KR20080100095A - Cosmetic composition for improving the acne containing lactobacillus rhamnosus or its culture medium as active ingredient - Google Patents

Abstract

A cosmetic composition for improving acne is provided to have an excellent moisturizing effect and no skin irritation, and to remove pimple using culture fluid of the Lactobacillus rhamnosus lactic bacteria. A cosmetic composition for improving acne comprises Lactobacillus rhamnosus lactic bacteria or a culture fluid of the Lactobacillus rhamnosus lactic bacteria as an active ingredient. The Lactobacillus rhamnosus lactic bacteria is a Lactobacillus rhamnosus strain PL 60(KCCM-10654P) and is raw bacteria, dried bacteria or killed organism. The cosmetic composition suppresses growth of acne-causing bacterium which is Propionibacterium acnes.

Description

Cosmetic composition for improving acne containing Lactobacillus rhamnosus lactic acid strain or its culture solution {Cosmetic Composition for Improving the Acne containing Lactobacillus rhamnosus or its Culture Medium As Active Ingredient}

The present invention relates to a cosmetic composition for improving acne, and more particularly to a cosmetic composition containing Lactobacillus rhamnosus lactic acid strain or its culture solution.

The development of acne begins with the abnormal action of testosterone, the male hormone of our human hormones, which flows into the sebaceous glands of the skin and promotes the production of oil, which is excreted in the sebum pores. Filled up. At this time, with excessive production of oil, the inlet of pores is accelerated, and the pores of the pores are narrowed or blocked, which causes the oil that fills the pores to stop flowing out and become trapped in the pores. Lumps and other impurities entangle each other to form a pimple. As the amount of oil stagnated in the pores increases, the bacteria, especially Propionibacterium acnes, are active, and free fatty acids, a by-product of the bacteria's devouring of the oil components, enter the pores. Severe irritation causes inflammation.

In general, in the clinical treatment of skin diseases such as acne and inflammation caused by bacteria, antibacterial substances such as erythromycin, tetracycline, and sindamycine have been mainly used. However, these antimicrobial agents have caused many problems such as acne causing bacteria have long-term resistance to antibiotics, causing side effects such as photosensitivity. In addition, propioni bacterium acnes, the cause of acne, was difficult to artificially cultivate, making it difficult to select an appropriate drug. In addition, there was a problem that antibiotics were mainly used as cosmetics.

The antimicrobial substances produced by microorganisms have the ability to inhibit a wide range of microorganisms and have low selective toxicity to higher organisms. Therefore, a method of using a metabolite of lactic acid bacteria having antimicrobial activity for the treatment of skin diseases has been sought. Metabolites of lactic acid bacteria are harmless to the human body and are easily decomposed by protease secreted from the human body. However, in the process for obtaining lactic acid bacteria metabolites, there is a disadvantage that the cost of the substrate used is high, and high cost is required for the separation and purification of metabolites.

* Recently, Japanese Patent Laid-Open No. 2003-259860 describes a cosmetic composition containing Lactococcus lactis and an antimicrobial culture solution using the same, and Korean Patent No. 0536522 discloses a cosmetic composition containing Enterococcus fascium and a bacteriocin using the same. It is described that the situation is developing lactic acid bacteria and its culture solution as a cosmetic composition.

Accordingly, the present inventors have made intensive studies to overcome the problems of conventional cosmetic compositions for acne, and as a result, the composition containing Lactobacillus rhamnosus strain or its culture solution is excellent as an inflammatory skin disease cosmetic such as acne, and skin irritation is The present invention was completed by confirming that the moisturizing effect was excellent.

Accordingly, an object of the present invention to provide a cosmetic composition for improving acne containing Lactobacillus rhamnosus lactic acid strain or a culture thereof.

Other objects and advantages of the invention will be apparent from the following examples and claims.

The present invention relates to a cosmetic composition for improving acne containing Lactobacillus rhamnosus lactic acid strain or a culture thereof.

Hereinafter, the present invention will be described in detail.

In order to achieve the above object, the cosmetic composition of the present invention is added to the cosmetic composition culture medium cultured Lactobacillus rhamnosus lactic acid strain or Lactobacillus rhamnosus lactic acid strain as an active ingredient.

The culture solution can be obtained by culturing Lactobacillus rhamnosus lactic acid strain in a general microbial strain culture medium. In addition, the culture medium may be used for all culture medium including the culture medium or the lactic acid bacteria cultured to remove the lactic acid strain by centrifuging the culture medium cultured Lactobacillus rhamnosus lactic acid strain.

Lactobacillus rhamnosus strains used in the present invention include all of the Lactobacillus rhamnosus strain, preferably, Lactobacillus rhamnosus strain PL60 (L. rhamnosus Strain PL6O). In addition, Lactobacillus rhamnosus lactic acid strains can be used both live, dry or dead bacteria.

The cosmetic composition of the present invention may inhibit the growth of acne causing bacteria and inhibit the occurrence of acne, and has an antibacterial function against acne causing bacteria. Acne causing bacteria in the above include, for example, Propionibacterium acnes.

The Lactobacillus Lactobacillus rhamnosus strain PL6O (L. rhamnosus Strain PL6O) was internationally deposited as KCCM-10654P at the Korea Microbiological Conservation Center on May 12, 2005.

The content of Lactobacillus rhamnosus lactic acid strain or culture medium thereof of the present invention is 0.00001% to 50% by weight relative to the total weight of the cosmetic composition, preferably 0.001 to 10% by weight.

When the cosmetic composition of the present invention contains Lactobacillus rhamnosus lactic acid strain as an active ingredient, lactic acid strain of dry weight of 1 × 10 ^4-1 × 10 ¹¹ CFU / g to 0.00001 to 50.0% by weight relative to the total weight of the cosmetic composition It includes, preferably contains the dry weight of the lactic acid strain 0.001 to 10% by weight. In addition, when the cosmetic composition of the present invention comprises a culture solution of Lactobacillus rhamnosus lactobacillus as an active ingredient, the culture solution in a state in which the lactic acid strain is incubated in a confluence state, preferably 0.00001 to 50.0% by weight, 0.001 to 10% by weight.

At this time, when the lactic acid strain or culture medium in the cosmetic composition is less than the minimum content, no obvious effect can be expected, and when the maximum content is exceeded, the acne skin improvement effect does not increase any more and is likely to cause irritation to the skin. It also has a big impact on stabilization.

Ingredients contained in the cosmetic composition of the present invention inhibits the formation of skin acne, excellent stability of the cosmetic itself, and is a product by biosynthesis, there is no skin irritation or other side effects, it is safe and has an excellent moisturizing effect, etc. It is not limited to. In addition to the above components, it may also include components conventionally used in cosmetic compositions, and include conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing It may be formulated as, oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, when the composition of the present invention is used as a cosmetic composition, a basic cosmetic selected from lotion, emulsion, cream, essence, gel, pack and cleansing cream; It may be prepared in a formulation selected from the group consisting of makeup cosmetics such as foundation.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, Crystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Experimental Example  One: Lactobacillus Rhamnosus  Cells and Culture Media Preparation

1 × 10 ⁶ cfu / l each of Lactobacillus rhamnosus strain PL60 (KCCM-10654P) strains in 1 L of culture medium prepared with 10.0 wt% brown rice, 1.0 wt% tryptone, 0.2 wt% yeast extract, 0.1 wt% Tween 80 and distilled water Inoculated with ㎖ incubated for 24 hours to obtain a culture. The obtained Lactobacillus rhamnosus strain culture solution was centrifuged at 5,000 x g for 30 minutes to separate cells and culture medium, and the cells were lyophilized and powdered.

Experimental Example 2: Lactobacillus Rhamnosus Growth Inhibition Effect of Acne Strains According to the Content of PL60 and Its Culture Solution

Growth inhibitory effect on the acne strains was tested using the cell powder and culture medium of the Lactobacillus rhamnosus PL60 strain obtained in Experimental Example 1.

Growth inhibition experiment on acne strains was performed using Propionibacterium acne (ATCC 6919) as the cause of acne, and Lactobacillus rhamnosus cells and cultures were diluted to 0.001%, 0.01% and 0.1%, respectively. The experiment was carried out by the paper disk method. To describe this in detail, first, 2% RCM medium (Reinforced clostridial medium, Difco) was prepared and placed in 87 x 15 mm Petri dish (green cross) to make a solid medium, and propionibacterium acnes 1 × 10 ⁶ cells were smeared. After that, the paper disk (8mm) was put on, and the disk was inoculated with 50 μl of Lactobacillus cells and dilutions of the culture solution. The inoculated solid medium was placed in an anaerobic incubator and incubated at 37 ° C. for 4 days, and then the clear zone was observed. The results are shown in Table 1.

Lactobacillus  Growth Inhibitory Effects on Acne Strains and Culture Medium content Propionibacterium acnes growth hypotonic ring (diameter, mm) Lactobacillus rhamnosus PL60 Cell Powder 0.001% Solution 10 Cell powder 0.01% solution 18 Cell powder 0.1% solution 28 Lactobacillus rhamnosus PL60 culture 0.001% 7 0.01% of culture 16 0.1% culture 25 Erythromycin (0.2%) 29

As can be seen from the results of Table 1, both Lactobacillus rhamnosus PL60 cell powder and culture medium showed growth inhibition effect against the acne-causing bacteria, the effect of the cell powder was higher, the concentration in both the cell powder and culture medium Dependent on the growth inhibition effect.

Experimental Example 3: Lactobacillus Temperature Change Stability Test of Cosmetic Formulations Containing Rhamnosus Cells and Cultures

Formulation containing Lactobacillus rhamnosus strain PL60 cell powder or culture medium of Experimental Example 1 according to the composition of Table 2 using the composition commonly used in the industry to test the stability according to the temperature change of the cosmetic formulation of the present invention Were prepared.

ingredient Content (% by weight) Comparative Example 1 Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Comparative Example 1 Lactobacillus rhamnosus strain PL60 cell powder of Experimental Example 1 - 5.0 10.0 50.0 - - - - Lactobacillus rhamnosus strain PL60 strain culture medium of Experimental Example 1 - - - - 5.0 10.0 50.0 - Erythromycin - - - - - - - 0.2 Hydrophilic Monoglycerol Stearate 2.0 Cetearyl Alcohol 2.2 Stearic acid 1.5 Beeswax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Cured Vegetable Oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 incense 0.01 Uniside-U13 0.02 Green # 3 0.00 Distilled water Remaining amount Sum 100

Formulation Examples 1, 2, and 3 containing Lactobacillus rhamnosus cells of the present invention, and Formulation Examples 4, 5, and 6, containing Lactobacillus rhamnosus strain culture solution, together with Control Example 1 and Comparative Example 1, were placed in a vial container. After storage for 1 week in a constant temperature bath at 45 ℃, also kept in an opaque glass jar at a constant 4 ℃ and stored in a completely shaded refrigerator for 1 week, the degree of discoloration was measured according to Table 3 The results are shown in Table 4 below.

Product discoloration evaluation criteria score Evaluation standard 0  No change One  Very little change 2  Little change 3  Slightly worse 4  Badly changed 5  Extremely severe

Results of stability test of the formulation Temperature Discoloration degree of test substance Comparative Example 1 Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Comparative Example 1 45 ℃ 0 0 0 0 0 0 0 0 4 ℃ 0 0 0 0 0 0 0 0

As can be seen from the results of Table 4, the formulations did not discolor in Formulations 1 and 2 and Formulation Examples 4, 5 and 6, except for Formulation Example 3 containing 50.0% of the Lactobacillus rhamnosus bacteria cells 50.0 Lactobacillus cells 50.0 It was found that the stability of the formulation containing less than% is excellent.

Experimental Example 4: Lactobacillus Human safety testing of formulations containing Rhamnosus cells or cultures

The Lactobacillus rhamnosus bacterium of Experimental Example 3 (see Table 2) was 5.0 wt% in 40 women in their 20s and 30s who had no hypersensitivity to skin irritation due to past history and had no skin disease or skin allergy symptoms. The skin safety of the cosmetic formulation of Formulation Example 1 containing Formulation Example 4 containing 5.0% by weight of Lactobacillus rhamnosus culture solution was confirmed by a human skin patch test. For comparison, Comparative Example 1 containing 0.2% by weight of erythromycin and Control Example 1 containing nothing were tested together.

First, wipe the test area with 70% ethanol and dry it. Then, drop the prepared test substance into 15 μg of Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland), and then inside the forearm of the subject. Airtightly wrapped. The patch was applied for 24 hours, the patch was removed, and the test site was marked with a marker pen. 1 hour and 24 hours after each removal (1 hour after removal, 24 hours = 24 hours, 48 hours including patch attachment) using a magnifying glass (8MC-150, DAZOR, United States) to observe the test site for erythema and edema Was observed.

The skin reaction was determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) (see Table 5), and the average skin reactivity was calculated by Equation 1 below and the results are shown in Table 6.

Evaluation criteria and score of skin reaction Symbol score Evaluation standard - 0 No response, normal skin ± 0.5 Faint or light erythema + 1.0 Boundary but weak erythema, edema and papules ++ 2.0 Pronounced erythema, papules and vesicles +++ 3.0 Severe erythema and vesicle formation ++++ 6.0 Cannon formation

Human skin patch test result Test substance 24 hours 48 hours Average skin reactivity (n = 20) ± + ++ ± + ++ Comparative Example 1 2 - - --- 0.41 Formulation Example 1 (Cellulum Powder 5.0%) 2 - - --- 0.41 Formulation Example 4 (5.0% culture solution) 2 - - --- 0.41 Comparative Example 1 4 2- One - - 1.87

As can be seen from the results of Table 6, the formulation of Comparative Example 1 containing 0.2% of erythromycin has low skin safety, while Formulation Example 1 and Lactobacillus rhamnosus containing 5.0% by weight of Lactobacillus rhamnosus cells Formulation Example 4 containing 5.0% by weight of the culture medium obtained the same skin reactivity value as that of Control Example 1, which contained nothing, thereby reducing skin safety in all formulations containing 5.0% by weight of Lactobacillus rhamnosus cells and culture medium. It was found to be very good.

Experimental Example  7: Lactobacillus  Moisturizing effect test of formulation containing cells

Formulation Example 1 containing Comparative Example 1, Comparative Example 1 of the Experimental Example 3 (Table 2) and Lactobacillus rhamnosus cells and the culture medium of the present invention in a healthy 20-30-30 women in 5.0 wt% The moisturizing effect of Formulation Example 4 was measured.

Each group was divided into two groups of 40 people, and the formulations of Comparative Example 1, Comparative Example 1, Formulation Example 1, and Formulation Example 2 were given, respectively, and applied to the face and forearm twice a day for 1 month. Immediately before applying the cosmetics, skin conductivity was measured in advance using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) at constant temperature and humidity conditions (21 degrees, 55% humidity), and the default value (0) was used. The objective effect was evaluated by measuring the change in skin moisture after 1 week, 2 weeks, 4 weeks and 2 weeks after application stoppage (after 6 weeks), and the results are shown in Table 7 below.

Human skin moisturizing effect test result Skin moisture growth rate (%) 1 week later after 2 weeks 4 weeks later 6 weeks later Comparative Example 1 15 16 21 22 Formulation Example 1 (Cellulum Powder 5.0%) 41 47 55 61 Formulation Example 4 (5.0% culture solution) 38 45 52 58 Comparative Example 1 16 19 25 30

As can be seen from the results of Table 7, Formulation Example 1 and Formulation Example 4 containing 5.0% by weight of the Lactobacillus rhamnosus cells and the culture medium of the present invention, respectively, containing Comparative Example 1 and erythromycin containing 0.2% Compared with Comparative Example 1, it was found that the skin skin moisturizing effect is much superior.

The cosmetics containing the Lactobacillus cells or the culture medium thereof according to the present invention not only inhibit acne production by inhibiting the growth of microorganisms propionibacterium acnes and bacteria that are involved in acne causing skin, but also inhibit the stability of the formulation. It is excellent, has no skin irritation, and shows an excellent moisturizing effect.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Therefore, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (8)

Acne improvement cosmetic composition containing Lactobacillus rhamnosus lactic acid strain or its culture solution as an active ingredient. The cosmetic composition according to claim 1, wherein the Lactobacillus rhamnosus lactic acid strain is Lactobacillus rhamnosus strain PL60 (KCCM-10654P). The cosmetic composition according to claim 1, wherein the Lactobacillus rhamnosus lactic acid strain is live, dry or dead. The cosmetic composition according to any one of claims 1 to 3, wherein the cosmetic composition inhibits the growth of acne causing bacteria, has an antibacterial function against acne causing bacteria, has skin safety, and has a moisturizing function. . The cosmetic composition according to claim 4, wherein the acne causing bacterium is propionibacterium acnes. The cosmetic composition according to claim 1, wherein the cosmetic composition contains a lactic acid strain of 1 × 10 ⁴ -1 × 10 ¹¹ CFU / g of dry weight in an amount of 0.00001-50.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the lactic acid strain culture medium is contained in an amount of 0.00001-50.0 wt% based on the total weight of the cosmetic composition. The composition of claim 1 wherein the cosmetic composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition, characterized in that it has a formulation selected from the group.