KR100441131B1 - Cosmetic compositons for acne skin containing natural fragrant oil - Google Patents

Abstract

The present invention relates to an acne cosmetic composition containing natural ointment oak leaves, which contains 0.0001 to 5% by weight of the oak oak leaf extract, based on the total weight of the composition, and oak leaf extract It is a cosmetic composition for improving acne skin having excellent acne prevention and alleviating ability by inhibiting the growth of propionibacterium acne and inhibiting the activity of 5α-reductase by the fragrance oil component in.

Description

Acne cosmetic composition containing natural balm {COSMETIC COMPOSITONS FOR ACNE SKIN CONTAINING NATURAL FRAGRANT OIL}

The present invention relates to an acne cosmetic compositions containing the natural enjoyment (香油), more specifically, the causative agent of acne propionic sludge 5α- Lee involved in sebum hypersecretion of growth inhibitory ability and acne patients tumefaciens acne (Propionibacterium acnes) The present invention relates to a cosmetic composition having an anti-acne prevention and alleviating effect, which contains a natural antibacterial perfume oil extracted from the leaves of Thuja koraiensis nakai , which has an excellent inhibitory activity of ductase .

Acne is known to be caused by a combination of various causes. In general, acne has increased secretion of sebaceous glands due to increased male hormone function in puberty, and epithelial cells of hair follicles cause abnormal keratosis and blockage of hair follicles. It is recognized that the propensium bacterium Propionibacterium acne actively proliferates to produce free fatty acid, which stimulates hair follicles and causes acne inflammation. This progression to acne is exacerbated by sebum hypersecretion associated with 5α-reductase.

Conventionally, as a method for treating or alleviating acne, a method using a female hormone such as estrogen, a male hormone inhibitor, or an antibacterial agent has been used to suppress sebum hypersecretion. In recent years, a method of inhibiting sebum hypersecretion by using a 5α-reductase inhibitor has been proposed.

However, the method of using female hormones has a high possibility of skin inflammation as a side effect during the treatment of acne, and there are many reports of side effects caused by long-term administration of female hormones. However, only a small amount of prescription is carefully used under the observation of a doctor.

In addition, typical antibacterial agents for acne bacteria include resorcinol, sulfur and benzoyl peroxide, tetracycline, erythromycin, or methacycline, but the use of such antimicrobials has some effect on acne treatment. However, there are still various problems in terms of occurrence of antibiotic-specific side effects. For example, retinoic acid and benzoyl peroxide, which are antimicrobial agents, are highly susceptible to skin irritation and contact dermatitis, and antibiotics including tetracycline have been reported to have side effects due to the emergence of resistant bacteria and / or photosensitive action.

In addition, to solve this problem, some cosmetic compositions for improving acne skin containing natural fragrance substances such as tea tree oil or rosemary oil have been proposed, but none of them have satisfactory acne improvement effects. In particular, there is a problem that does not give a good preference to consumers because it has an overly irritating smell in terms of odor.

The present inventors measured the antimicrobial effect against acne bacteria and the inhibitory effect of 5-reductase on the extracts of numerous domestic native aromatic plants with excellent odor to solve the above problems. Natural fragrance oils extracted from the leaves of the cypress have been found to have excellent effects in both of the aspects while the flavor preference is excellent and repeated research has led to the completion of the present invention.

Accordingly, it is an object of the present invention to provide an acne cosmetic composition containing a natural perfume oil as a cosmetic composition for improving acne skin, which contains a natural perfume oil component extracted from the leaves of the eyelids.

Since the cosmetic composition according to the present invention for embodying the object of the present invention contains an eye-opening leaf extract natural perfume oil component having excellent antibacterial activity and excellent 5α-reductase activity inhibitory activity against acne bacteria, It shows excellent acne prevention and alleviating efficacy and is useful as a cosmetic composition for improving acne skin.

Hereinafter, the present invention will be described in more detail.

The natural perfume oil-containing acne cosmetic composition according to the present invention contains a natural perfume oil component extracted from the leaves of the eye tree.

Thuja koraiensis nakai is a native plant native to Korea's Gyeongbuk, Gyeonggi, Gangwon, Pyeongnam and North and South Hambuk provinces. It is an evergreen tree that grows up to 10-20m in height and 20-30cm in diameter. The young branches are smooth and glossy. The leaf is dull, the center is rhomboid, the side is elliptical triangle, the surface is green and the back is yellowish green with two white pores, and the fragrance is very strong and good. Snowy specie is more like a white leaf on the back of a leaf than a cypress, and has seeds with wings and fragrance. It has been described that folk medicine and oriental medicine have been used as a hemostatic agent for treatment of excessive menstruation, postpartum bleeding and mucosal inferior leiomyoma, and also as athlete's foot medicine. Ingredients and Use, "Ilwolgak, 1991).

The natural fragrance oil component derived from the oak leaf used in the present invention may be extracted by various methods such as steam distillation which is generally widely used for oil extraction of a fragrance oil or direct extraction using a polar solvent.

The above steam distillation can be carried out by the method as described, for example, in fragrance chemistry, Japan, Suitability Day, 1983, September, 16, first edition. Specifically, for example, the natural ointment used in the present invention by crushing the oak leaf with a crusher, and then put it in a distillation tank and blows water vapor from below to cool and separate the perfumed ingredient boiled with the water vapor. The component can be obtained easily.

The direct extraction method using the polar solvent described above uses a method of dipping or warming the oak leaf, using a solvent such as methanol, ethanol, acetone, or any mixed solvent thereof. In the present invention, there is no particular limitation on the extraction of the perfume oil component described above. In detail with respect to the above-mentioned direct extraction method, the dry leaves of snow-covered cedars are used in the present invention by immersion extraction for 1 to 14 days in a 5 to 10-fold amount of the solvent described above, or warm extraction for 1 to 5 hours. Snowy cedar leaf extract can be obtained.

When the cosmetic composition for improving acne skin according to the present invention is formulated as an external preparation for skin, the content of the above-mentioned oak cedar leaf balm extract is not limited in the present invention, but 0.0001-5% based on the total weight of the composition, Preferably it is 0.001-1% of range. If the content of the oak leaf foliar oil extract exceeds 5% by weight, it is not recognized that the antibacterial effect against acne bacteria is increased due to the increase of the content, but there is a possibility that the color change and odor due to the change over time may be intense. Not desirable On the other hand, if the content of the oak leaf foliar oil extract is less than 0.0001% by weight, the antibacterial effect and 5α-reductase activity inhibitory ability may be insufficient, which is also undesirable.

The natural fragrance-containing acne cosmetic composition as the cosmetic composition for improving acne skin of the present invention comprising the aforementioned oak leaf extract has excellent antibacterial activity and excellent 5α-reducta against acne bacteria possessed by the oak leaf extract Cosmetic ingredients generally used in external preparations for skin, such as aqueous components, powder components, oils, surfactants, moisturizers, thickeners, preservatives, antioxidants, etc. It can be blended with fragrances, pigments, etc., and it is known and used in various cosmetic formulations such as skin lotions, flexible lotions, astringent lotions, creams, lotions, latex foundations, packs, face washes, and functional soaps. Of course it can be formulated accordingly.

As described above, the functional cosmetic composition for preventing and alleviating acne, which is formulated according to the present invention, can be used alone, but it can be used as a conventional acne treatment agent if necessary within the range not lowering the effect of the functional cosmetic composition of the present invention. Combination with various types of antibacterial agents such as benzoyl peroxide, tetracycline, or erythromycin, anti-androgens, hormones such as steroids, vitamin A derivatives such as retinoic acid, or the above antibacterial agents or physiology It can also be used in combination with cocktail therapy in combination with an active agent, which is expected to be synergistic.

On the other hand, the eye side of the leaf using the extract in the present invention is a material that has been used in herbal medicine for a long time, it has been clinically confirmed that there is safety for the human body even when ingested in the body.

(Example)

Hereinafter, the present invention will be illustrated through Examples, Test Examples, Formulation Examples, etc., but it should be noted that this is not intended to limit the present invention.

Example 1 Extraction of Natural Flavored Eyewood Leaf

Extraction was carried out according to the steam distillation method (Fragrance Chemistry, Japan, Jopseong Wonil, published 1983.9.16, first edition), which is the most widely used fragrance oil, and 1 kg of the mouth of Thuja koraiensis nakai tree Finely crushed, and then put into a distillation tank, forcibly blow water vapor from the bottom to cool and separate the fragrance component of the snow-covered cedar leaf, which boils with the water vapor, Got it.

Test Example 1: Antimicrobial Activity of Natural Perfume Extract

The antibacterial activity of the oak leaf extract obtained in Example 1 was measured by in vitro dilution. After diluting each of the above-mentioned dilutions with various concentrations, the media of the snow-covered cedar leaf oil of Example 1 were used as antibacterial samples, followed by heating and sterilization of Muller Hinton Broth.

5㎖ injected by each of the medium manufactured above in a test tube in a test tube, and the test bacteria (Propionibacterium sludge tumefaciens acne, Propionibacterium acnes KCTC 3314), and then the inoculation, the anaerobic incubated with gently shaken in an incubator maintained at 37 ℃ It was.

After 60 hours of cultivation, the degree of proliferation (turbidity) of the acne-inducing bacteria was compared with a standard curve, and the minimum amount of the above-mentioned natural perfume oil in which the above-mentioned bacteria were inhibited from growth was minimized. (Minimum Inhibitiry Concentration: MIC) was used to determine the antimicrobial activity of the oak leaf extract. The results are shown in Table 1 below.

In addition, as a comparison, MIC was measured by the same operation using benzoyl peroxide, which is widely used as a conventional antimicrobial agent against the acne-inducing bacteria, instead of the oak leaf extract. The results are also shown in Table 1 below.

Antimicrobial Activity of P.acnes from the Extracts of Permanent Oil Extracts sample P.acnes KCTC 3314 (MIC: ppm) Natural balm extract derived from snowy cedar leaf 35 ppm Benzoyl peroxide 1000 ppm

As clearly recognized from Table 1 above, the oak cedar leaf balm extract showed excellent antimicrobial activity compared to benzoyl peroxide, which is widely used as an active ingredient in conventional acne treatments.

Experimental Example 2 5α-reductase Inhibitory Activity of the Extract of Natural Perfume of the Eye of Siberian Fructus

The 5α-reductase inhibitory activity of the oak leaf extract was measured by the following method.

First, partially purified 5α-reductase derived from 8-9 week old rat liver (adult, Sprague-Dawley, male rats) was extracted. Subsequently, a predetermined amount of protein suspension was prepared by protein quantification, and an enzyme reaction solution was prepared by a general method. 5α- reductase reaction substrate as the radioactive label in the testosterone dehydratase ₍₃ H- testosterone), the enzyme reaction using solution: protein suspension: Eye platycladus leaves extract = 7: 2: after reaction at a constant temperature bath 2 minutes with a ratio of 1: 1 reaction After evaporation of the solvent, the residue was dissolved in the developing solvent, thin layer chromatography, and the amount of testosterone developed and the amount of dihydrotestosterone were quantitatively analyzed using a densitometer. The conversion rate of testosterone to dihydrotestosterone by 5α-reductase was calculated and the result was compared with the conversion rate when no control group (eyewood leaf perfume oil extract) was added. It evaluated as inhibitory activity (inhibition rate).

The inhibition rate evaluation method is as follows.

% Inhibition = 100 (A-B / A)

In the inhibition rate, A is the conversion rate of testosterone to dihydrotestosterone when no oak leaf extract is added, and B is the conversion rate of testosterone to dihydrotestosterone when the oak leaf extract is added.

The inhibition rate results are shown in Table 2 below.

Concentration of Natural Flavored Oil Extract of Snow Siberian Foliage (%) 0.1 0.01 0.001 0.0001 % Inhibition 100.0 95.0 76.2 38.3

From the results of Table 2 above, it can be confirmed that the 5α-reductase inhibitory activity of the eye extract of natural cedar leaves.

Examples 2-4 and Comparative Examples 1-3:

Skin lotion as a conventional skin lotion containing acrylonitrile natural balm extract obtained in Example 1, and as a cosmetic composition containing tea tree oil or clover leaf oil as a control, instead of the oak leaf natural balm extract The cosmetic compositions were prepared in Examples 1 to 3 and Comparative Examples 1 to 3, respectively, according to a conventional method, and the compositions thereof are shown in Table 3 below.

ingredient Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 1,3-butylene glycol 3.0 3.0 3.0 3.0 3.0 3.0 ethanol 5.0 5.0 5.0 5.0 5.0 5.0 Polyoxyethylene nonylphenyl ether 0.4 0.4 0.4 0.4 0.4 0.4 Oak leaf natural balm oil 0.1 1.0 2.0 - - - Tea tree oil - - - 0.1 1.0 - Cloverleaf oil - - - - - 2.0 Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount system 100 100 100 100 100 100

Test Example 3: Inhibition of sebum secretion of the skin lotion containing the natural extract of oak leaf

In order to evaluate the sebum secretion inhibitory ability of the natural ointment of the eye prunus leaf contained as an active ingredient in the skin lotion as an acne cosmetic composition according to the present invention, five men and women with sebaceous polysecretory face were tested as follows. .

1) After washing every evening, the skin lotion of Example 2 was applied to the face for a month.

2) Sebum measurement was stabilized in a constant temperature and humidity room for 30 minutes after washing the face, and the amount of sebum on the forehead and cheeks was measured using a sebumeter.

The amount of sebum measured before applying the lotion of the oak leaf natural natural oil extract was set to the initial value. The amount of sebum was measured once a week for 4 weeks, while continuously using the skin lotion of Example 2 containing the oak leaf natural extract.

3) The sebum secretion inhibitory effect of the skin lotion (skin lotion) containing the oak leaf cedar balm extract was compared with the initial value, and the results are shown in Table 4 below.

Sebum secretion inhibitory evaluation Sebum amount (mg / cm ² ㆍ hr) forehead cheek Initial value 88.17 72.94 Week 1 79.49 53.75 2nd week 81.35 71.91 3rd week 78.14 63.98 4 weeks 67.55 61.81

From the above Table 4, it was confirmed that the skin lotion according to the present invention containing the eye side extract extract significantly inhibits sebum secretion at each facial region of those who suffer from sebum secretion.

Test Example 4: Efficacy of Acne Treatment of Skin Lotion Containing Natural Perfume Extract

Skin of Examples 1 to 3 and Comparative Examples 1 to 3 in order to measure the clinical effect on treatment or alleviation of acne of the plant extract-containing skin lotion cosmetic composition according to the present invention, which contains a natural anti-Euburn leaf extract Clinical evaluation of lotions was performed. Acne treatment status was visually and photographically evaluated for 30 women in their 20s with acne development using skin lotions having the compositions of Examples 1 to 3 and Comparative Examples 1 to 3 twice daily for 30 days. The effect was determined by the above, and the results are shown in Table 5 below.

Acne treatment effect Good effect Effect slightly no effect Worse system Example 1 6 (40%) 7 (47%) 2 (13%) - 15 people Example 2 8 (53%) 6 (40%) 1 (6.7%) - 15 people Example 3 8 (53%) 7 (47%) - - 15 people Comparative Example 1 - 6 (40%) 7 (47%) 2 (13.3%) 15 people Comparative Example 2 - 7 (47%) 8 (53%) - 15 people Comparative Example 3 - 7 (47%) 8 (53%) - 15 people

From Table 5 above, it was found that the skin lotion according to the present invention containing the white matter extract has an excellent acne treatment to alleviate the relief.

Test Example 5 Satisfaction Satisfaction

For 50 women aged 25 to 40 years, the skin lotions of Examples 1 to 3 and Comparative Examples 1 to 3 were used for 1 week, and then the odor satisfaction was scored according to the following criteria. Table 6 shows.

Example 1 Example 2 Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 satisfaction 3.9 3.5 3.0 2.6 2.4 2.3

* Based on scoring: 5- Very good 4- Good 3- Normal 2- Inferior 1- Very poor

As can be seen from Table 6 above, the skin lotions of Examples 1 to 3 containing ocular cedar leaf natural fragrance extract provide a satisfactory odor, whereas the comparative examples contain tea tree oil or clover leaf oil. Skin lotions of 1 to 3 did not appear to provide a satisfactory odor.

Hereinafter, the external preparations for the skin of various formulations containing the oak leaf of the natural cedar extract belonging to the scope of the present invention can be prepared by a conventional method, and the typical formulation examples will be described.

Formulation Example 1: Flexible Lotion

ingredient Content (% by weight) glycerin 5.0 1,3-butylene glycol 3.0 Oleyl alcohol 0.1 Monolauric acid polyoxyethylene sorbitan (20E.O) 1.5 Polyoxyethylene lauryl ether 0.5 ethanol 8.0 Spices 0.05 antiseptic Quantity Natural White Oil Extract 0.5 Purified water Remaining amount Sum 100

Formulation Example 2: Convergence Lotion

ingredient Content (% by weight) Citric acid 0.1 Sorbitol 2.0 glycerin 3.0 Polyoxyethylene oleyl ether 1.0 ethanol 12.0 Spices 0.1 antiseptic Quantity Natural White Oil Extract 0.01 Purified water Remaining amount Sum 100

Formulation Example 3: Cream

ingredient Content (% by weight) Squalane 5.0 Stearic acid 8.0 vaseline 2.0 Monostearic acid polyoxyethylene sorbitan (20E.O) 1.5 Self-emulsifying glycerin monostearate 2.5 Propylene glycol 4.0 glycerin 5.0 Natural White Oil Extract 0.025 antiseptic Quantity Antioxidant Quantity Purified water Remaining amount Sum 100

Formulation Example 4: Lotion

ingredient Content (% by weight) Stearic acid 1.0 ethanol 1.5 vaseline 2.0 Squalane 3.0 Liquid paraffin 5.0 Monooleic acid polyoxyethylene sorbitan (6E.O) 2.0 Propylene glycol 4.0 glycerin 3.0 Triethanol amine 1.0 Natural White Oil Extract 0.2 Spices 0.3 antiseptic Quantity Antioxidant Quantity Purified water Remaining amount Sum 100

Formulation Example 5 Latex Liquid Foundation

ingredient Content (% by weight) Stearic acid 2.0 Cetylstearyl ethanol 0.5 Liquid lanolin 2.0 Myristic acid isopropyl 5.0 Liquid paraffin 3.0 Carboxyethyl Cellulose Sodium 0.2 Bentonite 0.5 glycerin 3.0 Titanium dioxide 8.0 Talc 4.0 Coloring pigment Quantity Spices 0.2 antiseptic Quantity Antioxidant Quantity Natural White Oil Extract 0.2 Purified water Remaining amount Sum 100

Formulation Example 6: Soap

ingredient Content (% by weight) Palm oil fatty acid 1.0 Titanium dioxide 0.3 Ethylenediamine Tetraacetate Disodium 0.1 Spices 0.7 Pigment Quantity Natural White Oil Extract 0.1 Soap Chip Base Remaining amount Sum 100

As described above, the acne cosmetic composition according to the present invention containing the natural fragrance extract derived from the oak leaf as a main component exhibits an excellent proliferation inhibitory effect against propionibacterium acne, the acne causing bacterium, and is related to sebum oversecretion. It exhibits excellent inhibitory activity against 5α-reductase and is therefore very useful for the treatment, prevention and / or alleviation of acne.

Claims (3)

As an active ingredient for improving acne skin, it contains natural perfumed oil extracted from the leaves of Thuja koraiensis nakai in an amount of 0.0001 to 5% by weight based on the total weight of the skin external preparation. Acne cosmetic composition. (delete) The acne cosmetic composition according to claim 1, wherein the skin external preparation is any one selected from the group consisting of skin lotion, cream, lotion, foundation, pack, face wash foam and soap.