JP2014047180A - Edible sterilization agent and edible sterilization agent set - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an edible sterilization agent which is excellent in portability and have a high sterilization effect in spite of being alcohol free and edible sterilization agent set including the edible sterilization agent.SOLUTION: An edible sterilization agent in powdery or tablet form comprising an organic acid, powdery lactic acid and quaternary ammonium chloride is provided. In the sterilization agent, the organic acid comprises one or more selected from the group consisting of citric acid, sodium citrate, sodium iron citrate, tartaric acid, lactic acid, malic acid and adipic acid and the powdery lactic acid comprises one or more selected from the group consisting of lactic acid, calcium lactate and ion lactate and a fine powdery silicon dioxide.

Description

  The present invention relates to a powder or tablet edible sanitizer and an edible sanitizer set.

Conventionally, many disinfectants and bactericides that do not affect the human body have been created. For example, the food disinfectant described in Patent Document 1 contains two or more of edible acids, edible acid salts and ethanol, so that fresh foods such as fresh seafood and fruits and vegetables can be sterilized and statically removed. The purpose is to do fungus.
The sterilizing composition (food disinfectant) described in Patent Document 2 contains two or more sterilizing components selected from the group consisting of organic acids, organic acid salts, electrolytes, and alcohols. By using ingredients designated as food additives, it can be widely used in the field of food industry, and can control or prevent humans from food poisoning and has excellent safety and sterilization method for food field and sterilization The purpose is to provide a composition.

Patent Literature 1 or 2 in which these food sanitizers are disclosed discloses a food sanitizer containing an organic acid and an alcohol.
As disclosed in the specification, these Patent Documents 1 and 2 obtain a food disinfectant having a high disinfecting effect by blending three or more kinds of organic acids, other organic acids and alcohols. This is the essential purpose.
Further, in the sterilizing agents disclosed in these Patent Documents 1 and 2, in order to improve the sterilizing effect as compared with the combination of only two types, a wider variety of substances are further mixed and the sterilizing effect is high. I was trying to get a disinfectant.

  On the other hand, the present applicant consists only of citric acid, alcohol and electrolyzed water, the citric acid concentration is 0.5 wt% or more, the alcohol concentration is less than 40 wt%, the pH is 3 or less, and the redox potential is An edible disinfectant characterized by being 0 to 300 mV is disclosed (Patent Document 3).

However, the edible disinfectant described in Patent Document 3 contains a small amount of alcohol. From the viewpoint of application to foods, edible sanitizers having a high sanitizing effect without containing alcohol have been desired.
Patent Document 4 discloses a method for sterilizing bacterial spores in which a solution of a dimer bis quaternary ammonium salt to which an edible acid is added is heated and brought into contact with an object to be sterilized.
However, these disinfectants are not sufficient in terms of disinfecting effects and are not excellent in portability because they are provided in a liquid state.

JP-A-8-289768 Japanese Patent Laid-Open No. 11-246312 Japanese Patent No. 4594159 Japanese Patent Laid-Open No. 10-295785

  The present invention has been made in view of the current state of the prior art, and provides an edible disinfectant and an edible disinfectant set that are excellent in portability and have a high disinfecting effect without containing alcohol. With the goal.

  The invention according to claim 1 is a powder or tablet edible disinfectant comprising an organic acid, powdered lactic acid and quaternary ammonium chloride, wherein the organic acid is citric acid, sodium citrate, iron citrate One or more selected from the group consisting of sodium, tartaric acid, lactic acid, malic acid and adipic acid, and the powdered lactic acid is one or more selected from the group consisting of lactic acid, calcium lactate and iron lactate and fine silicon dioxide It is related with the edible sanitizer of the powder or tablet characterized by consisting of these.

  The invention according to claim 2 is a powder or tablet edible sanitizer according to claim 1, a housing part for housing an aqueous solution of the edible sanitizer, and the edible sanitizer in the housing part. The present invention relates to an edible disinfectant set comprising a container having a nozzle part capable of sucking up and spraying an aqueous solution of the agent.

  The invention according to claim 3 relates to an edible disinfectant of an aqueous solution comprising the powder or tablet edible disinfectant according to claim 1 at a concentration of 0.2 to 5 wt / v%.

According to the first aspect of the present invention, there is provided an edible disinfectant of powder or tablet comprising an organic acid, powdered lactic acid and quaternary ammonium chloride, wherein the organic acid is citric acid, sodium citrate, citric acid. It consists of one or more selected from the group consisting of sodium iron oxide, tartaric acid, lactic acid, malic acid and adipic acid, and the powdered lactic acid is one or more selected from the group consisting of lactic acid, calcium lactate and iron lactate and fine particles By using an edible disinfectant of powder or tablet characterized by comprising silicon dioxide, a very high disinfecting effect can be achieved without containing alcohol. In addition to the sterilizing effect, it also has a deodorizing effect.
Since it does not contain alcohol, it can be a safe edible disinfectant even if added to food without impairing the flavor of the food.
By adding fine silicon dioxide to at least one selected from the group consisting of lactic acid, calcium lactate and iron lactate, fine silicon dioxide can be impregnated with fine silicon dioxide to form powdered lactic acid. Thereby, it is not necessary to store and carry in the state of liquid with a large weight and volume, and it can be set as the edible sanitizer excellent in portability.
By taking this structure, it can be set as the disinfectant which does not raise | generate rust easily. Moreover, since it is easy to become a foam, it can be easily made into a foam by using a foam container, and has an advantage that it is easy to use because it remains on the hand particularly when the hand is sterilized.
Quaternary ammonium chloride adversely affects the human body when used in large amounts, but this toxicity can be reduced when used in combination with organic acids.

  According to the invention which concerns on Claim 2, the edible sanitizer of the powder or tablet of Claim 1, the accommodating part for accommodating the aqueous solution of the said edible germicidal agent, and the said edible property in the said accommodating part By being an edible disinfectant set consisting of a container equipped with a nozzle part that can suck up and spray an aqueous disinfectant solution, the edible disinfectant can be easily sprayed as long as there is water. The product can be sterilized.

  According to the invention which concerns on Claim 3, it contains the edible sanitizer of the powder or tablet of Claim 1 by the density | concentration of 0.2-5 wt / v%, The edible sanitizer of the aqueous solution characterized by the above-mentioned. By being present, it is possible to easily sterilize objects such as food and fingers, and to obtain an edible sterilizing agent having a high sterilizing effect.

It is a figure which shows the inactivation effect | action with respect to the feline calicivirus by the various disinfection agent of Example 1, a control, and Comparative Examples 1-17. It is the photograph 24 to 36 hours after the growth medium 30 seconds after contact with the edible sanitizer Escherichia coli of Example 1. It is the photograph after 24 to 36 hours of the growth medium 30 seconds after contact with the disinfectant Escherichia coli of Comparative Example 18. It is the photograph 24 to 36 hours after the growth medium 30 seconds after contact with the disinfectant Escherichia coli of Comparative Example 19. It is the photograph after 24 to 36 hours of the growth medium 30 seconds after contact with the disinfectant Escherichia coli of Comparative Example 20. It is the photograph after 24-36 hours of the growth medium 30 seconds after contact with the edible sanitizer Escherichia coli of the comparative example 21. It is the photograph after 24 to 36 hours of the growth medium 30 seconds after contact with the edible sanitizer Escherichia coli of Comparative Example 22. It is a photograph 24 to 36 hours after the growth medium 30 seconds after contact with the edible disinfectant Escherichia coli of Comparative Example 23. It is a photograph 24 to 36 hours after the growth medium 30 seconds after contact with the edible disinfectant Escherichia coli of Comparative Example 24.

  Hereinafter, the edible disinfectant and the edible disinfectant set of the present invention will be described.

  The edible disinfectant of the present invention is a powder or tablet edible disinfectant comprising an organic acid, powdered lactic acid and quaternary ammonium chloride.

As the organic acid, citric acid, sodium citrate, sodium iron citrate, tartaric acid, lactic acid, malic acid and adipic acid can be used. The edible disinfectant of the present invention can contain one or more of these organic acids.
Among these, citric acid and sodium citrate are preferably used because of the large amount of hydrogen exchange.
As citric acid, for example, citric acid monohydrate can be used.
As sodium citrate, for example, trisodium citrate can be used.
When the edible disinfectant of the present invention is used by dissolving a powder or tablet edible disinfectant in water, the organic acid is 0.1 to 5 parts by weight, preferably 1 to 5 parts by weight with respect to 100 parts by weight of the aqueous solution. It is preferably adjusted and blended so as to be 5 parts by weight. By setting it as this compounding quantity, the disinfection effect can fully be exhibited.

The powdered lactic acid in the present invention comprises at least one selected from the group consisting of lactic acid, calcium lactate and iron lactate and fine silicon dioxide.
Generally, it is difficult to powder lactic acid, but by adding fine silicon dioxide to at least one selected from the group consisting of lactic acid, calcium lactate and iron lactate, lactic acid and / or lactate can be added. Fine silicon dioxide can be impregnated and powdered lactic acid.
The blending ratio (weight ratio) of at least one selected from the group consisting of lactic acid, calcium lactate and iron lactate and fine silicon dioxide is preferably adjusted to 95 to 99: 5-1. By setting it as this compounding quantity, it can be set as powdered lactic acid.
When the edible sanitizer of the present invention is used by dissolving a powder or tablet edible sanitizer in water, 100 parts by weight of the aqueous solution is used in the form of powdered lactic acid (single citric acid at this time is 50% of the powdered lactic acid). It is preferably adjusted and blended so as to be 0.1 to 5 parts by weight, preferably 1 to 5 parts by weight. By setting it as this compounding quantity, the disinfection effect can fully be exhibited.
The edible disinfectant of the present invention is used by being dissolved in water at a concentration of 0.2 to 5 wt / v% so that the organic acid and the powdered lactic acid have the above blending amounts.

  In general, when lactic acid is powdered lactic acid, its sterilizing power decreases. The present invention finds that the sterilizing power of powdered lactic acid is exerted by combining powdered lactic acid and organic acid, and further sterilizing effect is further enhanced by adding quaternary ammonium chloride described later. And the present invention has been achieved.

As quaternary ammonium chloride, benzalkonium chloride, benzethonium chloride, cetylpyridinium chloride and its hydrate (n-hexadecylpyridinium chloride) can be used. The edible disinfectant of the present invention can contain one or more of these quaternary ammonium chlorides.
The edible disinfectant of the present invention is preferably blended with quaternary ammonium chloride adjusted to 0.00005 to 0.0035 parts by weight with respect to 100 parts by weight of the edible disinfectant of powder or tablet. . By setting it as this compounding quantity, the disinfection effect can fully be exhibited.
When sterilizing spores, quaternary ammonium chloride is preferably adjusted to 0.001 to 0.0035 parts by weight. By setting it as this compounding quantity, the microbe elimination effect with respect to a spore bacteria can fully be exhibited.
When bacteria other than spore bacteria are sterilized, quaternary ammonium chloride is preferably adjusted to 0.00005 to 0.0002 parts by weight. The sterilization effect can be sufficiently exhibited with this blending amount.

The edible disinfectant of the present invention has a characteristic that it is difficult to rust because it is a powder or tablet edible disinfectant composed of an organic acid, powdered lactic acid and quaternary ammonium chloride as described above.
Moreover, since it is easy to become a foam, it can be easily made into a foam by using a foam container, and has an advantage that it is easy to use because it remains on the hand particularly when the hand is sterilized.
Quaternary ammonium chloride adversely affects the human body when used in large amounts, but this toxicity can be reduced when used in combination with organic acids.

The edible disinfectant of the present invention can contain an excipient.
As the excipient, lactose or maltose can be used. The edible disinfectant of the present invention can contain one or more of these excipients.
The edible disinfectant of the present invention is in the form of a powder or a tablet. By containing this excipient, the dispersibility of the organic acid and the organic compound can be increased, and a tablet can be obtained.
The edible disinfectant of the present invention is preferably blended with 1 to 10 parts by weight or more of excipients per 100 parts by weight of powder or tablet edible disinfectant. By setting it as this compounding quantity, it can be set as the form of a tablet.

  Since the edible disinfectant of the present invention does not contain alcohol, even if it is properly applied to food, the flavor is not impaired and is safe. Moreover, even if it does not contain alcohol, it has a very high sterilization effect.

The present invention also includes an edible sanitizer set comprising the edible sanitizer of the present invention and a container.
The container includes a storage unit for storing an aqueous solution of the edible sterilizing agent, and a nozzle unit capable of sucking up and spraying the edible sterilizing agent aqueous solution in the storage unit.
Thereby, if there is only water, an edible sanitizer can be easily sprayed and the object can be sanitized.

As described above, the edible disinfectant of the present invention is in the form of a powder or a tablet and is used after being dissolved in water. The water used may be tap water. When used, the edible disinfectant of the present invention is used after being dissolved in water at a concentration of 0.2 to 5 wt / v% of the total weight (including water).
The edible disinfectant of the present invention dissolves immediately in water if it is a powder, and dissolves in 5 to 10 minutes if it is a tablet.
This edible sanitizer aqueous solution can be sprayed on objects such as food and fingers. This makes it possible to sterilize the object.
Examples of the sterilization target include human bodies such as food and fingers, vehicles, toilets, garbage, indoor air, and the like. In addition to sterilization of these targets, deodorization can also be achieved.

  The pH of the aqueous solution when dissolved in water in the edible disinfectant of the present invention is preferably 4.5 or less, more preferably 3 or less. By setting the pH within this range, the sterilization effect can be sufficiently exerted.

  The edible disinfectant of the present invention can be dissolved in water used in an air purifier or a humidifier and sprayed into room air. This allows the room air to be sterilized and deodorized.

The edible disinfectant of the present invention has a disinfecting effect on general bacteria and fungi.
Examples of general bacteria with which the edible disinfectant of the present invention has a disinfecting effect include caries (mutans), periodontal pathogen (P. gingivalis), Escherichia coli O157, Staphylococcus aureus, Salmonella, Vibrio parahaemolyticus, Legionella, Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE) and the like can be mentioned.
Examples of fungi that the edible sterilizing agent of the present invention has a sterilizing effect include ringworm fungi (aquatic fungi), environmental bacteria (serratia fungi), filamentous fungi (penicillium), viruses (feline calici, epidemic cold), and the like. It is done.
Therefore, the edible disinfectant of the present invention has a disinfecting effect against bacteria that are not effective in general alcohol preparations.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further more concretely, this invention is not limited to the following Example at all.

  First, 2 g of citric acid (monohydrate), 1 g of trisodium citrate, 1 g of iron lactate, 1 g of calcium lactate, 0.0001 g of n-hexadecylpyridinium chloride, and Sun Malto S (manufactured by Sanwa Starch Kogyo Co., Ltd.) 2 g was mixed to obtain a powder, and 5.2 g of an edible disinfectant (Example 1) as an example of the present invention was prepared. This was dissolved in 100 g of water to obtain a test solution.

<Test 1>
Purified water was used as a control, and the virus infectivity against feline calicivirus, which is an alternative virus for Norovirus, was measured by the method described below for the test solutions of Examples and Control.

Test method 1) Test virus
Feline calicivirus F-9 ATCC VR-782 (feline calicivirus)
2) Cells used CRFK cells (Dainippon Pharmaceutical Co., Ltd.)
3) Medium used (1) Cell growth medium An MEM medium “Nissui” (Nissui Pharmaceutical Co., Ltd.) with 10% fetal calf serum was used.
(2) Cell maintenance medium A medium obtained by adding 2% of fetal calf serum to Eagle MEM medium “Nissui” (Nissui Pharmaceutical Co., Ltd.) was used.
4) Preparation of virus suspension (1) Cell culture Using the cell growth medium, the used cells were cultured in a single layer in a tissue culture flask.
(2) Inoculation of virus After the monolayer culture, the cell growth medium was removed from the flask and inoculated with the test virus. Next, the cell maintenance medium was added and cultured in a carbon dioxide incubator (CO ₂ concentration: 5%) at 37 ° C. ± 1 ° C. for 1 to 5 days.
(3) Preparation of virus suspension After culturing, cell morphology was observed using an inverted phase contrast microscope, and it was confirmed that morphological changes (cytopathic effect) occurred in the cells. Next, the culture solution was centrifuged (3000 r / min, 10 minutes), and the resulting supernatant was used as a virus suspension.
5) Test procedure 0.1 mL of the virus suspension was added to 1 mL of the sample and mixed to obtain a working solution. The mixture was allowed to act at room temperature, and diluted 10-fold with cell maintenance medium after 10 minutes.
In addition, it tested similarly using purified water as a control | contrast, and also measured at the time of a start.
6) Measurement of virus infectivity titer Using the cell growth medium, the used cells were cultured in a monolayer in a tissue culture microplate (96 wells), and then the cell growth medium was removed and 0.1 mL of cell maintenance medium was added. Next, the working solution and control after 1000-fold dilution were serially diluted 10-fold using a cell maintenance medium. 0.1 mL of the diluted solution was inoculated every 4 holes, and cultured in a carbon dioxide incubator (CO ₂ concentration: 5%) at 37 ° C. ± 1 ° C. for 4 to 7 days. After culturing, the presence or absence of cell morphological changes (cytopathic effect) is observed using an inverted phase contrast microscope, the 50% tissue culture infectious dose (TCID ₅₀ ) is calculated by the Reed-Muench method, and the virus per mL of working solution Converted to infectious titer.
The results are shown in Table 1.

From the results in Table 1, it can be seen that the logarithmic value of TCID ₅₀ per 1 ml of the test solution 10 minutes after Example 1 is halved compared to the start time, and is smaller than that of the control.
Therefore, it turns out that the test liquid of Example 1 has the outstanding disinfection effect with respect to norovirus.

<Test 2>
In the same manner as in Test 1, the inactivation action of the test solution of Example 1 against feline calicivirus, which is a substitute virus for Norovirus, was measured.

The results are shown in FIG.
The graphs of the control and Comparative Examples 1 to 17 in FIG. 1 are quoted from the “2007 Survey Report on Norovirus Inactivation Conditions (National Institute of Pharmaceutical Health Sciences)”.
From this result, it can be seen that the test solution of Example 1 has a sterilizing effect comparable to Na hypochlorite (2000 ppm).

<Test 3>
Next, the bacterial solution of the test bacteria described below was added to and mixed with the test solution of Example 1, and the viable cell count was measured after acting at 25 ° C. for 30 seconds.

1) Test bacteria Escherichia coli NBRC 3972
2. Escherichia coli ATCC 43895 (E. coli, O157: H7)
3. Salmonella enteritidis NBRC 3313 (Salmonella)
4). Vibrio parahaemolyticus RIMD 2210100 (Vibrio parahaemolyticus)
5. Legionella pneumophila GIFU 9134 (Legionella)
6). Staphylococcus aureus subsp. Aureus NBRC 12732 (Staphylococcus aureus)
7). Staphylococcus aureus IID 1677 (methicillin-resistant Staphylococcus aureus (MRSA))
8). Pseudomonas aeruginosa NBRC 13275 (Pseudomonas aeruginosa)
9. Enterococcus faecium NCTC 12204 (vancomycin-resistant enterococci (VRE))
2) Test medium NA medium: Ordinary agar medium (Eiken Chemical Co., Ltd.)
SCDA medium: Tryptosoy agar medium (Eiken Chemical Co., Ltd.)
SCDLP medium: SCDLP medium (Nippon Pharmaceutical Co., Ltd.)
SCDLPA medium: SCDLPA agar medium (Nippon Pharmaceutical Co., Ltd.)
3) Preparation of Bacterial Solution Test Bacteria 2, 7, and 8 are NA medium, Test Bacteria 9 is SCDA medium, Test Bacteria 1 and 3-6 are SCDLP medium, 35 ° C. ± 1 ° C. The bacterial cells were suspended in purified water and prepared so that the number of bacteria per ml was 10 ⁶ to 10 ^7, and used as a bacterial solution.
In addition, a preliminary test was conducted in advance to examine a method for measuring the number of viable bacteria.

The results are shown in Table 2. “Sample” in the table refers to the test solution of Example 1.
Preliminary test that the number of viable bacteria can be measured without being affected by the sample by diluting the test solution with SCDLP medium in E. coli (O157: H7), MRSA and VRE 10 times and Pseudomonas aeruginosa 100 times. Confirmed by

From the results in Table 2, it can be seen that in Example 1, the number of viable bacteria after 30 seconds of each test bacterium was significantly reduced compared to the control.
Therefore, it turns out that the test liquid of Example 1 has a very high disinfection effect with respect to each test microbe.

<Test 4>
In the same manner as in the bactericidal effect test 3, the sterilizing effect on E. coli was compared with other sterilizing agents.

Specimen: Test liquid comparison target of Example 1 Comparative example 18: Competitor product J (strong alkaline ionized water, pH 13.1)
Comparative Example 19: Competitor product K (chlorine / NaClO / dilute HCl: 100 ppm, pH 3.5)
Comparative Example 20: Competitor product L (Ag water, pH 7.3)
Comparative Example 21: Competitor product M (Ion Aqua Detergent, pH 13.0)
Comparative Example 22: Alkaline electrolyzed water (pH 12.5)
Comparative Example 23: Competitor product N (alkaline electrolyzed water, pH 12.6)
Comparative Example 24: Sterile pure water (pH 7.3)

2 to 9 are photographs of the culture medium 30 seconds after contact with Escherichia coli in Example 1 and Comparative Examples 18 to 24, respectively.
In the comparative example, it can be seen that Escherichia coli remains, whereas in Example 1, the Escherichia coli has almost disappeared.
Therefore, it can be seen that the test solution of Example 1 has a very high sterilizing effect even when compared with other sterilizing agents.

  The edible disinfectant of the present invention is suitably used for disinfecting human bodies such as food and fingers, as well as vehicles, toilets, garbage, and indoor air. Further, it is preferably used in combination with an air purifier or a humidifier for the purpose of sterilizing room air.

Claims (3)

An edible sanitizer for powder or tablets comprising organic acid, powdered lactic acid and quaternary ammonium chloride,
The organic acid comprises at least one selected from the group consisting of citric acid, sodium citrate, sodium iron citrate, tartaric acid, lactic acid, malic acid and adipic acid,
An edible disinfectant for powders or tablets, wherein the powdered lactic acid comprises one or more selected from the group consisting of lactic acid, calcium lactate and iron lactate and fine silicon dioxide. An edible disinfectant of the powder or tablet of claim 1;
A container comprising a container for accommodating an aqueous solution of the edible sterilizing agent, and a nozzle part capable of sucking up and spraying the edible sterilizing agent aqueous solution in the container;
An edible sanitizer set consisting of   An edible disinfectant of an aqueous solution comprising the edible disinfectant of the powder or tablet according to claim 1 at a concentration of 0.2 to 5 wt / v%.