CN106719598A - A kind of composition and the environmentally friendly specimen preserving liquid prepared with said composition - Google Patents
A kind of composition and the environmentally friendly specimen preserving liquid prepared with said composition Download PDFInfo
- Publication number
- CN106719598A CN106719598A CN201611067326.1A CN201611067326A CN106719598A CN 106719598 A CN106719598 A CN 106719598A CN 201611067326 A CN201611067326 A CN 201611067326A CN 106719598 A CN106719598 A CN 106719598A
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- composition
- animal
- corpse
- specimen
- environmentally friendly
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Abstract
The environmentally friendly specimen preserving liquid with controllable fixation prepared the invention discloses a kind of composition preserved for corpse and animal or plant specimen and with said composition, is related to the anti-corrosion of corpse and animal or plant specimen in medical teaching and research work, preservation, mould proof etc..Said composition includes anticorrosive mildewproof active component, co-penetrant, controllable secured adjusted agent and solvent.The environmentally friendly specimen preserving liquid is that the composition is dissolved in solvent to be prepared from.The anticorrosive mildewproof active component is a Plant Extracts Geniposide.The present invention is that its own has certain fixation compared with other existing specimen preserving liquids, can be used for the anti-corrosion of corpse and animal or plant specimen, preservation, mould proof etc..The characteristics of preservation liquid is that the anticorrosive mildewproof time is long, effect is good, and without formaldehyde, safety non-toxic, active ingredient is non-volatile, and cost of material is easy to get, cheap.
Description
Technical field
The present invention relates to anti-corrosion, preservation, the Prevention of Mould Development of corpse and animal or plant specimen in medical teaching and research work
Field, more particularly to a kind of composition and the environmentally friendly Saving specimen with controllable fixation prepared with said composition
Liquid.
Background technology
In medical science, biological analysis, observation, research process, animal and tissue are all first to fix, and reprocess, see
Examine, detect, preserve.The fixation of animal and tissue is, by a series for the treatment of such as fixative, dehydrating agent, to keep cell, group
The intrinsic form and structure knitted, reaching prevents bacterial corrosion and tissue automatic soup-dissolving, preserves the intrinsic material of cell, is easy to the hardening of tissue
Stripping and slicing, prevent disease's spread and enhancing dyeing etc. purpose.The most frequently used histologic fixatives are formalin, and it has fixed equal
Even, penetration power is strong, preserve lipid material, low cost and other advantages.But its impurity content is more, influence after the sour change of fixer dyeing,
The shortcomings of uric acid and glucide, readily volatilized, pollution environment, dry sample, mildew resistance difference can not be fixed also imply that it must
Will move out the fixed stage of tissue.
In order to reduce harm of the formaldehyde to human body and environment, scientific research personnel have developed material and the side of various replacement formaldehyde
Method, such as phenols solution, salt solution, bromogeramine solution, heavy metal compound etc..However, phenols solution is to peripheral nerve
Tip has detrimental effect, and phenol oxidation is variable after long-time, to there is yellowish-brown mud sediment thing into benzoquinones in corpse pond, can reduce anti-corrosion
Effect.Salt solution often with 20% sodium chloride solution, because concentration dehydration high is too strong, cadaveric tissue can be received because of dehydration
Contracting, certain difficulty is brought to dissection.A kind of bactericide of bromogeramine solution, typical concentrations are 0.1-0.2%, nonirritant, poison
Property it is low, have a stronger sterilizing power, but because bromogeramine easily by organic substance influence reducing effect, many inorganic salts adapteds are prohibited
Avoid, and should avoid using aluminum apparatus.Because there is severe toxicity, its application is also very restricted heavy metal salt.At present on the market
Still certain pollution can not be caused to environment while Saving specimen effect is reached without a kind of environmentally friendly specimen preserving liquid.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of composition preserved for corpse and its animal or plant specimen,
Said composition is preserved using a Plant Extracts Geniposide as anticorrosive mildewproof active component for corpse and its animal or plant specimen
Embalming, mould proof, preservation problem can be efficiently solved, while Geniposide also has certain fixation.The combination
The characteristics of thing, is free from formaldehyde, and safety non-toxic, active ingredient is non-volatile, and cost of material is easy to get, cheap.
In order to solve the above-mentioned technical problem, the present invention is adopted the technical scheme that：
A kind of composition preserved for corpse and its animal or plant specimen, said composition include anticorrosive mildewproof active component,
Co-penetrant and controllable secured adjusted agent, it is characterised in that the anticorrosive mildewproof active component is a Plant Extracts capital Buddhist nun
It is flat, the co-penetrant be in nitrate, sulfate or hydrochloride one or two and above with arbitrary proportion mix it is mixed
Compound, the controllable secured adjusted agent is the small organic molecules such as urea.
Each component weight portion is：
Geniposide：0.5-5 weight portions
Co-penetrant：0.5-5 weight portions
Controllable secured adjusted agent：0.5-2 weight portions
Wherein, the co-penetrant be potassium nitrate, calcium nitrate, sodium nitrate, magnesium nitrate, potassium sulfate, calcium sulfate, sodium sulphate,
The mixture of one or more arbitrary proportions mixing in magnesium sulfate, potassium chloride, calcium chloride, sodium chloride, magnesium chloride.It is described
Controllable secured adjusted agent is the small organic molecules such as urea.
The preparation method of the composition comprises the following steps：
A. Geniposide 0.5-5 weight portions are weighed, co-penetrant 0.5-5 weight portions are added, is stirred at normal temperatures and pressures；
B. the mixture in step A adds controllable secured adjusted agent 0.5-2 weight portions, stirs at normal temperatures and pressures.
A kind of environmentally friendly specimen preserving liquid, is that will be previously used for the composition that corpse and its animal or plant specimen preserve to be dissolved in solvent
In be prepared from, the solvent is the common solvents such as water, methyl alcohol, ethanol.
The of the invention beneficial effect to be reached is：
1. the present invention applies in embalming preservation a Plant Extracts Geniposide, meanwhile, with certain proportion plus
Enter the small organic molecules such as co-penetrant and urea, a kind of environmental protection mark with fixation is made after being dissolved in water, methyl alcohol, ethanol
This preservation liquid, the various moulds having to corpse and animal or plant specimen are effectively killed and suppress, while accomplishing to the controllable of sample
It is fixed.
2. environmentally friendly specimen preserving liquid of the invention is free of formaldehyde, and safety non-toxic, active ingredient is non-volatile, to human body and environment
Harmless, experiment proves that corpse and animal or plant specimen do not go mouldy after soaking, do not shrink, color is natural, it is flexible to organize, moistening,
Using effect is good.
3. environmentally friendly Saving specimen liquid and preparation method thereof of the invention is simple, and cost of material is easy to get, cheap, and being easy to promote should
With.
Brief description of the drawings
Fig. 1 (a)-Fig. 1 (d) is histopathologic slide's photo 1；
Fig. 2 (a)-Fig. 2 (d) is histopathologic slide's photo 2；
Fig. 3 (a)-Fig. 3 (d) fresh animal tissue is fixed and preserves photo；
Wherein,
Fig. 1 (a) is fat cell histopathologic slide, and Fig. 1 (b) is ileum histopathologic slide, and Fig. 1 (c) is spinal nerve
Section histopathologic slide, Fig. 1 (d) is medulla of lymph gland histopathologic slide；
Fig. 2 (a) is loose connective tissue histopathologic slide, and Fig. 2 (b) is hyaline cartilage histopathologic slide, Fig. 2 (c)
It is mixed thrombus histopathologic slide, Fig. 2 (d) is enteraden cancer histopathologic slide；
Fig. 3 (a) is to fix the liver for preserving through environmentally friendly specimen preserving liquid, and Fig. 3 (b) is to fix to protect through environmentally friendly specimen preserving liquid
The kidney deposited, Fig. 3 (c) is to fix the heart for preserving through environmentally friendly specimen preserving liquid, and Fig. 3 (d) is to be fixed through environmentally friendly specimen preserving liquid
The musculature of preservation.
Specific embodiment
Embodiment 1
A kind of composition preserved for corpse and its animal or plant specimen, it is made up of Geniposide, calcium nitrate and urea,
The weight of said composition is constituted：
Geniposide：40g
Calcium nitrate：20g
Urea：10g
Preparation method of composition is：Geniposide 40g is weighed, calcium nitrate 20g is added, stirred at normal temperatures and pressures；Again
Urea 10g is added in said mixture, is stirred at normal temperatures and pressures.
Said composition is dissolved in deionized water to 1000g, environmentally friendly specimen preserving liquid is prepared.
Normal temperature and pressure is stirred, and finished product is after standing.
Embodiment 2
A kind of composition preserved for corpse and its animal or plant specimen, it is made up of Geniposide, calcium nitrate and urea,
The weight of said composition is constituted：
Geniposide：50g
Sodium nitrate：30g
Urea：15g
Preparation method of composition is：Geniposide 50g is weighed, calcium nitrate 30g is added, stirred at normal temperatures and pressures；Again
Urea 15g is added in said mixture, is stirred at normal temperatures and pressures.
Said composition is dissolved in deionized water to 1000g, environmentally friendly specimen preserving liquid is prepared.
Normal temperature and pressure is stirred, and finished product is after standing.
Embodiment 3
A kind of composition preserved for corpse and its animal or plant specimen, it is made up of Geniposide, calcium nitrate and urea,
The weight of said composition is constituted：
Geniposide：40g
Sodium chloride：20g
Urea：15g
Preparation method of composition is：Geniposide 40g is weighed, calcium nitrate 20g is added, stirred at normal temperatures and pressures；Again
Urea 15g is added in said mixture, is stirred at normal temperatures and pressures.
Said composition is dissolved in deionized water to 1000g, environmentally friendly specimen preserving liquid is prepared.
Normal temperature and pressure is stirred, and finished product is after standing.
Embodiment 4
A kind of composition preserved for corpse and its animal or plant specimen, it is made up of Geniposide, calcium nitrate and urea,
The weight of said composition is constituted：
Geniposide：40g
Calcium nitrate：20g
Urea：10g
Preparation method of composition is：Geniposide 40g is weighed, calcium nitrate 20g is added, stirred at normal temperatures and pressures；Again
Urea 10g is added in said mixture, is stirred at normal temperatures and pressures.
Said composition is dissolved in methyl alcohol to 1000g, environmentally friendly specimen preserving liquid is prepared.
Normal temperature and pressure is stirred, and finished product is after standing.
Embodiment 5
A kind of composition preserved for corpse and its animal or plant specimen, it is made up of Geniposide, calcium nitrate and urea,
The weight of said composition is constituted：
Geniposide：40g
Calcium nitrate：20g
Urea：10g
Preparation method of composition is：Geniposide 40g is weighed, calcium nitrate 20g is added, stirred at normal temperatures and pressures；Again
Urea 10g is added in said mixture, is stirred at normal temperatures and pressures.
Said composition is dissolved in ethanol to 1000g, environmentally friendly specimen preserving liquid is prepared.
Normal temperature and pressure is stirred, and finished product is after standing.
Experimental example 1
Environmentally friendly specimen preserving liquid is preserved to the fixed of pathological tissue, and the preservation liquid for being used is prepared by embodiment 1.
This preservation liquid is applied into the fixed of the pathological tissues such as fat cell, intestines, neuromere, lymph node to preserve.Fig. 1 (a)-
Fig. 1 (d) is that, through the histopathologic slide's photo after this environmentally friendly specimen preserving liquid, its cellular morphology, background distinguish all obvious,
Can the preferable original construction of response organization's cell, contribute to pathological analysis.As can be seen here, this environmentally friendly specimen preserving liquid is for pathology
The fixed preservation effect of tissue is excellent.
Experimental example 2
Environmentally friendly specimen preserving liquid is preserved to the fixed of pathological tissue, and the preservation liquid for being used is made by embodiment 4.
This preservation liquid is applied to the fixation of the pathological tissues such as loose connective tissue, hyaline cartilage, mixed thrombus, enteraden cancer
Preserve.Fig. 2 (a)-Fig. 2 (d) is through the histopathologic slide's photo after this environmentally friendly specimen preserving liquid, its cellular morphology, background area
It is point all obvious, can the preferable original construction of response organization's cell, contribute to pathological analysis.As can be seen here, this environmentally friendly sample
Preserve liquid excellent for the fixed preservation effect of pathological tissue.
Experimental example 3
Environmentally friendly specimen preserving liquid is preserved to the fixed of fresh animal tissue, is made by embodiment 5.
This preservation liquid is applied into fresh animal liver, kidney, heart, the fixed of musculature to preserve.Soaked guarantor
Deposit with this Project Product, by the observation of a year, see whether tissue occurs the phenomenons such as corruption, contraction, self-dissolving.Fig. 3 (a)-Fig. 3
D () is pig liver, kidney, heart, the musculature of preservation 1 year, it is seen that this environment-friendly type specimen preserving liquid can be preserved preferably
Fresh animal tissue, and preservation liquid is still as clear as crystal by the long period, there is no substantially contraction in animal tissue, form is preserved
It is intact.
Claims (5)
1. a kind of composition preserved for corpse and animal or plant specimen, said composition includes anticorrosive mildewproof active component, helps and ooze
Saturating agent and controllable secured adjusted agent, it is characterised in that the anticorrosive mildewproof active component is a Plant Extracts Geniposide,
The co-penetrant is one or more mixtures mixed with arbitrary proportion in nitrate, sulfate or hydrochloride；
The each component weight portion of the composition is：
Geniposide：0.5-5 weight portions
Co-penetrant：0.5-5 weight portions
Controllable secured adjusted agent：0.5-2 weight portions.
2. a kind of composition preserved for corpse and animal or plant specimen according to claim 1, it is characterised in that preferably
, the co-penetrant is potassium nitrate, calcium nitrate, sodium nitrate, magnesium nitrate, potassium sulfate, calcium sulfate, sodium sulphate, magnesium sulfate, chlorination
One or more mixtures mixed with arbitrary proportion in potassium, calcium chloride, sodium chloride, magnesium chloride.
3. a kind of composition preserved for corpse and animal or plant specimen according to claim 1, it is characterised in that described
Controllable secured adjusted agent is small organic molecule.
4. a kind of composition preserved for corpse and animal or plant specimen according to claim 1, it is characterised in that described
Controllable secured adjusted agent is urea.
5. a kind of environmentally friendly specimen preserving liquid preserved for corpse and animal or plant specimen, it is as described in claim any one of 1-4
Composition be dissolved in solvent in be prepared from, the solvent is the common solvents such as water, methyl alcohol, ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611067326.1A CN106719598B (en) | 2016-11-28 | 2016-11-28 | Composition and environment-friendly specimen preservation solution prepared from same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611067326.1A CN106719598B (en) | 2016-11-28 | 2016-11-28 | Composition and environment-friendly specimen preservation solution prepared from same |
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| Publication Number | Publication Date |
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| CN106719598A true CN106719598A (en) | 2017-05-31 |
| CN106719598B CN106719598B (en) | 2020-07-24 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094410A (en) * | 2018-01-26 | 2018-06-01 | 山东大学齐鲁医院 | A kind of skin profound hypothermia protective agent and vitrification method |
| CN109042622A (en) * | 2018-09-28 | 2018-12-21 | 河南科技大学 | A kind of guarantor's color fixing agent and preparation method thereof and the application in prepared by sample |
| CN110604127A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly biological specimen preserving fluid |
| CN110604126A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly pathological tissue fixing agent |
| CN111226905A (en) * | 2020-03-25 | 2020-06-05 | 张家港市德仁科教仪器设备有限公司 | Novel environment-friendly specimen fixing composition, preparation method thereof and fixing liquid containing composition |
| CN113439741A (en) * | 2021-08-05 | 2021-09-28 | 湖北省农业科学院中药材研究所 | Preparation method of primary color soaked specimen of snake mushroom |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094410A (en) * | 2018-01-26 | 2018-06-01 | 山东大学齐鲁医院 | A kind of skin profound hypothermia protective agent and vitrification method |
| CN108094410B (en) * | 2018-01-26 | 2021-08-31 | 山东大学齐鲁医院 | Skin deep hypothermia protective agent and skin preservation method |
| CN110604127A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly biological specimen preserving fluid |
| CN110604126A (en) * | 2018-06-14 | 2019-12-24 | 宁波化奇工程技术有限公司 | Non-formaldehyde environment-friendly pathological tissue fixing agent |
| CN109042622A (en) * | 2018-09-28 | 2018-12-21 | 河南科技大学 | A kind of guarantor's color fixing agent and preparation method thereof and the application in prepared by sample |
| CN111226905A (en) * | 2020-03-25 | 2020-06-05 | 张家港市德仁科教仪器设备有限公司 | Novel environment-friendly specimen fixing composition, preparation method thereof and fixing liquid containing composition |
| CN113439741A (en) * | 2021-08-05 | 2021-09-28 | 湖北省农业科学院中药材研究所 | Preparation method of primary color soaked specimen of snake mushroom |
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| CN106719598B (en) | 2020-07-24 |
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