KR102217554B1 - Sedum takesimense extract carbornhydrate nanocomposite and method for producing the same - Google Patents

Abstract

The present invention relates to a carbohydrate nanocomposite of a sumirincho extract and a method for preparing the same, comprising a carbohydrate nanocomposite of a sumirincho extract, dextrin and an emulsifier, and (a) a complex combining the extract and an emulsifier with a water Dissolving in; (b) dissolving dextrin in an organic solvent; (c) mixing the solution prepared in step (a) and step (b); (d) removing the organic solvent from the mixed solution through the step (c); it provides a method for producing a carbohydrate nanocomposite of the extract of seomgirincho, characterized in that it comprises.
According to the present invention as described above, by combining the sumirincho extract with carbohydrates to form a complex of nano-sized particles, the sumirincho extract has higher solubility and stability when used by adding it to foods or cosmetics rather than the existing sumirincho extract itself. Composites can be provided.

Description

Sumirincho extract carbohydrate nanocomposite and its manufacturing method. {SEDUM TAKESIMENSE EXTRACT CARBORNHYDRATE NANOCOMPOSITE AND METHOD FOR PRODUCING THE SAME}

The present invention relates to a carbohydrate nanocomposite of a sumirincho extract and a method for preparing the same, and more particularly, by combining a sumirincho extract with dextrin, etc. to form a complex of nano-sized particles, by adding the existing sumirincho extract itself to foods or cosmetics, etc. It provides a higher solubility and stability compared to the case of use, and it relates to a sumirincho extract complex that enables the various effects of the sumirincho to be exhibited well.

Sedum Kantschaticum is a perennial plant belonging to the sedum family. It has a round column shape and is about 30-50 cm tall. The flower blooms in June-July, and the fruit is 5 goldolaceae, and when ripe, it breaks into a star shape. It grows wildly on the rocks of mountains and fields, but is mainly distributed in Ulleungdo and Dokdo in Korea. Kirincho extract is known to have several effects, and is known to exhibit antibacterial or antiviral effects, anti-inflammatory and antioxidant effects, skin whitening effects, and antimalarial effects.

Regarding the antibacterial effect, preservatives are essential in foods, cosmetics, and medicines to prevent spoilage by microorganisms and increase product stability. Due to the nature of the product, the shelf life is relatively long, and this is especially the case for cosmetics with many nutrients from microorganisms. However, paraben-type preservatives that are commonly used in conventional cosmetics and pharmaceuticals have a problem that causes skin allergies. In addition, food preservatives also have new problems such as acute/chronic toxicity and mutagenesis caused by continuous accumulation in the body. Therefore, in order to solve this problem, methods of using natural antibacterial substances such as alkaloid, flavonoid, phytoalexin, and antibacterial peptide as preservatives have emerged, but in most cases of these natural antibacterial substances There is a problem that is difficult to commercialize due to a decrease in pH, a narrow antibacterial spectrum, and a problem in the formulation.

In addition, with regard to the antioxidant effect, natural antioxidants such as tocopherol and vitamin C are widely used in foods, medicines and cosmetics because they are safe for the human body, but in reality synthetic antioxidants are mainly used because of their low stability and low antioxidant capacity and economical efficiency. have. However, the use of synthetic antioxidants is legally regulated due to concerns about safety such as side effects on the human body.

Regarding skin whitening, cosmetics have previously used ascorbic acid, kojic adcid, arbutin or derivatives thereof to treat or alleviate excessive melanin pigmentation caused by exposure to UV rays. In addition, they have been used in combination with pharmaceuticals, but their use is limited due to insufficient whitening effect, safety problems for the skin, and stability problems in the formulation when formulated in cosmetics. Therefore, in order to solve the above problems, efforts are being made to develop substances that are safe for the human body and exhibit excellent antibacterial, antioxidant, or whitening effects, as well as excellent stability by using Sedum Takesimense (ST). have.

Korean Registered Patent Publication No. 10-1637167

It is an object of the present invention to form a complex of nano-sized particles by combining a sumirincho extract with dextrin, etc., thereby providing high solubility and stability compared to the case of adding and using the existing sumirincho extract itself to foods or cosmetics. It is to ensure that the various effects of

In order to achieve the above object, the present invention provides a carbohydrate nanocomposite of a sumirincho extract (ST), a dextrin (Dextrin) and an emulsifier.

The sumirincho extract carbohydrate nanocomposite may include the sumirincho extract, the dextrin, and the emulsifier in the same weight ratio of 1:1:1 to the weight of the sumirincho extract carbohydrate nanocomposite.

In order to achieve the above object, the present invention provides a method for preparing a nanocomposite of a carbohydrate extract from a sumirin herb, comprising the steps of: (a) dissolving a complex obtained by combining the extract and an emulsifier in water; (b) dissolving dextrin in an organic solvent; (c) mixing the solution prepared in step (a) and step (b); (d) removing the organic solvent from the mixed solution passed through step (c).

The method of preparing the seomgirincho extract carbohydrate nanocomposite may be further subjected to the step of removing water from the mixed solution that has undergone step (d) after step (d).

The method of preparing the sumirincho extract carbohydrate nanocomposite may include the sumirincho extract (ST), the dextrin, and the emulsifier in the same weight ratio of 1:1:1 to the weight of the sumirincho extract carbohydrate nanocomposite.

The organic solvent may be ethanol.

According to the present invention as described above, by combining the sumirincho extract with dextrin, etc. to form a complex of nano-sized particles, the sumirincho extract has higher solubility and stability when used by adding it to foods or cosmetics rather than the existing sumirincho extract itself. Composites can be prepared.

Figure 1 (a) shows the particle size (Particle size) and PDI (Poly disperse index) of the extract carbohydrate nanocomposite (ST-L10D-HPβCD) according to one embodiment of the present invention, Figure 1 (b) is Zeta It represents the potential (ζ-potential).
FIG. 2(a) shows the particle size and PDI when a carbohydrate nanocomposite (ST-L10D-HPβCD) was freeze-dried and dissolved in water at the same concentration, and FIG. 2(b) shows the zeta potential.
Figure 3(a) shows the particle size and PDI according to the concentration of the NaCl aqueous solution in which the extract carbohydrate nanocomposite (ST-L10D-HPβCD) is present, and Figure 3(b)s shows the zeta potential, and Figure 3(c) Indicates whether a precipitate was formed when left in NaCl aqueous solution for 12 hours.
Figure 4 (a) shows the particle size and PDI according to the pH change of the extract carbohydrate nanocomposite (ST-L10D-HPβCD), Figure 4 (b) is the zeta potential, Figure 4 (c) is 12 after pH adjustment. It indicates whether or not it precipitates when left for a while.
Figure 5 shows the DPPH radical scavenging capacity (diphenyl-2-picrylhydrazyl radical-scavenging capacity) according to the concentration of the sumirincho extract (ST) and the sumirincho extract carbohydrate nanocomposite (ST-L10D-HPβCD).

Hereinafter, an embodiment of the present invention will be described in detail.

The sumirincho extract carbohydrate nanocomposite according to an embodiment of the present invention includes a sumirincho extract (ST), dextrin and an emulsifier. The dextrin may be hydroxypropyl-β-cycliodextrin (HP-β-CD). The HP-β-CD is a substance that is also listed on the food safety list, GRAS (generally recognized as safe), and corresponds to a substance that is safe for humans when ingested. The HP-β-CD has the following structure as a kind of carbohydrate.

In the case of the sumirincho extract carbohydrate nanocomposite, the ST is bound in the ring of the HP-β-CD, so that the HP-β-CD coats the ST. When ST is bonded into the ring of HP-β-CD, a number of OH groups, which are hydrophilic groups present outside the ring of HP-β-CD, enter the inside of the ring, and in the case of the combination of HP-β-CD and ST, the surface becomes hydrophobic. do. Decaglyceryl laurate (L10D) may be used as the emulsifier, and by further including L10D, L10D surrounds the surface of the conjugate of the HP-β-CD and ST, and the carbohydrate nanocomposite of the extract of the sesame rhizome ( ST-L10D-HPβCD) can exist in a dissolved state in water.

The sumirincho extract carbohydrate nanocomposite may include the sumirincho extract, the dextrin, and the emulsifier in the same weight ratio of 1:1:1 to the weight of the sumirincho extract carbohydrate nanocomposite. If too much of the sumirincho extract (ST) is used in excess of the same weight ratio, ST that cannot be combined with the dextrin may remain precipitated in the solvent, and too little is used below the same weight ratio. In some cases, the complex itself may not be well formed.

The method of preparing a nanocomposite of a carbohydrate extract of a sumirincho in accordance with an embodiment of the present invention comprises the steps of: (a) dissolving a complex combining the extract of sumirincho and an emulsifier in water; (b) dissolving dextrin in an organic solvent; (c) mixing the solution prepared in step (a) and step (b); (d) removing the organic solvent from the mixed solution passed through step (c). The organic solvent may be ethanol.

The dextrin may be hydroxypropyl-β-cycliodextrin (HP-β-CD). In the mixed solution after step (c), the ST is bonded to the ring of the HP-β-CD so that the HP-β-CD is coated with the ST. When ST is bonded into the ring of HP-β-CD, a number of OH groups, which are hydrophilic groups present outside the ring of HP-β-CD, enter the inside of the ring, and in the case of the combination of HP-β-CD and ST, the surface becomes hydrophobic. do. Decaglyceryl laurate (L10D) may be used as the emulsifier, wherein the L10D surrounds the surface of the conjugate of the HP-β-CD and ST, so that the extract carbohydrate nanocomposite (ST-L10D- HPβCD) can exist in a dissolved state in water.

In the case of step (d), the mixed solution that has passed through step (c) can be removed by evaporating ethanol, which is an organic solvent, with an evaporator set at 40°C according to the principle of Antisolvent co-precipitation. The giraffe extract (ST) and the complex (ST-L10D-HPβCD) in which the emulsifier and dextrin are bound are present in water as a solvent.

The method of preparing the seomgirincho extract carbohydrate nanocomposite may be further subjected to the step of removing water from the mixed solution that has undergone step (d) after step (d). In the case of the mixed solution after step (d), since the complex (ST-L10D-HPβCD) in which the extract (ST), emulsifier, and dextrin is conjugated in water is present, the mixed solution is freeze-dried to remove water. By doing so, the complex (ST-L10D-HPβCD) can be separated.

The method of preparing the sumirincho extract carbohydrate nanocomposite may include the sumirincho extract (ST), the dextrin, and the emulsifier in the same weight ratio of 1:1:1 to the weight of the sumirincho extract carbohydrate nanocomposite. More specifically, the weight of the sumirincho extract (ST) is 0.5%, the weight of the dextrin (Dextrin) is 0.5%, and the weight of the emulsifier is 0.5% relative to the total volume of the mixed solution passed through step (c). I can. If too much of the sumirincho extract (ST) is used in excess of the same weight ratio, ST that cannot be combined with the dextrin may remain precipitated in the solvent, and too little is used below the same weight ratio. In some cases, the complex itself may not be well formed.

Hereinafter, the present invention will be described in more detail through examples. These examples are for illustrative purposes only, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not construed as being limited by these examples.

Example

The complex of the extract and the emulsifier after removing the ethanol as shown below in the same weight ratio of the extract (ST), dextrin, and emulsifier to the weight of the carbohydrate nanocomposite of the extract of Sumirincho. And a sumirincho extract and emulsifier used so that the weight of the sumirincho extract (ST) is 0.5%, the weight of the dextrin (Dextrin) is 0.5%, and the weight of the emulsifier is 0.5%, based on the volume of the mixed solution including dextrin (Dextrin). The combined complex is dissolved in water and dextrin in an organic solvent, respectively. And the solutions are mixed. Thereafter, when ethanol is evaporated and removed from the mixed solution by using an evaporator at 40°C by an antisolvent co-precipitation method, the seomgirincho extract carbohydrate nanocomposite (ST-L10D-HPβCD) is precipitated in water as a solvent. Is formed.

Measurement Example 1.

By fixing the concentration of L10D and HPbCD and varying the concentration of ST, the particle size, PDI (poly disperse index), and zeta potential of the carbohydrate nanocomposite (ST-L10D-HPβCD) of Seogirincho extract Was measured.

As shown in Figure 1 (a), when the weight of the sumirincho extract (ST) is 0.4 to 0.5% compared to the volume of the mixed solution containing the complex of the extract and emulsifier and dextrin after removing ethanol, that is, When the ratio of ST, L10D, and HPbCD was 0.8:1:1 to 1:1:1, no precipitate was generated, and the smallest particle size and low PDI value were shown. In addition, when the weight of ST was 0.1 to 0.2%, the excess HPβCD that could not be combined with ST was precipitated, and when the weight of ST was 0.6 to 1.2%, the remaining ST could not be combined with HPβCD and precipitated.

In the case of a PDI value, it can be seen that it has a relatively uniform particle size at values less than 0.5, and a lower PDI value is considered to have a uniform particle size. Therefore, when the ratio of ST, L10D, and HPbCD is 0.8:1:1 to 1:1:1, it can be seen that the particle size of ST-L10D-HPβCD is the smallest and most uniformly present. The zeta potential is an index indicating how stable it can exist in an aqueous solution, and the larger the absolute value, the more stable it exists in the aqueous solution. In Fig. 1(b), there is a slight difference depending on the ST addition ratio (0.1 to 1.2%), but the overall absolute value is relatively large, and when the addition ratio of ST is 0.5% or more, the absolute value of the zeta potential is about 18. It shows a large value. Therefore, the stability of ST-L10D-HPβCD in aqueous solution seems not to be significantly affected by the ST addition ratio (0.1 to 1.2%).

Measurement example 2.

When a carbohydrate nanocomposite (ST-L10D-HPβCD) dissolved in water is freeze-dried and then dissolved again in water, the particle size increased compared to before freeze-drying, and the PDI value was lowered, resulting in a uniform particle size. In the case of the zeta potential (Fig. 2(a)), there is no significant difference, but the absolute value of the zeta potential is 15 V before freeze-drying, but about 23 V when re-dissolved after freeze-drying. As shown (Fig. 2(b)), it can be seen that the complex is stably present even when re-dissolved after freeze-drying in a solution. Therefore, if the carbohydrate nanocomposite (ST-L10D-HPβCD) is freeze-dried and commercialized in foods or cosmetics, it is generally more soluble in food than before freeze-drying and can still exist stably. It is expected to be more advantageous.

Measurement Example 3

When the concentration of the aqueous solution was varied by adding sodium chloride (NaCl) to the aqueous solution containing the extract carbohydrate nanocomposite (ST-L10D-HPβCD) from the extract of Sumirincho, the particle size did not show a significant change, such as about 8 to 15 nm, and NaCl It can be seen that the PDI value increases to a maximum of 1.0 until the concentration of the aqueous solution increases to 600 mM, and then gradually decreases at a higher concentration (Fig. 3(a)). Therefore, as the NaCl aqueous solution concentration increases, the PDI value of the complex increases to 0.5. Although there are cases where the abnormality is shown, it can be seen that the original shape of the composite is relatively well maintained since the size of the particle remains almost unchanged and the original particle size is maintained. Therefore, in the case of ST-L10D-HPβCD, it is believed that when used in foods or cosmetics having various salt concentrations, it can be dissolved in the form of nano-sized small particles and stably exist. In addition, the zeta potential of the complex in the aqueous solution generally increased with the change in the NaCl aqueous solution concentration than when the NaCl aqueous solution concentration was 0 mM, indicating that the complex was more stable in the NaCl aqueous solution. (Fig. 3(b))

In addition, when the concentration of the aqueous solution was changed by adding sodium chloride (NaCl) to the aqueous solution containing the extract carbohydrate nanocomposite (ST-L10D-HPβCD) from the sesame extract, no precipitate was observed when left for 12 hours. (Fig. 3(c))

Therefore, in the case of ST-L10D-HPβCD, when it is used in foods or cosmetics with various salt concentrations, the complex will have a relatively uniform nano-sized small particle shape and can be dissolved and stably existed without sedimentation. -It is believed that it will be able to facilitate commercialization of products containing HPβCD.

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Measurement Example 4

When the pH of the aqueous solution containing the extract carbohydrate nanocomposite (ST-L10D-HPβCD) was adjusted from 2 to 7, there was no significant change in the particle size, and as the pH decreased, the PDI value exceeded 0.5 from pH 5 However, the absolute value of the zeta potential decreased as the pH decreased, but at pH 3 or higher, except for the case where the pH was very low 2, the zeta potential was increased, but did not exceed 1.0. It has a value of about 7 to 18, and in the case of this value, it can be said that it is stably present in the aqueous solution. (Fig. 4(b)) In addition, no precipitate was observed when left for 12 hours in an aqueous solution of pH 2 to 7 .(Fig. 4(c))

Therefore, in the case of ST-L10D-HPβCD, when used in foods or cosmetics with various pHs, the complex may have a relatively uniform nano-sized small particle shape and stably dissolved and exist without sedimentation. -It is believed that it will be able to facilitate commercialization of products containing HPβCD.

Measurement Example 5

In the case of the DPPH radical scavenging capacity (diphenyl-2-picrylhydrazyl Radical-scavenging capacity) of ST alone, that is, the antioxidant capacity, the radical scavenging capacity increased as the ST concentration increased. On the other hand, in the case of the extract carbohydrate nanocomposite (ST-L10D-HPβCD), it exhibited a high radical scavenging ability even at a small concentration of ST. Compared to the case where giraffe extract (ST) was used alone, it exhibited the same antioxidant ability even in a smaller amount. As a result, it showed superior antioxidant ability than the sum giraffe extract (ST) itself.

Measurement Example 6

The antibacterial ability of the sumirincho extract itself (ST) and the carbohydrate nanocomposite of the sumirincho extract of the present invention (ST-L10D-HPβCD) were tested, and the results are shown in Table 1 below.

MIC: minimum inhibition concentration. 50%DMSO: 50% concentration of dimethyl sulfoxide

According to Table 1 above, in relation to the antibacterial ability of ST-L10D-HPβCD, when the ST-L10D-HPβCD complex is dissolved in 50% DMSO or water, a higher minimum inhibitory concentration ( MIC) is required. However, since the amount of 1,2,4,6-tetra-O-galloyl-β-D-glucose, which is an active ingredient in the extract, which has an antibacterial effect, is present at 0.72% in the mixed solution in which the complex is present, Compared to the case where 2.82% of the active ingredient is present in the present mixed solution, a much smaller amount of the active ingredient is required. Therefore, it can be seen that the ST-L10D-HPβCD complex exhibits a better antibacterial effect than the sumirin herb extract (ST) itself.

As described above, specific parts of the present invention have been described in detail, and for those of ordinary skill in the art, it is obvious that these specific techniques are only preferred embodiments, and the scope of the present invention is not limited thereby. something to do. Therefore, it will be said that the practical scope of the present invention is defined by the appended claims and their equivalents.

Claims (6)

As a carbohydrate nanocomposite from the extract of Sesame chinensis,
The sumirincho extract carbohydrate nanocomposite contains the same weight ratio of 1:1:1, including the extract of the sumirincho, beta-cyclodextrin, and decaglyceryl laurate, and is a dry powder. Sumirincho extract carbohydrate nanocomplex.


delete (a) dissolving a complex combining the extract and decaglyceryl laurate in water;
(b) dissolving β-cycliodextrin in ethanol;
(c) mixing the solution prepared in step (a) and step (b);
(d) removing ethanol from the mixed solution passed through step (c);
(e) freeze-drying from the mixed solution passed through the step (d) to remove water, and in the above steps, extract, beta-cycliodextrin, and decaglyceryl laurate ) Is a method for producing a carbohydrate nanocomposite of claim 1, characterized in that it is included in the same weight ratio of 1:1:1.



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