CN102461499A - Disinfector for remains and preparation method thereof - Google Patents
Disinfector for remains and preparation method thereof Download PDFInfo
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- CN102461499A CN102461499A CN2010105466620A CN201010546662A CN102461499A CN 102461499 A CN102461499 A CN 102461499A CN 2010105466620 A CN2010105466620 A CN 2010105466620A CN 201010546662 A CN201010546662 A CN 201010546662A CN 102461499 A CN102461499 A CN 102461499A
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Abstract
The invention relates to a high-efficient, low-toxicity and environmental-friendly disinfector for remains and a preparation method thereof. The disinfector comprises the following components in percentage by mass: 0.4-0.6 percent of o-phthaldialdehyde, 0.05-0.1 percent of benzalkonium bromide, 0.2-0.5 percent of polyethylene glycol monooleyl ether, 2-10 percent of ethanol, 1-3 percent of isopropanol, 0.25-1.5 percent of organic acid, 0-0.3 percent of essence and 84-96 percent of distilled water. As demonstrated by a stability test, a disinfection microorganism test (including a neutralizer appraisal test) and a funeral parlour field disinfection trial, the disinfector provided by the invention has the advantages of good stability, high sterilization efficiency, no stimulation or corrosivity, etc., and can be used for disinfecting the remains, relevant environments, air and general object surfaces.
Description
Technical field
The present invention relates to a kind of chemosterilant, be specifically related to remains disinfectant of a kind of high-efficiency low-toxicity that can be applicable to corpse and relevant environment thereof and preparation method thereof.
Background technology
The funeral and interment place is a special public place, and remains are major pollution sources.The various pathogenic microorganisms that remains carry like saprophytic bacteria and (condition) pathogenic bacteria etc., can be spread out in each process of funeral service, cause microbial contaminations such as air in the environment, body surface, remains surface, worker's watch face.Remains meet fortune worker, anticorrosion lift face worker, explosive industry all directly contacts remains every day, are faced with the blood that is had the disease carrying germ remains, the danger that body fluid infects.Standard GB 19053-2003 has stipulated all kinds of funerals place pathogenic bacteria safety limit, requires remains in time to sterilize, between hearse, morgue, lift face, take leave of the Room, sterilization regularly such as between cremating.Be directed to infectious disease remains such as " SARS ", " Influenza A H1N1 ", and the disaster area sterilization, people generally use 1000～2000mg/L available chlorine chlorine-containing disinfectant or 0.5% peracetic acid soln disinfection.But chlorine-containing disinfectant has tangible spread effect to people's respiratory mucosa and skin, and severe patient causes chlorine poisoning; Metal and native fiber textile are had corrosiveness, pigment is had discoloration, a large amount of uses also pollute the environment; And Peracetic acid is inflammable, and tool explosivity, strong oxidizing property, severe corrosive, strong and stimulating can cause human body and burn.Therefore; For remedying existing disinfectant to environment and the big shortcoming of human health damage; Be badly in need of that research and development are efficient, low toxicity, environmental protection, and meet the funeral and interment appropriative disinfector of funeral place health characteristics, with protection funeral and interment staff healthy, create the comfortable working environment of health.
Disinfectant is meant the chemicals that is used for killing the external environment pathogenic microorganism.Can be divided into bactericidal agent, high effect disinfectants, middle effect disinfectants, low effect disinfectants according to its exposure level.Bactericidal agent is meant can kill the preparation of (comprising bacterial propagule, gemma, fungi, virus, Richettsia etc.) of all microorganisms in the external environment, comprises formaldehyde, glutaraldehyde, oxirane, Peracetic acid, hydrogen peroxide, chlorine dioxide etc.High effect disinfectants can be killed various microorganisms, and disinfectant that also can the kill bacteria brood cell when using enough concentration comprises chlorine-containing disinfectant, ozone, double-chain quaternary ammonium salt etc.Middle effect disinfectants can not the kill bacteria brood cell, comprises containing iodine disinfectant, alcohol disinfectant, phenols disinfectant etc.But low effect disinfectants refers to only kill vegetative forms of bacteria and close nma virus, comprises two guanidine class disinfectant and Chinese herbal disinfectants such as quaternary ammonium disinfectant, Chlorhexidine such as benzalkonium bromide.(list of references 1: Yang Huaming, Yi Bin. modern hospital Disinfection, People's Medical Officer Press; List of references 2: Xue Guangbo. sterilization antiseptic preservation, People's Health Publisher.)
(o-Phthalaldehyde OPA) is a kind of novel high effect disinfectants that abroad in recent years works out to OPA, and 1994 begin to be used for sterilizing endoscope, and have passed through the U.S. FDA authentication.Research shows that it is fast, effective that OPA kills speed to bacterial propagule, virus and mycobacterium; Mycobacterium killing effect to anti-glutaraldehyde and Peracetic acid is good; Low to people's toxicity, have no irritating odor, article are not had advantages such as damage effect (list of references 3: Hou Tieying, Huang Dehong. the research of the sterilization effect of OPA, Chinese hospital infection magazine, 2005,15 (6): 654～656.), application prospect is extensive, might become the substitute of glutaraldehyde.Benzalkonium bromide (bromogeramine) is the strand quaternary ammonium disinfectant, has sterilization and de-sludging concurrently and renders a service, and has nontoxic, nonirritant, and fungicidal spectrum is wide, kill characteristics such as the reproduction type bacterial action is strong, safe in utilization.The inventor has carried out experimental study repeatedly, in the solution system that with OPA, benzalkonium bromide is main component, adds alcohol, surfactant etc., the remains that preparation obtains a kind of efficient, low toxicity, the environmental protection compound preparation of sterilizing.
Summary of the invention
The objective of the invention is to overcome the deficiency of existing disinfectant; Providing a kind of is efficient, the low toxicity of main component, remains disinfectant of environmental protection and preparation method thereof by OPA, benzalkonium bromide; Being applied to remains and relevant environment thereof, and the sterilization of air, general body surface.
The present invention relates to remains disinfectant of a kind of efficient, low toxicity, environmental protection and preparation method thereof, this disinfectant is that 0.4～0.6% OPA, 0.05～0.1% benzalkonium bromide, 0.2～0.5% polyoxyethylene laurel ether, 2～10% ethanol, 1～3% isopropyl alcohol, 0.25～1.5% organic acid, 0～0.3% essence and 84～96% distilled water constitute by mass percent mainly.Prove through stability test, microorganism killing test (containing the neutralizer approval test), the funeral parlor on-the-spot sterilization approval test; Disinfectant of the present invention has good stability, germicidal efficiency is high, non-stimulated and advantage such as corrosivity; Can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.
The objective of the invention is to realize like this:
A kind of remains disinfectant provided by the invention and preparation method thereof is characterized in that:
Remains disinfectant of the present invention is that 0.4～0.6% OPA, 0.05～0.1% benzalkonium bromide, 0.2～0.5% polyoxyethylene laurel ether, 2～10% ethanol, 1～3% isopropyl alcohol, 0.25～1.5% organic acid, 0～0.3% essence and 84～96% distilled water constitute by mass percent mainly.
Organic acid in the remains disinfectant of the present invention is selected from acetate and propionic acid, flavoring essences such as the preferred pineapple of essence, orange.
The preparation method of remains disinfectant of the present invention comprises the steps: by the prescription ratio, and OPA is soluble in water, adds benzalkonium bromide, polyoxyethylene laurel ether successively, stirs, fully dissolving; Add ethanol, isopropyl alcohol, organic acid and essence then while stirring, mixing promptly gets.
Remains disinfectant of the present invention can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.It is antirust to add 0.5% natrium nitrosum when using remains disinfectant of the present invention to sterilize metallic weapon.
Remains disinfectant provided by the invention and method for using have following advantage:
1. broad spectrum activity: OPA all has very strong killing action to bacterial propagule, fungi, mycobacterium, virus, gemma even some parasite, especially glutaraldehyde resistance mycobacterium tuberculosis strain is also had good killing action; The benzalkonium bromide fungicidal spectrum is wide, and suppurative pathogen is had good killing action, and the killing action of gram positive bacteria is greater than negative bacteria.Both are composite, and sterilization is wide spectrum more.
2. high efficiency: OPA is a kind of high effect disinfectants, and is higher with the composite back of benzalkonium bromide germicidal efficiency; Polyoxyethylene laurel ether is a non-ionic surface active agent, has good emulsification, dispersion and unique wettability, can reduce microorganism and organic surface tension, improves bactericidal effect;
3. stability is strong: OPA is more stable under acid condition, and alcohol, non-ionic surface active agent etc. can increase its stability.
4. low toxicity, environmental pollution is little.The skin absorbs toxicity of OPA is lower, and rabbit absorbed the toxicity median lethal dose greater than 2000mg/kg through skin; Acute oral half lethal dose to mouse is 121mg/kg～170mg/kg, is moderate toxicity.Benzalkonium bromide toxicity is very little, and side effect is little, and 0.1% following concentration skin nonirritant can be used for skin, mucous membrane and wound disinfection.
5. easy to use: disinfectant of the present invention and water dissolve each other, and have good penetration power and cleaning action; Contain essence, can remove because the peculiar smell that mould and microbial growth are produced.Its preparation method is easy, and raw material is easy to get, and is workable; Practical application is convenient, is beneficial to and promotes the use of.
In the remains disinfectant component of the present invention, contain OPA, bromogeramine, surfactant, alcohols, spices etc., prescription is reasonable, and ratio is appropriate.Prove through stability test, microorganism killing test (containing the neutralizer approval test), the funeral parlor on-the-spot sterilization approval test; Disinfectant of the present invention has good stability, germicidal efficiency is high, non-stimulated and advantage such as corrosivity; Can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.
Embodiment
Embodiment 1: the neutralizer approval test
Test strain is selected staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922) and Candida albicans (ATCC 10231), establishes parallel 6 groups, carries out the neutralizer approval test by suspension quantitative test program.The result judges: the 1st group of asepsis growth or bacterium number are far fewer than the 2nd group; 2 groups of bacterium numbers obviously are less than 3,4,5 groups; 3rd, 4,5 groups of bacterium numbers are similar, its error rate≤10%; The 6th group of asepsis growth.Show selected neutralizer and concentration thereof for suitable, test repetition 3 times.
Test shows; To contain the phosphate buffer of 4g/L lecithin, 45g/L Tween-80,10g/L glycine; Can effectively neutralize this disinfectant to the residual action of test organisms, and neutralizer and neutralized reaction product thereof have no adverse effects to test organisms and medium, and (seeing table 1) meets the requirements prescribed.
Table 1 disinfectant neutralizer of the present invention is selected result of the test
Annotate: the result is the mean value of 3 tests, and test temperature is 20～21 ℃, and be 10min action time.
Embodiment 2: bacterium (fungi) is quantitatively killed test
For estimating the killing effect of disinfectant of the present invention,, carried out bacteria quantified respectively and killed test and kill test with fungi according to disinfection technology standard (version in 2002) to microorganism.Bacterial classification is selected staphylococcus aureus ATCC6538, Escherichia coli ATCC 25922, Candida albicans ATCC 10231.By the working concentration and the action time of appointment, repeated test 3 times.
The result shows (table 2), and when using 300 times of disinfectant of the present invention dilutions, action time to be 3min, suspension is quantitatively killed test, to staphylococcus aureus, Escherichia coli, each time of Candida albicans test kill logarithm value all >=5.00, be judged to be sterilize qualified.
Table 2 disinfectant of the present invention is to the killing effect of bacterium (fungi)
Annotate: test temperature is 20～21 ℃, positive control bacterium number: staphylococcus aureus is 4.5 * 10
4CFU/mL, Escherichia coli are 3.2 * 10
4CFU/mL, Candida albicans are 1 * 10
4CFU/mL, result are 3 test mean values.
Embodiment 3: stability test
Accelerated test: raw sample is put 14d in 54 ℃～56 ℃ insulating boxs, keep relative moisture >=75%, sampling is carried out antibacterial or the bactericidal property test.
Experimental result shows (table 3), uses 200 times of disinfectant dilutions of the present invention, action time to be can reach 100% to the quantitative killing rate of the suspension of staphylococcus aureus by 3min; Use 400 times of disinfectant of the present invention dilutions, action time 3min, all can reach 100% the average kill ratio of Escherichia coli and Candida albicans.54 ℃ of accelerated test explanations, its sterilizing rate or bacteriostasis rate can reach the specified standard value, and the sterilization of product or the bacteriostasis term of validity are room temperature preservation at least 1 year.Explain that disinfectant stability of the present invention is strong.
Table 3 disinfectant stability test of the present invention result
Annotate: test temperature is 20～21 ℃, positive control bacterium number: staphylococcus aureus is 4.5 * 10
4CFU/mL, Escherichia coli are 3.2 * 10
4CFU/mL, Candida albicans are 0.84 * 10
4CFU/mL more than is 2 result of the tests.
Embodiment 4: the field experiment of the disinfectant funeral parlor
In the funeral parlor, randomly draw part remains and body surface (lift face instrument, door handle, stretcher handle, refrigerating box handle etc.) sampling, agent field experiment carries out disinfection.Wipe away with the aseptic cotton that is soaked with Plondrel hydrochlorate (PBS) buffer solution during sampling, various body surfaces are smeared sampling by the regulation area respectively, disinfecting time is made as 3min, and the sampling cotton is wiped away aseptic cutting in the sterile test tube that 10mL PBS buffer solution is housed.Through the wash-out that fully vibrates, get eluent with agar tilt-pour process inoculation plate, 3 plates of each sample inoculation are put in 37 ℃ of insulating boxs and are cultivated 48h, observe the colony growth situation.
Field experiment result in the disinfectant funeral parlor sees table 4.Disinfectant of the present invention is 99.53% to the average kill ratio of remains surface bacteria, is 91.86% to the average kill ratio of body surface bacterium, the sterilization sample on average kill logarithm value >=1, it is qualified to be judged to sterilization.Explain that disinfectant germicidal efficiency of the present invention is high, practical application effect is good, has very high promotional value.
Table 4 disinfectant of the present invention funeral parlor field experiment result
Claims (6)
1. remains disinfectant is characterized in that this disinfectant is that 0.4～0.6% OPA, 0.05～0.1% benzalkonium bromide, 0.2～0.5% polyoxyethylene laurel ether, 2～10% ethanol, 1～3% isopropyl alcohol, 0.25～1.5% organic acid, 0～0.3% essence and 84～96% distilled water constitute by mass percent mainly.
2. remains disinfectant according to claim 1 is characterized in that by OPA, benzalkonium bromide be the compound preparation of main component.
3. disinfectant according to claim 1 is characterized in that described organic acid is selected from acetate and propionic acid, flavoring essences such as the preferred pineapple of essence, orange.
4. the preparation method of disinfectant according to claim 1 is characterized in that the preparation method of this disinfectant comprises the steps:
By the prescription ratio, OPA is soluble in water, add benzalkonium bromide, polyoxyethylene laurel ether successively, stir, fully dissolving; Add ethanol, isopropyl alcohol, organic acid and essence then while stirring, mixing promptly gets.
5. disinfectant according to claim 1, it is antirust to add 0.5% natrium nitrosum when it is characterized in that sterilizing metallic weapon.
6. disinfectant according to claim 1 is characterized in that can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010546662.0A CN102461499B (en) | 2010-11-17 | 2010-11-17 | Disinfector for remains and preparation method thereof |
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| CN201010546662.0A CN102461499B (en) | 2010-11-17 | 2010-11-17 | Disinfector for remains and preparation method thereof |
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| CN102461499A true CN102461499A (en) | 2012-05-23 |
| CN102461499B CN102461499B (en) | 2014-06-18 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104094923A (en) * | 2013-04-09 | 2014-10-15 | 民政部一零一研究所 | Preservative liquid for corpses, tissue and dissections, and application method thereof |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
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| CN1613308A (en) * | 2004-09-24 | 2005-05-11 | 北京长江脉医药科技有限责任公司 | Antiseptics and preparation thereof |
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Patent Citations (5)
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| CN1565635A (en) * | 2003-06-21 | 2005-01-19 | 李君� | Chemical disinfectant with broad spectrum sterilization |
| CN1613308A (en) * | 2004-09-24 | 2005-05-11 | 北京长江脉医药科技有限责任公司 | Antiseptics and preparation thereof |
| CN1836508A (en) * | 2005-03-25 | 2006-09-27 | 黄雅钦 | Novel compound o-Phthalaldehyde microemusion sterilization composition |
| CN100998880A (en) * | 2006-08-28 | 2007-07-18 | 广东环凯微生物科技有限公司 | O-phthalic aldehyde antiseptic sterilization agent and its preparation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104094923A (en) * | 2013-04-09 | 2014-10-15 | 民政部一零一研究所 | Preservative liquid for corpses, tissue and dissections, and application method thereof |
| CN104094923B (en) * | 2013-04-09 | 2016-06-15 | 民政部一零一研究所 | A kind of antiseptic preserving fluid for corpse, tissue and dissection and using method thereof |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
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| CN102461499B (en) | 2014-06-18 |
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