CN102648710B - Compound glutaraldehyde disinfectant for body of dead person - Google Patents
Compound glutaraldehyde disinfectant for body of dead person Download PDFInfo
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- CN102648710B CN102648710B CN201110043103.2A CN201110043103A CN102648710B CN 102648710 B CN102648710 B CN 102648710B CN 201110043103 A CN201110043103 A CN 201110043103A CN 102648710 B CN102648710 B CN 102648710B
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Abstract
The invention provides a compound glutaraldehyde disinfectant for a body of a dead person, which mainly consists of the following ingredients by mass percent: 0.5 to 1.5 percent of glutaraldehyde, 0.2 to 0.5 percent of didodecyl dimethyl ammonium bromide, 0.2 to 0.5 percent of polyethylene glycol monooleyl ether, 0.5 to 1.5 percent of organic acid, 0 to 0.5 percent of essence and 95.5 to 98.6 percent of distilled water. The disinfectant is proved to have advantages of good stability, high sterilizing efficiency, no irritation and the like through a stability test, a microorganism killing test (including the neutralizer discrimination test) and on-site sterilization test in funeral parlour, is a high-efficient environment-friendly disinfectant, can be used for sterilizing the body of the dead person and associated environments as well as for sterilizing air and the surface of an ordinary object.
Description
Technical field
The present invention relates to a kind of chemosterilant, be specifically related to a kind of complex glutaraldehyde disinfectant of the high-efficiency environment friendly that can be applicable to remains and relevant environment thereof.
Background technology
Remains are as the important pollution source in funeral and interment place, the various pathogenic microorganisms that carry, as saprophytic bacteria and (condition) pathogenic bacteria etc., can be spread out in each process of funeral service, cause the microbial contaminations such as environmental air, body surface, remains surface, worker's watch face, bring potential hazard to the health at funeral worker and funeral family.Standard GB 19053-2003 has specified all kinds of funerals place pathogenic bacteria safety limit, requires remains to sterilize in time, between hearse, morgue, lift face, take leave of the Room, the regularly sterilization such as between cremating.To the infectious disease remains such as " SARS (Severe Acute Respiratory Syndrome) ", " Influenza A H1N1 ", and disaster area sterilization, people generally use chlorine-containing disinfectant or peracetic acid soln disinfection.But respiratory mucosa and the skin of chlorine-containing disinfectant to people has obvious spread effect, and severe patient causes chlorine poisoning; Metal and Nature Fibre Textile product are had to corrosiveness, pigment is had to discoloration, a large amount of uses also pollute the environment; And Peracetic acid is inflammable, tool explosivity, strong oxidizing property, severe corrosive, strong and stimulating, can cause human body and burn.Therefore; for making up existing disinfectant to environment and the large shortcoming of human health damage; be badly in need of exploring development and be suitable for efficient, the low toxicity of funeral profession, the remains disinfectant of environmental protection, significant to the working environment healthy, creation sanitory at protection funeral worker and vast funeral family.
Disinfectant refers to the chemicals for killing external environment pathogenic microorganism.Common disinfectants mainly comprises: bactericidal agent (as, formaldehyde, glutaraldehyde, oxirane, Peracetic acid, hydrogen peroxide, chlorine dioxide), high effect disinfectants (as, chlorine-containing disinfectant, ozone, double-chain quaternary ammonium salt), middle effect disinfectants (containing iodine disinfectant, alcohol disinfectant, phenols disinfectant), low effect disinfectants (as, benzalkonium bromide, Chlorhexidine, Chinese herbal disinfectant) etc. (bibliography 1: Yang Huaming, Yi Bin. modern hospital Disinfection, People's Medical Officer Press).
Disinfectant is of a great variety in the market, and high-efficiency low-toxicity less is specifically designed to remains and sterilizes still less.Wherein, glutaraldehyde (Glutaraldehyde) is a kind of good bactericidal agent, there is the advantages such as wide spectrum, efficient, little to metal protection, aqueous stability is strong, it is after formaldehyde and oxirane that glutaraldehyde is described as, third generation chemosterilant (bibliography 2: Xue Guangbo. sterilization antiseptic preservation, People's Health Publisher).Current, 2% alkaline glutaric dialdehyde disinfectant products of two-component packaging is the most common, and its stability is low; Developed again afterwards strong acidic glutaraldehyde, what both kept glutaraldehyde kills gemma activity, has again stronger stability.Didodecyldimethylammbromide bromide (Didodecyl Dimethyl Ammonium Bromide, DDDMAB) be a kind of double-chain quaternary ammonium salt, 1 hydrophilic group of water-soluble rear generation and 2 lipophilic groups, adsorbable in microorganism surface, change the permeability of cell membrane, suppress or the structural proteins activity such as destructive enzyme simultaneously, and the microbial death that arrives, belong to high effect disinfectants, DDDMAB is widely used as catalyzer, emulsifier, disinfectant, bactericide, antistatic additive etc.Research in recent years shows: by glutaraldehyde and the composite complex glutaraldehyde disinfectant of making of cationic surfactant (double-chain quaternary ammonium salt), be a kind of composite disinfection agent of high-efficiency low-toxicity wide spectrum according to synergy principle.The inventor has carried out experimental study repeatedly, and preparation obtains that a kind of to be main component by glutaraldehyde, didodecyldimethylammbromide bromide, polyoxyethylene laurel ether efficient, the novel remains sterilization compound preparation of low toxicity, environmental protection.
Summary of the invention
The object of the invention is to overcome the deficiency of existing disinfectant, provide that a kind of to be main component by glutaraldehyde, didodecyldimethylammbromide bromide efficient, the remains disinfectant of low toxicity, environmental protection and preparation method thereof, to be applied to remains and relevant environment thereof, and the sterilization of air, general body surface.
The present invention relates to a kind of compound glutaraldehyde remains disinfectant of efficient, environmental protection, glutaraldehyde that this disinfectant is mainly 0.5～1.5% by mass percent, 0.2～0.5% didodecyldimethylammbromide bromide, 0.2～0.5% polyoxyethylene laurel ether, 0.5～1.5% organic acid, 0～0.5% essence and 95.5～98.6% distilled water form.Prove through stability test, Germicidal test (containing neutralizer approval test), the funeral parlor on-the-spot sterilization approval test, disinfectant of the present invention has the advantages such as good stability, germicidal efficiency are high, nonirritant, can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.
The object of the present invention is achieved like this:
A kind of compound glutaraldehyde remains disinfectant provided by the invention, is characterized in that:
Glutaraldehyde that remains disinfectant of the present invention is mainly 0.5～1.5% by mass percent, 0.2～0.5% didodecyldimethylammbromide bromide, 0.2～0.5% polyoxyethylene laurel ether, 0.5～1.5% organic acid, 0～0.5% essence and 95.5～98.6% distilled water form.Organic acid in remains disinfectant of the present invention is selected from acetic acid and propionic acid, the flavoring essences such as the preferred pineapple of essence, orange, lemon.
The preparation method of remains disinfectant of the present invention comprises the steps: by recipe ratio, and polyoxyethylene laurel ether is soluble in water, adds didodecyldimethylammbromide bromide, stirs, and fully dissolves; Then add while stirring glutaraldehyde, organic acid and essence, mix, canned.
Remains disinfectant of the present invention can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.
Remains disinfectant provided by the invention and using method, have following advantage:
1. broad spectrum activity: glutaraldehyde is a kind of bactericidal agent, can kill and comprise bacterial spore, fungal spore, mycobacterium, and virus and bacterial propagule are in all interior microorganisms.The present invention is strong acidic glutaraldehyde compound disinfectant, in glutaraldehyde, adds a kind of hardening agent---polyoxyethylene laurel ether, and like this, what both kept glutaraldehyde kills gemma activity, has strengthened again stability.Didodecyldimethylammbromide bromide is as a kind of double-chain quaternary ammonium salt, and its bactericidal action is stronger than strand quaternary ammonium salt, and fungicidal spectrum is wider.After glutaraldehyde and didodecyldimethylammbromide bromide are composite, sterilization is wide spectrum more;
2. high efficiency: glutaraldehyde is a kind of good bactericidal agent, and double-chain quaternary ammonium salt belongs to high effect disinfectants, and this compound disinfectant germicidal efficiency is higher; Polyoxyethylene laurel ether is non-ionic surface active agent, has good emulsification, dispersion and unique wettability, can reduce microorganism and organic surface tension, improves bactericidal effect;
3. stability is strong: glutaraldehyde is more stable under acid condition, and double-chain quaternary ammonium salt generally can be fast light, heatproof, good stability, can long term storage;
4. low toxicity, environmental pollution is little: glutaraldehyde has slight excitant and sensitization to skin and mucosa, the oral LD of 2% senaside to small white mouse
50be 298 ± 25mg/kg, in compound disinfectant of the present invention, the working concentration of glutaraldehyde is lower, and toxicity is lower.And it is colourless, tasteless that didodecyldimethylammbromide bromide has, nonirritant, toxicity is little, the conventional sterilization of doing skin and mucosa;
5. easy to use: disinfectant of the present invention and water dissolve each other, there is good penetration power and cleaning action; Contain essence, can remove the peculiar smell producing due to mould and microbial growth.Preparation method is easy, and practical application is convenient, is beneficial to and promotes the use of.
In remains disinfectant component of the present invention, contain glutaraldehyde, double-chain quaternary ammonium salt (didodecyldimethylammbromide bromide), surfactant (polyoxyethylene laurel ether), spices etc., rationally, ratio is appropriate for formula.Prove through stability test, Germicidal test (containing neutralizer approval test), the funeral parlor on-the-spot sterilization approval test, disinfectant of the present invention has the advantages such as good stability, germicidal efficiency be high, non-stimulated, can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.
Embodiment
Embodiment 1: neutralizer approval test
Test strain is selected staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922) and Candida albicans (ATCC 10231), establishes parallel 6 groups, carries out neutralizer approval test by suspension quantitative test program.Result is judged: the 1st group of asepsis growth or bacterium number are far fewer than the 2nd group; 2 groups of bacterium numbers are obviously less than 3,4,5 groups; 3rd, 4,5 groups of bacterium numbers are similar, its error rate≤10%; The 6th group of asepsis growth.Show that selected neutralizer and concentration thereof are suitable, test repeats 3 times.
Test shows, with the PBS solution containing 1% glycine, 1% Tween 80,0.15% lecithin, can effectively neutralize the residual action of this disinfectant to test organisms, neutralizer and neutralized reaction product thereof have no adverse effects to test organisms and medium, meet the requirement (in table 1) that version disinfection technology standard specified in 2002.
Table 1 disinfectant neutralizer of the present invention is selected result of the test
Note: result is the mean value of 3 tests, and test temperature is 20～21 DEG C, and disinfectant of the present invention is respectively to each bacterial strain effect 1min, and the neutralization time is 10min.
Embodiment 2: bacterium (fungi) is quantitatively killed test
For evaluating the killing effect of disinfectant of the present invention to microorganism, according to disinfection technology standard (version in 2002), carry out respectively Guantitative bactericidal test and fungi and killed test.Bacterial classification is selected: the representative of pyococcus in using staphylococcus aureus ATCC 6538 as bacterial propagule, and Escherichia coli ATCC 25922 is as the representative of enteron aisle bacterium in bacterial propagule, and Candida albicans ATCC 10231 is as the representative of pathogenic fungus.By working concentration and the action time of specifying, repeated test 3 times.
Result shows (table 2), in the time using 300 times of disinfectant dilutions of the present invention, action time for 3min, staphylococcus aureus and colibacillary killing rate is reached to 100%, kills logarithm value >=5.00, shows to sterilize qualified; In the time using 100 times of disinfectant dilutions of the present invention, action time for 3min, the killing rate of each test of Candida albicans is reached to 100%, kill logarithm value >=4.00, can judge that this product is qualified to fungal contamination sterilization.
The killing effect of table 2 disinfectant of the present invention to bacterium (fungi)
Note: test temperature is 20～21 DEG C, positive control bacterium number: staphylococcus aureus is 5.3 × 10
4cFU/mL, Escherichia coli are 4.2 × 10
4cFU/mL, Candida albicans are 0.8 × 10
4cFU/mL, result is 3 test mean values.
Embodiment 3: stability test
Accelerated test: raw sample is put to 14d in 54 DEG C～56 DEG C insulating boxs, keep relative moisture >=75%, antibacterial or bactericidal property test is carried out in sampling.
Experimental result shows (table 3), and using 100 times of disinfectant dilutions of the present invention, action time is 3min, can reach 100% to staphylococcus aureus and Escherichia coli average kill ratio, can reach 99.98% to the quantitative killing rate of the suspension of Candida albicans.Explanation thus, after 54 DEG C of accelerated tests, disinfectant of the present invention still can reach the requirement of sterilization to the killing effect of microorganism, can be judged to and pass through.Therefore, disinfectant stability of the present invention is strong, and the sterilization of product or the bacteriostasis term of validity are room temperature preservation at least 1 year.
Table 3 disinfectant stability test of the present invention result
Note: test temperature is 20～21 DEG C, positive control bacterium number: staphylococcus aureus is 8.8 × 10
4cFU/mL, Escherichia coli are 3.3 × 10
4cFU/mL, Candida albicans are 1.5 × 10
4cFU/mL is more than 3 result of the tests.
Embodiment 4: the on-the-spot disinfection experiment in the funeral parlor
In the funeral parlor, Heilongjiang Province, randomly draw part remains and body surface (lift face instrument, door handle, stretcher handle, refrigerating box handle etc.) sampling, carry out disinfectant field experiment of the present invention, select 75% alcohol to test in contrast.When sampling, sterile cotton is wiped in the test tube of phosphate buffer and is bedewed, to each body surface respectively in accordance with regulations area carry out swabing sampling.Then wipe and dip disinfectant with sterile cotton, embrocate sterilization gathering former state position, be 3min action time, wipes sampling with sterile cotton afterwards, and cotton is wiped sampling end and cuts in sterilizing neutralizer test tube.Sampling pipe, through the wash-out that fully vibrates, is got to eluent with agar tilt-pour process inoculation plate, and 3 plates of each sample inoculation, put in 37 DEG C of insulating boxs and cultivate 48h, count plate.
This experiment remains 30 tools (the wherein male sex's 19 tools, women's 11 tools) of sampling altogether, 12 parts, other object.The field experiment of the disinfectant funeral parlor the results are shown in Table 4.Disinfectant of the present invention is 97.94% to the average kill ratio of remains surface bacteria, is 94.71% to the average kill ratio of body surface bacterium, sterilization sample on average kill logarithm value >=1, be significantly better than 75% alcohol control group, can be judged to sterilization qualified.Illustrate that disinfectant germicidal efficiency of the present invention is high, practical application effect is good, has very high promotional value.
Table 4 disinfectant of the present invention funeral parlor field experiment result
Claims (5)
1. a compound glutaraldehyde remains disinfectant, is characterized in that glutaraldehyde that this disinfectant is mainly 0.5～1.5% by mass percent, 0.2～0.5% didodecyldimethylammbromide bromide, 0.2～0.5% polyoxyethylene laurel ether, 0.5～1.5% organic acid, 0～0.5% essence and 95.5～98.6% distilled water form.
Described remains disinfectant working concentration and action time: dilute 100 times, be 3min action time.
2. remains disinfectant according to claim 1, the compound preparation that to it is characterized in that by glutaraldehyde, didodecyldimethylammbromide bromide be main component.
3. disinfectant according to claim 1, is characterized in that described organic acid is selected from acetic acid and propionic acid, the preferred pineapple of essence, orange, lemon flavoring essence.
4. the preparation method of disinfectant according to claim 1, is characterized in that the preparation method of this disinfectant comprises the steps: by recipe ratio, and polyoxyethylene laurel ether is soluble in water, adds didodecyldimethylammbromide bromide, stirs, and fully dissolves; Then add while stirring glutaraldehyde, organic acid and essence, mix, canned.
5. disinfectant according to claim 1, is characterized in that can be used for the sterilization of remains and relevant environment thereof, and the sterilization of air, general body surface.
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| CN104094923B (en) * | 2013-04-09 | 2016-06-15 | 民政部一零一研究所 | A kind of antiseptic preserving fluid for corpse, tissue and dissection and using method thereof |
| CN111420102A (en) * | 2019-01-10 | 2020-07-17 | 广东阜和实业有限公司 | Binary packaged non-combustible air freshener and preparation method thereof |
| CN110339384A (en) * | 2019-07-31 | 2019-10-18 | 山东菏泽三仪生物工程有限公司 | A kind of novel pasture sky house atomizing fumigation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1054521A (en) * | 1990-03-07 | 1991-09-18 | 同济医科大学 | Strengthening glutaric aldehyde disinfectant |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1054521A (en) * | 1990-03-07 | 1991-09-18 | 同济医科大学 | Strengthening glutaric aldehyde disinfectant |
Non-Patent Citations (2)
| Title |
|---|
| 唐冬雁等.溴化双十二烷基季铵盐的合成及缓蚀、杀菌性能研究.《哈尔滨工业大学学报》.2006,第38卷(第4期),538-540，632. * |
| 溴化双十二烷基季铵盐的合成及缓蚀、杀菌性能研究;唐冬雁等;《哈尔滨工业大学学报》;20060430;第38卷(第4期);538-540，632 * |
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