CN113424839A - Biological enzyme composite disinfectant and preparation method and application thereof - Google Patents
Biological enzyme composite disinfectant and preparation method and application thereof Download PDFInfo
- Publication number
- CN113424839A CN113424839A CN202110735586.6A CN202110735586A CN113424839A CN 113424839 A CN113424839 A CN 113424839A CN 202110735586 A CN202110735586 A CN 202110735586A CN 113424839 A CN113424839 A CN 113424839A
- Authority
- CN
- China
- Prior art keywords
- parts
- disinfectant
- perfume
- plant
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a biological enzyme composite disinfectant, a preparation method and application thereof, wherein loquat leaves, bamboo leaves, dandelion and houttuynia cordata are dissolved in deionized water, heated to 100 ℃, boiled for 30-60 min and cooled; sequentially adding lysozyme and a plant extracting solution into the cooled water, and magnetically stirring for 1-2 hours until the solution is uniformly mixed; adding spice as required, stirring, and packaging. The biological disinfectant has good antibacterial and bactericidal effects, the antibacterial and bactericidal rate reaches 99.9%, and the preparation process is relatively simple and easy to operate.
Description
Technical Field
The invention relates to the field of disinfectants for daily use, in particular to a biological enzyme composite disinfectant, and a preparation method and application thereof.
Background
The basic task of disinfection is to kill and control harmful microorganisms in the external environment, which is an important measure for effectively cutting off the transmission path of infectious diseases, but the disinfection often causes toxic and side effects and environmental pollution, and the problem makes people start to recognize that the effect of the disinfectant is better than that of a one-handle double-edged sword. Therefore, the disinfection industry is beginning to rank as an important research direction on how to solve the problem, i.e., searching and developing a novel disinfectant or disinfection method which has good sterilization effect but little harm to the environment or no pollution.
The common physical disinfection mainly comprises heat, ultraviolet rays, ultrasonic waves, microwaves, ionizing radiation and the like, and most of the common physical disinfection requires a special generating device. Chemical disinfection is to kill pathogenic microorganisms by using chemical drugs, although the chemical disinfectant has a wide bactericidal spectrum, the chemical disinfectant generally has disinfectant residues which harm common physical disinfection mainly comprises heat, ultraviolet rays, ultrasonic waves, microwaves, ionizing radiation and the like, and a special generating device is mostly needed. Chemical disinfection is to kill pathogenic microorganisms by using chemical drugs, and although the chemical disinfectant has a wide bactericidal spectrum, the chemical disinfectant generally has the harm of disinfectant residues after disinfection. The biological disinfection method is to directly use some substances generated by organisms or indirectly use extracts of the organisms to kill pathogenic microorganisms. The biological disinfectant mainly comprises a disinfectant which takes biological enzyme, bacteriophage, plant extract, antibacterial peptide and the like as main components, can inhibit or kill pests, but cannot generate continuous destructive effect on the environment, and has the defects that the traditional biological disinfection technology generally directly uses organisms for disinfection, the action process is slow, and some problems exist in practical application, such as: the killing effect on the harmful organisms is difficult to determine, the disinfection efficiency is not high, and the large-scale application is not facilitated.
Therefore, in order to search for a safe, low-toxicity, high-efficiency and broad-spectrum disinfectant, the research on the disinfectant in recent years at home and abroad mainly focuses on the aspects: such as developing a new physical method, researching a compounding technology of the disinfectant, utilizing the synergistic effect of physical and chemical factors, developing a preparation technology and an application technology, researching a new disinfection mechanism and the like.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a biological enzyme composite disinfectant product.
Yet another object of the present invention is to: provides a preparation method of the biological enzyme composite disinfectant.
Yet another object of the present invention is to: applications of the above products are provided.
The invention aims to realize the following scheme that the biological enzyme composite disinfectant comprises the following ingredients in parts by mass:
4-6 parts of loquat leaves
3-5 parts of bamboo leaves
2-4 parts of dandelion
1-2 parts of houttuynia cordata
5-8 parts of lysozyme
3-5 parts of plant extract
0.1 to 0.3 portion of spice
Adding the deionized water to 100 parts by weight,
wherein the plant extract is one or a combination of radix tetrastigme extract and pseudo-ginseng plant extract.
Further, the perfume is one or a combination of lavender perfume, jasmine perfume or rose perfume.
The invention also provides a preparation method of the biological enzyme composite disinfectant, which comprises the following steps:
1) dissolving loquat leaves, bamboo leaves, dandelion and houttuynia cordata in deionized water, heating to 100 ℃, boiling for 30-60 min, and cooling to obtain natural plant disinfectant and anti-inflammatory water;
2) sequentially adding lysozyme and a plant extracting solution into the naturally-cooled plant disinfecting and anti-inflammatory water, and magnetically stirring for 1-2 hours until the solution is uniformly mixed;
3) adding spice as required, stirring, and packaging.
The invention also provides application of the biological enzyme composite disinfectant in daily chemicals.
The product of the invention adopts the following method to test the antibacterial performance, comprising the following steps:
1. bacteria passage (Escherichia coli and Staphylococcus aureus pass on a shaker at 37 ℃ and Candida albicans pass on a shaker at 25 ℃);
activating (2), separating (3) and purifying.
2. Preparation of bacterial suspension:
(1) adding 100 microliters of bacterial liquid and 900 microliters of PBS buffer solution into a 1.5 milliliter centrifuge tube from the third-generation purification, and shaking up to be marked as No. 1;
(2) then taking 100 microliters of bacterial liquid and 900 microliters of PBS buffer solution from No. 1, adding into a 1.5 milliliter centrifuge tube, shaking up, and marking as No. 2;
(3) then taking 100 microliters of bacterial liquid and 900 microliters of PBS buffer solution from No. 2, adding the bacterial liquid and the PBS buffer solution into a 1.5 milliliter centrifuge tube, and shaking up to be marked as No. 3;
3. preparing a blank sample solution and a contact sample solution:
(1) blank sample solution: 5 ml of PBS is added into a 15 ml centrifuge tube, and 100 microliters of No. 3 bacterial suspension diluted by escherichia coli, staphylococcus aureus and candida albicans is respectively added.
(2) Contacting the sample solution: 5 ml of sample liquid is added into a 15 ml centrifuge tube, and 100 microliters of No. 3 bacterial suspension diluted by escherichia coli, staphylococcus aureus and candida albicans is respectively added.
4. Contact was carried out for 20 minutes in a shaker (shaking contact culture).
5. After the shaking table is contacted for 20 minutes, 0.1 ml of contact sample liquid of escherichia coli, staphylococcus aureus and candida albicans is respectively put into a test tube containing 1 ml of PBS to be fully mixed, then 0.1 ml of contact sample liquid is taken from the mixed liquid, added into the test tube containing 1 ml of PBS to be fully mixed, and 2 dilutions are made.
6. Adding 200 microliters of the two sample solutions into a solid culture medium, coating in a rotating manner, taking out, and placing into a constant-temperature incubator at 37 ℃.
The product of the invention adopts the following sterilization performance test method:
1. the bacteria were passaged (E.coli and S.aureus at 37 ℃ on a shaker, and Candida albicans at 25 ℃ on a shaker).
(1) Activation (2) separation (3) purification
2. Preparation of bacterial suspension:
(1) adding 100 microliters of bacterial liquid and 900 microliters of PBS buffer solution into a 1.5 milliliter centrifuge tube from the third-generation purification, and shaking up to be marked as No. 1;
(2) then taking 100 microliters of bacterial liquid and 900 microliters of PBS buffer solution from No. 1, adding into a 1.5 milliliter centrifuge tube, shaking up, and marking as No. 2;
(3) then taking 100 microliters of bacterial liquid and 900 microliters of PBS buffer solution from No. 2, adding the bacterial liquid and the PBS buffer solution into a 1.5 milliliter centrifuge tube, and shaking up to be marked as No. 3;
3. preparing a blank sample solution and a contact sample solution:
(1) blank sample solution: 5 ml of PBS is added into a 15 ml centrifuge tube, and 100 microliters of No. 3 bacterial suspension diluted by escherichia coli, staphylococcus aureus and candida albicans is respectively added.
(2) Contacting the sample solution: 5 ml of sample liquid is added into a 15 ml centrifuge tube, and 100 microliters of No. 3 bacterial suspension diluted by escherichia coli, staphylococcus aureus and candida albicans is respectively added.
4. Contact in shaker for 20 minutes (shaking contact culture)
5. And respectively adding 5 ml of D-E broth neutralizing agent into the blank sample solution and the contact sample solution after contact, and fully contacting to obtain a mixed solution.
6. After adding sufficient neutralizing agent, respectively adding 0.1 ml of contact sample liquid of escherichia coli, staphylococcus aureus and candida albicans into a test tube containing 1 ml of PBS, then adding 0.1 ml of contact sample liquid from the mixed liquid into the test tube containing 1 ml of PBS, fully shaking up, and performing 2 dilutions.
7. Adding 200 microliters of the two sample solutions into a solid culture medium, coating in a rotating manner, taking out, and placing into a constant-temperature incubator at 37 ℃.
The invention provides a biological enzyme composite disinfectant, which is prepared by compounding biological enzyme and plant extract, combining the biological enzyme and the plant extract, and simultaneously, better playing a role in antibiosis and sterilization by utilizing natural plant disinfection and anti-inflammation water. The preparation process of the product is relatively simple and easy to operate. The antibacterial bactericidal agent acts for 2 minutes, and the antibacterial bactericidal rate is 99.9 percent respectively.
Detailed Description
The present invention is described in detail by the following specific examples, but the scope of the present invention is not limited to these examples.
Example 1
Dissolving folium Eriobotryae, folium Bambusae, herba Taraxaci, and herba Houttuyniae in deionized water, heating to 100 deg.C, boiling for 30 min, and cooling; sequentially adding lysozyme and the radix tetrastigme plant extract into the cooled water, and magnetically stirring for 1 h until the solution is uniformly mixed; adding lavender essence as required, stirring, and packaging.
The formula (parts by mass) of a preparation method of a biological enzyme composite disinfectant is as follows:
loquat leaf 4 parts
Bamboo leaf 5 parts
Dandelion 3 parts
Houttuynia cordata 2 parts
8 parts of lysozyme
Radix tetrastigme plant extract 3 parts
0.2 part of lavender spice
Deionized water was added to 100 parts.
Example 2
Dissolving folium Eriobotryae, folium Bambusae, herba Taraxaci, and herba Houttuyniae in deionized water, heating to 100 deg.C, boiling for 60 min, and cooling; sequentially adding lysozyme and the panax notoginseng green plant extract into the cooled water, and magnetically stirring for 2 hours until the solution is uniformly mixed; adding jasmine perfume as required, stirring, and packaging.
The formula (parts by mass) of a preparation method of a biological enzyme composite disinfectant is as follows:
loquat leaf 6 parts
Bamboo leaf 3 parts
Dandelion 4 parts
1 part of cordate houttuynia
5 portions of lysozyme
5 parts of pseudo-ginseng plant extract
0.3 part of jasmine spice
Deionized water was added to 100 parts.
Example 3
Dissolving folium Eriobotryae, folium Bambusae, herba Taraxaci, and herba Houttuyniae in deionized water, heating to 100 deg.C, boiling for 60 min, and cooling; sequentially adding lysozyme and the radix tetrastigme plant extract into the cooled water, and magnetically stirring for 2 hours until the solution is uniformly mixed; adding rose essence as required, stirring, and packaging.
The formula (parts by mass) of a preparation method of a biological enzyme composite disinfectant is as follows:
loquat leaf 5 parts
4 portions of bamboo leaves
Dandelion 3 parts
Houttuynia cordata 2 parts
6 portions of lysozyme
Radix tetrastigme plant extract 3 parts
0.2 part of rose spice
Deionized water was added to 100 parts.
Claims (4)
1. The biological enzyme composite disinfectant is characterized by comprising the following raw materials in parts by mass:
loquat leaf 4-6 parts
3-5 parts of bamboo leaves
2-4 parts of dandelion
1-2 parts of cordate houttuynia
5-8 parts of lysozyme
3-5 parts of plant extract
0.1 to 0.3 portion of spice
Adding the deionized water to 100 parts by weight,
wherein the plant extract is one or a combination of radix tetrastigme extract and pseudo-ginseng plant extract.
2. The bio-enzyme composite disinfectant as claimed in claim 1, wherein said perfume is one or a combination of lavender perfume, jasmine perfume or rose perfume.
3. A method for preparing the bio-enzyme complex disinfectant according to claim 1 or 2, comprising the steps of,
1) dissolving loquat leaves, bamboo leaves, dandelion and houttuynia cordata in deionized water, heating to 100 ℃, boiling for 30-60 min, and naturally cooling to room temperature to obtain natural plant disinfectant anti-inflammatory water;
2) sequentially adding lysozyme and a plant extracting solution into the cooled natural plant disinfection and inflammation diminishing water, and magnetically stirring for 1-2 hours until the solution is uniformly mixed;
3) adding spice as required, stirring, and packaging.
4. Use of the bio-enzyme complex disinfectant according to claim 1 or 2 in daily chemicals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110735586.6A CN113424839A (en) | 2021-06-30 | 2021-06-30 | Biological enzyme composite disinfectant and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110735586.6A CN113424839A (en) | 2021-06-30 | 2021-06-30 | Biological enzyme composite disinfectant and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113424839A true CN113424839A (en) | 2021-09-24 |
Family
ID=77758219
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110735586.6A Pending CN113424839A (en) | 2021-06-30 | 2021-06-30 | Biological enzyme composite disinfectant and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113424839A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113876680A (en) * | 2021-11-08 | 2022-01-04 | 云南滇凯术源生物科技有限公司 | Plant wet tissue and preparation process thereof |
-
2021
- 2021-06-30 CN CN202110735586.6A patent/CN113424839A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113876680A (en) * | 2021-11-08 | 2022-01-04 | 云南滇凯术源生物科技有限公司 | Plant wet tissue and preparation process thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Shahen et al. | Effect of antibiotic susceptibility and inhibitory activity for the control of growth and survival of microorganisms of extracts of Calendula officinalis | |
| CN104336101A (en) | Air sterilization method for edible mushroom culture room | |
| Li et al. | Stabilizing the bactericidal activity of hydrogen peroxide: a brandnew function of certain chinese herbs | |
| WO2023272797A1 (en) | Method for extracting alpinia oxyphylla miq essential oil for inhibiting listeria monocytogenes, and alpinia oxyphylla miq essential oil | |
| CN113424839A (en) | Biological enzyme composite disinfectant and preparation method and application thereof | |
| CN108684743A (en) | A kind of sheep hurdle air sanitizer and preparation method thereof | |
| Akbar et al. | Comparative in vitro activity of ethanol and hot water extracts of Zanthoxylum armatum to some selective human pathogenic bacterial strains | |
| Omodamiro et al. | Antimicrobial and coagulant property of Moringa oleifera seed in water purification | |
| CN108273106B (en) | Anti-cold medicine incense | |
| CN106109815A (en) | The disinfection solution that a kind of clinical laboratory personnel wash one's hands | |
| CN112980614A (en) | Preparation system of prickly ash degerming soap | |
| Sultana et al. | Antibacterial effect of aqueous neem (Azadirachta indica) leaf extract, crude neem leaf paste, and ceftriaxone against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa | |
| CN112868680A (en) | Composition with sterilization and disinfection effects and use method and application thereof | |
| CN113207912A (en) | Chicken escherichia coli biological disinfectant and preparation and application thereof | |
| CN103655361B (en) | Traditional Chinese medicinal hand lotion | |
| CN110326627B (en) | Air refreshing spray | |
| CN112516237A (en) | Sterilization and deodorization spray for pets and preparation method thereof | |
| CN113170801A (en) | Disinfectant and preparation method and application thereof | |
| Dangana et al. | Preliminary evaluation of antibacterial potential of four common Nigerian plants against isolates of Staphylococcus aureus and Escherichia coli | |
| CN112516028A (en) | Antibacterial composition and application thereof in cleaning products | |
| Ali et al. | Antimicrobial activity of leaves extracted samples from medicinally important Plumeria obtusa | |
| Shirmohammadi et al. | Antibacterial effects of silver nanoparticles produced by Satureja hortensis extract on isolated Bacillus cereus from soil of sistan plain | |
| Rani et al. | Green synthesis of silver nanoparticles from endophytic fungus Aspergillus niger | |
| CN1246044C (en) | Inbalator type air disinfectant | |
| Al-Qurashy | Antibacterial and antifungal activity of the extract of medicinal plant Malva neglecta Wallr |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication |