KR102061302B1 - Feminine cleanser having antimicrobial and deodorizing function - Google Patents

Abstract

The present invention relates to an antibacterial and deodorizing functional feminine detergent composition which exhibits antibacterial and deodorizing effect by inhibiting vaginal bacterium and helping to grow lactic acid bacteria, characterized by including a citric acid buffer including citric acid and citrate.

Description

Feminine cleanser having antimicrobial and deodorizing function

The present invention relates to an antibacterial and deodorant functional feminine detergent composition, and more particularly, to an antibacterial and deodorant functional feminine detergent composition which exerts an antibacterial and deodorant effect by inhibiting vaginal bacterium and helping to grow lactic acid bacteria.

Unlike males, females have complex reproductive functions that are controlled by various hormones, as well as anatomically fragile structures, so female genitalia are always susceptible to bacterial invasion from the outside. In sexually mature women, inflammation in the vagina can lead to sexual dysphoria, pain, etc., so it is good to prevent genital damage from invading bacteria in advance. Vaginitis is the most common gynecological disease among Korean women. Vaginitis is not aggravated in severe condition or complications in the Middle East despite the obvious symptoms, but it is difficult to recover from treatment and recurs easily. As a result, women, especially middle-aged women, it is desirable to always keep the vagina clean and not to be exposed to bacterial infection from the outside.

Vaginitis differs according to the age group of women because vaginal tissues vary with age. For example, the epithelium of the vagina of mature young women is thick and elastic, with significantly fewer gonococcal diseases. However, in middle-aged women, atrophy of the vagina is apt to be affected by various germs including gonococcus.

In addition, the vaginitis can be infected by swimming pools or baths, most of which are caused by candida mildew, and even women without biblical experience. And middle-aged women in menopause are not only concerned with this problem, but also with urinary incontinence, causing a loss of confidence in their sex lives.

On the other hand, lactobacillus among normal bacteria living in the vagina plays an important role in maintaining the acidity of vaginal secretions, and this acidity is due to the organic acid generated by the breakdown of glycogen, a glycogen in vaginal epithelial cells. Therefore, the vagina maintains a constant pH, pH 4.0 to 4.5, and the vagina becomes more easily infected from foreign bacteria when it is deviated from the normal pH by any physiological change.

Therefore, conventional women's vaginal cleaners contain chlorohexidine gluconate, benzalkonium chloride, or trichlorocarbanilide for the purpose of sterilizing lactic acid and bacteria in the vagina to maintain acidity of the vagina. And the like have been used.

However, such a cleansing agent is a physicochemical agent, so the cleaning effect is deteriorated when used for a long time, various bacteria are removed instead of fungi, and odors and unpleasant odor removal effects are limited.

Since the use of vaginal cleaners is not satisfactory in preventing or treating vaginitis, it is necessary to develop a vaginal cleaner that can effectively improve the quality of women's vaginal health.

Republic of Korea Patent Application Laid-Open Publication No. 2016-0138824 No. (title of the invention: vaginosis therapeutic composition) is "The invention as stearyl Ido bakteo Denny pecan tree's (Steroidobacter denitrificans) YH1 (KCTC18345P) and Lactobacillus Cri Spa tooth (Lactobacillus crispatus ) relates to a composition for treating vaginal disease comprising the YH2 (KCTC18346P) strain, thereby providing a lactic acid bacterium-containing vaginal therapeutic composition having excellent therapeutic effect against bacterial vaginosis in women. "

It is an object of the present invention to provide an antibacterial and deodorizing functional feminine detergent composition which exhibits antibacterial and deodorizing effect by inhibiting vaginal bacterium by using citric acid buffer including a mixture of citric acid and citrate, and helping to grow lactic acid bacteria. . The addition of gluconolactone to produce lactic acid as an energy source and organic acid is beneficial to lactic acid bacteria to have a selective antibacterial activity.

Antibacterial and deodorant functional female detergent composition according to the present invention is characterized in that it comprises a citric acid buffer comprising citric acid and citrate.

The citrate can be sodium citrate, magnesium citrate or potassium citrate, and the sodium citrate can be trisodium citrate, disodium citrate, or monosodium dihydrogen citrate.

Citric acid buffer may be one having a pH within the range of 3.0 to 5.0.

Citric acid buffer may be one having a concentration within the range of 0.001 to 1M.

The composition may further comprise gluconolactone.

The composition may comprise gluconolactone in an amount within the range of 0.001 to 10% by weight.

The composition may have a formulation selected from the group consisting of powders, tablets, solutions, gels, sprays, and coatings.

According to the present invention, the effect of providing an antibacterial and deodorizing functional feminine detergent composition exhibiting an antibacterial and deodorizing effect by inhibiting vaginal bacterium by using a citric acid buffer containing a mixture of citric acid and citrate and helping the growth of lactic acid bacteria is effective. have.

Figure 1 is a graph showing the results of measuring the pH stability of the antibacterial and deodorant functional female detergent composition according to one embodiment of the present invention.
2 and 3 are graphs and photographs showing the results of measuring the antimicrobial properties (target G. vaginalis ) of the antibacterial and deodorant functional women's detergent composition according to one embodiment of the present invention.
4 and 5 are graphs and photographs showing the results of measuring the antimicrobial properties (target L. acidophilus ) of the antibacterial and deodorant functional women's detergent composition according to one embodiment of the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Antibacterial and deodorant functional female detergent composition according to the present invention is characterized in that it comprises a citric acid buffer comprising citric acid and citrate.

Citric acid (citric acid) is one of the polybasic carboxylic acid having a hydroxyl group (-OH) is also called citric acid (枸 祁 酸). Formula C ₆ H ₈ O ₇ . Many seeds and juices contain free acid. Citrus is the name of citron in citron, and is derived from citrus, lemon, and unripe citrus fruits. The citric of the English name citric acid is derived from citrus, the Greek word citrus. Soluble in water and ethanol. When microorganisms are cultured using sugar as a substrate, citric acid accumulates in the culture solution, which is called citric acid fermentation. Several culture methods have been studied, and now 90% of the world's total citric acid is produced by this fermentation method. As a microorganism causing citric acid fermentation, black mold is usually used. Citric acid can be obtained by fermenting at about 30 ° C. for 7 to 10 days in an acid (pH 2 to 3). In addition, citric acid plays an important role in metabolism of higher animals. It is also known to promote calcium absorption in the body. It is used as an analytical reagent in addition to adding to juices and soft drinks, or adding sourness to medicines and diuretic beverages. In addition, calcium ions are required for blood coagulation, and citric acid is used as a blood coagulation inhibitor because it traps calcium ions.

Citric acid is also used for food. Citric acid for food includes crystals (monohydrate) and anhydrides, and each is called crystalline citric acid and citric anhydride. Used as an acidulant. Citric acid as a raw material is harmless to the human body, and a product sold as a powder may be used.

Citric acid (crystals): Weathers slowly in a dry atmosphere and slowly dehydrates in moist air. When hot, it melts and carbonizes, generating a vapor with an irritating odor. It melt | dissolves 209g at 25 degreeC with respect to 100 ml of water, and melt | dissolves also in ethanol and ether. The pH of the aqueous solution is 2.31 (1% liquid), 1.83 (5% liquid), 1.63 (10% liquid), 0.48 (70% liquid). Specific gravity is 1.542 and melting point is 135-152 degreeC. It is used as a synergist of acidic seasonings and cooking oils such as soft drinks, mixed liquors, candy, jelly, jams, frozen desserts, and canned foods.

Citric acid (anhydrous): Anhydride is obtained by making the crystal precipitation temperature of citric acid into 37 degreeC or more. It dissolves 59.2 g at 20 ° C, 68.6g at 40 ° C, 73.5g at 60 ° C, 78.8g at 80 ° C, and 84.0g at 100 ° C, and melts up to 62% in anhydrous ethanol with respect to 100ml of water. The pH of the aqueous solution is 2.25 (1% liquid), 1.87 (5% liquid), 1.68 (10%), 0.39 (70%). Specific gravity is 1.67, Melting point is 153 ° C. Powder juice, powdered foam juice, powder sherbet, powder ketchup, chewing gum, etc. are used for foods that do not like moisture or acidulants of powdered food. Since there is no number of crystals, the acidity is stronger than citric acid (crystal) and at least about 10% when used. There is no usage standard. It is a colorless and transparent crystal. Kernel or lump, or white crystalline powder or powder, odorless, strong acidity.

Citrate salts mean salts of citric acid. Such salts may be formed with sodium, magnesium or potassium. Sodium citrate may be present as trisodium citrate, disodium citrate, or monosodium dihydrogen citrate. The citrate can be sodium citrate, magnesium citrate or potassium citrate, and the sodium citrate can be trisodium citrate, disodium citrate, or monosodium dihydrogen citrate. Citrate as a raw material is harmless to the human body, and a product sold as a powder may be used.

Citric acid buffer according to the present invention may be one having a pH within the range of 3.0 to 5.0. Within this range, citric acid buffers in particular promote the growth of lactic acid bacteria ( L. acidophilus, KACC12419), dominant species, thereby inhibiting the growth of vaginitis ( G. vaginalis , KCTC5096) to provide effects such as vaginitis prevention. When the pH of citric acid buffer is less than 3.0, it may be irritating to human body and may be unpleasant to use, and even the growth of lactic acid bacteria may be even suppressed. Relatively, the effect of inhibiting the growth of vaginal infections may be insufficient.

Citric acid buffer may be one having a concentration within the range of 0.001 to 1M. If the concentration of citric acid is less than 0.001M, there may be a problem in the buffer effect to suppress the pH change, on the contrary, if it exceeds 1M, high osmotic pressure may be generated to worsen the vaginal environment.

The composition may further comprise gluconolactone. Gluconolactone is a heterocyclic compound containing -C (= 0) -0-. Generally, it refers to a compound cyclized by an ester bond formed between a carboxyl group of a hydroxy acid and a hydroxy group. The ring is released relatively easily. δ-gluconolactone is unsaturated in an aqueous solution to become acidic. It is a food additive to swell the product during the manufacture of bread and confectionery to improve the taste and soften the digestion. It is used as a coagulant, a slow-release powder of a synthetic expanding agent, an acidulant, an acidity regulator, an antioxidant, and the like. White crystalline or crystalline powder, odorless or slightly odorous, initially sweet but slightly acidic. Synthetic expanding agent, well soluble in water and slightly soluble in ethanol, but insoluble in ether. The aqueous solution is slowly hydrolyzed to equilibrate Gluconic acid with δ-lactone and γ-lactone, and the higher the temperature or pH, the faster hydrolysis occurs. After about 2 hours at room temperature of 25 ℃ completely hydrolyzed to a solution of 55-60% gluconic acid, 40-50% lactone. Although glucono-δ-lactone is not an acid, when dissolved in water, it is hydrolyzed to show acidity. Therefore, it is recommended to be used as an acidity regulator for swelling agent. In addition, hydrolysis occurs slowly. It also has antioxidant properties because it forms complex salts with metals, although it is not an acid, the aqueous solution shows acidity by heating. Gluconic acid is a sour crystal and has a melting point of 130 to 132 ° C and a molecular weight of 196.2. Forms D, L and racemates are known, with D-gluconic acid being the most important. Form D is generally produced by oxidation of D-glucose or oxidation of a substance consisting of D-glucose, and also by oxidation, electrolytic oxidation and fermentation of bromine water in an aqueous D-glucose solution. It is present in vivo in the form of 6-phosphate ester as an intermediate of sugar metabolism. Since it is an intermediate of sugar metabolism, it can be a food for bacteria, and because it is acidic, it promotes the growth of lactic acid bacteria ( L. acidophilus, KACC12419) together with the citric acid buffer described above, and thereby dominates the vaginal bacterium ( G. vaginalis , KCTC5096). ) To inhibit the growth of the yeast infection and provide effects. Gluconolactone as a raw material is harmless to the human body, and a product sold as a powder may be used.

The composition according to the invention may comprise gluconolactone in an amount within the range of 0.001 to 10% by weight. If the concentration of gluconolactone is less than 0.001% by weight based on the total weight of the composition, there may be a problem that is not sufficient to exert selective antimicrobial activity, on the contrary, when it exceeds 10% by weight, it is not dissolved and is very sticky. There may be problems such as causing dehydration of the vagina at high concentrations.

The composition may have a formulation selected from the group consisting of powders, tablets, solutions, gels, sprays, and coatings. The formulations may further comprise components used in conventional detergents, such as human detergent compositions. Examples include metal ion sequestrants, preservatives, perfumes, colorants, surfactants, diluents, alcohols, humectants and conditioning agents. Examples of surfactants include sodium laureth sulfate, cocamidopropyl betaine and alkyl glucosides.

Hereinafter, preferred embodiments and comparative examples of the present invention will be described.

The following examples are intended to illustrate the invention and should not be understood as limiting the scope of the invention.

Example 1 Preparation of a Single Material and Mixture Solution

Prepared by dissolving citric acid, sodium citrate, lactic acid, gluconolactone, glucose, salt, and a mixture of glucose and salt (glucose: salt in weight ratio = 2: 1; 1: 1; 1: 2) in purified water In addition, the nine solutions and the glycerol solution was measured in the antimicrobial activity in Experimental Example 1.

Experimental Example 1 Measurement of Antimicrobial Activity of a Single Substance and Mixture

The antimicrobial activity of the solutions prepared in Example 1 was measured.

Strains and Media

Vaginitis ( G. vaginalis ; KCTC5096) was lyophilized at the Korea Research Institute of Bioscience and Biotechnology and was inoculated into modified BHI medium and used after passage in 37 ° C. and 10% CO ₂ incubator. Modified BHI medium is a medium containing 3.7% BHI powder and 10% horse serum.

Lactobacillus ( L. acidophilus ; KACC12419) was freely distributed at the National Academy of Agricultural Sciences, inoculated in MRS medium, and subcultured in a 37 ° C. incubator.

After diluting the solutions of the single substance and the mixture prepared in Example 1 by concentration (0.1 vol% to 40 vol%) and adding a strain (final concentration 10 ⁷ cfu / ml) to incubate for 24 hours in liquid culture conditions, 100 μl of agar was added to the solid medium to confirm the cracking to obtain the MBC value. At this time, the MBC value was reduced to 99.9% of the initial bacterial count, and the results are shown in Table 1 below.

Since lactic acid bacteria inhibit the growth of harmful bacteria by controlling the acidity in the vagina, the ratio of MBC values of lactic acid bacteria and vaginitis-inducing bacteria was calculated.

G. vaginalis L. acidophilus MBC _{L.acidophilus} / MBC _G.vaginalis Citric acid 0.1% 0.5% 5 Sodium citrate One% One% One Lactic acid 0.2% One% 5 Gluconolactone 0.2% One% 5 Glycerol 40% 40% One glucose 40% 40% One Salt 5% 5% One Glucose: Salt
(2: 1) 20% 20% One Glucose: Salt
(1: 1) 10% 10% One Glucose: Salt
(1: 2) 10% 10% One

As it is shown in Table _{1, MBC L .acidophilus / MBC G} . _vaginalis Citric acid, lactic acid, and gluconolactone with a ratio of 5 were selected, and sodium citrate was also selected for pH control.

Example 2 Preparation of Female Cleanser Composition

After the content of citric acid and sodium citrate was prepared to be 80 mM in an aqueous solution, Examples 2-1 to 2-4 were prepared by adding lactic acid and gluconolactone as shown in Table 2 below. Gluconolactone proceeds with hydrolysis in aqueous solution to reduce the pH of the solution.

Citric acid Sodium citrate Lactic acid Gluconolactone Purified water total Example 2-1 0.421 1.708 0.3 0.05 97.521 100 Example 2-2 0.421 1.708 0.3 0.2 97.371 100 Example 2-3 0.421 1.708 0.3 0.5 97.071 100 Example 2-4 0.421 1.708 0.3 1.0 96.571 100

Experimental Example 2 pH Measurement

Immediately after preparing the compositions according to the above Examples, the pH values were measured and compared at the time points after 24 hours, 72 hours, and 7 days, and the results are shown in Table 3 below.

Immediately 24h 72h 7 days Example 2-1 4.21 4.09 3.99 3.99 Example 2-2 4.17 4.01 3.93 3.93 Example 2-3 4.01 3.86 3.82 3.82 Example 2-4 3.85 3.66 3.56 3.56

As shown in Table 3, Example 2-3 appeared to maintain a pH value of 3.82 even after 7 days, which was used in the next experiment.

Experimental Example 3 pH Stability at Alkali Solution Addition

Example 2-3 pH stability was measured by adding 1 ml of 1N NaOH as an alkaline solution to 100 ml of the solution. For comparison, a citric acid solution having a pH of 3.80 was prepared, and pH stability was measured by adding NaOH in the same manner, and the results are shown graphically in FIG. 1.

As shown in FIG. 1, the solution containing citric acid alone increased the pH to 11.32 when 1 ml of 1N NaOH was added at pH 3.8, whereas the Example 2-3 solution maintained weak acidity until 13 ml was added. Could.

Experimental Example 4 Growth Inhibition Effects on Vaginitis-Inducing Bacteria and Lactic Acid Bacteria

Strains and Media

Vaginitis ( G. vaginalis ; KCTC5096) was lyophilized at the Korea Research Institute of Bioscience and Biotechnology and was inoculated into modified BHI medium and used after passage in 37 ° C. and 10% CO ₂ incubator. Modified BHI medium is a medium containing 3.7% BHI powder and 10% horse serum.

Lactobacillus ( L. acidophilus ; KACC12419) was freely distributed at the National Academy of Agricultural Sciences, inoculated in MRS medium, and subcultured in a 37 ° C. incubator.

Example 2-3 was diluted by distilled water by concentration (final concentration 0.1% to 50% by volume), and G. vaginalis and L. acidophilus strains (final concentration 10 ⁷ cfu / ml) were respectively added to the liquid culture conditions. Incubated for 72 hours. The absorbance of each time was measured at 600 nm, and Example 2-3 compared the effect on the growth of the two strains. 100 μl of each agar was added to the solid medium to which the agar was added to confirm the cracking to obtain MBC values.

As a result, the antimicrobial activity of Example 2-3 was 10% in G. vaginalis , 20% in L. acidophilus , as shown in Table 4, it was confirmed that the growth of harmful bacteria more effectively than the growth of beneficial bacteria. .

G. vaginalis L. acidophilus MBC value 10% 20%

In addition, as shown in Figures 2 to 5, Example 2-3 effectively inhibited the growth of G. vaginalis after 4 hours at a concentration of 10%, it was shown to inhibit 99.999% after 16 hours. At the same concentration, the growth of L. acidophilus was not inhibited, and after 72 hours, the absorbance value was similar to that of the control group (the same amount of distilled water was used instead of Example 2-3).

Experimental Example 5 Deodorizing Effect on Ammonia and Trimethylamine

The nonwoven fabric was cut to a size of 5 × 5 cm, then uniformly applied in 1 ml samples, and the nonwoven fabric was dried at 60 ° C. The nonwoven fabric was transferred to a 3 L test vessel, treated with ammonia and trimethylamine at concentrations of 40 ppm and 20 ppm, respectively. The residual concentration was confirmed by connecting the pipes, and the results are shown in Table 5 below.

ammonia Trimethylamine Control (ppm) 40 20 After 5 minutes (ppm) One 2 10 minutes later (ppm) 0 0

As shown in Table 5, ammonia and urine odor-inducing substance trimethylamine was inhibited by 90% or more after 5 minutes, 100% after 10 minutes.

Experimental Example 6 Antiseptic Test

Antiseptic test was performed for Example 2-3 against E. coli , S. aureus , and Candida ( C. albicans ) in the following manner. E. coli , S. aureus and C. albicans were inoculated into the composition at 10 ⁵ cfu / ml, 10 ⁵ cfu / ml, and 10 ³ cfu / ml, respectively, and observed until Day 1, Day 3, and Day 7, The results are shown in Table 6 below.

E. coli , S. aureus and C. albicans showed 99% growth inhibition in 3 days and showed excellent antiseptic ability.

Microorganism Classification Early Day 1 Day 3 Day 7 E. coli 10 ⁵ cfu / ml 30 0 0 S. aureus 10 ⁵ cfu / ml 616 0 0 C. albicans 10 ³ cfu / ml 1350 10 0

Experimental Example 7 Skin Primary Stimulation Test

In order to confirm the skin irritation of the composition of Example 2-3, the primary stimulation test evaluation for the skin irritation was performed.

Subject selection criteria were administered to men and women aged 18-60 years, and those with skin diseases were excluded from the subject.

After disinfecting the test site with alcohol, 200 uL of sample was placed on cotton wool and attached to the same area inside the arm using a gauze and a band-aid. Sterile water was used as a control. The patch was run for 24 hours, and the test site was kept out of water for 24 hours.

As shown in Table 7 below, no adverse effects such as erythema, itching and swelling were found on the skin.

No. Sample name Side effects case 24 hours later Response grade average Grade 1 Grade 2 Grade 3 Grade 4 One Control 0 - - - - 0 2 Example 2-3 0 - - - - 0

Experimental Example 8 Clinical Trial

After using the composition of Example 2-3 to 25 women in their 10s to 40s for 3 months, a survey was conducted on deodorant effect, pruritus improvement, moisturizing effect, and sedative effect, respectively, very satisfied, satisfied, and moderate , Dissatisfied, and very dissatisfied are shown in Table 8 below. As shown in Table 8, the female detergent composition showed a very high satisfaction of 96% in the deodorant effect, it is shown to exhibit an excellent effect in antibacterial, deodorant and moisturizing by showing a high satisfaction of 80% or more in other effects.

Example 2-3 very good Satisfaction usually dissatisfaction very unsatisfied Deodorant effect 24 0 One 0 0 Pruritus improvement 20 2 2 0 One Moisturizing effect 20 One One One 2 Soothing effect 23 One One 0 0

Although the present invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical scope of the present invention, and such modifications and modifications belong to the appended claims.

(Not drawing)

Claims (7)

A citric acid buffer comprising citric acid and sodium citrate, and an antibacterial and deodorizing functional feminine detergent composition comprising a gloconolactone in an amount in the range of 0.001 to 10% by weight and no lactic acid bacteria. The method of claim 1,
The sodium citrate is antibacterial and deodorant functional female detergent composition, characterized in that trisodium citrate, disodium citrate, or monosodium dihydrogen citrate. The method of claim 1,
Antimicrobial and deodorant functional feminine cleaner composition, characterized in that citric acid buffer has a pH within the range of 3.0 to 5.0. The method of claim 3, wherein
Citric acid buffer antibacterial and deodorant functional female detergent composition, characterized in that it has a concentration within the range of 0.001 to 1M. delete delete The method according to any one of claims 1 to 4,
An antimicrobial and deodorizing functional feminine detergent composition, wherein the composition has a formulation selected from the group consisting of powders, tablets, solutions, gels, sprays, and coatings.

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