KR100642939B1 - Bathroom soap comprising plum extracts and extracting method thereof - Google Patents

Abstract

It replaces the chemical antimicrobial agent and provides cosmetic soap containing plum extract which shows antibacterial effect even in small amount against human pathogenic bacteria including skin superficial bacteria such as acne bacteria. The cosmetic soap of the present invention contains 0.006 to 5 parts by weight of natural plum extract, 0.1 to 1 part by weight of licorice extract, 0.05 to 5 parts by weight of vitamin E acetate, and 0.1 to 10 parts by weight of polyethylene glycol. Plum extract is obtained by extracting the hexane fraction of the plum using hexane from the methanol extract of plum, chloroform is added to the lower layer separated from the hexane fraction extract to obtain a chloroform extract, and ethyl acetate is added to the upper layer separated by the chloroform extract. To obtain an ethyl acetate fraction, butanol was mixed in the lower layer separated by ethyl acetate, and the butanol fraction and the water layer separated from the butanol were concentrated under reduced pressure to obtain a water fraction.

Cosmetic soap, plum soap, plum extract, vitamin E acetate, polyethylene glycol

Description

Cosmetic soap containing plum extract and its extraction method {BATHROOM SOAP COMPRISING PLUM EXTRACTS AND EXTRACTING METHOD THEREOF}

The present invention relates to a cosmetic soap containing a natural plum extract, and more particularly to a cosmetic soap containing a plum extract, licorice extract, vitamin E acetate and polyethylene glycol as a main component.

In general, acne is more common in puberty men and women as inflammation of the hair follicles in the face, especially cheeks and forehead. Acne occurs badly when the secretion of hormones such as androgens increases. Androgens cause sebaceous glands to become large, and the sebaceous glands are stimulated by androgens to produce larger sebum. Acne is caused by the clogging of the sebaceous glands out, and sebaceous glands are blocked, sebum accumulates, and swells up and becomes large enough to be identified by the eye, forming a cotton patch. Inside this cotton are grown anaerobic, sebum-loving acne bacteria (Propionibacterium acnes). Inflammation caused by acne bacteria can cause skin damage and cause secondary bacterial infections. In this case, the irritating ingredients of the general soap may further damage the skin by further expanding the initial inflammation and the secondary inflammation.

In the conventional soap ingredients, fatty acid and fatty acid derivatives, plant and animal fats and fats, fatty alcohols, lanolin fluids, and other fat removal agents and various plant extracts have been used. Since the hyperfat remover and the plant extract are dispersed in an aqueous soap solution and adsorbed on the skin, the skin feels good after washing, and there is a cosmetic effect on the skin. The problem of discoloration, discoloration, etc. due to long-term preservation by addition and high solubility is a problem that the physical properties of the soap, such as being quickly consumed and the hardness is receded lower than the level of the general cosmetic soap, and reduces the quality of the product. Therefore, plant extracts that provide antibacterial, anti-aging and moisturizing effects of the skin should be added in small amounts to maintain the properties of the soap and exert its functionality.

In addition, conventionally used cosmetic soap does not have an antimicrobial effect on bacteria causing skin diseases such as acne bacteria, and when used frequently, there is a problem that the skin is dried and roughened by removing sebum components of sebum and softening keratin.

In order to solve this problem, various additives have been presented in the composition of high-grade soap, and a synthetic antibiotic with excellent antimicrobial effect was used to remove pathogenic bacteria such as acne bacteria.

As a synthetic antimicrobial agent, 3,4,4'-trichlorocarbanilide, 4,4'-dichloro-3- (trifluoromethyl) carbanilide, 3,4,5-tribrosalicyla-anilide alone or 0.1-3 weight part can be added by mixing.

However, when the chemical synthetic antimicrobial agent is added, there are many side effects such as generation of resistant bacteria and the texture of the soap is lowered, and the synthetic antimicrobial agent has a problem that is difficult to use because it is an expensive medicine.

The present inventors conducted a study to solve the above problems, it was found that the plum extract extracted from natural plum has a strong antibacterial effect against human pathogenic bacteria including acne bacteria.

Accordingly, it is an object of the present invention to provide a cosmetic soap containing a plum extract that exhibits an antimicrobial effect even in a small amount against human pathogenic bacteria, including superficial bacteria such as acne, in place of conventional chemical antibacterial agents. do.

In order to achieve the above object, the cosmetic soap of the present invention contains 0.006 to 5 parts by weight of natural plum extract, 0.1 to 1 parts by weight of licorice extract, 0.05 to 5 parts by weight of vitamin E acetate and 0.1 to 10 parts by weight of polyethylene glycol.

Preferably, the plum extract may contain 0.006 parts by weight, vitamin E acetate 0.1-3 parts by weight, polyethylene glycol 0.2-5 parts by weight.

In addition, it is preferable to use the ethyl acetate fraction having the best antibacterial effect as the plum extract.

The cosmetic soap of the present invention may further contain conventional fragrances, pigments, dyes, etc., such as soap components and surfactants commonly used in cosmetic soap.

In the present invention, using the hexane from the methanol extract of plum to obtain the hexane fraction extract (KC-1) of the plum, chloroform was added to the lower layer separated from the hexane fraction extract to obtain a chloroform fraction (KC-02), Ethyl acetate was added to the upper layer separated by chloroform fraction to obtain ethyl acetate fraction (KC-03), and butanol was separated into butanol fraction (KC-04) and the butanol separated on the lower layer separated by ethyl acetate. Concentrating the water layer under reduced pressure provides a method for extracting plum extract, comprising the step of obtaining a water fraction (KC-05).

In the present invention, an excellent antibacterial prune extract that can replace the chemical antimicrobial agent was obtained through various extraction methods. In order to obtain such an extract, a fractionation method using an organic solvent and a purification process must be performed, and a material having excellent antimicrobial effect on the extracted and purified material must be secured first.

Prunes contain a small amount of vitamin E, which is excellent for skin aging prevention and regeneration. Amidarin ingredient has a natural bactericidal effect, which is good for skin cleanliness. In addition, the plum oil ingredient forms a breathable film when used on the skin, which has excellent affinity with the skin, excellent skin softening effect, and protects the skin from external stimuli according to the formation of the film.

When the plum extract having various cosmetic effects is mixed in a large amount of soap without refining, problems such as discoloration and odor occur when stored for a long time, so the extract having excellent antibacterial effect should be extracted and purified in small amounts.

On the other hand, in order to produce a functional cosmetic soap that has an excellent antimicrobial effect, the composition ratio of the soap is important. In the present invention, first, substances such as vitamin E acetate and polyethylene glycol are used to increase the functionalities such as protection of the skin and anti-aging.

Vitamin E acetate (tocopheryl acetate) is acetate to stabilize tocopherol, and when used on skin, it synergistically acts on the antioxidant activity of keratin, a constituent protein of skin tissues, and prevents skin oxidation. To prevent the oxidation of unsaturated fats and oils of sebum to inhibit the production of peroxides, thereby alleviating irritation to the skin.

In addition, when the plum extract and vitamin E acetate are added to the cosmetic soap, both components have no problem in the addition of small amounts in the liquid phase, but when the large amount is added for the cosmetic effect of the skin, the hardness of the soap is lowered and it is difficult to manufacture. There is this. Accordingly, in the present invention, when polyethylene glycol is mixed with these components, soap, solubility, hardness, and the like of the soap are improved, foaming ability is increased, and the cosmetic soap which can sufficiently give the effect of prune extract and tocopherol on the skin can be prepared. Could.

The use of such a composition in the soap is excellent in the cosmetic effect on the skin, there is no problem of deterioration due to long-term preservation, and it was possible to prepare a cosmetic soap having the same physical properties as the general cosmetic soap, solubility in water, hardness, etc. .

Referring to the composition ratio of the cosmetic soap of the present invention in more detail as follows.

Cosmetic soap containing the plum extract of the present invention, 0.006 parts by weight of ethyl acetate extract (KC-03) of the excellent antibacterial effect, 0.15 parts by weight of licorice extract, a functional ingredient to improve skin beauty, 0.1 parts by weight of vitamin E acetate And 4.0 parts by weight of polyethylene glycol, wherein 10 parts by weight of sodium coconut patioside and 10 parts by weight of sodium coconut are mixed with 25 parts by weight of sodium cocosil isethionate as a surfactant and 16 parts by weight of sodium cocosulphate. do.

KC-03, a functional antimicrobial component prepared by drying and solvent extraction in plums, has a pH of about 4-5 in an aqueous solution of 0.006 parts by weight. Vitamin E acetate may be used by acetate-forming natural vitamin E, or synthetic vitamin E may be used. In addition, polyethylene glycol may be prepared by polymerizing ethylene oxide.

The cosmetic soap of the present invention may further contain any component conventionally used for cosmetic soap. Typically, the cosmetic soap contains about 8 to 20 parts by weight of water, and 0.2 parts by weight or less of EDTA, which is a conventional heavy metal ion disintegrant, is added. Ordinary flavors, pigments, dyes and the like can also be added to about 5 parts by weight or less.

Soap is prepared by putting a make-up soap containing 8 to 20 parts by weight of moisture in a constant mixer, adding flavorings, dyes, and other additives, which are common additives, and then mixing with a mixer, extracts of plum, licorice, vitamin E acetate and After the polyethylene glycol is mixed, it is produced by extrusion, cutting and molding in a conventional manner.

Hereinafter, the process of preparing the plum soap of the present invention will be described in more detail.

1. Extraction of antibacterial ingredients of plums.

Plum fruit is used to extract functional antimicrobial ingredients from the plums. Plum extract was carried out in the following manner. Whole fresh plums are enucleated and then dried in a hot air dryer at 60 ° C. until the level is completely removed. The dried plums are crushed in a grinder, then placed in an extraction container and chilled by adding 3 times the amount of methanol. The cold soak time is conducted over night and lasts until sufficient extraction time has passed.

Methanol extract of prunes is added 1 v / v to the separatory funnel and the ratio of 10 v / v of hexane and 9 v / v of water (plum methanol extract: water: hexane = 1: 9: 10) is added. Shake each solvent repeatedly to mix well, then stagnate to separate the hexane and water layers. The water layer formed on the lower layer is well separated and stored in another separatory funnel, and the organic solvent hexane is added in the same manner and extracted three times. The separated hexane layer is concentrated under reduced pressure at 80 ℃ to obtain a hexane fraction extract of plum (KC-1).

The lower layer separated from the hexane layer was extracted by adding chloroform 1: 1 v / v, and then extracted three times, thereby obtaining KC-02 (chloroform fraction) by concentration under reduced pressure at 80 ° C.

The ethyl acetate was added 1: 1 v / v to the upper layer separated by a chloroform layer, mixed well, extracted three times, and concentrated under reduced pressure at 80 ° C. to obtain KC-03 (ethyl acetate fraction).

In the lower layer separated by ethyl acetate, butanol was added in an amount of 1: 1, mixed and extracted three times. The separated upper layer was concentrated under reduced pressure at 80 ° C. as a butanol layer to obtain KC-04 component.

Finally, the water layer separated by butanol was concentrated under reduced pressure at 80 ℃ to obtain a KC-05 component. In addition, the crude crude extract extracted with methanol is separated at 80 ° C. to obtain a KC-06 component, which is used as a functional ingredient of plum soap.

2. Screening of Antimicrobial Substances from Plums

1) Minimum development concentration measurement

Fleisch extract broth and Agar were used as strain culture medium to measure the minimum inhibitory concentration. The composition was 1% of Beef extract, 1% of Peptone, Sodium chloride 0.3%, Na2HPO4 12 H2O 0.2%, agar 2%, pH 7.4-7.5. In addition, as a medium for measuring the antimicrobial activity, Muller Hinton (Muller Hinton) liquid medium and solid medium were used by Diffco Inc., strains difficult to culture were cultured by adding a certain amount of immune serum to the medium. The test strains were 20 strains for testing the minimum inhibitory concentrations, which were distributed by Hoechst (Germany). The quality control of the strains was performed by inoculating the test bacteria on a plate containing standard antibiotics each time the minimum inhibitory concentration was measured. The degree of control of the strain was compared with the minimum inhibitory concentration of

Gentamicin commercially available from Sigma was used as a standard antimicrobial agent for positive control.

The minimum inhibitory concentration (MIC) was measured by the following method. In a series of test tubes, 0.1N NaOH aqueous solution was added 1.7 ml into the first test tube, and the remaining test tubes were dispensed by numbering 1.5 ml each, and then 0.3 ml standard antibiotic solution and test substance were added to the first test tube. After mixing, 1.5 ml was taken, transferred to a second test tube, mixed well, and then transferred back to a third test tube. This operation was performed up to the seventeenth test tube to make the dilution series twice. Thereafter, the sterilized Muller-Hilton agar medium was cooled to 45 ° C., 13.5 ml of each of the test tubes diluted in multiples of 2, poured into a petri dish, and mixed well to prepare 17 flat media. The concentration of the inoculating bacteria was adjusted by diluting the test bacteria precultured for about 18 hours in the fly's extraction liquid medium or the fly's extraction liquid medium with serum. Each test organism was placed in an inoculation tray and inoculated at 104 CFU / spot on a plate containing antimicrobial agent using an automatic inoculator (MCT Medical Co., Model No. 2, USA) and incubated at 37 ° C. for 18 hours. Afterwards, the plated inoculated by diluting step by step 2 times and lined up with the naked eye to determine the concentration of the antimicrobial agent inhibited growth to the minimum growth inhibitory concentration (MIC). At this time, Streptococcus mutans KCTC3065 and Propionibacterium acnes (hereinafter referred to as 'P.') acnes KCTC 3314 strains were anaerobicly cultured, and Propionibacterium acne strains were EM Medium was used.

2) Antimicrobial Effect of Plum Fractions on Pathogenic Bacteria

① Antibacterial effect against Streptococcus group

Plum extract, separated from natural dried plums, KC-1 (hexane fractionation layer), KC-2 (chloroform fractionation layer), KC-3 (ethyl acetate fractionation layer), KC-4 (butanol fractionation layer), KC- 5 (water fractionation layer) and KC-06 (hot water extract) were used to investigate the antimicrobial activity against various human pathogens.

These fractions showed a minimum inhibition concentration (MIC) against Staphylococcus aureus superior to other human pathogens as shown in Table 1. All fractions showed 2.0 mg / ml antimicrobial activity against Streptococcus pyogens A77, whereas KC-3 fractions showed excellent antimicrobial effect of 0.006 mg / ml. It was.

The synthetic antibiotic gentamycin used as a control had a MIC concentration of 0.2 µg / ml. Among the prune extracts, the lowest inhibitory concentration for Streptococcus piyogens was PE-3, 3.2 mg / ml, which was the lowest MIC among the 20 strains tested. S. pyogens are bacteria that mainly cause purulent inflammation such as furuncle, carbuncle, cellulitis, sole, tonsillitis, sinusitis, pharyngitis, laryngitis, otitis media and osteomyelitis, including skin diseases. Therefore, the cosmetic soap containing the plum extract of the present invention has a great effect in suppressing pathogenic bacteria of human skin causing skin diseases.

Table 1. Minimum Inhibitory Concentrations of Plum Extracts on the Growth of Streptococcus

     Bacterial strains             Minimum inhibitory concentration (MIC, mg / ml) GEN (μg / ml) KC-01 KC-02 KC-03 KC-04 KC-05 KC-06 S. Piyozens A308 2.0 2.0 0.006 > 2.0 2.0 > 2.0 0.2 S. Piyozens A77 2.0 2.0 0.006 > 2.0 > 2.0 2.0 0.05 S. faecium MD8b > 4.0 > 2.0 > 0.011 > 2.0 > 2.0 > 2.0 6.25     Mean ± S.D 2.7 ± 1.2 2.0 ± 0 0.007 ± 0.002 2.0 ± 0 2.0 ± 7.2 2.0 ± 0 2.1 ± 3.5

GEN: Gentamicin (positive control)

② Antibacterial effect against Staphylococcus group

Table 2. Minimum Inhibitory Concentrations of Plum Extracts on the Growth of Staphylococcus Aureus (MIC)

    Bacterial strains             Minimum inhibitory concentration (MIC GEN (μg / ml) KC-01 KC-02 KC-03 KC-04 KC-05 KC-06 S. aureus SG511 > 4.0 > 2.0 0.006 > 2.0 > 2.0 > 2.0 0.1 S. aureus 285 > 4.0 > 2.0 0.006 > 2.0 > 2.0 > 2.0 0.05 S. aureus 503 4.0 2.0 0.006 > 2.0 > 2.0 > 2.0 0.4    Mean ± S.D 4.0 ± 0 2.0 ± 0 0.006 ± 0 2.0 ± 0 2.0 ± 0 2.0 ± 0 0.18 ± 0.2

GEN: Gentamicin (positive control)

Table 2 shows the antimicrobial effects of four strains of Staphylococcus aureus using four plum fractions. Like the antimicrobial activity of prune extract against Streptococcus, the KC-03 fraction showed excellent antimicrobial activity at a MIC concentration of 0.006 mg / ml against Staphylococcus aureus. As a result, it can be seen that the plum KC-03 fraction is aerobic and shows excellent antimicrobial activity against staphylococcus aureus causing epidermal purulent inflammation and can be used as a raw material for cosmetics and soap.

③ Antibacterial effect on E. coli (Eschericheia (hereinafter 'E.') coli) group

Table 3. Minimum Inhibitory Concentrations of Plum Extracts on the Growth of Escherichia Coli Group

    Bacterial strains                Minimum inhibitory concentration (MIC GEN (μg / ml) KC-01 KC-02 KC-03 KC-04 KC-05 KC-06 E. coli O 78 4.0 2.0 0.006 > 2.0 > 2.0 > 2.0 0.4 E. coli DC 0 > 4.0 > 2.0 > 0.011 > 2.0 > 2.0 > 2.0 0.4 E. coli DC 2 4.0 2.0 0.006 > 2.0 > 2.0 > 2.0 0.4 E. Coli TEM > 4.0 > 2.0 > 0.011 > 2.0 > 2.0 > 2.0 0.4 E. Coli 1507E > 4.0 > 2.0 > 0.011 > 2.0 > 2.0 > 2.0 0.4   Mean ± S.D 4.0 ± 0 2.0 ± 0 0.009 ± 0.003 2.0 ± 0 2.0 ± 0 2.0 ± 0 0.4 ± 0

GEN: Gentamicin (positive control)

Table 6 shows the results of antimicrobial effects by measuring the MIC concentrations for five strains of E. coli using six plum fractions. Each of the plum extracts showed the same MIC for the five strains of E. coli, but the KC-03 showed excellent average 0.009 ± 0.003mg / ml. In other words, KC-03 showed 0.006 mg / ml for E. coli O 78 and 0.006 mg / ml for E. coli DC 2, and MIC concentrations> 0.011 mg / ml for other strains.

④ The antimicrobial effect on Pseudomonas (P.'laram) aeruginosa group

Table 4. Minimum Inhibitory Concentration of Extracts of Plum Extracts on Growth of Pseudomonas aeruginosa Population

    Bacterial strains               Minimum inhibitory concentration (MIC GEN (μg / ml) KC-01 KC-02 KC-03 KC-04 KC-05 KC-06 P. Eruzinosa 9027 4.0 2.0 > 0.011 > 2.0 > 2.0 > 2.0 0.79 P. aeruginosa 1592E 4.0 2.0 > 0.011 > 2.0 > 2.0 > 2.0 0.79 P. Eruzinosa 1771 4.0 2.0 0.006 > 2.0 > 2.0 > 2.0 12.5 P. aeruginosa 1771M 4.0 2.0 0.006 > 2.0 > 2.0 > 2.0 0.4 Mean ± S.D 4.0 ± 0 2.0 ± 0 0.008 ± 0.003 2.0 ± 0 2.0 ± 0 2.0 ± 0 3.62 ± 5.9

GEN: Gentamicin (positive control)

The antimicrobial effect was confirmed by measuring the MIC concentrations of four P. aeruginosa strains using six prune extracts, and the results are shown in Table 4. Each of the prune extracts showed mostly similar MIC for the four Pseudomonas aeruginosa strains, but KC-03 showed a minimum inhibitory concentration of 0.008 ± 0.003 mg / mL on average for all tested Pseudomonas aeruginosa strains. It can be seen that the KC-03 component exhibits excellent antibacterial activity against Pseudomonas aeruginosa which is a cause of wound infection and skin infection.

⑤ Antibacterial effect against Salmonella, Klebsiella, Enterobacter (Salmonella, Klebsiella, Enterobacter)

Table 5. Minimum Inhibitory Concentrations (MIC) of Plum Extracts on Growth of Salmonella, Krebsiella and Enterobacter Bacillus Groups

    Bacterial strains          Minimum inhibitory concentration (MIC GEN (μg / ml) KC-01 KC-02 KC-03 KC-04 KC-05 KC-06 Salmonella Typhimurium > 4.0 > 2.0 > 0.011 > 2.0 > 2.0 > 2.0 0.4 Krebsciella oxytoca 1082E > 4.0 2.0 > 0.011 > 2.0 > 2.0 > 2.0 0.4 Krebsciela Eruzinosa 1522E > 4.0 > 2.0 > 0.011 > 2.0 > 2.0 > 2.0 0.4 Enterobacter cloacae P99 4.0 2.0 > 0.011 > 2.0 > 2.0 > 2.0 0.2 Enterobacter cloacae 1321E 4.0 2.0 > 0.011 > 2.0 > 2.0 > 2.0 0.2    Mean ± S.D 4.0 ± 0 2.0 ± 0 0.008 ± 0.003 2.0 ± 0 2.0 ± 0 2.0 ± 0 0.32 ± 0.1

GEN: Gentamicin (positive control)

Table 6 shows the results of measuring the MIC concentrations of Salmonella typhimurium 1 strain, Klebsiella eruginosa strain 2, and Enterobacter cloacae strain 2 using six prune extracts. KC-01, a hexane extract of plum, showed the same MIC at 4 mg / ml or higher, such as Salmonella, Klebsiella, Enterobacter, etc. The effect was 2.0 mg / ml with low effect. In addition, the ethyl acetate fraction extract KC-03 showed the same 0.011 mg / ㎖ against the strain showed an excellent antimicrobial effect, KC-04, KC-05 and KC-06 showed the same MIC of 2.0 mg / ㎖ It was. From these results, it can be seen that the antibacterial effect on the enterobacterial group according to the extraction conditions.

⑥ Antibacterial effect against S. mutans

Table 6. Minimum Inhibitory Concentrations of Plum Extracts on the Growth of Streptococcus Mutans (MIC)

      Bacterial strains             Minimum inhibitory concentration (MIC GEN (μg / ml) KC-01 KC-02 KC-03 KC-04 KC-05 KC-06 S. Mutans KCTC3065 > 4.0 > 2.0 0.7 > 2.0 > 2.0 > 2.00 0.4

GEN: Gentamicin (positive control)

Table 6 shows the results of measuring the MIC concentration of S. mutans KCTC3065 strain 1 strain using six plum extract extracts. Plum extract of S. mutans, the most common causative agent of dental caries, showed better antibacterial effect than enterobacteriaceae. In particular, KC-03 showed a minimum inhibitory concentration of 0.7 mg / ml.

⑦ Antibacterial effect on propionibacterium acnes KCTC 3314

Table 7. Minimum Inhibitory Concentrations of Plum Extracts on Growth of P. Acnes (MIC)

     Bacterial strains             Minimum inhibitory concentration (MIC GEN (μg / ml) KC-01 KC-02 KC-03 KC-04 KC-05 KC-06 P. Aknes KCTC3314 2.0 > 2.0 0.006 > 2.0 > 2.0 > 2.00 0.4

GEN: Gentamicin (positive control)

Table 7 shows the results of measuring the MIC concentrations for P. Acnes KCTC3314 using six plum extract extracts. Plum extract showed excellent antibacterial effect against P. acnes, the causative agent of acne, and it can be seen that it can be used as an active ingredient of soap.

3. Characteristics of KC-03 with excellent antibacterial effect

Among the organic solvent extracts of plum, KC-03 has the best antibacterial effect. It is pH 3.5 ~ 4.5 at 0.006%, which is water soluble. . In addition, KC-03 is stable at a temperature of 100 ℃, it is stable against factors such as discoloration, light, temperature. Plum, in particular, is an edible fruit, which is harmless to the human body, so KC-03 is also considered to be harmless.

4. Composition of plum soap

Plum soap of the present invention is developed as a functional beauty soap, is a multifunctional beauty soap made of pure vegetable. A survey of the sensory tests on soaps indicated that it is good for skin care as well as for improving acne.

PH of plum soap; In the manufacture of neutral soaps, sodium hydroxide is necessary and is decomposed into Na and OH in the process of non-crosslinking, which is divided into fatty acid salts, glycerin and water. NaOH added during the saponification reaction should be completely decomposed during the completion of the soap so that almost no NaOH is present.

1) Composition of neutral plum soap

 Table 8. Composition of neutron plum soap (contents are by weight)

                  ingredient  Example 1  Comparative Example 1  Comparative Example 2 Sodium cocoyl isethionate  25.0  25.0  25.0 Sodium cocosulfate 16.0 16.0 16.0 Coconut fatty acid 10.0 10.0 10.0 Sodium cocoate 10.0 10.0 10.0 Cetaryl alchol 10.0 10.0 10.0 Sodium laurate 5.0 5.0 5.0 Cocoyl Glutamic Acid 5.0 5.0 5.0 Polyethylene Glycol (PEG-400) 4.0 4.0 4.0 Sodium steatrate 4.0 4.0 4.0 Coconut Fatty Acid Ethanolamide 3.0 3.0 3.0 Deionized purified water 2.7494 2.7494 2.7494 Alkyl betain 2.0 2.0 2.0 glycerin 2.0 2.0 2.0 Incense 0.8 0.8 0.8 Polyethylene glycol-75 (PEG-75) lanolin 0.1 0.1 0.1 Sodium EDTA 0.1 0.1 0.1 Vitamin E Acetate 0.1 0.1 0.1 Glycyrrhiza extract 0.15 0.15 - Plum Extract-KC 03 Ingredients 0.0006 - - pH 7.4 7.5 7.5

5. Test Evaluation of Plum Soap

The test evaluation of the plum soap obtained by this invention is demonstrated in detail as an Example and a comparative example. Here, the Example is a soap containing a plum extract of the present invention, the comparative example is for comparison as a soap does not contain a plum extract.

Using soap prepared by the composition of Example 1, Comparative Example 1 and Comparative Example 2, the acne improvement effect, the feeling of use, stability at 40 ℃, hardness test for college students having acne on the skin Evaluation was carried out, and the results are shown in Tables 9 to 12.

1) Feeling comparison test

A test was conducted on soaps of Example 1 and Comparative Examples 1 and 2 with 90 acne male and female college students. The method of use was used for 30 days as in the case of using general cosmetic soap, and the result was evaluated through a questionnaire after 30 days.

In the evaluation of the test, the moisture of the skin was completely removed, and the usability to the skin was compared and the average score of 90 people was obtained. The evaluation score is 5 points for "skin is very good" than normal makeup soap, 4 points for "skin is better" than general makeup soap, 3 points for "same" with normal makeup soap, and normal makeup soap Two points of "skin slightly rougher" and one point of "rougher skin than normal toilet soap" were added, and the percentage of respondents for each item was calculated.

Table 9. Feeling test of ordinary soap and plum soap

            Evaluation item   Example 1   Comparative Example 1   Comparative Example 2 Skin becomes very rough 3.3 - 3.3 The skin is slightly roughened. 13.3 16.6 16.7 is average 23.3 30.0 30.0 Skin got better 40.0 36.7 33.3 My skin is very good 20.0 16.7 16.7 Sum 100 100 100

As can be seen in Table 9, among the people who used the soaps of Comparative Examples 1 and 2, the positive responders to "good skin" and "very good" were 53.4% and 50%, respectively. In the case of the soap of 1, the response was 60%, it can be seen that showed a better feeling than ordinary soap. Alkali soaps are irritating, whereas prune soaps are neutral soaps, and they contain ingredients such as coconut fatty acid diethanol amide, cocosil glutamic acid, and alkyl betaine, and licorice extract alleviates skin irritation. This is because polyethylene glycol-75 lanolin, a ning agent, is contained.

2) Comparison test of acne improvement effect

Table 10. Acne improvement effect test

Evaluation item Example 1 Comparative Example 1 Comparative Example 2 Acne was very bad - 3.3 6.7 Acne slightly worsened 13.3 16.6 20.0 No change 23.3 30.0 33.3 Improves acne 43.3 33.3 26.7 Acne is very effective 20.0 16.7 13.3 Sum 100 100 100

As can be seen in Table 10, among the people who used the soaps of Comparative Examples 1 and 2, 50.0% of the respondents who answered positively to "the effect of improving the acne" and "the improvement of the acne very well" were 50.0% and Although it was 40%, the soap of Example 1 responded to 63.3% and had an effect of alleviating acne than ordinary soap. In particular, Example 1 containing the plum extract showed a greater improvement of 10-20% or more than the comparative example without the plum extract. This is believed to be due to the direct antibacterial effect and hypoallergenicity of the neutral soap to alleviate the acne inflammatory response.

Antibacterial Effects of Plum Soap Containing KC-03 on Acne Bacteria

3) Stability test at 40 ° C

   After putting the cosmetic soaps prepared in the compositions of Example 1 and Comparative Examples 1 and 2 in a 40 ° C. thermostatic chamber and storing them for one month, it was determined whether the cosmetic soap had discoloration and odor. The soap containing the plum extract of the present invention did not change at all, and the soap of the comparative example showed some discoloration and odor. The results are shown in Table 11.

Table 11. Safety Tests

         exam Example 1 Comparative Example 1 Comparative Example 2 Stability at 40 ℃ No change at all Slight change Slight change

4) hardness test of plum soap

The hardness test was tested for the cosmetic soaps prepared in the compositions of Example 1 and Comparative Examples 1 and 2. Rheo meter was used for the hardness measurement, and a 20 × 20 × 40 mm soap sample was carried out under the conditions of adapter No. 3, speed 6 cm / min, measuring position 1 cm, and measuring temperature of 30 ° C.

Table 12 Hardness Test

      exam       Example 1       Comparative Example 1       Comparative Example 2 Hardness         130         130         130

According to the results of Table 12, the soap of Example 1 and Comparative Examples 1 and 2 did not change the hardness even if the plum extract is included, the feeling of use for the skin is lower than the hardness of 200 ~ 300 that the general soap has good.

As described above, the cosmetic soap containing the plum extract obtained in the present invention has superior antibacterial effect, cosmetic beauty effect, stability and physical properties as compared to cosmetic soap containing a general cosmetic soap and hyperfat or plant extract. Excellent solubility and hardness

In addition, by separating the components excellent in antimicrobial activity from prunes and use in the cosmetic soap can remove the pathogenic bacteria causing various skin diseases, so as to improve the functionality of the soap as well as to use the prunes.

In particular, the active ingredient of the plum fruit is not only an antibacterial effect, but also prevents skin aging and abundance, and thus has a skin regeneration effect, and may be used in various ways as a raw material of functional cosmetics in the future.

Claims (6)

Antibacterial soap for acne improvement containing 0.006 to 5 parts by weight of ethyl acetate extract of plum. The antibacterial soap according to claim 1, further comprising 0.1 to 1 parts by weight of licorice extract, 0.05 to 5 parts by weight of vitamin E acetate, and 0.1 to 10 parts by weight of polyethylene glycol. The method according to claim 1 or 2, characterized in that it contains 0.006 parts by weight of ethyl acetate extract of prunes, 0.1 to 3 parts by weight of vitamin E acetate, 0.2 to 5 parts by weight of polyethylene glycol, surfactants, fragrances, pigments and dyes. Antibacterial soap. delete delete From the methanol extract of plum, hexane fraction extract (KC-1) was obtained using hexane, chloroform was added to the lower layer separated from the hexane fraction extract to obtain chloroform fraction (KC-02), and the chloroform fraction was separated. Ethyl acetate was added to the upper layer to obtain an ethyl acetate fraction (KC-03), butanol was mixed into the lower layer separated by the ethyl acetate, and the butanol fraction (KC-04) and the water layer separated from the butanol were concentrated under reduced pressure. Extraction method of plum extract, comprising the step of obtaining water fraction (KC-05).