KR101025330B1 - Soap composition comprising marine deep sea water and marine collagen - Google Patents

Abstract

The present invention relates to a soap containing deep sea water and marine collagen, and more particularly, to a soap composition comprising a caustic soda-deep sea water solution and marine collagen dissolved in a deep sea water of base oil, caustic soda, and a soap using the same. It relates to a manufacturing method.

Description

Soap composition containing deep sea water and marine collagen {SOAP COMPOSITION COMPRISING MARINE DEEP SEA WATER AND MARINE COLLAGEN}

The present invention relates to a soap comprising deep sea water and marine collagen, and more particularly, to a soap composition comprising a base oil, a caustic soda solution containing deep sea water and marine collagen, and a method of preparing a soap using the same. .

Skin is the largest organ of our body and occupies about 16% of our body volume. It plays an important role in protecting human body from external stimuli such as temperature, humidity and UV rays and harmful microorganisms. However, as we age, our skin is damaged by natural aging, various contaminants, strong UV rays, stress and nutritional deficiencies, which can cause wrinkles, dryness, Skin aging may occur, such as loss of elasticity or keratinization.

In addition, the decrease in the moisturizing power of the skin, the slowing of the skin keratinization rate is also a phenomenon due to skin aging. That is, as aging progresses, the amount of collagen, mucopolysaccharide, and hyaluronic acid in the dermis matrix, which plays an important role in maintaining skin elasticity, decreases and the amount of insoluble collagen increases. The dermis becomes difficult to keep the skin hydrated, and the skin becomes rough. In addition, the function of sebaceous glands and sweat glands is reduced, and the amount of sebum and sweat is gradually reduced, and sebaceous membranes are not properly formed, and thus the protection function for skin moisturization is lost, and thus, the skin becomes dry and translucence. Will lose skin elasticity and wrinkles. Loss of moisturizing of the skin is a major symptom of aging along with decreased skin elasticity and wrinkle formation and is closely related.

On the other hand, soap is a broad term means saturated and unsaturated higher fatty acids, resin acids, naphthenic acid metal salts collectively, narrow meaning is the higher fatty acids mainly used for washing refers to alkali metal salts. In kind, strong soaps such as sodium salts of higher fatty acids (eg sodium stearate), sweet soaps such as potassium salts (eg potassium stearate), liquid soaps such as ammonium salts (eg ammonium stearate), other metal salts are metal soaps. Etc. The molecule of the soap consists of a hydrophobic portion consisting of a long ring of hydrocarbons (16 to 22 carbon atoms) and a hydrophilic portion in which a carboxyl group is bonded to an alkali metal (Na ⁺ , Ca ²⁺ , NH ^4+, etc.). Soap is an anion (OH ^-) in the water and the cations (H ⁺⁾ is digested with the surface tension of water, lower the surface tension and foaming power, has an emulsion capacity, the available capacity powerful cleaning action.

It is made by mixing most of the soaps currently on the market with alkali metals and chemicals. When used to clean the skin, aqueous solution shows alkalinity, excessive sebum of skin is removed, softening the stratum corneum and drying skin And problems such as causing roughness or irritating the skin often occur.

Therefore, there is a need for the development of a soap that is hypoallergenic without causing skin dryness and roughness, and which can prevent skin aging and impart elasticity.

In order to meet the above demands, an object of the present invention is to provide a soap that is hypoallergenic and has excellent skin moisturizing and elasticity-producing effects and a method for producing the same.

The present invention provides a soap composition containing a base oil, caustic soda-ocean deep water solution and marine collagen dissolved in deep sea water.

In another aspect, the present invention provides a soap prepared from the soap composition.

In another aspect, the present invention provides a soap manufacturing method comprising the step of adding a caustic soda-ocean deep water solution dissolved caustic soda in deep sea water to the base oil, and the addition of marine collagen.

The manufacturing method

Dissolving caustic soda in deep sea water to produce a caustic soda-sea deep water solution;

Mixing and stirring the caustic soda-ocean deep water solution and base oil; And

Adding marine collagen to the mixture

It may be to include.

In addition, the manufacturing method may further comprise the step of additionally adding additives such as essence oil and natural preservatives. In addition, depending on the final soap form, it may further comprise a commercialization step such as molding, aging and drying the soap, liquefying, powdering, or filming.

The present inventors manufacture soap using deep sea water, rich in minerals, instead of ordinary water such as general water, distilled water, and disinfectant water, which are generally used for soap production. A high-performance soap with hypoallergenic, stable, absorbent, and moisturizing marine collagen ingredients that is safe against atopic or sensitive skin as well as antioxidant, immunity, skin elasticity and anti-wrinkle effects. The present invention was completed by discovering that it can be prepared.

Hereinafter, the present invention will be described in more detail.

The present invention is characterized in that soap is prepared from a caustic soda-sea deep water solution in which caustic soda is dissolved in deep sea water rather than ordinary water.

The deep sea water generally refers to seawater resources that exist at a depth of 200 m or more, where sunlight does not reach, and since sunlight does not reach, photosynthesis does not occur, and organic matter and pollutants do not flow from land, In comparison, it is kept pure and clean. In addition, the water temperature is constant at about 2 ° C, and is rich in useful substances including various mineral components such as calcium, potassium, magnesium, and essential trace elements.

Deep water cleanliness is environmentally friendly due to less harmful chemicals and organic matters that need to be decomposed, prevents secondary bacterial contamination, and has few impurities, which is effective for sensitive skin such as atopic skin. In addition, low temperature is stable at low temperature due to low water temperature fluctuations and rich in minerals, various essential minerals are present, showing the amazing effect of eliminating active oxygen by the action of metal ions. In addition, deep sea water is matured over a long period of time under high pressure, and the aggregates of water molecules are small groups, so that the surface tension is small, so that the permeability is high and the thermal conductivity is high. Due to these characteristics, the use of deep sea water can promote the blood circulation of the skin, thereby making the metabolism of the skin active.

Deep sea water used in the present invention is desalted to a hardness of 100 to 200 ppm, preferably 120 to 180 ppm, more preferably 140 to 160 ppm. If the hardness of the deep sea water is lower than the above range, it is difficult to obtain a beneficial effect because the content of mineral components is insignificant, and if it is higher than the above range, the water of the deep sea water has a high mineral content, but the cleaning power is deteriorated and it may cause skin irritation. It is good to do.

In order to obtain the deep sea water in the preferred hardness range as described above, desalination of the collected deep sea water is carried out. The desalting process may be performed by all conventional seawater desalination processes, for example, reverse osmosis membrane filtration and electrodialysis. , Ion exchange method, or freshwater dilution method.

In an embodiment of the present invention, it can be used by desalting so as to have a hardness in the above range that is collected at a depth of about 550 to 750 m off the Ulleungdo offshore.

Caustic soda-ocean deep water solution used for the soap production in the present invention is 1: 1.5 to 3, preferably 1: 1.7 to 2.5, more preferably based on caustic soda and desalted seawater in the hardness range as described above Preferably, the solution is obtained by mixing at a mixing ratio of 1: 2 to 2.5 (caustic soda weight: weight of deep sea water). If the ratio of the depth of the deep sea water to the caustic soda is lower than the above range, the desired effect of various minerals may not be obtained, and if it is higher than the above range, the mixing ratio of the caustic soda and the deep sea water may be an obstacle to the soap production. It is good to set it as the above range.

As the base oil used in the soap production of the present invention, all kinds of oils usable for the production of a conventional soap may be used. For example, as the base oil, one kind selected from the group consisting of coconut oil, cottonseed oil, palm oil, castor oil, olive oil, sweet almond oil, grape seed oil, jojoba oil, sunflower seed oil, camellia oil, avocado oil, calendura oil, rice bran oil, etc. The above can be used. The coconut oil (Coconut oil) is an oil obtained from copra, which is a dry pulp of coconut, is an excellent moisturizing ingredient, and makes soap bubbles well. Olive oil has excellent moisturizing effect and softens soap. Palm or cottonseed oils make soap bubbles rich and fast and harden the soap itself. Grapeseed oil is an oil derived from grape seeds that is rich in vitamin C to prevent oxidation of other oils. It also helps to hydrate skin with antioxidant activity. Sweet almond oil is characterized by its softening, moisturizing and penetrating properties. Jojoba oil and camellia oil are similar to sebum in human body and are good for atopic skin or dry and sensitive skin. Sunflowerseed oil and avocado oil are linolenic acid and lecithin. And vitamin-A, D, E is rich in skin tissue regeneration and skin soothing effect. In addition, calendula oil has an anti-inflammatory or softening effect, and rice bran oil (Rise Bran oil) is rich in vitamin E and minerals and has a good moisturizing effect.

In a preferred embodiment of the present invention, the base oil is 27 to 33 parts by weight of coconut oil, 27 to 33 parts by weight of palm oil, 13 to 17 parts by weight of castor oil, olive oil 9 with respect to a total of 100 parts by weight (total base oil weight) To 13 parts by weight, sweet almond oil 9 to 13 parts by weight, and grape seed oil may be to include 3 to 7 parts by weight.

The base oil and the caustic soda-ocean deep water solution are mixed to prepare a soap, wherein the mixing ratio of the base oil and the caustic soda-ocean deep water solution is 1: 0.1 to 0.8, preferably 1: 0.2 by weight. To 0.7, more preferably 1: 0.3 to 0.6 (base oil weight: caustic soda-ocean deep water solution weight). If the content of the caustic soda-ocean deep water solution for the base oil is less than the above range, it is difficult to produce soap, and if it is more than the above range, the irritation becomes stronger and the cleaning power is lowered.

When mixing the base oil and the caustic soda-ocean water solution, the base oil is warmed to 45-65 ° C., preferably 50-60 ° C., in order to facilitate easy and uniform mixing of the material and soap making. It is preferable to mix the caustic soda-ocean deep water solution at a temperature of from -60 [deg.] C., preferably 50-55 [deg.] C., and stir for about 5 to 30 minutes, preferably about 10 to 20 minutes.

Another feature of the soap according to the invention is that it contains marine collagen. Collagen previously contained in cosmetics and foods are animal collagen extracted from bones and skins such as pigs and cattle, and various problems have been derived according to mad cow disease, foot and mouth disease, and have an autoimmune disease or allergic reaction to animal collagen. People are not allowed to use, and developed countries and Korea also ban the use of collagen extracted from terrestrial animals. Accordingly, recently, vegetable collagen has been used. Since vegetable collagen itself is different from the human body, the protein collagen has low water absorption when applied to the skin, and is significantly lower than animal collagen in terms of efficacy and effectiveness. Have a report.

Thus, the present invention is characterized by using marine collagen extracted from safe and effective natural marine raw materials. Marine collagen extracted from marine organisms has a structure similar to that of animal collagen, and has a very low risk of contamination due to transmission, in particular, an infectious pathogen of a prion type, and its amino acid composition is relatively different from that of human collagen. Since it is relatively easily distinguishable by an antibody, it can play a very effective immunological label when applied to a human body. In addition, it has excellent cell affinity, low cytotoxicity, low risk of immune response, and is suitable for skin, so it has excellent skin absorption.

Marine collagen is a hydrolyzate obtained by treating proteolytic enzymes such as trypsin, and because it is low molecular weight by enzymatic treatment, it is quick to absorb the body or skin, is convenient to use in liquid form, and the content of serine is high. It is high in skin wrinkle improvement, and proline contains moisturizing function. In addition, the marine collagen has a high thermal denaturation temperature, so that even when the skin is applied, the triple helix structure of the collagen is excellent in stability, and is most effective among proteins used in cosmetics, pharmaceuticals, and foods. In addition, marine collagen is easily absorbed at human body temperature (36.5 ° C.) because the gelation temperature is 30 ° C. or lower, but animal collagen is solid at body temperature because the gelation temperature is 40.1 ° C., so the absorption rate is low. Animal collagen has a molecular weight of 5000-6000, but the marine collagen molecular weight is about 500 to 1500 is a low molecular protein. Therefore, marine collagen is advantageous in terms of absorption rate and ease of use than general land animal collagen extracted from bones or skins of cows, pigs, chickens, and the like.

Marine collagen used in the present invention is obtained by treating a marine organism such as fish scales and / or sponges with a hydrolytic enzyme such as trypsin, and has a gelation temperature of 15 to 30 ° C., preferably 15 to 25 ° C. The average molecular weight is preferably about 500 to 1500, preferably about 1000 to 1500. The fish scales are not particularly limited, but may be used for tibia fish, especially dome scales.

In particular, marine collagen is four times higher than other collagens in the amount of serine in the amino acid, which improves wrinkles on the skin, and is rich in proline, which has a skin repair and moisturizing function. In the present invention, the content of the marine collagen is 0.01 to 1% by weight, preferably 0.05 to 0.5% by weight, more preferably based on the total weight of the soap composition (including base oil, caustic soda-sea deep water solution and marine collagen) Preferably from 0.07 to 0.2% by weight. If the content of marine collagen is less than the above range, the desired effect of the desired marine collagen cannot be sufficiently obtained. If the content of the marine collagen is more than the above range, the effect of the content of marine collagen is insignificant and the absorption rate is lowered. It is good to make it a range.

Marine collagen in the present invention is excellent in skin affinity and stability, suitable for all skin types, and the skin surface is firm because it has an excellent ability to attract moisture in the air from the skin surface and keep it on the skin for a long time. Gives wrinkles to relieve the skin, elasticity of the skin surface, and has an antioxidant and immune boosting effect.

Another feature of the present invention is that it further contains an algae extract inhabiting the ocean can obtain additional immunity enhancing effect, antibacterial and deodorant effect. Seaweed extracts which may be further included in the soap composition according to the present invention are not particularly limited, and include, for example, Ecklonia cava , rhubarb (sea oak, Eisenia bicyclis ), seaweed ( Undaria pinnatifida ), kelp ( Laminaria japonica), One seaweed selected from the group consisting of Sargassum fulvellum , vel ( Hizikia fusiformis ), laver ( Porphyra tenera ), grazing ( Gracilaria verrucosa ), auditory (Codium fragile ) and green seaweed ( Ulva lactuca ) At least one solvent selected from the group consisting of water, desalted deep sea water, C1-5 low-cost alcohols, hexane, chloroform, and acetone, at least 10 times, preferably 20 to 50 times, the weight of the powder. More preferably, the extract may be obtained by using 30 to 45 times. In particular, when the desalted deep seawater is used alone or in combination with other solvents as an extraction solvent, it is preferable to create an appropriate osmotic environment in addition to the extraction action and to synergize with the seaweed extract components. The desalted deep sea water may be desalted to a hardness of 100 to 200 ppm, preferably 120 to 180 ppm, more preferably 140 to 160 ppm. The extraction method is not particularly limited, and all methods commonly used for plant extraction may be used, and for example, reflux extraction, hot water extraction, or high pressure extraction may be used.

In the present invention, the amount of the seaweed extract added is 0.001 to 0.1% by weight, preferably 0.01 to 0.05% by weight, more preferably 0.01 to 0.02% by weight based on the total weight of the final soap composition including the seaweed extract. It is good to be%. If the content of the seaweed extract is less than the above range, the desired effect cannot be sufficiently obtained, and if the content of the seaweed extract is more than the above range, the effect of the algae extract in the soap composition of the present invention is preferably within the above range.

Ecklonia cava is a perennial brown algae, distributed in the southern coast of Korea, near Jeju Island, and Japan. Among the algae, brown algae are excellent in nutritional and symbolic aspects compared to other algae and have abundant yields, which are widely used for food and seaweed resources. Ecklonia cava extract has been used in functional foods and feeds as it has anti-oxidant, antibacterial, bad breath suppression, anti-cancer, immunity, whitening, and metalloproteinase inhibitory activity with allergic diseases. Rhubarb (Sea oak, Eisenia bicyclis ) is a kind of brown seaweed seaweed and is also used as a substitute for kelp with gompi or Ecklonia cava. It is mainly distributed in Korea (Ulleungdo, Dokdo) and Japan, and lives in the lower intertidal rocks within 10m of water depth. It is also used as a raw material for alginic acid and contains a large amount of minerals such as iodine and potassium. Rhubarb components have been reported to be effective in boosting immunity, antibacterial, bad breath, and anticancer effects.

 The soap composition according to the present invention may further contain additives in addition to the base oil, caustic soda-ocean deep water solution, and marine collagen as described above. The amount of the additive may be appropriately adjusted according to the final soap type, use, and the like, and may generally be about 0.01 to 3 wt% based on the total weight of the soap composition. The additives may be conventional ingredients usable for cleaning or skin improvement purposes, for example essence oils, natural preservatives and the like. The essence oil is a natural essence oil, neroli (neoli) having a bactericidal, cell regeneration effect and delayed skin aging effect, exfoliation and inflammatory skin disease improvement effect (lemon) and blemish improvement effect and skin regeneration effect Sweet orange, Lavender & Cypress for oily or acne skin, Jasmine to improve sexual disorders, Tea tree with antibacterial and antifungal effects, Good for eczema or blood circulation Pine, Ylangylang, which has anti-inflammatory or hair growth effects, Grapefruit, which promotes lipolysis, disinfection and digestion, Rose and eye recovery for stress relief or pleasure It may be one or more selected from the group consisting of rosemary, etc., which have a soothing effect on the skin, and the amount thereof is sufficient to enhance the effectiveness of the essence oil. It may be 0.005 to 2.5% by weight based on the total weight of the soap composition in consideration of the over-exercise and the relationship with other components. In addition, the natural preservative may be one or more selected from the group consisting of tocopherol, grapefruit seed extract, rose marioyl extract, citric acid, etc., may be 0.005 to 1% by weight based on the total weight of the soap composition. In addition to this, natural flavors, natural pigments and the like commonly used in soap production may be further included in an appropriate amount.

Soaps prepared from the soap compositions of the present invention may be commercialized in various forms, such as solids, liquids, films, powders, and the like, particularly when they are solid, and the shaping and commercialization processes are typically in the art. Follow the known method. The soap manufacturing process of the present invention is illustrated in FIG.

Soap according to the present invention contains natural materials such as vegetable base oil, natural essence oil, deep sea water, marine collagen and the like, glycerin is naturally produced during the soap aging process is a good moisturizing product. In addition, since it does not use artificial chemical dyes, chemical fragrances, chemical surfactants, chemical preservatives, and chemical curing agents, it is an environmentally friendly product that helps skin health and the natural environment by minimizing skin irritation.

The soap composition according to the present invention and the soap prepared therefrom contain marine deep water mineral component and marine collagen component excellent in hypoallergenicity, stability, absorbency and moisturization extracted from marine life, and optionally specific seaweed extract, thereby providing excellent skin moisturization. It exhibits antioxidant power, immunity, skin elasticity and anti-wrinkle effect, and is characterized by providing excellent effects on atopic or sensitive skin.

Hereinafter, the present invention will be described in more detail with reference to specific examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Example 1 Preparation of Natural Soap Containing Deep Sea Water and Marine Collagen

Coconut oil, palm oil, pizza horse oil, olive oil, sweet almond oil, and grape seed oil supplied from Herb Nuri were warmed to 50 to 60 ° C. to prepare a base oil. Meanwhile, the deep sea water taken from 650m off the coast of the East Sea was desalted by Reverse Osmosis to make 150 ppm of hardness, and then dissolved caustic soda to adjust the temperature to 50-55 ℃. A deep sea water solution was prepared.

The base oil and the caustic soda-ocean deep water solution obtained above were mixed and stirred for about 15 minutes. Then, add marine collagen (Kumho Hwaseong Co., Ltd. and / or Herb Nuri Co., Ltd.) and essence oil (Neroli, Lemon and Sweet Orange, purchased from Herbnuri Co., Ltd.) and fill it with a molding mold for 37 to 42 hours. After warming to ℃, it was cut to about 90 to 100g size, aged and dried for 3 to 4 weeks to prepare a soap.

The components used in this example and their contents are shown in Table 1 below.

[Table 1] Addition amount of each ingredient based on 1kg soap

Example 2: Preparation of Natural Soaps Further Containing Seaweed Extracts

Soap was prepared in the same manner as in Example 1, except that the seaweed extract was added.

Seaweed extract used in this example was prepared as follows: Ecklonia cava (Donghae Fisheries Promotion Agency) and Rhubarb (Haewon Biotech Co., Ltd.) were washed several times with water to remove salt, and then frozen at -40 ° C or lower for 2 to 3 days. Dry or vacuum dried. The dried sample was pulverized using a pulverizer, and then fine powder was made using a crusher. Deep sea water: ethanol (1: 1), desalted at 150 ppm hardness, was added to the prepared powder in an amount of 40 times the weight of the powder, followed by stirring for 1 day, and the supernatant was separated using a centrifuge (5000 rpm 5). min). The separated extract was dried in a vacuum evaporator or a dryer at 60 to 65 ℃ to prepare a seaweed extract.

The seaweed extract thus prepared was added to the mixture of the base oil and the caustic soda-ocean deep water solution in the amounts shown in Table 2 below. The components and contents of this example are shown in Table 2 below.

[Table 2] Addition amount of each component based on 1kg soap

Example 3: efficacy test of seaweed extract

Efficacy and comparison of Ecklonia cava, rhubarb and wakame seaweed were analyzed for extraction of hot water using purified water and deep sea water. Sample pretreatment was carried out based on Figure 2, the efficacy verification was measured for general ingredient analysis, yield, polyphenol content, antioxidant activity, antibacterial experiment and whitening effect. Reagents used in all experiments were purchased from Sigma Aldrich (USA) and Merck (Germany) and used for analysis.

Ecklonia cava (Donghae Fisheries Promotion Agency), Rhubarb (Haewon Biotech Co., Ltd.) and Seaweed (Uljin Suhyup Market) were washed several times with water to remove salt, and then freeze-dried or vacuum dried at -40 ° C for 2 to 3 days. The algae dried in this way was pulverized using a pulverizer, and then fine powder was made using a rake. To each of the prepared seaweed powders, normal water (third distilled water) or deep seawater desalted at a hardness of 100 to 200 ppm was added in an amount of 40 times the weight of the powder and stirred for 1 day, followed by supernatant using a centrifuge. Isolated (5000 rpm 5 min). The separated extract was dried in a vacuum evaporator or a dryer at 60 to 65 ° C. to prepare purified water extract and deep sea water extract for each of the three seaweeds.

Efficacy verification was performed in the following manner:

① General ingredient analysis

Ash was carried out according to the general ingredient test method contained in the Food Code, moisture measurement was measured using a moisture meter (Sartorius, Germany). Crude fat was measured colorimetrically using crude fat analyzer (Gerhardt, Germany) and protein measurement using crude protein analyzer (Gerhardt, Germany).

② polyphenol compound

Example 3 Total polyphenolic compounds were measured using seaweed crude extracts using deep seawater according to the method. First, 0.5 ml of Folin-Ciocalteu reagent and 1 ml of 7.5% Na ₂ CO ₃ were added to 1 ml of crude extract, and the final 10 ml was adjusted with distilled water. After incubation for 20 minutes in the incubator (60 ℃) prepared in advance, the absorbance was measured at a wavelength of 765 nm. The polyphenol content in the sample was calculated from the calibration curve previously obtained using gallic acid.

③ Antioxidant Activity Test Method

The antioxidant activity assay measured the free radicals of DPPH (1,1-diphenyl-2-picrylhydrazyl).

As an electron scavenging method for DPPH free radicals, 0.8 ml of DPPH (16 mg / 100 ml EtOH) solution was mixed with 0.2 ml of the sample, reacted at room temperature for 10 minutes, and absorbance was measured at 517 nm. % Inhibition is compared with the control without the sample,

Percentage (%) = [(A ₀ -A ₁ ) / A ₀ ] × 100

(A ₀ = control absorbance A ₁ = experimental group absorbance)

As shown.

④ Antibacterial measurement

Bacillus subtilis, Escherichia coli and Staphylococcus aureus were used to add three bacteria, and 2% by weight of Ecklonia cava and rhubarb extracts were added. The OD (600 nm) was measured by spectrophotometer.

⑤ Determination of tyrosinase activity inhibition

Tyrosinase inhibitory activity measurement method was measured by the colorimetric method of DOPAchrome produced as a result of the action of tyrosinase. Sample in 0.2 ml of mushroom tyrosinase (100 unit / ml, Sigma USA), 0.2 ml of 1/15 M potassium phosphate buffer (pH 6.8), 0.4 ml of substrate 10 mM DOPA (DL-β-3,4-dihydroxyphenyl alanine) 0.2 ml of the solution was added and reacted at 25 ° C. for 15 minutes, measured at 475 nm, and the change in DOPAchrome was converted into inhibition rate (% = [(A ₀ -A ₁ ) / A ₀ ] × 100).

The results of the general component analysis in the seaweed extract obtained as a result of the above experiment are shown in Table 3 below, and the respective yields are shown in FIG. 3.

[Table 3]

(A: Seaweed Extract Using Deep Sea Water B: Seaweed Extract Using Normal Water)

As can be seen in Table 3 and Figure 3, the general composition and yield of the seaweed extract was not significantly different according to the extract used as a general purified water and deep sea water. However, there was a difference in polyphenol content. Figure 4 shows the results of the polyphenol measurement, it can be seen that the polyphenol content is increased more when extracted with deep sea water than when extracted with normal purified water.

The antioxidant test using the DPPH radicals is a method commonly used in aromatic compounds, in particular phenolic structure, to measure the free radical scavenging activity of DPPH reagent having free radicals in the molecule. The measurement results are shown in FIG. 5, and it can be seen that the antioxidant activity (DPPH radical scavenging activity) increases when extracted with deep sea water than when extracted with normal purified water.

The antimicrobial activity measurement results are shown in FIGS. 6 to 8. The antimicrobial activity was not significantly different depending on the type of fungus, but Staphylococcus aureus> Bacillus subtilis> Escherichia coli reacted more sensitively. Ecklonia cava extract showed better antimicrobial effect.

As a whitening efficacy test, tyrosinase inhibition was measured by an in vitro experiment as described above. DOPAchrome produced as a result of the action of tyrosinase was measured by colorimetry and the results are shown in FIG. 9. As shown in FIG. 9, the case of Ecklonia cava (28%) showed more whitening effect than rhubarb (12.8%) or wakame seaweed (13.1%), but there was no difference according to the extraction solvent.

 There was no significant difference in yield, general composition, and whitening effect when extracting hot water using algae as general purified water and deep sea water. However, when extracted with deep sea water, polyphenol content, antioxidant power, and antibacterial activity were better. Therefore, including seaweed extract using deep seawater may be a preferred example of the present invention. As a result, it is expected that seaweed extract using deep seawater has a variety of physiologically active effects when it is used in food, medicine, and cosmetic raw materials, rather than using extract using general purified water.

Example 4: Effect test on skin moisturizing and cleansing power

After using the natural soap prepared in Example 1 was allowed to wash twice a day to evaluate for each evaluation item once a week using for 1 month and summarized the results as follows.

The survey confirmed the following points.

① gender, name, age

[Table 4]

② skin moisture

③ amount of skin oil

④ Skin curvature

Items ② to ④ were obtained by recording the measured values of 10 steps by using a moisture meter. Table 5 below shows the evaluation criteria indicating the skin condition according to the measurement.

TABLE 5

-5 -4 -3 -2 -One 0 +1 +2 +3 +4 +5 Moisture measurement skin condition
(water%)
Very dry (<32) inattention
(32-33)
Normal (33-39) Wetting
(39-41)
High Moisture (＞ 41) Oil
Measures skin condition Ouch
inattention inattention normal Intelligence Go
Intelligence Skin flexion skin condition Rough skin normal Soft skin

 Soap A prepared in Example 1 and natural soap type B commercially available on the market (Herburi Co., Ltd. product: Soap made of Melt Pour, palm, coconut, castor, grape seed, olive (pure) , Shea butter, avocado, jojoba, ethanol, caustic soda, distilled water glycerin, product made by adding essence oil) was used for one month, and then the items ② to ④ were evaluated four times every seven days. The experiment was conducted for two months and randomly extracted and measured in 30 subjects regardless of gender. The average scores are shown in Table 6 and Table 7 below.

TABLE 6

Period (Type A) 1 week 2 weeks 3 weeks 4 Weeks Measurement area ball nose ball nose ball nose ball nose Skin moisture -2.1 -1.2 -1.2 0.0 -1.2 -0.5 -1.5 -0.6 Skin oil content -2.3 -0.4 -0.7 1.1 -1.1 0.3 -1.2 0.2 Skin flexion -2.0 -1.2 -1.2 0.0 -1.3 -0.4 -1.6 -0.8

TABLE 7

Period (Type B) 1 week 2 weeks 3 weeks 4 Weeks Measurement area ball nose ball nose ball nose ball nose Skin moisture -1.5 -1.0 -1.9 -1.7 -2.9 -2.4 -3.0 -2.3 Skin oil content -1.1 -0.4 -1.9 -1.2 -3.3 -2.4 -3.5 -2.2 Skin flexion -1.7 -1.0 -1.9 -1.6 -3.0 -2.4 -3.1 -2.4

 As can be seen in Table 6, when the soap according to the present invention is used, the skin moisture content, skin oil content and skin curvature are improved, and the condition of dry or rough skin is improved as the use time passes. . In particular, it was found to be good for dry skin or sensitive skin.

In addition, as shown in Table 7, in the case of using a commercially available natural soap, the skin moisture (moisturizing) and the skin oil content is reduced, the condition of weak dry or normal skin as the time of use is weak or dry It appeared to deteriorate, and the skin curvature was also changed from normal to rough.

⑤ skin itching symptoms

Skin itching symptoms were assessed by dividing the red spots or itching according to personal preference after using soap, divided into three stages (yes, normal, none).

The obtained results are shown in Table 8 below.

[Table 8]

 Itching Stinging have is average none A type One 29 Type B 4 4 19

 As can be seen in Table 8, type B soap showed symptoms of itching or sting depending on the subject, but it was found that the symptoms of itching or stinging were good when using type A soap. there was.

⑥ aroma

⑦ Skin Foam

⑧ Soap Cleaning

Aroma fragrance, skin foam and soap cleansing ability were evaluated according to personal preference after using soap and divided into 5 stages (very good, good, normal, bad, very bad).

The obtained results are shown in Table 9 below.

TABLE 9

As can be seen in Table 9, it can be seen that the A-type soap according to the present invention has a better cleaning power and foaming power than the natural soap type B commercially available in the market.

Figure 1 schematically shows a soap manufacturing method according to the present invention.

Figure 2 schematically shows a method for verifying the efficacy of the manufacturing process to obtain an extract of seaweed.

3 is a graph showing the yield (%) of the crude extract extracted using the deep sea water and the general water.

Figure 4 is a graph showing the polyphenol content of the crude extract extracted using deep sea water and normal water.

FIG. 5 is a graph showing the electron scavenging ability (%) of the DPPH free radicals of crude extracts extracted using deep sea water and normal water.

Figure 6 is a graph showing the antimicrobial effect of Bacillus subtilis of crude extracts using deep sea water and normal water.

Figure 7 is a graph showing the antimicrobial effect of Staphylococcus aureus of crude extracts using deep sea water and normal water.

8 is a graph showing the antimicrobial effect of Escherichia coli of crude extracts using deep sea water and normal water.

9 is a graph showing the whitening effect of the crude extract using the deep sea water and normal water.

Claims (14)

Base oil; Caustic soda-ocean deep water solution in which caustic soda is dissolved in desalted seawater with a hardness of 100 to 200 ppm; And Marine collagen obtained from fish scales, sponges or mixtures thereof, gelation temperature of 15 to 25 ℃, weight average molecular weight of 500 to 1500 Soap composition comprising a. The method of claim 1, The caustic soda and deep sea water mixing ratio is 1: 1.5 to 3 (caustic soda weight: deep sea water weight) by weight. The method of claim 1, The base oil is at least one selected from the group consisting of coconut oil, palm oil, castor oil, olive oil, sweet almond oil, grape seed oil, jojoba oil, sunflower seed oil, camellia oil, avocado oil, calendura oil, and rice bran oil. Composition. The method of claim 3, The base oil is 27 to 33 parts by weight of coconut oil, 27 to 33 parts by weight of palm oil, 13 to 17 parts by weight of castor oil, 9 to 13 parts by weight of olive oil, 9 to 13 parts by weight of sweet almond oil, And 3-7 parts by weight of grapeseed oil. The method of claim 1, The mixing ratio of the base oil and the caustic soda-ocean deep water solution is 1: 0.1 to 0.8 (base oil weight: caustic soda-ocean deep water solution weight) by weight. delete The method of claim 1, The content of the marine collagen is a soap composition of 0.01 to 1% by weight, based on the total weight of the soap composition including the base oil, caustic soda-ocean deep water solution and marine collagen. The method of claim 1, Neroli, Lemon, Sweet orange, Lavender, Cypress, Jasmine, Tea tree, Pine, Ylangylang At least one essence oil selected from the group consisting of Grapefruit, Rose and Rosemary; And At least one preservative selected from the group consisting of tocopherol, grapefruit seed extract, rose marioyl extract and citric acid Soap composition further comprising at least one selected from the group consisting of. The method according to any one of claims 1 to 5, 7 and 8, Seaweed extract obtained by extracting seaweed with water, desalted deep seawater, a low-cost alcohol having 1 to 5 carbon atoms, hexane, chloroform, and acetone from at least one solvent selected from the group consisting of 0.001 to 0.1 based on the weight of the final soap composition. The soap composition further comprises an amount by weight. 10. The method of claim 9, The solvent is a desalted deep seawater, or a desalted deep seawater and water, a low-carbon alcohol having 1 to 5 carbon atoms, hexane, chloroform, acetone is a mixture of one or more selected from the group consisting of. Soap prepared from the soap composition according to any one of claims 1 to 5, 7 and 8. Soap prepared from a soap composition according to claim 9. Dissolving caustic soda in a desalted deep sea water having a hardness of 100 to 200 ppm to prepare a caustic soda-sea deep water solution; Mixing and stirring the caustic soda-ocean deep water solution and base oil; And Adding marine collagen to the mixture Comprising a soap manufacturing method. The method of claim 13, Seaweed extract obtained by extracting seaweed with water, desalted deep seawater, a low-cost alcohol having 1 to 5 carbon atoms, hexane, chloroform, and acetone from at least one solvent selected from the group consisting of 0.001 to 0.1 based on the weight of the final soap composition. And further adding in an amount by weight.

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