KR101913718B1 - Compositions for sterilization and disinfection comprising octenidine and benzalconium chloride - Google Patents

Abstract

The present invention provides a composition for sterilization and disinfection comprising octenidine and benzalkonium chloride as an active ingredient.
Since the composition for sterilization and disinfection according to the present invention can effectively sterilize various pathogenic microorganisms such as pseudomonas, cholera, food poisoning bacteria, E. coli, pneumococci and the like even at a low concentration by containing octenidine and benzalkonium chloride as effective ingredients, It is possible to achieve an excellent microbicidal effect by merely spraying the composition into a liquid form and can be effectively used as a disinfectant and a disinfectant in various industrial fields such as medicine, hygiene, dairy or livestock industry.

Description

TECHNICAL FIELD The present invention relates to a composition for sterilization and disinfection comprising octenidine and benzalkonium chloride as an active ingredient. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for sterilization and disinfection,

The present invention relates to a composition for sterilization and disinfection containing octenidine and benzalkonium chloride as an active ingredient.

In recent years, there have been increasing epidemic diseases such as various respiratory diseases (SARS or MERS), bacterial diseases, foot-and-mouth disease or avian influenza, and there has been an increasing concern among people with such diseases. Microorganisms such as fungi are recognized as major enemies of human health.

Conventionally developed antibiotics such as penicillin have been recognized as an epoch-making medical achievement, but fear of microorganisms is spreading as microorganisms showing resistance to such antibiotics are generated. In particular, super bacteria such as MRSA (methicillin-resistant Staphylococcus aureus) that cause secondary infections in hospitals are becoming a big problem.

Accordingly, although a direct or indirect infection of microorganisms is minimized by using a detergent to which a bactericide or an antifungal agent is added, in the case of a detergent using a surfactant, the harmful microorganisms can not be completely removed, The microorganisms can be removed for a short period of time, but only a small amount of microorganisms are removed, and the microorganisms are proliferated again due to low persistence, thereby polluting the surrounding environment.

Recently, a disinfectant having components such as an aldehyde agent, a chlorine agent, a quaternary ammonium agent, etc. has been added, or a polyvinylpyrrolidone-iodine (PVP-I) Disinfecting or disinfecting the surrounding environment with a disinfectant containing components such as triclosan, chlorohexidine and the like to prevent the infection of harmful microorganisms.

In particular, benzalconium chloride, which is a kind of quaternary ammonium salt preparation, has a low toxicity and is safe for people and various animals. Therefore, a variety of products using the benzalconium chloride are produced, but the disinfecting power is somewhat deteriorated. There is a need for research on how to supplement this.

Korean Patent Publication No. 10-2009-0053208 (published on May 27, 2009) Korean Patent Laid-Open No. 10-2011-0060981 (published on June 6, 2011) Korean Patent Publication No. 10-2014-0066904 (Publication date: 2014.06.03)

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in an effort to solve the problems of the prior art as described above, and it is an object of the present invention to provide a method for sterilizing microbes, The present invention relates to a composition for sterilization and disinfection which can be effectively used in various industrial fields such as dairy industry, livestock industry and the like.

In order to accomplish the above object, the present invention provides a composition for sterilization and disinfection comprising octenidine and benzalkonium chloride as an active ingredient.

Also, the composition for sterilization and disinfection is characterized by containing octenidine and benzalkonium chloride at a weight ratio of 1: 1 to 1:10.

In addition, the composition for sterilization and disinfection may further include at least one additive selected from the group consisting of a surfactant, a chelating agent, a pH adjusting agent, an enzyme, a bleaching agent, and a builder.

In addition, the composition for sterilization and disinfection is characterized by sterilizing at least one microorganism selected from the group consisting of pseudomonas, cholera, food poisoning, pneumococcus, and E. coli.

In addition, the composition for sterilization and disinfection is characterized in that the minimum inhibitory concentration of octenidine and benzalkonium chloride to Pseudomonas aeruginosa is 3.5 쨉 g / mL.

Since the composition for sterilization and disinfection according to the present invention can effectively sterilize various pathogenic microorganisms such as pseudomonas, cholera, food poisoning bacteria, E. coli, pneumococci and the like even at a low concentration by containing octenidine and benzalkonium chloride as an effective ingredient, It is possible to achieve an excellent microbicidal effect by merely spraying the composition into a liquid form and can be effectively used as a disinfectant and a disinfectant in various industrial fields such as medicine, hygiene, dairy or livestock industry.

FIG. 1 shows the result of analyzing the sterilizing effect of a comma germ on a diluted material of a sterilizing and disinfecting composition prepared by the method according to the embodiment.
FIG. 2 shows the result of analyzing the minimum inhibitory concentration of each microorganism in the composition for sterilization and disinfection prepared by the method according to the embodiment.
FIG. 3 is a result of calculating the appropriate concentration of sterilization of the composition for sterilization and disinfection produced by the method according to the embodiment.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for sterilization and disinfection comprising octenidine and benzalkonium chloride as an active ingredient.

The benzalkonium chloride is a nitrogen cationic surfactant belonging to the quaternary ammonium salt. It exhibits an effect of destroying the cell membrane or inhibiting the synthesis of the cell membrane and sterilizing the cell membrane. It has the advantage of low safety and low side effects on humans and animals, It is difficult to expect an excellent sterilization and disinfection effect when only benzalkonium chloride is used.

In the present invention, it is possible to prepare a composition for sterilization and disinfection excellent in sterilization and disinfection effect by mixing octanidine with benzalkonium chloride.

Octenidine is known as octenidine dihydrochloride. It is a cationic surfactant having a dihydrochloride structure. It is strongly adsorbed on the cell surface of the anode, and phospholipids and microorganisms It reacts with anionic polysaccharide on the cell wall, interferes with the enzyme system, interferes with cell function and causes leakage of plasma membrane, resulting in inhibition of mitochondrial function and exhibits excellent microbicidal effect and synergy effect with benzalkonium chloride An excellent sterilization and disinfecting effect can be achieved.

In order to achieve the above-mentioned excellent sterilization and disinfecting effects, the sterilizing and disinfecting composition may contain the octenidine and benzalkonium chloride in a weight ratio of 1: 1 to 1:10.

In addition, the composition according to the present invention may contain a surfactant, a chelating agent, a pH adjusting agent, an enzyme, a bleach, a builder, or a mixture thereof as an additive so as to further improve the sterilizing and disinfecting effect by controlling the pH of the composition .

The pH adjusting agent may include organic acids such as formic acid, acetic acid, citric acid, glycolic acid, lactic acid, succinic acid and malic acid, and inorganic acids such as hydrochloric acid, sulfuric acid, phosphoric acid and nitric acid. In addition, the pH of the composition can be controlled by using metal hydroxides such as sodium carbonate, sodium hydroxide, alkaline earth metal hydroxides, carbonates or ethanol amines.

The chelating agent may form an insoluble complex with oxygen, nitrogen or various metal ions and can be inactivated by various metal ions and may be included in the composition to enhance the sterilization and disinfecting effect. Disodium EDTA, trisodium EDTA, sodium pyrophosphate, etidronate, glucuronic acid, gluconic acid, cyclodextrin, cyclodextrin), and the like, and disodium EDTA can be preferably used.

The enzyme includes an enzyme capable of decomposing various components such as protease, lipase, amylase, hydrolase, cellulase and the like to inhibit the metabolism of microorganisms, An enzyme stabilizer such as benzamidine hydrochloride, boric acid and the like may be further added so as to prevent degradation of metabolites and to prevent physical effects of enzymes, inactivation due to oxidation or decomposition, and the like.

The bleaching agent may be exemplified by peroxide such as sodium percarbonate or phthalimido-peroxy-hexanoic acid, peracid salt or perborate salt.

The builder can increase the detergency against various soil components that can be ingested by microorganisms and microorganisms. Representative examples include sodium polyphosphate, sodium carbonate, sodium silicate, sodium borate, and nitrilotriacetic acid salt.

In addition, the composition according to the present invention may further contain a flavoring agent, an antiseptic agent, a dye, a corrosion inhibitor, etc., and commonly used components may be used.

Furthermore, the composition for sterilization and disinfection according to the present invention may be prepared by adding the above-mentioned additives in a large amount depending on the use and conditions, preferably 0.1 to 5% by weight of the additive Can be configured.

The composition for sterilization and disinfection according to the present invention may further contain a cryoprotectant so that the cryoprotectant composition can be stably used even in cold weather at minus 10 ° C or lower. It is possible to effectively prevent various microbial infectious diseases that may occur even in winter with a very low temperature.

The cryoprotectant may be propylene glycol, citric acid anhydrous, glycerin or the like. Propylene glycol which is non-toxic and stable even when exposed to animals or humans can be preferably used, and the total weight of the composition By weight based on the total weight of the composition. If the content of the cryoprotectant is less than 5% by weight, the effect of preventing the cryopreservation is insignificant, and if it exceeds 25% by weight, the physical properties may be changed.

The composition for sterilization and disinfection according to the present invention may be prepared into a liquid form by mixing octenidine and benzalkonium chloride with the above additives and then mixing with a solvent such as water, ethanol, propylene glycol or the like, and the octenidine And the amount of benzalkonium chloride and various additives can be controlled to maximize the effect of sterilization and disinfection.

The composition for sterilization and disinfection according to the present invention as described above can be used for sterilizing and disinfecting various microorganisms and can effectively sterilize and disinfect pseudomonas, cholera, food poisoning bacteria, .

For example, the composition for sterilization and disinfection according to the present invention comprises 0.5 to 5 parts by weight of octenidine, 1 to 10 parts by weight of benzalkonium chloride, 0.01 to 1 part by weight of disodium iodide, 0.05 to 1 part by weight of phosphoric acid, 0.05 To 1 part by weight of sodium hydroxide and 0.05 to 1 part by weight of enzyme to 100 parts by weight of a solvent such as water, ethanol, polypropylene glycol or the like.

Particularly, 1 part by weight of octenidine, 3.3 parts by weight of benzalkonium chloride, 0.03 parts by weight of disodium ditallow, 0.092 part by weight of phosphoric acid, 0.073 part by weight of sodium hydroxide, 0.03 part by weight of protease, 0.03 part by weight of lipase, 0.03 part by weight of amylase , And 0.01 to 1 part by weight of an enzyme is mixed with 100 parts by weight of a solvent such as water, ethanol, polypropylene glycol or the like to prepare a composition for sterilization and disinfection, which is diluted to 5,000 times. Vibrio Comma Inaba and the like can be sterilized. The composition for sterilization and disinfection can effectively sterilize harmful microorganisms even at a low concentration of the above-mentioned octenidine and benzalkonium chloride at a minimum concentration of 3.5 占 퐂 / mL. have.

As described above, the composition for sterilization and disinfection according to the present invention can effectively sterilize a variety of pathogenic microorganisms such as pseudomonas, cholera, food poisoning bacteria, E. coli, pneumococci and the like even at a low concentration by containing octenidine and benzalkonium chloride as effective ingredients Therefore, it is possible to achieve an excellent microbicidal effect by simply spraying the composition into a liquid form, and thus it can be effectively used as a disinfectant and a disinfectant in various industrial fields such as medicine, hygiene, dairy or livestock industry.

Hereinafter, the present invention will be described in more detail with reference to examples.

The embodiments presented are only a concrete example of the present invention and are not intended to limit the scope of the present invention.

<Examples>

100 mL of a composition for sterilization and disinfection was prepared with the composition shown in Table 1 below.

<Experimental Example 1> Analysis of sterilization and disinfection effect

In order to analyze the sterilizing and disinfecting effect of the composition, the composition prepared by the method according to the Example was diluted with water at a weight ratio of 1: 5,000 to 1: 45,000, and the mixture was applied to a Vibrio Comma ( Comma bacillus ) The effect of sterilizing Inaba was analyzed, and the sterilizing effect was recorded by time zone, and the results are shown in Fig. 1 below.

As shown in FIG. 1, when the composition diluted to 5,000 times was used, it was confirmed that the composition had a germicidal effect of killing comma germs even after lapse of 1 to 15 minutes and had an excellent germicidal effect.

<Experimental Example 2> Antimicrobial activity assay

A tube dilution technique was performed to analyze the activity of the antimicrobial agent in the prepared composition.

First, the culture medium to which the composition of Example was added at different concentrations was injected into a test tube, and the test strain was inoculated in the culture medium. The minimum inhibitory concentration (MIC) of microorganisms was analyzed with Chlorhexidine Alexidine and the results were analyzed by observing the turbidity of cultured tubes. 2. Staphylococcus aureus , Escherichia coli , Klebsiella pneumoniae and Pseudomonas aeruginosa were used as test strains and the minimum inhibitory concentration was expressed in units of ㎍ / mL.

As shown in FIG. 2, it was confirmed that the composition prepared by the method according to the Example inhibited the growth of Staphylococcus aureus and E. coli even at a concentration of less than 1 μg / mL. Especially, It was confirmed that even at a low concentration of less than 1/5 of the cytidine and alexidine, the growth of P. aeruginosa can be effectively inhibited and effectively used for P. aeruginosa infection.

&Lt; Experimental Example 3 >

In order to confirm the optimal contained concentration of the prepared composition according to the intended use, sterilization was performed for various purposes by varying the amount of the composition, and the appropriate concentration of sterilization of the composition was calculated. The results are shown in Fig. The calculation of the appropriate concentration was based on the use of phenolic compounds.

As shown in FIG. 3, it was confirmed that most of the prepared compositions had sterilizing activity even when low concentrations of octenidine and benzenesulfonium chloride were contained below 1 ppm. When the concentration of the active ingredient was increased, , Inhibition of the formation of sticky substances in the pool, and disinfection of dairy equipment and tools.

As a result, it was confirmed that the composition for sterilization and disinfection according to the present invention can be used for various purposes.

Claims (5)

Octenidine and Benzalkonium chloride as effective ingredients in a weight ratio of 1: 1 to 1:10. delete The method according to claim 1,
Wherein the composition further comprises at least one additive selected from the group consisting of surfactants, chelating agents, pH adjusting agents, enzymes, bleaches, and builders. The method according to claim 1,
Wherein at least one microorganism selected from the group consisting of Pseudomonas aeruginosa, Pseudomonas aeruginosa, Cholera bacteria, food poisoning bacteria, pneumococcus, and Escherichia coli is sterilized. 5. The method of claim 4,
Wherein the minimum inhibitory concentration of octenidine and benzalkonium chloride to Pseudomonas aeruginosa is 3.5 占 퐂 / mL.

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