KR102275781B1 - Composition for sterilization or disinfection for surface of articles and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a composition for surface sterilization and disinfection and a method for manufacturing the same, and more specifically, to a composition for surface sterilization and disinfection and a method for manufacturing the same, wherein the composition significantly improves durability and alleviates skin irritation by including benzalkonium chloride, didecyldimethylammonium chloride, sea bamboo shoot root extracts, phenoxyethanol and polysorbate.

Description

Composition for sterilization or disinfection for surface of articles and manufacturing method thereof

The present invention relates to a composition for surface sterilization and disinfection and a method for manufacturing the same, and more particularly, to a composition for surface sterilization and disinfection with improved durability of sterilization power on the surface of an article and skin irritation of the human body, and a method for manufacturing the same

In order to prevent infection or transmission of diseases, disinfection should be carried out periodically and at an appropriate time while keeping the surroundings clean. Cleaning and disinfection are essential for disease prevention and prevention and must be followed. The purpose of cleaning is to remove organic substances that reduce the efficacy of the disinfectant and to obtain the maximum effect of disinfection, such as not only effectively killing pathogens, but also reducing the amount of disinfectant used by evenly contacting the disinfectant on the contact surface.

With the recent prevalence of highly contagious diseases such as various respiratory diseases, bacterial diseases, foot-and-mouth disease, or avian influenza, direct or indirect infection of microorganisms is minimized by using disinfectants or disinfectants.

The main component of these disinfectants is mainly ethanol, but when ethanol is used for the purpose of disinfection, harmful microorganisms can be removed for a short time, but only a small amount of microorganisms are removed. It contaminates the environment and the surface of the article.

In order to compensate for the above disadvantages, recently, quaternary ammonium preparations such as aldehyde preparations, chlorine preparations, benzalkonium chloride or didecyl dimethyl ammonium chloride are added using disinfectants. Infection of harmful microorganisms is prevented by disinfecting or sterilizing the surrounding environment and the surface of the product.

For example, as a prior art, Patent Registration No. 10-1913718 relates to a composition for sterilization and disinfection containing benzalkonium chloride as an active ingredient, and discloses a technology capable of effectively sterilizing various pathogenic microorganisms even at low concentrations. have.

However, the disinfection composition according to the prior art has the disadvantage that the sterilization power is limited to a short time, so it needs to be improved to have a more continuous sterilization power, and also a quaternary ammonium agent such as benzalkonium chloride or didecyldimethylammonium chloride is continuous It is necessary to reduce skin irritation such as skin troubles that can cause skin irritation when in contact with it and can cause skin troubles when used excessively. Therefore, there is a further demand for a composition for surface sterilization and disinfection with excellent sterilization effect and durability and very low skin irritation.

Accordingly, the present inventors studied the sterilization effect persistence and skin irritation reduction technology of a composition for surface sterilization and disinfection containing a quaternary ammonium agent such as benzalkonium chloride or didecyldimethylammonium chloride, benzalkonium chloride, didecyldimethyl The present invention was completed by confirming that the composition in which ammonium chloride, ethanol, and Hae bamboo shoot root extract can be mixed can improve the durability of the sterilization effect and skin irritation.

Republic of Korea Patent No. 1913718 (Announcement Date 2018. 11. 01)

Accordingly, an object of the present invention is to provide a composition for surface sterilization and disinfection capable of improving the durability of the sterilization effect on the surface of an article and human skin irritation, and a method for manufacturing the same.

Another object of the present invention is to provide a composition for disinfecting and disinfecting a surface, characterized in that the composition disinfects the surface of an article, and a method for preparing the same.

In order to achieve the above object, the composition for surface sterilization and disinfection according to an embodiment of the present invention includes benzalkonium chloride, didecyldimethylammonium chloride, sea bamboo shoot root extract, phenoxyethanol and polysorbate.

In addition, in the composition for surface sterilization and disinfection according to an embodiment of the present invention, the composition comprises 0.2 to 0.4 parts by weight of benzalkonium chloride, 0.2 to 0.6 parts by weight of didecyldimethylammonium chloride, 0.2 to 0.7 parts by weight of sea bamboo shoot root extract. parts, 0.5 parts by weight of phenoxyethanol, and 0.1 to 0.4 parts by weight of polysorbate.

In addition, in the composition for surface sterilization and disinfection according to an embodiment of the present invention, the sea bamboo shoot root extract is characterized in that it is extracted with water.

In addition, in the composition for disinfecting and disinfecting a surface according to an embodiment of the present invention, the composition is characterized in that the surface of the article is disinfected.

The method for preparing a composition for surface sterilization and disinfection according to an embodiment of the present invention comprises the steps of: preparing a sea bamboo shoot root extract obtained by extracting the dried and pulverized sea bamboo shoot root pulverized product with distilled water; preparing a mixture comprising benzalkonium chloride, didecyldimethylammonium chloride, phenoxyethanol and polysorbate; and preparing a composition for surface sterilization and disinfection by adding the sea bamboo shoot root extract to the mixture, wherein the composition comprises 0.4 parts by weight of benzalkonium chloride, 0.4 parts by weight of didecyldimethylammonium chloride, and a root extract of sea bamboo shoots. 0.45 parts by weight, 0.5 parts by weight of phenoxyethanol, and 0.25 parts by weight of polysorbate.

According to the composition for disinfecting and disinfecting surfaces of the present invention and a method for preparing the same, the composition for disinfecting and disinfecting surfaces of the present invention is a mixture of benzalkonium chloride, didecyldimethylammonium chloride, sea bamboo shoot root extract, phenoxyethanol and polysorbate By doing so, there is an excellent effect of remarkably improving the durability of the sterilization effect and skin irritation.

Since the present invention can apply various transformations and can have various embodiments, preferred embodiments will be described in detail.

The composition for surface sterilization and disinfection according to an embodiment of the present invention includes benzalkonium chloride, didecyldimethylammonium chloride, sea bamboo shoot root extract, phenoxyethanol and polysorbate.

Benzalkonium chloride is a nitrogen cationic surfactant belonging to a quaternary ammonium salt, which destroys cell membranes or inhibits cell membrane synthesis to sterilize. When only benzalkonium chloride is used, it is difficult to expect excellent sterilization and disinfection effects. Accordingly, didecyldimethylammonium chloride belonging to a quaternary ammonium salt is mixed with benzalkonium chloride, but the sterilization effect does not last long and there is still a problem in skin irritation. The present invention confirmed that the durability of the sterilization effect and skin irritation can be remarkably improved by mixing the root extract of sea bamboo shoots with benzalkonium chloride and didecyldimethylammonium chloride.

Nypa fruticans Wurmb. Fruit is a perennial plant belonging to the palm family. It is known to contain a large amount of polyphenols, which are essential elements for generating antioxidant enzymes, and grows in tidal flats rich in nutrients such as calcium, potassium, and sulfur. It is also rich in minerals. However, although studies have been conducted on the application of Hae bamboo shoots to anti-inflammatory treatment, there has been no case of applying Haejunsun to improve the persistence of quaternary ammonium sterilization effect and improve skin irritation.

The composition for surface sterilization and disinfection according to the present invention has an excellent effect in that the sea bamboo shoot root extract increases the durability of the sterilization effect of benzalkonium chloride and didecyldimethylammonium chloride and significantly improves skin irritation.

In the composition for surface sterilization and disinfection according to an embodiment of the present invention, the sea bamboo shoot root extract is characterized in that it is extracted with water.

Phenoxyethanol functions as a preservative, polysorbate functions as an emulsifier as a nonionic polyoxyethylene detergent, and polysorbate 80 is preferred.

As described above, a preferred embodiment of the composition for surface sterilization and disinfection according to the present invention described above is preferably 0.2 to 0.4 parts by weight of benzalkonium chloride, 0.2 to 0.6 parts by weight of didecyldimethylammonium chloride, 0.2 to 0.2 parts by weight of a bamboo shoot root extract. to 0.7 parts by weight, 0.5 parts by weight of phenoxyethanol, and 0.1 to 0.4 parts by weight of polysorbate.

In addition, as another embodiment of the present invention, the composition for surface sterilization and disinfection of the above composition can be used by being impregnated in a predetermined support, and the support includes any one or these of cotton wool or woven fabric, non-woven fabric and knitted fabric made of pure cotton. can be a combination of In addition, as embodiments thereof, it is preferable to pack one sheet at a time, or, in the case of two or more sheets, take out one or more sheets through a hole using a zipper bag or a sticker, and seal the sticker again after use. As an example thereof, a conventional wet tissue container and the like may be applied. The composition for surface sterilization and disinfection according to the present invention may be usefully used to disinfect the surface of an article by being impregnated with a tissue or the like.

The method for preparing a composition for surface sterilization and disinfection according to an embodiment of the present invention comprises the steps of: preparing a sea bamboo shoot root extract obtained by extracting the dried and pulverized sea bamboo shoot root pulverized product with distilled water; preparing a mixture comprising benzalkonium chloride, didecyldimethylammonium chloride, phenoxyethanol and polysorbate; and preparing a composition for surface sterilization and disinfection by adding the sea bamboo shoot root extract to the mixture, wherein the composition comprises 0.4 parts by weight of benzalkonium chloride, 0.4 parts by weight of didecyldimethylammonium chloride, and a root extract of sea bamboo shoots. 0.45 parts by weight, 0.5 parts by weight of phenoxyethanol, and 0.25 parts by weight of polysorbate.

Hereinafter, the present invention will be described in more detail through examples, but the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

<Example>

A mixture of 0.4 parts by weight of benzalkonium chloride, 0.4 parts by weight of didecyldimethylammonium chloride, 0.5 parts by weight of phenoxyethanol and 0.25 parts by weight of polysorbate 80 (TW-80) was prepared.

After drying and pulverizing sea bamboo shoot roots to prepare a sea bamboo shoot root extract, 100 g of dried sea bamboo shoot root pulverized product was placed in a flask and extracted with 1,000 g of distilled water at 80° C. for 3 hours. After the obtained extract was filtered through a filter having a pore size of 0.2 μm, it was concentrated to prepare a sea bamboo shoot root extract.

A surface sterilization solution was prepared by adding 0.45 parts by weight of the sea bamboo shoot root extract to the mixture.

<Comparative example>

Comparative Example 1 was carried out in the same manner as in Example, except for didecyldimethylammonium chloride, 0.8 parts by weight of benzalkonium chloride was mixed to prepare a sterilization solution.

Comparative Example 2 was performed in the same manner as in Example, except for benzalkonium chloride, 0.8 parts by weight of didecyldimethylammonium chloride was mixed to prepare a sterilization solution.

Comparative Example 3 was carried out in the same manner as in Example, except for the root extract of Hae-bamboo shoots, and a sterilization solution was prepared.

Comparative Example 4 was performed in the same manner as in Example, except that 0.45 parts by weight of a sea bamboo shoot leaf extract was added instead of the sea bamboo shoot root extract to prepare a sterilization solution.

<Experimental example>

Sterilization Durability Test

In order to evaluate the sterilization persistence, E. coli (Escherichia coli, ATCC 11229), Staphylococcus aureus (Staphylococcus aureus, ATCC 6538) was inoculated by a plate culture method and cultured in a constant temperature bath at 32 ° C. for 2 days.

After adjusting the initial concentration of each cultured bacteria to be 1×10 ⁶ cfu (colony forming unit)/g, the control test solution (sterile physiological saline), the prepared Examples, and Comparative Examples 1 to 4 Sterilization and The disinfecting composition was diluted 10-fold with purified water and then inoculated and treated for 1h, 12h, 24h, 36h, and 48h to measure the sterilization power (%) according to the following formula 1, and the results are shown in Table 1 below.

[Equation 1]

Sterilization power (%) = (A-B)÷A ×100

(A: number of bacteria from the control test solution after treatment for a certain period of time, B: number of bacteria from the test solution after treatment for a certain period of time)

division Sterilization (%) Initial number of bacteria 1 h 12h 24h 36h 48h Example 1×10 ⁶ 99.9 99.9 99.9 99.9 99.8 Comparative Example 1 1×10 ⁶ 96 87 81 75 70 Comparative Example 2 1×10 ⁶ 94 81 73 66 61 Comparative Example 3 1×10 ⁶ 98 93 87 80 75 Comparative Example 4 1×10 ⁶ 98 94 90 86 82

According to the results of Table 1, the sterilizing power of Comparative Examples 1 to 4 is decreased over time, whereas the Example of the present invention shows an excellent effect of maintaining the sterilizing power for a long time up to 48 hours.

skin irritation test

In order to evaluate the safety and irritation to the human body due to long-term or excessive use of the composition for surface sterilization and disinfection, a primary stimulation test on the human body was performed through the human patch test as follows. It was conducted according to the CTFA guidelines [Reference: The Cosmetic, Toility and Fragrance Association Inc Washington, DC, 20036, 1991] targeting 50 healthy adult males and females. The samples of Examples and Comparative Examples 1 to 4 were diluted 10-fold with purified water in a Finn Chamber, and 15 μl of each was added dropwise, which was then placed on the back of the human body as the test site and fixed with tape. After applying the patch for 24 hours, the patch was removed and 4 hours had elapsed, and the skin reaction of the test site was judged and the degree of irritation was measured according to the following criteria, and the results are shown in Table 2 below.

<Judgment Criteria>

0 ; No erythema or unusual symptoms

One ; Slightly redder than around

2 ; Significantly redder than surrounding

3 ; Redness and swelling more severe than the surrounding area

[Equation 2]

Stimulus = [(0×Number of people) + (1×Number of people) + (2×Number of people) + (3×Number of people)] / Number of test subjects

division Judgment result (number of people) stimulus degree 3 2 One 0 Example - - 2 48 0.04 Comparative Example 1 - 4 8 38 0.32 Comparative Example 2 - 4 7 39 0.30 Comparative Example 3 One 8 9 32 0.56 Comparative Example 4 One 7 7 35 0.48

According to the results of Table 2, the Examples of the present invention exhibit an excellent effect of significantly reducing skin irritation than Comparative Examples 1 to 4.

Therefore, the tissue for disinfecting the surface of an article in which the composition for surface disinfection and disinfection according to the present invention is adsorbed onto the nonwoven fabric has an excellent effect of significantly improving the durability of the sterilization effect and skin irritation.

On the other hand, the above detailed description should not be construed as restrictive in all aspects, but should be considered as exemplary. The scope of the present invention should be determined by a reasonable interpretation of the appended claims, and all modifications within the equivalent scope of the present invention are included in the scope of the present invention.

Claims (5)

0.2 to 0.4 parts by weight of benzalkonium chloride, 0.2 to 0.6 parts by weight of didecyldimethylammonium chloride, 0.2 to 0.7 parts by weight of sea bamboo shoot root extract, 0.5 parts by weight of phenoxyethanol and 0.1 to 0.4 parts by weight of polysorbate A composition for surface sterilization and disinfection.
According to claim 1,
The composition comprises 0.4 parts by weight of benzalkonium chloride, 0.4 parts by weight of didecyldimethylammonium chloride, 0.45 parts by weight of sea bamboo shoot root extract, 0.5 parts by weight of phenoxyethanol and 0.25 parts by weight of polysorbate. for composition.
According to claim 1,
The composition for surface sterilization and disinfection, characterized in that the sea bamboo shoot root extract is extracted with water.
According to claim 1,
The composition is a composition for surface sterilization and disinfection, characterized in that for disinfecting the surface of the article.
Preparing a sea bamboo shoot root extract by extracting the pulverized sea bamboo shoot root by drying and pulverizing the sea bamboo shoot root with distilled water;
preparing a mixture containing benzalkonium chloride, didecyldimethylammonium chloride, phenoxyethanol and polysorbate; and
Preparing a composition for surface sterilization and disinfection by adding the sea bamboo shoot root extract to the mixture;
The composition comprises 0.4 parts by weight of benzalkonium chloride, 0.4 parts by weight of didecyldimethylammonium chloride, 0.45 parts by weight of sea bamboo shoot root extract, 0.5 parts by weight of phenoxyethanol and 0.25 parts by weight of polysorbate. A method for preparing a composition.