CN104904706A - Formaldehyde-free animal specimen fixed liquid and preparation method thereof - Google Patents
Formaldehyde-free animal specimen fixed liquid and preparation method thereof Download PDFInfo
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- CN104904706A CN104904706A CN201510200832.2A CN201510200832A CN104904706A CN 104904706 A CN104904706 A CN 104904706A CN 201510200832 A CN201510200832 A CN 201510200832A CN 104904706 A CN104904706 A CN 104904706A
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Abstract
The invention discloses a formaldehyde-free animal specimen fixed liquid and a preparation method thereof, the formaldehyde-free animal specimen fixed liquid includes the following components: a biocide mildewcide, a protein denaturant, a polyol moisturizing agent and balance of water, and if the biocide mildewcide is in solid state, the mass concentration of the biocide mildewcide is 5-20 g/L, if the biocide mildewcide is liquid, the volume concentration of the biocide mildewcide is 1-2%; the volume concentration of the protein denaturant is 5%-40%; and the volume concentration of the polyol moisturizing agent is 2% to 10%. The formaldehyde-free animal specimen fixed liquid does not contain formaldehyde, and is non-volatile, and harmless to the human body; a fresh animal organ is washed with normal saline, and stored in the fixed liquid, a year later, the organ is not corrupted, free of mildew spot, and free of obvious color and shape change, the fixed liquid color has no obvious change, and the storage effect is good.
Description
Technical field
The present invention relates to zoological specimens fixer, relate to a kind of formaldehydeless zoological specimens fixer and preparation method thereof specifically.
Background technology
Animal tissue is subject to the effect of desmoenzyme (lysosomal enzyme) and causes each histoorgan deliquescing or liquefaction after animal dead, and this phenomenon is self-dissolving.The self-dissolvings such as each organ midbrain, spinal cord, pancreas and suprarenal gland occur very fast, and even cell structure disappears completely; The self-dissolving such as muscle, liver,spleen,kidney shows as quality deliquescing, structural fuzzy, and last cytolysis disappears; The organ such as stomach, intestines also can dissolve because of the effect of digestive juice.Bacterium in animal body and the bacterium in environment are understood amount reproduction and cause the corruption of protein in tissue to decompose after animal dead in addition.Therefore the conserving liquid of sample will have the effect of sterilization, mildew-resistant and protein denaturation.
At present, the preservation of zoological specimens generally uses formalin (formalin).The infiltration capacity of formalin is strong, can combine, make protein coagulating, favorable anti-corrosion effect with amino in protein.But the volatility of formaldehyde is extremely strong, there is strong impulse smell, can cause damage to the multiple organ of people.In June, 2004, formaldehyde is risen to 1 class carcinogen by 2 class carcinogens by No. 153 bulletins of international cancer research institution of the World Health Organization (IARC).Due to the strong volatility of formaldehyde, eye conjunctiva and the respiratory mucosa of contact personnel can be stimulated, cause a shouting pain, drop tears, dizzy, skin contact can cause allergy, dermatitis and eczema, Long Term Contact also may cause cancer, and serious harm contact personnel's is healthy; In formalin, the long-term sample preserved also can be hardening, fades.In addition due to the volatility of formaldehyde, in the process of Saving specimen, need regular replenishment or replacing.
Ethanol (alcohol) is the disinfectant medically commonly used.For concentration time fixing with 80-95% as well, there is the effects such as protein coagulation, sclerosis, dehydration.Organ that the self-dissolving of histoorgan and microorganism can be avoided to cause is corrupt, but its penetration is more weak, when fixing with alcohol in high concentration, can make to organize hardening, shrink larger.
Patent CN 200910003987.1 discloses a kind of cadaver-preservative fluid containing diacetate, nitrate, polyalcohol.Patent CN 1233391 A discloses a kind of preservation liquid for animal body specimen formed containing sodium salt or sylvite preservative, polyhydroxy class color preserving agent and water, respectively using diacetate and sodium chloride, Sodium Benzoate, potassium chloride, potassium nitrite etc. as preservative, using the polyalcohol such as ethylene glycol, glycerine as color preserving agent.These two kinds of conserving liquid all lack protein denaturant, well can not preserve gross specimen and internal organ sample.Patent CN 102175496 B discloses formaldehyde-free fixative for tissue sample, mainly comprises the glacial acetic acid of ethanol 71%-79%, 2-4%, the polyalcohol of 4-6%, the surfactant of 4-6%.The main component of this fixer is ethanol, and its penetration is poor, poor to large organ fixed effect.
Summary of the invention
Technical problem to be solved by this invention, is just to provide a kind of not containing zoological specimens fixer and preparation method thereof of formaldehyde and volatile reagent.
In order to solve the problems of the technologies described above, the present invention adopts following technical scheme:
A kind of formaldehydeless zoological specimens fixer, its improvements are, comprise following composition: biocide mildewcide, protein denaturant, polyalcohol humectant and water, if wherein biocide mildewcide is solid-state, then its mass concentration is 5-20g/L, if biocide mildewcide is liquid, then its volumetric concentration is 1-2%; The volumetric concentration of protein denaturant is 5%-40%; The volumetric concentration of polyalcohol humectant is 2%-10%; Surplus is water.
Further, described biocide mildewcide is shitosan, quaternary ammonium salt surface active agent, potassium sorbate, Na
2one or more in EDTA.
Further, described shitosan is chitosan lactate, and described quaternary ammonium salt surface active agent is one or more in dodecyl dimethyl benzyl ammonium bromide, Dodecyl trimethyl ammonium chloride, didecyldimethylammonium bromide.
Further, described protein denaturant is one or more in ethanol, acetic acid, acetone, and wherein the volumetric concentration of ethanol is 5%-40%, the volumetric concentration of acetic acid is 0-2%, the volumetric concentration of acetone is 0-2%.
Further, described polyalcohol humectant is one or more in ethylene glycol, diethylene glycol, propane diols, glycerine.
A preparation method for formaldehydeless zoological specimens fixer, its improvements are, comprise the steps:
(1) take chitosan lactate 5-20g or potassium sorbate 5-20g, be dissolved in the water, add the Na of 1-3g
2eDTA, stirs;
(2) add the quaternary surfactant of 1-2% volumetric concentration, 95% ethanol of 5%-40% volumetric concentration, the reagent such as acetic acid, the acetone of 0-2% volumetric concentration, the diethylene glycol of 2%-10% volumetric concentration of 0-2% volumetric concentration respectively, stir;
(3) add water and be settled to 1000ml.
The invention has the beneficial effects as follows:
(1) zoological specimens fixer formula disclosed in this invention is not containing formaldehyde, the reagent of non-volatility.After fresh animal organ normal saline flushing, be kept in this fixer, after 1 year, organ is without corruption, and without mildew, color and luster, shape are without significant change, and fixer color is without significant change, and preservation effect is good.
(2) what zoological specimens fixer disclosed in this invention may be used in the preservation of Zootomy organ and animal histology's histologic section manufacturing process is fixing, it is not containing formaldehyde, so non-volatility smell, harmless, student also can not be unwilling forward because of excitant, teaching efficiency is good.
Embodiment
Embodiment 1:
Shitosan 5g, Dodecyl trimethyl ammonium chloride 10ml, 95% ethanol 100ml, acetic acid 20ml, diethylene glycol 10ml are added water to 1000ml, namely obtains formaldehydeless sample fixer.
Embodiment 2:
Shitosan 5g, Dodecyl trimethyl ammonium chloride 50ml, 95% ethanol 100ml, acetic acid 20ml, diethylene glycol 10ml, glycerine 10ml are added water to 1000ml, namely obtains formaldehydeless sample fixer.
Embodiment 3:
By shitosan 5g, didecyldimethylammonium bromide 25ml, 95% ethanol 100ml, trichloroacetic acid (5%) 20ml, glycerine 10ml add water to 1000ml, namely obtain formaldehydeless sample fixer.
Embodiment 4:
By shitosan 5g, didecyldimethylammonium bromide 25ml, 95% ethanol 100ml, acetic acid 20ml, glycerine 10ml add water to 1000ml, namely obtain formaldehydeless sample fixer.
Embodiment 5:
By shitosan 5g, didecyldimethylammonium bromide 25ml, 95% ethanol 200ml, acetic acid 20ml, glycerine 10ml add water to 1000ml, namely obtain formaldehydeless sample fixer.
Embodiment 6:
By potassium sorbate 10g, Dodecyl trimethyl ammonium chloride 50ml, Na
2eDTA 10g, 95% ethanol 100ml, glycerine 10ml add water to 1000ml, namely obtain formaldehydeless sample fixer.
Embodiment 7:
By potassium sorbate 10g, dodecyl dimethyl benzyl ammonium bromide 50ml, Na
2eDTA 10g, 95% ethanol 100ml, glycerine 10ml add water to 1000ml, namely obtain formaldehydeless sample fixer.
Preservation effect:
The formaldehydeless sample fixer of embodiment 1 is adopted to preserve the liver organization of rabbit.On June 16th, 2013 preserves, and observes preservation effect after 1 year.After rabbit fresh liver tissue normal saline flushing, be kept in this fixer, after 1 year, liver is without corruption, and without mildew, color and luster, shape are without significant change, and fixer color is without significant change, and preservation effect is good.And solution colour turns yellow in the wide-mouth bottle that 70% alcohol is preserved.
Claims (6)
1. a formaldehydeless zoological specimens fixer, is characterized in that, comprises following composition: biocide mildewcide, protein denaturant, polyalcohol humectant and water, if wherein biocide mildewcide is solid-state, then its mass concentration is 5-20g/L, if biocide mildewcide is liquid, then its volumetric concentration is 1-2%; The volumetric concentration of protein denaturant is 5%-40%; The volumetric concentration of polyalcohol humectant is 2%-10%; Surplus is water.
2. formaldehydeless zoological specimens fixer according to claim 1, is characterized in that: described biocide mildewcide is shitosan, quaternary ammonium salt surface active agent, potassium sorbate, Na
2one or more in EDTA.
3. formaldehydeless zoological specimens fixer according to claim 2, it is characterized in that: described shitosan is chitosan lactate, described quaternary ammonium salt surface active agent is one or more in dodecyl dimethyl benzyl ammonium bromide, Dodecyl trimethyl ammonium chloride, didecyldimethylammonium bromide.
4. formaldehydeless zoological specimens fixer according to claim 1, it is characterized in that: described protein denaturant is one or more in ethanol, acetic acid, acetone, wherein the volumetric concentration of ethanol is 5%-40%, the volumetric concentration of acetic acid is 0-2%, the volumetric concentration of acetone is 0-2%.
5. formaldehydeless zoological specimens fixer according to claim 1, is characterized in that: described polyalcohol humectant is one or more in ethylene glycol, diethylene glycol, propane diols, glycerine.
6. a preparation method for formaldehydeless zoological specimens fixer, is characterized in that, comprise the steps:
(1) take chitosan lactate 5-20g or potassium sorbate 5-20g, be dissolved in the water, add the Na of 1-3g
2eDTA, stirs;
(2) add the quaternary surfactant of 1-2% volumetric concentration, 95% ethanol of 5%-40% volumetric concentration, the reagent such as acetic acid, the acetone of 0-2% volumetric concentration, the diethylene glycol of 2%-10% volumetric concentration of 0-2% volumetric concentration respectively, stir;
(3) add water and be settled to 1000ml.
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| CN201510200832.2A CN104904706A (en) | 2015-04-22 | 2015-04-22 | Formaldehyde-free animal specimen fixed liquid and preparation method thereof |
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| CN201510200832.2A CN104904706A (en) | 2015-04-22 | 2015-04-22 | Formaldehyde-free animal specimen fixed liquid and preparation method thereof |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340875A (en) * | 2015-12-16 | 2016-02-24 | 贵阳中医学院 | Specimen preserving fluid and preparation method thereof |
| CN105360108A (en) * | 2015-12-16 | 2016-03-02 | 贵阳中医学院 | Specimen preservation fluid good in preservation effect and preparation method of specimen preservation fluid |
| CN105823665A (en) * | 2016-05-13 | 2016-08-03 | 北京九州柏林生物科技有限公司 | Biological tissue sample preparation sleeve solution |
| CN105842033A (en) * | 2016-05-13 | 2016-08-10 | 北京九州柏林生物科技有限公司 | Formaldehyde-free tissue specimen stationary liquid |
| CN106257567A (en) * | 2016-03-11 | 2016-12-28 | 宁夏大学 | Lumbricus section and manufacture method and application thereof for microsurgery training |
| CN106706395A (en) * | 2016-12-08 | 2017-05-24 | 武汉宏兹生物技术有限公司 | Novel environment-friendly stationary liquid |
| CN107593684A (en) * | 2017-09-29 | 2018-01-19 | 肇庆医学高等专科学校 | A kind of specimen preserving liquid and preparation method thereof |
| CN108353889A (en) * | 2018-03-29 | 2018-08-03 | 蚌埠医学院 | A kind of Traditional-Chinese-medicine-type nano-titanium dioxide specimen preserving liquid |
| CN108760443A (en) * | 2018-05-18 | 2018-11-06 | 漯河医学高等专科学校 | A kind of fixing means of Pathologic specimen |
| CN108967414A (en) * | 2017-05-31 | 2018-12-11 | 王虎 | USBU is without formalin fixer and its preparation method and application |
| CN109042622A (en) * | 2018-09-28 | 2018-12-21 | 河南科技大学 | A kind of guarantor's color fixing agent and preparation method thereof and the application in prepared by sample |
| CN109166439A (en) * | 2018-10-30 | 2019-01-08 | 河南科技大学 | A kind of production method of the stomach section silica gel model of sheep |
| CN109169634A (en) * | 2018-10-11 | 2019-01-11 | 内蒙古科技大学包头医学院 | The preservation liquid of human anatomy internal organ sample |
| CN109377846A (en) * | 2018-10-11 | 2019-02-22 | 内蒙古科技大学包头医学院 | The production method of human anatomy internal organ sample |
| CN109805001A (en) * | 2017-11-22 | 2019-05-28 | 哈尔滨格林标本技术开发有限公司 | Formaldehydeless tissue Fixed and Antiseptic liquid of one kind and preparation method thereof |
| CN111296407A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Environment-friendly composite mildew-proof preservative and application thereof |
| CN111387199A (en) * | 2020-02-17 | 2020-07-10 | 广东海洋大学深圳研究院 | Efficient composite mildew-proof preservative and application thereof |
| CN113519505A (en) * | 2021-09-01 | 2021-10-22 | 宁波西敦医药包衣科技有限公司 | Environment-friendly water-based cross-linking agent biological tissue preservation material and preparation method thereof |
| CN113916626A (en) * | 2021-09-29 | 2022-01-11 | 中南大学湘雅医院 | Pathological specimen fixing vehicle |
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| CN102524240A (en) * | 2010-12-10 | 2012-07-04 | 民政部一零一研究所 | Efficient and environment-friendly remains preservative and application thereof |
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Cited By (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340875A (en) * | 2015-12-16 | 2016-02-24 | 贵阳中医学院 | Specimen preserving fluid and preparation method thereof |
| CN105360108A (en) * | 2015-12-16 | 2016-03-02 | 贵阳中医学院 | Specimen preservation fluid good in preservation effect and preparation method of specimen preservation fluid |
| CN106257567A (en) * | 2016-03-11 | 2016-12-28 | 宁夏大学 | Lumbricus section and manufacture method and application thereof for microsurgery training |
| CN106257567B (en) * | 2016-03-11 | 2020-04-10 | 宁夏大学 | Earthworm section for microsurgery training, and manufacturing method and application thereof |
| CN105842033A (en) * | 2016-05-13 | 2016-08-10 | 北京九州柏林生物科技有限公司 | Formaldehyde-free tissue specimen stationary liquid |
| CN105823665A (en) * | 2016-05-13 | 2016-08-03 | 北京九州柏林生物科技有限公司 | Biological tissue sample preparation sleeve solution |
| CN106706395A (en) * | 2016-12-08 | 2017-05-24 | 武汉宏兹生物技术有限公司 | Novel environment-friendly stationary liquid |
| CN106706395B (en) * | 2016-12-08 | 2021-03-02 | 横琴宏恩医疗科技有限公司 | Environment-friendly fixing liquid |
| CN108967414A (en) * | 2017-05-31 | 2018-12-11 | 王虎 | USBU is without formalin fixer and its preparation method and application |
| CN107593684A (en) * | 2017-09-29 | 2018-01-19 | 肇庆医学高等专科学校 | A kind of specimen preserving liquid and preparation method thereof |
| CN109805001A (en) * | 2017-11-22 | 2019-05-28 | 哈尔滨格林标本技术开发有限公司 | Formaldehydeless tissue Fixed and Antiseptic liquid of one kind and preparation method thereof |
| CN109805001B (en) * | 2017-11-22 | 2021-01-01 | 哈尔滨格林标本技术开发有限公司 | Formaldehyde-free tissue fixation preservative solution and preparation method thereof |
| CN108353889B (en) * | 2018-03-29 | 2021-05-25 | 蚌埠医学院 | Traditional Chinese medicine type nano titanium dioxide specimen preserving fluid |
| CN108353889A (en) * | 2018-03-29 | 2018-08-03 | 蚌埠医学院 | A kind of Traditional-Chinese-medicine-type nano-titanium dioxide specimen preserving liquid |
| CN108760443A (en) * | 2018-05-18 | 2018-11-06 | 漯河医学高等专科学校 | A kind of fixing means of Pathologic specimen |
| CN109042622A (en) * | 2018-09-28 | 2018-12-21 | 河南科技大学 | A kind of guarantor's color fixing agent and preparation method thereof and the application in prepared by sample |
| CN109169634A (en) * | 2018-10-11 | 2019-01-11 | 内蒙古科技大学包头医学院 | The preservation liquid of human anatomy internal organ sample |
| CN109377846A (en) * | 2018-10-11 | 2019-02-22 | 内蒙古科技大学包头医学院 | The production method of human anatomy internal organ sample |
| CN109166439B (en) * | 2018-10-30 | 2020-11-13 | 河南科技大学 | Method for manufacturing sheep gastric section silica gel model |
| CN109166439A (en) * | 2018-10-30 | 2019-01-08 | 河南科技大学 | A kind of production method of the stomach section silica gel model of sheep |
| CN111387199A (en) * | 2020-02-17 | 2020-07-10 | 广东海洋大学深圳研究院 | Efficient composite mildew-proof preservative and application thereof |
| CN111296407A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Environment-friendly composite mildew-proof preservative and application thereof |
| CN113519505A (en) * | 2021-09-01 | 2021-10-22 | 宁波西敦医药包衣科技有限公司 | Environment-friendly water-based cross-linking agent biological tissue preservation material and preparation method thereof |
| CN113916626A (en) * | 2021-09-29 | 2022-01-11 | 中南大学湘雅医院 | Pathological specimen fixing vehicle |
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