CN110604127A - Non-formaldehyde environment-friendly biological specimen preserving fluid - Google Patents
Non-formaldehyde environment-friendly biological specimen preserving fluid Download PDFInfo
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- CN110604127A CN110604127A CN201810617035.8A CN201810617035A CN110604127A CN 110604127 A CN110604127 A CN 110604127A CN 201810617035 A CN201810617035 A CN 201810617035A CN 110604127 A CN110604127 A CN 110604127A
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- solution
- urea
- sodium hydroxide
- buffer solution
- formaldehyde
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
Abstract
The non-formaldehyde environment-friendly biological specimen preservation solution is characterized by comprising plant tannin and a buffer solution with the pH value of 4.0-5.0, wherein the content of tannin in the plant tannin is more than or equal to 8%, the buffer solution is a mixed solution of sodium hydroxide, citric acid, urea, concentrated hydrochloric acid and deionized water, the mass fraction of the plant tannin is 2% -10%, the mass fraction of the sodium hydroxide is 0.5% -1%, the mass fraction of the citric acid is 0.865% -3.5%, the mass fraction of the urea is 0.045% -0.2%, and the volume fraction of the hydrochloric acid is 0.2% -0.48%. The invention can completely replace formalin, and simultaneously solves the problems of pungent smell, volatile active ingredients, poor softness of preserved specimens, poor tissue fixing effect and the like of the traditional preservation solution.
Description
Technical Field
The invention relates to the technical field of preservation of biological specimens, in particular to non-formaldehyde environment-friendly biological specimen preserving fluid.
Background
Formalin is used most widely as a cadaver preservative, formaldehyde and benzene are determined as carcinogens by world health organization, formaldehyde is a recognized carcinogen in the world, is listed as a high-toxicity chemical, can strongly stimulate eyes, skin, respiratory mucosa and the like, finally causes immunologic dysfunction, liver injury and influence on a nerve central system, and can cause diseases such as fetal deformity, leukemia, chronic respiratory diseases, nasopharyngeal carcinoma, female menstrual disorder, acute dysthymia and the like. In addition, the specimen preserved for a long time by the formaldehyde solution can be hardened and yellowed, and simultaneously has white precipitate and turbidity, and the formaldehyde solution can also be reduced in concentration due to the volatilization of the formaldehyde, so that the function of preserving the specimen is gradually lost.
While the harm of the formaldehyde solution for preserving specimens is gradually recognized, some substitute products for the formaldehyde solution, such as benzoic acid-ethanol-potassium nitrate solution, concentrated seawater saturated salt solution, surfactant solution, molinate solution, etc., are also appearing in succession.
The study of the preservation of specimens with benzoic acid-ethanol-potassium nitrate solution began earlier. The benzoic acid has the main function of killing fungi and playing a role in resisting mildew; ethanol can sterilize and dehydrate; the potassium nitrate can improve the permeability, enhance the corrosion resistance and the mildew resistance and has the color retention effect on specimens; in addition, in some damp and hot areas, a small amount of formaldehyde solution is additionally added for achieving better anticorrosion and fixation effects. The multi-component chemicals are matched for use, so that the specimen can be well preserved. The specimen preserved by the preserving fluid has natural color and luster, soft texture, strong hygroscopicity and no mildew phenomenon, the preserving fluid is always clear and transparent, has no precipitate, has no obvious difference with the specimen preserved by the traditional formaldehyde solution, and is an ideal specimen preserving fluid in a short period. However, the preservation solution has many disadvantages. Due to the volatility of ethanol, the preservation solution can be gradually ineffective, and is difficult to popularize especially in hot regions. In addition, the solubility of benzoic acid is low, and the solubility of benzoic acid in 100g of water at 18 ℃ is only 0.27g, so that the mildew-proof effect cannot be achieved by increasing the dosage of benzoic acid. In addition, if the specimen preservation solution is not used together with a small amount of formaldehyde, the fixation effect of the specimen preservation solution cannot meet the requirement of specimen preservation, and the tissue adhesion of the specimen preserved for a long time can occur. Therefore, in the future research, the formulation components of the benzoic acid-ethanol-potassium nitrate solution need to be improved to achieve the ideal effect.
Due to the lack of effective environment-friendly preservative solution, formalin has to be eliminated in countries and regions with high environment-friendly standards, such as the medical colleges of hong Kong university and the medical colleges of some countries in southeast Asia, and human specimens are preserved by a freezing method with high energy consumption.
Therefore, the technicians in the field are dedicated to develop the non-formaldehyde environment-friendly biological specimen preservation solution which can completely replace formalin solution and simultaneously overcome the problems of pungent smell, volatilization of effective components, poor softness of the preserved specimen, poor tissue fixing effect and the like of the traditional preservation solution.
Disclosure of Invention
In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to provide a non-formaldehyde environmental protection biological specimen preservation solution, so as to completely replace formalin solution, and simultaneously overcome the problems of pungent odor, volatilization of effective components, poor softness of preserved specimen, poor tissue fixing effect, etc. of the traditional preservation solution, and simultaneously create a good working environment for medical workers, and provide high quality medical specimen for the medical workers, which will certainly promote better and faster development of medical education and scientific research.
In order to achieve the purpose, the invention provides non-formaldehyde environment-friendly biological specimen preservation solution which is characterized by comprising plant tannin and a buffer solution with the pH value of 4.0-5.0, wherein the content of tannin in the plant tannin is more than or equal to 8%, the buffer solution is a mixed solution of sodium hydroxide, citric acid, urea, hydrochloric acid and deionized water, the mass fraction of the plant tannin is 2% -10%, the mass fraction of sodium hydroxide is 0.5% -1%, the mass fraction of citric acid is 0.865% -3.5%, the mass fraction of urea is 0.045% -0.2%, the volume fraction of hydrochloric acid is 0.2% -0.48%, and the balance is deionized water.
Further, the plant tannin is 1kg, the mass of the buffer solution is 50kg, wherein 374g of sodium hydroxide, 945g of citric acid, 50g of urea, 203mL of hydrochloric acid and the balance of deionized water.
Further, the plant tannin is 2kg, the mass of the buffer solution is 50kg, wherein the buffer solution comprises 296g of sodium hydroxide, 865g of citric acid, 45g of urea, 195mL of hydrochloric acid and the balance of deionized water.
Further, the plant tannin is 5kg, the mass of the buffer solution is 200kg, wherein 595g of sodium hydroxide, 1730g of citric acid, 90g of urea, 350mL of hydrochloric acid and the balance of deionized water are used.
Further, the plant tannin is 10kg, the mass of the buffer solution is 500kg, wherein the buffer solution comprises 1000g of sodium hydroxide, 3500g of citric acid, 200g of urea, 800mL of hydrochloric acid and the balance of deionized water.
Further, the plant tannin is 20kg, the mass of the buffer solution is 1000kg, wherein the buffer solution comprises 2000g of sodium hydroxide, 7000g of citric acid, 400g of urea, 1800mL of hydrochloric acid, and the balance of deionized water.
Further, the preservation solution is colorless transparent liquid, has no pungent smell and contains no volatile substances.
Furthermore, the preservation solution has excellent sterilization and mildew prevention functions and has the effects of anti-thrombus substance formation and thrombus dissolving.
Furthermore, the preservation solution is green and environment-friendly, and the shelf life is more than 10 years.
Compared with the prior art, the non-formaldehyde environment-friendly biological specimen preserving fluid has the following positive effects:
(1) the invention can completely replace formalin solution, and simultaneously overcome the problems of pungent smell, volatile active ingredients, poor softness of preserved specimens, poor tissue fixing effect and the like of the traditional preservation solution;
(2) the non-formaldehyde environment-friendly biological specimen preservation solution disclosed by the invention is a multi-component matching system, can be used for sterilizing and preventing mildew while ensuring that the specimen is properly fixed, and has the advantages of no formaldehyde, no pungent smell, environmental friendliness, greenness, transparent solution, non-volatile effective components, long drug effect, safety, high efficiency and excellent permeability;
(3) the invention solves the problem of formaldehyde which troubles medical workers for more than 100 years, creates a good working environment for the medical workers, provides high-quality medical specimens for the medical workers, and promotes better and faster development of medical education and scientific research.
(4) The non-formaldehyde environment-friendly biological specimen preservation solution is the only domestic environment-friendly product with a fixing effect, fundamentally improves the working teaching environment, improves the quality of preservation and fixation of biological tissue specimens, and generates subversive innovation in the fields of biology, medicine and the like.
Detailed Description
The following describes several preferred embodiments of the present invention to make the technical contents thereof clearer and easier to understand. The present invention may be embodied in many different forms of embodiments and the scope of the invention is not limited to the embodiments set forth herein.
Example 1
Dissolving 1kg of plant tannin with the tannin content of more than or equal to 8% in 50kg of buffer solution with the pH value of 4.0, wherein the buffer solution is prepared by dissolving 374g of sodium hydroxide, 945g of citric acid, 50g of urea and 203ml of hydrochloric acid in deionized water and diluting the mixture to 50kg, and finally obtaining 50kg of environment-friendly specimen preservation solution with the pH value of 4.0.
Example 2
Dissolving 2kg of plant tannin with tannin content more than or equal to 8% in 50kg of buffer solution with pH of 4.5, wherein the buffer solution is prepared by dissolving 296g of sodium hydroxide, 865g of citric acid, 45g of urea and 195ml of hydrochloric acid in deionized water and diluting to 50kg, and finally obtaining 50kg of environment-friendly specimen preservation solution with pH of 4.5.
Example 3
Dissolving 5kg of plant tannin with the tannin content of more than or equal to 8% in 200kg of buffer solution with the pH value of 5.0, wherein the buffer solution is prepared by dissolving 595g of sodium hydroxide, 1730g of citric acid, 90g of urea and 350ml of hydrochloric acid in deionized water to dilute 200kg, and finally obtaining 200kg of environment-friendly specimen preservation solution with the pH value of 4.5.
Example 4
Dissolving 10kg of plant tannin with the tannin content of more than or equal to 8% in 500kg of buffer solution with the pH value of 5.0, wherein the buffer solution is prepared by dissolving and diluting 1000g of sodium hydroxide, 3500g of citric acid, 200g of urea and 800ml of hydrochloric acid with deionized water to 200kg, and finally obtaining 400 kg of environment-friendly specimen preservation solution with the pH value of 4.5.
Example 5
Dissolving 20kg of plant tannin with the tannin content of more than or equal to 8% in 1000kg of buffer solution with the pH value of 5.0, wherein the buffer solution is prepared by dissolving 2000g of sodium hydroxide, 7000g of citric acid, 400g of urea and 1800ml of hydrochloric acid in deionized water and diluting to 200kg, and finally obtaining 800 kg of environment-friendly specimen preservation solution with the pH value of 4.5.
The above 5 embodiments are all developed based on plant tannin to obtain non-formaldehyde environment-friendly biological specimen preservation solution, the main difference lies in the difference in component ratio, the following compares the preservation effect of the specimen of the invention with the existing biological specimen preservation solution on the market to illustrate the benefits of the invention, and the application effect is compared with similar products as shown in the following list:
TABLE 1 preservation effect of display specimen
From the evaluation criteria of preservation effect of the array specimens in table 1, we can conclude by comprehensive analysis: the display specimen preservation effect of the non-formaldehyde environment-friendly biological specimen preservation solution developed based on the plant tannin is optimal, the preservation solution can keep good transparency, the preservation solution cannot generate precipitation, the biological specimen cannot mildew, and the color and luster of the specimen cannot change.
TABLE 2 Effect of preserving teaching samples
From the evaluation criteria of the preservation effect of the teaching specimen in table 2, we can conclude by comprehensive analysis: the non-formaldehyde environment-friendly biological specimen preservation solution developed based on plant tannin has the advantages of optimal effect, no stimulation to cornea, respiratory tract and skin, no harm to human body, no rot, putrefaction and other mildew phenomena of the specimen, and no change of the color of the specimen.
TABLE 3 comparison of the effect of the existing preservative solutions at home and abroad
The comparative analysis of the effect of the existing preserving fluid in China and outside in Table 3 can obtain that: the non-formaldehyde environment-friendly biological specimen preservation solution developed based on the plant tannin has good permeability and fixity, and compared with other preservation solutions, the non-formaldehyde environment-friendly biological specimen preservation solution also has a certain price advantage; the plant tannin is a green and environment-friendly organic phosphine material, has higher antimicrobial property, low toxicity, easy degradation, no residue and no harm to human bodies, so the invention has good environment-friendliness.
The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.
Claims (7)
1. The non-formaldehyde environment-friendly biological specimen preservation solution is characterized by comprising plant tannin and a buffer solution with the pH value of 4.0-5.0, wherein the content of tannin in the plant tannin is more than or equal to 8%, the buffer solution is a mixed solution of sodium hydroxide, citric acid, urea, concentrated hydrochloric acid and deionized water, the mass fraction of the plant tannin is 2% -10%, the mass fraction of the sodium hydroxide is 0.5% -1%, the mass fraction of the citric acid is 0.865% -3.5%, the mass fraction of the urea is 0.045% -0.2%, the volume fraction of the hydrochloric acid is 0.2% -0.48%, and the balance is deionized water.
2. The preservation solution for non-formaldehyde environmental-friendly biological specimens as claimed in claim 1, wherein the mass of the plant tannin is 1kg, and the mass of the buffer solution is 50kg, wherein the solution comprises 374g of sodium hydroxide, 945g of citric acid, 50g of urea, 203mL of hydrochloric acid, and the balance of deionized water.
3. The non-formaldehyde environmental protection biological specimen preservation solution according to claim 1, wherein the plant tannin is 2kg, the buffer solution has a mass of 50kg, and the solution comprises 296g of sodium hydroxide, 865g of citric acid, 45g of urea, 195mL of hydrochloric acid, and the balance of deionized water.
4. The preservation solution for the non-formaldehyde environmental-friendly biological specimen as claimed in claim 1, wherein the plant tannin is 5kg, the mass of the buffer solution is 200kg, and the preservation solution comprises 595g of sodium hydroxide, 1730g of citric acid, 90g of urea, 350mL of hydrochloric acid, and the balance of deionized water.
5. The non-formaldehyde environmental protection biological specimen preservation solution according to claim 1, wherein the plant tannin is 10kg, the buffer solution has a mass of 500kg, and comprises 1000g of sodium hydroxide, 3500g of citric acid, 200g of urea, 800mL of hydrochloric acid, and the balance of deionized water.
6. The non-formaldehyde environmental protection biological specimen preservation solution according to claim 1, wherein the plant tannin is 20kg, the buffer solution has a mass of 1000kg, wherein the buffer solution comprises 2000g of sodium hydroxide, 7000g of citric acid, 400g of urea, 1800mL of hydrochloric acid, and the balance of deionized water.
7. The non-formaldehyde environmental-friendly biological specimen preservation solution according to claim 1, wherein the preservation solution is a colorless transparent liquid, has no pungent smell, and contains no volatile substances.
Priority Applications (1)
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| CN201810617035.8A CN110604127A (en) | 2018-06-14 | 2018-06-14 | Non-formaldehyde environment-friendly biological specimen preserving fluid |
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| CN201810617035.8A CN110604127A (en) | 2018-06-14 | 2018-06-14 | Non-formaldehyde environment-friendly biological specimen preserving fluid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113519505A (en) * | 2021-09-01 | 2021-10-22 | 宁波西敦医药包衣科技有限公司 | Environment-friendly water-based cross-linking agent biological tissue preservation material and preparation method thereof |
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