TWI405847B - Aqueous disinfectants and sterilants - Google Patents

Description

Aqueous disinfectant and sterilizing agent

The present invention is directed to consumer safe compositions and their associated uses. The disinfecting composition can be used in a variety of applications, including hard surface cleaning, and can be effectively used as a commercial and/or personal disinfectant or even a sterilizing agent.

Disinfectants and sterilizing agents such as hard surface disinfectants and sterilizing agents are widely used in home and professional devices. Generally, although disinfectants and sterilizing agents are used for the same purpose, such as killing bacteria and/or viruses, the sterilizing composition exhibits a greater "sterilization level" than the disinfectant. That is, most applications require only a disinfectant to reduce the bacteria/virus, although the benefits of other applications are largely due to the use of sterilizing agents. For example, in the pharmaceutical/dental industry, such as floors, walls, countertops, hard surfaces of pharmaceutical/dental instruments and equipment, etc., need to be cleaned or even sterilized for safe patient care.

A common example of a hard surface cleaning product is Lysol. Disinfectant. Although Lysol Effective for many applications, Lysol It is not as effective as the commercially available aqueous solution of glutaraldehyde for reducing the bacterial content. Aqueous glutaraldehyde solutions are widely used as disinfectants (and often sterilizing agents) and are often supplied in solutions of 1% by weight and 2% by weight, especially in pharmaceutical and dental devices. Glutaraldehyde solutions are generally used in more sophisticated pharmaceutical/dental instruments that would otherwise be susceptible to damage by other sterilization methods, such as autoclaving. However, glutaraldehyde is also a strong irritant and respiratory sensitizer. In fact, there have been reports of personal sensibility due to fumigation, which causes breathing problems, headaches, lethargy, skin discoloration, and the like. Because of these issues related to glutaraldehyde fumigation, it is often necessary to monitor air quality or have proper air ventilation. As a result, although the glutaraldehyde solution is a relatively effective disinfectant or even a sterilizing agent, it is desirable to provide a composition that can exhibit even more effective bacterial bactericidal levels while being safer for individuals using the disinfectant/sterilizing agent.

It has been confirmed that it is desirable to provide a liquid solution and a dispersion disinfectant which are effective for cleaning surfaces, particularly hard surfaces. According to this aspect, the aqueous disinfecting or sterilizing composition may comprise an aqueous carrier comprising water, from 0.001% to 50% by weight of peracid, and from 0.001% to 25% by weight of peroxide. In addition, the aqueous disinfectant or sterilizing agent may comprise a transition metal from 0.001 ppm to 50,000 ppm by weight based on the aqueous carrier content, but when the transition metal or alloy is present only in the form of an ionic metal or salt, the composition is substantially Does not contain aldehydes.

In another embodiment, a method of surface disinfection, such as a hard surface. This method can include contacting the surface with a disinfecting composition. The disinfecting composition may comprise an aqueous carrier comprising water, from 0.001% to 50% by weight of peracid, and from 0.001% to 25% by weight of peroxide. Additionally, the aqueous disinfectant or sterilant may comprise from 0.001 ppm to 50,000 ppm by weight of transition metal based on the aqueous carrier content.

Reference is made to specific examples of the examples, and specific language will be used herein to describe them. In any event, it is not intended to limit the scope of the invention. </ RTI> </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; It should also be understood that the words used herein are for the purpose of describing particular embodiments. Unless otherwise specified, the term is not limiting.

It must be noted that the singular forms "a" and "the"

The phrase "food grade" as used in relation to the compositions of the present invention means that the composition is substantially free of ingredients which are considered to be harmful or toxic to mammals when consumed above levels generally considered safe.

The word "solution" is also used throughout the specification to describe the liquid composition of the present invention. However, if these "solutions" include colloidal transition metals, these compositions may also describe component dispersions or suspensions. Since the continuous phase is usually a solution and the transition metal is present as a colloid, these compositions are generally referred to herein as "solutions" for convenience.

The use of the term "substantially free" when considering the disinfecting composition of the present invention means that a particular compound or composition is absent or nearly non-existent. For example, when it is indicated that the composition is substantially free of aldehyde, there is only a trace amount of aldehyde in the composition without aldehyde.

Concentrations, dimensions, amounts, and other numerical data may exist in this form. It is to be understood that the scope of the present invention is to be construed as a limitation of the scope of the The secondary range contained within. For example, a weight ratio range of from about 1% by weight to about 20% by weight should be construed to include not only the clearly indicated limits of 1% by weight and about 20% by weight, but also includes, for example, 2% by weight, 11% by weight, and 14% by weight. Individual weights, and sub-ranges such as 10% to 20% by weight, 5% by weight to 15% by weight, and the like.

According to this aspect, the aqueous disinfecting or sterilizing composition may comprise an aqueous carrier comprising water, from 0.001% to 50% by weight of peracid, and from 0.001% to 25% by weight of peroxide. Additionally, transition metals from 0.001 ppm to 50,000 ppm by weight based on the aqueous carrier content may also be present, but the disinfecting composition is substantially free of aldehydes. In another embodiment, the aqueous disinfecting or sterilizing composition can comprise an aqueous carrier comprising water, from 0.001% to 50% by weight of peracid, and from 0.001% to 25% by weight of peroxide. Moreover, colloidal transition metals from 0.001 ppm to 50,000 ppm by weight, based on the aqueous carrier content, may also be present.

In one embodiment, the aqueous disinfecting or sterilizing composition can include only food grade or food safe ingredients. For example, although not essential, the compositions may be substantially free of disinfecting ingredients that are commonly found in many commercially available surface cleaners. Examples of non-food grade ingredients that may be omitted from the disinfectants or sterilizing agents of the present invention include, but are not limited to, aldehydes such as glutaraldehyde, chlorine based disinfectants, chlorine and bromine based disinfectants, and iodonium based disinfectants. A phenolic disinfectant, a quaternary ammonium disinfectant, and the like.

The food grade disinfecting compositions of the present invention can provide a higher level of bactericidal activity equal to, and in some cases greater than, non-food grade compositions. In one embodiment, the food grade composition can provide a bactericidal level above log 4. In another embodiment, the food grade composition can provide a bactericidal level above log 5. In another embodiment, the food grade composition can provide a bactericidal level above log 6. In yet another embodiment, the food grade composition can provide a bactericidal level above log 7. In yet another embodiment, the food grade composition can provide a bactericidal level above log 8. It is important to note that the level of sterilisation will vary with the composition of the composition as well as the target organism and the substrate being sterilized. In most cases, the level of sterilisation can be achieved within 15 seconds of using the disinfecting composition. Prolonged exposure of the target organism to the disinfecting composition generally results in increased levels of bactericidal activity, however, generally at least a bactericidal level above log 4 can be achieved within 15 seconds of exposure.

The aqueous carrier can optionally include other ingredients such as an organic cosolvent. In particular, specific alcohols may be present. For example, alcohols may be used include aliphatic alcohols having from other 1 to 24 carbons (C ₁ -C _{2 4} alcohols) carbon-containing alcohol. It should be noted that "C ₁ -C _{2 4} alcohol" does not necessarily refer only to a linear saturated aliphatic alcohol. Other carbon-containing alcohols may be used in this definition, including branched aliphatic alcohols, aliphatic rings. Alcohols, aromatic alcohols, unsaturated alcohols and substituted aliphatic alcohols, aliphatic cyclic alcohols, aromatic alcohols, and unsaturated alcohols. In one example, the aliphatic alcohols may be a C ₁ -C ₅ alcohols including methanol, ethanol, propanol, isopropanol, butanol, and pentanol, and readily available because of its low boiling point. That is, polyols have been found to be particularly effective in enhancing the disinfecting and sterilizing ability of the compositions of the present invention and to provide some degree of stability. Without being bound by theory, it is believed that the increased number of hydroxyl groups in the polyol stabilizes the solution due to the action of the aqueous medium and the peracid, increasing the ability to disinfect and sterilize the solution. The increase in hydroxyl groups can also increase the number of hydroxyl radicals or hydroxyl groups in the disinfecting/sterilizing solution to further enhance the ability or killing ability of the solution/dispersion. Examples of polyols useful in the present invention include, but are not limited to, ethylene glycol (ethane-1,2-diol), glycerol (or glycerol, propane-1,2,3-triol), and propane-1. , 2-diol. Other non-aliphatic alcohols which may also be used include, but are not limited to, phenols and substituted phenols, camphorol, castor oil, arachidyl alcohol, octanol, decyl alcohol, behenyl alcohol, dodecanol (1-12) Alcohol), tetradecanol (1-tetradecanol), hexadecyl (or palmitol) alcohol, stearyl alcohol (1-octadecyl alcohol), isostearyl alcohol, oleyl alcohol (cis-9-octadecene-1 -alcohol), palmitoleol, linoleyl alcohol (9Z, 12Z-octadecen-1-ol), oleyl alcohol (9E-octadecen-1-ol), elaidolinoleyl alcohol (9E, 12E-18 En-1-ol), linolenic alcohol (9Z, 12Z, 15Z-octadecaen-1-ol), elaidolinolenyl alcohol (9E, 12E, 15E-octadecan-1-ol), combinations thereof, etc. .

In some specific examples, for practical considerations, it is often preferred to use methanol, ethanol, and denatured alcohols (a mixture of ethanol and less methanol, and optionally a small amount of benzene, ketone) for ease of availability and cost. , acetate, etc.). If you want to provide a food grade composition, you can choose the alcohol that meets this need. The concentration of the alcohol may vary widely, such as from 0 to 95% by weight, but when present, may range from 0.001% to 95% by weight, and more preferably from 1% to 50% by weight. Moreover, it is also possible to use from about 5% by weight to 50% by weight, and still more preferably, it can be used in a range from about 5% by weight to about 15% by weight. Since these ranges are exemplary only, those skilled in the art can vary these ranges for a particular application, considering whether the selected alcohol is a polyol, whether the alcohol is a food grade, a mixture of alcohols, and the like.

With regard to transition metals, in accordance with specific embodiments of the invention, the metal can be in ionic form (e.g., metal salt) and/or colloidal form. In a particular embodiment, the transition metal can be in sub-micron form (i.e., a metal colloidal particle dispersion of less than 1 μm). However, large colloidal transition metal particles can also be used in certain applications. Transition metals which are generally desired to be used include transition metals of Groups VI to XI, and more preferably transition metals of Groups X to XI. Alloys comprising at least one Group VI to Group XI metal may also be used. It has been confirmed that any of these metals are generally oxidized to the corresponding cations in the presence of a peracid. However, generally in the form of colloidal metals, the surface is generally more susceptible to this oxidation. Moreover, when the colloidal metal is dispersed in the colloidal solution, the amount of metal in the form of ions or salts often occurs in the suspension. For example, the colloidal silver may comprise a specific percentage of silver salt or ionic silver in the solution, such as from 10% to 90% by weight, based on the total metal content, of the metal content may be ions. That is, certain preferred metals for use in accordance with embodiments of the present invention are ruthenium, rhodium, iridium, osmium, palladium, platinum, copper, gold, silver, alloys thereof, and mixtures thereof. Depending on the application, the level of sterility desired or desired, the type of target pathogen, the substrate being cleaned, etc., the best is usually silver. Any of these specific examples may be beneficial to the use of the alloy. For example, a combination of specific metals in an alloy can provide an acceptable level of bactericidal activity for a particular pathogen and also provide benefits associated with secondary considerations such as solution stability, the substrate to be cleaned, and the like. Examples of preferred transition metal alloys for use in the present invention include, but are not limited to, copper-silver alloys, silver-manganese alloys, iron-copper alloys, chromium-silver alloys, gold-silver alloys, and magnesium-silver alloys.

The metal content concentration which may be present in the solution including the ion and/or colloid content is from 0.001 ppm to 50,000 ppm by weight based on the total liquid carrier. However, in another embodiment, the concentration of the metal can range from 10 ppm to 1500 ppm by weight. Exemplary colloidal silvers that may be used include the trademarks CS Plus and CS Ultra sold by Solution IE. Other colloidal silver products that can be used as a silver source include ASAP, Sovereign Silver, Silver Max, and the like. Preferred silver salts include, but are not limited to, silver nitrate, barium acetate, silver citrate, silver oxide, and silver carbonate if used in ionic form. In a specific example, the colloidal particles used in the present invention may have a particle size ranging from 0.001 μm to 1.0 μm. In another embodiment, the size of the colloidal transition metal particles may range from 0.030 μm to 0.5 μm. In still other specific examples, the average particle size is from 0.35 μm to 0.45 μm. While any active colloidal silver solution can be used in the formulations of the present invention, in one embodiment, it is desirable to use RO water as the suspension medium for the colloidal silver mixed with the other ingredients. In a more detailed view, the RO water can also be distilled to obtain 18-20 MΩ water, although this is not necessary.

The peracid (or peroxyacid) can be any aliphatic or aromatic peracid which is intended to be compatible with the specific examples of the invention. Any peroxyacid can be used, and peroxyacids containing from 1 to 7 carbons are most practical. These peroxyacids may include, but are not limited to, peroxyformic acid, peroxyacetic acid, peroxyoxalic acid, peroxypropionic acid, perlactic acid, peroxybutyric acid, peroxyvaleric acid, peroxyhexanoic acid, peroxydipic acid, Peroxy citric acid, and / or peroxybenzoic acid, and mixtures thereof. The peroxyacids useful in the present invention can be prepared by any of the methods known in the art. When the peroxyacid is prepared as an acid and hydrogen peroxide, the resulting mixture contains both the peroxyacid and the corresponding acid prepared therefrom. For example, in a specific example using a peroxyacid, the presence of a related acid (acetic acid) provides stability to the mixture, when the reaction is between acid, hydrogen peroxide, and peroxyacid and water, as follows:

The peroxyacid portion of the formulation may range from about 0.001% by weight to about 50% by weight, but more desirably ranges from about 0.001% by weight to about 25% by weight, and most preferably from about 1% by weight to A range of 10% by weight.

Hydrogen peroxide is considered to use more desirable peroxides in accordance with specific embodiments of the invention, and other peroxides such as metal peroxides and peroxyhydrates may also be used. Metal peroxides that may be used include, but are not limited to, sodium peroxide, magnesium peroxide, calcium peroxide, barium peroxide, and/or barium peroxide. Other salts (such as sodium percarbonate) have hydrogen peroxide which is much like the water of hydration, and these can also be regarded as a source of hydrogen peroxide, thus producing hydrogen peroxide in situ. The concentration of the peroxide portion of the preparation may range from about 0.001% to 25% by weight, but ranges from 0.001% to 10% by weight, and further from 1% to 3% by weight. Enough to use.

The disinfecting and sterilizing compositions of the present invention can be prepared for use in a variety of methods, and other ingredients can be incorporated to form a variety of sterile and sterilized products. Examples of disinfecting products include, but are not limited to, hand lotions, mouthwashes, surgical scrubs, body sprays, hand sanitizing gels and foams, sterile wipes, and similar personal care products. Additional product types include disinfectant foams, creams, mousses, etc., as well as compositions containing organic and inorganic fillers, such as milk. Liquid, syrup, cream, cream, etc. The composition can further be used as an antibacterial cleanser for hard surfaces, such as sinks and cabinet countertops in hospitals, food service areas, and meat processing plants. Disinfecting compositions can also be used as disinfecting aerosols and disinfecting aerosols. The antibacterial composition can be manufactured into a diluted ready-to-use composition or a concentrated solution to be diluted before use. The various products in which the disinfectant is used may also include perfume, depending on the characteristics of the product. For example, in the kitchen cleaning wipes, it is desirable to use pine or lemon flavors because it combines the appeal of many consumers with cleanliness. Moreover, gels or aerosols may also be flavored for similar or other reasons.

In one embodiment, the disinfecting composition can be used to make a sterile mouthwash. In addition to disinfecting or sterilizing the composition, the mouthwash may also contain flavors, sweeteners, colors, anti-plaque agents, fluoride ion components, and other medical ingredients. Some metal ions known in the art are used as anti-plaque agents. In addition, for any transition metal ion anti-plaque agent, additional anti-plaque agents include, but are not limited to, sodium lauryl sulfate, triclosan, stannous ion, amyloglucosidase, glucose oxidase, essential oil, or Its combination. Fluoride ion components include, but are not limited to, sodium fluoride, monofluorophosphate, stannous fluoride, and mixtures thereof.

In another embodiment, the disinfecting composition can be used to make an antibacterial toothpaste. Toothpaste can be a semi-aqueous material that removes deposits from the teeth and is typically used in combination with a toothbrush. The toothpaste of the present invention may include an abrasive, a wetting agent, a solvent, a surfactant (detergent), a thickener, a perfume, a whitening agent, an anti-barrier, a sweetener, a pigment, and a fluoride ion component. Examples of anti-plaque agents that can be used in the toothpaste of the present invention include, but are not limited to, metal ions, lauryl sulfate Sodium, triclosan, stannous ion, amyloglucosidase, glucose oxidase, essential oil, or a combination thereof. Fluoride ion components include, but are not limited to, sodium fluoride, monofluorophosphate, stannous fluoride, and mixtures thereof. The toothpaste of the present invention can be in the form of a sauce or a gel.

In another embodiment, the disinfecting composition can be used to make a gel or lozenge. The glue or lozenge of the present invention may have the characteristics of sterilization and sterilization. The gums may comprise perfumes, colors, gum bases, sweeteners, and softeners. The gum base of the present invention can be natural or synthetic. The glue can be coated or uncoated and can be made into any shape or size. Tablets may include perfumes, colors, syrups, sweeteners, hardeners, and the like.

In another embodiment, the disinfecting composition is adjustable to form an anti-corrosive ointment. The antiseptic ointment may be in the form of a gel or cream and may include additional ingredients such as thickeners, moisturizers, colors, and medical agents. Examples of medical agents include, but are not limited to, analgesics, anesthetics, and anti-itch agents. In one embodiment, the ointment can include aloe vera or other known skin ointments. In another embodiment, the ointment can be used in or incorporated into a decorative or skin infiltrating patch.

In another embodiment, the disinfecting composition can be used to make antibacterial hand washing and bath soap. The soap may include additional ingredients such as perfumes, emollients, and/or foaming agents. In one embodiment, the soap is adjustable to form a foam upon dispensing. The viscosity of the soap can be varied by the use of thickeners and surfactants. The soap can be dispensed by any method known in the art, including conventional pumping and foaming dispensers. Alternatively, a hard hand soap can be prepared with the disinfecting composition of the present invention.

In another embodiment of the invention, the disinfecting composition is used to make Poisonous wipes. The sterilizing wipes of the present invention can be used to clean a variety of hard and other surfaces, including human hands and skin, medical instruments, cabinet tops, floors, walls, windows, and the like. The wipes of the present invention can be made from a variety of fabrics. For the purposes of the present invention, cloth is defined to include cloth and paper, as well as woven and non-woven materials. The woven or nonwoven fabric can be made of a suitable material such as enamel, nylon, or cotton, combinations thereof. Examples of non-woven fabrics are described in U.S. Patent Nos. 3,786,615, 4,395,454 and 4,199,322, the disclosures of each of which are incorporated herein by reference. The cloth or paper may be impregnated with a disinfecting solution by any method known in the art. Wipes can be packaged in any manner known in the art, including individual blister packs or folded or laminated multi-packages.

In another embodiment, the disinfecting composition of the present invention is formulated into a gel or gelatinous disinfecting composition. In addition to the disinfecting composition, the gel disinfectant of the present invention may comprise a thickening or gelling agent, wherein the "thickener" and "gelling agent" are used interchangeably. For the purposes of the present invention, the term "gel" or "colloidal" disinfecting composition means a sterilized liquid material having a viscosity of from about 1,000 centipoise to about 100,000 centipoise, or in another embodiment from 2,000. From centipoise to 50,000 centipoise, although these ranges are not intended to be limiting. For example, a hand gel can be less viscous than a gel used for industrial cleaning or disinfecting purposes. Examples of gelling or thickening agents include, but are not limited to, natural rubber of guar and Guanhua bean derivatives, synthetic polymers, clay, oil, wax, aloe vera gel, acrylate homopolymer, acrylate copolymerization. , carbomer, cellulose, cellulose derivatives, alginate, alginate derivatives, water-insoluble C ₈ -C ₂₀ alcohols, red algae, soot, and mixtures thereof, and the like. The gelling agent can be present in the colloidal disinfecting composition in an amount from about 0.1% to about 50% by weight of the gum composition. In another embodiment, the gelling agent is present in an amount from 0.25% to 10% by weight of the gum composition. The amount of gelling agent can be related to a number of factors, including the type of gelling agent and the desired gel viscosity. Gummy disinfectants can be used in many applications, including disinfection of human skin, such as hand sanitizing gels, and disinfection of hard surfaces. In a particular embodiment, the disinfecting composition can be combined with natural aloe vera to form a sterile aloe vera preparation. The preparation can be used for burns, skin infections, and or other irritating applications. Aloe vera may be used as a thickening agent, depending on the desired viscosity of the disinfecting gel, and may also include other thickening or gelling agents as described above.

In still another embodiment, the disinfecting composition of the present invention can be formulated into a sterile foam or foaming composition. The sterilizing foam or foaming composition includes a disinfecting composition and a foaming agent. Any of the blowing agents known in the art can be used and will depend on the desired application and the characteristics of the resulting sterilizing foam. For disinfecting compositions, the disinfecting foams of the present invention can be used in both humans (e.g., hand cleaning) and industrial applications.

In yet another embodiment, the disinfecting composition of the present invention can be in the form of a disinfecting aerosol or aerosol. Fog is also known as hot smoke and is a procedure for aerosolizing disinfectants. The aerosol particles of the disinfectant are suspended in the air for a period of time to sterilize the air itself and the surface, including, for example, structurally inaccessible portions of the air duct. The aerosolized particles of the disinfectant can have a particle size of from about 5 μm to about 200 μm. In another embodiment, the aerosolized particles can have a particle size of from about 20 μm to about 150 μm. When the aerosolized disinfectant contains a colloidal transition metal, the aerosolized particles are generally of sufficient size to contain at least one colloidal transition metal, although typically each aerosolized particle will contain a plurality of colloidal transition metal particles.

Fog is often the final stage of a complete bio-protection program and therefore plays a major role in disease prevention and control. Conventional fogging agents such as formaldehyde, glutaraldehyde, or glutaraldehyde pose important health and safety issues for people exposed to disinfectants. The disinfectant according to the present invention can be used only in food grade ingredients, and it is of great value for use in disinfecting aerosols. Most fogging machines operate using high volume air to produce small droplets at high pressures. The disinfecting compositions of the present invention are compatible with most standard fogging machines. Examples of suitable foggers include Dyna-Fog's Thermal Foggers and Cold Foggers.

As a solution, the composition can be used as a liquid dispersion bath for an object such as an instrument, or as a spray for less moving objects. Disinfecting solutions can also be used as a partial decoration or mouthwash. In other words, any method of use known to those skilled in the art can be used in accordance with the specific embodiments of the present invention.

Additionally, while the compositions of the present invention are primarily described as generally sterile and/or sterilizing agents, many other possible applications have been identified. For example, without limitation, the compositions of the present invention can be used to kill bacteria, spores, viruses, parasites, fungi, and molds. As stated, this potent composition can be used against all of these types of organisms, and is completely safe for humans and other animals.

Because these compositions can be formulated to be very safe, for example, including only food grade ingredients in specific examples, these compositions can be used in areas that are far more extended than those described above. This product category includes products for both local and internal use of both humans and animals. Because it achieves bactericidal levels, even when only food-grade ingredients are modulated, a wide range of pathogenic bacteria and some viruses can be killed internally. For example, these compositions can be used to kill various viruses such as HIV, SARS, West Nile, avian influenza, and others.

EXAMPLES The following examples illustrate the specific examples of the invention that are currently best known. However, it is to be understood that the following are merely exemplary or illustrative applications of the principles of the invention. Many variations and alternative compositions, methods, and systems can be devised without departing from the spirit and scope of the invention. The scope of the proposed patent is to cover this change and arrangement. Thus, the present invention has been described in terms of the foregoing embodiments, and the following examples further provide details relating to what is presently regarded as the most practical and preferred embodiments of the invention.

Example 1 - Preparation of a Disinfectant An aqueous disinfecting composition was prepared in accordance with an embodiment of the present invention comprising an approximate amount of the following components: 85% by weight distilled water containing 600 ppm by weight colloidal silver; 9% by weight ethanol; and 6% by weight Oxyacetic acid. A small amount, that is, <3 wt% of hydrogen peroxide based on the entire aqueous composition was added to the composition to stabilize the peroxyacetic acid. It is noted that there is less than 600 ppm by weight of colloidal silver based on the total aqueous carrier content.

Example 2 - Preparation of a Disinfectant An aqueous disinfecting composition was prepared in accordance with an embodiment of the present invention comprising an approximate amount of the following ingredients: 85% by weight distilled water containing 600 ppm by weight colloidal silver; 9% by weight isopropanol; and 6 weight % peroxypropionic acid. A small amount of sodium peroxide was added to the composition to stabilize the peroxypropionic acid. It is to be noted that there is less than 600 ppm by weight of ionic silver based on the total aqueous carrier content.

Example 3 - Preparation of Disinfectant An aqueous disinfecting composition was prepared in accordance with an embodiment of the present invention comprising an approximate amount of the following ingredients: 75 wt% RO water (reverse osmosis water) containing 1500 ppm by weight colloidal silver; 15 wt% ethanol And 10% by weight of peroxyacetic acid. A small amount of hydrogen peroxide was added to the composition and acetic acid was added to the solution to stabilize the peracetic acid. It is to be noted that there is less than 1500 ppm by weight of colloidal silver based on the total aqueous carrier content.

Example 4 - Preparation of a Disinfectant An aqueous disinfecting composition was prepared in accordance with an embodiment of the present invention comprising an approximate amount of 75 weight percent distilled water containing 10,000 ppm by weight colloidal silver; 20% by weight denatured alcohol; and 5% by weight Peroxyformic acid. A small amount of hydrogen peroxide and formic acid were also added to the entire composition to stabilize the peroxyformic acid. It is to be noted that there is less than 10,000 ppm by weight of colloidal silver based on the total aqueous carrier content.

Example 5 - Preparation of a Disinfectant An aqueous disinfecting composition was prepared in accordance with an embodiment of the present invention comprising an approximate amount of 85% by weight distilled water containing 80 ppm by weight of colloidal silver; 9% by weight of ethanol; and 6% by weight. Oxyacetic acid. A small amount, that is, <3 wt% of hydrogen peroxide based on the entire aqueous composition was added to the composition to stabilize the peroxyacetic acid. It is to be noted that there is less than 80 ppm by weight of colloidal silver based on the total aqueous carrier content.

Example 6 - Study of S. aureus killing time using the disinfectant of Example 1 The antimicrobial activity of the colloidal silver disinfectant of Example 1 was investigated and challenged with organic load on the tested bio-S. aureus. It performed a standard suspension test on a disinfectant containing 5% v/v horse serum. Evaluated with a 15 second contact time.

Specifically, the suspension preparations tested were incubated in Todd Hewitt broth at 37 ° C for 5 hours in 5 ml of S. aureus ATCC 6538 medium. 5 ml of the culture was pelleted by centrifugation, washed with 5 ml of sterilized 18 MΩ water, centrifuged again, and resuspended in a final volume of 5 ml of sterilized water.

A neutralizing agent was prepared which contained 12.7% by weight of Tween 80 (surfactant), 6.0% by weight of Tamol, 1.7% by weight of lecithin, 1% by weight of digested protein, and 0.1% by weight of cystine. A ml tube consisting of 10 pd of catalytic enzyme solution (Sigma, C100, 42,300 units/mg).

The procedure for "kill time" was as follows: A 9.9 ml sample of Example 1 disinfectant (containing 5% v/v horse serum) was placed in a sterile 20 mm x 150 mm tube and the tube was equilibrated in a 20 °C water bath. The tube of the disinfectant was inoculated with 100 μl of test biological suspension at time zero. After 15 seconds, remove 1 ml of the bio/disinfectant suspension into 9 ml of neutralizer. After 2 minutes, the neutralized suspension was serially diluted in physiological saline (PSS) (1:1 × 10, 1:1 × 10 ² , 1:1 × 10 ^{3 ,} etc.). The number of living organisms in the selected dilution tube was analyzed by membrane filtration. A 1 ml sample was placed on the plate in two replicates, and the film was washed with about 100 ml of sterilized PSS and transferred to a Colombian amaranth dish. This plate was incubated at 37 ° C for 20 hours. The number of colonies on each filter was counted and the log reduction and percent kill values were calculated.

A 1:10 dilution of the test suspension in the PSS was selected for membrane filtration analysis, and the titer (or measurement of the amount, or concentration of the substance in the solution) of the test suspension was calculated as a control group. The neutralization control group was inoculated with a mixture of 9 ml of neutralizing agent and 1 ml of disinfectant 100 μl with a titer of 1:10 ⁵ dilution. This produced about 1,500 CFU/ml in the tube, allowed to stand for 20 minutes before dilution, and analyzed for tube filtration using a duplicate 1 ml sample. The sterilization control group was filtered using 100 ml (PSS) or 1 ml (other liquids) for each sample of the solution tested. The plates were incubated as described above.

The results are as follows: TNC ^* - the number is too large to count

The sterilization control group indicated zero growth of neutralizing agent, water, PSS, Colombian agar, disinfectant, and horse serum. Results show live titre in the original suspension concentration aureus per ml ¹ × 10 1 ⁰ creatures. A 9.9 ml disinfectant was inoculated with 100 μl of this suspension to produce a starting concentration of 1 x 10 ⁸ organisms per ml. From the results of these procedures, the log reduction (LR) and percent kill (PK) values can be calculated and calculated using the equation: 1) LR = -Log(S/S ₀ ), where S = live concentration after 45 minutes, And S ₀ = the starting concentration of the living organism at time zero; and 2) PK = (1 - (S / S ₀ )) × 100. These values are shown below.

The neutralization control group data indicates that the test solution has been sufficiently neutralized. The observed count was slightly larger than expected, indicating that the killing of none of the residuals occurred due to the unneutralized disinfectant. Generally, the disinfecting solution tested here has a high antimicrobial activity against S. aureus. It is important to note that this level of activity can be achieved even if the disinfectant is mixed with an organic load containing 5% v/v horse serum. Organic loads (such as 5% v/v horse serum) often adversely affect the antimicrobial action of the disinfectant. In any event, the solution of Example 1 had a logarithmic reduction of more than 7 in living organisms within 15 seconds even in the presence of 5% v/v horse serum.

Example 7 - Using Lysol The study of the killing time of Staphylococcus aureus by spray for determining Lysol The antimicrobial activity of the spray disinfectant on the test bio-S. aureus was investigated. It is carried out in a standard suspension test. Evaluated with a 15 second contact time.

Specifically, the test organisms in the test suspension form were prepared by culturing 5 ml of S. aureus ATCC 6538 medium for 30 hours at 70 ° C in Toddy's gravy. 5 ml of the culture was pelleted by centrifugation, washed with 5 ml of sterilized 18 MΩ water, centrifuged again, and resuspended in a final volume of 5 ml of sterilized water.

A neutralizing agent was prepared which contained 12.7% by weight of Tween 80 (surfactant), 6.0% by weight of Tamol, 1.7% by weight of lecithin, 1% by weight of digested protein, and 0.1% by weight of cystine. Ml tube consists of.

The procedure for "killing time" is as follows: disinfectant (Lysol) A 9.9 ml sample of Brand II disinfectant, Spring Waterfall Scent, Lot #B4194-NJ2; 1413-A3) was placed in a sterile 20 mm x 150 mm tube. The tubes were equilibrated in a 20 ° C water bath. The tube of the disinfectant was inoculated with 100 μl of test biological suspension at time zero. After 15 seconds, remove 1 ml of the bio/disinfectant suspension into 9 ml of neutralizer. After 2 minutes, the neutralized suspension was serially diluted in physiological saline (PSS) (1:1 × 10, 1:1 × 10 ² , 1:1 × 10 ^{3 ,} etc.). The number of living organisms in the selected dilution tube was analyzed by membrane filtration. The 1 ml sample was placed on the plate in two replicates. The film was cleaned with approximately 100 ml of sterilized PSS and transferred to a Colombian dinner plate. This plate was incubated at 37 ° C for 20 hours. The number of colonies on each filter was counted and the log reduction and percent kill values were calculated.

A 1:10 dilution of the test suspension in the PSS was selected for membrane filtration analysis and the titer of the test suspension was calculated as the control group. The neutralization control group was inoculated with a mixture of 9 ml of neutralizing agent and 1 ml of disinfectant 100 μl with a titer of 1:10 ⁵ dilution. This produced about 1,500 CFU/ml in the tube, allowed to stand for 20 minutes before dilution, and analyzed for tube filtration using a duplicate 1 ml sample. The sterilization control group was filtered using 100 ml (PSS) or 1 ml (other liquids) for each sample of the solution tested. The plates were incubated as described above.

The results are as follows: TNC ^* - the number is too large to count

The sterilization control group indicated zero growth of neutralizing agent, water, PSS, Colombian vegetables, and disinfectant. The titer results showed that the live Staphylococcus concentration in the original suspension was 1.47 x ¹⁰⁹ organisms per ml. 9.9 ml of disinfectant was inoculated with 100 μl of this suspension, and the initial concentration in the assay tube was 1.47 × 10 ⁷ organisms per ml. From the results of these procedures, the log reduction (LR) and percent kill (PK) values can be calculated and calculated using the equation: 1) LR = -Log(S/S ₀ ), where S = live concentration after 45 minutes, And S ₀ = the starting concentration of the living organism at time zero; and 2) PK = (1 - (S / S ₀ )) × 100. These values are shown in Table 4 below.

The neutralization control group data indicates that the test solution has been sufficiently neutralized. The observed count was slightly larger than expected, indicating that the killing of none of the residuals occurred due to the unneutralized disinfectant. Usually, Lysol The spray has a high antimicrobial activity against S. aureus. It can reduce the logarithm of living organisms by more than 6 within 15 seconds. It should be noted that this test was carried out under the horse serum organic load without Example 5.

According to the use of Lysol In this comparative example, it is to be noted that this example is an example of a suspension carried out in a closed environment. Because Lysol a large amount of alcohol, Lysol It is better in a closed system than in a generally open air on a hard surface. In contrast, compositions prepared in accordance with specific examples of the present invention contain a significant amount of water (which is vaporized more slowly than the alcohol) and behave similarly to the suspension examples applied to hard surfaces in open air. Therefore, currently Lysol A comparison of the example with Example 6 shows Lysol The activity will be lower in actual use. For example, making Lysol Product Reckitt Benckiser promotes Lysol in his own literature Bacteria can in 30 seconds, kill 99.9% (3 Log _{1 0} reduction) (including Staphylococcus aureus (of MRSA)), and this example shows the suspension within 15 seconds, the level of sterilization is Staphylococcus aureus 99.9999% (6 Log _{1 0} reduction).

Example 8 - Study of spore activity kill time using the disinfectant of Example 1 For the determination of the antimicrobial activity of the silver-containing disinfectant of Example 1, the study was carried out on the bacterial endospores of Bacillus subtilis. It performs a standard kill time suspension test using an endosperm suspension of Bacillus licheniformis. In general, spores are more difficult to kill than normal bacteria.

Test organisms in the form of test suspensions containing endospores of Bacillus licheniformis (ATCC #19659) were prepared. Specifically, endospores are prepared by culturing on a nutrient-like vegetable where additional spore forming reinforcement is added. The plates were harvested with sterile water and the endospores were purified by repeated centrifugation and resuspension in water. It was finally washed in 70% by weight of ethanol for 30 minutes to ensure the death of all growthable bacteria. The spores were resuspended in 0.1% by weight of Tween 80 to prevent agglomeration and stored at 4 ° C until use.

A neutralizing solution was also prepared which consisted of a 9 ml tube containing 12.7% by weight of Tween 80, 6.0% by weight of Tamol, 1.7% by weight of lecithin, 1% by weight of digested protein, and 0.1% by weight of cystine. , and 10 μl of the catalytic enzyme solution (Sigma, C100, 42,300 units/mg) was added before use.

The procedure for "kill time" is as follows: 9.9 ml of the disinfectant sample is placed in a sterile glass culture tube. The tubes were equilibrated in a 20 ° C water bath. The tube of disinfectant was inoculated with 100 μl of spore suspension at time zero. After 1 hour, 1 ml of spore/disinfectant suspension was removed to 9 ml of neutralizer. The tubes are completely mixed. After 2 minutes, the neutralized suspension was serially diluted in physiological saline (PSS) (1:1 × 10, 1:1 × 10 ² , 1:1 × 10 ^{3 ,} etc.). The number of viable spores in the selected dilution tubes was analyzed by membrane filtration. The 1 ml sample was placed on the plate in two replicates. The film was cleaned with approximately 100 ml of sterilized PSS and transferred to a Colombian dinner plate. This plate was incubated at 37 ° C for 20 hours. The number of colonies on each filter was counted and the log reduction and percent kill values were calculated.

A 1:10 dilution of the test suspension in the PSS was selected for membrane filtration analysis, and the titer of the test suspension was calculated as the control group. The neutralization control group was inoculated with a mixture of 9 ml of neutralizing agent and 1 ml of disinfectant 100 μl with a titer of 1:10 ⁵ dilution. This produced about 200 CFU/ml in the tube, allowed to stand for 20 minutes before dilution, and analyzed for tube filtration using a duplicate 1 ml sample.

The results are as follows: TNC ^* - the number is too large to count

The sterilization control group indicated zero growth of water, PSS, Columbian vegetables, and disinfectant. The results of the titer showed that the spore concentration of Bacillus licheniformis in the original suspension was 1.24 × 10 ⁹ spores per ml. 9.9 ml of disinfectant was inoculated with 100 μl of this suspension, and the initial concentration in the assay tube was 1.24 × 10 ⁷ spores per ml. From the results of these procedures, the log reduction (LR) and percent kill (PK) values can be calculated and calculated using the equation: 1) LR = -Log(S/S ₀ ), where the live bioconcentration after S = 1 hour, And S ₀ = the starting concentration of the living organism at time zero; and 2) PK = (1 - (S / S ₀ )) × 100. These values are shown in Table 6 below.

The neutralization control group data shows that the neutralizing agent can sufficiently neutralize the disinfectant. The observed count is larger than expected. The solution of Example 1 had a relatively high sporicidal activity and produced a log reduction of greater than 6 within 15 minutes. Bacillus licheniformis is a species commonly used in sporicidal tests and belongs to the same genus as the organism that causes anthrax. In other words, because of its similarity in genus, Bacillus licheniformis spores have been used as non-pathogenic agents for Bacillus anthracis spores.

Example 9 - Study of the killing time of the R. lovastii using the disinfectant of Example 2 The antimicrobial activity of the silver-containing disinfectant of Example 2 was investigated on the T. elegans of the pathogenic agent of rabbit fever. It performs a standard kill time suspension test using a completely lethal suspension of T. faecalis. Because this organism is a CDC selection agent, all tests were performed in a biosafety level 3 (BSL-3) laboratory for personnel trained in BSL-3 practice and procedures.

A test organism in the form of a test suspension containing T. terrasii (single plant #: 02-1103a) was prepared. The suspension was prepared as follows: Four tryptic soy protein amaranth dishes with 0.1% cystine and 5% sheep blood (TSACB) were inoculated whole from isolated colonies in a manufacturing process that had been subjected to Gram staining to ensure purity. On the plate. This plate was incubated at 37 ° C for 48 hours. The growth on each of the four disks was scraped into the suspension using 3 ml of physiological saline (PSS) and a curved loop. Pipette the suspension into a 50 ml conical centrifuge tube. All four trays of suspension were collected into a single tube. The tube was centrifuged at 3,845 x g for 7 minutes in an aerosol-sealed rotor. The supernatant was removed and the pellet was resuspended in 4 ml of PSS. The suspension was kept at 4 ° C until use.

A neutralizing agent was also prepared which contained 12.7% by weight of Tween 80, 6.0% by weight of Tamol, 1.7% by weight of lecithin, 1% by weight of digested protein, 1.0% by weight of cystine, and 500 mM Tris (pH). 7.7) A 9 ml tube consisting of 100 μl of catalytic enzyme solution (Sigma, C100, 42,300 units/mg) before use.

The procedure for "kill time" is as follows: A 9.9 ml sample of the Example 2 disinfectant is placed in a sterile glass culture tube. The tubes were equilibrated in a 20 ° C water bath. The tube of the disinfectant was inoculated with 1.0 ml of the test biological suspension at time zero. After 15 seconds and 30 seconds, remove 1 ml of the test organism/disinfectant suspension into 9 ml of neutralizer. The tubes are completely mixed. After 2 minutes, the neutralized suspension was serially diluted in physiological saline (PSS) (1:1 × 10, 1:1 × 10 ² , 1:1 × 10 ^{3 ,} etc.). The number of viable spores in the selected dilution tube was analyzed by membrane filtration. The 1 ml sample was placed on the plate in two replicates. The film was cleaned with approximately 100 ml of sterilized PSS and transferred to the TSACB Asian dish. This plate was incubated at 37 ° C for 72 hours. The number of colonies on each filter was counted and the log reduction and percent kill values were calculated.

A 1:10 dilution of the test suspension in the PSS was selected for membrane filtration analysis, and the titer of the test suspension was calculated as the control group. The neutralization control group was inoculated with a mixture of 9 ml of neutralizing agent and 1 ml of disinfectant 100 μl with a titration of 1:1 × 10 ⁵ dilution. This produced approximately 7,110 colony forming units (CFU) per ml in the tube, allowed to stand for 20 minutes before dilution, and analyzed by membrane filtration using repeated 1 ml samples.

The results are as follows: TNC ^* - the number is too large to count

Results show live titre of the suspension in the original soil solution Lavin coli per ml at a concentration of 7.25 × 10 ^{1 0} CFU. A 9.0 ml disinfectant was inoculated with 1.0 ml of this suspension, and the initial concentration in the assay tube was 7.25 x ¹⁰⁹ CFU per ml. From the results of these procedures, the log reduction (LR) and percent kill (PK) values can be calculated and calculated using the equation: 1) LR = -Log(S/S ₀ ), where S = live after the specified contact time Biological concentration, and S ₀ = live biological starting concentration at time zero; and 2) PK = (1 - (S / S ₀ )) × 100. These values are shown in Table 8 below.

The neutralization control group data showed a similar count as expected, with an average of 573 CFU and an expected 711 CFU. This indicates that the neutralizing solution used successfully neutralized the disinfecting solution in these tests. This solution exhibited a fairly rapid rate of killing of the T. faecalis. It can produce a log reduction of more than 8 within 15 seconds, which will completely kill the system used.

Example 10 - Study of Killing Time of Yersinia pestis Using the Disinfectant of Example 2 The antimicrobial activity of the silver-containing disinfectant of Example 2 was investigated on the plaque plaque pathogen Y. pestis. It performs a standard kill time suspension test using a completely lethal suspension of Y. pestis. Because this organism is a CDC selection agent, all tests were performed in a biosafety level 3 (BSL-3) laboratory for personnel trained in BSL-3 practice and procedures.

Test organisms containing the test suspension form of Y. pestis (single plant #: 83-1880a) were prepared as follows: Four Colombian vermicelli dishes were inoculated from isolated colonies in whole pieces and subjected to Gram stain to ensure purity. Made on the plate. The plate was incubated at 28 ° C for 48 hours under 5% CO ₂ . The growth on each of the four disks was scraped into the suspension using 3 ml of physiological saline (PSS) and a curved loop. Pipette the suspension into a 50 ml conical centrifuge tube. Each plate was washed with an additional 2 ml of PSS and also added to a 50 ml tube. All four trays of suspension were collected into a single tube. The tube was centrifuged at 3,845 x g for 7 minutes in an aerosol-sealed rotor. The supernatant was removed and the pellet was resuspended in 4 ml of PSS. The suspension was kept at 4 ° C until use.

A neutralizing agent was also prepared which contained 12.7% by weight of Tween 80, 6.0% by weight of Tamol, 1.7% by weight of lecithin, 1% by weight of digested protein, 1.0% by weight of cystine, and 500 mM Tris (pH). 7.7) A 9 ml tube consisting of 100 μl of catalytic enzyme solution (Sigma, C100, 42,300 units/mg) before use.

The procedure for "kill time" is as follows: A 9.9 ml sample of the Example 2 disinfectant is placed in a sterile glass culture tube. The tubes were equilibrated in a 20 ° C water bath. The tube of the disinfectant was inoculated with 1.0 ml of the test biological suspension at time zero. After 15 seconds and 30 seconds, remove 1 ml of the test organism/disinfectant suspension into 9 ml of neutralizer. The tubes are completely mixed. After 2 minutes, the neutralized suspension was serially diluted in physiological saline (PSS) (1:1 × 10, 1:1 × 10 ² , 1:1 × 10 ^{3 ,} etc.). The number of viable spores in the selected dilution tube was analyzed by membrane filtration. The 1 ml sample was placed on the plate in two replicates. The film was cleaned with approximately 100 ml of sterilized PSS and transferred to a Colombian dinner plate. The plate was incubated at 37 ° C under 5% CO _{2 for} 48 hours. The number of colonies on each filter was counted and the log reduction and percent kill values were calculated.

A 1:10 dilution of the test suspension in the PSS was selected for membrane filtration analysis, and the titer of the test suspension was calculated as the control group. The neutralization control group was inoculated with a mixture of 9 ml of neutralizing agent and 1 ml of disinfectant 100 μl with a titration of 1:1 × 10 ⁵ dilution. This produced approximately 3,380 colony forming units (CFU) per ml in the tube, allowed to stand for 20 minutes before dilution, and analyzed by membrane filtration using repeated 1 ml samples.

The results are as follows: TNC ^* - the number is too large to count

Results show live titre in the original suspension solution Yersinia bacillus per ml concentration of 3.45 × 10 ^{1 0} CFU. 9.0 ml of disinfectant was inoculated with 1.0 ml of this suspension, and the initial concentration in the assay tube was 3.45 x ¹⁰⁹ CFU per ml. From the results of these procedures, the log reduction (LR) and percent kill (PK) values can be calculated and calculated using the equation: 1) LR = -LOg(S/S ₀ ), where S = live after the specified contact time Biological concentration, and S ₀ = live biological starting concentration at time zero; and 2) PK = (1 - (S / S ₀ )) × 100. These values are shown in Table 10 below.

The neutralization control group data showed a similar count as expected, yielding an average of 57 CFU and expected to be approximately 338 CFU. In tests with other organisms, because this same preparation successfully neutralized the disinfectant of Example 2, the study began to find that it is possible that the mixture itself is specifically toxic to any Y. pestis. The disinfectant of Example 2 exhibited a relatively fast killing rate of Y. pestis. It can produce a log reduction of more than 8 within 15 seconds, which will completely kill the system used.

Example 11 - Study on the killing time of Brucella abortus using the disinfectant of Example 2 On the Brucella abortus of the pathogenic agent of fluctuating heat or brucellosis, the anti-oxidation inhibitor of Example 2 was determined. Microbial activity was studied. It performs a standard kill time suspension test using a completely lethal suspension of Brucella abortus. Because this organism is a CDC selection agent, all tests were performed in a biosafety level 3 (BSL-3) laboratory for personnel trained in BSL-3 practice and procedures.

A test organism in the form of a test suspension containing Brucella abortus (698 strain 544) was prepared. The suspension was prepared as follows: Four Brucella sinensis (BBA) dishes were inoculated whole from isolated colonies onto a manufacturing plate that had been subjected to Gram stain to ensure purity. The plate was incubated at 37 ° C for 48 hours under 5% CO ₂ . The growth on each of the four disks was scraped into the suspension using 3 ml of physiological saline (PSS) and a curved loop. Pipette the suspension into a 50 ml conical centrifuge tube. Each plate was washed with an additional 2 ml of PSS and also added to a 50 ml tube. All four trays of suspension were collected into a single tube. The tube was centrifuged at 3,845 x g for 7 minutes in an aerosol-sealed rotor. The supernatant was removed and the pellet was resuspended in 4 ml of PSS. The suspension was kept at 4 ° C until use.

A neutralizing agent was also prepared which contained 12.7% by weight of Tween 80, 6.0% by weight of Tamol, 1.7% by weight of lecithin, 1% by weight of digested protein, 1.0% by weight of cystine, and 500 mM Tris (pH). 7.7) A 9 ml tube consisting of 100 μl of catalytic enzyme solution (Sigma, C100, 42,300 units/mg) before use.

The procedure for "kill time" is as follows: A 9.9 ml sample of the Example 2 disinfectant is placed in a sterile glass culture tube. The tubes were equilibrated in a 20 ° C water bath. The tube of the disinfectant was inoculated with 1.0 ml of the test biological suspension at time zero. After 15 seconds and 30 seconds, remove 1 ml of the test organism/disinfectant suspension into 9 ml of neutralizer. The tubes are completely mixed. After 2 minutes, the neutralized suspension was serially diluted in physiological saline (PSS) (1:1 × 10, 1:1 × 10 ² , 1:1 × 10 ^{3 ,} etc.). The number of viable spores in the selected dilution tube was analyzed by membrane filtration. The 1 ml sample was placed on the plate in two replicates. The film was cleaned with approximately 100 ml of sterilized PSS and transferred to a Colombian dinner plate. The plate was incubated at 37 ° C for 48 hours under 5% CO ₂ . The number of colonies on each filter was counted and the log reduction and percent kill values were calculated.

A 1:10 dilution of the test suspension in the PSS was selected for membrane filtration analysis, and the titer of the test suspension was calculated as the control group. The neutralization control group was inoculated with a mixture of 9 ml of neutralizing agent and 100 ml of disinfectant 100 μl with a titration of 1:1 × 10 ⁶ dilution. This produced approximately 290 colony forming units (CFU) per ml in the tube, allowed to stand for 20 minutes before dilution, and analyzed by membrane filtration using repeated 1 ml samples.

The results are as follows: TNC ^* - the number is too large to count

The titer results showed that the live abortive Brucella concentration in the original suspension solution was 2.96 x 10 ^{1 1} CFU per ml. The 9.0 ml of disinfectant with 1.0 ml of this suspension was inoculated, producing an initial concentration of the analysis tube per ml was 2.96 × 10 ^{1 0} CFU. From the results of these procedures, the log reduction (LR) and percent kill (PK) values can be calculated and calculated using the equation: 1) LR = -Log(S/S ₀ ), where S = live after the specified contact time Biological concentration, and S ₀ = live biological starting concentration at time zero; and 2) PK = (1 - (S / S ₀ )) × 100. These values are shown in Table 12 below.

The neutralization control group data showed a similar count as expected, with an average of 1,915 CFU and an expected 290 CFU. This indicates that the neutralizing solution used successfully neutralized the disinfectant of Example 2 of these tests. The disinfectant of Example 2 exhibited a fairly rapid rate of killing of Brucella abortus. It can produce a log reduction greater than 9 within 15 seconds, and the system it will use is almost completely killed.

Example 12 - Study of the time to kill of Bacillus anthracis using the disinfectant of Example 2 The antimicrobial activity of the silver-containing disinfectant of Example 2 was investigated on bacterial spores from Bacillus anthracis. It performs a standard kill time suspension test using a suspension of completely endogenously purified endospores from Bacillus anthracis. Because of the large concentration of lethal spores, all tests were performed in a biosafety level 3 (BSL-3) laboratory for personnel trained in BSL-3 practice and procedures.

Test organisms in the form of a spore test suspension containing Bacillus anthracis (A0256) were prepared by growing 250 ml of the culture medium in a Leighton Doi medium in a 2 L conical flask. The bottle was shaken at 100 RPM for 3 to 5 days at 37 ° C until 90% sporulation was observed by phase contrast microscopy. Spores were harvested and washed three times with sterile HPLC water and stored overnight at 4 °C. Three additional washes were performed, and the suspension was allowed to stand overnight at 4 ° C between each wash. Spores were resuspended in a total of 80 ml of sterile HPLC water until use.

A neutralizing agent was also prepared which contained 12.7% by weight of Tween 80, 6.0% by weight of Tamol, 1.7% by weight of lecithin, 1% by weight of digested protein, 1.0% by weight of cystine, and 500 mM Tris (pH). 7.7) A 9 ml tube consisting of 100 μl of catalytic enzyme solution (Sigma, C100, 42,300 units/mg) before use.

The procedure for "kill time" is as follows: A 4.5 ml sample of the Example 2 disinfectant is placed in a sterile glass culture tube. The tubes were equilibrated in a 20 ° C water bath. The tube of the disinfectant was inoculated with 0.5 ml of spore suspension at time zero. After 15 seconds and 30 seconds, remove 1 ml of the spore/disinfectant suspension into 9 ml of neutralizer. The tubes are completely mixed. After 2 minutes, the neutralized suspension was serially diluted in physiological saline (PSS) (1:1 × 10, 1:1 × 10 ² , 1:1 × 10 ^{3 ,} etc.). The number of viable spores in the selected dilution tube was analyzed by membrane filtration. The 1 ml sample was placed on the plate in two replicates. The film was cleaned with approximately 100 ml of sterilized PSS and transferred to a Colombian dinner plate. This plate was incubated at 37 ° C for 20 hours. The number of colonies on each filter was counted and the log reduction and percent kill values were calculated.

A 1:10 dilution of the test suspension in the PSS was selected for membrane filtration analysis, and the titer of the test suspension was calculated as the control group. The neutralization control group was inoculated with a mixture of 9 ml of neutralizing agent and 1 ml of disinfectant 100 μl with a titration of 1:1 × 10 ⁵ dilution. This produced approximately 360 colony forming units (CFU) per ml in the tube, allowed to stand for 20 minutes before dilution, and analyzed by membrane filtration using repeated 1 ml samples.

The results are as follows: TNC ^* - the number is too large to count

The results of the titer showed that the concentration of live Bacillus anthracis spores in the original suspension solution was 3.70 x ¹⁰⁹ spores per ml. A 4.5 ml disinfectant was inoculated with 1.0 ml of this suspension, and the initial concentration in the assay tube was 3.70 x 10 ⁸ spores per ml. From the results of these procedures, the log reduction (LR) and percent kill (PK) values can be calculated and calculated using the equation: 1) LR = -Log(S/S ₀ ), where S = live after the specified contact time Biological concentration, and S ₀ = live biological starting concentration at time zero; and 2) PK = (1 - (S / S ₀ )) × 100. These values are shown in Table 14 below.

Neutralization control group data shows that the neutralizer can fully neutralize the disinfectant. The observed count was higher than expected (63 vs. 36, respectively). The disinfectant of Example 2 had fairly rapid sporicidal activity against anthrax spores. It can produce a log reduction greater than 7 within 30 seconds, which will use a system that is nearly completely killed. The disinfectant of Example 2 exhibited an extremely fast rate of spore killing of B. anthracis compared to other general chemical disinfectants. To make this inference correct, previous data from spores from this same strain were used, showing that alkaline glutaraldehyde (diluted to its minimum effective concentration of 1.5%) required a log reduction of 6 to take 50 minutes.

Example 13 - Study on sporicidal activity kill time using 2.4% alkaline glutaraldehyde disinfectant On the bacterial spores from the test Bacillus subtilis, the antimicrobial activity of the 2.4% alkaline glutaraldehyde disinfectant was determined. the study. The glutaraldehyde disinfection solution is a disinfectant commonly used in hospitals to kill bacteria and other pathogenic bacteria, otherwise the bacteria are difficult to kill. This study performed a standard kill time suspension test using a suspension of Bacillus licheniformis spores. Evaluate the contact time of 15 minutes.

A test solution containing endospores of Bacillus licheniformis (ATCC #19659) was prepared from the culture grown on the nutrient leaf, in which additional spore forming reinforcement was added. The plates were harvested with sterile water and the endospores were purified by repeated centrifugation and resuspension in water. It was finally washed in 70% by weight of ethanol for 30 minutes to ensure the death of all growthable bacteria. The spores were resuspended in 0.1% by weight of Tween 80 to prevent agglomeration and stored at 4 ° C until use.

The preparation of the neutralizer consisted of 1 ml freshly prepared, filter sterilized 5.28 wt% sodium hydrogen sulfite.

The procedure for "kill time" is as follows: 9.9 ml of the disinfectant sample is placed in a sterile glass culture tube. The tubes were equilibrated in a 20 ° C water bath. 9 ml of 2.4% by weight alkaline glutaraldehyde disinfectant (freshly activated CIDEXPLUS, 3.4%, Lot#: 2002247TP - diluted to 2.4% by weight with sterile water) tube with 100 μl of test organism suspension at time zero Liquid inoculation. After 15 minutes, 1 ml of the spore/disinfectant suspension was removed into 9 ml of neutralizer. The tubes are completely mixed. After 2 minutes, the neutralized suspension was serially diluted in physiological saline (PSS) (1:1 × 10, 1:1 × 10 ² , 1:1 × 10 ^{3 ,} etc.). The number of viable spores in the selected dilution tubes was analyzed by membrane filtration. The 1 ml sample was placed on the plate in two replicates. The film was cleaned with approximately 100 ml of sterilized PSS and transferred to a Colombian dinner plate. This plate was incubated at 37 ° C for 20 hours. The number of colonies on each filter was counted and the log reduction and percent kill values were calculated.

A 1:10 dilution of the test suspension in the PSS was selected for membrane filtration analysis, and the titer of the test suspension was calculated as the control group.

The neutralization control group was inoculated with a mixture of 1 ml of neutralizing agent and 1 ml of disinfectant 100 μl with a titer of 1:10 ⁵ dilution. This produced approximately 450 CFU/ml in the tube, allowed to stand for 20 minutes before dilution, and analyzed for tube filtration using a replicate 1 ml sample.

The results are as follows: TNC ^* - the number is too large to count

The sterilization control group indicated zero growth of pentanediol, sodium bisulfite, water, PSS, and Colombian agar. The results of the titer showed that the spore concentration of Bacillus licheniformis in the original suspension was 9.45 x 10 ⁸ spores per ml. 9.9 ml of disinfectant was inoculated with 100 μl of this suspension, and the initial concentration in the assay tube was 9.45 × 10 ⁶ spores per ml. From the results of these procedures, the log reduction (LR) and percent kill (PK) values can be calculated and calculated using the equation: 1) LR = -Log(S/S ₀ ), where the live bioconcentration after S = 1 hour, And S ₀ = the starting concentration of the living organism at time zero; and 2) PK = (1 - (S / S ₀ )) × 100. These values are shown in Table 16 below.

The neutralization control group data shows that the neutralizing agent can sufficiently neutralize the disinfectant. The observed count is larger than expected. The 2.4 wt% alkaline pentanediol solution tested had a rather slow sporicidal activity with only a log reduction of 0.48 in 15 minutes.

Example 14 - Disinfecting Mouthwash A disinfecting mouthwash was made using the disinfecting composition as described in Example 1. The mouthwash is prepared by mixing a disinfecting composition containing sorbitol (sweetener), sodium fluoride (fluoride ion component) in an amount sufficient to provide 250 ppm of fluoride ions, and peppermint oil (fragrance). This ingredient was mixed with the disinfecting composition of Example 1 diluted 1:10 by weight of water. It should be noted that the total composition was diluted 1:10 by weight of water, and the content of colloidal silver was significantly lowered. If a higher weight percentage of colloidal silver is desired, the silver content can be adjusted to be higher than in Example 1, such that when the mouthwash is diluted, there will be a higher silver content in the solution.

Example 15 - Disinfecting Toothpaste A sterilizing toothpaste was produced using the sterilizing composition of Example 2. The toothpaste is prepared by mixing the disinfecting composition of claim 2 with water, hydrated silicone, sorbitol, glycerin, sodium lauryl sulfate, titanium dioxide, menthol, pentasodium triphosphate, and PEG-6. The ingredients are mixed together in an amount sufficient to produce a toothpaste having sterilizing properties. Again, it is important to note that the total composition is diluted with toothpaste formation and other components, and the ionic silver content is significantly reduced. If a higher weight percentage of silver is desired, the silver content can be adjusted to be higher than in Example 2 such that a higher silver content is present in the toothpaste when the toothpaste is prepared.

Example 16 - Disinfecting Ointment/Gel Use the disinfecting solution of Example 2 to prepare a disinfecting ointment. The disinfectant of Example 2 was mixed with aloe vera gel to form a disinfectant ointment. The gel is then applied to the infection on the skin of the object. The disinfectant ointment disinfects the skin and provides some relief from the irritation of the infection.

Example 17 - Disinfecting soap A disinfecting liquid soap was prepared using the disinfecting solution of Example 1. Example 1 disinfectant with water, sodium lauryl ether sulfate, sodium lauryl sulfate, sodium sulfate, cocamidopropyl betaine, citric acid, sodium chloride, spices, DMDM allantoin Mix with EDTA and sodium sulphate to produce a disinfectant liquid soap. This soap has a viscosity that makes it easy to dispense using conventional pump soap dispensers. Using the disinfectant of Example 1 as a component used in the soap forming method, a hard hand soap can be prepared in the same manner.

Example 18 - Disinfecting Wipes

A disinfecting wipe was prepared using the disinfecting solution of Example 1. The nonwoven cotton cloth was impregnated with the disinfecting solution of Example 1. The wipes are prepared by placing the laminated cotton sheets in a container, saturating the sheets with a disinfecting solution, capping the containers and sealing the container to prevent vaporization of the disinfecting solution. Since the disinfecting solution of Example 1 included colloidal silver, care must be taken to ensure that each piece of nonwoven cotton cloth was not only exposed to liquid but also to solid particles.

Example 19 - Disinfectant Spray

The disinfecting composition of Example 2 was used to form a disinfectant spray. Using a Dyno-Fog® thermal fogger, the disinfecting composition is aerosolized into small droplets in a small room, such as a hospital room, where disinfection is required. Fill the room with a disinfectant spray. The disinfectant spray sterilizes and disinfects the air and hard surfaces in the room. After a period of about 40 minutes, the aerosolized particles substantially clean the air and the chamber has been substantially sterilized.

The invention has been described with reference to a particular preferred embodiment thereof, and it will be appreciated by those skilled in the art that many variations, changes, and/or substitutions can be made without departing from the spirit of the invention. Therefore, the invention is not limited to the scope of the claimed invention.

Claims (69)

An aqueous disinfecting or sterilizing composition comprising: a) an aqueous carrier comprising: i) water; ii) from 0.001% to 50% by weight of peracid; and iii) from 0.001% to 25% by weight An oxide; b) a colloidal transition metal or alloy thereof from 0.001 ppm to 50,000 ppm by weight based on the aqueous carrier content. The composition of claim 1, wherein the composition is substantially free of aldehyde. The composition of claim 1, wherein the composition comprises an aldehyde. The composition of claim 1, wherein the disinfecting composition is substantially free of at least one self-containing chlorine component, a bromine-containing component, an iodophore-containing component, a phenol-containing component, and a quaternary ammonium-containing component. Selected components of the group. The composition of claim 1, wherein the disinfecting composition is substantially free of all self-containing aldehyde components, chlorine-containing components, bromine-containing components, iodine-containing components, phenol-containing components, and quaternary ammonium-containing components. Selected components of the group. The patentable scope of application of the composition according to item 1, further comprising from 0.001 wt% to 95 wt% of C ₁ -C ₂₄ alcohol as part of the aqueous carrier. The composition of claim 6, wherein the C ₁ -C ₂₄ alcohol is selected from the group consisting of methanol, ethanol, propanol, butanol, pentanol, polyol, aromatic alcohol, and mixtures thereof. The composition of claim 6, wherein the C ₁ -C ₂₄ alcohol is a polyol. The patentable scope of application of the composition according to item 6, wherein the portion contains from 1% to 50% by weight of the C ₁ -C ₂₄ alcohol as the aqueous carrier. The composition of claim 1, wherein the colloidal transition metal or alloy thereof is a transition metal of Group VI to Group XI or an alloy thereof. The composition of claim 1, wherein the colloidal transition metal or alloy thereof is a transition metal of Group X to Group XI or an alloy thereof. The composition of claim 1, wherein the colloidal transition metal or alloy thereof is a group consisting of ruthenium, rhodium, iridium, osmium, palladium, platinum, copper, gold, silver, alloys thereof, and mixtures thereof. Elected in the middle. The composition of claim 1, wherein the colloidal transition metal or alloy thereof is colloidal silver. The composition of claim 1, wherein the colloidal transition metal or alloy thereof has an average particle size of from 0.001 μm to 1.0 μm. The composition of claim 1, wherein the colloidal transition metal or alloy thereof is contained in an amount of from 15 ppm to 1500 ppm by weight. The composition of claim 1, wherein the peracid is an aliphatic or aromatic peroxyacid. The composition of claim 1, wherein the peracid is from peroxyformic acid, peracetic acid, peroxyoxalic acid, peroxypropionic acid, perlactic acid, peroxybutyric acid, peroxyvaleric acid, peroxygen Selected from the group consisting of acid, peroxydipic acid, peroxycitric acid, peroxybenzoic acid, and mixtures thereof. The composition of claim 1, wherein the peracid is contained in an amount of from 0.001% by weight to 25% by weight as part of the aqueous carrier. The composition of claim 1, wherein the peroxide is hydrogen peroxide. The composition of claim 1, wherein the peroxide is a metal peroxide. The composition of claim 20, wherein the metal peroxide is selected from the group consisting of sodium peroxide, magnesium peroxide, calcium peroxide, barium peroxide, barium peroxide, and mixtures thereof. The composition of claim 1, wherein the peroxide is peroxyhydrate. The composition of claim 1, wherein the peroxide is produced in situ. The composition of claim 23, wherein the peroxide is hydrogen peroxide produced from sodium percarbonate. The composition of claim 1, wherein the peroxide is contained in an amount of from 0.001% by weight to 10% by weight as part of the aqueous carrier. The composition of claim 1, wherein the composition provides endospores targeted to Staphylococcus aureus, T. faecalis, Yersinia pestis, Brucella abortus, or Bacillus anthracis within 15 seconds The bactericidal level is log 4 or higher. The composition of claim 1, wherein the target is Staphylococcus aureus, and the bactericidal level within 15 seconds of contact with the target is higher than log 7. The composition of claim 1, wherein the target is T. vivax, and the bactericidal level is higher than log 6 within 15 seconds of contact with the target. The composition of claim 28, wherein the bactericidal level after 15 seconds is higher than log 8 when in contact with the target. The composition of claim 1, wherein the target is Yersinia pestis, and the bactericidal level is higher than log 8 within 15 seconds of contact with the target. The composition of claim 1, wherein the target is Brucella abortion, and the bactericidal level is higher than log 9 within 15 seconds of contact with the target. The composition of claim 1, wherein the target is an endosporin of Bacillus anthracis, and the bactericidal level within 30 seconds of contact with the target is higher than log 7. The composition of claim 1, wherein the composition is formulated into a buccal disinfectant or a cleansing agent for therapeutic use in the oral cavity. Such as the composition of claim 33, wherein the oral cavity Disinfectants or cleansers include perfumes. The composition of claim 33, wherein the oral disinfectant or cleaning agent comprises an antiplaque agent. The composition of claim 33, wherein the oral disinfectant or cleaning agent comprises a fluoride ion component. Such as the composition of claim 33, the oral disinfectant or detergent is prepared in a form selected from the group consisting of mouthwash, toothpaste, gum, and lozenge. The composition of claim 1, wherein the composition is formulated into a disinfecting ointment or gel comprising a thickening agent and formulated to be applied therapeutically to the skin or mucosal surface. The composition of claim 38, wherein the thickener is natural rubber, synthetic polymer, clay, oil, wax, aloe vera, aloe vera gel, acrylic acid of guar and Guanhua bean derivatives. Ester homopolymer, acrylate copolymer, carbomer, cellulose, cellulose derivative, alginate, alginate derivative, water-insoluble C ₈ -C ₂₀ alcohol, red algae, haze And a group consisting of a mixture of them. The composition of claim 38, wherein the thickener is contained in an amount of from 0.1% by weight to 50% by weight. The composition of claim 38, wherein the ointment or gel comprises an analgesic. The composition of claim 38, wherein the ointment or gel comprises an anti-itch agent. Such as the composition of claim 1 of the scope of the patent, wherein the composition It is formulated into a sterilizing soap which is prepared to be applied therapeutically to the surface of the skin. The composition of claim 1, wherein the composition is impregnated into a fabric to form a sterile wipe. The composition of claim 44, wherein the cloth is a nonwoven fabric. The composition of claim 44, wherein the cloth comprises nylon, tantalum, cotton, or a combination thereof. The composition of claim 44, wherein the cloth is paper. The composition of claim 1, wherein the composition is aerosolized into a disinfectant. The composition of claim 48, wherein the aerosolized disinfectant has a particle size of from about 5 μm to about 200 μm. A method of disinfecting a surface comprising contacting the surface with a disinfecting composition comprising: a) an aqueous carrier comprising: i) water; ii) from 0.001% to 50% by weight of peracid; and iii From 0.001% to 25% by weight of peroxide; b) from 0.001 ppm to 50,000 ppm by weight of the colloidal transition metal or alloy thereof based on the aqueous carrier content. The method of application of the scope of patent 50, wherein the sterilizing composition further comprises from 0.001 wt% to 95 wt% of a C ₁ -C ₂₄ alcohol part of the aqueous carrier. The method of claim 50, wherein the C ₁ -C ₂₄ alcohol is selected from the group consisting of methanol, ethanol, propanol, butanol, pentanol, polyol, aromatic alcohol, and mixtures thereof. The method of claim 50, wherein the colloidal transition metal or alloy thereof is a transition metal of Group VI to Group XI or an alloy thereof. The method of claim 50, wherein the colloidal transition metal or alloy thereof is in the group consisting of ruthenium, rhodium, iridium, osmium, palladium, platinum, copper, gold, silver, alloys thereof, and mixtures thereof. Elected. The method of claim 50, wherein the colloidal transition metal or alloy thereof is colloidal silver. The method of claim 50, wherein the colloidal transition metal or alloy thereof is from 15 ppm to 1500 ppm by weight. The method of claim 50, wherein the peracid is from peroxyformic acid, peracetic acid, peroxyoxalic acid, peroxypropionic acid, perlactic acid, peroxybutyric acid, peroxyvaleric acid, peroxyhexanoic acid. Selected from the group consisting of peroxydipic acid, peroxycitric acid, peroxybenzoic acid, and mixtures thereof. The method of claim 50, wherein the peracid is contained in an amount of from 0.001% by weight to 25% by weight as part of the aqueous carrier. The method of claim 50, wherein the peroxide is hydrogen peroxide. The method of claim 50, wherein the peroxide is a metal peroxide. The method of claim 60, wherein the metal peroxide is selected from the group consisting of sodium peroxide, magnesium peroxide, calcium peroxide, barium peroxide, barium peroxide, and mixtures thereof. The method of claim 50, wherein the peroxide is peroxyhydrate. The method of claim 50, wherein the peroxide is produced in situ. The method of claim 63, wherein the peroxide is hydrogen peroxide produced from sodium percarbonate. The method of claim 50, wherein the peroxide is contained in an amount of from 0.001% by weight to 10% by weight as part of the aqueous carrier. The method of claim 50, wherein the disinfecting composition is substantially free of aldehyde. The method of claim 50, wherein the disinfecting composition is substantially free of all self-containing aldehyde components, chlorine-containing components, bromine-containing components, iodine-containing components, phenol-containing components, and quaternary ammonium-containing components. The ingredients selected in the group. The method of claim 50, wherein the surface is a hard surface. The method of claim 50, wherein the surface is a skin or mucosal surface.