KR102389589B1 - Antifungal composition comprising spirits extract of Lespedeza cuneata and process for preparing the same - Google Patents

Abstract

The present invention relates to an antifungal composition for feed containing a Lespedeza cuneata extract and a method for preparing the same, and more particularly to an antifungal composition for animal feed containing a Lespedeza cuneata extract and a method for preparing the same, wherein eco-friendly and economical properties are secured by minimizing side effects and irritation and enhancing animal absorption, feed preservability, and safety for workers while inhibiting the propagation of various germs. The antifungal composition for animal feed according to the present invention is a natural antifungal agent prepared using plant extracts in an appropriate mixing ratio, and is environmentally friendly and non-irritating. By inhibiting the propagation of various germs without reducing the intake of animals, the preservability and safety of the feed are high, thereby providing a pleasant working environment for workers and their surroundings. Also, it is possible to provide an economic advantage of reducing mortality due to overacidosis.

Description

Antifungal composition for feed, comprising extract of sagebrush, and method for preparing the same

The present invention relates to an antifungal composition for feed comprising an extract of Yagwanmun, and a method for preparing the same, and more particularly, to minimize the induction of side effects and irritation, inhibit the propagation of various bacteria, absorb animals, preserve feed, and provide workers with It relates to an antifungal composition for animals, including an eco-friendly and economical extract of wild chrysanthemum, and a method for manufacturing the same by increasing safety.

As a drug to prevent decay of substances, antiseptic is to prevent decomposition of animal and plant organic matter by the action of microorganisms, and antifungal is a drug added for preservation for the purpose of preservation. The microorganisms that cause spoilage include fungi, yeasts, and lower microorganisms, bacteria.

Antifungal properties are essential to maintain the quality of various product groups such as food, cosmetics, pharmaceuticals, and daily products for a long period of time, and should not harm the human body, and should not damage the quality of products due to the addition of antifungal properties.

Conventionally, chemically synthesized antifungal agents have been generally used in food and pharmaceuticals. For example, chemical antifungal properties such as calcium propionate, sodium benzoate, sodium nitrite and sorbic acid are used as food antifungal properties, and methyl paraben and ethyl paraben are used as cosmetic antifungal properties. , Alkyl parabens such as propyl paraben and butyl paraben, chemical synthetic antifungal agents such as quaternium-15, imidazolidinyl urea, and phenoxyethanol became

Since these chemical synthetic antifungal properties are very harmful to the human body or livestock when the amount is exceeded, their use is regulated by the Pharmaceutical Affairs Act or the Food Sanitation Act. Therefore, interest is focused on the development of antifungal properties that can strongly prevent the deterioration and spoilage of products caused by microorganisms without causing toxicity or side effects to the human body. .

However, formulations made from natural substances are known to have low toxicity and irritation to the human body, high biodegradability, and are known to be environmentally friendly, whereas natural preservatives developed so far have problems such as low economic feasibility or limitations in the range of use due to a narrow antibacterial spectrum or physical properties. has a

Accordingly, it is necessary to develop natural antifungal properties that are harmless to the human body and are rich in functionality.

Korea Publication No. 10-2019-0090715 (August 02, 2019) Korea Publication No. 10-2020-0050121 (May 11, 2020) Korea Registration No. 10-0983791 (September 16, 2010) Korean Patent Registration No. 10-1710589 (February 21, 2017)

The inventors of the present inventors have studied intensively using natural materials as a material to develop an eco-friendly and economical antifungal composition for animals that is less irritating and has less irritation and improved problems according to the prior art. As a result, the feed composition described below can satisfy the above requirements. found and led to the completion of the present invention.

Accordingly, an object of the present invention is to provide an antifungal composition for animals that is environmentally friendly and economical with less irritation and improved problems according to the prior art.

The above object of the present invention is

60 to 80 parts by weight of Yagwanmun extract, 10 to 25 parts by weight of Hwangchil extract, 3 to 7 parts by weight of cypress extract, 3 to 7 parts by weight of licorice extract, 0.1 to 1.5 parts by weight of vitamin E acetate, and 0.1 to 0.3 parts by weight of sea salt It can be achieved by an antifungal composition for animal feed, characterized in that it and a method for producing the same.

The antifungal composition for animal feed according to the present invention is manufactured using a plant extract of an appropriate mixing ratio and is eco-friendly because it is non-irritating, does not reduce the intake of animals and inhibits the propagation of various bacteria, thereby increasing the preservation and safety of the feed. And it is possible to provide an economical advantage of providing a comfortable working environment around life, and reducing mortality due to hyperacidosis.

1 is a manufacturing process diagram for an antifungal composition for animal feed according to the present invention.

The present invention, in one aspect,

60 to 80 parts by weight of Yagwanmun extract, 10 to 25 parts by weight of Hwangchil extract, 3 to 7 parts by weight of cypress extract, 3 to 7 parts by weight of licorice extract, 0.1 to 1.5 parts by weight of vitamin E acetate, and 0.1 to 0.3 parts by weight of sea salt It provides an antifungal composition for animal feed, and a method for preparing the same.

Hereinafter, the antifungal composition for animal feed according to the present invention and a manufacturing method thereof will be described in more detail with reference to the accompanying drawings.

The terms or words used in the present specification and claims are not to be construed as limited in their ordinary or dictionary meanings, and on the principle that the inventor can appropriately define the concept of the term in order to best describe his invention. It should be interpreted as meaning and concept consistent with the technical idea of the present invention. Therefore, since the embodiments described herein and the configurations shown in the drawings are only the most preferred embodiment of the present invention, it is understood that there may be various equivalents and modifications that can be substituted for them at the time of the present application. shall.

In the present invention, the feed composition for animals is expressed in parts by weight or weight%, and the sum of individual components may include an impossible part exceeding 100% by weight according to the calculation formula, but such excess or impossible part is the present invention. It is obvious that it is not included in the scope of the invention, and in particular, corrections to clarify this in the claims, for example, corrections that add a proviso that a range exceeding 100% by weight is excluded from the scope of the present invention is an ambiguous description It is only intended to explain, and it is obvious that the nature or scope of the present invention is not added or subtracted.

Conventional feed additives are manufactured by containing chemicals such as propionic acid, which is a strong acid, as a preservative, so that not only the feed intake rate of livestock falls, but also the problem of animal death due to peracidosis is often caused, and also livestock handling it The odor and irritation on the body of workers not only caused side effects that could cause blindness in severe cases, but also caused complaints from neighbors due to the strong irritating odor. Provides an eco-friendly and economical animal feed composition by providing an antifungal composition for animals, which minimizes the above side effects and irritation, inhibits the propagation of various bacteria, and improves animal absorbency, feed integrity, and safety for workers. There are technical features to doing this, which can provide various advantages in an environmentally friendly way.

As shown in FIG. 1 , the method for producing the antifungal composition for animal feed according to the present invention largely includes a pretreatment step of plant material, a mixing step, an extraction step, an additive mixing step, a formulation step and a packaging step. The individual steps are described in detail below.

a) pretreatment step of plant material

First, plant materials obtained through various routes are washed with a high-pressure sprayer using purified water, etc. to facilitate processing in the subsequent step to filter out dust, etc., and then pre-treated by filtering foreign substances. The plant materials include Yagwanmun, Hwangchil tree, and Cypress.

b) compounding step

As a step of blending the plant material in a certain ratio, the mixing ratio of the plant material is 60 to 80 parts by weight of Yagwanmun, 10 to 25 parts by weight of hwangchil, 3 to 7 parts by weight of cypress, and 3 to 7 parts by weight of licorice based on 100 parts by weight of the total formulation. It is preferable that it is a weight part.

Among them, Yagwanmun ( Lespedeza cuneata ) is mainly distributed in Korea, Japan, Taiwan, India, and Australia, and is also called Bisuri, Nougeun, Hojija, and Sanchaeja. Yagwanmun has a bitter and slightly bitter taste, has a flat nature, is non-toxic, and acts mainly on the lungs, liver, and kidneys. It is known to be excellent in improving the function of kidney, liver and intestines, and improving blood circulation. It removes blood clots and relieves swelling. It has been reported to improve blood circulation. In addition, it has been reported that it is effective in reducing fever, killing insects in the stomach, treating boils on the breast, snake bite wounds, and red and bloodshot eyes, and is effective in gastric ulcer and prolapse. There are pinitol, flavonoid, tannin, and Bsitosterol in the leaves, roots, and stems of Yagwanmun. These ingredients eliminate inflammation and clear phlegm, and Staphylococcus aureus, pneumococcus, and streptococcus. , has the effect of killing or inhibiting catarrhal bacteria. Yagwanmun can use leaves, stems, roots, etc.

In addition, the Hwangchil tree ( Dendropanax morbifera Lev. ) is an evergreen broad-leaved arboreous tree of the genus Araliaceae, and is native to Korea, growing up to 15 m or more in height. The leaves are alternate phyllotaxis, and depending on the amount of sunlight, the shape is not uniform in five different shapes. Young twigs are green and glossy, flowers bloom in June, and 7-19mm long fruits ripen in black. It is a temperate plant that grows in areas where the minimum temperature is -2℃ or higher and the annual average temperature is 12~15℃ or higher. Hwangchil tree worldwide is a rare species that grows only in the inland and coast of the southwestern part of the Korean Peninsula, islands, and Jeju Island, and its number is extremely limited at present. Currently, there are island areas such as Shinan, Jindo, Wando, and Jeju Island, and coastal areas include Haenam, Jangheung, Gangjin, Goheung, Seungju, Gwangyang, and Yeosu. In addition, the clove component contained in Hwangchil has a variety of pharmacological effects as well as its value as a sedative that makes the mind and body clear and comfortable. Hwangchil can use leaves, stems, branches, etc.

Cypress ( Chamaecyparis obtusa ) is an evergreen arboreous tree of the Arboraceae family, also called Hinoki, Hinoki, and Hinoki. The height is 30-40 m, the width is about 1-2 m, the bark is reddish-brown, and small needle-shaped leaves are dense on the branches. It is distributed in Japan and southern Korea, and contains γ-cadinene, α-terpineol and borneol, and leaves and wood contain 1% essential oil. . Cypress produces antibodies against antigens to improve the body's immunity, and is widely used as a natural insecticide to combat house dust mites that cause allergic diseases such as atopy, asthma, and rhinitis. It is better to use cypress extracts from leaves, stems, or branches. The cypress extract may be used alone or in the form of a mixture. Cypress leaves, stems, branches, etc. can be used.

Licorice ( Glycyrrhiza uralensis FISCH.) is a perennial herb belonging to Leguminosae , Glycyrrhiza uralensis Fisch. and G.inflata Bat. and dried roots, roots and leaves of G. glabra L. It is known to have anti-cancer, anti-diabetic, antioxidant, anti-inflammatory, anti-ulcer, and detoxifying effects.

Since the animal feed composition according to the present invention contains an extract of a natural material rather than a chemical synthetic agent, as can be seen from the results of Examples and Test Examples to be described later, there is no irritation or toxicity to the skin, so it has excellent stability as well as Since it has functionalities such as antioxidant properties and antibacterial effects, it can be efficiently used as a feed additive requiring such functionality.

The desired effect in the present invention is to maximize the functionality including the antioxidant effect and antibacterial effect by mixing in an optimal ratio in consideration of the effective dose and side effects of each of the above natural ingredients, and In this case, there is a fear that the effect may decrease.

The antioxidative and antibacterial function of Yagwanmun is weak at 60 parts by weight or less based on 100 parts by weight of the total formulation, and it is difficult to achieve proper harmony with other ingredients at 80 parts by weight or more. If the amount of the hwangchil is less than 10 parts by weight, the anti-inflammatory properties and neutralizing function are weak, and in the amount of 25 parts by weight or more, there is no synergistic effect according to the amount added, so economical efficiency is a problem. Cypress has a weak clove function when it is 3 parts by weight or less, and economical efficiency becomes a problem when it is 7 parts by weight or more. Licorice can be included in 3 to 7 parts by weight to optimally exhibit anti-inflammatory effects, neutralizing effects, stable harmony of the overall formulation, and various functionalities.

c) extraction of plant material

Then, the mixed plant material is extracted with alcohol to obtain an extract.

Alternatively, each plant material may be individually extracted and then used by appropriately mixing within the range set above. In the present invention, in order to simplify the procedure, for convenience, a method of extracting the plants by mixing will be mainly described.

The mixed extract of Yagwanmun, hwangchil tree, cypress and licorice is preferably obtained by leaching at room temperature for 2 to 4 months using alcohol, and the alcohol has an alcohol concentration of 35 to 95% (v / v) ethanol. It is more preferable that it is a diluted alcohol.

Alternatively, the plant material may be extracted by hot water extraction, cold extraction or hot extraction with the addition of an extraction solvent. In this case, the extract can be obtained by adding and mixing 2 to 20 times the extraction solvent in a weight ratio with respect to the plant material, followed by extraction at 70 to 125° C. for 2 to 24 hours, filtration and concentration.

Here, at least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, alcohol, or a mixture thereof may be used as the extraction solvent. Methanol, ethanol, etc. can be used as a C1-C4 lower alcohol, and butylene glycol, propylene glycol, pentylene glycol, etc. can be used as a polyhydric alcohol. And as the mixture, a mixture of water and a lower alcohol, a mixture of water and a polyhydric alcohol, a mixture of a lower alcohol and a polyhydric alcohol, or a mixture of water and a lower alcohol and a polyhydric alcohol may be used.

Filtration can be performed, for example, by filtering out solid foreign substances and particles using a filtration membrane, paper filter paper, hemp, non-woven fabric or cotton, or by ultrafiltration, freezing filtration, centrifugal separation, and the like. In addition, filtration may be performed by first filtering using gauze and then performing secondary filtering using Whatman paper.

The filtrate can then be used in a liquid phase in a subsequent step, and it is preferable to obtain an extract by subsequent evaporation to prepare a solid product.

d) additive mixing step

Additives, for example, 0.1 to 1.5 parts by weight of vitamin E acetate and 0.1 to 0.3 parts by weight of sea salt, are added to the plant extract and mixed.

In addition, a small amount of a separate additive may be added to prevent deterioration of quality or to increase functionality. Examples of such additives include preservatives, anticoagulants, binders, emulsifiers, antioxidants, antifungal agents, flavoring agents, and the like. .

Examples of the antifungal agent include glyceryl caprylate (GMCY), 4-hydroxyacetophenone, hexanediol (1,2-hexandiol), and ethylhexyl glycerin.

e) formulation step

Then, the mixture mixed with the additive can be formulated in a certain shape, for example, the mixture is diluted with purified water and the like to be formulated into a liquid product, or the mixture is molded with a ventilation machine to have an average diameter of 1 to 20 mm. It can be formulated by aging and drying at room temperature for 7 to 20 days so that the moisture content of the is 3 to 15%.

Then, the formulated antimicrobial composition is sterilized by a conventional method to improve the preservation of the product, and is usually sterilized at 90 to 125° C. for 5 to 60 minutes to kill microorganisms.

f) packaging step

Finally, the product of the antimicrobial composition for animals that has undergone a sterilization process is packaged in a predetermined unit and stored in storage until shipment.

The antifungal composition for animals may be preferably fed by mixing 1 to 10% by weight based on the weight of the formulated feed.

Formulated feed can be used without any particular limitation, and includes commercially available general feed, loess, green barley silage, brewer's meal, leeks, wheat bran, rice bran, cottonseed meal, malt, molasses, yeast culture, minerals and vitamins, minerals, and salt. may be included.

In addition, it is more preferable that the formulated feed contains 1 to 10% by weight of antifungal properties for animals, 10 to 40% by weight of rice bran, 10 to 40% by weight of wheat bran, 10 to 40% by weight of grains, and 1 to 3% by weight of salt. can do.

The antifungal composition for animals prepared in this way has no strong odor, has excellent antibacterial action, and contains abundant calories and nutrients, so it can promote the safe growth of livestock, and can be used in combination with compound feed, It can be usefully used not only for raising livestock such as pigs, sheep, goats, ducks, geese, and chickens, but also for fish farming. In addition, it can be used as feed for companion animals such as pet dogs and cats or edible insects.

<Example>

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are provided to help the understanding of the present invention and do not limit the content and scope of the present invention.

Statistical analysis: All analysis values obtained in the test were presented as average values for each treatment group. Statistical analysis was performed using the General Linear Model (GLM) of SAS (SAS Instityte, 1996). Treatment is the main effect, and the cow is the experimental unit for the analysis of all parameters. The efficacy of other steps was also evaluated. The significance test was analyzed using Duncan's multiple range test for statistical analysis.

Examples 1-6: Preparation of antibacterial composition for animals

After washing and pre-treating Yagwanmun, Hwangchil, Cypress and Licorice, mixing as shown in Table 1 below, and then leaching at room temperature for 3 months using 50% diluted alcohol to obtain an extract, and then mixing with additives, After sterilization, it was divided into 10 kg each to prepare liquid antifungal properties for animals.

ingredient Content (weight ratio) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 night gate 60 65 70 70 75 80 35 Hwangchil 25 25 20 20 15 10 30 Cypress 7 6 4.5 6.5 3 3 33 licorice 7 3.8 4.5 3 5.2 6.5 - vitamin E acetate 0.9 0.1 0.8 0.3 1.5 0.3 - sea salt 0.1 0.1 0.2 0.2 0.3 0.2 - propionic acid - - - - - - 2 Sum 100 100 100 100 100 100 100

Examples 7-12: Preparation of compound feed

The following compounded feed was prepared by selecting the antifungal agent for animal feed of Example 3 as a representative test group.

ingredient Content (Kg) Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Comparative Example 2 rice bran 18 14 10 33 40 38 50 pulse river 40 40 40 35 10 40 30 grain 40 40 37 20 37 10 19 antifungal One 5 10 10 10 9 - salt One One 3 2 3 3 One Sum 100 100 100 100 100 100 100

Test Example 1: Measurement of DPPH antioxidant effect

DPPH free radical scavenging experiments were performed on the samples of Examples 1 to 6 and Comparative Example 1. DPPH (1,1-diphenyl-2-picrylhydrazyl) itself is a very stable free radical, measuring a dark purple compound that exhibits specific light absorption at 517 nm, and is an antioxidant with radical scavenging activity. Antioxidant activity can be easily measured due to quantitative decolorization by Since the scavenging activity by these radicals is correlated with antioxidant activity including lipid peroxidation inhibitory activity, it is widely used in the search for antioxidants. Measurement of the antioxidant activity (electron donating abilities, EDA) of the sample was measured by modifying the method of Blois. 0.5 ml of 0.2 mM DPPH (1,1-diphenyl-2-picrylhydrazyl) was added to 20 ml of each sample solution, stirred, and left for 30 minutes, and then absorbance was measured at 517 nm. The electron donating effect was expressed as a decrease in absorbance between the addition and non-addition groups of the sample solution. The resulting free radical scavenging ability was calculated by the following formula.

Free radical scavenging activity (%) = {1-(B/A)}ㅧ100

In the formula, A: the absorbance of the control well not treated with the sample, and B: the absorbance of the well of the experimental group treated with the sample. The results are shown in Table 3.

Ex1 Ex2 Ex3 Ex4 Ex5 Ex6 Comp. Ex1 DPPH free radical-scavening activity (%)
87.31
85.50
88.16
86.21
84.66
82.38
52.32

Through the above results, it was confirmed that the samples of Examples 1 to 6 exhibited superior DPPH free radical scavenging ability compared to those of Comparative Examples.

Test Example 2: Antibacterial activity test

The antibacterial and sterilizing powers of the compositions according to Examples 1 to 6 were tested by conducting a disc paper test.

The test strain was dispensed into a petri dish and spread over the entire medium using a glass rod, and then a paper disc with a diameter of 5 mm was placed on it. And 30 μl of the composition of each example was put on a paper disc. After overnight incubation at 30°C, the inhibition zone was checked to determine the antibacterial properties. The results are shown in Table 4 below.

test strain Inhibition zone(d) : cm Ex1 Ex2 Ex3 Ex4 Ex5 Ex6 Comp. Ex1. Staphylococcus aureus
ATCC 10537 2.7 2.5 3.0 2.8 2.6 2.6 1.2 Staphylococcus aureus
ATCC 256E 2.6 2.7 2.9 2.8 2.7 2.6 1.4 Salmonella typhimurium
KCTC 1925 2.7 2.8 3.2 3.0 2.8 2.7 1.7 Candida albicans
ATCC 10231 2.7 2.9 2.9 3.2 2.8 2.6 2.1

As can be seen from the above test results, the feed composition according to the present invention has a different degree, but has excellent antibacterial action against the test bacteria, so it can be used as an additive for feed that requires it.

Test Example 3: Measurement of growth inhibitory activity of mold

Fungi, Trichophyton rubrum ( Trichophyton rubrum , KCTC 6345 ), Mycosporum audouinii , KCTC 6346 ), Trichophyton ferrugineum ( Trichophyton ferrugineum , KCTC6351 ), Epidermophyton floccosum , Epidermophyton floccosum KCTC 6586) and Trichophyton mentagrophytes (KCTC 6077) were tested for growth inhibitory activity in 5 species. The fungal growth inhibitory activity was assayed by the Paper disc diffusion method. After inoculating each strain on Sabouraud's agar medium, each composition (Examples 1-6, treatment concentration 100 ㎍) was directly treated on a paper disc. After placing the filter paper on the medium inoculated with each bacteria, aerobic culture at 28 ° C. for 5 days, looking at the size of the clear zone formed around the filter paper, the antibacterial activity was checked, and the results are shown in Table 5 it was

test strain Inhibition zone(d) : cm Ex1 Ex2 Ex3 Ex4 Ex5 Ex6 Comp. Ex1. Trichophyton rubrum 3.0 3.0 3.3 3.2 3.2 3.3 0.7 Microsporum audouinii 3.1 3.2 3.4 3.2 2.6 2.9 0.8 Trichophyton ferrugineum 2.8 3.0 3.5 3.3 2.7 3.0 0.9 Epidermophyton floccosum 2.8 3.3 3.8 3.1 2.8 2.8 1.1 Trichophyton mentagrophytes 3.1 3.2 3.6 3.4 3.0 2.7 0.9

The size of the growth inhibitory ring by the composition of Example was 2.6 cm or more, and it was found to have excellent fungal growth inhibitory activity. This antibacterial activity showed a similar pattern in the rest of the fungi.

Test Example 5: Acute toxicity test

An acute toxicity test was performed on the compositions of Examples 1 to 6, and the presence or absence of toxicity was observed. For laboratory animals, 60 male and 95 female SD rats of 4 and 5 weeks of age were used and tested using the composition of each Example and distilled water as a negative control. First, the rats were subjected to a temperature of 22° C., a relative humidity of 53%, a light-dark cycle of fluorescent lighting (09:00 on-18:00 off), and a period of about one week in a laboratory breeding box equipped with an illuminance condition of 150-300 Lux. After acclimatization over a period of time, only healthy animals were selected and divided into groups so that the average body weight was matched, and administered orally at a dose of 20ml/kg once a day for 14 days. , autonomic nerves, weight changes, and the presence or absence of dead animals were checked.

According to the experimental results, there was a change within 5% of body weight during the experiment period, but there was no significance. Since it was impossible to calculate the lethal dose, the LD ₅₀ was found to be 20ml/kg BW or more, and no specific general symptoms or specific lesions were observed at autopsy.

Test Example 6: Pig breeding according to feed feeding

As shown in Table 6 for high quality development such as productivity and meat quality characteristics of pigs by feeding feeds according to Examples 7 to 12 and Comparative Example 2, 70 sows with an average weight of 60 ㅁ 3 kg were tested in group 7 They were isolated in spheres and bred for 60 days, 10 each. The test feed was analyzed by the AOAC (1996) method, and feed and water were allowed to be freely consumed during the feeding test.

For the test group, the body weight and feed intake rate of pigs fed individual feeds were investigated. The starting body weight was measured on the test start day, and the ending body weight was measured on the test end date. The weight gain is the value obtained by subtracting the starting weight from the end weight. The amount of feed intake was measured every morning before feeding, and the amount of feed fed the previous day was measured. The results are shown in Table 6 below.

Test Items Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Comparative Example 2 Starting weight (Kg) 60.76±0.54 61.21±0.45 60.46±1.12 60.34±1.11 60.12±1.52 60.66±1.23 60.14±1.25 End weight (Kg) 110.52±1.26 112.52±1.14 113.23±1.38 109.45±1.27 110.27±1.25 107.33±1.24 92.37±1.25 Weight gain (Kg) 49.76±0.55 51.31±0.45 52.77±0.12 49.11±0.07 50.15±0.21 46.67±0.21 32.23±0.12 Weight gain per day (g) 829.33±1.32 855.16±2.28 879.50±2.11 818.50±2.55 835.83±2.58 777.83±2.51 537.16±2.65 Feed intake (g) 2,131.2
±3.15 2,156.3
±3.36 2,185.5
±4.65 2,085.2
±3,32 2,142.3
±2.85 2,034.5
±4.35 1,876.5
±2.71 Feed demand rate 2.56±0.25 2.52±0.38 2.44±0.51 2.54±0.58 2.56±0.91 2.61±0.18 3.49±0.33

As a result of the experiment, as shown in Table 6, the end weight, weight gain, and daily gain were significantly higher (P<005) in the feed group of Example than in the control group. There was no significant difference in feed intake. When looking at these results, it was confirmed that the feeding of the feed according to Examples 7 to 12 increased the amount of gain due to the increase of the feed intake.

As such, although the present invention has been described with reference to the drawings and the specification for preferred embodiments of the present invention, it is apparent that various modifications and equivalent implementations are possible by those skilled in the art.

Claims (4)

Contains 60 to 80 parts by weight of Yagwanmun extract, 10 to 25 parts by weight of Hwangchil extract, 3 to 7 parts by weight of cypress extract, 3 to 7 parts by weight of licorice extract, 0.1 to 1.5 parts by weight of vitamin E acetate, and 0.1 to 0.3 parts by weight of sea salt, Each of the extracts is obtained by leaching at room temperature for 2 to 4 months using alcohol, and the alcohol is an antifungal composition for animal feed, characterized in that 35%-95% alcohol. delete a) pre-treating to filter out foreign substances after washing the plant material;
b) blending the plant material in a certain proportion;
c) extracting the mixed plant material with alcohol to obtain an extract;
d) mixing the extract with an additive to obtain a mixture;
e) formulating the mixture into a predetermined shape; and
f) packaging the formulated product in a predetermined unit;
The plant material is a method of producing an antifungal composition for animal feed, characterized in that it comprises 60 to 80 parts by weight of Yagwanmun, 15 to 25 parts by weight of hwangchil, 3 to 6 parts by weight of cypress, and 3 to 6 parts by weight of licorice. 4. The method according to claim 3,
The extraction step is performed by leaching the mixed plant material at room temperature for 2 to 4 months using alcohol, and the alcohol is 35%-95% alcohol. Method for producing an antifungal composition for animal feed.

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