CN101438698B - Anti-corrosive perfusate and preservative fluid of biological wet carcase - Google Patents
Anti-corrosive perfusate and preservative fluid of biological wet carcase Download PDFInfo
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- CN101438698B CN101438698B CN2008101439805A CN200810143980A CN101438698B CN 101438698 B CN101438698 B CN 101438698B CN 2008101439805 A CN2008101439805 A CN 2008101439805A CN 200810143980 A CN200810143980 A CN 200810143980A CN 101438698 B CN101438698 B CN 101438698B
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Abstract
The invention discloses a preservative infusion fluid and a preservative fluid for biological wet dead bodies. The preservative infusion fluid comprises the following components according to weight percentage: 4 percent to 12 percent of methanal, 6 percent to 20 percent of absolute ethyl alcohol, 0.1 percent to 1.5 percent of glycerin, 0.9 percent of sodium chloride, 1 percent to 2.5 percent of ferric chloride, 1 percent to 2.5 percent of zinc chloride, 1 percent to 2.5 percent of aluminium chloride, 0.2 percent to 1.2 percent of thymol, 1 percent to 2.5 percent of urotropine and the rest, i.e., distilled water. Compared with the preservative fluid of the prior art, the preservative infusion fluid and the preservative fluid effectively solve the problem of preservation of the cultural relics of the biological wet dead bodies, due to the adoption of the invention, the biological wet dead bodies can be prevented form being rotten, mildewed, discolored, dehydrated, decalcified and degraded in protein, the natural form of the unearthed cultural relics can be well maintained, and the invention has the advantages of high safety coefficient, stable performance, good repetitiveness, and the like.
Description
Technical field
The present invention relates to the biological antiseptic technical field, relate in particular to a kind of preservative of historical relic class biological wet carcase.
Background technology
After the histocyte of animal or human's remains lost vigor, organelle disintegrations such as lysosome were decomposed in the effect undertissue of various enzymes, and various organs are softening, liquefaction, remains autolyze; Again because the digestive juice in the remains digestive organs produces autopepsia, adding anoxic, bacterium and toxin thereof etc. all quickens lysosome membrane and breaks, hydrolase in the lysosome discharges in a large number and destroys cell, break-up tissue, produces the vigor that inoxidizable product has increased hydrolase again.Under the influence of the putrefying bacteria inside and outside the remains, parasite and external environment condition, dead body tissue accelerated decomposition, remains corruption that Here it is.Preventing to continue corruption after the wet corpse of historical relic is unearthed, is the key that historical relic is preserved.
At present the world of medicine is existing multiple is used for the preservative that animal or human's class embalming perfusion is handled or preserved, and as the most commonly used formalin (10～40% formaldehyde) solution is arranged, and usually is used for perfusion and preserve cadaver sample.In addition, also just like 2～3% phenols, bromogeramine solution or the like.Chinese patent CN1047061C reported a kind of antiseptic preserving fluid of being made up of benzoate, ethanol, potassium nitrate or sodium nitrate, glycerine.CN1081814A has reported and a kind ofly has been dissolved in formaldehyde and alcoholic solvent and a kind of preservative that is used to soak animal or plant specimen of making by boric acid, sodium chloride and phenol.But have disclosed these embalming liquid of document now, all be not suitable for the biological wet carcase of historical relic class.The biological wet carcase of historical relic class is underground seals the wet corpse of historical relic that is unearthed in back for many years up for safekeeping, because dead of the remote past, and the human body structure of corpse and the just very big difference of existence between the dead organisms.Compare fresh wet corpse, the wet corpse body tissue of historical relic is very fragile, to impregnating fluid anticorrosion, protect many-sided performances such as look, anti-dehydration decalcification and require to exceed a lot far away.
Because the particularity and the value of mummy historical relic, the method that adopts existing impregnating fluid to pour into and preserve corpse far can not satisfy the preservation requirement of mummy historical relic, be difficult to make the corpse of preservation reach anticorrosion, the bacterium of going out, softening, keep purposes such as color is constant.Therefore; how preventing that its variable color, dehydration, decalcification and protein degradation are to keep its original appearance when unearthed; and prolong the holding time as much as possible, and be the whole technical bottleneck of preserving of the wet corpse type historical relic of restriction always, also be the emphasis and the difficult point of wet corpse class historical relic's protection work.
Summary of the invention
The objective of the invention is to solve the problem that the used biological antiseptic liquid of prior art can not be applicable to wet corpse historical relic, a kind of anti-corrosive perfusate that wet corpse type historical relic perfusion is handled that is used for is provided, with the wet corpse of this anti-corrosive perfusate perfusion, can prevent effectively that the wet corpse historical relic of biological species is rotten, mouldy, variable color, dehydration, decalcification and protein degradation, keep the original appearance when unearthed in long-time.
Another object of the present invention also is to provide a kind of antiseptic preserving fluid that is used to soak wet corpse type historical relic, thereby realizes the stable long preservation effectively to biological wet carcase class unearthed relics.
Detailed technology scheme of the present invention is:
A kind of anti-corrosive perfusate of biological wet carcase, form by following composition by weight percentage: formaldehyde 4～12%, absolute ethyl alcohol 6～20%, glycerine 0.1%～1.5%, sodium chloride 0.9%, ferric trichloride, zinc chloride, aluminium chloride trisection 1%～2.5%, thymol 0.2～1.2%, the distilled water of methenamine 1～2.5% and surplus.Absolute ethyl alcohol is meant that the ethanol mass concentration is not less than 99.5%.
The compound method of above-mentioned anti-corrosive perfusate is: under the normal temperature and pressure, get formaldehyde, absolute ethyl alcohol and glycerine by above-mentioned prescription and add in the distilled water, fully stir; Ferric trichloride, zinc chloride and the aluminium chloride of getting five equilibrium by prescription add fully dissolving in the distilled water; Methenamine, thymol, the sodium chloride of formula ratio are dissolved in distilled water, three part solution are mixed, fully stir and promptly get anti-corrosive perfusate of the present invention.
The said antiseptic preserving fluid that is used for biological wet carcase among the present invention, form by following composition by weight percentage: formaldehyde 3.5%～4%, absolute ethyl alcohol 12～18%, glycerine 0.1%～1.5%, ferric trichloride, zinc chloride, aluminium chloride trisection 1%～2.5%, methenamine 1～2.5%, the distilled water of sodium chloride 0.9 ± 0.05% and surplus.
The preparation method of antiseptic preserving fluid is: get formaldehyde, absolute ethyl alcohol and glycerine by proportioning and add in the distilled water, fully stir; Ferric trichloride, zinc chloride, the aluminium chloride of getting five equilibrium by proportioning add fully dissolving in the distilled water; With formula ratio methenamine and sodium chloride be dissolved in distilled water; Three part solution are mixed, fully stir and promptly get antiseptic preserving fluid of the present invention.
In the use, earlier the wet corpse of unearthed relics is carried out whole body and the perfusion of inner internal organs, anti-corrosive perfusate is evenly penetrated into wet the each several part tissue of corpse, and then soak preservation with antiseptic preserving fluid of the present invention with anti-corrosive perfusate of the present invention.The anticorrosion perfusion that perfusion liquid is used for after wet corpse is unearthed is handled, and promptly the biological organs of wet corpse is anticorrosion; The whole liquid that preservation liquid is used for wet corpse soaks, and promptly the peripheral environment of wet corpse is anticorrosion.Perfusion liquid is identical with the two most of component of preservation liquid, but according to the different requirements of pouring into and preserving, fills a prescription flexibly, and work is suitable on some component and ratio adjusts.
Impregnating fluid compared to existing technology, formaldehyde in anti-corrosive perfusate of the present invention and the antiseptic preserving fluid prescription, ethanol, slaine can make protein coagulating rapidly, stop the decomposition of cathepsin to suppress the growth and breeding of bacterium, thereby prevented corrupt generation.Add methenamine in the impregnating fluid and have following effect: the one, disinfection, the 2nd, in and acidic materials, the 3rd, react as crosslinking agent and combined with protein, it is lost activity.Add thymol in the perfusion liquid and can prevent that wet corpse from going mouldy.Add glycerine and can prevent wet corpse dehydration, promptly keep wet corpse moisture state.The present invention efficiently solves the difficult problem that the wet corpse historical relic of biological species is preserved, adopt that the present invention can prevent effectively that the wet corpse historical relic of biological species is rotten, mouldy, variable color, dehydration, decalcification and protein degradation, and well keep original appearance when being unearthed, have advantages such as safety coefficient height, stable performance, good reproducibility.
Description of drawings
Fig. 1: the hepatic tissue after two kinds of anti-corrosion stationary liquids are fixing and the om observation of skeletal muscle (HE dyeing, * 400)
A: impregnating fluid A
1Liver after fixing; B: the liver after formalin solution C is fixing
C: impregnating fluid A
1Skeletal muscle after fixing; D: the skeletal muscle after formalin solution C is fixing
Embodiment
Embodiment 1: preparation anti-corrosive perfusate A
1
Component and quality proportioning: formaldehyde 4.8%, absolute ethyl alcohol 14%, glycerine 1%, sodium chloride 0.9%, heavy metallic salt ferric trichloride, zinc chloride, aluminium chloride trisection totally 1.5%, thymol 0.8%, the distilled water of methenamine 2% and surplus.Measure formaldehyde, absolute ethyl alcohol, glycerine by said ratio, add in the distilled water and fully stir; Ferric trichloride, zinc chloride, the aluminium chloride of getting five equilibrium add abundant stirring and dissolving in the distilled water; Get methenamine, thymol, sodium chloride and be dissolved in distilled water, three part solution are mixed, fully stir and promptly get anti-corrosive perfusate.
Embodiment 2: preparation anti-corrosive perfusate A
2
Component and quality proportioning: formaldehyde 11.8%, absolute ethyl alcohol 12%, glycerine 1%, sodium chloride 0.9%, heavy metallic salt ferric trichloride, zinc chloride, aluminium chloride trisection totally 1%, thymol 0.8%, the distilled water of methenamine 2% and surplus.Measure formaldehyde, absolute ethyl alcohol, glycerine by said ratio, add in the distilled water and fully stir; Ferric trichloride, zinc chloride, the aluminium chloride of getting five equilibrium add abundant stirring and dissolving in the distilled water; Get methenamine, thymol, sodium chloride and be dissolved in distilled water, three part solution are mixed, fully stir and promptly get anti-corrosive perfusate.
Embodiment 3: preparation antiseptic preserving fluid B
1
Component and quality proportioning: formaldehyde 3.8%, absolute ethyl alcohol 15%, glycerine 1%, ferric trichloride, zinc chloride, aluminium chloride trisection totally 2%, methenamine 1.5%, the distilled water of sodium chloride 0.95% and surplus.Measure formaldehyde, absolute ethyl alcohol, glycerine by said ratio, add in the distilled water and fully stir; Ferric trichloride, zinc chloride, the aluminium chloride of getting five equilibrium add abundant stirring and dissolving in the distilled water; Get methenamine, thymol, sodium chloride and be dissolved in distilled water, three part solution are mixed, fully stir and promptly get antiseptic preserving fluid.
Application Example
The one tool corpse that wets in somewhere is immersed in when unearthed in the coffin liquid, and its profile is complete, and internal organ are various, and human body is plentiful, and is still flexible, and extremities joint still can be movable; When dissected, muscular tissue is well arranged, and nervus vasculairs are excellent; Microscopy undertissue structure is preserved also better, can also find cell structure in some tissue.Handle preservation with the inventive method after being unearthed, concrete steps are:
Get perfusion liquid A
1, the perfusion flow velocity is: 5 μ l/s, injection pressure are 80mmHg.Take loci thoracic cavity after-teeming perfusion liquid: select the bilateral linea medioclavicularis, from the puncture after-teeming of the 2nd, the 5th intercostal space; Select infrascapular line, from the puncture after-teeming of the 8th intercostal space, the entry needle paracentesis depth is as the criterion to break through parietal pleura.Multidigit point abdominal cavity after-teeming perfusion liquid then: select the center puncture after-teeming perfusion liquid in hypochondriac region, the left and right sides, left right iliac region, umbilical zone, the entry needle paracentesis depth is as the criterion to break through parietal peritoneum.Oral cavity, nasal cavity, middle ear perfusion perfusion liquid: the direct cannulation method is injected perfusion liquid.The total amount of described perfusion liquid for wet corpse heavy 4.5%.To move in the special preservation case by wet corpse through the historical relic that aforesaid operations is handled well, and fill it up with and preserve liquid B
1, airtight preservation.
The pharmacodynamics Comparative Examples
The inventor uses formalin (20% formaldehyde) solution (C liquid) and anti-corrosive perfusate (A of the present invention respectively
1Liquid), respectively two kinds of impregnating fluids are carried out the physicochemical property experiment of drug susceptibility experiment, anticorrosion sample and the histology morphological observation of sample.
1. drug susceptibility experiment
Adopt aseptic cotton examination to give and Candida albicans, staphylococcus aureus, surperficial staphylococcus, streptococcus are evenly coated on the nutrient agar panel every kind of various 2 flat boards of bacterium respectively.In each agar plate central authorities, get the about 7mm of a diameter, dark 3mm, circular hole, with impregnating fluid A
1Be added in respectively in the circular hole with 20% formalin, every hole 25ul cultivates observed result after 24 hours down, with its antibacterial ring of kind of calliper, the results are shown in Table 1 for 35 ℃.Experiment shows, A
1Fungi is had inhibitory action, and C is to fungi unrestraint effect.
The experiment of table 1 drug susceptibility
Use antiseptic preserving fluid B
1Soak sample 6 years, accumulative total is preserved sample 100 tools, and corpse is not seen rotten and fungus growth.
2. tectology relatively
Adopt antiseptic preserving fluid B
1And formalin (20% formaldehyde) solution (C liquid) fix bone muscular tissue and hepatic tissue, after 5 years, under light microscopic, observe the variation of the structure of its tissue morphology, compare research, the results are shown in accompanying drawing 1 and table 2.
Table 2 light microscopic undertissue structure comparing result
3. the physicochemical property of the anticorrosion sample of sample experiment
Use A respectively
2Anti-corrosive perfusate and B
1Antiseptic preserving fluid (be called for short newly prescription), by fresh adult's corpse 50 tools of above-mentioned application examples step process, and in contrast with 20% formalin (abbreviation conventional formulation), adopt same step process corpse 50 tools, after 5 years, color, corpse pliability, organ structure to the sample of anticorrosion preservation are assessed, and the results are shown in Table 3.
Table 3 is preserved liquid to the sample preservation effect relatively for two kinds
Claims (3)
1. the anti-corrosive perfusate of a biological wet carcase is made up of following composition: formaldehyde 4.8%, absolute ethyl alcohol 14% by weight percentage, glycerine 1%, sodium chloride 0.9%, ferric trichloride, zinc chloride, aluminium chloride trisection totally 1.5%, thymol 0.8%, the distilled water of methenamine 2% and surplus.
2. the anti-corrosive perfusate of a biological wet carcase is made up of following composition: formaldehyde 11.8%, absolute ethyl alcohol 12% by weight percentage, glycerine 1%, sodium chloride 0.9%, ferric trichloride, zinc chloride, aluminium chloride trisection totally 1%, thymol 0.8%, the distilled water of methenamine 2% and surplus.
3. the antiseptic preserving fluid of a biological wet carcase is made up of following composition: formaldehyde 3.8%, absolute ethyl alcohol 15% by weight percentage, glycerine 1%, ferric trichloride, zinc chloride, aluminium chloride trisection totally 2%, methenamine 1.5%, the distilled water of sodium chloride 0.95% and surplus.
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| CN101438698B true CN101438698B (en) | 2011-11-30 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010303A (en) * | 2015-07-10 | 2015-11-04 | 毕爱胜 | Corruption remains restoring method |
| ES2725301B2 (en) * | 2018-03-23 | 2020-05-27 | Rodino Ramon Angel Soto | EXITUS CONSERVATION COMPOSITION, ITS MANUFACTURE AND ITS USE |
| CN109452261A (en) * | 2018-12-27 | 2019-03-12 | 福建医科大学 | A kind of specimen preserving liquid and preparation method thereof |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
| CN111296408A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Specimen environment-friendly composite mildew-proof preservative of natural plant extract and application thereof |
| CN111226905A (en) * | 2020-03-25 | 2020-06-05 | 张家港市德仁科教仪器设备有限公司 | Novel environment-friendly specimen fixing composition, preparation method thereof and fixing liquid containing composition |
| CN114271261B (en) * | 2021-12-31 | 2022-12-30 | 广州市殡葬服务中心 | Efficient composite remains preservative and preparation method and preservation method thereof |
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| US3912809A (en) * | 1974-06-03 | 1975-10-14 | Champion Co | Disinfecting embalming composition |
| CN1751566A (en) * | 2004-09-23 | 2006-03-29 | 李慧 | Long-term antiseptic agent composition for remains, and prepn. method and application thereof |
| CN101036455A (en) * | 2007-01-10 | 2007-09-19 | 姜吉良 | Preservative and its preparation method |
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2008
- 2008-12-17 CN CN2008101439805A patent/CN101438698B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3912809A (en) * | 1974-06-03 | 1975-10-14 | Champion Co | Disinfecting embalming composition |
| CN1751566A (en) * | 2004-09-23 | 2006-03-29 | 李慧 | Long-term antiseptic agent composition for remains, and prepn. method and application thereof |
| CN101036455A (en) * | 2007-01-10 | 2007-09-19 | 姜吉良 | Preservative and its preparation method |
Non-Patent Citations (2)
| Title |
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| 姜吉良等.改良尸体防腐固定液的研究.《南方医科大学学报》.2008,第28卷(第9期),第1557-1558页. * |
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