CN113729038A - Traditional Chinese medicine air disinfectant - Google Patents
Traditional Chinese medicine air disinfectant Download PDFInfo
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/21—Use of chemical compounds for treating air or the like
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Abstract
The invention provides a traditional Chinese medicine air disinfectant, which consists of the following components in percentage by volume: the compound essential oil comprises 10% of compound essential oil and 90% of solvent, wherein the compound essential oil is oregano essential oil: cinnamon essential oil: litsea cubeba essential oil: citronella essential oil 3: 3: 2: 2, the solvent is dimethyl sulfoxide. The air disinfectant disclosed by the invention is a safe, efficient, environment-friendly, economic and convenient indoor air freshening disinfectant, is suitable for indoor air freshening disinfection under the condition that people smoke in indoor public places, can effectively humidify indoor air and freshen the indoor air when the indoor air is freshened and disinfected, and is particularly suitable for indoor air freshening disinfection in public areas with low air temperature, dry air and tight doors and windows in winter.
Description
Technical Field
The invention belongs to the field of application of traditional Chinese medicine extracts, and relates to a traditional Chinese medicine air disinfectant.
Background
In recent years, air microbial contamination has become more serious. The main sources of microorganisms in the air include soil, dust, animals and plants, water surface, human body and production activities, sewage and dirt treatment and the like, and the main sources of microorganisms in the indoor air include release of some building materials, facility arrangement, human body activities, microorganism self-reproduction, outdoor inflow and the like. Pathogenic microorganisms in the air enter the body through a respiratory system to cause respiratory diseases or infectious diseases such as asthma, SARS, pneumonia, avian influenza, various influenza, tuberculosis, measles, mumps, new coronavirus and the like, thereby seriously threatening the health of people and animals. Air disinfection can cut off the transmission path of respiratory infectious diseases, and is the most effective and direct method for preventing and controlling infection. The currently common physical and chemical air disinfection methods mainly include ultraviolet irradiation, purification laminar flow disinfection, plasma disinfection, air filter disinfection, nano-photocatalytic technology disinfection, fumigation of chemical agents, aerosol spraying and the like. Although the treatment methods can quickly and effectively kill pathogenic microorganisms in indoor air pollution, chemical medicines remained by a plurality of chemical agents become pollutants, and harm to human bodies is caused to different degrees. During disinfection, operators have to leave the field and take protective measures, the retention time of peculiar smell after disinfection is long, and the risk of accumulated poisoning of human bodies can be caused after long-term use. For example, ultraviolet irradiation causes certain damage to human skin and eyes, and ozone is generated to cause ozone pollution. The ultraviolet radiation disinfection effect is not long, the number of bacteria quickly rises after the radiation is stopped, and the lamp light intensity, the temperature, the humidity, the cleanliness, the shelter, the distance and the like can influence the disinfection effect. 84 when the concentration of the disinfectant is higher, the disinfectant has stronger stimulation or damage to mucous membranes and skins of human bodies. Peracetic acid has a high-efficiency, quick-acting and broad-spectrum disinfection effect on bacteria, fungi, viruses and the like, is an ideal air disinfectant, but has high-concentration peracetic acid, large pungent smell, high disinfection risk, large transportation risk and large influence of the environment on the stability of the peracetic acid. Although equivalent dilution to lower concentrations may circumvent these disadvantages, the kill rate may also be reduced. As for plasma disinfection, air filter disinfection, physical purification laminar flow disinfection and the like, the investment is large, and the wide popularization and application of the device in families and public places are difficult. Although these methods can rapidly and efficiently kill harmful microorganisms in indoor air pollution, they are expensive, have strong toxic and side effects, have strong drug resistance, and easily pollute the ecological environment. Therefore, exploring a safe, efficient, economical and environment-friendly efficient air pollution disinfectant, and preventing air pathogenic microorganism pollution are main research subjects of epidemic prevention and air environment pollution treatment, and are also problems to be paid attention to and urgently solved.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the traditional Chinese medicine air disinfectant which can effectively kill microorganisms in the air while removing peculiar smell, refreshing and increasing aroma, thereby improving the air quality.
The invention is realized by adopting the following technical scheme: a traditional Chinese medicine air disinfectant comprises the following components in percentage by volume: the compound essential oil comprises 10% of compound essential oil and 90% of solvent, wherein the compound essential oil is oregano essential oil: cinnamon essential oil: litsea cubeba essential oil: citronella essential oil 3: 3: 2: 2, the solvent is dimethyl sulfoxide.
Preferably, the compound essential oil is oregano essential oil: cinnamon essential oil: litsea cubeba essential oil: clove essential oil 3: 3: 2: 2;
preferably, the compound essential oil is oregano essential oil: cinnamon essential oil: litsea cubeba essential oil: mint essential oil 3: 3: 2: 2;
preferably, the compound essential oil is oregano essential oil: cinnamon essential oil: clove essential oil: citronella essential oil 3: 3: 2: 2;
preferably, the compound essential oil is oregano essential oil: cinnamon essential oil: clove essential oil: mint essential oil 3: 3: 2: 2;
preferably, the compound essential oil is oregano essential oil: cinnamon essential oil: citronella essential oil: mint essential oil 3: 3: 2: 2;
further, diluting the traditional Chinese medicine air disinfectant to 1-10 mu L/mL by using sterile water;
preferably, the traditional Chinese medicine air disinfectant is diluted to 2 mu L/mL by using sterile water;
preferably, the traditional Chinese medicine air disinfectant is diluted to 3 mu L/mL by using sterile water;
preferably, the traditional Chinese medicine air disinfectant is diluted to 5 mu L/mL by using sterile water;
compared with the prior art, the invention has the following beneficial effects: compared with the analysis and the comparison of the disinfection treatment effects of the SANSEI air disinfectant, the air disinfectant disclosed by the invention can effectively kill harmful microorganisms in the air under the condition that the total number of bacterial colonies before air disinfection is not obviously different, so that the disinfected indoor air meets the quality standard of the total number of bacterial colonies of air in a type II air environment pollution area specified by the state of Ministry of health. Compared with the SANSEI household air sterilization disinfectant, the air sterilization disinfectant has obviously better sterilization effect than the SANSEI air sterilization disinfectant, and has definite air sterilization effect. The sterilizing rate of the traditional Chinese medicine for on-site sterilization and killing of the air disinfectant is about 72.41-89.12%, pathogenic bacteria in the air can be effectively removed, no toxic or side effect is caused to people, the air disinfectant is safe and reliable to use under the condition of someone, no adverse effect is caused to indoor air, a faint scent traditional Chinese medicine smell can be emitted during disinfection, the peculiar smell of the indoor air can be removed, and people feel happy. When the device is used in various occasions, indoor personnel can be protected without leaving and adding any shielding.
In conclusion, the air disinfectant disclosed by the invention is a safe, efficient, environment-friendly, economic and convenient indoor air freshening disinfectant, is suitable for indoor air freshening disinfection under the condition that people smoke in indoor public places, can effectively humidify indoor air and freshen the indoor air when the indoor air is freshened and disinfected, and is particularly suitable for indoor air freshening disinfection in public areas with low air temperature, dry air and tight doors and windows in winter.
Drawings
FIG. 1 is a line graph of OD values of Escherichia coli of different concentrations of 6 single essential oils;
FIG. 2 is a line graph showing OD value curves of different concentrations of 6 single essential oils against Candida albicans;
FIG. 3 is a line graph of OD values of 6 single essential oils with different concentrations against Staphylococcus aureus;
FIG. 4 is a representative graph of the bacteriostatic effect of various essential oils on Escherichia coli; in the figure: 1. compound essential oil 1; 2. compound essential oil 2; 3. compound essential oil 3; 4. compound essential oil 4; 5. compound essential oil 5; 6. compound essential oil 6;
FIG. 5 is a graph showing the bacteriostatic effect of various essential oils on Candida albicans; in the figure: 1. compound essential oil 1; 2. compound essential oil 2; 3. compound essential oil 3; 4. compound essential oil 4; 5. compound essential oil 5; 6. compound essential oil 6;
FIG. 6 is a representative graph of the bacteriostatic effect of various essential oils on Staphylococcus aureus; in the figure: 1. compound essential oil 1; 2. compound essential oil 2; 3. compound essential oil 3; 4. compound essential oil 4; 5. compound essential oil 5; 6. compound essential oil 6;
FIG. 7 is a representative graph of the bactericidal effect of the compound essential oil; in the figure: A. blank; B. 1 mu L/mL compound essential oil 1; C. 2 mu L/mL compound essential oil 1; D. 1 mu L/mL compound essential oil 2; E. 2 mu L/mL compound essential oil 2; F. 1 mu L/mL compound essential oil 3; G. 2 mu L/mL compound essential oil 3;
FIG. 8 is a representative diagram of the killing effect on site; in the figure: a1, 1 mu L/mL compound essential oil 2 before disinfection; a2, and disinfecting with 1 mu L/mL compound essential oil 2; b1, before sterilization of a positive control group (SANSEI air disinfectant); b2, sterilizing a positive control group (SANSEI air disinfectant); c1, before negative control (20% ethanol solution) sterilization; c2, negative control (20% ethanol solution) after sterilization.
Detailed Description
The following will explain a Chinese medicinal air disinfectant in detail with reference to specific embodiments.
A traditional Chinese medicine air disinfectant comprises the following components in percentage by volume: the compound essential oil comprises 10% of compound essential oil and 90% of solvent, wherein the compound essential oil is oregano essential oil: cinnamon essential oil: litsea cubeba essential oil: clove essential oil 3: 3: 2: 2. origanum essential oil: cinnamon essential oil: litsea cubeba essential oil: citronella essential oil 3: 3: 2: 2. origanum essential oil: cinnamon essential oil: litsea cubeba essential oil: mint essential oil 3: 3: 2: 2. origanum essential oil: cinnamon essential oil: clove essential oil: citronella essential oil 3: 3: 2: 2. origanum essential oil: cinnamon essential oil: clove essential oil: mint essential oil 3: 3: 2: 2 or oregano essential oil: cinnamon essential oil: citronella essential oil: mint essential oil 3: 3: 2: 2, the solvent is dimethyl sulfoxide; diluting the traditional Chinese medicine air disinfectant to 1, 2, 3, 5 and 10 mu L/mL by using sterile water. The invention utilizes the oregano essential oil to prepare the pure natural air disinfectant with the bactericidal effect, and can effectively kill microorganisms in the air while removing peculiar smell, refreshing and increasing aroma, thereby improving the air quality.
The sterilization effect of the traditional Chinese medicine air disinfectant is preliminarily detected and compared with the sterilization effect of the air disinfectant in the prior art.
1 Experimental materials and instruments
1.1 Experimental materials
MH agar medium (hangzhou microbial agents ltd); MH broth medium (hangzhou microbial agents ltd); staphylococcus aureus (ATCC29213), Candida albicans (ATCC SC5314), Escherichia coli (ATCC25923), all provided by the university of traditional Chinese medicine and etiology biology and immunology research and study laboratory in Jiangxi. Litsea cubeba essential oil, clove essential oil, citronella essential oil, mint essential oil, cinnamon essential oil, oregano essential oil, absolute ethyl alcohol (Xiong science corporation), cefminox sodium for injection (Nanchang Lijian pharmaceutical Co., Ltd.), 0.1M PBS buffer solution (Beijing Solepupo science and technology Co., Ltd.), SANSEI air disinfectant (Nicoti san Biotech Co., Ltd.), dimethyl sulfoxide (DMSO, Fochen chemical reagent Co., Ltd.)
1.2 laboratory instruments and apparatus
HFsafeI1200LC biosafety cabinet (shanghai science instruments ltd); GSP-9080MBE water-proof constant temperature incubator (Shanghai Boxun industries, Ltd.); GZX-9030MBE electric heating air blast drying oven (Shanghai Boxun practice Co., Ltd.) medical equipment factory; SQP electronic balance (sydows scientific instruments (beijing) ltd); thermo multifunctional enzyme-linked immunosorbent assay (Chongqing Yong Aile laboratory instruments Co., Ltd.); THZ-92C gas bath constant temperature oscillator (Shanghai Boxun industries, Inc. medical facilities); Smart-Q15 UT laboratory pure water system (Shanghai and Tai instruments Co., Ltd.); P70D20TL-D4 microwave oven (Glanshi microwave Electrical appliances Co., Ltd.); a vertical pressure steam sterilizer (Shanghai Boxun practice Co., Ltd.); YCD-EL200 refrigerator (Mike Meiling)
All containers, handling tools and reagents used in the experimental process must be sterilized in an autoclave (103.4kPa, 121 ℃, 30min) before use to ensure their sterility and prevent contamination by infectious microbes.
2 method of experiment
2.1 preparation of bacterial suspension
Recovering Candida albicans, Staphylococcus aureus and Escherichia coli, separating, purifying, inoculating into broth culture medium, culturing for 24 hr, and configuring to have bacteria number of about 108 McLeod turbidity (CFU. mL)-1) And then standby.
2.2 evaluation of the bacteriostatic Effect
2.2.1 determination of minimum inhibitory concentration
The method adopts a trace broth two-fold dilution method. According to the titer or content of the essential oil and the medicament and the required volume, the essential oil is diluted to the required concentration by DMSO, and the cefminox sodium for injection is diluted to 5 percent by using sterile water. The initial concentration of the origanum and cinnamon essential oil is 400 mu L/mL, the two-fold decreasing concentration dilution is sequentially carried out on No. 2-10 holes in a 96-well plate, a culture medium and a bacterial liquid are added into No. 11 holes to be used as positive control, the culture medium and the essential oil are added into an adjacent row to be used as negative control, and DMSO and 5% cefamandole sodium for injection are added into F, G rows to be used as negative and positive drug control in the same method. The initial concentration of other essential oil is 40 mu L/mL, the dilution with twice decreasing concentration is carried out in the holes B to F in a 96-well plate in sequence, the culture medium and the bacterial liquid are added into the hole G to be used as a positive control, the culture medium and the essential oil are added into the adjacent row to be used as a negative control, and DMSO and 5 percent cefminox sodium for injection are added into the 10 and 11 rows to be used as a negative and positive drug control in the same method. After the preparation, the culture plate is placed in a constant temperature incubator at 37 ℃ for 24 hours, and the result is observed. And (4) visually observing under a black background, wherein the solution of the positive control hole is clear and bright, the bacteria of the negative control hole grow well, and the concentration of the drug in the lowest concentration hole with clear and bright solution is the MIC. Then, the OD value of the solution in the culture well was measured by a microplate reader under 450 nm. The experiments were repeated three times and averaged. (results are shown in Table 2)
2.2.2 determination of minimum Bactericidal concentration
After MIC is determined, 10. mu.L of culture in a clear sterile growth well is taken by a pipette, evenly spread on MH agar plates, placed in a constant temperature incubator at 37 ℃ for 24h, and checked by a viable count method to obtain the minimum drug concentration of which the average is less than 5, namely the MBC of the drug. (results are shown in Table 3)
2.2.3 agar perforation method
Compounding essential oil according to the formula of table 1
Table 1 compound essential oil formula table
Preparing sterile nutrient agar culture medium, adding 100 μ L indicator bacteria stock solution, coating, making into agar plate with bacteria, and punching with hole puncher to obtain plate with uniform size and diameter of 5mmAdding 100 μ L of single component essential oil into 5 holes respectivelyDMSO is 10% compound essential oil of solventDMSO and 0.5% cefamandole, culturing the culture medium in a 37 ℃ incubator for 24 hours, and observing the growth state of bacteria. (results are shown in Table 4)
2.3 evaluation of the disinfecting Effect of air disinfectants
2.3.1 tube culture plate counting method
Experimental groups:diluting the air disinfectant with sterile water to 10, 5, 3, 2, 1 μ L/mL1mL of each air disinfectant with each concentration is taken in a test tube, 1mL of escherichia coli is added for action for 10 minutes, and 8mL of sterile PBS buffer solution is added respectively.
Control group: 1mL of sterile water was put into a test tube, 1mL of E.coli was added thereto, the reaction was carried out for 10 minutes, and 8mL of sterile PBS buffer was added thereto. Each 100. mu.L of the culture medium was inoculated to a nutrient agar plate, the medium was incubated at 37 ℃ for 24 hours, the results were observed, the number of colonies was recorded, and the sterilization rate was calculated. (results are shown in Table 5)
2.3.2 evaluation of the on-site Fungicide Effect of air disinfectants
Dissolving the compound essential oil 2 in absolute ethyl alcohol, and then diluting with sterile water to ensure that the volume fractions of the compound essential oil 2 and the ethyl alcohol in the mixed solution are respectively 0.1% and 20%.
SANSEI disinfectant is used as a positive control, and 20% alcohol solution is used as a negative control. Randomly selecting 3 classrooms, ventilating with normal air, uniformly spraying the medicine into the whole space for disinfection, using about 30mL each time, timing after spraying, and closing the door and window for fumigation for 30 min.
The sampling method comprises the following steps: sampling air before and after spraying, sampling by a standard five-point method, respectively placing sterile LB culture medium with the diameter of 9cm at four corners and the center of a room, wherein the distance from the room to the ground is about 1m, sampling for 1 time respectively before and after disinfection for 30min, culturing in a 37 ℃ incubator, observing the result after 24h, recording the number of bacterial colonies, and calculating the sterilization rate. (results are shown in Table 6)
The total number of colonies in the air was 50000N/AT (cfu/m)3) Wherein A is the area of the plate (cm)2) T is the plate exposure time (min) and N is the plate average colony count (cfu/plate).
The bactericidal ratio (number of bacteria before disinfection-number of bacteria after disinfection)/number of bacteria before disinfection × 100%
And (4) judging the standard: the total number of colonies in the air is less than or equal to 10cfu/m3Meets the I-type environmental standard; the total number of colonies in the air is less than or equal to 200cfu/m3Meets the II-class environmental standard; the total number of colonies in the air is less than or equal to 500cfu/m3Meets the III-class environmental standard.
The statistical method comprises the following steps: data were statistically analyzed using SPSS 11.5. The experimental data are expressed as mean ± standard deviation (x ± s). Significant differences among groups were analyzed by analysis of variance (ANOVA), and if significant, further two-by-two comparisons were made with Student-Neuman-Keuls test (SNK), P <0.05 indicates statistical differences
2.4 odor and appearance evaluation
And (4) carrying out comprehensive judgment after evaluating appearance indexes such as color, turbidity, layering and the like and odor by multiple persons.
3 results and analysis of the experiments
3.196 Orifice plate method for detecting Minimum Inhibitory Concentration (MIC) and minimum bactericidal concentration result (MBC) of 6 essential oils
3.1.1 MIC results
Observing the 96-well plate cultured for 24h to see whether the solution is clear and bright, and analyzing by turbidimetry to obtain the minimum inhibitory concentration of various essential oils, the results are shown in Table 2
TABLE 26 minimum inhibitory concentrations (μ L/mL) of essential oils against 3 indicator bacteria
3.1.2MBC results (μ L/mL)
TABLE 36 minimum germicidal concentrations of essential oils to 3 indicator bacteria (μ L/mL)
As can be seen from tables 2 and 3, the cinnamon and oregano essential oils have the best bacteriostatic effect on 3 indicator bacteria, and the litsea cubeba essential oil and the mint essential oil have the worst bacteriostatic effect.
3.2 agar perforation results
TABLE 4 evaluation of the bacteriostatic effects of 6 single essential oils and compound essential oils on 3 indicator bacteria by agar perforation method
Note: the results are the average of the results of 3 experiments
From table 4, it can be seen that the compound essential oils 1, 2, and 3 have good bacteriostatic effect.
3.3 results of tube culture plate counting method
Table 53 sterilizing effect of different concentrations of compound essential oil
Note: the results are the average of the results of 3 experiments
As can be seen from Table 5, the 3 kinds of compound essential oil have good bactericidal effect, but the compound essential oil 2 with 1 muL/mL has pleasant smell, and the liquid does not delaminate after being placed,therefore, 1 mu L/mL of compound essential oil 2 is selected as the formula of the traditional Chinese medicine air disinfectant。
3.4 on-site killing effect
TABLE 6 natural air sterilizer effect (х + -s, n ═ 5)
Note: compared with the pre-disinfection solution, the difference of P # less than 0.05 is obvious; compared with SANSEI air disinfectant, the difference is obvious when P is less than 0.05; the difference of P x < 0.01 is very significant compared with 20% ethanol solution
As can be seen from Table 6, the air disinfectant of the present application has a significantly superior bactericidal effect to SANSEI air disinfectant purchased on the market.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. The traditional Chinese medicine air disinfectant is characterized by comprising the following components in percentage by volume: the compound essential oil comprises 10% of compound essential oil and 90% of solvent, wherein the compound essential oil is oregano essential oil: cinnamon essential oil: litsea cubeba essential oil: citronella essential oil 3: 3: 2: 2, the solvent is dimethyl sulfoxide.
2. The traditional Chinese medicine air disinfectant as claimed in claim 1, wherein the compound essential oil is oregano essential oil: cinnamon essential oil: litsea cubeba essential oil: clove essential oil 3: 3: 2: 2.
3. the traditional Chinese medicine air disinfectant as claimed in claim 1, wherein the compound essential oil is oregano essential oil: cinnamon essential oil: litsea cubeba essential oil: mint essential oil 3: 3: 2: 2.
4. the traditional Chinese medicine air disinfectant as claimed in claim 1, wherein the compound essential oil is oregano essential oil: cinnamon essential oil: clove essential oil: citronella essential oil 3: 3: 2: 2.
5. the traditional Chinese medicine air disinfectant as claimed in claim 1, wherein the compound essential oil is oregano essential oil: cinnamon essential oil: clove essential oil: mint essential oil 3: 3: 2: 2.
6. the traditional Chinese medicine air disinfectant as claimed in claim 1, wherein the compound essential oil is oregano essential oil: cinnamon essential oil: citronella essential oil: mint essential oil 3: 3: 2: 2.
7. the traditional Chinese medicine air disinfectant as claimed in any one of claims 1 to 6, wherein the traditional Chinese medicine air disinfectant is diluted to 1 to 10 μ L/mL with sterile water.
8. The traditional Chinese medicine air disinfectant of claim 7, wherein the traditional Chinese medicine air disinfectant is diluted to 2 μ L/mL with sterile water.
9. The traditional Chinese medicine air disinfectant of claim 7, wherein the traditional Chinese medicine air disinfectant is diluted to 3 μ L/mL with sterile water.
10. The traditional Chinese medicine air disinfectant of claim 7, wherein the traditional Chinese medicine air disinfectant is diluted to 5 μ L/mL with sterile water.
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