CN102461499B - Disinfector for remains and preparation method thereof - Google Patents
Disinfector for remains and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a high-efficient, low-toxicity and environmental-friendly disinfector for remains and a preparation method thereof. The disinfector comprises the following components in percentage by mass: 0.4-0.6 percent of o-phthaldialdehyde, 0.05-0.1 percent of benzalkonium bromide, 0.2-0.5 percent of polyethylene glycol monooleyl ether, 2-10 percent of ethanol, 1-3 percent of isopropanol, 0.25-1.5 percent of organic acid, 0-0.3 percent of essence and 84-96 percent of distilled water. As demonstrated by a stability test, a disinfection microorganism test (including a neutralizer appraisal test) and a funeral parlour field disinfection trial, the disinfector provided by the invention has the advantages of good stability, high sterilization efficiency, no stimulation or corrosivity, etc., and can be used for disinfecting the remains, relevant environments, air and general object surfaces.
Description
Technical field
The present invention relates to a kind of chemosterilant, be specifically related to remains disinfectant of a kind of high-efficiency low-toxicity that can be applicable to corpse and relevant environment thereof and preparation method thereof.
Background technology
Funeral and interment place is a special public place, and remains are main pollution source.The various pathogenic microorganisms that remains carry, as saprophytic bacteria and (condition) pathogenic bacteria etc., can be spread out in each process of funeral service, cause the microbial contaminations such as air in environment, body surface, remains surface, worker's watch face.Remains connect fortune work, anticorrosion lift face work, explosive industry all directly contacts remains every day, are faced with the danger of being infected by the blood with disease carrying germ remains, body fluid.Standard GB 19053-2003 has specified all kinds of funerals place pathogenic bacteria safety limit, requires remains to sterilize in time, between hearse, morgue, lift face, take leave of the Room, the regularly sterilization such as between cremating.Be directed to the infectious disease remains such as " SARS (Severe Acute Respiratory Syndrome) ", " Influenza A H1N1 ", and disaster area sterilization, people generally use 1000～2000mg/L available chlorine chlorine-containing disinfectant or 0.5% peracetic acid soln disinfection.But respiratory mucosa and the skin of chlorine-containing disinfectant to people has obvious spread effect, and severe patient causes chlorine poisoning; Metal and Nature Fibre Textile product are had to corrosiveness, pigment is had to discoloration, a large amount of uses also pollute the environment; And Peracetic acid is inflammable, tool explosivity, strong oxidizing property, severe corrosive, strong and stimulating, can cause human body and burn.Therefore; for making up existing disinfectant to environment and the large shortcoming of human health damage; be badly in need of that research and development are efficient, low toxicity, environmental protection, and meet the funeral and interment appropriative disinfector of funeral place health feature, to protect funeral and interment staff's healthy, the working environment of creating sanitory.
Disinfectant refers to the chemicals for killing external environment pathogenic microorganism.Can be divided into bactericidal agent, high effect disinfectants, middle effect disinfectants, low effect disinfectants according to its exposure level.Bactericidal agent refers to can kill the preparation of (comprising bacterial propagule, gemma, fungi, virus, Richettsia etc.) of all microorganisms in external environment, comprises formaldehyde, glutaraldehyde, oxirane, Peracetic acid, hydrogen peroxide, chlorine dioxide etc.High effect disinfectants can be killed various microorganisms, and disinfectant that also can killing bacterial spores while using enough concentration, comprises chlorine-containing disinfectant, ozone, double-chain quaternary ammonium salt etc.Middle effect disinfectants can not killing bacterial spores, comprises containing iodine disinfectant, alcohol disinfectant, phenols disinfectant etc.Low effect disinfectants only refers to can kill vegetative forms of bacteria and close nma virus, comprises quaternary ammonium disinfectant, the Chlorhexidines etc. such as benzalkonium bromide two guanidine class disinfectant and Chinese herbal disinfectants.(bibliography 1: Yang Huaming, Yi Bin. modern hospital Disinfection, People's Medical Officer Press; Bibliography 2: Xue Guangbo. sterilization antiseptic preservation, People's Health Publisher.)
O-phthalaldehyde(OPA) (o-Phthalaldehyde, OPA) is a kind of novel high effect disinfectants that external Recent study goes out, and 1994 start for sterilizing endoscope, and has passed through U.S. FDA certification.Research shows, it is fast, effective that OPA kills speed to bacterial propagule, virus and mycobacterium; Mycobacterium killing effect to resistance to glutaraldehyde and Peracetic acid is good; Low to people's toxicity, have no irritating odor, to article without the advantages such as damage effect (bibliography 3: Hou Tieying, Huang Dehong. the sterilization effect research of o-phthalaldehyde(OPA), Chinese Journal of Nosocomiology, 2005,15 (6): 654～656.), application prospect is extensive, likely becomes the substitute of glutaraldehyde.Benzalkonium bromide (bromogeramine), is strand quaternary ammonium disinfectant, has sterilization and de-sludging effect concurrently, has nontoxic, nonirritant, fungicidal spectrum is wide, kill reproduction type bacterial action strong, use the features such as safety.The inventor has carried out experimental study repeatedly, and adding alcohol, surfactant etc. taking o-phthalaldehyde(OPA), benzalkonium bromide in the solution system of main component, preparation obtains the remains sterilization compound preparation of a kind of efficient, low toxicity, environmental protection.
Summary of the invention
The object of the invention is to overcome the deficiency of existing disinfectant, provide that a kind of to be main component by o-phthalaldehyde(OPA), benzalkonium bromide efficient, the remains disinfectant of low toxicity, environmental protection and preparation method thereof, to be applied to remains and relevant environment thereof, and the sterilization of air, general body surface.
The present invention relates to remains disinfectant of a kind of efficient, low toxicity, environmental protection and preparation method thereof, the o-phthalaldehyde(OPA) that this disinfectant is mainly 0.4～0.6% by mass percent, 0.05～0.1% benzalkonium bromide, 0.2～0.5% polyoxyethylene laurel ether, 2～10% ethanol, 1～3% isopropyl alcohol, 0.25～1.5% organic acid, 0～0.3% essence and 84～96% distilled water form.Prove through stability test, Germicidal test (containing neutralizer approval test), the funeral parlor on-the-spot sterilization approval test, disinfectant of the present invention has the advantages such as high, the non-stimulated and corrosivity of good stability, germicidal efficiency, can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.
The object of the present invention is achieved like this:
A kind of remains disinfectant provided by the invention and preparation method thereof, is characterized in that:
Remains disinfectant of the present invention, the o-phthalaldehyde(OPA) that is mainly 0.4～0.6% by mass percent, 0.05～0.1% benzalkonium bromide, 0.2～0.5% polyoxyethylene laurel ether, 2～10% ethanol, 1～3% isopropyl alcohol, 0.25～1.5% organic acid, 0～0.3% essence and 84～96% distilled water form.
Organic acid in remains disinfectant of the present invention is selected from acetic acid and propionic acid, the flavoring essences such as the preferred pineapple of essence, orange.
The preparation method of remains disinfectant of the present invention comprises the steps: by recipe ratio, and o-phthalaldehyde(OPA) is soluble in water, adds successively benzalkonium bromide, polyoxyethylene laurel ether, stirs, and fully dissolves; Then add while stirring ethanol, isopropyl alcohol, organic acid and essence, mix and get final product.
Remains disinfectant of the present invention can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.While using remains disinfectant sterilization metallic weapon of the present invention, need add 0.5% natrium nitrosum antirust.
Remains disinfectant provided by the invention and using method, have following advantage:
1. broad spectrum activity: o-phthalaldehyde(OPA) to bacterial propagule, fungi, mycobacterium, virus, gemma even some parasite have very strong killing action, especially to glutaraldehyde Resistance Mycobacterium Tuberculosis, strain also has good killing action; Benzalkonium bromide fungicidal spectrum is wide, and suppurative pathogen is had to good killing action, and the killing action of gram positive bacteria is greater than to negative bacteria.Both are composite, and sterilization is wide spectrum more.
2. high efficiency: o-phthalaldehyde(OPA) is a kind of high effect disinfectants is higher with the composite rear germicidal efficiency of benzalkonium bromide; Polyoxyethylene laurel ether is non-ionic surface active agent, has good emulsification, dispersion and unique wettability, can reduce microorganism and organic surface tension, improves bactericidal effect;
3. stability is strong: o-phthalaldehyde(OPA) is more stable under acid condition, and alcohol, non-ionic surface active agent etc. can increase its stability.
4. low toxicity, environmental pollution is little.It is lower that the skin of o-phthalaldehyde(OPA) absorbs toxicity, absorbing toxicity median lethal dose through skin and be greater than 2000mg/kg rabbit; Being 121mg/kg～170mg/kg to the acute oral half lethal dose of mouse, is moderate toxicity.Benzalkonium bromide toxicity is very micro-, and side effect is little, and 0.1% following concentration skin nonirritant, can be used for skin, mucous membrane and wound disinfection.
5. easy to use: disinfectant of the present invention and water dissolve each other, there is good penetration power and cleaning action; Contain essence, can remove the peculiar smell producing due to mould and microbial growth.Its preparation method is easy, and raw material is easy to get, workable; Practical application is convenient, is beneficial to and promotes the use of.
In remains disinfectant component of the present invention, contain o-phthalaldehyde(OPA), bromogeramine, surfactant, alcohols, spices etc., rationally, ratio is appropriate for formula.Prove through stability test, Germicidal test (containing neutralizer approval test), the funeral parlor on-the-spot sterilization approval test, disinfectant of the present invention has the advantages such as high, the non-stimulated and corrosivity of good stability, germicidal efficiency, can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.
Embodiment
Embodiment 1: neutralizer approval test
Test strain is selected staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922) and Candida albicans (ATCC 10231), establishes parallel 6 groups, carries out neutralizer approval test by suspension quantitative test program.Result is judged: the 1st group of asepsis growth or bacterium number are far fewer than the 2nd group; 2 groups of bacterium numbers are obviously less than 3,4,5 groups; 3rd, 4,5 groups of bacterium numbers are similar, its error rate≤10%; The 6th group of asepsis growth.Show that selected neutralizer and concentration thereof are suitable, test repeats 3 times.
Test shows, with the phosphate buffer containing 4g/L lecithin, 45g/L Tween-80,10g/L glycine, can effectively neutralize the residual action of this disinfectant to test organisms, neutralizer and neutralized reaction product thereof have no adverse effects to test organisms and medium, and (in table 1) meets the requirements prescribed.
Table 1 disinfectant neutralizer of the present invention is selected result of the test
Note: result is the mean value of 3 tests, and test temperature is 20～21 DEG C, and be 10min action time.
Embodiment 2: bacterium (fungi) is quantitatively killed test
For evaluating the killing effect of disinfectant of the present invention to microorganism, according to disinfection technology standard (version in 2002), carry out respectively Guantitative bactericidal test and fungi and killed test.Bacterial classification is selected staphylococcus aureus ATCC6538, Escherichia coli ATCC 25922, Candida albicans ATCC 10231.By working concentration and the action time of specifying, repeated test 3 times.
Result shows (table 2), be 3min when using 300 times of disinfectant dilutions of the present invention, action time, suspension is quantitatively killed test, and what staphylococcus aureus, Escherichia coli, Candida albicans were tested for each time kills logarithm value all >=5.00, is judged to be to sterilize qualified.
The killing effect of table 2 disinfectant of the present invention to bacterium (fungi)
Note: test temperature is 20～21 DEG C, positive control bacterium number: staphylococcus aureus is 4.5 × 10
4cFU/mL, Escherichia coli are 3.2 × 10
4cFU/mL, Candida albicans are 1 × 10
4cFU/mL, result is 3 test mean values.
Embodiment 3: stability test
Accelerated test: raw sample is put to 14d in 54 DEG C～56 DEG C insulating boxs, keep relative moisture >=75%, antibacterial or bactericidal property test is carried out in sampling.
Experimental result shows (table 3), and using 200 times of disinfectant dilutions of the present invention, action time is 3min, can reach 100% to the quantitative killing rate of the suspension of staphylococcus aureus; Use 400 times of disinfectant of the present invention dilutions, action time 3min, all can reach 100% to the average kill ratio of Escherichia coli and Candida albicans.54 DEG C of accelerated test explanations, its sterilizing rate or bacteriostasis rate can reach specified standard value, and the sterilization of product or the bacteriostasis term of validity are room temperature preservation at least 1 year.Illustrate that disinfectant stability of the present invention is strong.
Table 3 disinfectant stability test of the present invention result
Note: test temperature is 20～21 DEG C, positive control bacterium number: staphylococcus aureus is 4.5 × 10
4cFU/mL, Escherichia coli are 3.2 × 10
4cFU/mL, Candida albicans are 0.84 × 10
4cFU/mL is more than 2 result of the tests.
Embodiment 4: disinfectant funeral parlor field experiment
In the funeral parlor, randomly draw part remains and body surface (lift face instrument, door handle, stretcher handle, refrigerating box handle etc.) sampling, agent field experiment carries out disinfection.When sampling, wipe with the sterile cotton that is soaked with Plondrel hydrochlorate (PBS) buffer solution, to various body surfaces respectively in accordance with regulations area carry out swabing sampling, disinfecting time is made as 3min, and sampling cotton is wiped to aseptic cutting in the sterile test tube that 10mL PBS buffer solution is housed.Through the wash-out that fully vibrates, get eluent with agar tilt-pour process inoculation plate, 3 plates of each sample inoculation, put in 37 DEG C of insulating boxs and cultivate 48h, observe colony growth situation.
The field experiment of the disinfectant funeral parlor the results are shown in Table 4.Disinfectant of the present invention is 99.53% to the average kill ratio of remains surface bacteria, is 91.86% to the average kill ratio of body surface bacterium, sterilization sample on average kill logarithm value >=1, can be judged to sterilization qualified.Illustrate that disinfectant germicidal efficiency of the present invention is high, practical application effect is good, has very high promotional value.
Table 4 disinfectant of the present invention funeral parlor field experiment result
Claims (6)
1. a remains disinfectant, is characterized in that o-phthalaldehyde(OPA) that this disinfectant is mainly 0.4～0.6% by mass percent, 0.05～0.1% benzalkonium bromide, 0.2～0.5% polyoxyethylene laurel ether, 2～10% ethanol, 1～3% isopropyl alcohol, 0.25～1.5% organic acid, 0～0.3% essence and 84～96% distilled water form;
Described remains disinfectant working concentration and action time: dilute 300 times, be 3min action time.
2. remains disinfectant according to claim 1, the compound preparation that to it is characterized in that by o-phthalaldehyde(OPA), benzalkonium bromide be main component.
3. disinfectant according to claim 1, is characterized in that described organic acid is selected from acetic acid and propionic acid, the preferred pineapple of essence, orange flavoring essence.
4. the preparation method of disinfectant according to claim 1, is characterized in that the preparation method of this disinfectant comprises the steps:
By recipe ratio, o-phthalaldehyde(OPA) is soluble in water, add successively benzalkonium bromide, polyoxyethylene laurel ether, stir, fully dissolve; Then add while stirring ethanol, isopropyl alcohol, organic acid and essence, mix and get final product.
5. disinfectant according to claim 1, need add 0.5% natrium nitrosum while it is characterized in that sterilizing metallic weapon antirust.
6. disinfectant according to claim 1, is characterized in that can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.
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| CN201010546662.0A CN102461499B (en) | 2010-11-17 | 2010-11-17 | Disinfector for remains and preparation method thereof |
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| CN201010546662.0A CN102461499B (en) | 2010-11-17 | 2010-11-17 | Disinfector for remains and preparation method thereof |
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| CN102461499A CN102461499A (en) | 2012-05-23 |
| CN102461499B true CN102461499B (en) | 2014-06-18 |
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| CN104094923B (en) * | 2013-04-09 | 2016-06-15 | 民政部一零一研究所 | A kind of antiseptic preserving fluid for corpse, tissue and dissection and using method thereof |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
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| CN1565635A (en) * | 2003-06-21 | 2005-01-19 | 李君� | Chemical disinfectant with broad spectrum sterilization |
| CN1613308A (en) * | 2004-09-24 | 2005-05-11 | 北京长江脉医药科技有限责任公司 | Antiseptics and preparation thereof |
| CN1836508A (en) * | 2005-03-25 | 2006-09-27 | 黄雅钦 | Novel compound o-Phthalaldehyde microemusion sterilization composition |
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| CN102077828A (en) * | 2009-11-27 | 2011-06-01 | 天津瑞普生物技术股份有限公司 | Compound disinfectant containing quaternary ammonium salt |
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Patent Citations (5)
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| CN1565635A (en) * | 2003-06-21 | 2005-01-19 | 李君� | Chemical disinfectant with broad spectrum sterilization |
| CN1613308A (en) * | 2004-09-24 | 2005-05-11 | 北京长江脉医药科技有限责任公司 | Antiseptics and preparation thereof |
| CN1836508A (en) * | 2005-03-25 | 2006-09-27 | 黄雅钦 | Novel compound o-Phthalaldehyde microemusion sterilization composition |
| CN100998880A (en) * | 2006-08-28 | 2007-07-18 | 广东环凯微生物科技有限公司 | O-phthalic aldehyde antiseptic sterilization agent and its preparation method |
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