CN1246044C - Inbalator type air disinfectant - Google Patents
Inbalator type air disinfectant Download PDFInfo
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- CN1246044C CN1246044C CN 200410067078 CN200410067078A CN1246044C CN 1246044 C CN1246044 C CN 1246044C CN 200410067078 CN200410067078 CN 200410067078 CN 200410067078 A CN200410067078 A CN 200410067078A CN 1246044 C CN1246044 C CN 1246044C
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- disinfectant
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- disinfection
- corrosion
- clove oil
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Abstract
The present invention discloses an aerosol type air disinfectant which is composed of clove oil and a solvent which is easy to dissolve in the clove oil, wherein the raw materials of the clove oil of the present invention are easy to obtain, and have no side effect on human bodies. When in use, the air disinfectant can be diluted by solvents of water, enthanol, etc. without needing to be isolated from people. The present invention has favorable disinfection effect and sterilization effect, and the disinfectant which comprises more than 0.5% of clove oil can finish disinfection after acting in the air within two minutes, which accords with the index requirement of a disinfection technical order. The present invention has favorable stability, and the quality shelf life is about one year. In addition, the disinfectant has low corrosion which is similar to that of distilled water, so that corrosion inspection reports prove that the present invention has no corrosion. The present invention is widely suitable for families, schools, offices, kindergartens, hospitals, hotels, restaurants and other public places needing sterilization and disinfection.
Description
Technical field
The present invention relates to a kind of harmless disinfectant, relating to a kind of in particular is the Inbalator type air disinfectant of active component with the natural aromatic plant quintessence oil.
Background technology
Mainly contain following several with natural plants as the example of fungicidal activity component in the prior art:
One, Shi Qiyun etc. is at " Rhizoma Atractylodis compound disinfectant to air in the detection of microorganism killing effect ", " Chinese Journal of Disinfection ", 1995, Vol (12), No.2, the 114-115 page or leaf has been reported a kind ofly based on Chinese herbal medicine Rhizoma Atractylodis, Herba Schizonepetae extracting solution, adds Rhizoma Atractylodis compound recipe air antiseptic that an amount of Oleum Caryophylli, Herba Menthae wet goods aromatic substance make at 1m
3In the experiment cabinet, 240mg/h speed heating evaporation Chinese herbal medicine Rhizoma Atractylodis compound disinfectant is 90 minutes in accordance with regulations, staphylococcus aureus in the air can be killed and die 100%.At 70m
3Indoor have under people's situation, evaporated medicinal liquid 60 minutes by above-mentioned speed, can kill natural bacteria 69.73% in the air; Two, Wang Shufeng etc. are " the experimental observation person who contains Aloe gel compound disinfectant performance ", " Chinese Disinfection magazine ", 2002, Vol (19), No.4, the 221-224 page or leaf, reported a kind of Aloe gel compound disinfectant, by Aloe gel, Sodium tellurite., the fixed disinfectant solution of forming of Flos Lonicerae extractive solution and 0.1% (g/ml) acetic acid is to staphylococcus aureus on the cloth sheet, escherichia coli effect 5 minutes, to Candida albicans effect on the cloth sheet 10 minutes, killing rate all reaches more than 99.90%, the sheet escherichia coli suspension of dying cloth contains calf serum 50% (ml/ml) and still can be killed more than 99.90%, this disinfectant solution stock solution was deposited 14 days in 54 ℃, act on 10 minutes and still Candida albicans on the cloth sheet can be killed 99.92%, soak aluminum with this agent stock solution, copper, carbon steel, stainless steel substrates 72 hours, corrosion rate＜＜0.0047mm/a, its stock solution is to its mouse oral bag LD50＞5000mg/kg, cumulative coefficient K＞5, to rabbit skin, eye irritant reaction integrated value is 0, so, this disinfectant solution preparation has the effect of certain killing microorganisms and character more stable, and metal is belonged to no corrosiveness; Three, Wu Gaofeng etc. is in " a kind of development of new type air sterilization agent ", " PLA's preventive medicine magazine ", 1996, Vol (14), No.1, the 35-37 page or leaf, having reported a kind of is the disinfectant that primary raw material is made with glutaraldehyde, Folium Isatidis extract, Liquor Flos Lonicerae Distillata extract, the killing rate of dialogue staphylococcus, staphylococcus aureus and bacillus pyocyaneus is all about 99.83% in the laboratory; Four, Fan Yanping etc. is in " observation of Chinese and western drugs air sanitizer clinical application effect ", " Shanxi nursing magazine ", 2000, Vol (14), No.3, the 130-131 page or leaf has been reported a kind of Chinese medicine air sanitizer of forming with Radix Scutellariae, Folium Isatidis, Ilex purpurea Hassk.[I.chinensis Sims, Cortex Moutan, Cortex Magnoliae Officinalis, Herba Eupatorii, bright Herba Menthae etc., and the sterilization effective percentage is about 93.75%.To sum up, four kinds of prior art is also to contain harmful chemical substances such as Sodium tellurite., acetic acid are fixed, glutaraldehyde in the air sanitizer of active component or 99.9% or the component that killing rate is lower than the requirement of sterilization rules except that natural plants with the natural plants, therefore Disinfection Effect is not ideal enough, or when sterilization must isolated crowd, it is convenient inadequately to use.The above-mentioned in addition disinfectant Disinfection Effect persistent period is short, abnormal smells from the patient is fragrance inadequately, so the natural perfume smell disinfectant of prior art can not satisfy the regular sterilization requirement in family, school, office, kindergarten, hospital, hotel, restaurant and other public place.
Summary of the invention
It is the Inbalator type air disinfectant of active component with natural aromatic plant quintessence oil Oleum Caryophylli (Syringa vulgaris L.) that technical problem to be solved by this invention provides a kind of, this disinfectant is free from side effects to human body, must isolated crowd during use, raw material is easy to get, easy to use, cost is low, has good sterilization, bactericidal effect.
The technical solution adopted in the present invention: a kind of Inbalator type air disinfectant by Oleum Caryophylli and the solvent composition be made up of water, ethanol or their mixture, is characterized in that the content of described Oleum Caryophylli in solvent is weight percentage 0.5～2.0%.
Described Oleum Caryophylli is the commodity Oleum Caryophylli that fragrant biotechnology company in sky, Kunming, Yunnan or positive element spices and essence company of Shanghai Beijing University sell.
The invention has the beneficial effects as follows: the invention provides a kind of is the Inbalator type air disinfectant of active component with natural aromatic plant quintessence oil Oleum Caryophylli (Syringa vulgaris L.), this disinfectant is free from side effects to human body, get final product in direct dilution and water, the ethanol equal solvent during use, can make aerosol easily and make it to be suspended in the air, must isolated crowd during use.Natural aromatic plant quintessence oil Oleum Caryophylli raw material is easy to get, easy to use, cost is low.Contain 0.5% or the disinfectant of above concentration Oleum Caryophylli to the index bacterium in " disinfection technology standard ", reached 99.9% as staphylococcus aureus, colibacillary killing rate, meet " disinfection technology standard " specification requirement to disinfectant.Active component and solvent among the present invention generally have no side effect to human body.The present invention also has good stable, and the shelf life of its product had at least about 1 year.In addition, corrosivity of the present invention is lower than peracetic acid disinfectant commonly used 1%, and is about the same with distilled water.The present invention can be widely used in family, school, office, kindergarten, hospital, hotel, restaurant and the sterilization of other public place, germ-resistant needs because of possessing above-mentioned advantage.
The specific embodiment
Below in conjunction with specific embodiment the present invention is described in further detail: a kind of Inbalator type air disinfectant, by Oleum Caryophylli and the solvent composition that is easy to dissolve Oleum Caryophylli.Described solvent is water, ethanol or their mixture.The content of described Oleum Caryophylli in solvent is weight percentage 0.5%～2.0%.Because active component Oleum Caryophylli among the present invention and water or ethanol well dissolve each other, so disinfectant can be made aerosol easily and makes it to be suspended in the air.
Embodiment 1
Oleum Caryophylli to the test inhibitory potency (MIC) of bacterium and sterilization tire (MBC) test.Described Oleum Caryophylli is the commodity Oleum Caryophylli that fragrant biotechnology company in sky, Kunming, Yunnan or positive element spices and essence company of Shanghai Beijing University sell.Test is staphylococcus (ATCC 6538 strains), escherichia coli (8099 strain) with antibacterial, and 18 hours agar slant cultures will be tested bacterium and use normal saline corrected concentrations to 0.5 Maxwell than turbid standard, and the dilution in 1: 10 of reuse MH meat soup becomes 1 * 10 with diluent preparing
8The CFU/ml bacterial suspension.Culture medium is: MH agar culture medium, MH fluid medium.And physiological saline solution.Carry out following operation with reference to antibiotic medicine sensitization test tube dilution method:
1, get and contain 10 in 2mlMH fluid medium test tube, two-fold dilution's sample stock solution is to maximum concentration to be measured.
2, getting 0.1ml bacterium liquid with micro sample adding appliance is added in each test tube to high concentration by low concentration successively.
3, the result observes: hatch the lowest concentration of drug that suppresses test bacterial context eye visible growth behind the 24h for 35 ℃ and be the MIC of this medicine to the test mattress.
4, will not see that culture is got an inoculating loop in the bacterial growth test tube, transferred species is in MH agar culture medium (containing nertralizer, sodium thiosulfate 5%, lecithin 0.5%, tween degree-80 3%), 35 ℃ hatch 24h after, observation has or not bacterial growth, to differentiate that antibacterial is suppressed or is killed.
5, quality control:
The meat soup contrast: the MH fluid medium is used in test, does not add any article.
The contrast of test bacteria growing: test adds bacteria tested with the MH fluid medium
The contrast of sample sterility test: sample 1ml adds test MH fluid medium 2ml.
Bacterial growth suppresses contrast: test adds gentamycin with the MH fluid medium, and (final concentration 10 units/ml) add bacteria tested.
Oleum Caryophylli is tired to the test inhibitory potency of bacterium and sterilization and is seen Table 1.
Table 1
| Sample number into spectrum | Inhibitory potency | Sterilization is tired | ||
| Staphylococcus | Escherichia coli | Staphylococcus | Escherichia coli | |
| Oleum Caryophylli | 1∶640 | ＞1∶1280 | 1∶320 | 1∶640 |
Conclusion: Oleum Caryophylli all has certain biocidal property and bactericidal properties to staphylococcus and escherichia coli.
Embodiment 2
Invent described Inbalator type air disinfectant bactericidal effect.Oleum Caryophylli adds the solution for later use that cosolvent is made into percentage by weight 0.5%, 1.0%, 1.5%, 2.0% with ethanol or water.Test is staphylococcus (ATCC 6538 strains), escherichia coli (8099 strain) with antibacterial, and 18 hours agar slant cultures become 1 * 10 with diluent preparing
8The CFU/ml bacterial suspension.Nertralizer 0.5% sodium thiosulfate, 3.0% tween 80,0.5% lecithin.Organic substance disturbs liquid 3% Bovine Serum Albumin in Aqueous Solution.Physiological saline solution.Diluent (normal saline PH7.0 ± 0.2 that contains 0.1% tryptone).Culture medium: Nutrient agar, MH fluid medium.
At first carry out the selection test of following nertralizer: cancellation toxic agent, bacteria suspension, nertralizer etc., as 6 groups of different modes combinations, the effect of test nertralizer, to select qualified nertralizer, combination is respectively:
The 1st group: disinfectant+nertralizer → cultivation;
The 2nd group: (disinfectant+bacteria suspension)+nertralizer → cultivation;
The 3rd group: nertralizer+bacteria suspension → cultivation;
The 4th group: (disinfectant+nertralizer)+bacteria suspension → cultivation;
The 5th group: diluent+bacteria suspension → cultivation;
The 6th group: diluent+nertralizer+culture medium → cultivation.
Cultivating back as the 1st group does not have antibacterial or minority bacterial growth only, and the 2nd group than more than the 1st group, but than 3,4,5 groups of few bacterial growths, the 3rd, 4,5 group has similar bacterial growth then to be qualified nertralizer.See " disinfection technology rules " for details.The nertralizer of different formulations, after measured after, found that the nertralizer that contains 0.5% sodium thiosulfate+3.0% tween 80+0.5% lecithin is effective, play complete neutralization, so in sterilizing test, select for use this prescription nertralizer.Agent bactericidal effect then carries out disinfection:
1, gets the bacterial suspension for preparing, add the Organic substance liquid of equivalent, placed 5 minutes;
2, get the above-mentioned bacterial suspension of 1ml and add the 4ml disinfectant, mix, act on 5,10 minutes;
3, take out 0.5ml and join in the 4.5ml nertralizer, mix, act on 10 minutes;
4, take out, through different dilution factors, pour plate, colony counting;
5, calculate sterilizing rate:
Kill the logarithm value of the logarithm value-test group viable bacteria concentration of logarithm value=matched group viable bacteria concentration.
The disinfectant bactericidal action that contains 2.0% Oleum Caryophylli that different time is measured sees Table 2:
Table 2
| Antibacterial | The sterilizing rate % of different time | ||
| 10 minutes | 5 minutes | 2 minutes | |
| Staphylococcus | 99.9 | 99.9 | 99.9 |
| Escherichia coli | 99.9 | 99.9 | 99.9 |
Conclusion: 2.0% Oleum Caryophylli prescription meets the requirement of disinfection technology rules.
The disinfectant bactericidal action that contains the variable concentrations Oleum Caryophylli sees Table 3:
Table 3
| Antibacterial | Action time | The sterilizing rate % of variable concentrations | ||
| 1.5％ | 1％％ | 0.5％ | ||
| Staphylococcus | 2 | 99.9 | 99.9 | 99.9 |
| Escherichia coli | Minute | 99.9 | 99.9 | 99.9 |
Conclusion: test concentrations is that the Oleum Caryophylli of 0.5%-1.5% all meets the requirement of disinfection technology rules.
The bactericidal action stability test of disinfectant of the present invention the results are shown in Table 4:
Table 4
| Action time | Strain | Bacterium colony forms several cfu/ml | ||
| Specimen (2.0% Oleum Caryophylli) | Contrast | |||
| 2 minutes | Be untreated | 54 ℃ of placements, 14 days | Diluent | |
| Staphylococcus | ＜10 | ＜10 | 2.3×10 7 | |
| Escherichia coli | ＜10 | ＜10 | 1.01×10 7 | |
Conclusion: show that disinfectant of the present invention has good stable.
Embodiment 3
The corrosion testing of disinfectant.Test is that (phosphorus content is 0.05%-0.12% to carbon steel, and density is 7.87g/cm with metal
3), (99.5%, density is 2.71g/cm to industrial blunt aluminum
3), (oxygen-free copper, density are 8.94g/cm to copper
3).The test metal is all made standard specimen, and accurately measures the size (being accurate to 0.001mm) of sample with electronic digital display micrometer, calculates and is subjected to corrosive metal surface area.Test is preceding with 360
#Waterproof abrasive paper grinds off metal testing plate surface oxide layer as thin as a wafer, clean up sample with distilled water, filter paper is dried, after the acetone oil removing, again through 50 ± 1 ℃ of oven dry 1 hour, accurately take by weighing weight (being accurate to 0.1mg) before the test button corrosion with electronic balance, then, every group is soaked in the 300ml disinfectant with 3 identical samples and does corrosion testing.Etching time is 72 hours, test temperature is 28 ± 1 ℃, to the scheduled time, taking out sample cleans up with distilled water, the corrosion product distilled water of carefully removing specimen surface with clean hairbrush cleans, and filter paper is dried, again through 50 ± 1 ℃ of oven dry 1 hour, accurately take by weighing the weight (being accurate to 0.1mg) of test button corrosion front and back, be calculated as follows corrosion of metal speed (the weightless index and the depth index of metal erosion).
V_=(W
0-W
1)/s * t (gram/rice
2* hour) (g/m
2* h)
V_: the weightless index (gram/rice of metal erosion
2* hour) (g/m
2* h)
W
0: the initial weight of metal (gram) is (g)
W
1: the weight (gram) of having removed the metal behind the corrosion product is (g)
S: be subjected to corrosive metal surface area (rice
2) (m
2)
T: etching time (hour) (h)
V
L=V_ * 24 * 365 * 10/ (100)
2* ρ=V_ * 8.76/ ρ (millimeter/year) (mm/a)
V
L: the depth index of metal erosion (millimeter/year) is (mm/a)
ρ: the density (gram per centimeter of metal
3) (g/cm
3)
Corrosion level criterion:
0.001＜V
L≤ 0.01 (uses V for not having corrosion substantially
L0Expression);
0.01＜V
L≤ 0.1 (uses V for mild corrosion
L1Expression);
0.1＜V
L≤ 1.0 (use V for moderate corrosion
L2Expression);
0＜V
L≤ 10 (use V for severe corrosion
L3Expression).
Disinfectant is to the corrosion control experiment result of different metal, sees Table 5, table 6.
Table 5
| Concentration and kind distilled water peracetic acid 1% 2% Oleum Caryophyllis | Different metal corrosion rate (mm/a) | ||
| Carbon steel Carbon steel 0.0636 (V L1) 0.6219(V L2) 0.0466(V L1) | Copper Copper 0.0138 (V L1) 4.3155(V L3) 0.0170(V L1) | Aluminum Aluminum 0.0773 (V L1) 1.0712(V L3) 0.0627(V L1) | |
Table 6
| Concentration and kind distilled water peracetic acid 1% 2% Oleum Caryophyllis | Weightless index (the g/m of metal erosion 2×h) | ||
| Carbon steel Carbon steel 0.0571 0.5587 0.0419 | Copper Copper 0.0141 4.4042 0.0174 | Aluminum Aluminum 0.0239 0.3314 0.0194 | |
Conclusion: the corrosivity of disinfectant of the present invention is lower than peracetic acid 1%, and is similar to distilled water.
Oleum Caryophylli more than 0.5% is dissolved in ethanol or water and makes air sanitizer and act on air and promptly meet more than 2 minutes＜the disinfection technology rules〉index request.Described air sanitizer has good stability, and the quality shelf-life is about 1 year.Described air sanitizer corrosivity is lower than common disinfectant, and is suitable with distilled water substantially, to corrosivity survey report non-corrosiveness.Above said content only is the basic explanation of the present invention under conceiving, and according to any equivalent transformation that technical scheme of the present invention is done, all should belong to protection scope of the present invention.
Claims (1)
1. Inbalator type air disinfectant by Oleum Caryophylli and the solvent composition be made up of water, ethanol or their mixture, is characterized in that the content of described Oleum Caryophylli in solvent is weight percentage 0.5～2.0%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410067078 CN1246044C (en) | 2004-10-12 | 2004-10-12 | Inbalator type air disinfectant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410067078 CN1246044C (en) | 2004-10-12 | 2004-10-12 | Inbalator type air disinfectant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1600374A CN1600374A (en) | 2005-03-30 |
| CN1246044C true CN1246044C (en) | 2006-03-22 |
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ID=34666543
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|---|---|---|---|
| CN 200410067078 Expired - Fee Related CN1246044C (en) | 2004-10-12 | 2004-10-12 | Inbalator type air disinfectant |
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| Country | Link |
|---|---|
| CN (1) | CN1246044C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101711883B (en) * | 2009-10-30 | 2013-01-23 | 浙江绿岛科技有限公司 | Aerosol air purification freshener and preparation method thereof |
| CN113729038A (en) * | 2021-07-13 | 2021-12-03 | 江西省科学院微生物研究所 | Traditional Chinese medicine air disinfectant |
-
2004
- 2004-10-12 CN CN 200410067078 patent/CN1246044C/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN1600374A (en) | 2005-03-30 |
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