JP3274677B1 - Eggs with active oxygen scavenging ability and their production method - Google Patents

Abstract

[PROBLEMS] To provide an egg which has essentially no active oxygen scavenging ability and which strongly possesses an active oxygen scavenging action, and to provide an egg and a production method thereof. SOLUTION: A method for producing an active oxygen-eliminating egg, characterized in that a certain amount or more of an iodine compound and / or seaweed is administered to birds so that 4.2 ppm or more of iodine is contained in eggs.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition having an ability to scavenge active oxygen, and more particularly to a composition having an ability to scavenge active oxygen containing iodine-containing bird-derived eggs as an active ingredient.

[0002]

2. Description of the Related Art Conventionally, active oxygen is considered to have harmful toxicity to living tissues, and has been cited as one of the important causes of skin aging, cancer, stroke, rheumatism and various other diseases. Chemical Dictionary 2nd edition, p. 699, 19
February 5, 1991, Tokyo Kagaku Doujin, Iwanami Physical and Chemical Dictionary, 3rd edition, supplemental edition, p. 749, February 20, 1986, U.S. Pat. No. 4,022,224, JP-A-63-79834).

As active oxygen generated in a living body, a superoxide anion radical, a hydrogen peroxide, a hydroxyl radical, a singlet oxygen, and a peroxy radical and an alkoxy radical generated by peroxidation of lipids are known. These active oxygens cause damage to tissues due to cross-linking of collagen fibers, damage to DNA, and generation of chain radicals, resulting in aging of skin and living bodies, inflammation, and smooth muscle damage during myocardial infarction. It is known to cause liver damage, dementia due to destruction of brain tissue, and induction of carcinogenesis.

[0004] Therefore, elimination of active oxygen generated in a living body is considered to be important for prevention or treatment of these diseases. Particularly in the elderly, it is considered that the biological defense ability for protecting the living body from the oxidative stress disorder of DNA is reduced, and thus it is necessary to supplement the antioxidant from outside the body. As a complementary therapy, there is great expectation for the antioxidant action of many antioxidants, including polyphenols contained in food (Longevity Science, p. 223, 2).
(September 29, 2000, Science Forum Inc.). From such a viewpoint, a search for a substance capable of eliminating active oxygen has been widely performed.

[0005] Japanese Patent Publication No. 5-19531 discloses an active oxygen-suppressing composition obtained by adding a plant oil from a roasted plant to a fermented product obtained by adding a microorganism to a plant seed or germ roasted with far infrared rays. Has been proposed. The plants exemplified in this publication are grains such as rice, wheat and barley, and beans such as soybean and corn. Further, JP-A-6-1
No. 28121 discloses water of Asparasas linearis,
It discloses that the organic solvent extract has an active oxygen eliminating action.

[0006] Although various proposals have been made to suppress the action of active oxygen, natural materials and their extracts,
Furthermore, the additives are the main ones. The livestock products were developed by the present applicant (Japanese Patent Application No. 2000-23101).
No. 6) only proposes that livestock products fed with feed containing extruded soybeans have an active oxygen scavenging effect. This indicates that when raw materials having active oxygen scavenging ability were blended, active oxygen scavenging ability was observed in livestock products, but it was obtained by blending raw materials without active oxygen scavenging ability with feed. No mention of livestock products.

[0007] Further, a technique for improving the quality of livestock products by devising feed is disclosed in Japanese Patent Application Laid-Open No. 2000-125781.
JP-A-11-155495, JP-A-10-056
No. 978. However, none of these prior art publications disclose livestock products having an active oxygen-scavenging ability obtained as a result of blending raw materials having no active oxygen-scavenging ability with feed.

[0008]

SUMMARY OF THE INVENTION It is an object of the present invention to provide an egg having essentially no ability to scavenge active oxygen to strongly possess an active oxygen scavenging action.

[0009]

Means for Solving the Problems The present inventors are concerned with eggs which can be said to be essential foods in daily life, the effects of trace components added to feed on egg components, and the effects of egg components on lifestyle-related diseases. During their research, they found that iodine-rich eggs had the ability to scavenge active oxygen, which has harmful effects on the human body.

That is, the present invention provides (1) a composition for eliminating active oxygen containing an egg containing 4.2 ppm or more of iodine as an active ingredient, and (2) a composition for eliminating active oxygen described in (1). (3) A certain amount of iodine compound and / or seaweed is administered to birds in a certain amount or more.
A method for producing an active oxygen-eliminating egg characterized by containing 2 ppm or more of iodine, wherein (4) the iodine compound is at least one of calcium iodate, potassium iodate and potassium iodide. (3) The method according to the above. “Egg containing high iodine”, which is an active ingredient of the composition according to the present invention, is usually obtained as follows.

That is, iodine compounds such as calcium iodate, potassium iodate, potassium iodide, sodium iodate, thymol iodide, copper iodide, hypoiodosalicylic acid, calcium periodate, calcium iodobehemite, and kelp A certain amount of seaweed, such as kelp, containing iodine, or a processed product thereof is mixed with a feed in a certain amount or more, and the mixture is administered to birds to increase the iodine content in eggs. At this time, it is preferable to use any one or more of calcium iodate, potassium iodate or potassium iodide from the viewpoint of bird health, iodine transfer to eggs, and the like, and it is further preferable to use seaweeds such as kelp in combination. .

The amount of iodine to be administered to birds is as follows:
Depending on the type of bird, for example, chicken, quail, etc., in the case of laying hens, about 5-250 mg per bird per day, preferably 5-1
It is better to administer about 5mg. Usually, it is mixed with the feed and administered. In this case, assuming that about 100 g of feed is fed per bird per day, iodine of 50 to 2,500 ppm, preferably 50 to 150 ppm in the feed may be incorporated.

As described above, when a substance containing a large amount of iodine is administered to birds, an egg containing a large amount of the desired iodine is produced after about one week. For example, in the case of a laying hen, when a feed containing about 50 ppm iodine in the feed is administered, an egg containing about 300 μg of iodine can be obtained per egg, and a feed containing about 100 ppm iodine in the feed can be obtained. Is to produce eggs containing about 600-800 μg iodine per egg.

Usually, the required amount of iodine in a laying hen is 0.30 to 0.35 mg / kg of feed (according to NRC), and the actual amount of iodine in a commercial feed is 0.3 to 2.0 mg / kg. The amount of iodine in normal eggs produced from chickens bred on commercial feeds is approximately 6 μg per chicken (American Egg
Board)), at most about 30μg per piece. In addition, such a normal egg hardly has an active oxygen scavenging ability. Compared with the normal egg, the egg obtained as described above has a much higher content of iodine, and thus has an active oxygen scavenging ability.

The thus-obtained egg containing a high content of iodine can be used as it is as the composition of the present invention, and may be subjected to treatments such as heating, drying, concentration, powdering and granulation. No problem, further mixing various excipients or binders, etc. to use in the form of tablets and powders,
It can also be processed into syrups or drinks.

Since the composition of the present invention is extremely excellent in nutrition and has no side effects, it is desirable to take it continuously. And the iodine content per egg is 300 ~ 1,00
If it is adjusted to about 0 μg and eats one or two eggs a day, it can be ingested very easily in daily life.

Incidentally, if the concentration of 300 μg to 1000 μg is expressed,
The amount of eggs with shells that are mainly distributed in the market is 58 g to 76 g, and the edible portion alone is 50 g to 72 g, so that the amount is 4.2 ppm to 20.0 ppm. 4.2pp
m or less, it is not effective even if it has active oxygen scavenging ability.
Feed efficiency decreases. In the present invention, birds include chickens, quail, crow bone chickens, and the like. In the present invention, "composition"
The egg may be the egg itself or a mixture of the egg and another substance.

[0018]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited thereto.

[0019]

[Example] Calcium iodate was added to a commercially available adult chicken feed so that the amount of iodine became 100 ppm, and the feed was fed to 100 laying hens and reared. As a means of quantifying this active oxygen, a weak luminescence method has been researched and developed. The quantification of lipid peroxide and superoxide using a luminescent reagent (luminol / lucigenin) and the measurement of singlet oxygen by the weak luminescence measurement at 1,280 nm Detection and the like are generally known. In recent years, Okubo et al. Have shown that natural radical scavengers such as flavonoids emit weak light in the presence of hydrogen peroxide and acetaldehyde. Since these weak luminescence are generated in the presence of three kinds of active oxygen species (X), electron donor (Y), and acceptor species (Z), the weak luminescence is used as XYZ-based weak luminescence to produce foods having a function of eliminating active oxygen. Simple search enabled.

That is, a component that generates active oxygen in a living body was defined as an X component, and an antioxidant component that eliminates natural radicals (such as active oxygen) was defined as a Y component. The elimination of active oxygen is not always eliminated if an antioxidant component (Y component) is present, but the present inventors have discovered the presence of a component that catalytically enhances the elimination reaction. The component is a Z component. (FIG. 1) The luminance of this XYZ-based weak light emission was used to measure the active oxygen scavenging ability. The test examples are described below.

(Test Example 1) First, a 12-hole plate (3
(4 rows), place the 4th hole in the first row as a blank (brightness control section). From the second column, 1 ml of the undiluted solution of “egg containing high iodine” and homogenized without separating the albumen and yolk as the liquid volume is 1 ml. Injection. 1 m each of butyl alcohol, 2% hydrogen peroxide (X reagent), and saturated gallic acid solution (Y reagent)
l Added to all holes. This was put into a measurement room equipped with a VIM camera manufactured by Hamamatsu Photonics, and the weak luminescence generated by the reaction was photographed 10 minutes later. The ability was evaluated. The results, as shown in Table 1, revealed that the test group had a remarkable predisposition as the recipient species (Z) as compared to the control group. In other words, this indicates that “egg containing high iodine” has active oxygen scavenging ability.

[0022]

[Table 1]

(Test Example 2) 96-well plate (8 × 12 rows)
Place 8 holes in the first row as blanks (brightness control).
Those homogenized from the second column to "eggs having a high content of iodine" egg breaking and without dividing the albumen, yolk 2-fold dilutions (× 2 ⁰
~ 2 ⁹⁾ samples and similarly the ordinary egg solution (control group) 0.5 ml injection of the liquid volume from further for one column.
Further, 0.5 ml of butyl alcohol and 2% hydrogen peroxide (X reagent) were added to all the wells. This was put into a measurement room equipped with a VIM camera manufactured by Hamamatsu Photonics, and the faint luminescence generated by the reaction was photographed after 10 minutes. Table 2 shows the results indicating the degree. It is clear that the test group, that is, the “egg containing high iodine” has a higher active oxygen scavenging ability than the control group.

[0024]

[Table 2]

(Test Example 3) In the same manner, a test was conducted to compare the active oxygen scavenging ability of the feed of ordinary eggs and the feed of "eggs containing high iodine". On a 12-well plate (3 x 4 rows)
The applicant of the present invention has proposed that the first row is a blank, and the common egg feed and the "egg containing high iodine" feed are positive control plots in the last row, three holes per row from the second row. Feed containing extruded soybeans described in Japanese Patent Application No. 2000-231016 (corn 50, milo 5, barley 1)
0, soybean meal 10, extruded soybean 15, molasses 2, rice bran 5, salt 0.3, calcium carbonate 1.2, calcium phosphate
1.1 and vitamin / mineral mixture 0.4) were each put in a volume of 1 ml. Here, 2 ml of butyl alcohol and 2% hydrogen peroxide (X reagent) were respectively added to all the wells. Table 3 shows the results.

That is, in the ordinary egg feed and the feed of "egg containing high iodine", no faint luminescence indicating active oxygen scavenging ability was observed. This indicates that "egg containing high iodine" has active oxygen scavenging ability as in Test Examples 1 and 2, even if the feed does not have active oxygen scavenging ability. .

[0027]

[Table 3]

[0028]

According to the present invention, a raw material having no active oxygen scavenging ability can be used to retain the active oxygen scavenging ability, thereby preventing the adverse effects of active oxygen.

[Brief description of the drawings]

FIG. 1 is a view showing a mechanism of “XYZ-based weak light emission”.

────────────────────────────────────────────────── ─── Continued on the front page (51) Int.Cl. ⁷ Identification code FI A23L 1/32 A23L 1/32 Z A61K 33/18 A61K 33/18 A61P 9/00 A61P 9/00 17/00 17/00 29 / 00 101 29/00 101 35/00 35/00 39/06 39/06 43/00 105 43/00 105 111 111 (56) References JP-A-59-116225 (JP, A) JP-A-4- 99728 (JP, A) JP-A-53-24065 (JP, A) JP-A-56-138119 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 35/54 A23K 1 / 175 A23L 1/32 JICST file (JOIS)

Claims (2)

(57) [Claims] 1. A composition for eliminating active oxygen (except for wound healing, anti-inflammation, anti-aging, and anti-cancer), comprising an egg derived from a bird containing 4.2 ppm or more of iodine as an active ingredient. 2. An active oxygen scavenging composition (excluding wound healing, anti-inflammation, anti-aging, and anti-cancer compositions) according to claim 1, which is used for eliminating active oxygen. Syrup or beverage except for inflammation, anti-aging, anti-cancer).