A method for preparing a bacteriostatic agent according to any one of claims 1 to 3, which is characterized by comprising the following steps: the method comprises the following steps:

step one, preparing a chlorhexidine acetate menthol solution: respectively taking 0.1-1.5 g of menthol and 0.1-1.5 g of chlorhexidine acetate into a container, respectively adding 20-100 ml of ethanol to dissolve the menthol and the menthol, and then adding 20-100 ml of glycerol to mix the chlorhexidine acetate solution and the menthol solution for later use;

step two, decocting: decocting fructus Cnidii, flos Lonicerae, radix Sophorae Flavescentis, cortex Phellodendri, radix et rhizoma Rhei, and Aloe in water twice, and filtering to obtain filtrate;

step three, concentration: concentrating the decocted filtrate to 1500 ml;

step four, adjusting the pH value: adding 1-5 g of citric acid into the concentrated solution, adjusting the pH value to 4, cooling, and performing centrifugal filtration to obtain a filtrate;

step five, impurity removal: adding 95% ethanol into the filtrate obtained in the fourth step, standing and filtering to obtain filtrate;

step six, removing alcohol: recovering ethanol in the filtrate in the fifth step by using an ethanol extraction and recovery device until no alcohol smell exists, and performing centrifugal filtration to obtain filtrate;

and step seven, adding the chlorhexidine acetate menthol solution prepared in the step one into the filtrate obtained in the step six, uniformly stirring, adding purified water to 2000ml, uniformly stirring, and filtering to obtain the bacteriostatic agent stock solution.

5. The method of preparing a bacteriostatic agent according to claim 4, wherein: in the second step, the decoction comprises the following steps:

step two, first decoction: adding 6 times of water, decocting for 1.0h, and filtering to obtain filtrate;

step two, second decoction: adding 4 times of water into the filter residue, decocting for 0.5h, and filtering to obtain filtrate;

and step two, combining the filtrates obtained in the first decoction and the second decoction.

6. Use of a bacteriostatic agent according to any one of claims 1 to 3 in the preparation of a female personal care product.