CN102524240B - Efficient and environment-friendly remains preservative and application thereof - Google Patents
Efficient and environment-friendly remains preservative and application thereof Download PDFInfo
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- CN102524240B CN102524240B CN201010581997.6A CN201010581997A CN102524240B CN 102524240 B CN102524240 B CN 102524240B CN 201010581997 A CN201010581997 A CN 201010581997A CN 102524240 B CN102524240 B CN 102524240B
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Abstract
The invention relates to an efficient and environment-friendly remains preservative free of formaldehyde and application thereof. The remains preservative mainly comprises, by volume, 0.5-10% of phenoxyethanol, 0.2-1.5% of isothiazolinone, 3-15% of organic acid, 5-20% of propylene glycol, 5-30% of glycerol, 20-75% of ethanol and 5-35% of distilled water, and, by weight, 1-10% of hexamethylenetetramine and 0-2% of ethylenediamine tetraacetic acid disodium salt. The preservative can be poured through an aorta and an aortic arch and injected through the abdominal cavity, the thoracic cavity, the pharyngeal cavity and the cranial cavity so as to carry out antiseptic preservation, disinfection and smell removal to remains. Quantification sterilization experiments, animal experiments and funeral home field experiments prove that the remains preservative is efficient in antibacterial property, quick in action speed, low in toxicity and suitable for short-term or long-term remains antisepsis or specimen preservation of funeral homes and medical colleges at home and abroad.
Description
Technical field
The present invention relates to the Gentle Repose Techniques of preserving in field of funeral and interment and human dissection teaching and scientific research field, be specifically related to Gentle Repose agent and the application thereof of a kind of low toxicity, efficient, environmental protection.
Background technology
Gentle Repose is the researching human body death subject that remains are preserved afterwards, specifically refer to and within a period of time, adopt the method such as physics, chemistry to prevent or slow down further developing of the corrupt process of rotting of remains, make remains be kept at relatively good state and can prevent contingent disease propagation, to carry out funeral activities or anatomic teaching scientific research.At present, generally adopt the main component of 4% formaldehyde (i.e. 10% formalin) solution as remains (corpse) preservative both at home and abroad.Formalin is strong to the fixing induration of tissue, has the advantages such as permeability is strong, price is low, sterilizing power is strong, favorable anti-corrosion effect; But its obvious shortcoming is, organize hard and crisp, elasticity is little, anti-mold effect is poor, and formaldehyde has strong impulse smell, human body is had to serious harm (the bibliography 1:Naya M such as spread effect, sensitization, mutagenesis, et al.Regul.Toxicol.Pharm., 2005,43:232-248).
For reducing formaldehyde to environment and healthy harm, dissect educational circles and proposed a lot of improvement methods, comprise: the main component that changes impregnating fluid, the concentration (bibliography 2:ColemanR, et al.JAnat., 1998 that use formaldehyde substitute or reduce formaldehyde, 192:443-446. bibliography 3: Liu Yangbin, Deng. Chinese Tissue Engineering Study and clinical rehabilitation, 2007,11:4625-4626); The volatilization of inhibition formaldehyde (bibliography 4: Ding Guofang, etc. environment and occupational medicine, 2003,20:451-453), reduction or elimination formaldehyde odor (bibliography 5:HiipakkaD W, et al.AIHAJ, 2001,62:689-696) etc.
Application number is that 200810125376.X, 200510119111.5 Chinese invention patent disclose respectively taking formaldehyde and glutaraldehyde as main embalming and preserve liquid.Jiang Jiliang (200710034262.X) discloses a kind of preservative of the stronger anti-mildew effect being made up of main slaine, organic acid and crosslinking agent; Cao Jianming (200710005074.4) discloses a kind of composition for preserving corpse samples, formed by anticorrosive mildewproof active component (heterocyclic compound, quaternary ammonium salt), Osmolyte regulator and humectant, only for the fixing preserving corpse samples after treatment of warp.
In field of funeral and interment, Zhu Jinlong, the Ren Zuhan etc. of Shanghai Inst. of Embalming (Fangfu Science and Technology Co Ltd, Shanhai) preserves aspect anticorrosion and done many good tries at remains.It is the impregnating fluid that adopts the material formulations such as inorganic peroxy thing, glycerine, alcohol, plant (Chinese herbal medicine) extract that its invention (01132050.8) " Shen dragon board " remains are preserved impregnating fluid.The composition that Chinese patent application 200410066612.7 and 200410053606.8 discloses for long and short phase Gentle Repose.Foregoing preservatives has been used peroxide, easily produces gas, causes remains dilatancy, and its antiseptic effect is abundant not in high temperature and humidity environment.
In the world, Gentle Repose agent mainly contains: the serial preservatives such as U.S. Dodge, Spain Complucad, Italian FineFIX.Dodge aseptic applications is the widest, but generally contains formaldehyde.Complucad is containing formaldehyde, phenol, taking organic peroxide and ethanol as main component; FineFIX be Milestone patent carry out the novel without formalin fixative of morphology research, but these two kinds of preservatives prove that through field experiment antiseptic effect is general, are not suitable for China's actual conditions.
For overcoming the shortcoming taking formaldehyde as main remains (corpse) preservative, further improve the performance of existing preservative, to adapt to the anticorrosion preservation requirement of various environment and region, in the urgent need to developing the Gentle Repose agent of a kind of efficient, low toxicity, environmental protection, by methods such as remains arterial perfusion and body cavity suction injections, effectively prevent the propagation of growing of the interior pathogenic microorganism of remains, control the development of remains self-dissolving, corruption, make remains processing reach innoxious.
Summary of the invention
The object of the invention is to overcome the deficiency of existing preservative, provide one not containing formaldehyde, inorganic oxide and organic oxygen compound, and novel Gentle Repose agent and the application method thereof of efficient, low toxicity, environmental protection, preserve for funeral service and corpse medically (or tissue).
The object of the present invention is achieved like this:
Gentle Repose agent and the application thereof of a kind of low toxic and environment-friendly provided by the invention, is characterized in that:
Gentle Repose agent of the present invention, the hexa that the Phenoxyethanol that is mainly 0.5～10% by volume ratio, 0.2～1.5% isothiazolinone, 3～15% organic acid, 5～20% propane diols, 5～30% glycerine, 20～75% ethanol, 5～35% distilled water and weight ratio are 1～10%, 0～2% sodium ethylene diamine tetracetate form.
Isothiazolinone in preservative of the present invention is selected from CMIT and MIT, and organic acid is selected from acetic acid and propionic acid.
The preparation method of Gentle Repose agent of the present invention comprises the steps: by recipe ratio, and hexa is soluble in water, adds sodium ethylene diamine tetracetate, stirs, and fully dissolves; Then add while stirring ethanol, propane diols, glycerine, isothiazolinone, Phenoxyethanol and organic acid, mix and get final product.
For evaluating the killing effect of preservative of the present invention to microorganism, according to disinfection technology standard (version in 2002), carry out respectively Guantitative bactericidal test and fungi and killed test.Bacterial classification is selected: staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Candida albicans ATCC 10231.Are set as respectively working concentration and action time diluting 40 times and 6h.By working concentration and the action time of specifying, repeated test 3 times, suspension is quantitatively killed test (referring to table 1), to each time of staphylococcus aureus and Escherichia coli kill logarithm value equal >=5.00; What Candida albicans was tested for each time kills logarithm value all >=4.00, illustrates that preservative germicidal efficiency of the present invention is high, sterilizes qualified.
Table 1 is inoculated the each bacterium survival of different time sections concentration
Note: "/" represents not detect
Gentle Repose agent provided by the invention and application thereof, have following advantage:
Gentle Repose agent of the present invention does not contain formaldehyde, inorganic oxide and organic oxygen compound, and formaldehydeless penetrating odor is little to environment and human health risk, low toxicity, environmental protection.Its preparation method is easy, and raw material is easy to get, workable; Practical application is convenient, is beneficial to and promotes the use of.
In Gentle Repose agent component of the present invention, contain preservative, bactericide, anticoagulant, bleeding agent, humectant, solvent etc., rationally, ratio is appropriate for formula.Wherein, after isothiazolinone contacts with microorganism, work by the key that disconnects bacterium and algae protein matter the effect of killing livestock, promptly irreversibly suppress its growth, thereby cause the death of microbial cell, therefore common bacteria, fungi, algae etc. are had to very strong inhibition and killing action.Isothiazolinone germicidal efficiency is high, and degradability is good, has the feature such as do not produce residual, handling safety, compatibility is good, stability is strong, is the new type bactericide of generally acknowledge in the world efficient, low toxicity, broad spectrum activity.
Gentle Repose agent of the present invention has Gentle Repose, preservation, the smelly effect of sterilizing, dispel simultaneously, can pass through the perfusion of sustainer, the arch of aorta or abdomen/chest/pharynx/cranial cavity injection, to remains carry out disinfection, anticorrosion and preservation.In the time that corpse is processed, can not discharge remains inner blood, when operation, ooze out without corpse liquid, free from environmental pollution, remains after treatment only need be preserved under room temperature state, are the several years, do not need refrigerator cold-storage.
Gentle Repose agent of the present invention, in being applied to field of funeral and interment long (short) phase Gentle Repose, also can be applicable to that legal medical expert tracks down and arrest that appearance is restored, the zoological specimens preservation of medical colleges and schools etc., all has good antiseptic effect.
Further describe the present invention below in conjunction with drawings and Examples:
Brief description of the drawings
Accompanying drawing 1 is: the mouse tissue form pathological change microscope figure of injection preservative of the present invention.
(A) brain, (B) intestines, (C) kidney, (D) skeletal muscle.All amplify 400 times.
Embodiment
Embodiment 1: mouse is soaked anticorrosion preservation experiment.
In specimen bottle, each mouse peritoneal is injected after 10mL preservative of the present invention, and be soaked in this preservative, observe mouse antiseptic effect.With business Complucad and FineFIX preservative in contrast.Laboratory mice is purchased from Military Medical Science Institute's Experimental Animal Center, male and female half and half, body weight 30～35g.
Mouse is soaked anticorrosion preservation 7 months (on December 6,6 days～2010 May in 2010), uses the mouse color of preservative of the present invention normal, and good springiness is apparent without corruption, without fungus growth, preserves liquid clear; The mouse that uses FineFIX preservative, preserves liquid and occurs cotton-shaped limbs after 3 months, be unsuitable for long-term preservation; Use the mouse color jaundice of Complucad preservative, health is transparent, has serious atrophy phenomenon.Experimental result shows: preservative of the present invention is significantly better than Complucad and FineFIX preservative.
Embodiment 2: the anticorrosion experiment of laboratory injected in mice.
Use the speed of 0.1mL/1Kg to sleep after new II (for animals) execution one group of experiment mice, press the dosage injection of heart preservative of the present invention of 25mL/100g, be placed in the dry place of shady and cool ventilation, under room temperature state, deposit 30 days (on June 4,5 days～2010 May in 2010), observe antiseptic effect.In experiment the 3rd day, 7 days, 14 days, 30 days time, respectively get a mouse and dissects and draw materials, cut respectively the tissues such as skeletal muscle, intestines, the heart, lung, liver, kidney, brain, fixing, paraffin section, hematoxylin eosin staining, microscopic examination photograph.This experiment taking FineFIX preservative as contrast, using sterile saline as blank.Experiment mice is purchased from Military Medical Science Institute's Experimental Animal Center, male, body weight 150～200g.
Experimental result is as follows:
Overall appearance aspect, the 3rd day when dissected of physiological saline control group has been shown in stink, to the 7th day skin blackout, suppurates easily broken, rots serious, has foul odour; And stink does not all appear in the mouse of use preservative of the present invention and FineFIX preservative during to 30 days, there is the phenomenon of leakage in FineFIX mouse part, and by outward appearance and anatomic observations such as mouse hardness, colors, preservative of the present invention is significantly better than FineFIX preservative.
Mouse tissue form pathological section microscope result aspect, preservative of the present invention is also significantly better than FineFIX (referring to table 2).As seen from Table 2, use the mouse lung liver cell nuclear quantity of preservative of the present invention significantly more than FineFix group.From the mouse tissue form pathological change microscope figure (Fig. 1) of injection preservative of the present invention, mouse brain institutional framework is roughly normal, the clear or small part core disappearance of the core of nerve cell, and interstitial structure is clear; Each layer of great majority of intestinal tissue are normal, and cell nucleus is most of clear; Glomerulus inner cell core is clear, minority disappears, and renal cells core is clear, minority disappears, and interstitial structure is clear; Skeletal Muscle Cell core major part normally or all disappears, and myocyte's band is regional to disappear, and interstitial is most of normal.
Table 2 mouse lung liver cell nuclear quantity statistics result
| Group | Lung | Liver |
| Preservative of the present invention | 21.2±2.8(23.3-17.6) | 128.8±20.1(153.3-111.0) |
| FineFix | 6.0±5.1(11.3-0) | 65.3±66.5(160.6-0) |
| Blank | 2.1±3.1(7.0-0) | 0±0(0-0) |
Embodiment 3: funeral parlor Gentle Repose field experiment.
Utilize Gentle Repose agent of the present invention to apply 30 many cases (now lift three examples and list in table 3) in the funeral parlor, can pass through the perfusion of sustainer, the arch of aorta or abdomen/chest/injections such as pharynx/cranial cavity, to remains carry out disinfection, anticorrosion and preservation.General groundwater increment: 1500～3000mL, when injection: 5～30min, under the room temperature of the funeral parlor, deposit and efficiently in 5 days reach 100%, can make remains face keep sleep shape, without decortication, chest belly dyes and swelling phenomenon, odorless without green.
Table 3 preservative of the present invention funeral parlor field experiment result
Claims (3)
1. a Gentle Repose agent, is characterized in that the hexa that Phenoxyethanol that this preservative is mainly 0.5～10% by volume ratio, 0.2～1.5% isothiazolinone, 3～15% organic acid, 5～20% propane diols, 5～30% glycerine, 20～75% ethanol, 5～35% distilled water and weight ratio are 1～10%, 2% sodium ethylene diamine tetracetate form; Described preservative, is set as respectively working concentration and action time diluting 40 times and 6h;
Described isothiazolinone is selected from CMIT and MIT, and organic acid is selected from acetic acid and propionic acid.
2. the preparation method of preservative claimed in claim 1, is characterized in that the preparation method of this preservative comprises the steps:
By recipe ratio, hexa is soluble in water, add sodium ethylene diamine tetracetate, stir, fully dissolve; Then add while stirring ethanol, propane diols, glycerine, isothiazolinone, Phenoxyethanol and organic acid, mix and get final product.
3. preservative according to claim 1, is characterized in that by the perfusion of sustainer, the arch of aorta or abdomen/chest/pharynx/cranial cavity injection preservative, to remains carry out disinfection, anticorrosion and preservation.
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| CN104094923B (en) * | 2013-04-09 | 2016-06-15 | 民政部一零一研究所 | A kind of antiseptic preserving fluid for corpse, tissue and dissection and using method thereof |
| CN103988834A (en) * | 2014-05-18 | 2014-08-20 | 南阳医学高等专科学校 | Animal specimen preserving fluid |
| CN104365581B (en) * | 2014-11-05 | 2016-08-24 | 中山大学 | A kind of Novel specimen preserves liquid |
| CN104521942B (en) * | 2014-12-24 | 2016-04-27 | 博兴县殡仪馆 | A kind of Gentle Repose agent |
| CN104904706A (en) * | 2015-04-22 | 2015-09-16 | 青岛农业大学 | Formaldehyde-free animal specimen fixed liquid and preparation method thereof |
| CN106359367A (en) * | 2016-08-30 | 2017-02-01 | 河南新大阳生物技术有限公司 | Formaldehyde-free specimen tissue preserving fluid and preparation method thereof |
| CN109805001B (en) * | 2017-11-22 | 2021-01-01 | 哈尔滨格林标本技术开发有限公司 | Formaldehyde-free tissue fixation preservative solution and preparation method thereof |
| ES2725301B2 (en) * | 2018-03-23 | 2020-05-27 | Rodino Ramon Angel Soto | EXITUS CONSERVATION COMPOSITION, ITS MANUFACTURE AND ITS USE |
| CN108760443B (en) * | 2018-05-18 | 2021-03-30 | 漯河医学高等专科学校 | Fixing method of pathological specimen |
| CN109042621A (en) * | 2018-08-01 | 2018-12-21 | 张炳强 | A kind of dedicated short-term corrosion prevention liquid of gross specimen and preparation method thereof |
| CN111903660A (en) * | 2020-08-19 | 2020-11-10 | 中国水产科学研究院黄海水产研究所 | Method for preparing fish specimen |
| CN114503977B (en) * | 2022-01-26 | 2023-03-03 | 民政部一零一研究所 | Remains preservative with detumescence, fading and odor removing functions and preparation method thereof |
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