CN104094923B - A kind of antiseptic preserving fluid for corpse, tissue and dissection and using method thereof - Google Patents
A kind of antiseptic preserving fluid for corpse, tissue and dissection and using method thereof Download PDFInfo
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Abstract
The present invention provides a kind of antiseptic preserving fluid for corpse, tissue and dissection and using method thereof. The antiseptic preserving fluid of the present invention mainly by the glutaraldehyde that mass percent is 0.5～2%, 0.3～3% thymol, 0.5～3% sodium benzoate, 0.1～1% sodium citrate or citric acid, 0.5～3% mannitol or D-glucitol and ethanol that volume ratio is 40～65%, 5～15% glycerol, 1～5% organic acid, the essence of 0～1%, the water of 14～54% constitutes. The present invention can adopt infusion method or perfusion that corpse, tissue and dissection are carried out anticorrosion and fix, and plays anticorrosion, preservation, sterilization, deodorant effect. Antiseptic preserving fluid of the present invention, has passed through laboratory animal experiments and funeral parlor field experimentation test, has had Efficient antibacterial, safety and low toxicity, it is adaptable to the anticorrosion of medical teaching scientific research and the corpse in funeral services, tissue and dissection preserves.
Description
Technical field
The present invention relates to the embalming Techniques of preserving in human dissection teaching and scientific research field and field of funeral and interment, be specifically related to a kind of antiseptic preserving fluid for corpse, tissue and dissection and using method thereof.
Background technology
Recognize organization of human body, seek Know Your Body and the demand of modern funeral service, be unable to do without obduction and histiocytic research, it is desirable to have the anticorrosion for corpse, tissue and organ and the Techniques of preserving of a set of science. After death, if corpse processes without anticorrosion is fixing, tissue automatic soup-dissolving will soon be there is, rot in human or animal. Civil administration funeral service unit, when carrying out the international fortune business such as corpse, remains preservation, also often uses Gentle Repose technology.
Conventional embalming store method has: medicine anticorrosion, vacuum or noble gas anticorrosion, dry anticorrosion, Excised Embryos etc. For fully keeping the shape characteristic of corpse and tissue, no matter at medical domain or in funeral and interment department, commonly use medicine (being generally formaldehyde) anti-corrosion method both at home and abroad. But it is hard and crisp, elastic little that formaldehyde and antiseptic preservation method has tissue, and anti-mold effect is poor, and has strong impulse abnormal smells from the patient, and human body has the serious harm such as sensitization, mutagenic action.
For reducing formaldehyde to environment and healthy harm, research worker proposes a lot of modification method, as reduced the concentration of formaldehyde, suppressing (list of references: the Yang Dehui such as formaldehyde volatilization, elimination formaldehyde odor, use formaldehyde succedaneum, Deng. a kind of isothiazolone compound recipe preservative Cavia porcellus preservative challenge test effect observation, Chinese Clinical Chinese Journal of anatomy, 2011,29 (4): 103-106).
Compared to formaldehyde, it is relatively big that glutaraldehyde (Glutaraldehyde) has molecule, and not volatile, penetrating odor is little, and it is strong like that glutaraldehyde is also not so good as formaldehyde with the reaction of protein.Glutaraldehyde belongs to high effect disinfectants, has the feature such as wide spectrum, efficient, low toxicity, metal protection is little, little by organic substance influence, good stability. Glutaraldehyde and albumino reaction have a wider pH value range, and this is a critically important advantage for anticorrosion. Because of people after death, from death to anticorrosion during this period of time in, the pH value of the different parts of body is different. It was verified that glutaraldehyde is better than formaldehyde as preservative, but its price is more expensive than formaldehyde. Answer rational allocation antiseptic preserving fluid component, reduce formulation cost. Zhao Duo etc. disclose cadaver-preservative fluid and preparation method thereof (number of patent application 200510119111.5), are characterized in being made up of 50% glutaraldehyde, the rare fatty alcohol ether of polyoxy second, ethanol, benzalkonium bromide, strong disinfecting preservative, BAIMEISHA fungusproof anti-corrosion agent, glycerol, water.
A kind of desirably for the antiseptic preserving fluid of corpse, tissue and dissection, should have low toxicity, zest is little, it is simple, cheap to use, anticorrosion internal validity strong, the specimen shrinkage factor that preserves is little, color and luster is close to features such as condition of living organism. For overcoming the existing shortcoming based on formaldehyde, remains (corpse) preservative of peroxide, improve the performance of existing antiseptic preserving fluid further, the anticorrosion adapting to various environment and region preserves requirement, in the urgent need to develop a kind of efficiently, low toxicity, environmental protection the antiseptic preserving fluid for corpse, tissue and dissection, by infusion method or the method such as remains arterial perfusion, body cavity aspiration injection, what effectively prevent pathogenic microorganism in remains grows propagation, control remains self-dissolving, corrupt development, be beneficial to medical anatomy scientific research or funeral parlor Gentle Repose preserves.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that the antiseptic preserving fluid of a kind of efficient, environmental protection for corpse, tissue and dissection and using method thereof, the anticorrosion to be applied to funeral service and corpse medically, tissue and dissection preserves.
The object of the present invention is achieved like this:
A kind of antiseptic preserving fluid for corpse, tissue and dissection provided by the invention and using method thereof, is characterized in that:
The antiseptic preserving fluid of the present invention mainly by the glutaraldehyde that mass percent is 0.5～2%, 0.3～3% thymol, 0.5～3% sodium benzoate, 0.1～1% sodium citrate (or citric acid), 0.5～3% mannitol (or D-glucitol) and ethanol that volume ratio is 40～65%, 5～15% glycerol, 1～5% organic acid, the essence of 0～1%, the water of 14～54% constitutes.
Antiseptic preserving fluid of the present invention, without formaldehyde, inorganic oxide and organic oxygen compound; The preferred acetic acid of organic acid and propanoic acid, the edible essence such as the preferred Fructus Ananadis comosi of essence, Fructus Citri tangerinae.
The preparation method of antiseptic preserving fluid of the present invention comprises the steps: by recipe ratio, and sodium benzoate is soluble in water, is sequentially added into sodium citrate (or citric acid), mannitol (or D-glucitol), stirring, fully dissolves; Then add ethanol, glutaraldehyde, thymol, glycerol, organic acid and essence while stirring, mix and get final product.
Antiseptic preserving fluid of the present invention can adopt infusion method or perfusion that corpse, tissue and dissection anticorrosion are fixed, by aorta, aortic arch perfusion or abdomen/breast/pharynx/cranial cavity injection preservative, play remains effective anticorrosion, preservation, sterilization, smelly effect rapidly.
Antiseptic preserving fluid provided by the invention and using method thereof, have an advantage that
This preservative is without formaldehyde, inorganic oxide and organic oxygen compound, and zest is little, and environment and human health risk is little.Its preparation method is easy, and raw material is easy to get, workable; Practical application is convenient, is beneficial to and promotes the use of.
The present invention is compound recipe antiseptic preserving fluid, and containing preservative, disinfectant, anticoagulant, penetrating agent, wetting agent etc. in its component, formula is reasonable, and ratio is suitable, and each component plays synergism.
Utilize antiseptic preserving fluid of the present invention can discharge blood in remains or not blood-letting when processing remains, should be decided according to the actual requirements. The anti-corrosion method of standard is otch, blood-letting in the world; If not discharging blood in remains, oozing out without corpse liquid during operation, free from environmental pollution, funeral genus is more easily accepted by.
Antiseptic preserving fluid of the present invention, has Efficient antibacterial, safety and low toxicity, it is adaptable to the anticorrosion of medical teaching scientific research and the corpse in funeral services, tissue and dissection preserves.
Accompanying drawing explanation
Fig. 1 is: each tissue slice microscope figure (200 ×) after medical college embalming
(A) cardiac muscle, (B) liver, (C) spleen, (D) lungs, (E) kidney, (F) coat of the stomach, (G) small intestinal, (H) large intestine, (I) skin.
Detailed description of the invention
Embodiment 1: soak anticorrosion and preserve experiment
After each mouse peritoneal is injected 10mL antiseptic preserving fluid of the present invention by Specimen bottle, it is soaked in this preservative, observes mice antiseptic effect. Using Spain's Complucad preservative as comparison, using sterile saline as blank. Laboratory mice is purchased from Military Medical Science Institute's Experimental Animal Center, male, strain: KM, body weight 29～34g.
Mice is soaked anticorrosion and preserves 12 months (2010～2011), and the mice color and luster using antiseptic preserving fluid of the present invention is normal, and good springiness is apparent without corruption, without fungus growth, preserves liquid and omits yellow, clear; The mouse body using Complucad preservative is transparent, has severe atrophy phenomenon; Mice blackening when saline control group the 7th day is observed, mouldy, there is strong odor, limbs crush gradually, then abandon in the 10th day. Test result indicate that: antiseptic preserving fluid of the present invention is significantly better than Complucad preservative.
Embodiment 2: laboratory rat injection preservative challenge test
By three groups of experimental rat places after death, inject preservative by the dose cardiac of 25mL/100g, be placed in shady and cool ventilation and dry place, deposit under room temperature state 30 days, observe antiseptic effect. When testing the 3rd day, 7 days, 14 days, 30 days, respectively taking a rat and carry out dissection and draw materials, cut the tissues such as skeletal muscle, intestinal, the heart, lung, liver, kidney, brain respectively, fixing, paraffin section, hematoxylin-eosin staining, microscope is observed and takes a picture. This experiment is with FineFIX preservative for comparison (FineFIX be Italy's Milestone patent carry out the novel without formalin fixative of Senile Mouse), using sterile saline as blank. Experimental rat is the Wistar rat of cleaning grade, purchased from Military Medical Science Institute's Experimental Animal Center, male, about body weight 150g.
Experimental result is as follows: overall appearance aspect, and the 3rd day when dissected of saline control group has been shown in stink, turns black to the 7th day skin, suppurates fragile, rots serious, has foul odour; And use preservative of the present invention and the mice of FineFIX preservative, during to 30 days, stink does not all occur, preservative mice color and luster of the present invention is closer to condition of living organism, there is the phenomenon of leakage in FineFIX mice part, by outward appearance and anatomic observations such as mice hardness, colors, preservative of the present invention is significantly better than FineFIX preservative.Mouse tissue form pathological section microscope is observed, and each organizational structure of mice is substantially normal, and nucleus is clear or small part core disappears, and interstitial structure is clear, illustrates that antiseptic preserving fluid of the present invention is also better than FineFIX. From table 1, the mouse lung liver cell nuclear quantity of antiseptic preserving fluid of the present invention is used to be significantly more than FineFIX group.
Table 1 mouse lung, liver cell nuclear quantity statistics result
| Group | Lung | Liver |
| Antiseptic preserving fluid of the present invention | 9.3±4.5(17.0-3.0) | 44.2±65.9(175.3-0) |
| FineFIX | 6.0±5.1(11.3-0) | 65.3±66.5(160.6-0) |
| Blank | 2.1±3.1(7.0-0) | 0±0(0-0) |
Embodiment 3: medical college Gentle Repose is tested
Choose tool adult male remains (body weight is about 60Kg), arterial perfusion and splanchnocoel injection antiseptic preserving fluid of the present invention in certain medical university and be about 25000mL, be positioned in the environment of 4 DEG C, close observation preservative challenge test result. After 50 days, from visual inspection remains without obvious corruption, odorless, core respectively, liver, spleen, lung, kidney, stomach, large intestine, small intestinal and skin, cut into slices with after parafilm wrap, HE dyes, and is observed by high power lens. As seen from the figure, antiseptic preserving fluid of the present invention is used substantially can to keep each cell of human body, the intrinsic form of tissue and structure.
Embodiment 4: funeral parlor Gentle Repose field experimentation
Corrosion protection preservative fluid for reliquiae of the present invention applies 30 many cases in the south China funeral parlor, is irrigated by femoral artery, brachial artery and is aided with abdomen/breast/pharyngeal cavity injection antiseptic preserving fluid and is about 2500mL, remains is carried out disinfection, anticorrosion and preservation. Deposit effective percentage in 5 days under room temperature in summer and reach more than 90%, it is possible to making remains face keep sleep shape, without decortication, thorax abdomen is without green dye and swelling, odorless.
Claims (3)
1. the antiseptic preserving fluid for corpse, tissue and dissection, it is characterised in that: antiseptic preserving fluid mainly by the glutaraldehyde that mass percent is 0.5～2%, 0.3～3% thymol, 0.5～3% sodium benzoate, 0.1～1% sodium citrate or citric acid, 0.5～3% mannitol or D-glucitol and ethanol that volume ratio is 40～65%, 5～15% glycerol, 1～5% organic acid, the essence of 0～1%, the water of 14～54% constitutes; Organic acid is acetic acid and propanoic acid, and essence is Fructus Ananadis comosi, Fructus Citri tangerinae edible essence.
2. antiseptic preserving fluid according to claim 1, it is characterised in that: the preparation method of this antiseptic preserving fluid comprises the steps:
By formula proportion, sodium benzoate is soluble in water, it is sequentially added into sodium citrate or citric acid, mannitol or D-glucitol, stirring, fully dissolve; Then add ethanol, glutaraldehyde, thymol, glycerol, organic acid and essence while stirring, mix and get final product.
3. the antiseptic preserving fluid according to claim 1 application in medical teaching scientific research and funeral services, it is characterized in that: adopt infusion method, arterial perfusion method or body cavity injection corpse, tissue and dissection to be carried out anticorrosion and fixes, play anticorrosion, preservation, sterilization, deodorant effect.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105532637A (en) * | 2016-01-07 | 2016-05-04 | 别永信 | Human body teaching specimen preserving fluid and preparing method thereof |
| CN105519520B (en) * | 2016-01-21 | 2018-01-09 | 中国人民解放军第四军医大学 | A kind of tissue specimen preserving fluid |
| CN106359367A (en) * | 2016-08-30 | 2017-02-01 | 河南新大阳生物技术有限公司 | Formaldehyde-free specimen tissue preserving fluid and preparation method thereof |
| CN107183004A (en) * | 2017-06-21 | 2017-09-22 | 上海于泽生物科技有限公司 | A kind of dissec antiseptic preserving fluid |
| CN107568200A (en) * | 2017-09-29 | 2018-01-12 | 肇庆医学高等专科学校 | A kind of store method of anatomy sample |
| CN107593684A (en) * | 2017-09-29 | 2018-01-19 | 肇庆医学高等专科学校 | A kind of specimen preserving liquid and preparation method thereof |
| CN108931408B (en) * | 2018-06-15 | 2021-08-27 | 山东聚众数字医学科技开发有限公司 | Color-preserving fixing liquid for dissecting teaching specimen and preparation method thereof |
| CN110169417A (en) * | 2019-04-30 | 2019-08-27 | 黄恩南 | Deoderizing Gentle Repose liquid |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
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