KR20150107376A - Antibacterials composition comprising Sedum extract composition and food additives and preparation method thereof - Google Patents

Abstract

The present invention provides a manufacturing method of an antimicrobial raw material, regarding an antimicrobial raw material using a Sedum takevimense NAKAI. extract. The antimicrobial raw material comprises: a) a Sedum takevimense NAKAI. extract; and b) at least one species selected from a Yucca gloriosa L. extract, vitamin B1 thiamine dilaurylsulfate, sorbic acid, benzoic acid, and a mixture thereof. In addition, the antimicrobial raw material manufactured with the method composed of a safe food and a food additive, has preservative effects by being added to wet tissue, cosmetics, or household items.

Description

[0001] The present invention relates to an antibacterial raw material containing an extract of an island giraffe, a food additive, and a method for producing the antibacterial raw material,

The present invention relates to an antibacterial raw material containing an island giraffe extract, and more particularly, to an antibacterial raw material comprising a mixture of at least one selected from the group consisting of an island giraffe extract, an yucca extract, a vitamin B1 lauryl sulfate, a sorbic acid and a benzoic acid. will be.

The present invention is characterized in that it is added to daily necessities such as wipes, cosmetics, diapers, menstrual supplies, oral tissues, mouthwashes, shampoos, soaps, shower gels and the like, which are antibacterial raw materials composed of foods and food additives which are harmless to humans, .

In everyday life, microorganisms such as bacteria and fungi are not only very abundant but also soil, atmospheric, and water. Seawater, etc., and it is possible to grow and reproduce at any time if the growth conditions are right. Therefore, microbial damage to human diseases caused by microorganisms, food spoilage, housing, clothing, and industrial products is serious .

Above all, foods, household goods, medicines and cosmetics are necessary to use preservatives in order to prevent microbial spoilage and to preserve the product for a long time. Preservative is a medicine that prevents the decay of a material. It is a preservative to prevent the vegetable organic material from decaying due to the action of microorganisms, and a preservative is added to preserve it for the purpose of conservation. Preservatives that prevent microbial decay are essential for long-term quality of products such as food, medicines and cosmetics.

Generally, in order to remove or suppress the occurrence of microorganisms propagating in diapers, wipes, sanitary articles, cosmetics, detergents, disinfectant products and the like, chloromethylisothiazolinone (CMIT), 3-iodo- 3-lodo-2-propynylbutylcarbamate (IPBC), polyhexamethylene guanidine (PHMG), polyhexamethylene biguanide (PHMB), 2- (2-ethoxy) ethoxyethyl guanidine chloride (PGH), alcohols, benzalkonium chloride And chemical antimicrobial agents are mainly used.

However, these chemical preservatives inhibit the growth of microorganisms and impart stability, but at the same time, they cause stimulation of the user's skin. For example, PHMG, PHMB, and PGH are known to cause harmful effects of guanidine-based chemicals and 3-iodo-2-propynyl butylcarbamate, which are the causative agents of acute lung injury syndrome caused by humectant cleaning agents, It is known that there is a strong toxicity problem.

Preservatives such as parabens, which are commonly used in cosmetics and medicines as a conventional preservative, are also known as skin allergies (Andrea Counti et al., 1997, 37; 35-36) and the possibility of environmental hormones (Edwin et al., Toxicology and Applied Pharmacology, 1998, 153: 12-19) and inducing resistant bacteria.

Due to these problems, studies on natural preservatives, which are excellent in safety and economy of products, have been continued. As a result, natural antimicrobial substances of microbial origin such as extracts of plant and plant origin such as spices, essential oils and herbal medicines and bacteriocins have been reported

Alkaloids, flavonoids, phytoalexins, and antimicrobial peptides are known as natural antimicrobial substances. Antimicrobial activity of organic acids and fatty acids is also known. Most of these are presumed to be the main mechanism by the effect of acid pH and chelate (E1-shenawa, MA et al., J. Food Protec. 1989, 52 (11): 771-778) (Bizri, JN et al. , J. Food Science, 1994, 59 (1), 130-135). However, most of the reported natural antimicrobial substances have not been commercialized because of color, pH drop, narrow antimicrobial spectrum, problem of form, and the like. Hinokitiol, Hinokitiol extract, Megnonol, Extracts, and complex gold extracts.

The present invention relates to an antimicrobial and antioxidative composition comprising an extract of Kirinchiflorus japonica as an active ingredient, and more particularly to an antimicrobial and antioxidant composition which is excellent in safety and economical Is a natural functional material which can be utilized as an antibacterial preservative, a cosmetic, a medicine, a health promotion food, and a feed additive material in a food field.

However, there is a shortage of environmentally friendly antibacterial raw materials that can prevent the deterioration of products requiring strong buoyancy, such as household goods and cosmetics including wet tissues. Furthermore, it is necessary to study the environmentally friendly antimicrobial raw materials which are safer preservatives that are safe food substitutes for CMIT, PHMG and PHG which are harmful to human body and which have secured formulation safety.

Open Patent No. 2012-0139003A (2012.12.27.)

Andrea Counti et al., Contact Dermatitis, 1997, 37; 35-36 Edwin et al., Toxicology and Applied Pharmacology, 1998, 153; 12-19 E1-shenawa, MA et al., J. Food Protec. 1989, 52 (11): 771-778 Bizri, JN et al., J. Food Science, 1994, 59 (1), 130-135

The present invention has been made to solve the above problems of the prior art, and it is an object of the present invention to provide an eco-friendly preservative composition which can reduce the use of a chemical preservative which may be harmful to human body and obtain the same effect as the chemical preservative To a method for producing an antibacterial raw material.

The present invention also relates to a manufacturing method and antibacterial property when an antimicrobial raw material is added to a wipes, cosmetics or daily necessities.

In order to achieve the above object, the present invention provides an antibacterial raw material using an island giraffe extract according to the present invention, wherein the antibacterial raw material is selected from the group consisting of a) a giraffe extract, and b) a yucca extract, vitamin B1 lauryl sulfate, sorbic acid, , And the like.

In one embodiment, the weight ratio of a) and b) may be from 1: 0.4 to 1: 1.5.

In one embodiment, 0.3 to 3 parts by weight of the antibacterial raw material is added to 100 parts by weight of distilled water to be included in the wet tissue liquid.

The antimicrobial raw material may be added to cosmetic raw materials in an amount of 0.5 to 5 parts by weight per 100 parts by weight of the cosmetic formulation composition.

The antimicrobial raw material of the present invention has excellent antimicrobial and antifungal effects against various microorganisms such as Gram-positive bacteria, Gram-negative bacteria, fungi, yeast, and has excellent antibiotic and antifungal effect.

Further, since the antibacterial raw material of the present invention is composed of safe foods and food additives, it has no skin irritation even when added to wet tissues, cosmetics or daily necessities, has a skin safety effect and is harmless to human body.

FIG. 1 is a result of a test of the wettability test of a wet tissue including an antibacterial raw material according to an embodiment of the present invention.
FIG. 2 is a result of the anti-buoyancy test of a cosmetic product containing an antibacterial raw material according to an embodiment of the present invention.

Hereinafter, the manufacturing method of the present invention will be described in detail with reference to the accompanying drawings.

The present invention relates to a method for producing an antibacterial raw material, comprising the steps of: a) mixing an island giraffe extract and b) an extract of yucca, vitamin B1 lauryl sulfate, sorbic acid and benzoic acid, And a weight ratio of 1: 0.4 to 1: 1.5 to prepare an antibacterial raw material.

(Island giraffe extract)

The extract is dried at a temperature of 70 to 90 ° C, extracted with methanol at a rate of 5 to 12 times the weight of the dried island lipase at room temperature for 15 to 30 hours, filtered through a filter paper, And may be an extract obtained by concentration under reduced pressure.

At this time, eight antimicrobial active substances were obtained from the extract of the island of Gyorincho methanol, and the kinds thereof were gallic acid, methyl gallate, 3,6-di-O-galloyl arbutin (3 , 6-di-O-galloylarbutin), 2,6-di-O-galloylarbutin, 2,4,6-tri-O-galloyl- 2, 4,6-tri-O-galloyl-β-glucose, 1,3,4,6-tetra-O- galloyl- beta-glucose), 1,2,4,6-tetra-O-galloyl-beta-glucose, 1,2,3,6 -Tetra-O-galloyl-beta-glucose. ≪ / RTI >

Generally, the island is a perennial herbaceous plant belonging to the family of the stems of the genus Gemini. It has been used in civilian medicine for the treatment of diseases such as cancer, inflammation, wound and hypotension, and is known to have radioprotective effect and vasodilation effect. In addition, the islets in the genus Tyrannus contain flavonoids, phenolic acids, mainly gallic acids, coumarins, alkanoids, carbohydrates, polysaccharides, tannins, ) Are contained.

(Yucca extract)

Yucca is a plant belonging to the family Agaricus, which grows in North and South America. It is used for civilian medicine and food such as rheumatoid arthritis, and it is approved by US Food and Drug Administration (FDA) for use as natural edible food. These yucca can be easily cultivated or harvested in large quantities, and thus can be purchased at a very low cost.

According to the present invention, the yucca extract can be obtained by using a hot water extracting method using distilled water or a solvent extracting method using an organic solvent, and preferably a hot water extracting method can be used. That is, 800 to 1000 g of purified water or organic solvent is added to 50 to 150 g of yucca dried powder, and the mixture is extracted at 70 to 80 ° C for 1 to 3 hours, followed by filtration to obtain a low concentration extract of yucca. Ethyl acetate, acetone, methanol, chloroform, buthanol, etc., which are generally used for extracting natural products, may be used as the organic solvent. Ethanol, Is most suitable.

(Vitamin B1 lauryl sulfate)

Vitamin B1 Dilurylsulfate (C ₃₆ H ₆₈ O ₉ N ₄ S ₃ .H ₂ O) is not well soluble in water, it dissolves well in alcohol and chloroform and may be difficult to dissolve in ether and acetone . It also forms sols in warm water and can mix well with oils and fats with a powerful surfactant action.

In general, vitamin B1 lauryl sulfate has antifungal activity by inhibiting growth by attaching to the mycelial cell wall of fungi.

(Sorbic acid)

Sorbic acid (C ₆ H ₈ O ₂ ) is a colorless or white crystal and crystalline powder that is insoluble in water and soluble in alcohol, glacial acetic acid, acetone, ether and propylene glycol. It is relatively stable to heat or light but it can sublimate by heating and drying.

Sorbic acid inhibits growth of microorganisms such as filamentous fungi, yeast, and aerobic bacteria, but the sterilizing power may be weak. In addition, since the antimicrobial effect increases as the pH is acidic, it is preferable to use it at pH 6.0 or less.

(Benzoic acid)

Benzoic acid (C ₆ H ₅ COOH) is one of the simplest aromatic carboxylic acids and is a colorless crystalline solid. Benzoic acid is difficult to dissolve in cold water, but it can dissolve well in warm water and dissolves well in organic solvents such as acetone, ethanol, and ether, but it may not dissolve in petroleum ether.

According to the present invention, an emulsifying agent and an excipient may be used in addition to a mixture of at least one selected from the group consisting of an extract of the island giraffe and Yucca extract, vitamin B1 lauryl sulfate, sorbic acid and benzoic acid. As the emulsifying agent, a glycerin fatty acid ester may be used. As the excipient, Tween 20, glycerin, 1,3-butylene glycol, ethanol, hexylene glycol, propylene glycol may be used.

(Glycerin fatty acid ester)

Glycerin Esters of Fatty Acids are esters and derivatives of fatty acids and glycerine or polyglycerin, and examples thereof include glycerin fatty acid esters, glycerin acetic acid fatty acid esters, glycerin lactic acid fatty acid esters, glycerin citric acid fatty acid esters, glycerin succinic acid fatty acid Esters, glycerin diacetyltartaric acid fatty acid esters, glycerin acetic acid esters, polyglycerin fatty acid esters and polyglycerin condensed ricinoleic acid esters can be used, and glycerin fatty acid esters can be preferably used. At this time, the glycerin fatty ester may be used as an emulsifier because it has plasticity by including an acetyl group to form a thin film.

The glycerin fatty acid ester is not soluble in water but is soluble in an organic solvent such as alcohol, ethyl acetate, ether, and chloroform. Therefore, it is preferable to use an organic solvent to dissolve the glycerin fatty acid ester.

In the present invention, the antimicrobial raw materials produced according to the above-mentioned manufacturing method of antibacterial raw materials may be included in cosmetics, wet tissues or household goods. Here, the household article may be a sanitary article such as a diaper, a sanitary article, a mouth tissue, an oral cleanser, a shampoo, a soap, a shower gel, or the like.

At this time, the antibacterial raw material may be used in a wet tissue by adding 0.3 to 3 parts by weight of an antibacterial raw material to 100 parts by weight of distilled water to prepare a wet tissue liquid. In addition, the antibacterial raw material may be used as a cosmetic product by adding 0.5 to 5 parts by weight of the antibacterial raw material to 100 parts by weight of the cosmetic formulating composition to prepare a cosmetic raw material.

(Example of a wet tissue to which an antibacterial raw material is added)

Example 1.

Twenty 20% by weight of twenty 20% by weight of isomeric ginseng extract and 5% by weight of yucca extract were mixed, heated at 40 to 80 ° C for 20 minutes, 40% by weight of glycerin fatty acid ester was further added to 40 to 80 ° C for 20 minutes Heat it.

After that, Tween 20 containing glycyrrhizin extract and yucca extract and glycerin fatty acid ester are mixed to produce antibacterial raw material.

Examples 2 to 14

The antibacterial raw materials were prepared in the same manner as in Example 1 except that the contents shown in Table 1 were used. When vitamin B1 lauryl sulfate, sorbic acid or benzoic acid is used, it is added to glycerol fatty acid ester heated at 40 to 80 ° C for 20 minutes so that it can be mixed while being melted. Thereafter, it can be mixed with tween 20 containing the island giraffe extract or Yucca extract.

(Example of a cosmetic product to which an antibacterial raw material is added)

Examples 15 to 18

The antibacterial raw materials were prepared in the same manner as in Example 1 except that the contents shown in Table 1 were used. At this time, hexylene glycol or propylene glycol was used instead of Tween 20, and in the case of Examples 17 to 18, hexylene glycol or propylene glycol was used instead of benzoic acid and tween 20 in place of sorbic acid .

Island Kirincho
extract
yucca
extract Vitamin B1
Lauryl sulfate
Sorbic acid
Benzoic acid glycerin
fatty acid
ester Excipient Twin 20 Hexylene glycol Propylene glycol Example 1 20 5 - - - 40 35 - - Example 2 20 10 - - - 40 30 - - Example 3 20 - 5 - - 40 35 - - Example 4 20 - 10 - - 40 30 - - Example 5 20 - - 5 - 40 35 - - Example 6 20 - - 10 - 40 30 - - Example 7 20 10 5 - - 40 25 - - Example 8 20 10 10 - - 40 20 - - Example 9 20 10 - 5 - 40 25 - - Example 10 20 10 - 10 - 40 20 - - Example 11 20 - 10 5 - 40 25 - - Example 12 20 - 10 10 - 40 20 - - Example 13 20 10 5 5 - 40 20 - - Example 14 20 10 10 10 - 40 10 - - Example 15 20 10 5 5 - 40 - 20 - Example 16 20 10 5 5 - 40 - - 20 Example 17 20 10 5 - 5 40 - 20 - Example 18 20 10 5 - 5 40 - - 20

Experimental Example 1. Flotation test of a wet tissue including an antibacterial raw material

The wet tissue solution was prepared by mixing 0.3 wt% of the antibacterial raw material with 99.7 wt% of distilled water. Wet tissue (70% of rayon and 30% of polyester) was wetted with the prepared wet tissue to 3.5: 1, and then 7 kinds of microorganism strains were inoculated at 1 x 10 < ~ ⁵ > The seven strains of the microorganisms were Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Burkholderia cepacia ATCC 25416, Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404.

The antiseptic activity was confirmed by observing the bacterial kill rate with Petri film (3M) every week while keeping the microbial strain inoculated in a sample bag and storing it at 30 ° C for 28 days. The buoyancy test was conducted according to the test method of microbiological guidelines of the Cosmetic, Toiletry, and Fragrance Association (hereinafter referred to as CTFA).

The effectiveness of the buoyant force according to the CTFA standard should be such that the number of bacteria is reduced by more than 99.9% within 7 days after inoculation and there should be no proliferation during the test period. Yeast and fungi should also have at least 90% reduction in microbial counts within 7 days of inoculation, and the number of microbes must be reduced continuously during the test.

FIG. 1 (a) is a photograph of a wet tissue for the 28th day of inoculation, and FIG. 1 (b) is a photograph of a wet tissue for the 28th day of inoculation, and Table 2 shows the number of bacteria, yeast, mold, .

Number of bacteria
(CFU / ml) Yeast / Mold Number
(CFU / ml) Buoyancy
(Death rate ^** ) Example 1 TNTC ^* TNTC 0 % Example 2 TNTC TNTC 0 % Example 3        TNTC TNTC 0 % Example 4 TNTC TNTC 0 % Example 5 TNTC TNTC 0 % Example 6 TNTC TNTC 0 % Example 7 TNTC TNTC 0 % Example 8 TNTC TNTC 0 % Example 9 TNTC TNTC 0 % Example 10 TNTC TNTC 0 % Example 11 2 - > 99.99% Example 12 - - 100% Example 13 - - 100% Example 14 - - 100%

* TNTC (too many to count): too many to count

** Dying rate: (number of viable cells / number of inoculated cells) × 100

Table 2 shows the wetting strength of the wet tissue according to each of Examples 1 to 14. As a result, in Examples 11 to 14, the antibacterial activity was 99% or higher, the number of bacteria, and the number of yeast / molds were almost not measured, and thus the excellent preservative activity was obtained when all of the island giraffin extract, vitamin B1 lauryl sulfate and sorbic acid were contained Able to know. It is also confirmed that Examples 13 and 14 containing yucca extract have excellent repellency and that when sorbic acid is contained in an amount of 5% by weight or less, precipitation and precipitation occur less frequently.

EXPERIMENTAL EXAMPLE 2 Cosmetic Buffer Strength Test of Antibacterial Material

Experimental Example 2-1. Manufacture of cosmetics containing antibacterial raw materials

A basic cosmetic formulation composition was prepared by mixing 3 wt% of mineral oil, 2 wt% of stearic acid (45%), 0.6 wt% of polysorbate 60, 0.4 wt% of glyceryl stearate, 5 wt% of glycerin, 0.3 wt% of triethanolamine, 88.6% by weight, and 0.1% by weight of a carboxyl vinyl polymer (hereinafter referred to as basic cosmetic formulation composition).

A cosmetic product containing antibacterial raw materials is prepared by mixing 0.5% by weight of the antibacterial raw materials of Examples 15 to 18 with 99.5% by weight of the basic cosmetic formulation composition.

Experimental Example 2-2. Preservative effect of cosmetics containing antibacterial raw materials

Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Burkholderia sephacia, Candida albicans, and Aspergillus niger were cultured, and the cultured bacteria were cultured at 1 × 10 ^-5 CFU / g in the cosmetic containing the antibacterial raw material prepared in Experimental Example 2-1. Changes in bacterial counts were observed for 1 to 28 days after inoculation with the strain, and this experiment was conducted according to the test method of the CTFA microorganism guideline.

2 (a) to 2 (c) show the results of the flotation test of cosmetics containing only the basic cosmetic formulation composition containing no antibacterial raw material. As the number of days of inoculation increased, the number of microorganisms increased to more than Log 6, It can be confirmed that it is proliferated.

On the other hand, the hexylene glycol graph of FIG. 2 (a) shows that the antibacterial raw material containing the antibacterial raw material according to Example 15 was tested by the flotation test, and the number of microorganisms continuously decreased over the number of inoculation days, And it can be confirmed that it is not proliferated again after it is reduced. In the propylene glycol graph of FIG. 2 (b), the same results as those of FIG. 2 (a) can be confirmed by a flotation test of cosmetics containing the antibacterial raw material according to Example 16.

The Methylparaben graph of FIG. 2 (c) shows that the microbial counts gradually decreased over the inoculation days of 99.84% by weight of the basic cosmetic formulation composition and 0.16% by weight of the conventional chemical preservative, Methylparaben, 0 < / RTI >

As shown in Fig. 2, the number of microorganisms decreased to Log 0 as a result of the inoculation day 7 of each of the cosmetic products containing the antibacterial raw material composed of the island giraffe extract and the food additive, and the killing rate of 100% The excellent anti-buoyancy of cosmetics containing raw materials can be found.

Example 3. Patch test of antibacterial raw material

30 healthy adults consisting of 17 female and 13 male subjects were tested and the degree of skin irritation was confirmed by the test of the antimicrobial raw material containing the extract of the present invention and the food additive.

Saline solutions containing 5% by weight of the antibacterial raw materials of Examples 15 to 18 were prepared, respectively, and 10 μl of the saline solution was absorbed to a paper disk. The saline solution was applied to the lower part of the arm of 30 subjects and then examined for patches after 24 hours and 48 hours , Which is shown in Table 3 below.

division 24 hours 48 hours ++++ +++ ++ + - ++++ +++ ++ + - Example 15 - - - - 30 - - - - 30 Example 16 - - - - 30 - - - - 30 Example 17 - - - - 30 - - - - 30 Example 18 - - - - 30 - - - - 30

++++: For algebra case (weight 4.0)

+++: marked erythema, papules and blisters (weighted 3.0)

++: marked erythema but weak erythema, swelling and swelling (weight 2.0)

+: Faint erythema (weight 1.0)

-: no reaction with no flare (weight 0.0)

As shown in Table 3 above, the results of the test for the test pieces of 30 test subjects were confirmed. As a result, it was confirmed that the antibacterial raw materials of Examples 15 to 18 were not skin irritant. In addition, the mean response was calculated by the following formula.

As shown in Table 3, the average skin response was calculated to be 0.0 and the average skin response value was 0 because the skin was not reacted with no flare from the subject. At this time, the above criteria for determining the average skin reactivity can be judged as unstimulated when the average skin reactivity value is 0.0 to 0.9, mild stimulation when 1.0 to 2.9, moderate stimulation when 3.0 to 4.9, and strong stimulus when it is 5.0 or more. Therefore, by confirming that the antibacterial raw material of the present invention is within the non-stimulated range, it can be confirmed that the antibacterial raw material is safe for human body.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (5)

In an antibacterial raw material using an island giraffe extract,
The antimicrobial raw material comprises a) an island giraffin extract and
b) an antibacterial raw material characterized by containing at least one selected from the group consisting of yucca extract, vitamin B1 lauryl sulfate, sorbic acid, benzoic acid, and mixtures thereof.
The method according to claim 1,
Wherein the weight ratio of a) to b) is 1: 0.4 to 1: 1.5.
The method according to claim 1,
Wherein the antimicrobial raw material is added to the wet tissue liquid in an amount of 0.3 to 3 parts by weight per 100 parts by weight of distilled water.
The method according to claim 1,
Wherein the antimicrobial raw material is added to 0.5 to 5 parts by weight of the antimicrobial raw material in 100 parts by weight of the cosmetic formulating composition to be contained in the cosmetic raw material.
The method according to claim 1,
Wherein the antibacterial raw material is contained in a raw material for daily use.

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