KR20200013192A - A cosmetic composition containing a leaf extract of dendropanax morbifera and a extract of hallabong rind - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of a Dendropanax morbiferus leaf extract and a Citrus reticulata peel extract as an active ingredient, and more specifically, to a cosmetic composition for anti-acne function having antioxidant, anti-inflammatory and antibacterial activity. The cosmetic composition can be applied to a variety of cosmetic formulations while having antioxidant, anti-inflammatory and anti-bacterial effects, and thus can secure a high value as a cosmetic material having an anti-acne function.

Description

Cosmetic Composition Containing Hwangchil Tree Leaf Extract and Hallabong Bark Extract {A COSMETIC COMPOSITION CONTAINING A LEAF EXTRACT OF DENDROPANAX MORBIFERA AND A EXTRACT OF HALLABONG RIND}

The present invention relates to a cosmetic composition containing a mixed extract of Hwangchil-tree leaf extract and Hallabong bark extract as an active ingredient, and more particularly, to an anti-acne functional cosmetic composition having antioxidant, anti-inflammatory and antibacterial activity.

Acne is an inflammatory disease of the sebaceous glands, characterized by the formation of scalp, erythematous papules, pustules, etc., and may leave a lacuna or hypertrophic scar scar. The exact cause of acne is not known, but various factors are involved.

The etiology of acne is caused by hyperkeratosis of the hair follicle wall and abnormal dropout, comedogenesis, increased sebum production by androgen, proliferation of acne bacteria (Propionibacterium acnes), and inflammation. , Skin barrier function abnormality, stress, heredity, cosmetics and face washing, menstruation and hormones, season, environmental factors, hair follicle reactivity. Among the major causes of acne is the proliferation of acne-causing bacteria, Propionibacterium acnes. The bacterium bacterium Propionibacterium acne is a major cause of troubles when eating and growing sebum.In normal people, even if the bacteria are present on the skin, they do not cause any problems, but since sebum is clogged up and sebum accumulates in the hair follicles Eating and growing fast

The acne-causing bacteria of the propionibacterium acne is to eat fat (triglycerides) and then excrete butter-like substances, that is, fatty acids. These chemical changes cause healthy weak acidic skin to alkalinize as the pH balance of oil breaks down, which in turn degrades the skin's own functions and causes a vicious cycle of the skin. In order to prevent and alleviate the acne caused by Propionibacterium acne, methods of treating conventionally applied medicines and medicines have been proposed.

On the other hand, Dengropanas morbifera is an evergreen broad-leaved forest belonging to the family Araliaceae, and is a subtropical species that grows and grows only in the southwestern coast and the island of Jeonnam. Hwangchil's resin is golden, heat resistant, durable, water resistant, and has good adhesion and gloss. It has been used as a natural paint and folk medicine material in traditional crafts since ancient times. In addition, Hwangchil contains not only essential oils such as sesquiterpene, but also various ingredients such as arachidic acid, palmitic acid, and arginine, and has effects such as atherosclerosis, liver function improvement, antioxidant bone regeneration, immune enhancement, antibacterial activity and anticancer effect. Is being reported.

Hallabong (Citrus unshiu Marcov X C. sinensis Osbeck) X C. reticulate Blanco is a hybrid of citrus fruits grown in Cheongdo and Fengcan at the Japanese Ministry of Agriculture, Forestry and Orchards. The pulp has a sweetness of 15 brix, has no seeds, and has a characteristic protrusion on the upper part of the pulp, with an average diameter of 6.5-9.7 cm. Recently, Hallabong rind contains abundant components such as essential oils, polymetoxyflavones and polysaccharides, which are chemical components that have anti-cancer and anti-inflammatory effects. It turns out that it works. In addition, Hallabong's skin and fruit contains β-cryptoxanthin, which is known to have antioxidant properties as a precursor of vitamin A and inhibit free radical-induced cell and DNA damage. Recently, in order to develop cosmetic raw materials for improving acne, the search for an antioxidant active substance that removes free radicals, which are active oxygen species, and the development of substances that alleviate the antibacterial activity against acne bacteria and inflammation of the skin are being actively made.

Korean Patent Publication No. 10-2011-0082292 Korean Patent Publication No. 10-2018-0011748

An object of one embodiment of the present invention is to provide a cosmetic composition for anti-acne functionality containing a mixed extract of the Hwangchil-tree leaf extract and Hallabong bark extract.

One embodiment of the present invention provides a cosmetic composition containing a mixed extract of the Hwangchil-tree leaf extract and Hallabong bark extract as an active ingredient.

The cosmetic composition may contain 0.1 to 80% by weight based on the total weight.

The cosmetic composition may be performed to scaveng DPPH radicals.

The cosmetic composition may be performed to scaveng ABTS radicals.

The cosmetic composition may increase the antioxidant activity.

The cosmetic composition may have a nitric oxide (Nitric Oxide) generation inhibitory ability.

The cosmetic composition according to one embodiment of the present invention may have a formulation selected from the group consisting of solution, gel, cream, essence, lotion, soap, foam cleansing, mist and serum.

The cosmetic composition according to one embodiment of the present invention may be an anti-acne functional having antioxidant, anti-inflammatory and antibacterial activity.

Cosmetic composition comprising a mixed extract of the Hwangchil-tree leaf extract and Hallabong bark extract of the present invention can be applied to a variety of cosmetic formulations, while having high antioxidant, anti-inflammatory and antimicrobial effects to secure a high value as a cosmetic having an acne function Can be.

1 is a photograph of the measurement of acne bacteria inoculation concentration of the Hwangchil-tree leaf extract, Hallabong bark extract and the mixed extract of Hwangchil-tree leaf and Hallabong bark.
Figure 2 is a graph showing the NO production rate of the mixed extract of Hwangchil-tree leaves and Hallabong bark in RAW 264.7 cells.
Figure 3 is a graph showing the PGE ₂ content of the mixed extract of Hwangchil-tree leaves and Hallabong bark in RAW 264.7 cells.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art. Accordingly, embodiments of the present invention may be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below.

Throughout the specification of the present invention, when a part is said to "include" a certain component, it means that it can further include other components, without excluding the other components unless otherwise stated.

Throughout the description of the present invention, when a step is located "on" or "before" another step, it is not only when a step is in direct time-series relationship with the other step, but also after mixing with each step. Likewise, the order of the two phases may include the same rights as in the case of an indirect time series relationship in which the time series order can be reversed.

The present invention relates to a cosmetic composition containing a mixed extract of Hwangchil-tree leaf extract and Hallabong bark extract as an active ingredient, while the cosmetic composition according to the present invention is applicable to various cosmetic formulations, and has anti-oxidant, anti-inflammatory and antibacterial effects. High value as a cosmetic having acne function can be ensured.

In particular, the cosmetic composition of the present invention may exhibit more excellent anti-inflammatory activity and anti-acne bactericidal activity by containing both Hwangchil-tree leaf extract and Hallabong bark extract.

Hwangchil tree of the present invention reaches a height of 15 m and young branches are green and hairless. The leaves are alternate and egg-shaped or oval. In addition, the leaf edge is flat, but in young trees, it is divided into 3 ~ 5 and serrated. Flower is pale yellow-green in June, bisexual, and hangs on inflorescence. A stalk is 3 ~ 5 cm long and a small stalk is 5 ~ 10 mm long. The calyx is divided into 5 pieces, the petals and stamens are 5 pieces, and the flower bed has a honey gland. The pistil is divided into 5, the oval is elliptical, and matures in October, and the style remains. When the bark is cut, yellow liquid comes out. This is called Hwangchil and used as paint for furniture. Indigenous to Korea, it grows on southern islands.

In the present invention, the hwangchil-tree leaf extract is obtained by extracting and fermenting from various organs or leaves, flowers, roots, stems, branches, shells, sap and seeds of the hwangchil tree, preferably leaves, stems, branches, Fermented extract obtained from the bark, sap or root, more preferably fermented extract obtained from the leaf, stem or sap, and most preferably means the fermented extract obtained from the leaf or sap.

Hallabong of the present invention is a variety of hybrid citrus fruit that was bred by breeding Kiyomi and Ponkan in Japan in 1972. The Japanese variety name is shiranui, the product is distributed under the brand name "dekopon", and is cultivated in Brazil in large units. Introduced around 1990, Korea has begun shipping the name of various varieties such as localization and deco-phone for each cultivated farm, and the name of Hallabong citrus faucet sticking out resembles Hallabong because it is similar to Hallasan. Got Hallabong's young tree grows straight, but splits laterally, and the flowers are larger and less pollen than other citrus flowers. The fruit is almost seedless, with very few seeds. The fruit weighs about 200-300 g and varies from the tip of the stalk to the end of the fruit, not from the tip, and is generally uneven in size and shape. It was initially pale green, but began to change color in mid-October, turning orange in early December and ripening in February-March. The shell is 3.5 ~ 5 mm thick, relatively thin in size, and rough but peels off. High in sugar, tender in meat and juicy. The shell contains a lot of essential oils, various carotenoids, flavonoids and various vitamins, and especially limonene (α-limonene), which accounts for most of the essential oils (70-90%), calms the excitement of the central nervous system and has anticancer activity. This is known to be. It is widely used in cosmetics and food industry because of its refreshing aroma and non-toxic to human body.

Hwangchil-tree leaf extract and Hallabong bark extract of the present invention can be prepared by using water, ethanol or a mixed solvent thereof as an extraction solvent, respectively. It is preferable to use a mixed solvent of water and ethanol, more preferably 30 to 70% (v / v) ethanol, and more preferably 40 to 60% (v / v) ethanol. At this time, the extraction temperature is preferably 0 ~ 90 ℃, more preferably 20 ~ 50 ℃. The extraction time on the extraction temperature is preferably 1 to 5 days, more preferably 2 to 4 days.

The cosmetic composition of the present invention is not limited to being prepared in any formulation commonly prepared in the art, for example, formulated into a solution, gel, cream, essence, lotion, soap, foam cleansing, mist, serum, and the like. It may be, but is not limited to such. More specifically, in the case of a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed or washed with water. As a specific example, the soaps are liquid soaps, powdered soaps, solid soaps and oil soaps, the surfactant-containing cleansing formulations are cleansing foams, cleansing water, cleansing towels and cleansing packs, and the surfactant-free cleansing formulations are cleansing creams. , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

Hereinafter, the present invention will be described in detail according to specific examples. However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

Example 1 Preparation of Hwangchil Tree Leaf Extract

Hwangchil tree leaves were ground using a grinder. Hwangchil-tree leaf extract of the present invention was added to 10g multiples of 5g crushed hwangchil leaves according to the ratio of the extraction solvent described in the following [Table 1] 50ml each solution was shaken and filtered for 72 hours at 35 ℃. .

menstruum Mixing ratio DDW (%) 100 50 0 EtOH (%) 0 50 100

Example 2. Preparation of Hallabong Bark Extract

Hallabong husks were powdered using a grinder. The Hallabong bark extract of the present invention was added to 10 g multiples of 5 g of Hallabong bark pulverized according to the ratio of the extraction solvent described in the following [Table 2] and 50 ml of the solution were shaken and filtered at 35 ° C. for 72 hours. .

DDW (%) 100 50 0 EtOH (%) 0 50 100

Experimental Example 1. Antioxidant activity of Hwangchil-tree leaf extract, Hallabong bark extract, and the mixed extract of Hwangchil-tree leaf and Hallabong bark

Experimental Example 1-1. DPPH radical scavenging activity

DPPH radical scavenging activity was measured for the extracts of Hwangchil-tree leaf extract, Hallabong bark extract, and Hwangchil-tree leaf and Hallabong bark extract prepared in Examples 1 and 2 as follows. That is, the DPPH assay method is Yoshida et al. (1989) according to the method used. In a 96well plate, 100 μL of the extracted solvent was added to the control, and 100 μL of the yellow-leaf tree extract was added to the test group, and 100 μL was added to all wells. 50 μL of DPPH (0.5 mM 1,1-diphenyl-2-picrylhydrazyl was added. (DPPH) / ethanol) solution was added and mixed. Absorbance was measured at 517 nm after reaction at room temperature of 25 ° C. for 30 minutes. The radical scavenging ability of the extract relative to the control with the addition of solvent is shown as a percentage.

The results are shown in Table 3 below.

Sample DPPH radical scavenging activity (%) Yellow leaves
50% Ethanol Extract Stock 97.59 ± 0.27 2 times dilution 97.16 ± 0.07 4 times dilution 83.74 ± 0.25 8 times dilution 82.04 ± 0.34 16 times dilution 76.11 ± 0.75 32 times dilution 49.19 ± 4.00 64 times dilution 27.89 ± 1.17 128-fold dilution 14.33 ± 4.99 Hallabong Shell Water Extract Stock 87.73 ± 0.34 2 times dilution 87.72 ± 0.32 4 times dilution 86.08 ± 0.11 8 times dilution 74.05 ± 1.66 16 times dilution 36.98 ± 1.99 32 times dilution 21.27 ± 0.81 64 times dilution 5.27 ± 1.02 128-fold dilution 0.24 ± 1.11 Hallabong Shell
50% Ethanol Extract Stock 82.64 ± 0.43 2 times dilution 80.19 ± 0.62 4 times dilution 73.25 ± 2.29 8 times dilution 51.85 ± 1.83 16 times dilution 45.55 ± 1.06 32 times dilution 30.70 ± 0.21 64 times dilution 24.15 ± 1.52 128-fold dilution 15.88 ± 2.67 Hallabong Shell
100% Ethanol Extract Stock 91.82 ± 0.71 2 times dilution 91.00 ± 1.16 4 times dilution 67.84 ± 0.84 8 times dilution 42.76 ± 0.51 16 times dilution 27.88 ± 1.01 32 times dilution 20.35 ± 1.84 64 times dilution 16.57 ± 2.25 128-fold dilution 16.00 ± 0.52 Yellow leaves and
Hallabong Shell
Mixed solution Stock 86.70 ± 5.06 2 times dilution 86.37 ± 2.02 4 times dilution 85.99 ± 0.54 8 times dilution 84.06 ± 0.26 16 times dilution 77.11 ± 2.17 32 times dilution 56.70 ± 6.11 64 times dilution 28.88 ± 2.17 128-fold dilution 12.50 ± 1.37

As shown in the results of Table 3, in the preliminary study, the hwangchil tree leaves the best antioxidant activity in 40 ~ 60% ethanol extract, measured the antioxidant activity for 50%, search for the optimal extraction conditions of Hallabong bark extract For this purpose, the ethanol and water mixtures were extracted and the antioxidant activities of the extracts were compared. As a result, the antioxidant activity was highest when the mixing ratio of ethanol and water is 50:50. When antioxidant activity was measured with a mixture of Hwangchil-tree leaf and Hallabong bark extract 1: 1, it showed slightly higher antioxidant activity than that of Hwangchil-tree leaf extract alone.

Hallabong bark extract has a lower antioxidant activity than Hwangchil tree leaf extract. In the case of the mixed extract of Hwangchil-tree leaf and Hallabong bark extract, the ratio of DPPH radical scavenging activity was increased to 28.88%, indicating that there was DPPH radical scavenging activity.

Experimental Example 1-2. ABTS radical scavenging ability

ABTS radical scavenging activity was measured for the extracts of Hwangchil-tree leaf extract, Hallabong bark extract, and Hwangchil-tree leaf and Hallabong bark extract prepared in Examples 1 and 2 as follows. That is, ABTS radical scavenging activity is described in Re et al. (1999) was measured by modifying the method. That is, 5 mL of 7 mM ABTS and 88 μL of 140 mM potassium persulfate were mixed and blocked with light at room temperature for 16 hours to form an ABTS cation. The solution was diluted with PBS to have an absorbance value of 1.5 at 414 nm. 190 μL of the prepared dilution solution and 10 μL of the sample were mixed and reacted at room temperature for 6 minutes, and then the absorbance was measured at 734 nm. As a solvent control, the radical scavenging ability of the control was expressed as a percentage.

The results are shown in Table 4 below.

Sample ABTS radical scavenging activity (%) Yellow leaves
50% Ethanol Extract Stock 99.25 ± 0.05 2 times dilution 99.50 ± 0.14 4 times dilution 99.55 ± 0.02 8 times dilution 97.35 ± 1.68 16 times dilution 87.79 ± 2.93 32 times dilution 59.86 ± 2.97 64 times dilution 36.84 ± 3.85 128-fold dilution 23.52 ± 1.34 Hallabong Shell Water Extract Stock 99.78 ± 0.06 2 times dilution 103.23 ± 0.11 4 times dilution 99.90 ± 0.02 8 times dilution 94.54 ± 4.09 16 times dilution 84.12 ± 2.03 32 times dilution 58.36 ± 1.52 64 times dilution 36.12 ± 4.06 128-fold dilution 22.08 ± 0.92 Hallabong Shell
50% Ethanol Extract Stock 99.47 ± 0.10 2 times dilution 99.18 ± 0.16 4 times dilution 88.17 ± 4.81 8 times dilution 62.20 ± 5.76 16 times dilution 35.81 ± 0.91 32 times dilution 24.86 ± 1.64 64 times dilution 18.48 ± 1.87 128-fold dilution 9.87 ± 1.61 Hallabong Shell
100% Ethanol Extract Stock 99.76 ± 0.08 2 times dilution 99.76 ± 0.08 4 times dilution 99.74 ± 0.08 8 times dilution 94.04 ± 3.74 16 times dilution 70.80 ± 3.44 32 times dilution 63.78 ± 2.79 64 times dilution 32.12 ± 2.37 128-fold dilution 19.72 ± 2.21 Yellow leaves and
Hallabong Shell
Mixed solution Stock 99.59 ± 0.01 2 times dilution 99.65 ± 0.06 4 times dilution 99.69 ± 0.09 8 times dilution 97.51 ± 1.21 16 times dilution 86.22 ± 2.01 32 times dilution 67.21 ± 1.35 64 times dilution 39.73 ± 5.35 128-fold dilution 36.13 ± 5.49

As shown in the results of Table 4, in the preliminary study, the hwangchil-tree leaves were the best antioxidant activity in 40 ~ 60% ethanol extract, measured the antioxidant activity for 50%, search for the optimal extraction conditions of Hallabong bark extract For this purpose, the ethanol and water mixtures were extracted and the antioxidant activities of the extracts were compared. As a result, the antioxidant activity was the lowest when the ratio of ethanol and water is 50:50. When antioxidant activity was measured with a 1: 1 mixture of Hwangchil-tree leaf and Hallabong bark extract, it showed higher antioxidant activity than that of Hwangchil-tree leaf extract alone.

Hallabong bark extract has a lower antioxidant activity than Hwangchil tree leaf extract. In the mixed extract of Hwangchil-tree leaf and Hallabong bark extract, the synergistic effect of ABTS radical scavenging activity was found to be 39.73% than that of the Hwangchil-tree leaf extract alone.

Experimental Example 2 Antimicrobial Activity Test against Acne Bacteria of Hwangchil Tree Leaf Extract, Hallabong Bark Extract, and a Mixed Extract of Hwangchil Tree Leaf and Hallabong Bark

Experimental Example 2 was carried out to evaluate the antimicrobial activity effect against acne bacteria against the extracts of Hwangchil-tree leaf extract, Hallabong bark extract and Hwangchil-tree leaf and Hallabong bark extract prepared in Examples 1 and 2 .

Experimental Example 2-1. Antimicrobial Experiment Bacteria and Culture

The strains used in this experiment were Propionibacterium acnes ( P. acnes ) and Malassezia furfur ( M. furfur ), which were distributed by the KCTC (Korean Collection for Type Cultures) at the Microbial Resource Center. Reinforced Clostridial Medium (Table 5) was used as the growth medium for P. acnes , and cultured using Table 6 as the growth medium for M. furfur .

Growth conditions Reinforced Clostridial Medium Temp. (℃) Eptone 10.0 g 37.0 Sodium chloride 5.0 g Beef extract 10.0 g Yeast extract 3.0 g Dexrose 5.0 g Soluble starch 1.0 mL Sodium acetate 3.0 g L-Cysteine hydrochloride 0.5 g Distilled water 1.0 L

Growth conditions Temp. (℃) Eptone 10 g 37.0 Dexrose 20 g Yeast extract 5 g Olive oil 3.3 g Distilled water One L

Experimental Example 2-2. Determination of Antimicrobial Activity

Antimicrobial activity was measured by P. acnes and M. furfur strains. A single colony of the cultured strains was inoculated into the liquid medium, and P. acnes and M. furfur were incubated at 37 ° C. for at least 48 hours, and then 1000 μl of each was uniformly spread on the solid medium. Sterilized disc paper (Toyo seisakusho, 8 mm) was prepared, 50 μl of each sample was injected into the disc, and the flat part was placed on the plated solid medium. The strains placed on the disc were cultured under the conditions of each strain, and after incubation, the clear zone (cm) was checked to determine the antimicrobial activity by the size of the clear zone.

Sample name P. acnes M. furfur Control 50 μl 0.00 0.0 0.5% salicylic acid 50 μl 1.00 - ^* Machi Prefecture Extract 50 μl 0.30 - ^* Yellow Leaf 50% Ethanol Extract 10 μl 1.08 0.0 25 μl 1.38 0.0 50 μl 2.50 0.2 Hallabong Shell Water Extract 10 μl - ^* - ^* 25 μl - ^* - ^* 50 μl 0.0 0.0 Hallabong Shell
50% Ethanol Extract 10 μl - ^* - ^* 25 μl - ^* - ^* 50 μl 0.45 0.2 Hallabong Peel 100% Ethanol Extract 10 μl - ^* - ^* 25 μl - ^* - ^* 50 μl 0.95 0.3 Hwangchil leaves and Hallabong bark 10 μl 1.50 - ^* 25 μl 2.00 - ^* 50 μl 4.00 - ^{* *} -: No experimental data

According to Table 7, Hwangchil-tree leaves were measured with 50% ethanol extract, Hallabong bark water extract, Hallabong bark 50% ethanol extract, Hallabong bark 100% ethanol extract was measured antibacterial activity against acne bacteria. Each sample was inoculated in different amounts to confirm the activity, and each extract was inoculated with 10, 25, 50 μl (or 10, 50 μl) to determine the antimicrobial activity. In addition, the control group was tested with 0.5% salicylic acid (maximum allowable value for washing cosmetics) and gusset extract.

50% ethanol extract of Hwangchil tree showed antimicrobial activity in acne bacteria, and it was found that clear zones of 1.08, 1.38, and 2.50 cm were formed in acne bacteria by inoculation concentrations (10, 25, 50 μl).

Hallabong bark 50% ethanol extract confirmed a clear zone of 0.45 cm at 50 μl inoculation from acne bacteria.

The mixed extract of Hwangchil Tree Leaf Extract and Hallabong Bark Extract forms clear zones of 1.50, 2.00, and 4.00 cm (10, 25, 50 μl) by inoculation concentration in acne bacteria, respectively In view of the larger size than the clear zone, the synergistic effect of the mixed extract was confirmed.

In other words, 4.0 cm clear zone (50 μl inoculation) was formed from P. acnes bacteria, and the mixed extract was better in antibacterial effect than the extract of Hwangchil-tree leaf and Hallabong bark.

Experimental Example 3. Determination of anti-inflammatory effect and NO production inhibitory activity of Hwangchil-tree leaf extract, Hallabong bark extract, and the mixed extract of Hwangchil-tree leaf and Hallabong bark

Experimental Example 3 was prepared in Table (RAW 264.7) to determine the anti-inflammatory activity of the extracts of Hwangchil-tree leaf extract, Hallabong bark extract and Hwangchil-tree leaf and Hallabong bark extract prepared in Examples 1 and 2. To evaluate cell viability.

To measure the anti-inflammatory activity, the toxicity of the cells (RAW 264.7) of the raw material was tested before measuring the NO production inhibitory ability. As a comparative control, 0.5% salicylic acid, which is used as an acne functional cosmetic, and March extract, which is widely used for acne improvement, are used. After diluting 15 times the 50% ethanol extract undiluted solution of Hwangchil-tree leaves, it was prepared in a ratio of 1: 1 with 50% ethanol extract of Hallabong bark and proceeded to make the final concentration of 50% ethanol extract 50% ethanol extract. This sample was considered as a mixed extract stock solution of Hwangchil-tree leaf extract and Hallabong bark extract, and the experiment was carried out by diluting the stock solution, 2 times, 5 times, 10 times, and 20 times.

Experimental Example 3-1. Cell growth inhibition rate of RAW 264.7 cells

Cell survival rate experiments were performed on cells (RAW 264.7) to measure anti-inflammatory activity.

0.5% salicylic acid showed a survival rate of 0.5% to 13.23%, confirming toxicity, and there was no significant difference from the control group after 5-fold dilution. Macula extract showed a significant increase in cell viability compared to the control at all concentrations, which did not inhibit cell viability. 50% ethanol extract of Hilchi leaves did not decrease cell viability in 50%, but rapidly decreased in 5%. Therefore, to confirm the exact cytotoxicity, various diluting folds were prepared to confirm the exact toxicity-inducing concentration. As a result, there was no cytotoxicity in the 30-fold dilution of the Hwangchil-tree leaves, but the toxicity was detected in 20-fold, confirming that the concentration affecting the cell viability was more concentrated than 20-fold. There was no cytotoxicity at all concentrations of the 50% ethanol extract of Hallabong bark, and it was significantly increased in the undiluted and 5-fold dilutions. In addition, the mixture of Hwangchil-tree leaf extract 30 times extract and Hallabong bark extract undiluted solution 1: 1, which had no cell viability inhibitory effect, did not inhibit cell viability in all test solutions. Thus, it was confirmed that the Hwangchil-tree leaf extract had a higher anti-inflammatory effect in the solution of salicylic acid and the solution of salicylic acid than the 20-fold dilution of RAW 264.7 cells causing inflammation.

Sample name RAW 264.7 cell survival rate (%) March extract control (DW) 100.00 ± 1.94 0.5% salicylic acid control (DMSO) 100.00 ± 2.48 March Extract (Comparative Control) Raw material 1000 times diluted 104.54 ± 0.37 100 times diluted raw material 104.67 ± 3.28 10 times dilution of raw material 104.69 ± 2.72 Stock 104.50 ± 1.37 0.5% Salicylic Acid (Comparative Control) 100 times diluted raw material 101.95 ± 5.51 50 times diluted raw material 107.24 ± 2.15 10 times dilution of raw material 106.11 ± 2.80 5 times dilution of raw materials 105.19 ± 2.25 Stock 13.23 ± 0.36 ^** Hwangchil leaves and Hallabong bark control (50% ethanol) 100.00 ± 2.40 Yellow Leaf 50% Ethanol Extract 100 times diluted raw material 96.94 ± 8.10 Raw material 90 times diluted 99.68 ± 3.93 Raw material 80 times diluted 98.00 ± 2.93 Raw material 70 times diluted 98.66 ± 1.48 Raw material 60 times diluted 97.61 ± 1.94 50 times diluted raw material 101.35 ± 2.71 Raw material 40 times diluted 99.05 ± 2.96 Raw material 30 times diluted 97.05 ± 4.57 20 times dilution of raw material 84.78 ± 3.52 ^** 10 times dilution of raw material 54.45 ± 1.21 ^** 5 times dilution of raw materials 11.59 ± 1.04 ^** Stock 11.23 ± 0.72 ^** Hallabong Shell
50% Ethanol Extract 100 times diluted raw material 106.35 ± 4.26 50 times diluted raw material 103.71 ± 1.84 10 times dilution of raw material 105.15 ± 4.27 5 times dilution of raw material 106.71 ± 3.19 ^** Stock 104.97 ± 1.67 ^** Hwangchil leaves and Hallabong bark ^* 20 times dilution of raw material 98.56 ± 2.11 10 times dilution of raw material 100.35 ± 2.18 5 times dilution of raw material 102.53 ± 1.74 2 times dilution of raw materials 102.84 ± 1.67 Stock 102.18 ± 2.74 *: Diluted 15-fold dilutions of Hwangchil leaves, Hallabong bark, and 50% ethanol extract of Hwangchil leaves, and then diluted 1: 1 ratio with 50% ethanol extract of Hallabong. Manufactured to be
**: p <0.05, compared with (-) control.

Experimental Example 3-2. Measurement of inhibition of NO (Nitric Oxide) production in RAW 264.7 cells

Nitric Oxide (NO) production inhibitory activity was measured by diluting Hwangchil-tree leaf extract, Hallabong bark extract, Portulaca oleracea extract, and Salicylic acid extract. In addition, the mixed extract experiment of Hwangchil-tree leaf extract and Hallabong bark extract was prepared at a concentration that does not affect cell viability. 15-fold dilutions of 50% ethanol extract of Hwangchil-tree extract and 50% ethanol extract of Hallabong bark were mixed at a 1: 1 ratio. , 5 times, 10 times, 20 times diluted to proceed with the experiment.

Sample name NO production rate (%) 0.5% salicylic acid control 100.00 ± 4.54 Machi Prefecture Extract Machi Prefecture Control 100.00 ± 1.96 March Extract (Comparative Control) 100 times diluted raw material 130.17 ± 2.28 ^** 10 times dilution of raw material 132.75 ± 0.84 ^** Stock 127.44 ± 2.98 ^** 0.5% Salicylic Acid (Comparative Control) 100 times diluted raw material 95.47 ± 2.52 50 times diluted raw material 94.90 ± 4.85 10 times dilution of raw material 68.64 ± 3.11 ^** 5 times dilution of raw material 50.77 ± 2.83 ^** Hwangchil leaves and Hallabong bark control 100.00 ± 2.45 Yellow Leaf 50% Ethanol Extract 100 times diluted raw material 94.42 ± 4.18 Raw material 90 times diluted 92.35 ± 2.35 ^** Raw material 80 times diluted 94.01 ± 1.35 ^** Raw material 70 times diluted 93.77 ± 2.15 ^** Raw material 60 times diluted 94.38 ± 1.70 ^** 50 times diluted raw material 90.52 ± 1.38 ^** Raw material 40 times diluted 86.33 ± 1.36 ^** Raw material 30 times diluted 82.52 ± 2.48 ^** Hallabong Shell
50% Ethanol Extract 100 times diluted raw material 102.33 ± 2.30 50 times diluted raw material 102.06 ± 0.95 10 times dilution of raw material 101.53 ± 3.06 5 times dilution of raw material 102.94 ± 4.46 Raw stock 113.11 ± 1.52 ^** Hwangchil leaves and Hallabong bark ^* 20 times dilution of raw material 100.70 ± 1.24 10 times dilution of raw material 98.43 ± 3.80 5 times dilution of raw material 95.36 ± 2.37 ^** 2 times dilution of raw materials 84.31 ± 3.01 ^** Raw stock 66.61 ± 2.49 ^** *: Diluted 15-fold dilutions of Hwangchil leaves, Hallabong bark, and 50% ethanol extract of Hwangchil leaves, and then diluted 1: 1 ratio with 50% ethanol extract of Hallabong. Manufactured to be
**: p <0.05, compared with (-) control.

According to Table 9, 50% ethanol extract of Hwangchil-tree leaves confirmed cell viability inhibitory activity up to 20-fold dilution, and 97.05% cell viability at 30-fold dilution did not differ significantly from the control group. In the experiment for measuring NO (Nitric Oxide) production inhibitory activity was carried out at a concentration that does not affect the inhibition of cell viability. In the 30-fold dilutions of 50% ethanol extracts of the yellow lacquer tree, 17.48% showed NO (Nitric Oxide) production inhibition. Hallabong bark 50% ethanol extract showed an increase in NO (Nitric Oxide) in the stock solution. Portion extract from the control group increased NO (Nitric Oxide) at all concentrations, and 0.5% salicylic acid was found to inhibit NO (Nitric Oxide) production of 49.3% in 5-fold dilutions.

The mixed extract of the Hwangchil-tree leaf extract and Hallabong bark extract did not show higher NO (Nitric Oxide) production inhibitory activity than salicylic acid, but the Hwangchil-tree leaf extract had the effect of inhibiting cell viability, and the 30-fold dilution of NO (Nitric Oxide) ) The inhibitory activity was 17.5%, and Hallabong bark extract had no inhibition of NO (Nitric Oxide) production. It was. In other words, Nitric Oxide production inhibition was 33.39%, which was about 16% higher than that of Hilchi leaves. It was confirmed that the anti-inflammatory effect was greatly increased when the Hwangchil-tree leaf extract and Hallabong bark extract were mixed.

Experimental Example 3-3. Inhibition of PG E₂ production in RAW 264.7 cells

Inhibitory activity of Prostaglandin E₂ (PGE₂) was measured to confirm the mechanism of anti-inflammatory activity.

Sample name PGE₂activity (%) Hwangchil Leaf and Hallabong Mixture (-) Control 100.00 ± 12.08 Hwangchil Leaf and Hallabong Mixture 20 times dilution of raw material 100.00 ± 11.97 10 times dilution of raw material 92.15 ± 12.01 ^** 5 times dilution of raw material 67.46 ± 14.40 ^** 2 times dilution of raw materials 30.93 ± 4.58 ^** Stock 23.13 ± 5.57 ^** *: Diluted 15-fold dilutions of Hwangchil leaves, Hallabong bark, and 50% ethanol extract of Hwangchil leaves, and then diluted 1: 1 ratio with 50% ethanol extract of Hallabong. Manufactured to be
**: p <0.05, compared with (-) control.

According to Table 10, PGE₂ production inhibitory activity was confirmed to be significantly reduced compared to the (-) control in the stock, 2-fold, 5-fold, and 10-fold dilutions of the mixed extracts of livewood leaf extract and Hallabong bark extract (PGE₂-lowering ability 76.87 , 69.07, 32.54%), high anti-inflammatory activity of the extract of Hwangchil-tree leaf extract and Hallabong bark extract. In other words, the PGE₂ content of the mixed extract of Hwangchil-tree leaf extract and Hallabong bark extract was inhibited to 23.13% compared to the (-) control at the concentration of the stock solution, indicating high anti-inflammatory activity.

Although the present invention has been described in detail with reference to Examples and Experimental Examples, the present invention is not limited to the above embodiments, and may be modified in various forms, and is commonly used in the art within the technical spirit of the present invention. It is evident that many variations are possible by those of skill in the art. In addition, various forms of substitution, modification, and alteration may be made by those skilled in the art without departing from the technical spirit of the present invention described in the claims, which are also within the scope of the present invention. something to do.

Claims (9)

Cosmetic composition containing Hwangchil-tree leaf extract and Hallabong bark extract as an active ingredient. The method of claim 1,
Cosmetic composition, characterized in that containing 0.1 to 80% by weight of the mixture of the Hwangchil-tree leaf extract and Hallabong bark extract based on the total weight of the cosmetic composition. The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that it has a DPPH radical scavenging ability. The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that it has an ABTS radical scavenging ability. The method of claim 1,
The cosmetic composition is characterized in that it has an antioxidant activity. The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that it has the ability to inhibit the production of nitric oxide (Nitric Oxide). The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that having a prostaglandin (Prostaglandin) E2 production inhibitory ability. The method according to any one of claims 1 to 7,
The cosmetic composition is a cosmetic composition, characterized in that it has a formulation selected from the group consisting of solution, gel, cream, essence, lotion, soap, foam cleansing, mist and serum. The method according to any one of claims 1 to 7,
The cosmetic composition is an anti-acne functional use having antioxidant, anti-inflammatory and antibacterial activity.

Images