KR20230054016A - Antiseptic composition and process for preparing the same - Google Patents

Abstract

The present invention relates to an antiseptic composition comprising a Lespedeza cuneata extract for feed and a method for preparing the same and, more specifically, to: an antiseptic composition comprising a Lespedeza cuneata extract for an animal, which minimizes side effects and irritation, inhibits the growth of germs, and enhances the absorption by animals, preservation of feed, and safety for workers to result in eco-friendly and economical performance; and a method for preparing the same. The antiseptic composition for animal feed according to the present invention, as a natural preservative prepared using plant extracts in an appropriate mixing ratio, gives no irritation to be eco-friendly and inhibits the propagation of various germs without reducing the animals' ability to eat, thereby enhancing the preservation and safety of feed such that a pleasant working environment is provided for workers and surroundings of the workers and death due to hyperacidity can be reduced to bring economic advantages.

Description

Preservative composition for feed and method for preparing the same {Antiseptic composition and process for preparing the same}

The present invention relates to a preservative composition for feed and a method for manufacturing the same, and more particularly, by minimizing side effects and irritants, inhibiting the propagation of various germs, and increasing animal absorption, feed preservability, and safety for workers, which are environmentally friendly. It relates to a preservative composition for animals containing an economical night gate extract and a method for preparing the same.

Preservatives are agents that prevent decay of materials, and preservatives are agents that prevent decay of animal and vegetable organic matter by the action of microorganisms, and agents that are added to preservatives for the purpose of preservation are preservatives. Microorganisms that cause spoilage include molds and yeasts belonging to fungi, and bacteria, which are lower microorganisms.

Preservatives are essential to maintain the quality of various product groups such as food, cosmetics, pharmaceuticals, and household goods for a long period of time, and must not be harmful to the human body and must not damage product quality due to the addition of preservatives.

Conventionally, chemically synthesized preservatives have been generally used in food and medicine. For example, chemical preservatives such as calcium propionate, sodium benzoate, sodium nitrite, and sorbic acid have been used as food preservatives, and methyl paraben, ethyl paraben, and propyl as cosmetic preservatives. Alkyl parabens such as propyl paraben and butyl paraben, and chemical synthetic preservatives such as quaternium-15, imidazolidinyl urea, and phenoxyethanol were used.

Since these chemical synthetic preservatives are very harmful to the human body or livestock when the amount is exceeded, their use is regulated by the Pharmaceutical Affairs Act or the Food Sanitation Act. Therefore, attention is being focused on the development of preservatives that can strongly prevent deterioration and decay of products caused by microorganisms without causing toxicity or side effects to the human body, and in particular, research to develop them from natural products is being actively conducted.

However, formulations made of natural materials are known to be less toxic and irritating to the human body, highly biodegradable and environmentally friendly, whereas the natural preservatives developed so far have problems such as low economic feasibility, narrow antibacterial spectrum or limited range of use due to physical properties. has

Accordingly, it is necessary to develop a natural preservative that is harmless to the human body and rich in functionality.

Korean Publication No. 10-2019-0090715 (August 02, 2019) Korean Publication No. 10-2020-0050121 (May 11, 2020) Korean Registration No. 10-0983791 (September 16, 2010) Korean Patent Registration No. 10-1710589 (February 21, 2017)

The inventors of the present invention have studied intensively using natural materials to develop an animal preservative composition that is less irritating, eco-friendly and economical, and improves the problems according to the prior art as described above. As a result, the preservative composition described below can satisfy the above requirements. and came to complete the present invention.

Therefore, an object of the present invention is to provide a preservative composition for feed, which is improved in problems according to the prior art, is less irritating, environmentally friendly and economical.

The purpose of the present invention as above is

Night gate extract 60-80 parts by weight, Hwangchil extract 9-24 parts by weight, Cypress extract 3-7 parts by weight, Coconut extract 3-7 parts by weight, Potassium sorbate 0.1-2.0 parts by weight, Vitamin E acetate 0.1-1.5 parts by weight, and It can be achieved by a preservative composition for animal feed comprising 0.1 to 0.3 parts by weight of sea salt and a method for producing the same.

The preservative composition for animal feed according to the present invention is a natural preservative prepared by using a plant extract of an appropriate mixing ratio, and is non-irritating, so it is eco-friendly, does not reduce the feeding power of animals, and inhibits the propagation of various germs, thereby increasing the preservability and safety of the feed. And it can provide a pleasant working environment around the living, and provide an economic advantage of reducing mortality due to overacidosis.

1 is a manufacturing process diagram for a preservative composition for animal feed according to the present invention.

The present invention, in one aspect,

Night gate extract 60-80 parts by weight, Hwangchil extract 9-24 parts by weight, Cypress extract 3-7 parts by weight, Coconut extract 3-7 parts by weight, Potassium sorbate 0.1-2.0 parts by weight, Vitamin E acetate 0.1-1.5 parts by weight, and It provides a preservative composition for animal feed comprising 0.1 to 0.3 parts by weight of sea salt and a method for producing the same.

Hereinafter, a preservative composition for animal feed according to the present invention and a manufacturing method thereof will be described in more detail with reference to the accompanying drawings.

The terms or words used in this specification and claims are not limited to the usual or dictionary meanings, and the inventor can properly define the concept of the term in order to explain his or her invention in the best way. Based on this, it should be interpreted as a meaning and concept consistent with the technical spirit of the present invention. Therefore, since the embodiments described in this specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention, it is understood that there may be various equivalents and modifications that can replace them at the time of this application. shall.

In the present invention, the antiseptic composition for animals is expressed in parts by weight or % by weight, and the sum of the individual components may include an unpracticable part exceeding 100% by weight according to the calculation formula, but such excess or unpracticable part It is obvious that it is not included in the scope of the invention, and in particular, an amendment to clarify this in the claims, for example, an amendment to add a proviso such as the range exceeding 100% by weight is excluded from the scope of the present invention is unclear. It is only to clarify one description, and it is obvious that it does not affect the nature or scope of the present invention.

Conventional feed additives are manufactured by containing chemicals such as strong acid propionic acid as a preservative, so that they are highly irritating and reduce the feed intake rate of livestock, often causing animal mortality due to overacidosis, and livestock handling them Odors and stimuli on the human body of workers not only caused side effects that could even cause blindness in severe cases, but also caused civil complaints from neighbors due to the strong pungent odor. Provides an eco-friendly and economical animal preservative composition by providing a preservative composition for animals that minimizes the above side effects and irritation, inhibits the propagation of germs, and increases the absorbency of animals, the preservation of feed, and the safety of workers. It has technical features to do, which can provide various benefits in an environmentally friendly way.

As shown in FIG. 1, the manufacturing method of the preservative composition for animal feed according to the present invention largely includes a pretreatment step of plant materials, a blending step, an extraction step, an additive mixing step, a formulation step, and a packaging step. The individual steps are described in detail below.

a) Pre-treatment of plant material

First, plant materials obtained through various routes are preferably washed with a high-pressure sprayer using purified water or the like to facilitate processing in a subsequent step, and then pre-treated by filtering out dust and the like. The plant materials include night gate, hwangchil tree, cypress, and coconut fruit.

b) mixing step

As a step of blending the plant material at a certain ratio, the mixing ratio of the plant material is 60 to 80 parts by weight of night gate extract, 9 to 24 parts by weight of hwangchil extract, 3 to 7 parts by weight of cypress extract and coconut based on 100 parts by weight of the total composition. It is preferable that the extract is 3 to 7 parts by weight.

The night gate ( Lespedeza cuneata ) is mainly distributed in Korea, Japan, Taiwan, India, Australia, etc., and is also called Bisuri, Nougeun, Hojija, and Sanchaeja. The night gate is bitter in taste, slightly bitter in nature, non-poisonous, and mainly acts on the lungs, liver and kidneys. It is known to be effective in improving functions of the kidneys, liver, intestines, etc., and in improving blood circulation. It removes stagnation of blood and lowers swelling. It has been reported to improve blood circulation. It has also been reported to be effective in lowering fever, killing insects in the stomach, treating boils in the breast, snake bites, red bloodshot eyes, and stomach ulcers and prolapse. There are pinitol, flavonoid, tannin, and bisitosterol in the leaves, roots, and stems of the night gate. , has the effect of killing or suppressing catarrhal cocci, etc. The night gate can use leaves, stems, and roots.

In addition, Hwangchil ( Dendropanax morbifera Lev. ) is an evergreen broad-leaved arboreous tree belonging to the Araliaceae Hwangchil genus, and is a native Korean species that grows up to 15m or more in height. The leaves are alternate, and the shape is not regular in five shapes depending on the amount of sunlight. Young branches are green and glossy, flowers bloom in June, and 7-19mm long fruits ripen black. It is a temperate plant that grows in areas where the minimum temperature is over -2℃ and the annual average temperature is over 12~15℃. Worldwide, Hwangchil is a rare species that grows only in the inland and coastal areas of the southwestern part of the Korean Peninsula, islands, and Jeju Island, and its quantity is currently extremely limited. Currently, island regions include Sinan, Jindo, Wando, and Jeju Island, and coastal regions include Haenam, Jangheung, Gangjin, Goheung, Seungju, Gwangyang, and Yeosu. In addition, the clove component contained in hwangchil has not only the value as a benzoin that makes the mind and body clear and comfortable, but also has various pharmacological actions, so it has more value as a perfume, functional food or beverage, or medicine rather than as a paint. Hwangchil can use leaves, stems, branches, etc.

Hinoki Cypress ( Chamaecyparis obtusa ) is an evergreen tree in the Araceae family, and is also called hoemok, hinoki and cypress. The height is 30-40 m, the width is about 1-2 m, the bark is reddish brown, and small needle-shaped leaves grow densely on the branches. Distributed in Japan and southern Korea, ingredients include γ-cadinene, α-terpineol, and borneol, and leaves and wood contain 1% essential oil. . Cypress enhances immunity in the body by generating antibodies against antigens, and is widely used as a natural insecticide to combat house dust mites that cause allergic diseases such as atopy, asthma and rhinitis. It is good to use cypress leaves, stems, or branches extracted. The cypress extract may be used alone or in the form of a mixture. Cypress can use leaves, stems, branches, etc.

Coconut is scientifically named Cocos nucifera L , and is also called the coco palm. The fruit of the palm, a palm plant native to South America. It grows in India and the West Indies. There are trees of 15-30m in height and half that height, and the fruit size is 25-30cm in length and 20-25cm in diameter in an egg shape, and the fruit is opened at the top of the tree. The main production areas are Thailand, the Philippines and Indonesia.

Coconut fruit is juicy and is drunk as a beverage. The jelly-like flesh inside the fruit is eaten as it is or used to make oil. The fiber layer surrounding the fruit is used to make carpets, industrial ropes, car seats, etc. Use as supplies or craft materials. It may be desirable to use coconut by removing the liquid in the center of the fruit and extracting the remaining pulp.

Since the antiseptic composition for animals according to the present invention contains an extract of a natural substance rather than a chemical synthetic agent, as can be seen from the results of the following examples and test examples, it has excellent stability due to no irritation or toxicity to the skin. Since it has functionality such as antioxidant properties and preservative effect, it can be effectively used as a preservative or additive for feed requiring such functionality.

The desired effect in the present invention is to maximize the functionality including antioxidant effect and preservative effect by combining the above-mentioned natural ingredients in an optimal ratio in consideration of the effective dose and side effects of each component, and In some cases, there is a risk of ineffectiveness.

The night gate has weak antioxidant and preservative functions at 60 parts by weight or less based on 100 parts by weight of the total formulation, and it is difficult to properly harmonize with other ingredients at 80 parts by weight or more. The hwangchil has weak anti-inflammatory properties and neutralizing function at 9 parts by weight or less, and economical efficiency becomes a problem at 24 parts by weight or more because there is no synergistic effect according to the amount added. Cypress has a weak clove function at 3 parts by weight or less, and economical efficiency becomes a problem at 7 parts by weight or more. Coconut extract is included in 3 to 7 parts by weight, so that anti-inflammatory effect, moisturizing effect, stable harmony of the overall formulation and various functions can be optimally exhibited.

c) extraction of plant material

Then, the mixed plant material is extracted with alcohol to obtain an extract.

Alternatively, each plant material may be individually extracted and then appropriately mixed and used within the range set above. In the present invention, in order to simplify the procedure, for convenience, a method of mixing and extracting the plants will be mainly described.

The mixed extract of the night gate, hwangchil tree, cypress and coconut is preferably obtained by leaching at room temperature for 2 to 4 months using alcohol, and the alcohol has an alcohol concentration of 35 to 95% (v / v) ethanol It is more preferable that it is diluted alcohol.

Alternatively, the plant materials may be subjected to hot water extraction, chilling or warming extraction by adding an extraction solvent. In this case, the extract can be obtained by adding and mixing 2 to 20 times the extraction solvent by weight with respect to the plant material, extracting at 70 to 125 ° C. for 2 to 24 hours, filtering, and concentrating.

Here, as the extraction solvent, at least one selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, alcohols, or mixtures thereof may be used. Methanol, ethanol, etc. can be used as a lower alcohol having 1 to 4 carbon atoms, and butylene glycol, propylene glycol, pentylene glycol, etc. can be used as a polyhydric alcohol. As the mixture, a mixture of water and a lower alcohol, a mixture of water and a polyhydric alcohol, a mixture of a lower alcohol and a polyhydric alcohol, or a mixture of water, a lower alcohol and a polyhydric alcohol may be used.

Filtration may be performed by, for example, filtering out solid foreign substances and particles using a filtration membrane, paper filter paper, hemp, nonwoven fabric, or cotton, or by using an ultrafiltration method, a cryofiltration method, a centrifugal separation method, or the like. In addition, filtration may be performed in a manner in which primary filtration is performed using gauze and secondary filtration is performed using Whatman paper.

The filtrate can then be used in a subsequent step as a liquid, preferably by subsequent evaporation to obtain an extract for the production of a solid product.

d) additive mixing step

To the plant extract, additives useful for preservative function, for example, 0.1 to 2.0 parts by weight of potassium sorbate, 0.1 to 1.5 parts by weight of vitamin E acetate, and 0.1 to 0.3 parts by weight of sea salt are added and mixed.

In addition, a small amount of separate additives may be added to prevent deterioration of quality or to increase functionality, and examples of such additives include preservatives, anticoagulants, binders, emulsifiers, antioxidants, antifungal agents, flavoring agents, and the like. .

Antifungal agents include glyceryl caprylate (GMCY), 4-hydroxyacetophenone, 1,2-hexandiol, ethylhexyl glycerin, and the like.

e) formulation step

Then, the mixture mixed with the additives can be formulated into a certain shape, for example, the mixture is diluted (eg, 5 to 20 times) with purified water and formulated into a liquid product or has an average diameter of 1 to 20 mm. It can be formulated by aging and drying at room temperature for 7 to 20 days so that the moisture content of the mixture is 3 to 15% after molding with a ventilation device.

Then, the formulated antiseptic composition is sterilized by a conventional method to improve the preservation of the product, and is usually sterilized at 90 to 125 ° C. for 5 to 60 minutes to kill microorganisms.

f) packaging step

Finally, the product of the animal preservative composition that has undergone the sterilization process is packaged in a certain unit and stored in storage until shipment.

The preservative composition for animals may be preferably mixed and fed at 1 to 10% by weight based on the weight of the formulated feed.

The compounded feed can be used without any special restrictions, and commercially available general compounded feeds, red clay, green barley silage, brewer's meal, pearl barley, wheat hull, rice bran, cottonseed, wheatgrass, molasses, yeast culture, minerals and vitamins, minerals, and salt are used. may include

In addition, the formulated feed may more preferably contain 1 to 10% by weight of the animal preservative, 10 to 40% by weight of rice bran, 10 to 40% by weight of wheat bran, 10 to 40% by weight of grain, and 1 to 3% by weight of salt. can

The preservative composition for animals thus prepared has no strong odor, has excellent antibacterial action, and is rich in calories and nutrients, so that the safe growth of livestock can be promoted, and it can be used in combination with formulated feed, cattle, It can be usefully used not only for raising livestock such as pigs, sheep, goats, ducks, geese, and chickens, but also for fish farming. In addition, it can be used as a feed for companion animals such as dogs and cats or edible insects.

<Example>

Hereinafter, the present invention will be described in more detail by way of examples. However, these examples are described to aid understanding of the present invention and do not limit the content and scope of the present invention.

Statistical analysis: All analysis values obtained in the test were presented as average values for each treatment group. Statistical analysis was performed using GLM (General Linear Model) of SAS (SAS Institute, 1996). Treatment is the main effect, and cow shed is the experimental unit for the analysis of all parameters. The efficacy of the other steps was also evaluated. The significance test was analyzed using Duncan's multiple range test for statistical analysis.

Example 1 to 6: Preparation of antiseptic composition for animals

The night gate, hwangchil, cypress and coconut are washed and pretreated, then mixed as shown in Table 1 below, then leached at room temperature for 3 months using 50% diluted alcohol to obtain an extract, then mixed with additives, After sterilization, it was subdivided into 10 Kg each to prepare a liquid preservative for animals.

ingredient Content (weight ratio) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 night gate 60 65 70 70 75 80 35 Hwangchil 24 24 20 18 15 9 30 cypress 7 6 4.5 6.5 3 3 33 coconut 7 4 4.5 3 5.1 6.5 - potassium sorbate One 0.8 0.2 2.0 0.1 One - Vitamin E Acetate 0.9 0.1 0.6 0.3 1.5 0.3 - sea salt 0.1 0.1 0.2 0.2 0.3 0.2 - propionic acid - - - - - - 2 Sum 100 100 100 100 100 100 100

Example 7-12: Manufacture of formulated feed

The preservative for animal feed of Example 3 was selected as a representative test group to prepare the following formulated feed.

ingredient Content (Kg) Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Comparative Example 2 rice bran 18 14 10 33 40 38 50 Mackang 40 40 40 35 10 40 30 grain 40 40 37 20 37 10 19 antiseptic One 5 10 10 10 9 - salt One One 3 2 3 3 One Sum 100 100 100 100 100 100 100

Test Example 1: DPPH antioxidant effect measurement

DPPH free radical scavenging experiments were performed on the samples of Examples 1 to 6 and Comparative Example 1. DPPH (1,1-diphenyl-2-picrylhydrazyl) itself is a very stable free radical. It measures a deep purple compound that shows specific light absorption at 517 nm, and is an antioxidant with radical scavenging activity. Antioxidant activity can be easily measured due to quantitative decolorization by Since the scavenging activity by these radicals shows a correlation with antioxidant activity including lipid peroxidation inhibitory activity, it is widely used in antioxidant search. Antioxidant activity measurement (Electron donating abilities, EDA) of the sample was measured by modifying the method of Blois. 0.5 ml of 0.2 mM DPPH (1,1-diphenyl-2-picrylhydrazyl) was added to 20 ml of each sample solution, stirred, allowed to stand for 30 minutes, and then absorbance was measured at 517 nm. The electron donating effect was expressed as the rate of decrease in absorbance between the added and non-added groups of the sample solution. The resulting free radical scavenging ability was calculated by the following formula.

Free radical scavenging activity (%) = {1-(B/A)}×100

In the formula, A: Absorbance of the control wells not treated with the sample, B: Absorbance of the experimental group wells treated with the sample. The results are shown in Table 3.

Ex1 Ex2 Ex3 Ex4 Ex5 Ex6 Comp. Ex1 DPPH free radical-scavenging activity (%)
87.33
85.52
88.17
86.25
84.70
82.35
52.32

Through the above results, it was confirmed that the samples of Examples 1 to 6 generally exhibited superior DPPH free radical scavenging activity compared to those of Comparative Examples.

Test Example 2: Antibacterial activity test

The antiseptic and sterilizing power of the compositions according to Examples 1 to 6 against microorganisms was tested by conducting a disc paper test.

The test strain was dispensed into a petri dish, spread widely over the entire medium using a glass rod, and then a paper disc with a diameter of 5 mm was placed on it. And 30 μl of the composition of each example was placed on a paper disc. After culturing overnight at 30 ° C., the inhibition zone was checked to determine antibacterial activity. The results are shown in Table 4 below.

test strain Inhibition zone(d) : ㎝ Ex1 Ex2 Ex3 Ex4 Ex5 Ex6 Comp. Ex1. Staphylococcus aureus
ATCC 10537 2.7 2.6 2.9 2.9 2.7 2.7 1.2 Staphylococcus aureus
ATCC 256E 2.7 2.7 2.9 2.9 2.7 2.6 1.4 Salmonella typhimurium
KCTC 1925 2.7 2.8 3.1 3.1 2.8 2.7 1.7 Candida albicans
ATCC 10231 2.7 2.8 2.9 3.1 2.7 2.6 2.1

As can be seen from the above test results, the antiseptic composition according to the present invention has an excellent antibacterial action against the test bacteria, although there is a difference in degree, so it can be used as an additive for feed for applications requiring it.

Test Example 3: Measurement of growth inhibitory activity of mold

Fungi, Trichophyton rubrum (KCTC 6345), Mycosporum audouinii (KCTC 6346), Trichophyton ferrugineum (KCTC6351), Epidermophyton floccosum , KCTC 6586) and Trichophyton mentagrophytes ( Trichophyton mentagrophytes , KCTC 6077) were tested for growth inhibitory activity. The fungal growth inhibitory activity was assayed by the paper disc diffusion method. After inoculating each strain on Sabouraud's agar medium, each composition (Examples 1 to 6, treatment concentration: 100 μg) was directly treated on a paper disc. After placing the litchi on the medium inoculated with each bacteria and incubating it aerobically for 5 days at 28 ° C, the antibacterial activity was checked by looking at the size of the clear zone formed around the litchi, and the results are shown in Table 5 was

test strain Inhibition zone(d) : ㎝ Ex1 Ex2 Ex3 Ex4 Ex5 Ex6 Comp. Ex1. Trichophyton rubrum 3.0 3.1 3.4 3.2 3.2 3.2 0.7 Micosporum audouinii 3.1 3.1 3.4 3.2 2.8 2.9 0.8 Trichophyton ferrugineum 2.9 3.0 3.4 3.3 2.9 3.0 0.9 Epidermophyton floccosum 2.9 3.2 3.5 3.2 2.8 2.8 1.1 Trichophyton mentagrophytes 3.1 3.2 3.5 3.4 3.0 2.8 0.9

The size of the growth-inhibiting ring by the composition of the examples was 2.8 cm or more, and it was found to have excellent fungal growth inhibitory activity. This antibacterial activity showed a similar pattern to the rest of the fungi.

Test Example 5: Acute toxicity test

An acute toxicity test was performed on the compositions of Examples 1 to 6, and the presence or absence of toxicity was observed. As experimental animals, 60 male and 95 female SD-based rats aged 4 and 5 weeks and weighing 105 were used and tested using the composition of each example and distilled water as a negative control. First, the rats were placed in a laboratory breeding box with a temperature of 22° C., a relative humidity of 53%, a light-dark cycle of fluorescent lighting (09:00 on-18:00 off), and an illuminance of 150-300 Lux for a period of about 1 week. After acclimatization over the period, only healthy animals were selected, divided into groups to match the average body weight, and forced orally administered once a day in an amount of 20 ml / kg for 14 days. , autonomic nerves, weight changes, and the presence or absence of dead animals were examined.

According to the experimental results, there was no significant change in body weight during the experimental period, but there was no significance, and no dead animals were observed even though the highest concentration of 20ml per kg was selected for the amount of sample, which is the maximum amount that can be administered to test animals. It was not possible to calculate the lethal dose, so the LD ₅₀ was found to be 20ml/kg BW or more, and no specific general symptoms or specific lesions were observed at autopsy.

Test Example 6: Breeding pigs according to feed feeding

As shown in Table 6, 70 sows with an average weight of 60 ± 3 kg were tested in 7 groups for the development of high quality such as productivity and meat quality characteristics of pigs by feeding according to Examples 7 to 12 and Comparative Example 2 They were isolated in groups and reared for 60 days in groups of 10 each. The analysis of the test feed was conducted according to the AOAC (1996) method, and feed and water were freely consumed during the feeding test.

For the test group, the body weight and feed intake rate of pigs fed individual feed were investigated. The starting weight was measured on the test start date and the end weight was measured on the test end date. The weight gain is the value obtained by subtracting the starting weight from the final weight, and the daily weight gain is the value obtained by dividing the weight gain by 60 days of the total specification test. For feed intake, the remaining amount of feed fed the previous day was measured every morning before feeding, and feed conversion rate was determined by dividing feed intake by daily weight gain. The results are shown in Table 6 below.

Test Items Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Comparative Example 2 Starting weight (Kg) 60.60±0.31 60.45±0.11 60.66±0.72 61.30±0.71 61.25±0.23 60.33±0.45 60.14±1.25 End Weight (Kg) 112.34±0.42 113.32±0.68 113.35±1.21 110.34±1.12 111.35±0.27 109.55±0.36 92.37±1.25 Weight gain (Kg) 51.74±0.05 52.87±0.35 52.69±0.53 49.04±0.87 50.01±0.06 49.22±0.11 32.23±0.12 Weight gain per day (g) 882.33±0.46 881.16±1.32 878.16±1.32 817.33±2.31 833.50±2.15 820.03±2.36 537.16±2.65 Feed intake (g) 2,180
±2.50 2,180
±2.50 2,180
±2.50 2,180
±2.50 2,180
±2.50 2,180
±2.50 2,180
±2.50 feed conversion rate 2.47±0.21 2.47±0.40 2.48±0.36 2.66±0.48 2.46±0.51 2.65±0.28 3.49±0.33

As a result of the experiment, as shown in Table 6, the final weight, weight gain, and daily weight gain were significantly higher (P<005) in the feeding group of the example than in the control group. There was no significant difference in feed intake. When looking at these results, it was confirmed that the feeding of the feed according to Examples 7 to 12 increased the amount of weight gain due to the increase in feed intake.

In this way, the present invention has been described with examples of preferred embodiments of the present invention through the drawings and specifications, but it is obvious that various modifications and equivalent implementations are possible for those skilled in the art.

Claims (4)

Night gate extract 60-80 parts by weight, Hwangchil extract 9-24 parts by weight, Cypress extract 3-7 parts by weight, Coconut extract 3-7 parts by weight, Potassium sorbate 0.1-2.0 parts by weight, Vitamin E acetate 0.1-1.5 parts by weight, and A preservative composition for animal feed comprising 0.1 to 0.3 parts by weight of sea salt. The method of claim 1,
The extract is obtained by leaching at room temperature for 2 to 4 months using alcohol, and the alcohol is a preservative composition for animal feed, characterized in that 35%-95% alcohol. a) washing the plant material and then pre-treating it to filter out foreign substances;
b) blending the plant material in a certain ratio;
c) extracting the mixed plant material with alcohol to obtain an extract;
d) mixing the obtained extract with an additive to obtain a mixture;
e) formulating the mixture into a predetermined shape; and
f) packaging the formulated product in a predetermined unit; includes,
The plant material includes 60 to 80 parts by weight of night gate extract, 9 to 24 parts by weight of hwangchil extract, 3 to 7 parts by weight of cypress extract, and 3 to 7 parts by weight of coconut extract, and the additives include 0.1 to 2.0 parts by weight of potassium sorbate, A method for producing a preservative composition for animal feed, characterized in that 0.1 to 1.5 parts by weight of vitamin E acetate and 0.1 to 0.3 parts by weight of sea salt. The method of claim 3,
The extraction step is performed by leaching the mixed plant material at room temperature for 2 to 4 months using alcohol, and the alcohol is a method for producing a preservative composition for animal feed, characterized in that 35%-95% alcohol.

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