KR102192712B1 - An animal feed composition comprising phlorotannin and product for animal - Google Patents

Abstract

The present invention relates to a composition for animal feed that has antioxidant and anti-inflammatory properties containing fluorotannin extracted from brown algae, and can be applied safely on a daily basis, especially using natural ingredients, and the gloss, smell, stool smell, discoloration of hair, It relates to a composition and product having the function of improving overweight.

Description

An animal feed composition comprising phlorotannin and product for animal}

The present invention relates to a composition for animal feed and a product for animals comprising as an active ingredient fluorotannin having antioxidant and inflammation-improving effects. The composition and product for animals of the present invention give gloss to the animal's hair through antioxidant effect, inflammation improvement effect, etc., improve animal odor, stool odor, discoloration, obesity, and have a function of contributing to psychological stability. In addition, the present invention relates to a composition for animal feed and a product for animals that can be safely taken for a long time using natural ingredients as a main ingredient.

As the number of single-person households increases and the average income increases, the number of households raising companion animals is increasing. As the number of companion animals increases, the scale of related industries is also increasing.According to the status of the companion animal feed industry of the Korea Rural Economic Institute, the size of the feed industry increased from 250 billion won in 2012 to 460 billion won in 2016. In addition, the average life expectancy of companion animals is also increasing through the increase of vaccinations through the improvement of awareness of the companion animal family and improvement of the quality of food.

However, problems due to overweight or nutritional imbalance are occurring as companion animals consume excessive feed and snacks and eat human foods. The weight gain in animals irritates the joints, and continuous irritation can cause inflammatory diseases and degenerative arthritis. Occurrence of arthritis and inflammatory diseases may cause psychological anxiety during the life of the companion animal and adversely affect the companion animal's emotions. Accordingly, among companion animal owners, interest in degenerative diseases caused by aging such as arthritis and dementia of companion animals is increasing, and interest in daily health management is also increasing.

Inflammation is caused by pathogens, irritants, etc., and functions such as inhibition of early cell damage and tissue regeneration, but when the inflammation becomes severe, it may cause diseases such as inflammatory diseases. In order to prevent inflammation from developing into a disease, immunity needs to be strengthened, which means that normal nutrient intake and functional food are required. In addition, in the case of animals, unlike humans, it is impossible to express intentions, so it is necessary to administer functional foods with antioxidant and anti-inflammatory functions to feed and snacks that are normally consumed.

Looking at the existing prior art for animal feed, according to Korean Patent Registration No. 10-1777842, animal feed with kelp is provided, and Korean Patent Application Publication No. 10-2005-0099436 provides anti-allergic and anti-inflammatory activity. Provide containing animal feed additives.

In the present invention, unlike the existing patents, it is intended to provide a composition for animal feed containing fluorotannin derived from brown algae and having antioxidant and anti-inflammatory functions, and to provide an animal product including the functional raw material.

(Public Patent 1) Korean Patent Registration No. 10-1777842 (Public Patent 2) Korean Patent Publication No. 10-2005-0099436

The present invention provides a composition for animal feed and a product for animals containing fluorotannin having antioxidant and anti-inflammatory functions as an active ingredient, thereby helping the daily health management of animals and reducing the occurrence of inflammatory diseases, Try to improve liver satisfaction. In addition, it is intended to provide a variety of products such as powdered products, animal feed, and snacks so that users can conveniently access and apply them.

In order to achieve the above object, the present invention provides a composition for animal feed comprising fluorotannin as an active ingredient.

In addition, the present invention provides a product for animals comprising the composition.

In addition, the present invention provides a method of using a composition containing fluorotannin as an active ingredient as animal feed.

The composition for animal feed containing fluorotannin of the present invention as an active ingredient contains natural materials and does not contain preservatives or chemicals, so it can be used safely and there is no fear of side effects, and can be applied in various forms such as feed and snacks. This is possible. In addition, the product of the present invention is applied to products that are used on a daily basis, helping to manage the animal's usual health and enhancing immunity.

1 is a diagram showing Product Example 1 of Example 2.
2 is a diagram showing the packaging state of Product Example 2 of Example 3.
3 is a diagram showing Product Example 2 of Example 3. FIG.
4 is a diagram showing the antioxidant effect of the composition of Example 1.
5 is a diagram showing the NO production inhibitory effect of the composition of Example 1.
6 is a diagram showing the PGE2 production inhibitory effect of the composition of Example 1.
7 is a diagram showing the COX-2 production inhibitory effect of the composition of Example 1.

Hereinafter, the present invention will be described in detail.

As the number of single-person households increases, the number of households raising companion animals is increasing, and the survival age is prolonged due to the development of feed and medicines for companion animals, which raises the issue of health management of elderly companion animals. Degenerative and inflammatory diseases such as arthritis, stomatitis, and dementia that occur in older companion animals can cause lifelong discomfort and adversely affect the emotions of people who keep companion animals. In addition, obesity and nutritional imbalance caused by irregular nutritional supply of companion animals can worsen the health of animals.

Therefore, in the present invention, the present invention was completed by providing a composition for animal feed with anti-inflammatory and antioxidant activity, and developing a product using the composition.

The present invention provides a composition for animal feed comprising a fluorotannin compound as an active ingredient.

The composition for animal feed containing fluorotannin of the present invention as an active ingredient improves the health of animals by performing antioxidant and anti-inflammatory functions.

The present invention provides a method of using a composition containing fluorotannin as an active ingredient as animal feed. When the composition is used as a feed, the composition improves the health of animals by having an antioxidant and anti-inflammatory function.

The composition for animal feed may contain 20 to 100% by weight of a fluorotannin compound.

The composition for animal feed is a natural ingredient and can be obtained by any conventional method. That is, it may be synthesized using commercially available reagents, and may be obtained by extraction and separation from natural products, especially seaweed.

The composition for animal feed may be an algae extract. The composition for animal feed may be an alcohol extract of seaweed.

The composition for animal feed using fluorotannin as an active ingredient can be extracted from algae, particularly brown algae, and specifically, Rhubarb (Eisenia bicyclis), Eisenia arborea, Eisenia desmarestioides ), Eisenia galapagensis, Eisenia masonii, Ecklonia kurome, Ecklonia cava, Ecklonia stolonifera, Ecklonia maxima, Ecklonia maxima Ecklonia radiata, Ecklonia bicyclis, Ecklonia biruncinate, Ecklonia buccinalis, Ecklonia caepaestipes, Ecklonia caepaestipes Ecklonia exasperta, Ecklonia fastigiata, Ecklonia brevipes, Ecklonia arborea, Ecklonia latifolia, Eclonia It is extracted from one or more seaweeds selected from the group consisting of Ecklonia muratii, Ecklonia radicosa, Ecklonia richardiana and Ecklonia wrightii.

In addition to fluorotannin, the composition of the present invention may additionally contain 0-80% by weight of components consisting of mannitol, alginic acid, fucoidan, laminaran as a carbohydrate component, and fucosterol, fucoxanthine as a lipid component.

The composition for animal feed of the present invention may include one or more fluorotannins selected from compounds represented by the following Chemical Formulas 1 to 12.

[Formula 1]

[Formula 2]

[Formula 3]

[Formula 4]

[Formula 5]

[Formula 6]

[Formula 7]

[Formula 8]

[Formula 9]

[Formula 10]

[Formula 11]

[Formula 12]

The composition for animal feed of the present invention may contain one or more fluorotannins selected from the group consisting of Chemical Formula 2, Chemical Formula 4, Chemical Formula 5, and Chemical Formula 8 as an active ingredient. When the fluorotannin is included in an appropriate amount, it is excellent in antioxidant and inflammation improvement effects.

The composition for animal feed of the present invention is preferably 1 to 60% by weight of fluorotannin of formula 2, 0.3 to 20% by weight of fluorotannin of formula 4, 0.1 to 10% by weight of fluorotannin of formula 5 or It may contain 0.1 to 5% by weight of the fluorotannin of Formula 8.

The composition for animal feed of the present invention comprises 0.1 to 3% by weight of fluorotannin of formula 1, 5 to 63% by weight of fluorotannin of formula 2, 1 to 3% by weight of fluorotannin of formula 3, and 0.5 to 34% by weight of fluorotannin, 0.1 to 50% by weight of fluorotannin of formula 5, 0.5 to 8% by weight of fluorotannin of formula 6, 0.1 to 1% by weight of fluorotannin of formula 7 0.1 to 46 wt% tannin, 0.1 to 2 wt% fluorotannin of formula 9, 0.1 to 7 wt% fluorotannin of formula 10, 0.1 to 3 wt% fluorotannin of formula 11 and 0.1 fluorotannin of formula 12 It may contain ~ 4% by weight.

The composition of the present invention comprises 20 to 100% by weight of fluorotannin, 0 to 30% by weight of mannitol, 0 to 25% by weight of alginic acid, 0 to 25% by weight of fucoidan, 0 to 10% by weight of fucosterol, 0 to 10% by weight of fucoxanthine , Lamina may contain 0 to 10% by weight.

The composition of the present invention contains 20 to less than 100% by weight of fluorotannin, more than 0 to 30% by weight of mannitol, more than 0 to 25% by weight of alginic acid, more than 0 to 25% by weight of fucoidan, more than 0 to 10% by weight of fucosterol, fucoxanthine More than 0 to 10% by weight, laminaran may include more than 0 to 10% by weight.

The composition for animal feed of the present invention may be in the form of a powder or a liquid to facilitate addition to products such as animal feed and animal snacks.

The present invention provides an animal product comprising a composition for animal feed containing the fluorotannin.

Animal products of the present invention include various adjuvants such as amino acids, inorganic salts, vitamins, antibiotics, antibacterial substances, antioxidants, anti-fungal agents, enzymes, living microorganism preparations, etc. as auxiliary ingredients along with a composition for animal feed containing fluorotannin; Grains such as crushed or crushed wheat, oats, barley, corn and rice; Vegetable proteins such as rapeseed, soybeans and sunflowers; Animal proteins such as blood meal, meat meal, bone meal and fish meal; Sugar and dairy products such as various milk powders and whey powder may be included.

The animal product may contain preferably 0.1 to 50% by weight and more preferably 0.2 to 10% by weight of an animal feed composition including fluorotannin based on the total weight of the product, but the blending amount is not limited to the above range. When the animal product of the present invention contains the composition for animal feed in the above amount, it can give the animal's antioxidant effect and anti-inflammatory effect, help health management, and improve immunity.

The present invention, along with the animal feed composition, collagen, flaxseed extract, calcium, iron, vitamin B12, vitamin E, such as vitamins, copper, chicken, pig gelatin, rice flour, soy flour, flour, minerals, chicken oil, sorbitol, propylene glycol , Glycerin, fish and water to provide an animal product containing at least one selected from the group

The product is preferably 1 to 50% by weight of the composition for animal feed of the present invention and 40 to 80% by weight of collagen, 1 to 4% by weight of flaxseed extract, 5 to 15% by weight of calcium, 0.1 to 0.8% by weight of iron, and 0.008 of vitamin B12 It may contain ~ 0.01% by weight, 0.1 ~ 0.3% by weight of vitamin E, and 0.05 ~ 0.1% by weight of copper. In this case, there is an effect of reducing the occurrence of inflammatory diseases in animals and improving animal health.

The animal product of the present invention may be commercialized as a powder or granular animal feed, or snacks such as dog gum and canned food.

The animal product of the present invention may contain 80 to 97% by weight of at least one selected from the group consisting of chicken, pig gelatin, rice flour, soy flour, and wheat flour. In this case, there is an advantage in that it is easy to commercialize while providing a balanced nutritional diet to animals.

The daily amount of fluorotannin contained in the animal product of the present invention is preferably 0.1 to 100 mg, more preferably about 1 to 50 mg, but the amount used is not limited to the above range.

Hereinafter, the present invention will be described in more detail through examples and experimental examples. However, the following examples and experimental examples are intended to more specifically illustrate the present invention, and the scope of the present invention is not limited by the following examples and experimental examples. The following Examples and Experimental Examples can be appropriately modified and changed by those skilled in the art within the scope of the present invention.

Example 1. Preparation of a composition containing fluorotannin

In order to prepare a composition for animal feed containing fluorotannin of the present invention as an active ingredient, it was extracted from domestic brown algae by the following method.

Ecklonia cava was washed with distilled water to remove foreign substances, dried in the shade, and then crushed. 500g of the Ecklonia cava was put in an extraction container, and a 30% by weight aqueous alcohol solution of 20 times the weight of Ecklonia was added, followed by reflux extraction at 75 to 80°C for 4 hours to obtain an extract. The extract was filtered through a 10 μm filter network, and the filtered extract was concentrated under reduced pressure to obtain a first extract concentrate. The primary extract concentrate was dried with a vacuum dryer and then pulverized to obtain 170 g of Composition 1.

The composition 1 was dissolved in hot water at 50° C., adsorbed on an adsorption resin, washed with water, and eluted with alcohol to obtain a fluorotannin eluate. The eluate was filtered through a 1 μm filter network, and the filtered extract was concentrated under reduced pressure to obtain a second extract concentrate. The second extract concentrate was dried with a vacuum dryer and then pulverized to obtain 30 g of Composition 2.

Thereafter, the composition 2 was suspended in 20 times the amount of distilled water, and extracted three times with the same amount of ethyl acetate solvent to obtain an ethyl acetate fraction. The ethyl acetate fraction was concentrated under reduced pressure, and the concentrated solution was dried with a vacuum dryer and then pulverized to obtain 10 g of composition 3

Compound separation was carried out by loading the compositions 1 to 3 prepared above in liquid chromatography. At this time, it was confirmed that the obtained compound is a compound of Formulas 1 to 12 using a compound structure analysis device NMR. The composition prepared above was dissolved in 60% by volume of methanol, filtered through a 0.2 μm membrane filter, and loaded on high performance liquid chromatography. In high performance liquid chromatography, HP ODS Hypersil was used as the column and distilled water and methanol were used as the solvent, and the supply of the solvent was a linear gradient from 15% by volume to 70% by volume of methanol at a flow rate of 1.0 ml/min over 30 minutes. ) As a result of analysis, it was confirmed the presence of the compounds of Formulas 1 to 12. The chemical composition of the composition is shown in Table 1 below.

sample Example 1 Composition 1 Formula 2 2% by weight, Chemical formula 4 1% by weight, Chemical formula 5 0.5% by weight, Chemical formula 8 0.3% by weight, 20% by weight of total fluorotannin containing 16.2% by weight of other single substances of the formula 1-12, mannitol 25% by weight %, alginic acid 20% by weight, fucoidan 20% by weight, fucosterol 5% by weight, fucoxanthine 5% by weight, laminaran 5% by weight Composition 2 Formula 2 10% by weight, Chemical formula 4 3% by weight, Chemical formula 5 1% by weight, Chemical formula 8 1.5% by weight, 70% by weight of total fluorotannin including 54.5% by weight of a single substance of formula 1-12, mannitol 10% by weight , Alginic acid 6% by weight, Fucoidan 6% by weight, Fucosterol 3% by weight, Fucoxanthine 3% by weight, Laminaran 2% by weight Composition 3 Chemical formula 2 17% by weight, Chemical formula 4 5% by weight, Chemical formula 5 5% by weight, Chemical formula 8 3% by weight, 100% by weight of total fluorotannin including 70% by weight of other single substances of formula 1-12

Example 2. Preparation of powdered product example 1 using the composition

In order to prepare a powdered product using the composition of Example 1, Collagen, flaxseed extract, calcium, iron, vitamin B12, vitamin E, and copper were added to Composition 2 to prepare Product Example 1. The composition of Product Example 1 is shown in [Table 2] below. The manufactured Product Example 1 is shown in [Fig. 1].

Ingredient name content(%) Composition 2 5 Colaken 75 Flaxseed extract 4 calcium 15 iron content 0.7 Vitamin B12 0.01 Vitamin E 0.2 Copper 0.09 Sum 100

Product Example 1 itself can be fed to animals, and can be mixed with general feed. In addition, it can be easily used by mixing with snacks such as canned food and chicken breast.

Example 3. Preparation of Product Example 2

Animal feed was prepared by adding other raw materials to Product Example 1 (Product Example 2), and the composition is shown in [Table 3] below. Product Example 2 can be fed as a general feed, and the feeding amount is 1.5 to 3 g per time for animals weighing less than 15 kg, 2.5 to 5 g for animals weighing less than 15 to 30 kg, and 5 to 7.5 g for animals weighing 30 kg or more. The prepared animal feed is shown in [Fig. 2] and [Fig. 3].

Ingredient name content(%) Product Example 1 8 Hydrolyzed Chicken 6 Pig Gelatin 5 Rice flour 33 Soybean flour 15 flour 4 Vitamins and minerals 0.1 Chicken Oil 3 Sorbitol, propylene glycol 17.3 glycerin 2.6 fish 2 water 4 Sum 100

Experimental Example 1. Antioxidant effect evaluation experiment of composition

The antioxidant effect of the composition of Example 1 was evaluated as follows.

After dissolving the composition of Example 1 and vitamin C known to have antioxidant effects at a concentration of 1 mg/mL in 50% ethanol aqueous solution, respectively, in a 10% ratio in a DPPH (1,1-diphenyl-2-picrylhydrazyl) solution having an absorbance of 0.94 to 0.98. After adding, let stand for 10 minutes. After 10 minutes, the absorbance is measured to calculate the radical scavenging rate. The method of calculating the radical scavenging rate is as follows.

Radical scavenging rate (%) = (Initial absorbance-absorbance after 10 minutes) / Initial absorbance X 100

The results of Experimental Example 1 are shown in [Fig. 4]. As shown in Fig. 4, the composition of the present invention has an antioxidant activity equivalent to that of vitamin C.

Experimental Example 2. Evaluation of the anti-inflammatory effect of the composition

In order to confirm the anti-inflammatory effect of the composition on animals, the anti-inflammatory effect was evaluated by measuring the concentration of NO, PGE2, and COX-2 after LPS (lipopolysaccharide) was treated in a mouse macrophage cell line (RAW 264.7).

To measure the NO concentration, the composition of Example 1 was pretreated to culture cells at different concentrations (30 to 120 μg/mL), and then LPS was treated after 1 hour to induce an inflammatory response. After 24 hours of LPS treatment, the culture solution and the same amount of grease reagent were mixed and absorbance was measured at 530 nm. To calculate the NO concentration, a standard curve was drawn up using the absorbance of each concentration of NaNO ₂ and the concentration was calculated.

The concentrations of PGE2 and COX-2 were measured using an enzyme-linked immunosorbent assay (ELISA assay). After the cell culture solution was treated with LPS and the composition, when one day elapsed, the absorbance was measured at 540 nm after inoculation into the enzyme-linked immunosorbent kit. The concentration of each protein was calculated after preparing a standard curve with 4-PL logistic curve fit using the absorbance results obtained by treating the standard product by concentration. The anti-inflammatory effect evaluation results are shown in [Fig. 5] to [Fig. 7]. Significance of results was confirmed by using a T-test were P <based on 0.05 (* P <0.05 vs control , # P <0.05 vs LPS).

As shown in the experimental results of [Fig. 5] to [Fig. 7], it can be seen that the composition of the present invention significantly alleviates the inflammatory reaction caused by LPS.

According to [Fig. 5], it can be seen that the concentration of NO decreases when the composition of the present invention is treated with LPS in the experiment using mouse macrophages, and the concentration of NO decreases further as the concentration increases. have. This means that the composition of the present invention reduces inflammation by suppressing the production of NO, an inflammatory reaction product caused by LPS.

According to Fig. 6, in the experiment using mouse macrophages, when the composition of the present invention was treated with LPS, it was confirmed that the concentration of COX-2 decreased, and the concentration of COX-2 decreased further as the concentration increased. I can see that. This means that the composition of the present invention reduces inflammation by inhibiting the production of COX-2, an inflammatory protein caused by LPS.

According to [Fig. 7], it can be seen that the concentration of PGE2 decreases when the composition of the present invention is treated with LPS in the experiment using mouse macrophages, and the concentration of PGE2 decreases further as the concentration increases. have. This means that the composition of the present invention reduces inflammation by inhibiting the production of PGE2, an inflammatory reaction protein caused by LPS.

Experimental Example 3. Efficacy evaluation experiment of Product Examples 1 and 2

A sample prepared in the form of a capsule by taking 0.1 g of the powder of Product Example 1 and the sample of Product Example 2 were continuously fed with feed to the animals to be tested for 1 month. The efficacy of the product examples was evaluated by evaluating the odor, discoloration, stool odor, and gloss of the hair of the animals before and after feeding. The evaluation method was evaluated as 2 (bad), 4, 6, 8, 10 (good) using a 5-point scale. For the objective experiment results, only animals with no abnormality in health were selected for the animals to be tested, and the species of animals to be tested was dogs. Depending on the weight of the experimental animals, Product Example 1 was fed twice a day by mixing 1 capsule for animals weighing less than 15 kg, 2 capsules for animals weighing 15 to 30 kg, and 3 capsules for animals weighing 30 kg or more. In Product Example 2, 1.5 g of animals under 15 kg, 2.5 g of animals under 15 to 30 kg, and 5 g of animals over 30 kg were fed twice a day with feed. The evaluation of the animals fed Product Examples 1 and 2 is shown in Table 4 below.

As shown in the experimental results in the table, it was confirmed that the gloss, odor, stool odor, and discoloration of the fur of the fed animals were improved in Product Examples 1 and 2 of the present invention.

division Efficacy evaluation (the higher the score, the better) Gloss of hair smell Smell Discoloration Before salary After salary Before salary After salary Before salary After salary Before salary After salary Product Example 1 5.6 8 5.6 8.8 4.6 7.8 5.6 7.4 Product Example 2 5.4 8 5.6 8.6 4.2 6.6 5.6 7.2

Experimental Example 4. Obesity improvement efficacy evaluation experiment of Product Example 2

In order to evaluate the effect of improving the obesity of the animal feed of Product Example 2, 10 dogs that were 20% overweight than the normal weight were selected and fed with Product Example 2 when feeding the feed. Product Example 2 was fed 1.5 g for animals less than 15 kg, 2.5 g for animals less than 15 to 30 kg, and 5 g for animals weighing 30 kg or more twice a day. I did. For the objective experiment results, the animals that had grown up were selected as the animals to be tested, and animals with no abnormalities other than obesity were selected. The animal's overweight rate was calculated as follows using the standard weight for each breed.

Overweight rate (%) = (current weight-normal weight)/normal weight

The results of the evaluation of the obesity improvement efficacy of Product Example 2 are shown in Table 5 below.

As shown in the experimental results in the table, it was confirmed that obesity in dogs fed Product Example 2 of the present invention was improved.

division Overweight rate before salary (%) Overweight rate after salary (%) Product Example 2 20.69 8.17

Claims (13)

delete delete A composition for animal feed containing 20-100% by weight of the total fluorotannin compound, 1 to 60% by weight of fluorotannin of the following formula (2), 0.3 to 20% by weight of fluorotannin of formula 4, and 0.1 of fluorotannin of formula (5) Companion animal feed composition comprising 0.1 to 5% by weight to 10% by weight and fluorotannin of Formula 8
[Formula 2]

[Formula 4]

[Formula 5]

[Formula 8]

delete The method of claim 3, wherein the composition further comprises one or more polotannins selected from the group consisting of the following Formula 1, Formula 3, Formula 6, Formula 7, Formula 9, Formula 10, Formula 11, and Formula 12 Companion animal feed composition, characterized in that
[Formula 1]

[Formula 3]

[Formula 6]

[Formula 7]

[Formula 9]

[Formula 10]

[Formula 11]

[Formula 12]

The composition for companion animal feed according to claim 3, wherein the composition for companion animal feed is an algae extract. The method according to claim 3, wherein the fluorotannin compound is Eisenia bicyclis, Eisenia arborea, Eisenia desmarestioides, Eisenia galapagensis, Eisenia masonii, Ecklonia kurome, Ecklonia cava, Ecklonia stolonifera, Ecklonia maxima, Ecklonia radiata, Ecklonia bicyclis Ecklonia bicyclis), Ecklonia biruncinate, Ecklonia buccinalis, Ecklonia caepaestipes, Ecklonia exasperta, Ecklonia pasti Ecklonia fastigiata, Ecklonia brevipes, Ecklonia arborea, Ecklonia latifolia, Ecklonia muratii, Ecklonia radicosa radicosa), Ecklonia richardiana (Ecklonia richardiana) and Ecklonia wrightii (Ecklonia wrightii). The method according to claim 3, 20 to 100% by weight of fluorotannin, 0 to 30% by weight of mannitol, 0 to 25% by weight of alginic acid, 0 to 25% by weight of fucoidan, 0 to 10% by weight of fucosterol, 0 to 10% by weight of fucoxanthine And a composition for companion animal feed comprising 0 to 10% by weight of laminaran Companion animal product comprising the composition of claim 3 The product for companion animals according to claim 9, wherein the product is in the form of animal feed, canned food for animals, or dog gum. The product of claim 9, wherein the product comprises 0.1 to 50% by weight of the composition based on the total weight of the product. The companion animal product of claim 9, wherein the product further comprises at least one selected from the group consisting of collagen, flaxseed extract, calcium, iron, vitamin B12, vitamin E, and copper. The method of claim 12, wherein the product is the composition 1 to 50% by weight, collagen 40 to 80% by weight, flaxseed extract 1 to 6% by weight, calcium 0.001 to 18% by weight, iron content 0.1 to 1% by weight, vitamin B12 0.00001 to 0.01 Companion animal product containing 0.1 to 0.5% by weight of vitamin E and 0.05 to 0.1% by weight of copper

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