KR102229800B1 - Compositions for sterilization and disinfection comprising enzyme and the manufacturing method thereof - Google Patents

Abstract

The present invention relates to a disinfectant composition comprising an enzyme component and a method of preparing the same, and to a medical device and skin disinfectant composition which causes no skin irritation and unpleasant odor, exhibits excellent properties of cleaning blood that is not easily decomposed, and has a low risk of causing corrosion of a medical device that can easily corrode. More specifically, the method of preparing a disinfectant composition comprising an enzyme component comprises the steps of: (a) preparing a first composition by mixing 35 to 45 wt% of purified water and 3 to 3.3 wt% of alkyldimethylbenzylammonium chloride; (b) preparing a second composition by mixing 0.1 to 0.2 wt% of a sodium edetate hydrate and the prepared first composition; (c) preparing a third composition by mixing 0.02 to 0.07 wt% of amylase, 0.05 to 0.15 wt% of lipases, and 0.1 to 0.3 wt% of protease in 15 to 25 wt% of purified water; (d) preparing a fourth composition by mixing the prepared second composition and the prepared third composition and mixing the mixture thereof and 0.001 to 0.002 wt% of a colorant; and (e) adding 0.04 to 0.08 wt% of sodium hydroxide and the remaining amount of purified water to the prepared fourth composition so that the total weight of the composition becomes 100 wt%, and adjusting the pH of the prepared composition to pH 6 to 8 so as to prepare a disinfectant composition comprising an enzyme component.

Description

Compositions for sterilization and disinfection comprising enzyme and the manufacturing method thereof

The present invention relates to a disinfectant composition containing an enzyme component and a method of manufacturing the same, and more particularly, it does not cause skin irritation and unpleasant odor, has excellent cleaning power of blood that is difficult to decompose, and is corrosive to medical devices that are susceptible to corrosion. The present invention relates to a composition for disinfecting medical devices and skin, and a method for producing the same.

In order to safely reprocess used medical devices such as various medical devices, laryngoscope blades, breathing circuits, suction bottles, scalpels, scissors, etc., remove all biological contaminants such as blood, fat, tissue fragments, etc. from medical devices before sterilization or disinfection treatment. It is desirable to remove. Disinfection and cleaning of contaminated medical equipment is a very important procedure because if any residual contaminants remain on the used medical equipment, the next visited patient can be infected in the hospital.

In addition, in the case of blood, once it comes into contact with medical instruments and skin, it may remain without being completely cleaned even after washing with running water or detergent, so disinfectants and detergents that can completely clean contaminated blood are required.

In addition, in the case of medical devices that are reused several times through cleaning and sterilization with at least the 2nd~3rd procedure, corrosion reactions can easily occur, so there is a possibility of infection during patient surgery with invisible dissolved metal ions. . Accordingly, there is a need to develop a disinfectant and cleaning agent that can prevent the corrosion reaction of medical devices.

Conventional hand sanitizers are widely used disinfectants using ethanol, isopropyl alcohol, octenidine, phosphoric acid, propylene glycol, etc., and these components are bacteria or viruses due to destruction of the cell membrane of bacteria or the envelope of viruses, and coagulation of proteins such as enzymes. It is known to have a disinfecting action against. However, when disinfecting using a disinfectant containing these ingredients, it irritates the user's skin, removing each layer and sebaceous film, falling off each layer, and losing the skin barrier function. There may be a possibility of infection by invading the virus.

Accordingly, the present inventors have invented a disinfectant composition having excellent antibacterial and sterilizing power without causing irritation and unpleasant odor to the skin, having excellent blood cleaning power, low risk of corrosion of medical devices.

Korean Patent Application Publication No. 10-2019-0126135 (November 08, 2019)

The present invention was invented to solve the above problems and is to produce a composition for disinfecting skin without skin irritation and unpleasant odor by minimizing chemical components that are harmful to the human body.

In addition, the present invention is to prepare a disinfectant composition suitable for disinfection and cleaning of medical devices for preventing cross-infection in hospitals because it has excellent decomposition power against organic contaminants in order to completely clean blood that is difficult to decompose easily.

In addition, the present invention is to prepare a composition for disinfecting medical devices with a low risk of corrosion of the devices during sterilization for immersion of medical devices.

The disinfectant composition containing the enzyme component is alkyldimethylbenzyl ammonium chloride 3 to 3.3% by weight, sodium edetate hydrate 0.1 to 0.2% by weight, sodium hydroxide 0.05 to 0.1% by weight, amylase 0.02 to 0.07% by weight, lipase 0.05 to 0.15 Consisting of wt%, protase 0.1 to 0.3 wt%, colorant 0.001 to 0.002 wt% and the balance of purified water, the mass ratio of the lipase and the amylase (lipase/amylase) is 1.3 to 5.2, the protease content and the amylase And mixing so that the mass ratio of the total content of the lipase [protase/(amylase+lipase)] is 1.2 to 3.5, thereby preparing a disinfecting composition containing an enzyme component.

More specifically, the pH of the disinfecting composition may be 6 to 8.

In addition, the present invention relates to a method for preparing a disinfecting composition containing an enzyme component.

The method for preparing a disinfectant composition containing the enzyme component comprises: (a) preparing a first composition by mixing 35 to 45% by weight of purified water and 3 to 3.3% by weight of alkyldimethylbenzylammonium chloride, (b) the Preparing a second composition by mixing 0.1 to 0.2% by weight of sodium edetate hydrate in the first composition, (c) 0.02 to 0.07% by weight of amylase in 15 to 25% by weight of purified water, 0.05 to 0.15% by weight of lipase, and The step of preparing a third composition by mixing 0.1 to 0.3% by weight of a protase, (d) mixing the prepared second composition and the prepared third composition, and mixing 0.001 to 0.002% by weight of a colorant to prepare a fourth composition And (e) adding 0.04 to 0.08% by weight of sodium hydroxide and the remaining amount of purified water to the prepared fourth composition so that the total composition is 100% by weight, and the pH is adjusted to 6 to 8 to prepare a disinfecting composition. Is manufactured.

More specifically, the method for preparing a disinfectant composition containing the enzyme component is (a) 3 to 3.3% by weight of alkyldimethylbenzylammonium chloride while adding 35 to 45% by weight of purified water to a warming stirrer and stirring at 30 rpm. Adding and reacting for 10 to 20 minutes to prepare a first composition, (b) adding 0.1 to 0.2 wt% of sodium edetate hydrate to the prepared first composition and reacting at a speed of 20 rpm for 5 to 10 minutes to prepare a second composition Preparing the composition, (c) adding 0.02 to 0.07% by weight of amylase, 0.05 to 0.15% by weight of lipase, and 0.1 to 0.3% by weight of protase to 15 to 25% by weight of purified water at a temperature of 40°C, and adding 25 to 25% by weight at 50 rpm. After reacting for 35 minutes, cooling to room temperature to prepare a third composition, (d) mixing the prepared second composition and the prepared third composition, adding 0.001 to 0.002% by weight of a colorant, and stirring at a speed of 40 rpm. Step of preparing a fourth composition and (e) adding 0.04 to 0.08% by weight of sodium hydroxide and the remaining amount of purified water to the prepared fourth composition to make the total composition 100% by weight, stirring at 50 rpm for 15 to 20 minutes, and pH A disinfectant composition is prepared by adjusting to be 6 to 8.

The disinfecting composition of the present invention has the effect of significantly lowering skin irritation and unpleasant odor by minimizing chemical components that are harmful to the human body.

In addition, the present invention has excellent decomposition power for organic contaminants in order to completely clean blood that is difficult to be easily decomposed, so there is an effect suitable for disinfection and cleaning of medical devices for preventing cross-infection in hospitals.

In addition, the present invention has a safety effect with less damage such as secondary infection because there is little risk of corrosion of the device during sterilization for immersion of medical devices.

Figure 1: Summary results of virus, bacteria, and fungal sterilization tests of the disinfecting composition of the present invention.
Figure 2: Detailed results of virus, bacteria, and fungal sterilization test results of the disinfecting composition of the present invention.
Figure 3: Virus, bacteria, fungi sterilization test report of the composition for disinfection of the present invention.

Hereinafter, a disinfecting composition containing an enzyme component according to the present invention and a method of manufacturing the same will become apparent with reference to the examples described below in detail. However, the present invention is not limited to the embodiments disclosed below, and may be implemented in various different forms, and is provided to completely inform the scope of the invention to those of ordinary skill in the technical field to which the present invention pertains. It becomes. In addition, the present invention should be construed as including all embodiments falling within the scope of the appended claims.

The first step for preparing the disinfecting composition of the present invention is a step of preparing a first composition by mixing purified water and alkyldimethylbenzyl ammonium chloride.

More specifically, in the first composition, 35 to 45% by weight of purified water is added to a warming stirrer, 1.8 to 3.3% by weight of alkyldimethylbenzyl ammonium chloride is added while stirring at 30 rpm, and reacted for 10 to 20 minutes. The composition may be prepared, and it may be more preferable to add 2.2 to 3.3% by weight of alkyldimethylbenzyl ammonium chloride in order to maximize the sterilizing power, blood cleaning power, and mechanism corrosion preventing ability of the disinfecting composition according to the present invention.

Alkyldimethylbenzylammonium chloride (Benzalkonium chloride) is a nitrogen cationic surfactant belonging to the quaternary ammonium salt. It has the advantage of being less safe. It is well soluble in ethanol and acetone, and is not soluble in water, but the aqueous solution is easy to handle, so the aqueous solution is preferred. The pH of the solution is 6.5~8, and the color is colorless or pale yellow. When the solution is shaken, it foams a lot and has a bitter taste.

The next step of preparing the first composition is a step of preparing a second composition by adding sodium edetate hydrate to the prepared first composition.

More specifically, 0.1 to 0.2% by weight of sodium edetate hydrate may be added to the prepared first composition and reacted for 5 to 10 minutes at a speed of 20 rpm to prepare a second composition, and the sterilizing power of the disinfecting composition according to the present invention , It may be more preferable to add 0.12 to 0.17% by weight of sodium edetate hydrate in order to maximize blood cleaning power and anti-corrosion ability.

The sodium edetate hydrate (Ethylenediaminetetraacetic acid, C ₁₀ H ₁₆ N ₂ O ₈ ) is used as a chelating agent and is also used to remove metal ions in water, and because it has a strong affinity with Pb2+ ions, it is a treatment for lead poisoning. It has also been used as. The complex of iron ions is used to supply iron components to plants, and it can prevent edible oil from rotting by removing metal ions, which are catalysts for oxidation reactions in edible oil, and also used as an anticoagulant of blood, a component of a buffer solution. In analytical chemistry, it is mainly used for analysis, separation, and removal of metal ions.

The next step of preparing the second composition is a step of preparing a third composition by adding and mixing amylase, lipase, and protase to purified water.

More specifically, after adding 0.02 to 0.07% by weight of amylase, 0.05 to 0.15% by weight of lipase, and 0.1 to 0.3% by weight of protase to 15 to 25% by weight of purified water at a temperature of 40°C, and reacting for 25 to 35 minutes at a speed of 50 rpm A third composition can be prepared by cooling to room temperature, and in order to maximize the sterilizing power, blood cleaning power, and device corrosion prevention ability of the disinfecting composition according to the present invention, amylase 0.04 to 0.06% by weight, lipase 0.08 to 0.12% by weight and prota It may be more desirable to add 0.15 to 0.25% by weight of the agent.

Further, the mass ratio of the lipase content and the amylase content (lipase/amylase) is 1.3 to 5.2, and the mass ratio of the protease content and the total content of the amylase and the lipase [protase/(amylase+lipase)] is 1.2 to It may be desirable to add it so that it is 3.5, and in order to maximize the sterilization power, blood cleaning power, and device corrosion prevention ability of the disinfecting composition according to the present invention, the mass ratio of the lipase content and the amylase content (lipase/amylase) is 1.5 to 3.2. It may be more preferable to add so that the mass ratio [protase/(amylase+lipase)] of the protease content and the total content of the amylase and the lipase is 1.3 to 2.2.

The protease is an enzyme that degrades proteins, and is an enzyme that makes a mixture of amino acids or peptides. Types include pepsin, peptidase, trypsin, and the like. Serine protease, Cysteine protease, Threonine protease, Aspartic protease, Glutamic protease, Metalloprotease, Asparagine peptide lyase, etc. may be used as the type of protease, but is not limited thereto.

In order to further increase the cleaning power of the disinfectant composition of the present invention, it may be possible to use two types by using one more type of protase, and the content of the two types of protase used does not exceed 0.1 to 0.3% by weight. It may be desirable to use.

The lipase is an enzyme that breaks down fat into fatty acids and monoglycerides. It is found in the interest amount of animals, and in plants, it is found in castor seeds.

The amylase is an enzyme that hydrolyzes starch and catalyzes its decomposition into sugar. Amylase is present in the saliva of mammals such as humans and initiates the chemical process of digestion. As the amylase, α-Amylase, β-Amylase, γ-Amylase, Amylase Isoenzyme, and the like may be used, but are not limited thereto.

In the step of preparing the third composition, in order to further maximize the anti-corrosion effect of the disinfecting composition according to the present invention, a process of further adding and mixing the fruit extract of carob to the third composition may be additionally performed. have.

More specifically, it may be prepared by adding 0.9 to 2.1 parts by weight of the carob fruit extract to the prepared third composition, and it may be more preferable to add 1.3 to 1.9 parts by weight of the carob fruit extract to maximize the anti-corrosion effect. have.

The scientific name of carob is Ceratonia siligua, and it is a species of shrub or tree belonging to the evergreen plant, and is classified as the legume family. It is called Kerup Tree, Kerup Bean, and Savior Bean Tree. Karov Native to southern Europe, North Africa, the Mediterranean Islands, the Levant, and the Middle East of Western Asia to Iran, the Canary Islands and Macaronesia, it grows up to 15 meters (49 feet). Leaves are 10-20 centimeters (3.9-7.9 inches) long, and male trees do not bear fruit. Although carob is widely used in agriculture, it can also be seen growing in the wilderness of the eastern Mediterranean, and it occurs naturally in the west. Carob fruit extract is known to act as an anti-corrosion effect by adsorbing the extract on the southern surface to create a barrier for material and charge transfer.

The carob fruit can be extracted by methods such as ethanol, hot water, or carbon dioxide supercritical extraction method, and extraction by carbon dioxide supercritical extraction method maximizes the effect of preventing corrosion of medical devices without lowering the sterilizing power of the disinfecting composition of the present invention. It can be made, and it can be desirable. The method of extracting using the carbon dioxide supercritical is to extract carbon dioxide using supercritical carbon dioxide by introducing carbon dioxide for 20 to 35 minutes at a pressure of 250 to 310 bar and a temperature of 35 to 45°C after pulverizing the carob fruit. In order to maximize the disinfecting effect of the disinfecting composition according to the present invention, which can be prepared, the neem tree seeds are introduced into carbon dioxide for 25 to 29 minutes at a pressure of 280 to 300 bar and a temperature of 38 to 42°C. It may be more preferable to extract and prepare using supercritical carbon dioxide.

The next step of preparing the third composition is a step of preparing a fourth composition by mixing the prepared second composition and the prepared third composition and adding a colorant.

More specifically, a fourth composition may be prepared by mixing the prepared second composition and the prepared third composition, adding 0.001 to 0.003% by weight of a colorant, and stirring at a speed of 40 rpm, and the colorant is 0.0015 to 0.0025 wt. It may be more desirable to add %.

Blue No. 102 was used in the embodiment of the present invention, but the colorant may be various colorants depending on the purpose, but is not limited thereto.

The next step of preparing the fourth composition is a step of preparing a disinfecting composition by adding sodium hydroxide and purified water to the prepared fourth composition to adjust the pH.

More specifically, 0.04 to 0.08% by weight of sodium hydroxide and the remaining amount of purified water were added to the prepared fourth composition, so that the total composition was 100% by weight, stirred for 15 to 20 minutes at a speed of 50 rpm, and the pH was adjusted to be 6 to 8. In order to minimize skin irritation of the disinfecting composition of the present invention, it may be more preferable to adjust the pH to a neutral pH. The higher the pH of the alkyldimethylbenzyl ammonium chloride, the stronger the sterilizing power, but since the skin irritation becomes stronger, it may be preferable to prepare it in a neutral region of pH 8 or less.

The sodium hydroxide was used as a pH stabilizer. Sodium hydroxide (NaOH) is dissolved in water to make a strong basic aqueous solution. It is widely used in industries such as textiles, food and beverages, and soap, and is the most widely used base in chemical experiments, and is commonly referred to as caustic soda in the industry. Pure hydroxide. It absorbs water vapor and carbon dioxide well from the atmosphere and dissolves well in water regardless of temperature, and a lot of heat is generated at this time.

The disinfectant composition of the present invention may preferably be diluted with a 1% solution (1:100) when disinfecting and washing the skin, and does not need to be washed separately with water.

When disinfecting and washing medical devices, it can be immersed in a 1% solution (1:100 diluted solution) for 4 to 6 minutes and used as it is. When sterilized, the medical devices sterilized and washed with the disinfectant composition of the present invention are rinsed with water and It may be desirable to disinfect and sterilize by autoclaving at 137°C for at least 1 minute.

The disinfectant composition of the present invention can be used for disinfection and cleaning of medical devices and for disinfection and cleaning of the skin, and the medical devices include a Laryngoscope blade, a breathing circuit, a suction bottle, an oxygen container, an artificial respirator, a newborn baby room incubator, and a medical device ( As a primary disinfectant for medical equipment used in medical equipment), endoscopy equipment, dental equipment (jabari tube, silicone tube, vinyl tube, silicone cap, silicone stopper, mass, endoscopic oil), dental equipment, ward, operating room, intensive care unit, etc. Can be used.

Hereinafter, the disinfecting composition of the present invention will be described in detail by the following test examples and preparation examples.

<Test Example 1> General strain and virus bactericidal power test

1) Preparation of test bacteria solution

Colonies of test bacteria seudomonas aeruginosa ATCC 9027, MRSA ATCC 33591, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 13311, VRE ATCC 51299, Candida albicans ATCC 10231, grown on SABLP medium, were collected with platinum and distilled water. A bacteria solution was prepared by using. This was used as a test bacteria solution.

2) Measurement of initial viable cell count

150 µL of the test bacteria solution was added to 3 mL of distilled water and mixed well. Immediately, 0.5 mL of this bacterial mixture was added to 4.5 mL of a neutralizing agent and mixed well. This was diluted 50 times, 100 times, and 200 times. 1 mL of the diluted solution was dispensed into the dish, and about 15 mL of the SABLP medium stored in the warm bath was added to prepare a mixed stone plate. After the mixed stone plate was solidified, it was inverted and aerobic cultured at 35±2° C. for 2 days. Colonies grown on the mixed stone plate were visually counted. This operation was carried out 3 times.

3) Measurement of the number of viable cells after the action of the test substance

Example 1 150 µL of test bacteria solution was added to 3 mL and mixed well. This was taken as a reaction solution. The reaction was carried out at room temperature (23.0±3.0°C). After 30 seconds and 60 seconds, 0.5 mL of the reaction solution was added to 4.5 mL of a neutralizing agent and mixed well. This was made into a 50-fold, 100-fold, and 200-fold dilution solution. 1 mL of the diluted solution was dispensed into the dish, and about 15 mL of the SABLP medium stored in the warm bath was added to prepare a mixed stone plate. After the mixed stone plate was solidified, it was inverted and aerobic cultured at 35±2° C. for 2 days. Colonies grown on the mixed stone plate were visually counted. This operation was carried out 3 times.

4) Calculation of Log Reduction

The commercial logarithmic value and the average value of the initial viable cell count (CFU/mL) were calculated. From this average value and the average value of the logarithmic value of the number of bacteria (CFU/mL) after the action of the test substance at each action time, the log reduction was calculated by the following equation.

Log Reduction=B-C

B: Average value of initial viable cell count (common logarithmic value)

C: Average value of the number of viable cells (common logarithmic value) after the action of each test substance

5) result

As shown in Figs. 1 to 3, it was found that the disinfecting composition of the present invention (Newgen Hizyme disinfectant) has excellent sterilization effect on all of the tested viruses, bacteria and fungi.

<Test Example 2> Evaluation of the composition

1) Evaluation of blood cleaning power of the composition

In order to evaluate the blood cleaning power of the disinfectant composition, blood was applied to the hands, dried for 30 minutes, and the hands were washed using the disinfecting compositions of Examples and Comparative Examples, and then the blood cleaning power of the composition was measured using a luminol reagent.

In more detail, blood from men in their mid-20s was used, and the collected blood was stored in a K2 EDTA tube (Becton Dickinson, 367525, UK). Blood was thoroughly stirred at room temperature for at least 30 minutes using a roller mixer (Wincom.Co.Ltd., KJMR-II, China) before use in the experiment.

1000 ul of the blood was evenly applied to the hands, and after drying the blood for 30 minutes, the hands were washed. When washing the hands, after washing the hands for 10 seconds in running water, the hands were washed with the disinfecting compositions of the Examples and Comparative Examples for 30 seconds, and the hands were washed in running water for 10 seconds to completely wash the composition. After washing the hands, the hands were strongly shaken to remove moisture first. Thereafter, the hands were lightly shaken for about 90 seconds to remove water a second time, and then a sample was collected by rubbing the palm of the hand with a sterile cotton swab moistened with sterile distilled water. The reaction was observed by dropping 2-3 drops of the prepared luminol working reagent on a flat surface with a cotton swab rubbed on the palm of the hand, and was recorded as the following evaluation criteria based on the average value measured five times.

The luminol working solution was prepared according to the Weber manufacturing method, and the equipment was 100-1000 ul micro pipette (Eppendorf, 3120000062, German), and a conical tube (SPL Life sciences, Korea) was used. When taking pictures, a DSLR camera (Nikon, D7000, Japan), a macro lens (Tamron, SP AF 90mm F/2.8 Di MACRO 1:1, Japan), and a copy stand (RPS Lighting, RS-CS920, Australia) were used. The intensity of the luminol reaction was measured using the Adobe Photoshop version 8.0 (Adobe Systems, USA) program. The detergency of the measured blood was evaluated according to the following evaluation criteria, based on the average value of the following ratings.

[grade]

4 points: The blood was completely washed by the composition.

3 points: more than 1/2 of the blood was washed by the composition

2 points: The blood was washed to less than 1/2 by the composition.

1 point: The blood was washed to less than 1/4 by the composition.

[Evaluation standard]

◎: 4.0 points

○: 3.0 or more and less than 4.0

△: 2.0 points or more and less than 3.0 points

×: less than 2.0 points

2) Evaluation of prevention of mechanical corrosion of the composition

In order to evaluate the anti-corrosion ability of the disinfectant composition, medical instruments were immersed in the disinfecting compositions of Examples and Comparative Examples, rinsed in water, and then sterilized 50 times, and then the corrosion characteristics were measured through an electrochemical method.

In more detail, after immersing the surgical blade in the disinfecting compositions of Examples and Comparative Examples for 5 minutes, rinsing for 30 seconds in purified water, sterilization by autoclaving at 135 degrees for 10 minutes was repeated 50 times, and then through anodic polarization test. Corrosion properties were measured.

The surgical blade, which had been treated repeatedly for 50 times, was placed in a reaction tank containing a solution and connected to a Potentiostat (Model 263, EG&G) to conduct an experiment. The cell used two high-density carbon electrodes as auxiliary electrodes and a saturated calomel electrode (SCE) as the counter electrode. The test solution was carried out by simulating in vivo conditions at degassed 37°C and 0.9wt% NaCl, and dissolved oxygen that may exist in the solution was removed by injecting nitrogen gas into the solution at a rate of 10 ml/min for 30 minutes. Then, in order to remove the influence of oxides or impurities generated on the surface, a cathode potential of -600mV was applied and forcibly reduced for 10 minutes, and then maintained at an open circuit potential for 10 minutes. Thereafter, the test was repeated three times while increasing the potential at a scanning speed of 1 mV/sec in the anode direction from a potential low of about -150 mV from the measured corrosion potential. Corrosion characteristics of the instruments exposed to the measured disinfecting composition were evaluated based on the average value of the following ratings, based on the following evaluation criteria.

[grade]

4 points|pieces: The composition had good resistance to appliance corrosion

3 points: The composition had resistance to corrosion of the apparatus.

2 points: The composition was susceptible to instrument corrosion

1 point: The composition was very susceptible to instrument corrosion

[Evaluation standard]

◎: 4.0 points

○: 3.0 or more and less than 4.0

△: 2.0 points or more and less than 3.0 points

×: less than 2.0 points

3) odor sensory evaluation of the composition

Regarding the odor evaluation, the sensory evaluation method using the human sense of smell according to the current laws and regulations was conducted to evaluate the odor of the disinfecting composition.

In more detail, 50 professional panelists according to the criteria of the odor process test method (Notification No. 2007-17, National Institute of Environmental Sciences), 1 ml of the disinfecting composition of the above Examples and Comparative Examples, were applied to their hands under an environment of 25°C. About the smell generated after taking and rubbing the palm and the back of the hand, sensory evaluation was performed based on the following rating, and the following evaluation criteria were evaluated based on the average value of the rating.

[grade]

4 points: The composition does not have any unpleasant odor

3 points: The composition has a fine unpleasant odor.

2 points: The composition has a slight unpleasant odor.

1 point: The composition has a lot of unpleasant odor

[Evaluation standard]

◎: 4.0 points

○: 3.0 or more and less than 4.0

△: 2.0 points or more and less than 3.0 points

×: less than 2.0 points

4) Evaluation of skin irritation of the composition

Skin irritation sensory evaluation was performed to evaluate the skin irritation of the disinfectant composition.

In more detail, 50 professional panelists took 1 ml of the disinfecting composition of the above Examples and Comparative Examples on their hands under an environment of 25°C and rubbed them on the palms and backs of the hands, and the following ratings were given on the irritation to the skin. Based on the sensory evaluation, it evaluated in the following evaluation criteria based on the average value of the rating.

[grade]

4 points: No irritation is felt by the composition

3 points: no irritation is felt by the composition

2 points: I feel irritation by the composition

1 point: Very irritating by the composition

[Evaluation standard]

◎: 4.0 points

○: 3.0 or more and less than 4.0

△: 2.0 points or more and less than 3.0 points

×: less than 2.0 points

As shown in Tables 1 to 4 above, in the evaluation of blood cleansing power, device corrosion preventing capability, composition odor, and skin irritation, the blood cleansing power of the disinfecting compositions of Examples 1 to 4, device corrosion preventing capability, composition odor, and skin irritation. It was confirmed that the disinfecting compositions of Comparative Examples 1 to 23 exhibited more excellent results than blood cleaning power, anti-corrosion ability, composition odor, and skin irritation.

In the evaluation of blood cleansing power of the disinfecting composition, the weight ratio of "protase/(lipase+apylase)" included in the disinfecting composition is in the range of 1.5 to 3.2, and the content (% by weight) of the protease is 0.1 It was found that the blood cleaning power of the included treatment was the best, and in the evaluation of the anti-corrosion ability of the disinfecting composition, the weight ratio of "lipase/amylase" included in the disinfecting composition was in the range of 1.3 to 2.2, and the content of lipase (weight %) was found to be the most excellent in preventing mechanical corrosion of treatments containing 0.05 or more. In the odor evaluation and skin irritation evaluation of the disinfecting composition, it was confirmed that the disinfecting compositions of Examples 1 to 4 of the present invention did not produce an unpleasant odor and did not irritate the skin at all.

Claims (4)

delete delete In the method for producing a disinfectant composition containing an enzyme component,
The method for preparing a disinfecting composition containing the enzyme component
(a) adding 35 to 45% by weight of purified water to a warming stirrer, adding 3 to 3.3% by weight of alkyldimethylbenzylammonium chloride while stirring at 30 rpm, and reacting for 10 to 20 minutes to prepare a first composition;
(b) adding 0.1 to 0.2% by weight of sodium edetate hydrate to the prepared first composition and reacting at a speed of 20 rpm for 5 to 10 minutes to prepare a second composition;
(c) 0.02 to 0.07% by weight of amylase, 0.05 to 0.15% by weight of lipase, and 0.1 to 0.3% by weight of protase were added to 15 to 25% by weight of purified water at a temperature of 40°C, followed by reaction at 50 rpm for 25 to 35 minutes, at room temperature Cooling to prepare a third composition;
(d) preparing a fourth composition by mixing the prepared second composition and the prepared third composition, adding 0.001 to 0.002% by weight of a colorant, and stirring at a speed of 40 rpm; And
(e) Add 0.04 to 0.08% by weight of sodium hydroxide and the remaining amount of purified water to the prepared fourth composition to make the total composition 100% by weight, stir at 50 rpm for 15 to 20 minutes, and adjust the pH to 6 to 8 To prepare a disinfecting composition,
The mass ratio (lipase/amylase) of the lipase content and the amylase content is 1.3 to 5.2,
The mass ratio of the protease content and the total content of the amylase and the lipase [protase/(amylase+lipase)] is in a ratio of 1.2 to 3.5.
The method of claim 3,
When disinfecting and washing medical devices, the disinfecting composition containing the enzyme component is immersed in a 1% disinfectant solution (1:100 diluted solution) for 4 to 6 minutes and used. A method for producing a disinfecting composition containing an enzyme component, characterized in that the apparatus is rinsed with water and then sterilized and sterilized at a temperature of 134 to 137°C by autoclaving.

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