KR20190090715A - An animal feed composition comprising phlorotannin and product for animal - Google Patents

Abstract

The present invention relates to a composition for animal feed having an antioxidant and anti-inflammatory activity, containing phlorotannin extracted from brown algae. More specifically, the present invention relates to the composition and a product which can be safely applied routinely using natural-derived raw materials, and thus have a function of improving hair luster, hair smell, defecate odor, defecate discoloration, and overweight.

Description

Animal feed composition comprising phlorotannin and product for animal

The present invention relates to an animal feed composition and an animal product containing phlorotannin as an active ingredient having antioxidant and inflammation improving effects. The composition and the animal product of the present invention have the function of giving shine to animal hair through antioxidative effect and inflammation improving effect, improving animal odor, color change, obesity, and contributing to psychological stability. The present invention also relates to an animal feed composition and an animal product which can be safely and safely taken for a long period using a natural ingredient as a main ingredient.

As one family increases and average income increases, more families are growing. As the number of companion animals increases, the size of the related industry is also increasing. According to the status of companion animal feed industry in Korea Rural Economic Institute, the feed industry, which was 250 billion won in 2012, increased to 460 billion won in 2016. In addition, the average lifespan of companion animals is also increasing due to the increase in vaccination and the improvement of food quality through the improvement of the awareness of the family raising the companion animal.

However, companion animals are consuming excessive amounts of food and snacks and consuming human food, resulting in overweight or nutritional imbalance. An increase in the weight of the animal stimulates the joints, and sustained stimulation can also lead to inflammatory diseases and degenerative arthritis. The incidence of arthritis and inflammatory diseases can cause psychological anxiety during the lifetime of companion animals and can have a negative impact on companion animal emotions. As a result, interest in degenerative diseases due to aging such as arthritis and dementia of companion animals is increasing among the pet animals, and interest in daily health care is also increasing.

Inflammation is caused by pathogens, stimulants, etc. It inhibits early cell damage and regenerates tissue. However, when inflammation becomes worse, it also causes diseases such as inflammatory diseases. Immune strengthening is needed to prevent inflammation from developing into disease, which means that normal nutrient intake and functional foods are needed. In addition, since animals can not express their intentions unlike humans, it is necessary to routinely treat foods that are usually taken, antioxidants in snacks, and functional foods with anti-inflammatory functions.

Korean prior art 10-1777842 discloses an animal feed, and Korean Patent Laid-Open No. 10-2005-0099436 discloses an animal feed containing sea tangle added thereto, which comprises an antiallergic and anti- Containing animal feed additive.

The present invention provides a composition for animal feed containing fluorotannin having antioxidant and anti-inflammatory functions derived from brown algae, unlike the existing patent, and provides an animal product including the functional ingredient.

(Public patent 1) Republic of Korea Patent No. 10-1777842 (Open patent 2) Republic of Korea Patent Publication No. 10-2005-0099436

The present invention provides a composition for animal feed comprising an antioxidant, an anti-inflammatory function, and a product for animals, thereby improving the daily health care of animals and reducing the incidence of inflammatory diseases, We want to improve the satisfaction of the liver. We also provide various kinds of products such as powdered products, animal feed, and snacks so that users can conveniently access and apply them.

In order to achieve the above object, the present invention provides a composition for animal feed comprising phlorotannin as an active ingredient.

The present invention also provides an animal product comprising said composition.

The present invention also provides a method of using a composition comprising phlorotannin as an active ingredient as an animal feed.

The composition for animal feed containing the phlorotannin of the present invention as an active ingredient contains a naturally occurring material and does not contain a preservative or a chemical substance so that it can be used safely and has no concern about side effects and is applied in various forms such as feed and snack This is possible. In addition, the product of the present invention is applied to a product that is routinely used to help an animal in daily health care and has an effect of improving immunity.

1 is a view showing Product Example 1 of Example 2. FIG.
2 is a view showing a packaging state of the product example 2 of the third embodiment.
3 is a view showing Product Example 2 of Example 3. FIG.
Figure 4 is a diagram showing the antioxidant effect of the composition of Example 1.
5 is a view showing the NO production inhibitory effect of the composition of Example 1.
6 is a diagram showing a PGE 2 production inhibitory effect of the composition of Example 1.
7 is a view showing a COX-2 production inhibitory effect of the composition of Example 1.

Hereinafter, the present invention will be described in detail.

The number of households with one person is increasing, the number of households raising their companion animals is increasing, and due to the development of feed and medicine for companion animals, the survival age is getting longer and the problem of health care of aged companion animals is emerging. Degenerative and inflammatory diseases such as arthritis, stomatitis, and dementia that occur in aged companion animals can cause life-style inconvenience and adversely affect people's emotions. Obesity and nutritional imbalance caused by irregular nutrition of companion animals may also exacerbate the health of animals.

Accordingly, the present invention provides a composition for animal feed having anti-inflammation and antioxidant activity, and has developed a product utilizing the composition, thereby completing the present invention.

The present invention provides a composition for animal feed comprising a phlorotannin compound as an active ingredient.

The composition for animal feed containing the phlorotannin of the present invention as an active ingredient has an antioxidant and anti-inflammatory function and improves the health of animals.

The present invention provides a method of using a composition comprising phlorotannin as an active ingredient as an animal feed. When the composition is used as a feed, the composition improves the health of the animal by having antioxidant anti-inflammatory function.

The animal feed composition may contain 20 to 100% by weight of a float tannin compound.

The composition for animal feed can be obtained by any conventional method as a naturally derived component. That is, they may be synthesized by using commercially available reagents or may be obtained by extracting and separating from natural products, particularly seaweeds.

The animal feed composition may be a seaweed extract. The composition for animal feed can be a spirit extract of seaweed.

The composition for animal feed comprising the above phlorotannin as an active ingredient can be extracted from seaweeds, especially brown algae, and specifically includes Eisenia bicyclis, Eisenia arborea, Eisenia desmarestioides (Eisenia desmarestioides) Eisenia galapagensis, Eisenia masonii, Ecklonia kurome, Ecklonia cava, Ecklonia stolonifera, Ecklonia maxima, Ecklonia maxima, Ecklonia radiata, Ecklonia bicyclis, Ecklonia biruncinate, Ecklonia buccinalis, Ecklonia caepaestipes, Ecklonia spp., Ecklonia spp. Ecklonia exasperta, Ecklonia fastigiata, Ecklonia brevipes, Ecklonia brevipes, arborea, Ecklonia latifolia, Eclonia muratii, Eclonia radicosa, Eclonia richardiana, and Eclonia wrightii It is extracted from one or more seaweeds selected from.

In addition to phlorotannin, the composition of the present invention may further contain 0 to 80% by weight of a component composed of mannitol, alginic acid, fucoidan, laminaran, and fucosterol, fucosanthin and the like as lipid components.

The composition for animal feed of the present invention may contain at least one selected from among the compounds represented by the following general formulas (1) to (12).

[Formula 1]

(2)

(3)

[Chemical Formula 4]

[Chemical Formula 5]

[Chemical Formula 6]

[Formula 7]

[Formula 8]

[Chemical Formula 9]

[Formula 10]

[Formula 11]

[Chemical Formula 12]

The composition for animal feed of the present invention may contain at least one kind of phlorotannin selected from the group consisting of the above-mentioned formulas (2), (4), (5) and (8) When the proper amount of the fluorotannin is contained, the antioxidant and inflammation improving effect are excellent.

The composition for animal feed according to the present invention preferably comprises 1 to 60% by weight of phlorotannin of formula (2), 0.3 to 20% by weight of phlorotannin of formula (4), 0.1 to 10% by weight of phlorotannin of formula And 0.1 to 5% by weight of a fluorotannine represented by the general formula (8).

The composition for animal feed according to the present invention comprises 0.1 to 3% by weight of phlorotannin of the formula (1), 5 to 63% by weight of the phlorotannin of the formula (2), 1 to 3% by weight of the phlorotannin of the formula A fluorotannine represented by the following general formula (6): 0.5 to 8 wt%, a fluorotannine represented by the following general formula (7): 0.1 to 1 wt% 0.1 to 3% by weight of tannin, 0.1 to 3% by weight of tannin, 0.1 to 2% by weight of tannin, 0.1 to 2% by weight of a fluorotannine of the formula To 4% by weight.

The composition of the present invention comprises 20 to 100% by weight of phlorotannin, 0 to 30% by weight of mannitol, 0 to 25% by weight of alginic acid, 0 to 25% by weight of fucoidan, 0 to 10% by weight of fucosterol, 0 to 10% , And 0 to 10% by weight of laminaran.

The composition of the present invention comprises less than 20 to less than 100% by weight of phlorotannin, greater than 0 to 30% by weight of mannitol, greater than 0 to 25% by weight of alginic acid, more than 0 to 25% by weight of fucoidan, more than 0 to 10% From more than 0 to 10% by weight, and from 0 to 10% by weight of laminaran.

The composition for animal feed of the present invention may be in the form of powder or liquid for easy addition to products such as animal feed, animal snack, and the like.

The present invention provides an animal product comprising the composition for animal feed comprising the above-mentioned fluro tannin.

The animal product of the present invention can be used in combination with a composition for animal feed comprising phlorotannin and various auxiliary agents such as amino acids, inorganic salts, vitamins, antibiotics, antimicrobials, antioxidants, antifungals, enzymes, living microbial preparations and the like as auxiliary components; Cereals such as ground or crushed wheat, oats, barley, corn and rice; Vegetable proteins such as rapeseed, beans and sunflower; Animal proteins such as blood, meat, bone meal and fish meal; And various sugar and dairy products such as various powdered milk and whey powders.

The animal product may contain 0.1 to 50% by weight, more preferably 0.2 to 10% by weight, of the composition for animal feed containing phlorotannin, based on the total weight of the product, but the blending amount is not limited to the above range. When the animal product of the present invention contains the animal feed composition in the above amount, it can provide the antioxidant, anti-inflammatory effect of the animal, improve the health care, and improve the immunity.

The present invention relates to a composition for animal feed comprising collagen, flaxseed extract, calcium, iron, vitamin B12 and vitamins such as vitamin E, copper, chicken, pig gelatin, rice flour, soy flour, wheat flour, minerals, , Glycerin, fish, and water. The present invention also provides an animal product comprising at least one member selected from the group consisting of glycerin, fish and water

The product preferably contains 1 to 50% by weight of the animal feed composition of the present invention, 40 to 80% by weight of collagen, 1 to 4% by weight of flaxseed extract, 5 to 15% by weight of calcium, 0.1 to 0.8% by weight of iron, To 0.01% by weight of vitamin E, 0.1 to 0.3% by weight of vitamin E, and 0.05 to 0.1% by weight of copper. In this case, the occurrence of inflammatory diseases of animals is alleviated and the animal's health is improved.

The animal product of the present invention can be commercialized as animal feed in powder or granular form, or snacks such as canned food and canned food.

The animal product of the present invention may contain 80-97% by weight of at least one member selected from the group consisting of chicken, pig gelatin, rice flour, soy flour and wheat flour. In this case, there is an advantage that it is easy to commercialize while providing balanced nutrition to animals.

The daily use amount of the phlorotannin contained in the animal product of the present invention is preferably about 0.1 to 100 mg, more preferably about 1 to 50 mg, but the dosage is not limited to the above range.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. However, the following examples and experimental examples are intended to further illustrate the present invention, and the scope of the present invention is not limited by the following examples and experimental examples. The following Examples and Experiments are appropriately modified and changed by those skilled in the art within the scope of the present invention.

Example 1. Preparation of a composition containing fluorotannine

In order to prepare an animal feed composition containing the phlorotannin of the present invention as an active ingredient, the brown algae were extracted from the brown algae by the following method.

Ecklonia cava was washed with distilled water to remove foreign matter, dried in shade, and then shattered. 500 g of the above menthol was added to the extraction vessel, 20 times of 30 wt% aqueous solution of menthol was added thereto, and the mixture was refluxed for extraction at 75 to 80 ° C for 4 hours to obtain an extract. The extract was filtered through a filter net of 10 mu m, and the filtered extract was concentrated under reduced pressure to obtain a primary extract concentrate. The primary extract concentrate was dried with a vacuum drier and pulverized to obtain 170 g of Composition 1.

The composition 1 was dissolved in hot water at 50 캜, adsorbed on an adsorption resin, washed with water, and eluted with a solvent to obtain a fluorotannine eluant. The eluate was filtered through a 1 탆 filter net, and the filtered extract was concentrated under reduced pressure to obtain a second extract concentrate. The second extraction concentrate was dried with a vacuum drier and pulverized to obtain 30 g of Composition 2.

Then, the composition 2 was suspended in 20 times as much distilled water and extracted three times with the same amount of ethyl acetate solvent to obtain an ethyl acetate fraction. The ethyl acetate fraction was concentrated under reduced pressure, and the concentrate was dried with a vacuum drier and pulverized to obtain 10 g of Composition 3

The compositions 1 to 3 prepared above were loaded onto liquid chromatography to proceed with compound separation. The compounds thus obtained were identified as the compounds of the formulas (1) to (12) using the NMR spectroscopic analysis apparatus. The composition prepared above was dissolved in 60% by volume methanol, filtered through a 0.2 μm membrane filter, and loaded into high performance liquid chromatography. In high performance liquid chromatography, HP ODS Hypersil was used as the column, distilled water and methanol were used as the solvent, and the solvent was fed from a linear gradient of from 15 vol% to 70 vol% of methanol for 30 minutes at a flow rate of 1.0 ml / ). As a result, the presence of the compounds of Formulas 1 to 12 was confirmed. The chemical composition of the composition is shown in Table 1 below.

sample Example 1 Composition 1 20% by weight of total fluorotannine containing 2% by weight of the formula 2, 1% by weight of the formula 4, 0.5% by weight of the formula 5, 0.3% by weight of the formula 8 and 16.2% by weight of the single compound of the formula 1-12, , 20% by weight of alginic acid, 20% by weight of fucoidan, 5% by weight of fucosterol, 5% by weight of fucoxanthin, 5% Composition 2 70% by weight of total fluorotannine, 10% by weight of mannitol, 3% by weight of the compound of formula (2), 3% by weight of the compound of formula (4) , 6% by weight of alginic acid, 6% by weight of fucoidan, 3% by weight of fucosterol, 3% by weight of fucozanthin, 2% Composition 3 100% by weight of total fluorotannin including 17% by weight of Formula 2, 5% by weight of Formula 4, 5% by weight of Formula 5, 3% by weight of Formula 8, and 70%

Example 2. Preparation of Powdered Product Example 1 Using the Composition

To prepare a powdered product utilizing the composition of Example 1, Example 1 was prepared by combining collagen, flaxseed extract, calcium, iron, vitamin B12, vitamin E, and copper. The composition of the product example 1 is shown in the following [Table 2]. The manufactured product example 1 is shown in Fig.

Ingredients content(%) Composition 2 5 Colaken 75 Flaxseed extract 4 calcium 15 iron content 0.7 Vitamin B12 0.01 Vitamin E 0.2 Copper 0.09 Sum 100

Product Example 1 itself can be fed to animals and mixed with common feed. It can also be used easily with snacks such as canned foods and chicken breasts.

Example 3 Preparation of Product Example 2

Animal feeds (Product Example 2) were prepared by adding other raw materials to the above-mentioned Product Example 1, and the composition thereof is shown in Table 3 below. Product Example 2 can be fed in the same manner as ordinary feeds. Feed amount is 1.5 ~ 3g per animal, 2.5 ~ 5g for animals less than 15 ~ 30kg and 5 ~ 7.5g for animals over 30kg. The prepared animal feed is shown in [FIG. 2], [FIG. 3].

Ingredients content(%) Product Example 1 8 Hydrolyzed chicken 6 Pig gelatin 5 Rice flour 33 Soybean flour 15 flour 4 Vitamins, minerals 0.1 Chicken oil 3 Sorbitol, propylene glycol 17.3 glycerin 2.6 fish 2 water 4 Sum 100

Experimental Example 1. Evaluation of antioxidative effect of composition

The antioxidative effect of the composition of Example 1 was evaluated as follows.

After dissolving the composition of Example 1 and vitamin C known to have an antioxidant effect at a concentration of 1 mg / mL in 50% ethanol aqueous solution, respectively, at a 10% ratio in a DPPH (1,1-diphenyl-2-picrylhydrazyl) solution having an absorbance of 0.94 to 0.98 Leave for 10 minutes after addition. The absorbance is measured after 10 minutes to calculate the radical scavenging rate. The method of calculating the radical scavenging rate is as follows.

Radical scavenging rate (%) = (initial absorbance - absorbance after 10 minutes) / initial absorbance X 100

The results of Experiment 1 are shown in [FIG. 4]. As shown in FIG. 4, the composition of the present invention has an antioxidant activity equivalent to that of vitamin C.

Experimental Example 2. Evaluation of anti-inflammatory effect of the composition

To evaluate the anti-inflammatory effect of the composition on animals, the macrophage cell line (RAW 264.7) in rats was treated with LPS (lipopolysaccharide) and the NO, PGE2 and COX-2 levels were measured to evaluate the anti-inflammatory effect.

Measurement of NO concentration was pretreated with the composition of Example 1 to the culture cells by concentration (30 ~ 120μg / mL) 1 hour after the LPS treatment to induce an inflammatory response. After 24 hours of LPS treatment, the culture solution was mixed with an equal amount of a Griess reagent and absorbance was measured at 530 nm. For the NO concentration calculation, a standard curve was created using the absorbance of NaNO ₂ concentration, and the concentration was calculated.

The concentrations of PGE2 and COX-2 were measured using an enzyme-linked immunosorbent assay (ELISA assay). The cell culture medium was inoculated into the enzyme-coupled immunosorbent kit one day after the treatment with LPS and the composition, and the absorbance was measured at 540 nm. Concentrations of each protein were calculated by preparing a standard curve with a 4-PL logistic curve fit using absorbance results obtained by treating standard products in concentration-dependent manner. The anti-inflammatory effect evaluation results are shown in [FIG. 5] to [FIG. 7]. The significance of the test results was confirmed by using T-test and P <0.05 (* P <0.05 vs control, P <0.05 vs LPS).

5 to 7 as shown in the experimental results of the composition of the present invention can be confirmed that significantly reduces the inflammatory response caused by LPS.

According to Figure 5, when the treatment of the composition of the present invention with LPS in the experiment using the macrophages of rats it can be seen that the concentration of NO decreases and the higher the concentration, the more the concentration of NO is further reduced. have. This means that the composition of the present invention reduces inflammation by inhibiting the production of NO, an inflammatory reaction product caused by LPS.

According to Figure 6, when the treatment of the composition of the present invention with LPS in experiments using rat macrophages can confirm that the concentration of COX-2 is reduced, the higher the concentration of COX-2 is further reduced I can see that. This means that the composition of the present invention reduces inflammation by inhibiting the production of COX-2, an inflammatory response protein caused by LPS.

According to FIG. 7, when the composition of the present invention is treated with LPS in experiments using rat macrophages, it can be seen that the concentration of PGE2 decreases and that the concentration of PGE2 decreases further as the concentration increases. have. This means that the composition of the present invention suppresses inflammation by inhibiting the production of PGE2, an inflammatory response protein caused by LPS.

Experimental Example 3. Evaluation of efficacy of Product Examples 1 and 2

A sample prepared by taking 0.1 g of the powder of Product Example 1 in the form of a capsule and the sample of Product Example 2 were continuously fed to the experimental animals together with feed for one month. The efficacy of the product samples was evaluated by evaluating the smell, color change, odor and hair gloss of the animals before and after the feeding. The evaluation method was evaluated as 2 (bad), 4, 6, 8, 10 (good) using the 5-point scale. For the objective test results, only the animals that did not have any abnormality in health were selected and the experimental animals were opened. According to the weight of the experimental animals, in Example 1, 1 capsule was administered to animals less than 15 kg, 2 capsules for animals less than 15 to 30 kg, and 3 capsules for animals over 30 kg were fed twice a day. In Product Example 2, 1.5 g of the animal less than 15 kg, 2.5 g of the animal less than 15 to 30 kg, and 5 g of the animal more than 30 kg were fed twice daily with the feed. The evaluation of the animals fed the product examples 1 and 2 is shown in the following [Table 4].

As shown in the results of the table, it was confirmed that the product examples 1 and 2 of the present invention improved the gloss, smell, odor, and color of the hairs of the fed animals.

division Efficacy assessment (the higher the score, the better) Gloss of hair smell Odor Stained color Before pay After salary Before pay After salary Before pay After salary Before pay After salary Product Example 1 5.6 8 5.6 8.8 4.6 7.8 5.6 7.4 Product Example 2 5.4 8 5.6 8.6 4.2 6.6 5.6 7.2

Experimental Example 4. Observation of Obesity Improvement Efficacy of Product Example 2

In order to evaluate the efficacy of the animal feed of Example 2 for improving obesity, 10 dogs that were overweight by 20% of their normal weight were selected and fed with the product Example 2 when they were fed. In Example 2, 1.5 g of the animal less than 15 kg, 2.5 g of the animal less than 15 to 30 kg, and 5 g of the animal more than 30 kg were fed twice a day, and the obesity improvement effect was evaluated by comparing the post- Respectively. For the objective test results, the animals were selected from the animals that had undergone growth, and those without obesity were selected. The overweight of the animals was calculated by the following formula using the standard weight per breed.

Overweight (%) = (current weight - normal weight) / normal weight

The results of the evaluation of the obesity-improving efficacy of the product Example 2 are shown in Table 5 below.

As shown in the experimental results of the table, it was confirmed that the obesity of the dog fed with the product Example 2 of the present invention was improved.

division Overweight before pay (%) Overweight after pay (%) Product Example 2 20.69 8.17

Claims (13)

Animal feed composition comprising a phlorotannin compound as an active ingredient
The composition of claim 1, wherein the phlorotannin compound is at least one selected from phlorotannins represented by the following Chemical Formulas 1 to 12:
[Chemical Formula 1]

(2)

(3)

[Chemical Formula 4]

[Chemical Formula 5]

[Chemical Formula 6]

(7)

[Chemical Formula 8]

[Chemical Formula 9]

[Formula 10]

(11)

[Chemical Formula 12]

The composition according to claim 1, wherein the composition comprises 20 to 100% by weight of a total phlorotannin compound The composition for animal feed according to claim 1, wherein the composition comprises at least one kind of phlorotannin selected from the group consisting of formulas (2), (4), (5) and The composition of claim 4, wherein the composition comprises 1 to 60 wt% of a fluorotannine of Formula 2, 0.3 to 20 wt% of a fluorotannine of Formula 4, 0.1 to 10 wt% of a fluorotannine of Formula 5, 0.1 to 5% by weight of an animal feed composition The animal feed composition according to claim 1, wherein the animal feed composition is an algae extract The method of claim 1, wherein the fluorotannine compound is selected from the group consisting of Eisenia bicyclis, Eisenia arborea, Eisenia desmarestioides, Eisenia galapagensis, (Ecklonia radiata), Eclonia bacillus (Ecklonia spp.), Ecklonia cava, Ecklonia stolonifera, Sea bamboo (Ecklonia maxima), Ecklonia radiata, Ecklonia bicyclis), Ecklonia biruncinate, Ecklonia buccinalis, Ecklonia caepaestipes, Ecklonia exasperta, Ecologia pasty, Ecklonia fastigiata, Ecklonia brevipes, Ecklonia arborea, Ecklonia latifolia, Ecklonia latifolia, wherein the composition is extracted from at least one kind of seaweed selected from the group consisting of lonia muratii, Ecklonia radicosa, Ecklonia richardiana and Ecklonia wrightii. The method of claim 3, wherein the composition comprises 20 to 100% by weight of fluorotannine, 0 to 30% by weight of mannitol, 0 to 25% by weight of alginic acid, 0 to 25% by weight of fucoidan, 0 to 10% by weight of fucosterol, 0 to 10% And 0 to 10% by weight of laminaran. Animal product comprising the composition of claim 1 10. An animal product according to claim 9, characterized in that the product is in the form of animal feed, canned food for animals or gum The animal product according to claim 9, wherein the product comprises 0.1 to 50% by weight of the composition, based on the total weight of the product. [Claim 11] The animal product according to claim 9, wherein the product further comprises at least one member selected from the group consisting of collagen, flaxseed extract, calcium, iron, vitamin B12, vitamin E and copper The method according to claim 9, wherein the product is 1 to 50% by weight of fluorotannin composition, 40 to 80% by weight of collagen, 1 to 6% by weight of flaxseed extract, 0.001 to 18% by weight of calcium, 0.1 to 1% by weight of iron, vitamin B12 0.00001 ~ 0.01% by weight, 0.1-0.5% by weight of vitamin E and 0.05-0.1% by weight of copper

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