KR20160079473A - Preparation method of cleaning or disinfecting composition having antiviral activity by containing green tea extract as active component - Google Patents
Preparation method of cleaning or disinfecting composition having antiviral activity by containing green tea extract as active component Download PDFInfo
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- KR20160079473A KR20160079473A KR1020140190860A KR20140190860A KR20160079473A KR 20160079473 A KR20160079473 A KR 20160079473A KR 1020140190860 A KR1020140190860 A KR 1020140190860A KR 20140190860 A KR20140190860 A KR 20140190860A KR 20160079473 A KR20160079473 A KR 20160079473A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
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- C11D3/382—Vegetable products, e.g. soya meal, wood flour, sawdust
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
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Abstract
The method for preparing a composition for cleaning or disinfecting according to the present invention comprises the steps of (a) preparing a green tea extract using green tea leaves, green tea stalks and green tea seeds, (b) adding an antioxidant and a stabilizer to the green tea extract, And (c) preparing a composition for cleaning or disinfection by adjusting the pH of the green tea mixture. The composition for cleaning or disinfection is prepared using a natural green tea extract to exhibit high antiviral activity, Or the use of the chemical added to the composition for disinfection is reduced, so that the composition for cleaning or disinfecting can easily be used for people with skin diseases because it is hypoallergenic to the human body.
In addition, the composition for cleaning or disinfection produced the antioxidant and antiseptic activity including various natural additives and porous beads, because the antioxidant and the stabilizer were treated with the green tea component to increase the shelf life, Which can be used as a hand sanitizer, a human body cleanser, a bottle disinfectant, or an animal disinfectant, which is highly dependent on a vaccine and can be used easily and quickly for various respiratory diseases with limited effect of existing therapeutic agents A composition can be prepared.
Description
The present invention relates to a method for preparing a composition for cleaning or disinfection having antiviral activity and long-term storage stability.
Influenza is a type of respiratory disease virus that is spread not only to humans but also to various kinds of animals as well as livestock such as pigs, chickens, ducks or horses.
In particular, in 2009, pandemic highly pathogenic viruses such as novel swine-origin influenza A (H1N1) appeared, resulting in massive casualties and massive economic losses.
The above-mentioned influenza virus belongs to Orthomyxoviridae and has eight negative strand RNA fragments of PB2, PB1, PA, HA, NP, NA, M and NS.
Of the above negative strand RNA fragments, HA is a gene encoding hemagglutinin (hereinafter referred to as "HA") which is a transcriptional envelope protein, and is an antigen of virus surface which is involved in adhesion to influenza virus mammalian cells NA is a gene encoding neuraminidase (hereinafter referred to as "NA "), plays an important role when a virus enters or leaves a host cell, And the virus is transformed through the process of antigenic feces or antigen urine. Therefore, the treatment is not easy.
The above-described influenza viruses generally exhibit various symptoms and severity according to the subtypes prevalent in winter, and there are various viruses ranging from those having no pathogenicity to those having a high mortality rate of 60%. Generally, Pneumonia, and cardiopulmonary complications, resulting in a high mortality rate. The highly pathogenic avian influenza virus is classified as a Type 1 infectious disease in Korea and is classified as List A in the International Bureau of OIE (OIE).
In 2009, the case of H1N1 influenza was lower than that of avian influenza, but it was highly contagious, causing economic and social problems.
The above-mentioned avian influenza virus is a common infectious virus, and the main propagation factor is contaminated feces, and it can be spread by droplet, air, water and the like. In addition, the avian influenza virus may infect humans through intermediate hosts such as pigs and chickens. In the middle host, the avian influenza virus may cause gene mutation or recombination with other viruses, It spreads to people through respiratory infections and causes flu.
However, the development of effective preventive vaccines against newly emerging influenza viruses is insufficient and the limitation of current therapeutic agents remains a problem.
Examples include ion channel inhibitors such as Amantadine and Rimantadine, Oseltamivir, a neuraminidase (NA) inhibitor, which have been developed in the past, Zanamivir et al. Have already reported mutagenic strains that are resistant to the development of new antiviral interventions.
Recently, it has been reported that personal hygiene habits such as wearing masks and hand hygiene can significantly suppress the spread of viruses, and it is known to be an effective method for pandemic influenza, such as pandemic influenza, because it quickly and easily blocks virus transmission .
Accordingly, after the H1N1 influenza became popular, various hand disinfectants and hand cleansers appeared on the market like pussy. Currently, the KFDA classifies hand sanitizer as quasi-drug and hand cleanser as cosmetics.
A common hand sanitizer currently marketed is one that uses one of ethanol, isopropanol, benzalkonium chloride, cresol, hydrogen peroxide as a main component and protects skin damage caused by alcohol. It is manufactured using propylene glycol, glycerin, tocopherol and the like , And the gel type containing alcohol in a ratio of 60 to 70% is most common as a hand disinfectant that does not use water. The hand-cleansing agent is used as an auxiliary to hand-cleansing as a cosmetic product, and is generally hand-made water-based hand-washing, hand wash, and hand washing soap.
The above-mentioned hand cleaner and hand sanitizer usually have a shelf life of 2 to 3 years, but it is recommended to use it within 6 months after opening. It is not easy to preserve and ethanol, isopropanol, benzalkonium chloride, cresol The use of synthetic chemicals such as water, hydrogen peroxide, and the like has caused skin problems such as allergies and skin troubles.
Accordingly, studies on natural detergents or disinfectants using natural substances have been actively conducted, and in particular, the number of research papers on various physiological activities of green tea having antiviral activity has been rapidly increasing, and in particular, influenza viruses, Various studies have been conducted on a method for producing a detergent and a disinfectant by using an extract of green tea having an antiviral activity against retroviruses such as retroviruses, fstivinaviruses and herpes viruses.
However, the detergents and disinfectants using the green tea extracts described above are made of natural materials and have difficulty in storage due to short shelf life due to difficulty in storage and distribution due to difficulty in mass production. In order to increase shelf life, There is a problem in that it is limited in use to a person having skin allergy due to a synthetic chemical ingredient contained in the manufactured detergent and disinfectant.
DISCLOSURE Technical Problem The present invention has been conceived to solve the problems of the prior art as described above, and it is an object of the present invention to provide an antioxidant and a stabilizer to the green tea extract to improve the stability of the green tea extract and the shelf life of the green tea extract, And to provide a method for manufacturing a composition for cleaning or disinfecting which is improved in durability.
Another object of the present invention is to provide a technique for preventing spread of viruses and germs by using the composition for cleaning or disinfecting, which is manufactured by the method for manufacturing a cleaning or disinfecting composition and has improved quality life, for use in disinfection and multi- will be.
It is another object of the present invention to provide an antimicrobial and antimicrobial activity equivalent to or better than conventional hand and multi-use detergents by using natural ingredients such as green tea extracts for the production of compositions for cleaning or disinfecting, And to provide a method for manufacturing a cleaning or disinfecting composition that is environmentally friendly and less harmful to human health.
In order to accomplish the above object, the present invention provides a method for preparing a green tea extract, comprising: (a) preparing a green tea extract using green tea leaves, green tea stalks and green tea seeds, (b) adding an antioxidant and a stabilizer to the green tea extract, And (c) adjusting the pH of the green tea mixture to prepare a composition for cleaning or disinfecting.
The method for manufacturing a cleaning or disinfecting composition according to claim 1, wherein the step (a) comprises the steps of:
1) extracting green tea leaves, green tea stalks and green tea seeds and boiling them with steam; 2) drying the green tea leaves, green tea stalks and green tea seeds, which have been matured in step 1) Preparing green tea powder by pulverizing the dried green tea leaves, green tea stalks and green tea seeds, 4) preparing a hot water extract by hot water extraction of the green tea powder, and 5) filtering the filtered hot water extract, Lt; / RTI >
In addition, the green tea extract may contain at least one selected from the group consisting of catechin, epicatechin, epigallocatechin (EGC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG) a flavonoid derivative, and is contained in an amount of 20% or less based on the total weight of the composition.
The antioxidant is at least one compound selected from the group consisting of tocopherol, ascorbic acid, ascorbic acid derivatives, gallic acid, quercetin, flavonol, flavonoid, catechol, ethoxyquin and lignan glycosides, And 1.5% or less.
The stabilizer may be selected from the group consisting of β-cyclodextrin, citric acid, magnesium chloride, MgCl 2, ethylenediaminetetraacetic acid (EDTA salt), and dibutyl hydroxy toluene (BHT) And is contained in an amount of 1% or less based on the total weight of the composition.
In the step (b), at least one selected from the group consisting of isopropyl alcohol, ethanol, n-propanol, t-butanol, 2-butanol, pentanol, hexanol and povidone- .
Further, in the step (b), the porous beads, the natural fragrance or the natural additives are further mixed.
In addition, the composition for cleaning or disinfecting is characterized by having antiviral activity against enveloped virus.
In addition, the coat virus is characterized by being an influenza virus, a retrovirus, an epstein-barr virus or a herpes simplex virus.
The method for preparing a composition for cleaning or disinfecting according to the present invention can prepare a composition for cleaning or disinfection with an increased shelf life by mixing an antioxidant and a stabilizer in a green tea extract.
In addition, the composition prepared by the method for preparing a composition for cleaning or disinfecting according to the present invention not only increases the personal hygiene of various respiratory diseases with high vaccine dependence and limited therapeutic effect, It can be widely used for disinfection and cleaning purposes for personal use, business use, hospital use or shaft use, and especially for direct disinfection of animals, farmers, farm animals and farmers.
In addition, by using green tea, which is an eco-friendly material, it is possible to use people who have skin diseases easily for manufacturing a composition for cleaning or disinfection, and it is possible to promote the domestic green tea industry which has already reached internationalization level.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a process drawing showing a method for producing a composition for cleaning or disinfecting according to the present invention. FIG.
FIG. 2 shows the antiviral activity of EGCG, which is a major component of green tea extract, as a result of plaque inhibition experiment.
3 is a graph showing the results of the plaque formation experiment of Experimental Example 2. Fig.
Hereinafter, the present invention will be described in detail.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a process drawing showing a method for producing a composition for cleaning or disinfecting according to the present invention. FIG.
As shown in FIG. 1, the method for manufacturing a cleaning or disinfecting composition according to the present invention comprises the steps of (a) preparing a green tea extract using green tea leaves, green tea stalks and green tea seeds, (b) To prepare a green tea mixture, and (c) adjusting the pH of the green tea mixture to prepare a composition for cleaning or disinfecting.
In step (a), the green tea extract is prepared using green tea leaves, green tea stems, and green tea seeds. In order to prepare green tea extracts, green tea is prepared by fermenting green tea leaves, green tea stems, It is preferable that the active ingredient of the green tea is maintained as it is by preparing the green tea extract by heating to destroy the oxidizing enzyme and extracting it.
The step (a) comprises the steps of (1) collecting green tea leaves, green tea stalks and green tea seeds and boiling them with steam, (2) drying the green tea leaves, green tea stalks and green tea seeds 3) preparing green tea powder by pulverizing the green tea leaves, green tea stalks and green tea seeds dried in step 2), 4) preparing a hot water extract by hot water extraction of the green tea powder, and 5) Extracting the hot-water extract, and concentrating the extract to obtain a green tea extract. The green tea extract may be prepared by maintaining the active ingredient contained in green tea leaves, green tea stalks, and green tea seeds.
The step 1) is preferably performed by heating to 100 to 300 ° C so as to increase the content of the green tea active ingredient, followed by drying.
In addition, it is preferable to heat the green tea powder by heating the natural water to extract the hot water in the step 4). The natural water may be selected from the group consisting of sodium (Na), calcium (Ca) , Minerals such as magnesium (Mg), potassium (K), strontium (Sr), lithium (Li), chloride ion (C1), zinc (Zn) and manganese (Mn) So that the physical properties of the composition can be further improved.
Further, the present invention is not limited to the above-described method. For example, a green tea extract may be prepared by extracting a green tea powder by fermenting a fermented green tea powder obtained by fermenting raw materials such as molasses or sweet potato, Alternatively, the green tea extract may be prepared by various known methods such as a distillation extraction method and the like to prepare a composition.
The green tea extract prepared as described above contains catechin, epicatechin, epigallocatechin (EGC), epicatechin gallate (ECG), epigallocatechin gallate, EGCG), flavonoid derivatives, and the like, exhibit an antiviral activity of removing at least 90% of the virus so as to maintain a clean state.
Particularly, the antiviral active ingredients contained in the above-mentioned green tea extract can be used as an antiviral agent in the form of an antiviral agent such as influenza virus, retrovirus, epstein-barr virus or herpes simplex virus enveloped virus, and thus can effectively prevent disease caused by the virus by blocking the infection pathway of the virus. For this purpose, it is desirable to use a washing or washing machine having a most preferable physical property when the green tea extract is contained at 20% A disinfecting composition can be prepared.
The step (b) is a step of preparing a green tea mixture by adding an antioxidant and a stabilizer to the green tea extract. The antioxidant may be mixed with the green tea extract for the purpose of enhancing the safety of long-term storage of the composition for cleaning or disinfection have.
The antioxidant is a natural antiseptic agent. As the active ingredient contained in the cleaning or disinfecting composition described below is brought into contact with oxygen in the air to naturally absorb oxygen in the air, physical and chemical changes are caused by a chain reaction of radicals Oxidation occurs, oxidation occurs by complex reaction due to temperature change, homolytic cleavage of water molecules occurs by irradiation of light, and oxidation is caused by a very strong active radical complex reaction formed thereby The antioxidant is expected to be oxidized very rapidly due to its chemical structure. When several oxidizable substances are present together, the antioxidant is oxidized prior to other substances, thereby preventing the oxidation of other substances. To prevent oxidation products such as alcohols, aldehydes, and ketones from being generated W maintain the quality of the cleaning or disinfecting composition is prepared by increasing the shelf life and shows an effect of AS to maintain the antiviral activity of green tea extract.
The antioxidant showing such an effect is preferably mixed with an antioxidant including tocopherol, ascorbic acid, ascorbyl palmitate, gallic acid, quercetin, flavonol, flavonoid, ethoxy queen, lignan glycoside and mixtures thereof .
The above-mentioned antioxidants can prevent the oxidation of components contained in the composition for cleaning or disinfection, thereby improving shelf life and providing an effect of increasing the circulation period. It is also possible to provide antioxidants of natural antioxidants Is preferably used.
In addition, in this step, a stabilizer other than the antioxidant may be further added to increase the antiviral activity and persistence of the green tea extract and to further improve the stability of the composition to be stored for a long period of time. In particular, Thereby preventing discoloration and deterioration of the composition and consequently improving the stability of the composition for disinfection or cleaning according to the present invention.
Examples of such stabilizers include beta-cyclodextrin, citric acid, magnesium chloride, MgCl2, ethylene diamine tertracetic acid (EDTA salt), and dibutylhydroxy toluene (BHT) As a representative example.
Meanwhile, it is preferable that the antioxidant is added in an amount of 1.5 wt% or less based on the total weight of the composition for disinfection or cleaning, and the stabilizer is added in an amount of 1 wt% or less based on the total weight of the composition for disinfection or cleaning according to the present invention.
In this step, a natural preservative different from antioxidants and stabilizers may be further added. Such a natural preservative may be a herb or a herbal medicine extract having antimicrobial activity, which can be mixed with the green tea extract. The inulin component And ginseng containing a saponin component. These natural preservatives can further increase the shelf-life of a composition prepared by inhibiting the growth of microorganisms.
In this step, in order to have additional sterilization and cleaning ability in addition to the above-mentioned green tea extract as a main washing component, isopropyl alcohol, ethanol, n-propanol, t-butanol, 2-butanol, pentanol, At least one selected from the group consisting of iodine, can be further mixed.
In this step, it is possible to further improve the physical properties of the composition prepared by further mixing the porous beads with the green tea extract.
Since the porous beads are typical examples of the chitosan, the low molecular weight chitosan has antimicrobial and antifungal activity as a natural material. Therefore, the porous beads can act as a preservative in the prepared composition and can further increase storage stability. And exhibits excellent biocompatibility so as to maintain skin moisturization when using the composition for disinfection or cleansing.
In this step, fragrance may be applied to the composition for disinfection or cleaning, which is prepared by mixing a natural fragrance with a green tea extract.
Examples of the natural fragrances include rosemary, lavender, rose, lavandin, lemon, rosewood, mandarin, jasmine, basil, ) Obtained from plants such as, for example, tomatoes, alfalfa, marigold, sandalwood, bergamot, citronella, orange, eucalyptus, chamomile, palmarosa or majoram. Extracts or essential oils may be used.
These natural fragrances each have antimicrobial activity on specific microorganisms, and when added for fragrance application, they exhibit the effect of simultaneously elevating the antimicrobial activity on the prepared disinfecting or cleaning composition, and can be preferably used.
In addition, the natural additives to be mixed in this step may be further constituted to further improve the physical properties of the composition for disinfection or cleaning which is prepared by further including a natural thickening agent.
At this time, the natural thickening agent is added for the stability of the bubbles that can be generated in the manufactured cleaning or disinfecting composition. In order to perform such a function, natural thickening agents such as natural starch such as starch, xanthan gum, locust bean gum, carrageenan, pectin gelatin, It is preferable to mix the thickening agent in an amount of 0.0001 to 10% by weight based on the total weight of the prepared composition for cleaning or disinfecting. If it is less than 0.0001% by weight, If the weight percentage is exceeded, the increasing agent is unstably dispersed in the disinfecting or cleaning composition.
The step (c) is a step of preparing a composition for disinfection or cleaning by adjusting the pH of the green tea mixture. The composition for disinfection or cleaning may be prepared by varying the pH depending on the application, As a control substance, it is possible to use a fruit acid such as grape seed extract, lemon juice, vinegar, and malic acid, or to lower the pH by using citric acid or the like, and to increase the pH by using potassium hydroxide or sodium hydroxide.
At this time, it is preferable that the pH of the natural pH adjusting substance to be added is adjusted by adding 0.01 to 0.5% by weight based on the total weight of the composition. If the pH is less than 0.01% by weight, In contrast, when the amount of the surfactant is more than 0.5% by weight, the acidity or basicity of the composition is excessively high, thereby deteriorating the function of the composition. Therefore, It is preferable to have a weak acidity of more than 5 and less than 7 so as to be able to maintain the acid value.
The composition for disinfection or cleaning prepared by the method for producing a composition for disinfection or cleaning according to the present invention as described above can be prepared in the form of a liquid, a gel, an emulsion or an aerosol, and a natural addition product And further adding a natural oil to the mixture to react with potassium hydroxide to form a soap and a natural glycerin. In this case, To produce a solid form.
The disinfection of a hospital room, which is treating patients with viral diseases, and the disinfection of the hands, the human body, clothing and medical devices of health workers are not straightforward. Generally, disinfecting using strong acids, strong bases or chlorine disinfectants, the aforementioned disinfectants effectively kill the virus, but are limited in practical use.
The use of the composition prepared by the method for manufacturing a composition for disinfection or cleaning according to the present invention as a disinfectant makes it possible to disinfect an animal or disinfect the animals, farmers and farmers directly.
When the composition prepared by the method for producing a composition for disinfection or cleaning according to the present invention is used, it can be used for direct disinfection or cleaning since it is free or weak in human health or environment, It can remove more than 90%, exhibits antimicrobial activity, effectively removes bacteria and harmful substances, and has antiviral activity even after prolonged time including antioxidant, so that various types of natural detergents and disinfectants are manufactured . ≪ / RTI >
Hereinafter, the present invention will be described in more detail with reference to preferred embodiments.
The embodiments presented are only a concrete example of the present invention and are not provided for the purpose of limiting the scope of the present invention.
≪ Example 1 >
In order to prepare the composition for cleaning or disinfecting according to the present invention of Example 1, a green tea extract was prepared using green tea leaves, green tea stems, and green tea seeds.
To prepare green tea extract, hot water extraction method was used. The green tea leaves, green tea stalks, and green tea seeds were mixed with steam and cooled. The thus-melted green tea was dried in a container heated at 200 to 300 캜, dried, (W / v) of the green tea powder was immersed in water at 70 to 90 ° C and allowed to stand for 12 to 24 hours. Then, the green tea extract was filtered and concentrated under reduced pressure to give green tea The extract was prepared.
1 to 10 parts by weight of ascorbic acid as an antioxidant was added to the green tea extract to prepare a cleaning or disinfecting composition.
<Experimental Example 1>
In Experimental Example 1, plaque formation inhibition experiments were conducted to examine the effect of green tea extract on the activity of influenza virus.
To this end, MDCK (Madin-Darby, canine kidney, ATCC, USA) was added to the MEM medium (Menimum Essential Medium: GIBCO Invitrogen Coporation, USA) containing Fetal Bovine Serum (FBS: GIBCO Invitrogen Coporation, USA) USA) were inoculated at a concentration of 1.5 × 10 5 cells / ml and cultured in a 6-well plate at 37 ° C. in a 5% CO 2 incubator.
A / Korea / 01/09 (H1N1) and A / Aquatic Bird / w81 / 05 (H5N2) were infected with 200 PFU (Plaque Forming Unit) in the MDCK cell line ) / Ml. ≪ / RTI >
After the infection treatment, the virus solution treated with cells was removed and the cell line was washed with 1 ml / well of PBS (Photometric Phosphate Bufferd Saline) buffer.
The green tea extract was diluted twice to a final concentration of 100 μg / mL to 6.25 μg / mL, and then diluted with an overlay medium (1% agarose, DMEM containing trypsin enzyme at a concentration of 10 μg / mL Modified Eagle's Medium: GIBCO Invitrogen Coporation, USA) medium], and an overlay medium was used as a control. The overlay medium was applied to the cells infected with influenza virus at 3 ml / well. After 15 minutes, the overlay medium was solidified and incubated for 72 hours in an incubator maintained at 37 ° C and 5% CO 2 .
As a result, the plaque was formed during the 72 hours, and plaque formation was inhibited in the experimental group to which the green tea extract of the present invention was added. 2, which is a photograph showing the results of the plaque inhibition experiment according to Experimental Example 1, it was confirmed that the plaque formation of the influenza virus was inhibited when treated with EGCG, which is the main component of the green tea extract, As the concentration of EGCG used increased, plaque formation was inhibited. However, it was confirmed that plaque formation was not inhibited in the case of the control group.
After each number of plaques was repaired by the plaque inhibition experiment, plaque formation inhibition ability of the influenza virus by the green tea extract was determined by the ratio of the plaque number of the control group and the plaque number of the test group.
<Experimental Example 2>
In the present Experimental Example 2 In order to confirm the virus removal capability of the green tea extract artificial skin cells, neo bonus ® E was added MEM medium according to the manufacturer's protocol using the (Tego Science, South Korea) and 24 hours at 37 ℃ CO 2 incubator After incubation, low-temperature adaptive X-31 virus was spotted by 5 x 10 4 PFU, washed with green tea extract, and the virus removal / inactivation effect by this washing effect was verified by plaque assay in MDCK cell line. More specifically, the cells were incubated and then washed with phosphate buffer saline (PBS) and the virus was spotted by 5 x 10 4 PFU. Then, the cells were cultured in a 37 ° C CO 2 incubator for 24 hours and washed with 100 μg / mL and 200 μg / mL of green tea extract or virus-infected artificial cells in PBS, respectively. For reference, the virus titer was confirmed by the plaque formation experiment performed in Example 1 with the washing solution to confirm the virus removal ability of the green tea extract.
Fig. 2 is a graph showing the result of the plaque formation experiment of Experimental Example 2. Fig.
As shown in FIG. 2, plaque was not formed in the virus-infected artificial cells in the medium washed with the green tea extract, and it was confirmed that the green tea extract had an excellent effect on the virus removal.
<Experimental Example 3>
In Experimental Example 3, ascorbic acid, an antioxidant, was added to green tea extract to increase the shelf life of green tea extract for use as a hand cleaner or a disinfectant. The antiviral activity of green tea extract was increased or decreased.
To this end, various concentrations of ascorbic acid were added to 100 ㎍ / mL of green tea extract and stored at room temperature for 2 months. At this time, plaque formation inhibition experiment was performed on 200 PFU of A / Korea / 01/09 (H1N1) virus once every 5 days, and the change of the shelf life of green tea extract was confirmed.
To confirm the increase of shelf life of antioxidants and stabilizers, stabilizers were added to various concentrations of green tea extract containing ascorbic acid and stored at room temperature for 2 months. In addition, a plaque formation inhibition experiment with 200 PFU of A / Korea / 01/09 (H1N1) virus was conducted once every five days to confirm the change in the shelf life of green tea extract by antioxidants and stabilizers.
As a result of measuring the change of the shelf life of green tea extract, the shelf life of the green tea extract was increased and it was confirmed that the antiviral activity of the green tea extract was maintained even after more than 2 months.
Claims (9)
(b) adding an antioxidant and a stabilizer to the green tea extract to prepare a green tea mixture; And
(c) adjusting the pH of the green tea mixture to prepare a composition for cleaning or disinfection.
Wherein the step (a) comprises the steps of:
1) Collecting green tea leaves, green tea stalks and green tea seeds and boiling them with steam;
2) drying the green tea leaves, green tea stalks and green tea seeds, which have been matured in step 1), followed by drying;
3) preparing green tea powder by pulverizing the green tea leaves, green tea stalks and green tea seeds dried in step 2);
4) hot water extraction of the green tea powder to prepare a hot water extract; And
5) The hot-water extract is filtered and concentrated to obtain a green tea extract.
The green tea extract contains at least one of catechin, epicatechin, epigallocatechin (EGC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG) and flavonoid ) Derivative, and the composition is contained in an amount of 20% or less based on the total weight of the composition.
Wherein the antioxidant is at least one compound selected from the group consisting of tocopherol, ascorbic acid, ascorbic acid derivatives, gallic acid, quercetin, flavonol, flavonoid, catechol, ethoxyquin and lignan glycosides, % Or less of the total weight of the composition.
The stabilizer may be selected from the group consisting of? -Cyclodextrin, citric acid, magnesium chloride, MgCl2, ethylenediaminetetraacetic acid (EDTA salt), and dibutylhydroxy toluene (BHT) Wherein the composition is contained in an amount of 1% or less based on the total weight of the composition.
In the step (b), at least one selected from the group consisting of isopropyl alcohol, ethanol, n-propanol, t-butanol, 2-butanol, pentanol, hexanol and povidone- By weight based on the total weight of the composition.
Wherein the porous beads, the natural fragrance or the natural additives are further mixed in the step (b).
Wherein the composition for cleaning or disinfecting has antiviral activity against enveloped virus.
Wherein the coating virus is an influenza virus, a retrovirus, an epstein-barr virus or a herpes simplex virus.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020140190860A KR20160079473A (en) | 2014-12-26 | 2014-12-26 | Preparation method of cleaning or disinfecting composition having antiviral activity by containing green tea extract as active component |
| PCT/KR2015/012801 WO2016104967A1 (en) | 2014-12-26 | 2015-11-26 | Cleansing or disinfecting composition having anti-viral activity by comprising green tea extract as active ingredient |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| KR1020140190860A KR20160079473A (en) | 2014-12-26 | 2014-12-26 | Preparation method of cleaning or disinfecting composition having antiviral activity by containing green tea extract as active component |
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| KR20160079473A true KR20160079473A (en) | 2016-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| KR1020140190860A KR20160079473A (en) | 2014-12-26 | 2014-12-26 | Preparation method of cleaning or disinfecting composition having antiviral activity by containing green tea extract as active component |
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| Country | Link |
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| KR (1) | KR20160079473A (en) |
| WO (1) | WO2016104967A1 (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106265381A (en) * | 2016-10-14 | 2017-01-04 | 柳州立洁科技有限公司 | A kind of moist type handwashing liquid |
| WO2018212428A1 (en) * | 2017-05-17 | 2018-11-22 | (주)하이씨엔티 | Hydrogen soap and manufacturing method therefor |
| KR20190040560A (en) * | 2017-10-11 | 2019-04-19 | 농업회사법인 주식회사 하이그린팜 | Manufacturing method for hydrogen powder soap |
| KR20210108805A (en) | 2020-02-26 | 2021-09-03 | 심승규 | Body-wearing-Goods having anti-virus and anti-bacterial effects, for example a mask, clothes, etc |
| KR20210108807A (en) | 2020-02-26 | 2021-09-03 | 심승규 | Cosmetics having anti-virus and anti-bacterial effects, for example lotion etc |
| KR102298942B1 (en) * | 2021-05-13 | 2021-09-07 | 주식회사 기본에 | Wet wipes composition for dish cloth having excellent sterilization and deodorizing effect and wet wipes for dish cloth including the same |
| KR20220019550A (en) * | 2020-08-10 | 2022-02-17 | 한상진 | Car cleaner manufactured by the nature enzyme |
| KR20230106798A (en) | 2022-01-07 | 2023-07-14 | 김종달 | Hand sanitizer using Selaginella tamariscina and manufacturing method thereof |
| KR102575807B1 (en) * | 2023-04-19 | 2023-09-07 | 한려이엠에스농업회사법인주식회사 | The manufacturing method and composition of eco-friendly detergent using spinach, green tea, oyster shell and resin |
| KR20230149132A (en) | 2022-04-19 | 2023-10-26 | 고려대학교 산학협력단 | Antiviral composition for Epstein-Barr virus comprising catechol compound or derivative thereof as an active ingredient |
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| US20220409653A1 (en) * | 2020-05-02 | 2022-12-29 | Viron, Inc. | Method for preventing entry and replication of enveloped viruses |
| WO2023027939A1 (en) * | 2021-08-22 | 2023-03-02 | Viron, Inc. | Method for preventing entry and replication of enveloped viruses |
| CN113930974A (en) * | 2021-11-05 | 2022-01-14 | 高梵(浙江)信息技术有限公司 | Processing method and equipment of plant fragrance antibacterial down feather |
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| KR20050001737A (en) | 2003-06-26 | 2005-01-07 | 종근당건강 주식회사 | A anti-inflammatory/abirritant composition for cleaning a anus, extracted from mixture of mugwort, purslane and green tea plant |
| KR20050054532A (en) | 2003-12-05 | 2005-06-10 | 염재한 | A washing water of green tea |
| KR101060794B1 (en) | 2005-02-23 | 2011-08-30 | 주식회사 엘지생활건강 | Antiviral agent against influenza virus consisting of plant extracts and hygiene products containing same |
| KR101196113B1 (en) | 2010-12-07 | 2012-10-30 | 전라남도 | Method for manufacturing HandWash and HandWash composition Thereof containing natural oriental plant extracts |
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| JP2727471B2 (en) * | 1989-09-14 | 1998-03-11 | 三井農林株式会社 | Influenza virus infection prevention agent |
| KR20000002239A (en) * | 1998-06-18 | 2000-01-15 | 성재갑 | Bactericidal deodorizing detergent composition |
| JP5346454B2 (en) * | 2007-09-21 | 2013-11-20 | 花王株式会社 | Antibacterial detergent composition |
| KR100961879B1 (en) * | 2009-10-27 | 2010-06-09 | 한국식품연구원 | Alcoholic disinfectant containing green tea extract |
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- 2014-12-26 KR KR1020140190860A patent/KR20160079473A/en not_active Application Discontinuation
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20050001737A (en) | 2003-06-26 | 2005-01-07 | 종근당건강 주식회사 | A anti-inflammatory/abirritant composition for cleaning a anus, extracted from mixture of mugwort, purslane and green tea plant |
| KR20050054532A (en) | 2003-12-05 | 2005-06-10 | 염재한 | A washing water of green tea |
| KR101060794B1 (en) | 2005-02-23 | 2011-08-30 | 주식회사 엘지생활건강 | Antiviral agent against influenza virus consisting of plant extracts and hygiene products containing same |
| KR101196113B1 (en) | 2010-12-07 | 2012-10-30 | 전라남도 | Method for manufacturing HandWash and HandWash composition Thereof containing natural oriental plant extracts |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106265381A (en) * | 2016-10-14 | 2017-01-04 | 柳州立洁科技有限公司 | A kind of moist type handwashing liquid |
| WO2018212428A1 (en) * | 2017-05-17 | 2018-11-22 | (주)하이씨엔티 | Hydrogen soap and manufacturing method therefor |
| KR20190040560A (en) * | 2017-10-11 | 2019-04-19 | 농업회사법인 주식회사 하이그린팜 | Manufacturing method for hydrogen powder soap |
| KR20210108805A (en) | 2020-02-26 | 2021-09-03 | 심승규 | Body-wearing-Goods having anti-virus and anti-bacterial effects, for example a mask, clothes, etc |
| KR20210108807A (en) | 2020-02-26 | 2021-09-03 | 심승규 | Cosmetics having anti-virus and anti-bacterial effects, for example lotion etc |
| KR20220019550A (en) * | 2020-08-10 | 2022-02-17 | 한상진 | Car cleaner manufactured by the nature enzyme |
| KR102298942B1 (en) * | 2021-05-13 | 2021-09-07 | 주식회사 기본에 | Wet wipes composition for dish cloth having excellent sterilization and deodorizing effect and wet wipes for dish cloth including the same |
| KR20230106798A (en) | 2022-01-07 | 2023-07-14 | 김종달 | Hand sanitizer using Selaginella tamariscina and manufacturing method thereof |
| KR20230149132A (en) | 2022-04-19 | 2023-10-26 | 고려대학교 산학협력단 | Antiviral composition for Epstein-Barr virus comprising catechol compound or derivative thereof as an active ingredient |
| KR102575807B1 (en) * | 2023-04-19 | 2023-09-07 | 한려이엠에스농업회사법인주식회사 | The manufacturing method and composition of eco-friendly detergent using spinach, green tea, oyster shell and resin |
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| Publication number | Publication date |
|---|---|
| WO2016104967A1 (en) | 2016-06-30 |
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