CN111669976A - Composition for animal feed containing brown algae polyphenol as active ingredient and animal product - Google Patents
Composition for animal feed containing brown algae polyphenol as active ingredient and animal product Download PDFInfo
- Publication number
- CN111669976A CN111669976A CN201980009505.5A CN201980009505A CN111669976A CN 111669976 A CN111669976 A CN 111669976A CN 201980009505 A CN201980009505 A CN 201980009505A CN 111669976 A CN111669976 A CN 111669976A
- Authority
- CN
- China
- Prior art keywords
- composition
- chemical formula
- brown algae
- animal feed
- animal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001465754 Metazoa Species 0.000 title claims abstract description 127
- 239000000203 mixture Substances 0.000 title claims abstract description 93
- 241000199919 Phaeophyceae Species 0.000 title claims abstract description 56
- 235000013824 polyphenols Nutrition 0.000 title claims description 51
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims description 45
- 239000004480 active ingredient Substances 0.000 title claims description 13
- 229920000855 Fucoidan Polymers 0.000 claims abstract description 10
- 239000000126 substance Substances 0.000 claims description 61
- 241001512723 Ecklonia Species 0.000 claims description 17
- 239000000284 extract Substances 0.000 claims description 15
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 12
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 12
- 241001474374 Blennius Species 0.000 claims description 7
- 239000010949 copper Substances 0.000 claims description 7
- 102000008186 Collagen Human genes 0.000 claims description 6
- 108010035532 Collagen Proteins 0.000 claims description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 6
- 241001512722 Ecklonia cava Species 0.000 claims description 6
- 241000230129 Eisenia <Phaeophyceae> Species 0.000 claims description 6
- 229930003779 Vitamin B12 Natural products 0.000 claims description 6
- 229930003427 Vitamin E Natural products 0.000 claims description 6
- 229910052802 copper Inorganic materials 0.000 claims description 6
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 6
- 229910052742 iron Inorganic materials 0.000 claims description 6
- -1 polyphenol compound Chemical class 0.000 claims description 6
- 235000019163 vitamin B12 Nutrition 0.000 claims description 6
- 239000011715 vitamin B12 Substances 0.000 claims description 6
- 235000019165 vitamin E Nutrition 0.000 claims description 6
- 239000011709 vitamin E Substances 0.000 claims description 6
- 229940046009 vitamin E Drugs 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- 239000011575 calcium Substances 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 229920001436 collagen Polymers 0.000 claims description 5
- 235000004426 flaxseed Nutrition 0.000 claims description 5
- OSELKOCHBMDKEJ-UHFFFAOYSA-N (10R)-3c-Hydroxy-10r.13c-dimethyl-17c-((R)-1-methyl-4-isopropyl-hexen-(4c)-yl)-(8cH.9tH.14tH)-Delta5-tetradecahydro-1H-cyclopenta[a]phenanthren Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)CCC(=CC)C(C)C)C1(C)CC2 OSELKOCHBMDKEJ-UHFFFAOYSA-N 0.000 claims description 4
- CQSRUKJFZKVYCY-UHFFFAOYSA-N 5alpha-isofucostan-3beta-ol Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(C)CCC(=CC)C(C)C)C1(C)CC2 CQSRUKJFZKVYCY-UHFFFAOYSA-N 0.000 claims description 4
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 4
- GBBBJSKVBYJMBG-QTWVXCTBSA-N Fucosterol Natural products CC=C(CC[C@@H](C)[C@@H]1CC[C@@H]2[C@H]3C=C[C@@H]4C[C@H](O)CC[C@@]4(C)[C@@H]3CC[C@@]12C)C(C)C GBBBJSKVBYJMBG-QTWVXCTBSA-N 0.000 claims description 4
- OSELKOCHBMDKEJ-VRUYXKNBSA-N Isofucosterol Natural products CC=C(CC[C@@H](C)[C@H]1CC[C@@H]2[C@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C)C(C)C OSELKOCHBMDKEJ-VRUYXKNBSA-N 0.000 claims description 4
- 229920001543 Laminarin Polymers 0.000 claims description 4
- 229930195725 Mannitol Natural products 0.000 claims description 4
- 229920002230 Pectic acid Polymers 0.000 claims description 4
- OSELKOCHBMDKEJ-JUGJNGJRSA-N fucosterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CC\C(=C/C)C(C)C)[C@@]1(C)CC2 OSELKOCHBMDKEJ-JUGJNGJRSA-N 0.000 claims description 4
- SJWWTRQNNRNTPU-ABBNZJFMSA-N fucoxanthin Chemical compound C[C@@]1(O)C[C@@H](OC(=O)C)CC(C)(C)C1=C=C\C(C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)C(=O)C[C@]1(C(C[C@H](O)C2)(C)C)[C@]2(C)O1 SJWWTRQNNRNTPU-ABBNZJFMSA-N 0.000 claims description 4
- AQLRNQCFQNNMJA-UHFFFAOYSA-N fucoxanthin Natural products CC(=O)OC1CC(C)(C)C(=C=CC(=CC=CC(=CC=CC=C(/C)C=CC=C(/C)C(=O)CC23OC2(C)CC(O)CC3(C)C)C)CO)C(C)(O)C1 AQLRNQCFQNNMJA-UHFFFAOYSA-N 0.000 claims description 4
- DBTMGCOVALSLOR-VPNXCSTESA-N laminarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)OC1O[C@@H]1[C@@H](O)C(O[C@H]2[C@@H]([C@@H](CO)OC(O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-VPNXCSTESA-N 0.000 claims description 4
- 239000000594 mannitol Substances 0.000 claims description 4
- 235000010355 mannitol Nutrition 0.000 claims description 4
- LCLHHZYHLXDRQG-ZNKJPWOQSA-N pectic acid Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)O[C@H](C(O)=O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](OC2[C@@H]([C@@H](O)[C@@H](O)[C@H](O2)C(O)=O)O)[C@@H](C(O)=O)O1 LCLHHZYHLXDRQG-ZNKJPWOQSA-N 0.000 claims description 4
- 239000010318 polygalacturonic acid Substances 0.000 claims description 4
- 241000195493 Cryptophyta Species 0.000 claims description 2
- 241000983748 Ecklonia radiata Species 0.000 claims description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 2
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims 2
- 241000741680 Ecklonia arborea Species 0.000 claims 1
- 241001261727 Exarata Species 0.000 claims 1
- 241000206755 Palmaria Species 0.000 claims 1
- 241000222385 Phanerochaete Species 0.000 claims 1
- 229960005069 calcium Drugs 0.000 claims 1
- 229960005188 collagen Drugs 0.000 claims 1
- 229940107657 flaxseed extract Drugs 0.000 claims 1
- 235000020704 flaxseed extract Nutrition 0.000 claims 1
- 229960003284 iron Drugs 0.000 claims 1
- 230000003078 antioxidant effect Effects 0.000 abstract description 15
- 230000003110 anti-inflammatory effect Effects 0.000 abstract description 13
- 239000003963 antioxidant agent Substances 0.000 abstract description 8
- 210000003608 fece Anatomy 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 239000002932 luster Substances 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 56
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 16
- 239000002158 endotoxin Substances 0.000 description 13
- 229920006008 lipopolysaccharide Polymers 0.000 description 13
- 206010061218 Inflammation Diseases 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 230000004054 inflammatory process Effects 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 230000037396 body weight Effects 0.000 description 9
- 230000036541 health Effects 0.000 description 9
- 239000000843 powder Substances 0.000 description 9
- 238000005303 weighing Methods 0.000 description 9
- 208000008589 Obesity Diseases 0.000 description 8
- 238000002835 absorbance Methods 0.000 description 8
- 238000011156 evaluation Methods 0.000 description 8
- 235000013312 flour Nutrition 0.000 description 8
- 102000010907 Cyclooxygenase 2 Human genes 0.000 description 7
- 108010037462 Cyclooxygenase 2 Proteins 0.000 description 7
- 235000006708 antioxidants Nutrition 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- XEYBRNLFEZDVAW-ARSRFYASSA-N dinoprostone Chemical compound CCCCC[C@H](O)\C=C\[C@H]1[C@H](O)CC(=O)[C@@H]1C\C=C/CCCC(O)=O XEYBRNLFEZDVAW-ARSRFYASSA-N 0.000 description 6
- 208000027866 inflammatory disease Diseases 0.000 description 6
- 239000004615 ingredient Substances 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 235000020824 obesity Nutrition 0.000 description 6
- 235000011888 snacks Nutrition 0.000 description 6
- 241000282472 Canis lupus familiaris Species 0.000 description 5
- 241000287828 Gallus gallus Species 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 5
- 244000184734 Pyrus japonica Species 0.000 description 5
- 229960002986 dinoprostone Drugs 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- XEYBRNLFEZDVAW-UHFFFAOYSA-N prostaglandin E2 Natural products CCCCCC(O)C=CC1C(O)CC(=O)C1CC=CCCCC(O)=O XEYBRNLFEZDVAW-UHFFFAOYSA-N 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 244000068988 Glycine max Species 0.000 description 4
- 235000010469 Glycine max Nutrition 0.000 description 4
- 240000006240 Linum usitatissimum Species 0.000 description 4
- 235000004431 Linum usitatissimum Nutrition 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 239000002775 capsule Substances 0.000 description 4
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 210000002540 macrophage Anatomy 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 108010010803 Gelatin Proteins 0.000 description 3
- 206010033307 Overweight Diseases 0.000 description 3
- 206010003246 arthritis Diseases 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 230000008030 elimination Effects 0.000 description 3
- 238000003379 elimination reaction Methods 0.000 description 3
- 229920000159 gelatin Polymers 0.000 description 3
- 239000008273 gelatin Substances 0.000 description 3
- 235000019322 gelatine Nutrition 0.000 description 3
- 235000011852 gelatine desserts Nutrition 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- 206010012289 Dementia Diseases 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 238000005481 NMR spectroscopy Methods 0.000 description 2
- 241000015177 Saccharina japonica Species 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000028709 inflammatory response Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000036651 mood Effects 0.000 description 2
- 208000015122 neurodegenerative disease Diseases 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 244000298800 Actinidia arguta Species 0.000 description 1
- 235000016416 Actinidia arguta Nutrition 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000080715 Alexania Species 0.000 description 1
- 208000019901 Anxiety disease Diseases 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 241000209763 Avena sativa Species 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 239000005996 Blood meal Substances 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 238000012286 ELISA Assay Methods 0.000 description 1
- 241000946389 Ecklonia kurome Species 0.000 description 1
- 241001392689 Ecklonia maxima Species 0.000 description 1
- 241000439005 Ecklonia stolonifera Species 0.000 description 1
- 241000243681 Eisenia bicyclis Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 244000052355 Hydrilla verticillata Species 0.000 description 1
- 206010034203 Pectus Carinatum Diseases 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- JAWMENYCRQKKJY-UHFFFAOYSA-N [3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-ylmethyl)-1-oxa-2,8-diazaspiro[4.5]dec-2-en-8-yl]-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]methanone Chemical compound N1N=NC=2CN(CCC=21)CC1=NOC2(C1)CCN(CC2)C(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F JAWMENYCRQKKJY-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019730 animal feed additive Nutrition 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000003429 antifungal agent Substances 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 230000036506 anxiety Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229940036811 bone meal Drugs 0.000 description 1
- 239000002374 bone meal Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000003412 degenerative effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 239000000469 ethanolic extract Substances 0.000 description 1
- 239000002038 ethyl acetate fraction Substances 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 238000004508 fractional distillation Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000003814 hair luster Effects 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 201000008482 osteoarthritis Diseases 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 235000013324 preserved food Nutrition 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- QEVHRUUCFGRFIF-MDEJGZGSSA-N reserpine Chemical compound O([C@H]1[C@@H]([C@H]([C@H]2C[C@@H]3C4=C(C5=CC=C(OC)C=C5N4)CCN3C[C@H]2C1)C(=O)OC)OC)C(=O)C1=CC(OC)=C(OC)C(OC)=C1 QEVHRUUCFGRFIF-MDEJGZGSSA-N 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000010288 sodium nitrite Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 208000003265 stomatitis Diseases 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S426/00—Food or edible material: processes, compositions, and products
- Y10S426/805—Pet food for dog, cat, bird, or fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a composition for animal feed having antioxidant and anti-inflammatory properties, which contains fucoidan extracted from brown algae, and more particularly, to a composition and a product that can be safely fed in daily life by using a raw material derived from natural sources and have functions of improving the luster, smell of feces, color of feces, and overweight of the body.
Description
Technical Field
The present invention relates to a composition for animal feed and an animal product containing brown algae polyphenols having antioxidant and inflammation-improving effects as an active ingredient. The composition and the animal product of the present invention have the function of imparting gloss to animal hair, improving odor, fecal color, and obesity of animals, and contributing to mental stability, through an antioxidant effect, an inflammation-improving effect, and the like. The present invention also relates to an animal feed composition and an animal product which are capable of being safely taken for a long period of time by using natural ingredients as main raw materials.
Background
As the number of single households increases and the average income increases, so does the number of households that feed companion animals. The scale of the related industries is also increasing as the number of individuals of companion animals increases, and according to the current state of the companion animal feed industry of korea rural economic institute, the scale of the feed industry increases from 2500 billion won in 2012 to 4600 billion won in 2016. Also, by improving the awareness of the families that feed companion animals, vaccination is increased and the quality of food is improved, and thus the average life span of companion animals also tends to increase.
However, as companion animals ingest excessive amounts of feed and snacks and ingest food for human consumption, problems arise due to overweight or nutritional imbalances of the body. The increase in animal body weight stimulates joints, and persistent stimulation will cause inflammatory diseases and degenerative arthritis. The occurrence of arthritis and inflammatory diseases induces psychological anxiety during the lifetime of the companion animal and may also negatively affect the mood of the companion animal's owner. Accordingly, the owners of companion animals have increased their attention to degenerative diseases caused by aging such as arthritis and dementia of companion animals, and also have increased their attention to daily health management.
Although inflammation is caused by pathogenic bacteria, stimuli, and the like, and has a function of inhibiting initial cell damage and tissue regeneration, when inflammation becomes severe, diseases such as inflammatory diseases are also induced. In order to prevent inflammation from progressing to disease, immunity needs to be enhanced, which means that nutrient and functional food need to be taken at ordinary times. In addition, in the case of animals, since the meaning cannot be expressed as in humans, it is necessary to take a daily measure to put a functional food having antioxidant and anti-inflammatory functions into a feed or snack to be taken at ordinary times.
Observing the prior art related to animal feeds, Korean patent laid-open No. 10-2005-0099436 provides an animal feed additive containing Actinidia arguta extract having anti-allergic and anti-inflammatory activities, to which Laminaria japonica was added, was granted.
Unlike the conventional patents, the present invention has an object to provide a composition for animal feed containing brown algae polyphenols derived from brown algae and having antioxidant and anti-inflammatory functions, and an animal product containing the functional material.
Disclosure of Invention
Technical problem
The present invention aims to provide a composition for animal feed and an animal product containing brown algae polyphenols as an active ingredient, which have antioxidant and anti-inflammatory functions, and which contribute to the daily health management of animals, reduce the occurrence of inflammatory diseases, and enhance the satisfaction during the lifetime of animals. Further, the present invention has an object to provide various products such as powder type products, animal feeds, snacks, etc. so that users can conveniently select and use them.
Technical scheme
In order to achieve the above object, the present invention provides a composition for animal feed comprising brown algae polyphenols as an active ingredient.
Also, the present invention provides an animal product comprising the above composition.
Also, the present invention provides a method for using a composition comprising brown algae polyphenols as an effective ingredient as an animal feed.
ADVANTAGEOUS EFFECTS OF INVENTION
The composition for animal feed containing brown algae polyphenol as an active ingredient of the present invention contains naturally occurring materials and does not contain preservatives or chemicals, and therefore, can be safely used without fear of side effects and can be used in various forms, for example, feeds, snacks, and the like. Moreover, the product of the invention is suitable for daily use, thereby being beneficial to daily health management of animals and having the effect of enhancing immunity.
Drawings
Fig. 1 is a diagram showing a product example 1 of example 2.
Fig. 2 is a diagram showing product example 2 of example 3.
Fig. 3 is a graph showing the antioxidant effect of composition 2.
Fig. 4 is a graph showing the effect of composition 2 on the inhibition of Nitric Oxide (NO) production.
Fig. 5 is a graph showing the effect of composition 2 on inhibiting prostaglandin E2(PGE2) production.
FIG. 6 is a graph showing the effect of composition 2 on inhibiting the production of cyclooxygenase-2 (COX-2).
Detailed Description
Hereinafter, the present invention will be described in detail.
As the number of families per individual increases, the number of families for raising companion animals increases, and the survival age increases due to the development of feeds and medicines for companion animals, and thus problems relating to health management of older companion animals are highlighted. Degenerative and inflammatory diseases such as arthritis, stomatitis, and dementia, which occur in older companion animals, induce discomfort during lifetime, and may also adversely affect the mood of the person who is keeping the companion animal. Also, obesity and nutritional imbalances induced by irregular nutrient supply to companion animals can worsen the health of the animal.
Accordingly, the present invention has been accomplished by providing a composition for animal feed having anti-inflammatory and antioxidant activities and developing a product using the composition.
The present invention provides a composition for animal feed comprising a brown algae polyphenol compound as an active ingredient.
The composition for animal feed of the present invention comprising brown algae polyphenols as an active ingredient has antioxidant and anti-inflammatory functions, thereby improving the health of animals.
The present invention provides a method for using a composition comprising brown algae polyphenols as an active ingredient as an animal feed. When the composition is used as a feed, the composition improves the health of animals by having antioxidant and anti-inflammatory functions.
The composition for animal feed may comprise 20 to 100 wt% of the brown algae polyphenol compound.
The above-mentioned composition for animal feed as an ingredient derived from natural sources can be obtained by all conventional methods. That is, it can be synthesized by using commercially available reagents, or can be obtained by extracting and isolating from natural products, particularly, from marine algae.
The composition for animal feed may be seaweed extract. The composition for animal feed may be ethanol extract of seaweed.
The composition for animal feed containing the above-mentioned brown algae polyphenol as an active ingredient can be extracted from seaweeds, particularly, seaweeds, and more specifically, seaweeds selected from the group consisting of alexania japonica (Eisenia bicyclis), seaweeds of related good (eiseniaca), Eisenia desmaris, Eisenia gallapagenisis, Eisenia massonii, laminaria japonica (Ecklonia kurome), Ecklonia cava (Ecklonia cava), hydrilla nigra (Ecklonia stolonifera), algea megahominis (Ecklonia maxima), Ecklonia japonica (Ecklonia radiata), Ecklonia japonica (Ecklonia bicyclis), Ecklonia japonica (Ecklonia purpurata), Ecklonia japonica, Ecklonia benthamiana, Ecklonia kura, Ecklonia kulonia, Echinia, Echin.
The composition of the present invention may contain 0 to 80% by weight of components consisting of mannitol, pectic acid, fucoidan, laminaran as carbohydrate components, and fucosterol and fucoxanthin as lipid components, in addition to fucoidan.
The composition for animal feed of the present invention may include one or more brown algae polyphenols selected from the group consisting of compounds represented by the following chemical formulas 1 to 12.
Chemical formula 1:
chemical formula 2:
chemical formula 3:
chemical formula 4:
chemical formula 5:
chemical formula 6:
chemical formula 7:
chemical formula 8:
chemical formula 9:
chemical formula 10:
chemical formula 11:
chemical formula 12:
the composition for animal feed of the present invention may include one or more brown algae polyphenols selected from the group consisting of chemical formula 2, chemical formula 4, chemical formula 5, and chemical formula 8 as an active ingredient. When the brown algae polyphenol is contained in a proper amount, the effects of resisting oxidation and improving inflammation are excellent.
Preferably, the composition for animal feed of the present invention may include 1 to 60 weight percent of brown algae polyphenol of chemical formula 2, 0.3 to 20 weight percent of brown algae polyphenol of chemical formula 4, 0.1 to 10 weight percent of brown algae polyphenol of chemical formula 5, or 0.1 to 5 weight percent of brown algae polyphenol of chemical formula 8, with respect to the total weight.
The composition for animal feed of the present invention may include 0.1 to 3% by weight of brown algae polyphenol of chemical formula 1, 5 to 63% by weight of brown algae polyphenol of chemical formula 2, 1 to 3% by weight of brown algae polyphenol of chemical formula 3, 0.5 to 34% by weight of brown algae polyphenol of chemical formula 4, 0.1 to 50% by weight of brown algae polyphenol of chemical formula 5, 0.5 to 8% by weight of brown algae polyphenol of chemical formula 6, 0.1 to 1% by weight of brown algae polyphenol of chemical formula 7, 0.1 to 46% by weight of brown algae polyphenol of chemical formula 8, 0.1 to 2% by weight of brown algae polyphenol of chemical formula 9, 0.1 to 7% by weight of brown algae polyphenol of chemical formula 10, 0.1 to 3 weight percent of the brown algae polyphenol of chemical formula 11 and 0.1 to 4 weight percent of the brown algae polyphenol of chemical formula 12.
The composition of the present invention may comprise 20 to 100 weight percent of fucoidan, 0 to 30 weight percent of mannitol, 0 to 25 weight percent of pectic acid, 0 to 25 weight percent of fucoidan sulfate, 0 to 10 weight percent of fucosterol, 0 to 10 weight percent of fucoxanthin, and 0 to 10 weight percent of laminaran.
The composition of the present invention may comprise 20 to less than 100 weight percent of fucoidan, more than 0 to 30 weight percent of mannitol, more than 0 to 25 weight percent of pectic acid, more than 0 to 25 weight percent of fucoidan, more than 0 to 10 weight percent of fucosterol, more than 0 to 10 weight percent of fucoxanthin, and more than 0 to 10 weight percent of laminaran.
The composition for animal feed of the present invention may be in the form of powder or liquid for easy addition to products such as animal feed and animal treats.
The present invention provides an animal product comprising a composition for animal feed, wherein the composition for animal feed comprises the above-mentioned brown algae polyphenol.
The animal product of the present invention contains, as an auxiliary ingredient, various adjuvants such as amino acids, inorganic salts, vitamins, antibiotics, antibacterial substances, antioxidants, antifungal agents, enzymes, and active microbial agents, in addition to the animal feed composition containing fucoidan; cereals, for example, crushed or broken wheat, oat, barley, corn, rice, etc.; vegetable proteins such as rape, soybean, sunflower and the like; animal proteins such as blood meal, meat meal, bone meal, and fish meal; and sugar and dairy products such as various milk powders and whey powders.
Preferably, the animal product may include 0.1 to 50 weight percent of the composition for animal feed containing brown algae polyphenols, more preferably, the animal product may include 0.2 to 10 weight percent of the composition for animal feed containing brown algae polyphenols, but the blending amount is not limited to the above range. When the animal product of the present invention contains the composition for animal feed in the above-mentioned content, the antioxidant effect and the anti-inflammatory effect can be imparted to animals, and the composition contributes to health management and can enhance immunity.
In addition to the above composition for animal feed, the present invention provides an animal product comprising one or more selected from the group consisting of collagen, linseed extract, calcium, iron, vitamins such as vitamin B12 and vitamin E, copper, chicken, pig gelatin, rice flour, soybean flour, wheat flour, minerals, chicken oil, sorbitol, propylene glycol, glycerin, fish, and water.
Preferably, the above product may comprise 1 to 50 weight percent of the composition for animal feed of the present invention, 40 to 80 weight percent of collagen, 1 to 4 weight percent of linseed extract, 5 to 15 weight percent of calcium, 0.1 to 0.8 weight percent of iron, 0.008 to 0.01 weight percent of vitamin B12, 0.1 to 0.3 weight percent of vitamin E, and 0.05 to 0.1 weight percent of copper. In this case, there is an effect of reducing the occurrence of inflammatory diseases and enhancing the health of animals.
The animal product of the present invention can be made into products such as animal feed in powder or pellet form or snacks such as dog's teeth bars and cans.
The animal product of the present invention may include 80 to 97% by weight of one or more selected from the group consisting of chicken, pig gelatin, rice flour, soybean flour, and wheat flour. In this case, there is an advantage in that the animal is provided with a balanced nutritional diet and is easily produced.
Preferably, the amount of the brown algae polyphenol contained in the animal product of the present invention is about 0.1mg to 100mg, more preferably about 1mg to 50mg per day, but the amount is not limited to the above range.
Modes for carrying out the invention
Hereinafter, the present invention will be described in more detail by examples and experimental examples. However, the following examples and experimental examples are intended to more specifically illustrate the present invention, and the scope of the present invention is not limited to the following examples and experimental examples. The following examples and experimental examples may be appropriately modified and changed by those skilled in the art within the scope of the present invention as claimed.
Example 1 preparation of compositions containing Brown algae Polyphenol
In order to prepare a composition for animal feed containing the brown algae polyphenol of the present invention as an active ingredient, extraction from korean-produced brown algae was performed by the following method.
Washing thallus laminariae with distilled water to remove foreign substances, drying in shade, and crushing. Adding 500g of above Ecklonia cava into an extraction container, adding 20 times of 30 weight percent ethanol water solution relative to the weight of above Ecklonia cava, and extracting under reflux at 75-80 deg.C for 4 hr to obtain extractive solution. The extract was filtered through a 10 μm filter screen, and the filtered extract was concentrated under reduced pressure to obtain a first extract concentrate. The first extract concentrate was dried and pulverized by a vacuum drier to obtain 170g of composition 1.
Dissolving the above composition 1 in warm water of 50 deg.C, adsorbing on adsorbent resin, washing with water, and dissolving with ethanol to obtain brown algae polyphenol solution. Filtering the above eluate with 1 μm filter screen, and concentrating the filtered extract under reduced pressure to obtain a second concentrated extract. The second extract concentrated solution was dried and pulverized by a vacuum drier to obtain 30g of composition 2.
Then, composition 2 was suspended in a 20-fold amount of distilled water and extracted 3 times with the same amount of ethyl acetate solvent to obtain ethyl acetate fraction. The ethyl acetate was concentrated under reduced pressure by fractional distillation, and the concentrate was dried and pulverized by a vacuum drier to obtain 10g of composition 3.
The compounds were separated on liquid chromatography by loading (loading) the compositions 1 to 3 prepared in the above. In this case, it is confirmed that the obtained compounds are the compounds of chemical formulae 1 to 12 by using Nuclear Magnetic Resonance (NMR) as a compound structure analysis apparatus. The prepared above composition was dissolved in 60 volume percent methanol and filtered using 0.2 μm membrane filter paper, and the filtrate was loaded on high performance liquid chromatography. In high performance liquid chromatography, analysis was performed by supplying 15 to 70 volume percent of methanol at a flow rate of 1.0 ml/min for 30 minutes and applying a linear gradient (linear) using HP ODS Hypersil as a column and distilled water and methanol as a solvent. As a result, the presence of the compounds of the above chemical formulas 1 to 12 was confirmed. The chemical compositions of the above compositions 1 to 3 are shown in table 1 below.
TABLE 1
Example 2 preparation of powder type product example 1 Using composition
As an example of a powder type product using the composition of example 1, product example 1 was prepared by mixing collagen, linseed extract, calcium, iron, vitamin B12, vitamin E, and copper in composition 2. The composition of product example 1 is shown in table 2 below. Example 1 of the product prepared is shown in fig. 1.
TABLE 2
| Name of ingredient | Content (%) |
| Composition 2 | 5 |
| Collagen protein | 75 |
| Linseed extract | 4 |
| Calcium carbonate | 15 |
| Iron | 0.7 |
| Vitamin B12 | 0.01 |
| Vitamin E | 0.2 |
| Copper (Cu) | 0.09 |
| Total up to | 100 |
The product example 1 can be fed to animals as they are, or can be mixed with ordinary feed for use. In addition, the product can be easily mixed with snack such as canned food and chicken breast.
EXAMPLE 3 preparation of product example 2
Animal feed (product example 2) was prepared by adding other raw materials to the product example 1, and the composition thereof is shown in table 3 below. The product example 2 can be fed like ordinary feed, and the feed amount of an animal weighing less than 15kg is 1.5g to 3g per time, the feed amount of an animal weighing 15kg or more and less than 30kg is 2.5g to 5g, and the feed amount of an animal weighing 30kg or more is preferably 5g to 7.5 g. The prepared animal feed is shown in fig. 2.
TABLE 3
| Name of ingredient | Content (%) |
| Product example 1 | 8 |
| Hydrolyzed chicken | 6 |
| Pig gelatin | 5 |
| Rice flour | 33 |
| Soybean powder | 15 |
| Wheat flour | 4 |
| Vitamins and minerals | 0.1 |
| |
3 |
| Sorbitol and propylene glycol | 17.3 |
| Glycerol | 2.6 |
| Corner | 2 |
| Water (W) | 4 |
| Total up to | 100 |
Experimental example 1 evaluation experiment of antioxidant Effect of composition
The antioxidant effect of the composition of example 1 was evaluated as follows.
Composition 2 of example 1 and vitamin C known to have an antioxidant effect were dissolved in 50% aqueous ethanol solutions to a concentration of 1mg/mL, respectively, and then left to stand for 10 minutes after adding 1, 1-diphenyl-2-picrylhydrazinyl (DPPH) solution at a ratio of 10% with an absorbance of 0.94 to 0.98. After 10 minutes, the radical elimination rate was calculated by measuring the absorbance. The method of calculating the radical elimination rate is as follows.
Radical elimination ratio (%) (initial absorbance-absorbance after 10 minutes)/initial absorbance × 100
The results of experimental example 1 are shown in fig. 3. As a result, as shown in fig. 3, the composition of the present invention has antioxidant activity to the same extent as vitamin C.
Experimental example 2 evaluation of anti-inflammatory Effect of the composition
To confirm the anti-inflammatory effect of the composition on animals, the anti-inflammatory effect was evaluated by measuring the concentrations of nitric oxide, prostaglandin E2 and cyclooxygenase-2 after treatment of Lipopolysaccharide (LPS) in a cell line of mouse macrophages (RAW 264.7).
In the measurement of nitric oxide concentration, an inflammatory response was induced by pre-treating composition 2 of example 1 at each concentration (30. mu.g/mL to 120. mu.g/mL) in cultured cells and treating lipopolysaccharide after 1 hour. After 24 hours of lipopolysaccharide treatment, Griess reagent (Griess reagent) was mixed in an amount equivalent to the culture solution, and absorbance was measured at 530 nm. For the calculation of the nitric oxide concentration, sodium nitrite (NaNO) was used2) The absorbance of each concentration of (a) was plotted as a standard curve, and then the concentration was calculated.
The concentrations of prostaglandin E2 and cyclooxygenase-2 were measured using an enzyme-linked immunosorbent assay (ELISA assay). After treatment of lipopolysaccharide and the composition in cell culture broth, the cells were inoculated in an enzyme-linked immunosorbent kit one day later, and the absorbance was measured at 540 nm. The absorbance results of the standard substance for each concentration were used to draw a standard curve by a 4-parameter logistic curve formula (4-PL logistic curve fit), and then the concentration of each protein was calculated. The anti-inflammatory effect evaluation results are shown in fig. 4 to 6. Significance of the experimental results was confirmed by T-test (T-test) based on P <0.05 (. # P <0.05vs control group (control), # P <0.05vs lipopolysaccharide).
As shown in the experimental results of fig. 4 to 6, it was confirmed that the composition of the present invention significantly reduces the inflammatory reaction due to lipopolysaccharide.
From fig. 4, in the experiment using mouse macrophages, it was confirmed that the concentration of nitric oxide was decreased when the composition of the present invention was treated with lipopolysaccharide, and it was found that the decrease in the concentration of nitric oxide was more significant as the concentration was higher. This means that the composition of the present invention reduces inflammation by inhibiting the production of nitric oxide, which is an inflammatory reaction product caused by lipopolysaccharide.
From fig. 5, in the experiment using mouse macrophages, it was confirmed that the concentration of cyclooxygenase-2 was reduced when the composition of the present invention was treated with lipopolysaccharide, and it was also understood that the reduction in the concentration of cyclooxygenase-2 was more significant as the concentration was higher. This means that the composition of the present invention reduces inflammation by inhibiting the production of cyclooxygenase-2, which is an inflammation reaction protein caused by lipopolysaccharide.
From fig. 6, in the experiment using mouse macrophages, it was confirmed that the concentration of prostaglandin E2 was reduced when the composition of the present invention was treated with lipopolysaccharide, and it was also confirmed that the reduction in prostaglandin E2 concentration was more significant as the concentration was higher. This means that the composition of the present invention reduces inflammation by inhibiting the production of prostaglandin E2, which is an inflammatory response protein caused by lipopolysaccharide.
Experimental example 3 efficacy evaluation experiments of product examples 1 and 2
The samples prepared in the form of capsules from 0.1g of the powder of product example 1 and the samples of product example 2 were continuously fed to the subject animals for 1 month together with the feed. The efficacy of the product examples was evaluated by evaluating the odor, stool color, stool odor, and hair luster of the test subjects before and after feeding. The evaluation method was evaluated using 5 scales, i.e., using 2 (bad), 4, 6, 8, and 10 (good). In order to obtain objective experimental results, only healthy and non-abnormal animals were selected as the subjects, and the species of the subjects was dogs. Product example 1 was mixed with feed and fed 2 times per day according to the weight of the experimental animals, animals weighing less than 15kg fed 1 capsule, animals weighing more than 15kg and less than 30kg fed 2 capsules, and animals weighing more than 30kg fed 3 capsules. Product example 2 animals weighing less than 15kg were fed 1.5g, animals weighing more than 15kg and less than 30kg were fed 2.5g, and animals weighing more than 30kg were fed 5g, mixed with the feed and fed 2 times a day. The evaluation of animals fed product example 1 and product example 2 is shown in table 4 below.
As shown in the experimental results of the table, it was confirmed that the gloss, smell of feces and color of the hair of the fed animals were improved in both the product example 1 and the product example 2 of the present invention.
TABLE 4
Experimental example 4 evaluation of obesity-improving efficacy of product example 2
To evaluate the obesity improvement efficacy of the animal feed of product example 2, 10 dogs that exceeded normal body weight by 20% were selected and mixed product example 2 was fed while feeding the feed. The obesity-improving effect was evaluated by comparing the body weight before and after feeding 1 month after animals having a body weight of less than 15kg fed 1.5g of product example 2, animals having a body weight of 15kg or more and less than 30kg fed 2.5g of product example 2, and animals having a body weight of 30kg or more fed 5g of product example 2. In order to obtain objective experimental results, animals whose growth had been completed were selected as experimental subjects, and animals having no abnormality other than obesity were selected. The body overweight rate of the animals was calculated by the following formula using the standard body weight of each dog.
Body excess ratio (%) - (current weight-normal weight)/normal weight
The results of the evaluation test of the obesity improvement efficacy of product example 2 are shown in table 5.
As shown in the experimental results of the table, it was confirmed that the obesity of the dog fed the product example 2 of the present invention was improved.
TABLE 5
| Classification | Body weight excess (%) | Body weight excess (%) |
| Product example 2 | 20.69 | 8.17 |
Industrial applicability
The present invention uses a composition comprising brown algae polyphenols extracted from brown algae, thereby allowing safe feeding to animals, improving the luster, smell, stool color and body overweight of the animal's fur.
Claims (13)
1. A composition for animal feed, characterized by comprising a brown algae polyphenol compound as an active ingredient.
2. The composition for animal feed according to claim 1, wherein the brown algae polyphenol compound is one or more selected from brown algae polyphenols represented by the following chemical formulae 1 to 12:
chemical formula 1:
chemical formula 2:
chemical formula 3:
chemical formula 4:
chemical formula 5:
chemical formula 6:
chemical formula 7:
chemical formula 8:
chemical formula 9:
chemical formula 10:
chemical formula 11:
chemical formula 12:
3. the composition for animal feed according to claim 1, wherein the composition comprises 20 to 100 wt% of total brown algae polyphenol compounds.
4. The composition for animal feed according to claim 1, wherein the composition comprises at least one of the brown algae polyphenols selected from the group consisting of chemical formula 2, chemical formula 4, chemical formula 5 and chemical formula 8.
5. The composition for animal feed of claim 4, wherein the composition comprises 1 to 60 wt% of the brown algae polyphenol of chemical formula 2, 0.3 to 20 wt% of the brown algae polyphenol of chemical formula 4, 0.1 to 10 wt% of the brown algae polyphenol of chemical formula 5, or 0.1 to 5 wt% of the brown algae polyphenol of chemical formula 8.
6. The composition for animal feed according to claim 1, wherein the composition for animal feed is a seaweed extract.
7. The composition for animal feed according to claim 1, wherein the brown algae polyphenol compound is extracted from at least one marine algae selected from the group consisting of Aisenna, Phaeophyceae, Eisenia desmatrastioides, Eisenia galapagenies, Eisenia masonii, Ecklonia cava, Blueda, Okamura, Ecklonia cava, Ecklonia bicincinate, Palmaria lanuginosa, Phanerochaete gloea, Ecklonia exarata, Ecklonia fartinata, Ecklonia brevis, Ecklonia arborea, Ecklonia latifolia, Ecklonia murantii, Ecklonia radiata, Ecklonia rucaria and Ecklonia wrightiii.
8. The composition for animal feed according to claim 3, comprising 20 to 100% by weight of fucoidan, 0 to 30% by weight of mannitol, 0 to 25% by weight of pectic acid, 0 to 25% by weight of fucoidan sulfate, 0 to 10% by weight of fucosterol, 0 to 10% by weight of fucoxanthin, and 0 to 10% by weight of laminaran.
9. An animal product comprising the composition for animal feed of claim 1.
10. The animal product of claim 9, wherein the product is in the form of an animal feed, a can for an animal, or a dog molar bar.
11. Product for animals according to claim 9, characterized in that it comprises from 0.1 to 50% by weight of said composition relative to the total weight of the product.
12. The animal product of claim 9, further comprising at least one member selected from the group consisting of collagen, flax seed extract, calcium, iron, vitamin B12, vitamin E, and copper.
13. The animal product of claim 9, wherein the product comprises from 1 to 50 weight percent of the polyphenols from brown algae, from 40 to 80 weight percent of the collagen, from 1 to 6 weight percent of the extract from flax seeds, from 0.001 to 18 weight percent of calcium, from 0.1 to 1 weight percent of iron, from 0.00001 to 0.01 weight percent of vitamin B12, from 0.1 to 0.5 weight percent of vitamin E, and from 0.05 to 0.1 weight percent of copper.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20180009440 | 2018-01-25 | ||
| KR10-2018-0009440 | 2018-01-25 | ||
| PCT/KR2019/001025 WO2019147044A1 (en) | 2018-01-25 | 2019-01-24 | Composition for animal feed comprising phlorotannin as active ingredient and product for animal |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111669976A true CN111669976A (en) | 2020-09-15 |
Family
ID=67394672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980009505.5A Pending CN111669976A (en) | 2018-01-25 | 2019-01-24 | Composition for animal feed containing brown algae polyphenol as active ingredient and animal product |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR102192712B1 (en) |
| CN (1) | CN111669976A (en) |
| WO (1) | WO2019147044A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2579600B (en) * | 2018-12-05 | 2023-07-05 | Byotrol Plc | Anti-viral composition |
| KR102389589B1 (en) | 2021-09-29 | 2022-04-22 | 진지영 | Antifungal composition comprising spirits extract of Lespedeza cuneata and process for preparing the same |
| KR20230054016A (en) | 2021-10-15 | 2023-04-24 | 진지영 | Antiseptic composition and process for preparing the same |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010041832A2 (en) * | 2008-10-07 | 2010-04-15 | 부경대학교 산학협력단 | Antioxidant active composition containing an ishige okamurae-derived compound |
| WO2011096688A2 (en) * | 2010-02-03 | 2011-08-11 | 라이브켐 주식회사 | Composition for improving scalp and hair health comprising a dibenzo-p-dioxin derivative |
| CN104829740A (en) * | 2015-05-12 | 2015-08-12 | 上海海洋大学 | Method for synchronously extracting sargassum graminifolium polysaccharide and sargassum graminifolium polyphenol from sargassum graminifolium |
| WO2015178728A1 (en) * | 2014-05-23 | 2015-11-26 | 주식회사 비비에이치씨 | Method for preparing induced pluripotency stem cells from mesenchymal stem cells by using phlorotannin fraction |
| CN109276466A (en) * | 2017-07-21 | 2019-01-29 | 博塔医疗株式会社 | It is effective component for preventing white hair and promoting the composition and product of dark hair using brown algae polyphenols |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4105535B2 (en) * | 2002-12-10 | 2008-06-25 | 熊本県 | Antiviral substance |
| KR20050099436A (en) | 2004-04-10 | 2005-10-13 | 김정성 | A road warning lamp |
| NL2002063C (en) * | 2008-10-06 | 2010-04-07 | Coulisse Bv | SYSTEM OF A NUMBER OF REMOTE CONTROLLABLE SCREENS, SUCH AS WINDOW COVERS, AND A REMOTE CONTROLLER FOR OPERATING THE SCREENS AND A METHOD INTENDED FOR APPLICATION TO THE SYSTEM. |
| KR20100039106A (en) * | 2008-10-07 | 2010-04-15 | 부경대학교 산학협력단 | An anti-allergy active composition containing compounds from ecklonia cava |
| KR101245349B1 (en) * | 2008-10-07 | 2013-03-20 | 부경대학교 산학협력단 | A pharmaceutical composition for inhibiting HIV-1 containing phloroglucinol copolymer compound from Ecklonia cava |
| WO2011115910A1 (en) * | 2010-03-15 | 2011-09-22 | Rutgers, The State University Of New Jersey | Methods of obtaining natural products from comestible fluids and methods of use |
| KR101266889B1 (en) * | 2011-01-13 | 2013-05-24 | 주식회사 보타메디 | Functional food compositions having the recovery effect of blood composition and function |
| KR102006501B1 (en) * | 2012-06-18 | 2019-08-01 | 부경대학교 산학협력단 | Antibiotics Composition Containing Phlorotannins from Eisenia bicyclis |
| KR101542882B1 (en) * | 2013-10-30 | 2015-08-10 | 한국생명공학연구원 | A composition for prevention and treatment of fish viral hemorrhagic septicemia containing extracts of Ecklonia cava |
| KR101845287B1 (en) * | 2015-10-30 | 2018-04-04 | 주식회사 보타메디 | Beverage composition comprising phlorotannin, ginseng extract and caffeine |
| KR101777842B1 (en) | 2016-05-04 | 2017-09-14 | 주식회사 엘에스과학기술원 | Feed with seawater and aweed, and manufacturing method of the same |
| KR101772335B1 (en) * | 2016-07-15 | 2017-09-12 | 성균관대학교산학협력단 | A composition for treating, improving and preventing of papilloma virus infection comprising phlorotannin |
| KR101966124B1 (en) * | 2016-11-10 | 2019-04-05 | 조선대학교산학협력단 | Composition comprising extract of Ecklonia cava or phlorotannin compounds isolated from thereof for preventing or treating influenza |
-
2019
- 2019-01-24 CN CN201980009505.5A patent/CN111669976A/en active Pending
- 2019-01-24 KR KR1020190009205A patent/KR102192712B1/en active IP Right Grant
- 2019-01-24 WO PCT/KR2019/001025 patent/WO2019147044A1/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010041832A2 (en) * | 2008-10-07 | 2010-04-15 | 부경대학교 산학협력단 | Antioxidant active composition containing an ishige okamurae-derived compound |
| WO2011096688A2 (en) * | 2010-02-03 | 2011-08-11 | 라이브켐 주식회사 | Composition for improving scalp and hair health comprising a dibenzo-p-dioxin derivative |
| WO2015178728A1 (en) * | 2014-05-23 | 2015-11-26 | 주식회사 비비에이치씨 | Method for preparing induced pluripotency stem cells from mesenchymal stem cells by using phlorotannin fraction |
| CN104829740A (en) * | 2015-05-12 | 2015-08-12 | 上海海洋大学 | Method for synchronously extracting sargassum graminifolium polysaccharide and sargassum graminifolium polyphenol from sargassum graminifolium |
| CN109276466A (en) * | 2017-07-21 | 2019-01-29 | 博塔医疗株式会社 | It is effective component for preventing white hair and promoting the composition and product of dark hair using brown algae polyphenols |
Non-Patent Citations (1)
| Title |
|---|
| SEOK-CHUN KO等: "Dieckol, a phlorotannin isolated from a brown seaweed, Eckloniacava, inhibits adipogenesis through AMP-activated protein kinase(AMPK) activation in 3T3-L1 preadipocytes", 《ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20190090715A (en) | 2019-08-02 |
| WO2019147044A1 (en) | 2019-08-01 |
| KR102192712B1 (en) | 2020-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111669976A (en) | Composition for animal feed containing brown algae polyphenol as active ingredient and animal product | |
| Abd El-Hack et al. | Pharmacological, nutritional and antimicrobial uses of Moringa oleifera Lam. leaves in poultry nutrition: an updated knowledge | |
| Settle et al. | Effects of a phytogenic feed additive versus an antibiotic feed additive on oxidative stress in broiler chicks and a possible mechanism determined by electron spin resonance | |
| Varricchio et al. | Influence of polyphenols from olive mill wastewater on the gastrointestinal tract, alveolar macrophages and blood leukocytes of pigs | |
| CN108601372A (en) | The effective anti-oxidant compositions of collaboration for pet | |
| Fernández-Navarro et al. | Maslinic acid added to the diet increases growth and protein-turnover rates in the white muscle of rainbow trout (Oncorhynchus mykiss) | |
| Rzepecka-Stojko et al. | The Effect of Storage of Bee Pollen Extracts on Polyphenol Content. | |
| Yilmaz et al. | Effects of dietary reishi mushroom (Ganoderma lucidum) on the growth performance of Nile tilapia, Oreochromis niloticus juveniles | |
| CN106720956A (en) | A kind of carp feed | |
| Mohammadi et al. | Effects of dietary supplementation of date palm (Phoenix dactylifera) seed extract on body composition, lipid peroxidation and tissue quality of common carp (Cyprinus carpio) juveniles based on the total volatile nitrogen test | |
| KR100847354B1 (en) | A method for breeding animal using by the leaf of Pinus Koraiensis and the functional animal meat products using thereby | |
| Olusola et al. | Bioproductive effects of Clarias gariepinus fingerlings fed guava (Psidium guajava) leaves and drumstick (Moringa oleifera) leaves extracts supplemented diet | |
| Lásztity et al. | On the presence and distribution of some bioactive agents in oat varieties | |
| Jha et al. | In vitro evaluation of free radical scavenging activity of Pistia stratiotes | |
| Atlanderova et al. | Biological evaluation of aqueous extract of oak bark on in vitro models | |
| US11825864B2 (en) | Palatable pet food composition | |
| Chedea et al. | Total polyphenols content, antioxidant activity and stability of a grape pomace incorporated in animal feed | |
| EP2052731B1 (en) | Composition for preventing and/or treating itching containing component originating in the bark of tree belonging to the genusacacia | |
| Suwannachot et al. | Supplementation with rice bran hydrolysates reduces oxidative stress and improves lipid profiles in adult dogs | |
| KR102407123B1 (en) | Probiotic composition containing monascus purpureus | |
| Longo et al. | Beneficial effects of natural products: new candidates for feed additives | |
| Ibegbulem et al. | Consumption of green tea infusion causes loss of nutrients through faeces in Wistar rats | |
| You et al. | Isolation of antioxidant with exercise enhancing potential from Pseudosasa japonica leaves | |
| Sikder et al. | Physicochemical quality assessment of salt-free fermented fish products of Sylhet region, Bangladesh | |
| El et al. | Hypoglycemic Effect of AzollaAlgae (Azollacaroliniana) on Rats Injected by Alloxan |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200915 |
|
| WD01 | Invention patent application deemed withdrawn after publication |