KR20040056079A - Cosmetic composition for preventing and therapeutic for a pimple - Google Patents

Abstract

PURPOSE: Provided is a therapeutic cosmetic composition containing Viola variegata Fisch extract for preventing and treating a pimple. The composition has excellent antibacterial effect on Propionibacterium acnes causing a pimple, and anti-inflammatory activity. CONSTITUTION: A cosmetic composition for the prevention and treatment of a pimple is characterized by containing 0.001-20.0 wt.% of Viola variegata Fisch extract, based on the dried weight of the composition. The extract of Viola variegata Fisch is obtained by the steps of: drying and pulverizing Viola variegata Fisch; extracting it with a solvent; filtering the extract; distilling the filtrate under reduced pressure and concentrating it; and freeze-drying the concentrate and pulverizing it.

Description

Cosmetic composition for preventing and treating acne {Cosmetic composition for preventing and therapeutic for a pimple}

The present invention relates to a cosmetic composition for preventing and treating acne, and more particularly, has excellent antibacterial activity against propionibacterium acnes known as a causative agent for worsening acne, and various skin problems such as acne. The present invention relates to a cosmetic composition containing a substance having an excellent anti-inflammatory effect such as suppressing or alleviating inflammation caused when it occurs.

Acne is a skin disease caused by sebaceous gland abnormalities around the hair follicles. Under normal conditions, sebum rises up along the hair follicle wall and is discharged out through the skin. Therefore, acne occurs mainly on the face, scalp, neck, chest, back, upper limbs and shoulders where sebaceous glands are concentrated. Statistically, Koreans are known to have more than one acne lesion on their face in almost everyone between the ages of 15 and 20. Westerners begin to appear at ages 10 to 13 and Koreans at ages 15 to 17 years of age, lasting 5 to 10 years, and slowly disappearing in their early 20s. In some cases, however, acne persists until late 20's or 30's, and sometimes afterwards. Regardless of gender, it occurs at about the same rate, but males have more severe and longer lasting acne than women. In the case of female acne, acne has worsened due to hormonal changes or incorrect cosmetic use. In particular, acne caused by the use of cosmetics often lasts until adulthood.

Acne is caused by a combination of basic causes, including the release of androgen hormones, increased sebum secretion, changes in hair follicles, and bacterial activity. Androgen hormones normally act to enlarge the sebaceous glands. In acne patients, the sebaceous glands are excessively stimulated by the androgen hormones, causing the sebaceous glands to enlarge and eventually increase sebum secretion. In women, symptoms may worsen just before menstruation, which is also associated with the action of androgen hormones. Sebum produced excessively by hormonal stimulation not only rises to the surface of the skin along the hair follicle wall and out of the skin, but also accumulates in the hair follicle, leading to acne. Normally, the dead cells in the hair follicles are taken out of the skin one after another, but in puberty acne patients, these cells drop out so quickly that they cannot all escape, become entangled with each other and clump together with sebum in the hair follicles to block the hair follicles. A clogged hair follicle is a good environment for germs to grow. In particular, a bacterium called propionibacterium acnes is the main cause of acne when it grows on sebum. In normal people without acne, the bacteria are present on the skin and do not cause any problems. When they accumulate, sebum eats and grows quickly, and the various chemicals produced and released during metabolism cause inflammation of the hair follicles and surrounding skin. There is also no clear scientific evidence, but it is almost accepted that acne and genetic factors are involved. Acne patterns, duration, and severity among the same family members are often similar to each other, and the incidence of acne in Caucasians is relatively higher than that of blacks and Asians. It suggests that you are involved. Recently, the occurrence and deterioration of acne caused by cosmetics are frequent, but so-called cotton-generating cosmetics such as makeup cosmetics, night creams, moisturizers, and perfumes are not good for acne patients.

Acne can be divided into microvesicles, non-inflammatory (inflammatory), and inflammatory depending on symptoms. Small vesicles appear early in the acne and are produced as the skin is stretched by clogged sebum masses that are blocked by small follicles, and in some cases are small and invisible to the naked eye. Non-inflammatory (sclerosic) occurs as the vesicles continue to develop into open and closed capillaries (baekdu). The open type means that the accumulation in hair follicles is exposed to the surface of the skin and is black. The closed type refers to the appearance of skin color or white bumps because the sebum mass is not exposed to the skin surface. Inflammatory acne is an inflammatory response to the surrounding skin tissue as the Phoebe substance escapes from the scalp and becomes red due to inflammation. There are three basic types of inflammatory acne: papules, pustules, and nodules. Papules are small, hard, reddish lesions that can appear as non-inflammatory and inflammatory intermediate forms, and are accompanied by mild inflammation. Pustules are similar in size to papules and are small and round in shape, but differ from papules in their inclusions. The bacteria are not in the farm, and the inflammatory reaction is caused by chemical stimulation by components such as free fatty acids in sebum. Nodules are the most severe form of acne, which are large, inflamed and contain large amounts of pus. The nodule forms the pus by the contents of the scrim come out and induce an immune response to the surrounding skin tissue. It is relatively deep in the skin and can be painful and has a high chance of leaving acne scars during the healing process compared to other lesions. As such, acne can be divided into papular acne, nodular acne, and coagulated acne. Papules Pulmonary acne is the most common form of acne, the appearance of a mix of cotton, papules, pustules. Therefore, papules of acne are acne, which is a mixture of non-inflammatory and inflammatory acne, and is called general acne. The severity of papule acne is known as the ratio of papules to pustules, which are more severe than papules or pustules. Nodular acne is a more severe form than papule-acne acne, characterized by a nodule that is larger in size, ugly, and accompanied by pain. Nodular cystic acne is a more severe form of nodular acne, which is a form of acne with nodules and cysts containing a large amount of pus. Finally, coagulated acne, the most severe form of acne, is a form of severe bacterial skin infections with inflammatory acne lesions. This occurs mainly on the back, chest, buttocks, etc. And often require surgical surgery.

Acne treatment may be effective by inhibiting one or more causes of acne, such as excessive sebum secretion, abnormal dropout of skin cells in hair follicles, and propagation of propionibacterium acnes. Benzyl Peroxide has the effect of removing propionibacterium acnes. Because it takes at least two weeks to have some effect, it must be treated continuously, and since it does not affect sebum production or skin cell detachment of the hair follicle wall, it is more effective to combine with other drugs. Salicylic acid helps to maintain a normal skin cell detachment cycle. Especially for mild acne, salicylic acid opens clogged hair follicles to help treat and prevent acne. However, these two ingredients have the drawback that acne may return if you stop using it. Antibiotics act to remove propionibacterium acnes and come in both external and oral dosage forms. In the case of external preparations, there are few side effects, but there is a limit to skin absorption, which makes it difficult to remove bacteria deep in the skin. Oral doses are more effective in severe acne. Retinoids are derived from vitamin A and have a variety of effects on the skin. External retinoids treat acne by reducing sebum production and normalizing the loss and growth of skin cells in hair follicles. Oral retinoids can improve all the causes of acne by reducing sebum production, improving skin cell detachment in hair follicles, and reducing propionibacterium acnes. However, pay close attention to side effects and should not be used during pregnancy.

Inflammation refers to a series of defenses aimed at minimizing the response and restoring the damaged area when a cell or tissue is damaged by any cause.Trains, burns, frostbite, and radiation are the causes of inflammation. Physical causes, etc., chemical factors such as acids, and immunological factors due to antibody reactions are also caused by vascular and hormonal imbalances. The anti-inflammatory effect is called to eliminate the cause of inflammation, to reduce the biological response and symptoms to eliminate inflammation.

The cause of skin side effects is always latent in cosmetics and various studies have been conducted to solve this problem. Materials used in various fields for anti-inflammatory purposes until now are nonsteroidal flufenamic acid, ibuprofen, benzidamine, indomethacin, etc. Prednisolone, dexamethasone, and the like, and allantoin, azulene, aminokiproic acid, hydrocortisone, licorice acid and derivatives thereof are known to be effective in anti-inflammatory. However, indomethacin is a substance that can not be used in cosmetics, hydrocortisone is limited in use, and licorice and its derivatives are difficult to stabilize or have poor solubility, which makes it difficult to achieve practical effects due to the concentration limitation. The anti-inflammatory agents known to date have most problems in terms of skin safety and stability in the formulation of cosmetics, and their use is limited.

Accordingly, an object of the present invention is to provide a composition that can easily prevent and treat acne by solving the above-described disadvantages of conventional acne treatments.

Another object of the present invention is to provide a cosmetic composition that can be used in the form of a cosmetic composition for preventing and treating acne.

Furthermore, another object of the present invention is to provide a manufacturing method that can easily prepare the main components of the composition for preventing and treating acne.

The present inventors have performed a number of studies to achieve the object of the present invention as a result, there is an excellent antimicrobial action against propionibacterium acnes acting as the main causative agent of acne exacerbation, The present invention has been completed by providing a method for finding a natural product containing a highly safe substance and effectively extracting the active ingredient therefrom and a composition containing the same.

One composition for achieving the above object of the present invention has excellent antibacterial action against propionibacterium acnes acting as the main causative agent of acne exacerbation, violet violet material having high safety even for long-term use Extracted from Viola mandshurica W. BECKER, fruit extracts are contained in an amount of 0.001% to 20.0% by weight on a dry weight basis, and prevention of acne to which a cosmetic composition may be added. It is characterized by providing a cosmetic composition having an effective effect on treatment.

Violet, which is a major component of the present invention, is also called a purple flower, oranca, ring flower, and is a perennial plant of violets commonly native to sunny places of roadsides or grasslands all over the country. There are no stalks and leaves and flower beds with long petioles grow from the roots. A peduncle of 5-20cm in height comes out in April-May, and a dark purple flower grows, and the fruit ripens in July-August. There are as many as 64 species of violets of the same genus all over Korea. The flowers come in a variety of colors, including purple, purple, reddish purple, white, and yellow. In Dong-gam Bogam, violets are called self-designated, and they are indigenous to Joseon and Manju mountains and have at least 20 species of the same genus. The flower stalk is the same and has the petals and the honey bag and droops down. Young leaves are blanched and eaten as herbs. For medicinal use, the root part is mainly used for hemostasis, typhoon, and fluency. In general, violet is known to have a strong fever, anti-inflammatory, antibacterial action, promote the circulation of lymph, cleanse blood, heal inflammatory diseases. Its main ingredients include cherotic acid and flavones, and especially the leaves contain vitamin C, which is four times the amount of oranges (Korean herb-protected herbal medicine, book publishing reason, 2001; Korea's resource plant, Seoul National University Press, 1996).

In addition, the violet as described above is a plant that has been used in herbal medicine for a long time, even in the intake of the body has been confirmed that the stability to the human body and is almost excellent in safety with little irritation to the skin and mucous membranes.

Korean Patent Application No. 97-127343 (Pacific, Oct. 21, 1997) contains skin subtractive effect of tannin component and tannin component by containing phalaenopsis, a subspecies of violet, and other tannin-based plant extracts and metal ion sequestrants. A human cleanser composition is disclosed which is characterized by prevention of discoloration.

Japanese Patent Application Publication No. 5-255060 (Younggwangdang, 1993.10.5) discloses a cosmetic composition containing Viola tricolor extract that promotes skin metabolism and has anti-inflammatory and wound healing effects. Japanese Patent Application Laid-Open No. 2001-48773 (Naris Cosmetics, 2001.2.20) discloses a whitening cosmetic composition containing violet, tricolor violet extract and the like to suppress skin damage by ultraviolet rays.

By the way, the patent application No. 97-127343 seeks skin beauty effects and feelings using tannin components obtained from several plant extracts, including phalaenopsis flowers, etc., but specifically mentions the effects of acne relief or effects on acne skin. There is no description of antibacterial effects such as inhibition of propionibacterium acnes, which is a cause of acne, and Japanese Patent Application Laid-Open Nos. 5-255060 and 2001-48773 also have a variety of violet and tricolor violets. The effect of extract on skin beauty or whitening is proposed, but there is no consideration of acne and the antimicrobial effect is not mentioned as the purpose and technical content of the present invention. little.

The content of violet extract in the acne prevention and treatment composition according to the present invention contains 0.001% to 20.0% by weight, preferably 0.005% to 10.0% by weight. If the amount is less than 0.005% by weight, the antimicrobial activity against propionibacterium acnes may be somewhat insufficient, and when more than 10.0% by weight is used, it is difficult to prepare the composition and ensure long-term stability. In addition, the concentration of less than 0.005% by weight of the anti-inflammatory effect is low, so it is difficult to expect the inhibitory effect of the inflammatory phenomenon caused by skin troubles, the efficacy of the present skin composition is halved.

Meanwhile, the violet extract-containing skin composition of the present invention can be applied to any shape, such as liquid, cream, paste or solid, so that creams, lotions, skin lotions, packs, foundations, makeup bases, soaps, facial foams, body cleansers, etc. It is also applicable to facial products such as face washes, cleaning agents and shampoos and rinses, and, if necessary, components commonly used in external preparations for skin within the scope of not impairing the effects of the present invention, for example, aqueous components, oily components. It can be mix | blended with surfactant, a moisturizer, a thickener, a preservative, a fragrance | flavor, a pigment | dye, etc.

It is also possible to use in combination with antibacterial agents such as benzoyl peroxide, tetracycline, erythromycin, triclosan, hormones such as androgen and steroids, and vitamin A derivatives such as retinoids, which have been used in conventional acne treatments.

According to another configuration of the present invention, the active ingredient of the violet according to the present invention can be extracted by the extraction method of the plant extract using a conventional solvent, more specifically can be extracted by the following method. In other words,

a) powdering dried violet ( Viola mandshurica W. BECKER);

b) extracting the powdered violets with a solvent;

c) filtering the extract filtrate;

d) concentration of the filtrate by distillation under reduced pressure;

e) freezing and drying the concentrated filtrate to powder again.

In addition, the solvent used in the extraction is purified water, methanol, ethanol, propanol, isopropanol, butanol, acetone, glycerol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, hexane, diethyl ether, dichloromethane It may be selected from the group consisting of one or more.

Violet extract of the present invention is configured as described above has a very excellent antibacterial effect against propionibacterium acnes (propionibacterium acnes) has an excellent effect when applied to acne skin, especially papules, pustules appearing at each acne stage It can have a quick calming effect on inflammation such as nodules.

Hereinafter, the present invention will be described in detail through Examples and Comparative Examples. However, these examples are only for illustrating the present invention and the present invention is not limited thereto.

Example 1

Extraction of Active Components from Violets

Crushed violet (dried herb) powder to 100 g of powdered and 1000 ml of methanol (80%) was added, immersed at room temperature for 4-5 days, extracted and filtered twice. The filtrate was concentrated by distillation under reduced pressure and frozen again. Drying gave 21.4 g of powder.

Test Example 1

Determination of Antimicrobial Activity

Antibacterial activity is measured by a broth dilution method using a 96-well microplate. Heat-sterilized Brainheart Infusion Broth medium (BHI broth) is injected into each well of a 96-well microplate, followed by two-fold dilution of the extract sample. After injecting the test cell suspension into all wells, the cells are incubated anaerobicly at 37 ° C. After incubation for 3 to 4 days, the alamar blue solution is dispensed into each well and incubated for about 1 hour, and then the color development is observed to determine the concentration of the color change from blue to pink as the MIC value. .

Antimicrobial Activity against Propionibacterium acnes sample MIC (ppm) ATCC 6919 Violet extract 10 to 30 Triclosan 20 to 40 Salicylic acid 2000 Purified water -

As shown in the table, violet extract showed a very good antibacterial activity compared to salicylic acid, which has been used as an active ingredient in conventional acne treatments, and showed a comparable effect even when compared to triclosan, which is known to have a broad antibacterial range. Therefore, when the violet extract of the present invention is formulated into the skin composition for the purpose of preventing and treating acne is expected to exhibit a very good antibacterial effect.

Test Example 2

Measurement of anti-inflammatory effect

The extract sample and the control sample are dissolved in a suitable concentration in purified water or propylene glycol. Each sample solution is reacted with a hyaluronidase enzyme solution, calcium chloride (CaCl2), hyaluronic acid, and the like for a predetermined time, and then the change in absorbance is measured at 585 nm. At this time, the degree of inhibition of enzymatic activity of pure methanol was measured together, and the degree of inhibition of the extract sample was measured by the relative inhibition rate, and the anti-inflammatory effect was evaluated by this relative inhibition rate value (Anonymous, Methods of Enzymatic Analysis, Bergmeyer, HU 1974, P). 944)

Anti-inflammatory effect through hyaluronidase inhibitory activity sample % Activity inhibition Violet extract 132.7 Allantoin 71.3

As shown in the table above, violet extract was found to have a relatively higher anti-inflammatory effect compared to allantoin (Allantoin), which is known to have an excellent soothing effect upon inflammation. From the above results, when the violet extract is used in combination with the skin composition, it is expected that the inhibition and alleviation of the inflammatory phenomenon accompanying the occurrence of various skin problems such as acne can be expected.

Test Example 3

Evaluation of Antimicrobial Activity of Violet Extract Containing Composition

In order to evaluate the antimicrobial effect of the violet extract-containing composition according to the present invention, the facial soaps of Example 1 and Comparative Examples 1-2 were prepared using the composition shown in Table 3, and the paper-disk method was used. Each composition was dissolved in propylene glycol, diluted with a 10% solution, and the paper-disk (diameter 8 mm) was immersed in the solution for the same time, taken out, and placed on agar plate inoculated with the test bacteria and incubated. After incubating for 1 to 2 days, the antimicrobial effect of the composition was qualitatively determined by measuring the presence or absence of the development low zone around the paper-disk and the results are shown in Table 4.

Composition of face wash soap for measuring the effect (% by weight) Name Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Palm oil fatty acid 2.0 2.0 2.0 2.0 glycerin 0.5 0.5 0.5 0.5 Titanium dioxide 0.3 0.3 0.3 0.3 EDTA-2Na 0.1 0.1 0.1 0.1 Spices Quantity Quantity Quantity Quantity Color Quantity Quantity Quantity Quantity Violet extract 1.0 0.1 0.001 - Soap Chips (Base) Remaining amount Remaining amount Remaining amount Remaining amount Sum 100 100 100 100

Antimicrobial Activity against Propionibacterium acnes sample Diameter of growth inhibition zone (mm) ATCC 6919 Example 2 (10% solution) 17 Comparative Example 1 (10% solution) 11 Comparative Example 2 (10% solution) Lowland not formed Comparative Example 3 (10% solution) Lowland not formed

As a result of evaluating the antimicrobial effect on the dilute solution of the face-wash soap containing the extract of the present invention, it showed a very excellent effect as shown in Table 4. According to these results, the skin composition containing the violet extract of the present invention, that is, it can be expected to prevent and treat the acne of the facial soap.

Test Example 4

Evaluation of Acne Treatment Effects

In order to evaluate the clinical effect on the prevention and treatment of acne of the violet extract-containing composition according to the present invention, the skin lotion of Example 3 and Comparative Example 3-4 was prepared with the composition as shown in Table 6, and experimented with acne holders. In other words, for 30 women in their 20s with acne symptoms, the skin lotion having the composition shown in Table 6 was used twice a day for 3 weeks, and the condition of acne was classified according to the criteria of Table 5, and compared by visual and photographing. It judged through and the result is shown in Table 7.

Rating Skin condition 0 normal One Small number of cotton veins 2 Medium cotton patch without inflammation 3 Scalp of inflammatory papules 4 Small nodules and inflammatory scalp 5 Papular nodules 6 Moderate inflammatory nodules 7 Large number of inflammatory nodules 8 Severe nodular scalp 9 Severe cystic and inflammatory nodules

※ If the skin condition of acne in clinical trial subjects showed more than 3 grade reduction compared with the beginning of the experiment, it is excellent. If 1 ~ 2 grade is decreased, it is good. If there is no difference between grades, it is judged as worse. It was.

Composition of skin lotion for measuring effect (% by weight) Name Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 ethanol 5.0 5.0 5.0 5.0 glycerin 3.0 3.0 3.0 3.0 1,3-butylene glycol 3.0 3.0 3.0 3.0 Polyoxyethylene alkyl ether 0.5 0.5 0.5 0.5 Spices Quantity Quantity Quantity Quantity Violet extract 1.0 0.1 0.001 - Purified water Remaining amount Remaining amount Remaining amount Remaining amount Sum 100 100 100 100

Evaluation of Acne Treatment Effects division Grading system Great Good no effect worse Example 3 13 (65) 5 (25%) 2 (10%) - 20 (100%) Comparative Example 4 1 (10%) 9 (45%) 10 (50%) - 20 (100%) Comparative Example 5 - 2 (10%) 16 (80%) 2 (10%) Comparative Example 6 - 2 (10%) 15 (75%) 3 (15%) 20 (100%)

Acne prevention and treatment of the dermatological composition according to the present invention was found to have a very good effect as shown in Table 7.

Example 4-8

Hereinafter, the composition of the various types of cosmetic composition containing a Namsil extract obtained according to Example 1 of the present invention is shown in Table 8-12.

Cream (% by weight) Stearic acid 8.0 glycerin 5.0 Squalene 5.0 Propylene glycol 3.0 Self-emulsifying glycerin monostearate 2.5 Monostearic acid polyoxyethylene sorbitan (20 E.O.) 1.5 Vaseline 1.5 Spices Quantity antiseptic Quantity Violet extract 1.0 Purified water Remaining amount Sum 100

Lotion (% by weight) Liquid paraffin 5.0 glycerin 3.0 Squalene 3.0 Propylene glycol 3.0 Vaseline 2.0 ethanol 2.0 Monostearic acid polyoxyethylene sorbitan (20 E.O.) 1.5 Triethanolamine 0.5 Spices Quantity antiseptic Quantity Violet extract 1.0 Purified water Remaining amount Sum 100

Liquid foundation (% by weight) Titanium dioxide 8.0 Myristic acid isopropyl 5.0 Talc powder 5.0 Liquid lanolin 3.0 Liquid paraffin 3.0 glycerin 3.0 Bentonite 0.5 Cetostearyl alcohol 0.5 Carboxymethyl Cellulose Sodium 0.3 Coloring pigment Quantity Spices Quantity antiseptic Quantity Violet extract 1.0 Purified water Remaining amount Sum 100

Essence (% by weight) Sorbitol 8.0 Polyoxyethylene glycol 1500 6.0 ethanol 5.0 glycerin 3.0 1,3-butylene glycol 3.0 Polyoxyethylene oleyl alcohol ether 1.0 olive oil 0.3 Hyaluronic acid 0.2 Spices Quantity antiseptic Quantity Violet extract 1.0 Purified water Remaining amount Sum 100

Cleansing Foam (% by weight) Myristic acid 15.0 Stearic acid 12.0 Potassium hydroxide 5.0 glycerin 3.0 1,3-butylene glycol 3.0 Monostearic acid polyoxyethylene sorbitan (20 E.O.) 2.0 EDTA-2Na 0.1 Pigment Quantity Spices Quantity antiseptic Quantity Violet extract 1.0 Purified water Remaining amount Sum 100

As described above, the method of extracting the active ingredient of violet according to the composition of the present invention and the cosmetic composition containing the same provide a method of easily obtaining a violet active ingredient exhibiting an excellent effect on the prevention and treatment of acne, It is a useful invention to provide a cosmetic composition which can be contained in a ratio to prevent acne or to treat with an excellent effect.

Claims (5)

Viola mandshurica W. BECKER extract, characterized in that it contains a conventional cosmetic composition is a cosmetic composition for preventing and treating acne. The cosmetic composition for preventing and treating acne of claim 1, wherein the violet extract contains 0.001% to 20.0% by weight on a dry weight basis. The cosmetic composition according to claim 1 or 2, wherein the cosmetic material is a cream, lotion, skin lotion, pack, foundation, essence, cleansing foam, soap, facial foam, or body cleanser. a) powdering dried violet ( Viola mandshurica W. BECKER); b) extracting the powdered violets with a solvent; c) filtering the extract filtrate; d) concentration of the filtrate by distillation under reduced pressure; e) extracting the violet active ingredient, characterized in that it comprises the step of lyophilizing the concentrated filtrate to powder again. The method of claim 4, wherein the extraction solvent is purified water, methanol, ethanol, propanol, isopropanol, butanol, acetone, glycerol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, hexane, diethyl ether, dichloro Extracting a violet active ingredient, characterized in that at least one solvent selected from the group consisting of methane.