CN113876680A - Plant wet tissue and preparation process thereof - Google Patents

Abstract

The invention discloses a plant wet tissue and a preparation process thereof, wherein the plant wet tissue is prepared from spunlace non-woven fabrics and a preparation solution, and the preparation solution comprises the following raw materials: 15% -20% of dandelion-lophatherum gracile aqueous extract, 0.1% -0.3% of aloe extract, 0.1% -0.5% of lysozyme, 0.2% -1% of glycine, 0.5% -1% of tea tree oil, 0.05% -0.1% of lemon oil, 0.5% -1% of amino acid type surfactant, 0.2% -0.5% of citric acid, 0.2% -0.5% of sodium citrate, 0.1% -0.3% of stevioside and the balance of purified water. The plant wet tissue obtained by the invention combines multiple effects of cleaning, sterilizing, moisturizing and skin-moistening, anti-allergy and the like, is suitable for cleaning the surface of skin, and can safely and effectively kill gram-positive bacteria and gram-negative bacteria such as staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, candida albicans and the like.

Description

Plant wet tissue and preparation process thereof

Technical Field

The invention belongs to the technical field of daily life nursing products, and particularly relates to a plant wet tissue and a preparation process thereof.

Background

The wet tissue is a cleaning product with high use frequency, and particularly under the condition of novel coronavirus epidemic situation, the disinfection wet tissue occupies a large part of cleaning and nursing consumer market. The disinfection wet tissue is a product which is prepared by taking purified water as production water, taking non-woven fabric, dust-free paper or other raw materials as a carrier and adding a proper amount of disinfectant and has the functions of cleaning and disinfection. Compared with common paper towels, the disinfectant wet tissue has stronger detergency and is more comfortable to use.

However, the wet tissue is in a wet state, so that microbial pollution and mildew are easily caused. Manufacturers often add certain preservatives, and the addition of the preservatives easily causes allergic reactions in children and adults, and can cause contact dermatitis when contacting with the oral mucosa of infants and the like for a long time. Secondly, in order to satisfy the disinfection function, the disinfection wet tissues on the market usually use a disinfectant containing chlorine or alcohol, which has a certain bactericidal activity but strong irritation to the human body and causes problems of dry skin, etc., and is not suitable for infants in particular. Therefore, the development of a safe and effective disinfectant wet tissue is urgently needed by consumers.

Disclosure of Invention

The invention aims to solve the defects of the prior art and provides a plant wet tissue and a preparation process thereof.

The invention is implemented by the following technical scheme:

the plant wet tissue is prepared from spunlace non-woven fabrics and a preparation solution, wherein the preparation solution comprises the following raw materials: 15 to 20 percent of traditional Chinese medicine aqueous extract, 0.1 to 0.3 percent of aloe extract, 0.1 to 0.5 percent of lysozyme, 0.2 to 1 percent of glycine, 0.5 to 1 percent of tea tree oil, 0.05 to 0.1 percent of lemon oil, 0.5 to 1 percent of amino acid type surfactant, 0.2 to 0.5 percent of citric acid, 0.2 to 0.5 percent of sodium citrate, 0.1 to 0.3 percent of stevioside and the balance of purified water.

The extraction method of the traditional Chinese medicine water extract comprises the following steps: weighing herba Taraxaci and folium Bambusae at a weight ratio of 1:1, adding water at a material-to-liquid ratio of 1:8-12, slightly boiling, refluxing for 3 times, each time for 1-2 hr, mixing the extractive solutions for 3 times, and filtering to obtain Chinese medicinal water extract.

The amino acid type surfactant comprises N-acyl amino acid and salts thereof, and N-phthalein amino acid and salts thereof.

The lysozyme is egg white lysozyme.

The preparation method of the plant wet tissue comprises the following specific steps:

step (1): weighing the raw material components according to the proportion;

step (2): uniformly mixing and stirring tea tree oil, lemon oil and amino acid type surfactant to obtain a component A;

and (3): mixing Aloe extract, Chinese medicinal water extract, stevioside and purified water, and stirring to obtain component B;

and (4): uniformly mixing and stirring the component A and the component B, adding lysozyme and glycine, and stirring until the lysozyme and the glycine are completely dissolved to obtain a component C;

and (5): adding citric acid and sodium citrate into the component C, and adjusting the pH value to 5-6 to obtain a preparation solution; the skin of a human body is generally weakly acidic, which is beneficial to preventing the invasion of some bacteria, and if the used wet tissue contains too strong alkalinity, the natural barrier on the surface layer of the skin is damaged, the skin is stimulated, and discomfort and even diseases are caused to the human body. The pH of the preparation solution is adjusted to be weakly acidic by citric acid and sodium citrate, so that skin irritation can be avoided;

and (6): and soaking the spunlace non-woven fabric into the prepared solution to ensure that the spunlace non-woven fabric is saturated in adsorption, thus obtaining a finished product.

The main functions of the components are described as follows:

the traditional Chinese medicine water extract: as the main antibacterial agent, the dandelion aqueous extract has broad-spectrum antibacterial and obvious bactericidal effects; the lophatherum gracile aqueous extract has good antibacterial performance on staphylococcus aureus, escherichia coli, hemolytic streptococcus and the like.

Lysozyme: as a synergist, the antibacterial, antiphlogistic and antiviral composite has the functions of resisting bacteria, diminishing inflammation, resisting viruses and the like, and can selectively dissolve the cell wall of gram-positive bacteria; at the same time, the primary antimicrobial is easier to enter; it can also be directly combined with negatively charged viral protein to form double salt with DNA, RNA and apoprotein to inactivate virus. Meanwhile, other tissues are not damaged, and the safety is high.

Glycine: as a synergist, the structure of endotoxin in gram-negative bacteria cell outer membrane lipopolysaccharide can be changed to a certain extent, so that a peptidoglycan layer is looser, the cell morphology is changed, and the cell permeability is increased; can promote lysozyme to pass through the cell outer membrane of gram-negative bacteria (such as Escherichia coli) and reach the peptidoglycan layer at the hydrolysis action site of lysozyme, thereby improving the antibacterial effect of lysozyme on gram-negative bacteria. The lysozyme and the glycine are compounded, so that the antibacterial and antiseptic effects are better.

Aloe extract: has effects of keeping moisture, relieving, killing and inhibiting bacteria, relieving inflammation, relieving skin dryness, protecting against external irritation, and making the product more comfortable.

Tea tree oil: has good moisture retention and certain bactericidal effect, and the 4-terpineol can remove mites and prevent corrosion.

Lemon oil: has fragrant and pleasant smell, and can be used for resisting bacteria, diminishing inflammation and cleaning skin.

Amino acid type surfactant: as an emulsifier, the tea tree oil and the lemon oil can be uniformly dispersed in the water, and the solubility of the tea tree oil and the lemon oil in water is improved; the antibacterial and bactericidal composition has excellent bactericidal and bacteriostatic properties; can mildly remove dirt and grease on the skin surface, does not stimulate the skin, and is suitable for sensitive skin.

Citric acid and sodium citrate: as a buffer, the pH is adjusted to maintain the pH of the system stable.

Stevioside: the natural sweetener can improve the flavor of the wet tissue and improve the use feeling; is beneficial to reducing the viscosity of the preparation liquid, inhibiting the growth of bacteria and prolonging the shelf life of the product.

Purifying water: as a solvent.

Compared with the prior art, the invention has the following beneficial effects: 1. the invention combines multiple functions of cleaning, sterilization, moisturizing and skin care, anti-allergy and the like, is suitable for cleaning the surface of the skin, and can safely and effectively kill gram-positive bacteria and gram-negative bacteria such as staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, candida albicans and the like; 2. the pure natural antibacterial ingredient dandelion-lophatherum gracile aqueous extract and the safe lysozyme-glycine compound antibacterial agent are added into the wet tissue disclosed by the invention to replace disinfectants such as ethanol and chemical preservatives, so that the irritation degree of the skin can be reduced, and the allergic reaction or contact dermatitis and the like are avoided; is nontoxic when being taken orally and is suitable for infants; 3. by adding the aloe extract and the tea tree oil into the formula, the moisture can be preserved and the dryness of the skin can be reduced while inhibiting bacteria.

Detailed Description

The present invention is further illustrated by the following examples, but the scope of the present invention is not limited by the examples.

Example 1

The plant wet tissue is prepared from spunlace non-woven fabrics and a preparation solution, wherein the preparation solution comprises the following raw materials: 15kg of traditional Chinese medicine water extract, 0.1kg of aloe extract, 0.5kg of lysozyme, 1kg of glycine, 1kg of tea tree oil, 0.05kg of lemon oil, 1kg of amino acid type surfactant, 0.2kg of citric acid, 0.2kg of sodium citrate, 0.1kg of stevioside and 80.85kg of purified water.

The extraction method of the traditional Chinese medicine water extract comprises the following steps: weighing herba Taraxaci and folium Bambusae at a weight ratio of 1:1, adding water at a material-to-liquid ratio of 1:8, slightly boiling and refluxing for 3 times, each time for 1 hr, mixing the extractive solutions for 3 times, and filtering to obtain Chinese medicinal water extract.

The amino acid type surfactant comprises N-acyl amino acid and salts thereof, and N-phthalein amino acid and salts thereof.

The lysozyme is egg white lysozyme.

The preparation method of the plant wet tissue comprises the following specific steps:

step (1): weighing the raw material components according to the proportion;

step (2): uniformly mixing and stirring tea tree oil, lemon oil and amino acid type surfactant to obtain a component A;

and (3): mixing Aloe extract, Chinese medicinal water extract, stevioside and purified water, and stirring to obtain component B;

and (4): uniformly mixing and stirring the component A and the component B, adding lysozyme and glycine, and stirring until the lysozyme and the glycine are completely dissolved to obtain a component C;

and (5): adding citric acid and sodium citrate into the component C, and adjusting the pH value to 5-6 to obtain a preparation solution;

and (6): and soaking the spunlace non-woven fabric into the prepared solution to ensure that the spunlace non-woven fabric is saturated in adsorption, thus obtaining a finished product.

Example 2

The plant wet tissue is prepared from spunlace non-woven fabrics and a preparation solution, wherein the preparation solution comprises the following raw materials: 17.5kg of traditional Chinese medicine water extract, 0.3kg of aloe extract, 0.3kg of lysozyme, 0.6kg of glycine, 0.5kg of tea tree oil, 0.075kg of lemon oil, 0.5kg of amino acid type surfactant, 0.35kg of citric acid, 0.35kg of sodium citrate, 0.2kg of stevioside and 79.325kg of purified water.

The extraction method of the traditional Chinese medicine water extract comprises the following steps: weighing herba Taraxaci and folium Bambusae at a weight ratio of 1:1, adding water at a material-to-liquid ratio of 1:12, slightly boiling and refluxing for 3 times, each time for 2 hr, mixing the extractive solutions for 3 times, and filtering to obtain Chinese medicinal water extract.

The rest is the same as example 1.

Example 3

The plant wet tissue is prepared from spunlace non-woven fabrics and a preparation solution, wherein the preparation solution comprises the following raw materials: 20kg of traditional Chinese medicine water extract, 0.2kg of aloe extract, 0.1kg of lysozyme, 0.2kg of glycine, 0.75kg of tea tree oil, 0.1kg of lemon oil, 0.75kg of amino acid type surfactant, 0.5kg of citric acid, 0.5kg of sodium citrate, 0.3kg of stevioside and 76.6kg of purified water.

The extraction method of the traditional Chinese medicine water extract comprises the following steps: weighing herba Taraxaci and folium Bambusae at a weight ratio of 1:1, adding water at a material-to-liquid ratio of 1:10, slightly boiling and refluxing for 3 times, each time for 1.5 hr, mixing the extractive solutions for 3 times, and filtering to obtain Chinese medicinal water extract.

The rest is the same as example 1.

1. One complete skin irritation test

In 24h before the test, hair on both sides of the spine of the guinea pig was removed with depilatory without damaging the skin. The unhairing range is as follows: the left and right are about 3cm by 3cm, respectively. The next day, 0.5ml of the preparation liquid is directly dripped on the unhaired complete skin on one side with the area of 2.5cm multiplied by 2.5cm, and then covered by a layer of non-irritant plastic film and fixed by a non-irritant adhesive plaster. The other side had hair removed as a blank. The application was carried out for 4 hours, and after the completion of the test, the remaining preparation solution was removed with warm water. Then the local skin reactions were observed at 1h, 24h and 48h after washing the formulation, respectively.

The results show that: the skin of the tested guinea pig has no erythema or edema, and the highest integral of one-time complete skin irritation reaction is '0', which is nonirritating.

2. Test of bacteriostatic Effect

The main antibacterial agent (dandelion-lophatherum gracile aqueous extract), the synergist (lysozyme-glycine) and the preparation liquid are subjected to a comparative test of antibacterial effect, and the specific test method comprises the following steps:

the bacteria content is 1 × 10⁵-1×10⁶cfu/ml bacterial suspension (staphylococcus aureus, escherichia coli and pseudomonas aeruginosa from China general microbiological culture collection center) is evenly smeared on the surface of a culture medium for 3 times, sterile paper sheets dripped with the main antibacterial agent (dandelion-lophatherum gracile aqueous extract), the synergist (lysozyme-glycine) and the preparation solution are respectively stuck on the surface of the culture medium, 3 plates are arranged in each group, 1 negative control is carried out, the culture is carried out for 18 hours at 37 ℃, the diameter (unit: mm) of the bacteriostatic ring is measured, and the comparison results of the bacteriostatic effects of the bacteriostatic agents with different components are shown in the following table 1:

TABLE 1

Group of	Staphylococcus aureus	Escherichia coli	Pseudomonas aeruginosa
				Primary antimicrobial agents	16.25±0.38	17.01±0.67	16.65±0.72
Synergist	11.12±0.21	12.57±0.91	11.54±0.69
				Preparation liquid	21.08±0.73	23.12±0.62	24.43±0.81

The results show that: the diameters of antibacterial rings of the prepared solution, the main antibacterial agent (dandelion-lophatherum gracile aqueous extract) and the synergist (lysozyme-glycine) are all larger than 7mm, which shows that the antibacterial agents have the antibacterial effect. But the bacteriostatic effect of the compound of the main antibacterial agent and the synergist is obviously higher than that of the single main antibacterial agent and the synergist.

3. Stability test

The wet tissue is packaged and then placed in a constant temperature and humidity box (the temperature is 40 +/-2 ℃, and the humidity is 75% +/-5%) to carry out an accelerated test for 3 months, and the test result of the bacteriostasis rate is shown in table 2:

TABLE 2

Bacterial strain	Bacteriostasis rate (month 0)	Bacteriostasis rate (month 3)
			Staphylococcus aureus	99.999％	99.999％
Escherichia coli	99.999％	99.999％
			Pseudomonas aeruginosa	99.999％	97％

As can be seen from Table 2, after the wet tissue provided by the invention is subjected to an accelerated test for 3 months, the bacteriostatic rate of the wet tissue on staphylococcus aureus and escherichia coli is still 99.999%, and the bacteriostatic rate on pseudomonas aeruginosa is slightly reduced, but the requirement is still met. The invention can still maintain the bacteriostatic activity of the product without adding chemical preservatives.

Claims (5)

1. The plant wet tissue is prepared from spunlace non-woven fabrics and a preparation solution, and is characterized in that the preparation solution comprises the following raw materials: 15 to 20 percent of traditional Chinese medicine aqueous extract, 0.1 to 0.3 percent of aloe extract, 0.1 to 0.5 percent of lysozyme, 0.2 to 1 percent of glycine, 0.5 to 1 percent of tea tree oil, 0.05 to 0.1 percent of lemon oil, 0.5 to 1 percent of amino acid type surfactant, 0.2 to 0.5 percent of citric acid, 0.2 to 0.5 percent of sodium citrate, 0.1 to 0.3 percent of stevioside and the balance of purified water.

2. The plant wet tissue as claimed in claim 1, wherein the extraction method of the traditional Chinese medicine aqueous extract comprises the following steps: weighing dandelion and lophatherum gracile according to the weight ratio of 1:1, adding water according to the material-liquid ratio of 1:8-12, slightly boiling and refluxing for 3 times, each time for 1-2h, combining the extracting solutions for 3 times, and filtering to obtain the traditional Chinese medicine water extract.

3. The plant wet tissue as claimed in claim 1, wherein the lysozyme is egg white lysozyme.

4. The wet plant tissue as claimed in claim 1, wherein the amino acid type surfactant comprises N-acyl amino acid and its salts, N-phthalyl amino acid and its salts.

5. The method for preparing the plant wet tissue as claimed in claim 1, which is characterized by comprising the following specific steps:

step (1): weighing the raw material components according to the proportion;

step (2): uniformly mixing and stirring tea tree oil, lemon oil and amino acid type surfactant to obtain a component A;

and (3): mixing Aloe extract, Chinese medicinal water extract, stevioside and purified water, and stirring to obtain component B;

and (4): uniformly mixing and stirring the component A and the component B, adding lysozyme and glycine, and stirring until the lysozyme and the glycine are completely dissolved to obtain a component C;

and (5): adding citric acid and sodium citrate into the component C, and adjusting the pH value to 5-6 to obtain a preparation solution;

and (6): and soaking the spunlace non-woven fabric into the prepared solution to ensure that the spunlace non-woven fabric is saturated in adsorption, thus obtaining a finished product.