CN103947686A - Traditional Chinese medicine air disinfectant, preparation method and application of traditional Chinese medicine air disinfectant - Google Patents
Traditional Chinese medicine air disinfectant, preparation method and application of traditional Chinese medicine air disinfectant Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine air disinfectant, a preparation method and application of the traditional Chinese medicine air disinfectant. The traditional Chinese medicine air disinfectant consists of the following components by volume percent: 1-3% of traditional Chinese medicine extract, and 97-99% of a solvent, wherein the traditional Chinese medicine extract is cinnamon essential oil, and the solvent is water. The traditional Chinese medicine air disinfectant adopts the low-content cinnamon essential oil which is mixed with water, so that the bactericidal effect of a traditional Chinese medicine compound can be maintained, meanwhile the irritation to skin of a human body can be alleviated, and also the safety of the compound in use can be improved. The traditional Chinese medicine air disinfectant is pure and fresh in smell, and is convenient to use.
Description
Technical field
The invention belongs to Chinese medical extract application, relate to a kind of Chinese medical drug air sanitizing agent and its preparation method and application.
Background technology
Air is one of important channel of pathogenic microorganisms propagation, air sterillization is prevention and controls air borne disease, preventing the important measures of cross-infection, is the effective tool that prevents that public place from propagating as pathogenic microorganisms in hospital, entry and exit port, public transport.The incidence of disease of air borne disease is high in recent years, SARS as 2003, the H5N1 of 2004, the Influenza A H1N1 of outburst in 2009 and outburst serious harm China resident's of H7N9 bird flu in 2013 is healthy, impels people more and more to pay close attention to the problem of indoor microbial contamination.China has a large population, and density is large, and the air sanitizer that how to develop high-efficiency low-toxicity is a urgent problem.
Conventional air disinfection method is chemical method and Physical at present, and chemicals has Peracetic acid, lactic acid, hydrogen peroxide, formaldehyde, 84 thimerosals, glutaraldehyde etc., and physical method has ultraviolet ray irradiation, ozone generator etc.These methods can be killed pathogenic microorganisms fast, valid density is low, but also there are many shortcomings, as poor in toxic, excitant, metal protection, lasting effect, manyly itself form again pollutant, affect to some extent health, and when sterilization all personnel must leave the theatre, operating system will have safeguard procedures; After sterilization, peculiar smell can not be eliminated very soon, and long-term use meeting has the danger of accumulate poisoning etc. to human body.If 84 thimerosal air concentrations are when higher, the mucous membrane of human body, skin are had to stronger stimulation or damage.Lactic acid sterilizing rate is 70%, irritant smell, and corrosivity is strong, and respiratory tract is had to stimulation, and furniture, electrical equipment, metal are had to infringement.Peracetic acid is irritant to mucous membrane, and metal is corrosive.To rat, particularly male mouse liver, renal function and lung, testis can be detrimental to evil to hydrogen peroxide long term.Formaldehyde fumigation hot taste is too dense, has carcinogenicity.The ozone overlong time that ozone generator discharges can make skin produce erythema and ozone poisoning purification, alopecia, fall the side effects such as eyebrow, it is not long that ultraviolet radiation disinfection effect maintains, stop irradiating rear bacterial population and go up very soon, and be subject to fluorescent tube intensity, cleanliness factor, ambient temperature and humidity, shelter and distance affects.At present, various countries are eliminating the stronger air disinfection method of toxic and side effect gradually, adopt the methods such as air cleaner sterilization, laminar flow to purify air, but this method investment is large, and many public places are difficult to carry out.
Chinese herbal medicine is the precious legacy of China, and many herbs in vitros have stronger antibacterial, antivirus action, and has advantages of that excitant is little, toxicity is low, is more and more subject to researcher's attention and the favor in market.Conventional Chinese medical drug air sanitizing method has Chinese medicine fumigation (as rhizoma atractylodis, tarragon), herb liquid spray-on process, Chinese medical drug air sanitizing sheet and Traditional Chinese medicinal aerosol method at present.Wherein easily burning is insufficient for Chinese medicine fumigation, and the smoke particles of generation is floating contaminated air in air, and respiratory mucosa is had to certain stimulation; Traditional Chinese medicinal aerosol is easy to use, and the droplet of ejection is small light thin, is evenly distributed, be beneficial to diffusion, but container cost is higher, prepares more complicated, particularly to can cause air secondary to pollute to make propellant as multiple volatile organic matters such as propane, butane, dimethyl ether, only be suitable for using among a small circle; Herb liquid spray-on process not only has disinfective action, can also make dust sedimentation, and the multi-effects such as humidifying, fresh air, more and more receive publicity.
Chinese medical spray type air sanitizer receives much concern in recent years, and the aspects such as its prescription, formulation have all been obtained to certain progress, has developed the pretty good single of many Disinfection Effects and compound disinfectant.But still have many weak points.Owing to being subject to the impact of Chinese herb quality, extraction process etc., Chinese medical drug air sanitizing agent effect is stable not, and the control of its quality is still lacked to research.Some expensive raw material price, extraction process is complicated, has limited it and has used, have because the bad availability of atomizing effect is not high, result of use is only close with ultraviolet method, although the effect having is better, liquid color is darker, easily pollutes wall, clothing etc.
Therefore, actively find and new be applicable to Chinese medicine composition as Chinese medicine antiseptic, develop there is natural faint scent, safety and environmental protection, bactericidal effect be outstanding, the duration, long new disinfectant had very important clinical meaning and social benefit.In prior art, there is the cinnamon essential oil that adopts 5 ~ 30%, the antibacterial air freshener (CN201310239366.X) being mixed with 70 ~ 95% ethanol.But this freshener is owing to having adopted the ethanol of high concentration, inflammable, and safety in utilization is poor, in addition, the concentration of cinnamon essential oil is high, larger to the excitant of human body.
Summary of the invention
Goal of the invention of the present invention is to provide a kind of Chinese medicine composition, said composition is by cinnamon essential oil and solvent composition, this Chinese medicine composition has natural faint scent, can better kill Escherichia coli, staphylococcus aureus, staphylococcus albus, Candida albicans, natural bacteria is fallen to having good killing action, safety non-toxic, easy to use.
Another object of the present invention is to provide the preparation method of described Chinese medicine composition.
Another object of the present invention is to provide the application of described Chinese medicine composition.
Above-mentioned purpose of the present invention is achieved by following technical solution:
A Chinese medicine composition, is comprised of the following by volume component of percentage calculation: Chinese medical extract 1 ~ 3%, and solvent 97 ~ 99%, wherein, described Chinese medical extract is cinnamon essential oil, described solvent is water.
In the application, adopt the cinnamon essential oil of low content to mix with water, can maintain the bactericidal effect of Chinese medicine composition, reduce its excitant to human body skin simultaneously, the safety while also improving composition use, smell is pure and fresh, is more convenient for using.
Preferably, described Chinese medicine composition is comprised of the following by volume component of percentage calculation: Chinese medical extract 1%, water 99%.
Preferably, described Chinese medicine composition is comprised of the following by volume component of percentage calculation: Chinese medical extract 3%, water 97%.
Preferably, described water is preferably distilled water.
As a kind of preferred version, described Chinese medicine composition is by cinnamon essential oil 1ml, and distilled water 99ml forms.
As a kind of preferred version, described Chinese medicine composition is by cinnamon essential oil 3ml, and distilled water 97ml forms.
The preparation method of described Chinese medicine composition is for to mix cinnamon essential oil and get final product with water.
Cinnamon essential oil or the commercially available cinnamon essential oil of described cinnamon essential oil for adopting conventional method to extract.
The application of described Chinese medicine composition in preparing air sanitizer.
Application in the air sanitizer that described Chinese medicine composition is used with Sprayable in preparation.
Described Chinese medicine composition, when as air sanitizer, can be prepared into spray, or directly sprays and use, or coordinates ultrasonic atomizatio to use with humidifier.
Chinese medicine composition of the present invention, discharges in air with Sprayable as air sanitizer, can effectively kill airborne Escherichia coli, staphylococcus aureus, staphylococcus albus, Candida albicans.
Compared with prior art, the present invention has following beneficial effect:
Cinnamon essential oil has very strong bactericidal action to microorganism, also there is the multiple effects such as antitumor, antiviral, antiulcer, antipyretic, analgesia spasmolysis and desinsection, there is special aromatic odor simultaneously, yet it is more used for the industries such as food and commodity and is used as preservative or spices, the present invention is ground-breaking forms air sanitizer by cinnamon essential oil and water under certain proportioning, with the form of spraying, send forth in air and can kill air microbe, there is outstanding bactericidal effect, simultaneously little to human body excitant, energy disease-prevention health, natural faint scent, safety and environmental protection; Invention is also found, the content of cinnamon essential oil in composition is controlled to 1 ~ 3%, can maintain good bactericidal effect in the long period.
Embodiment
Below in conjunction with specific embodiment, the present invention is further explained, but embodiments of the present invention is not limited in any way.Unless stated otherwise, in embodiment, related reagent, method is the conventional reagent in this area and method.
Embodiment 1 air sanitizer preparation and bactericidal effect experiment thereof
1. disinfectant preparation: 1ml cinnamon essential oil is added in 99ml distilled water, shake up, obtain 100ml disinfectant.This disinfectant is without pressurization, and directly packing in sprayer can be to the room air sterilization that carries out disinfection, simple to operate, applied widely.
2. the bactericidal effect of disinfectant experiment
Suspension quantitative disinfecting test with reference to version < < disinfection technology standard > > regulation in 2008, the bactericidal effect of the disinfectant of detection above-mentioned steps 1 preparation to Escherichia coli (8099), staphylococcus aureus (ATCC 6538), staphylococcus albus (ATCC 8799) Candida albicans (ATCC 10231), concrete detection method and result are as follows.
1) detection method: with reference to the suspension quantitative disinfecting test of version < < disinfection technology standard > > regulation in 2008
Get Escherichia coli (8099), staphylococcus aureus (ATCC 6538), the fresh slant culture of staphylococcus albus (ATCC 8799) Candida albicans (ATCC 10231) 24h, use respectively tryptone physiological saline (TPS) to wash down, after mixing, being diluted to bacteria containing amount is 1 * 10
8cfu/ml～5 * 10
8the bacteria suspension of cfu/ml, standby.
In sterile test tube, add 0.5ml bacteria suspension and 0.3% bSA 0.5ml, mix, put in 20 ℃ of water-baths after 5min, add the 1% cinnamon essential oil aqueous solution (positive control is TPS) 4ml, mix immediately.Act on to the scheduled time, draw respectively 0.5ml bacterium medicine mixed liquor and be added in 4.5ml neutralizer test tube, mix after effect 10min, get this liquid 1.0ml inoculated and cultured, cultivate count plate after 48 hours for 37 ℃.Test repeats 3 times, calculate the viable bacteria concentration (cfu/mL) of each group, and be scaled logarithm value (N), be then calculated as follows and kill logarithm value: logarithm value (No)-experimental group viable bacteria concentration logarithm value (Nx) of killing the average viable bacteria concentration of logarithm value (KL)=control group.Each time kill logarithm value all >=5.00, be judged to be sterilization qualified.
2) result of the test
It is 5min that the 1% cinnamon essential oil aqueous solution is killed the time to Escherichia coli, staphylococcus aureus, staphylococcus albus the shortest, and it is 10min that Candida albicans the shortest killed to the time.
Table 1 cinnamon essential oil aqueous solution bactericidal effect
3. the air sterillization of disinfectant experiment
With reference to the GB/T18204.1-2000 < < public place air microbe method of inspection-total number of bacteria, measure the air sterillization test of > > and version < < disinfection technology standard > > regulation in 2008, the Disinfection Effect of the disinfectant of detection above-mentioned steps 1 preparation to room air.Concrete detection method and result are as follows.
1) detection method: measure the air sterillization test of > > and version < < disinfection technology standard > > regulation in 2008 with reference to the GB/T18204.1-2000 < < public place air microbe method of inspection-total number of bacteria.
The setting of sampled point: select 40m
2room, 5 sampled points are set, indoor corner diagonal intersection point is a sampled point, the mid point of this intersection point and four corner lines is other 4 sampled points.Sampled point is apart from 1 meter, wall, and 1.5 meters of height of sampling, avoid circulations of air place such as air-conditioning, door and window.
Before sterilization, the plain agar flat board of diameter 9cm is placed in to sample point, opens plate lid and expose after 5min, cover ware lid, upset is dull and stereotyped, by 48h in flat board to 37 ℃ incubator, carries out colony counting.
The 1% cinnamon essential oil aqueous solution is according to 10ml/m
3consumption, carry out indoor spraying disinfection.Disinfective action to the scheduled time (30min, 60min, 180min) is placed in sample point by the plain agar flat board of diameter 9cm, opens plate lid and exposes after 5min, covers ware lid, and upset is dull and stereotyped, by 48h in flat board to 37 ℃ incubator, carries out colony counting.Colony counting formula is: bacterial population (cfu/m
3)=50000N/AT(N is clump count, and A is platen area, and A is open-assembly time).
Test repeat 3 times or more than, calculate each extinction rate.Each natural bacteria extinction rate is all >=90% qualified.
。
2) experimental result
The 1% cinnamon essential oil aqueous solution is to disinfection of indoor air after 1 hour, and the extinction rate of natural bacteria is 93.2%, reaches predetermined Disinfection Effect.
Table 2 cinnamon essential oil aqueous solution air sterilization effect
| Time | Bacterial population (cfu/m 3） | Extinction rate (%) |
| 0 | 590 | ? |
| 30min | 105 | 82.2 |
| 60min | 40 | 93.2 |
| 180min | 52 | 91.2 |
Embodiment 2 air sanitizer preparations and bactericidal effect experiment thereof
1. disinfectant preparation: 3ml cinnamon essential oil is added in 97ml distilled water, shake up, obtain 100ml disinfectant.This disinfectant is without pressurization, and directly packing in sprayer can be to the room air sterilization that carries out disinfection, simple to operate, applied widely.
2. the bactericidal effect of disinfectant experiment
Suspension quantitative disinfecting test with reference to version < < disinfection technology standard > > regulation in 2008, the bactericidal effect of the disinfectant of detection above-mentioned steps 1 preparation to Escherichia coli (8099), staphylococcus aureus (ATCC 6538), staphylococcus albus (ATCC 8799) Candida albicans (ATCC 10231), concrete detection method and result are as follows.
1) detection method: with reference to the suspension quantitative disinfecting test of version < < disinfection technology standard > > regulation in 2008
Get Escherichia coli (8099), staphylococcus aureus (ATCC 6538), the fresh slant culture of staphylococcus albus (ATCC 8799) Candida albicans (ATCC 10231) 24h, use respectively tryptone physiological saline (TPS) to wash down, after mixing, being diluted to bacteria containing amount is 1 * 10
8cfu/ml～5 * 10
8the bacteria suspension of cfu/ml, standby.
In sterile test tube, add 0.5ml bacteria suspension and 0.3% bSA 0.5ml, mix, put in 20 ℃ of water-baths after 5min, add the 3% cinnamon essential oil aqueous solution (positive control is TPS) 4ml, mix immediately.Act on to the scheduled time, draw respectively 0.5ml bacterium medicine mixed liquor and be added in 4.5ml neutralizer test tube, mix after effect 10min, get this liquid 1.0ml inoculated and cultured, cultivate count plate after 48 hours for 37 ℃.Test repeats 3 times, calculate the viable bacteria concentration (cfu/mL) of each group, and be scaled logarithm value (N), be then calculated as follows and kill logarithm value: logarithm value (No)-experimental group viable bacteria concentration logarithm value (Nx) of killing the average viable bacteria concentration of logarithm value (KL)=control group.Each time kill logarithm value all >=5.00, be judged to be sterilization qualified.
2) result of the test
The 3% cinnamon essential oil aqueous solution is killed the time to Escherichia coli, staphylococcus aureus, staphylococcus albus and Candida albicans the shortest and is 1min.
Table 3 cinnamon essential oil aqueous solution bactericidal effect
3. the air sterillization of disinfectant experiment
With reference to the GB/T18204.1-2000 < < public place air microbe method of inspection-total number of bacteria, measure the air sterillization test of > > and version < < disinfection technology standard > > regulation in 2008, the Disinfection Effect of the disinfectant of detection above-mentioned steps 1 preparation to room air.Concrete detection method and result are as follows.
1) detection method: measure the air sterillization test of > > and version < < disinfection technology standard > > regulation in 2008 with reference to the GB/T18204.1-2000 < < public place air microbe method of inspection-total number of bacteria.
The setting of sampled point: select 40m
2room, 5 sampled points are set, indoor corner diagonal intersection point is a sampled point, the mid point of this intersection point and four corner lines is other 4 sampled points.Sampled point is apart from 1 meter, wall, and 1.5 meters of height of sampling, avoid circulations of air place such as air-conditioning, door and window.
Before sterilization, the plain agar flat board of diameter 9cm is placed in to sample point, opens plate lid and expose after 5min, cover ware lid, upset is dull and stereotyped, by 48h in flat board to 37 ℃ incubator, carries out colony counting.
The 3% cinnamon essential oil aqueous solution is according to 10ml/m
3consumption, carry out indoor spraying disinfection.Disinfective action to the scheduled time (30min, 60min, 180min) is placed in sample point by the plain agar flat board of diameter 9cm, opens plate lid and exposes after 5min, covers ware lid, and upset is dull and stereotyped, by 48h in flat board to 37 ℃ incubator, carries out colony counting.Colony counting formula is: bacterial population (cfu/m
3)=50000N/AT(N is clump count, and A is platen area, and A is open-assembly time).
Test repeat 3 times or more than.Calculate each extinction rate.Each natural bacteria extinction rate is all >=90% qualified.。
2) experimental result
The 3% cinnamon essential oil aqueous solution to disinfection of indoor air 30min after, the extinction rate of natural bacteria is 97.2%, reaches predetermined Disinfection Effect.
Table 4 cinnamon essential oil aqueous solution air sterilization effect
Comparative example 1 air sanitizer preparation and bactericidal effect experiment thereof
1. disinfectant preparation: 5ml cinnamon essential oil is added in 95ml distilled water, shake up, obtain 100ml disinfectant.
2. the air sterillization of disinfectant experiment
With reference to the GB/T18204.1-2000 < < public place air microbe method of inspection-total number of bacteria, measure the air sterillization test of > > and version < < disinfection technology standard > > regulation in 2008, the Disinfection Effect of the disinfectant of detection above-mentioned steps 1 preparation to room air.Concrete detection method and result are as follows.
1) detection method: measure the air sterillization test of > > and version < < disinfection technology standard > > regulation in 2008 with reference to the GB/T18204.1-2000 < < public place air microbe method of inspection-total number of bacteria.
The setting of sampled point: select 40m
2room, 5 sampled points are set, indoor corner diagonal intersection point is a sampled point, the mid point of this intersection point and four corner lines is other 4 sampled points.Sampled point is apart from 1 meter, wall, and 1.5 meters of height of sampling, avoid circulations of air place such as air-conditioning, door and window.
Before sterilization, the plain agar flat board of diameter 9cm is placed in to sample point, opens plate lid and expose after 5min, cover ware lid, upset is dull and stereotyped, by 48h in flat board to 37 ℃ incubator, carries out colony counting.
The 5% cinnamon essential oil aqueous solution is according to 10ml/m
3consumption, carry out indoor spraying disinfection.Disinfective action to the scheduled time (30min, 60min, 180min) is placed in sample point by the plain agar flat board of diameter 9cm, opens plate lid and exposes after 5min, covers ware lid, and upset is dull and stereotyped, by 48h in flat board to 37 ℃ incubator, carries out colony counting.Colony counting formula is: bacterial population (cfu/m
3)=50000N/AT(N is clump count, and A is platen area, and A is open-assembly time).
Test repeat 3 times or more than.Calculate each extinction rate.Each natural bacteria extinction rate is all >=90% qualified.
。
2) experimental result
The 5% cinnamon essential oil aqueous solution to disinfection of indoor air 30min after, the extinction rate of natural bacteria is 95.9%, reaches predetermined Disinfection Effect.
Table 5 cinnamon essential oil aqueous solution air sterilization effect
It is generally acknowledged, the higher bactericidal property of concentration of cinnamon essential oil is better, but from the result of table 2, table 4 and table 5, can find out, cinnamon essential oil, under 1%, 3%, 5% concentration, all can reach more than 90% extinction rate, but the prolongation along with the time, the composition bacterium extinction rate of 5% concentration obviously declines, and after 180min, has dropped to 70%, while showing the excessive concentration of cinnamon essential oil, airborne lasting antiseptic property is subject to significant limitation.Inventor's deduction, the concentration of cinnamon essential oil is relevant to the droplet size that spraying forms, and when cinnamon essential oil excessive concentration, the drop size of formation is relatively large, and sedimentation more easily occurs, thereby causes its aerial lasting Disinfection Effect not good.Cinnamon essential oil concentration is 1 ~ 3% time, and the drop size of formation is less, more can be scattered in constantly in air, in 180min, still can keep obvious Disinfection Effect.
The foregoing is only preferred embodiment of the present invention, be not limited to the present invention, within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (8)
1. a Chinese medicine composition, is characterized in that, the following by volume component of percentage calculation, consists of: Chinese medical extract 1 ~ 3%, and solvent 97 ~ 99%, wherein, described Chinese medical extract is cinnamon essential oil, described solvent is water.
2. Chinese medicine composition according to claim 1, is characterized in that, consists of: Chinese medical extract 1%, water 99% the following by volume component of percentage calculation.
3. Chinese medicine composition according to claim 1, is characterized in that, consists of: Chinese medical extract 3%, water 97% the following by volume component of percentage calculation.
4. Chinese medicine composition according to claim 1, is characterized in that, described Chinese medicine composition is by cinnamon essential oil 1ml, and distilled water 99ml forms.
5. Chinese medicine composition according to claim 1, is characterized in that, described Chinese medicine composition is by cinnamon essential oil 3ml, and distilled water 97ml forms.
6. the preparation method of Chinese medicine composition described in any one claim in claim 1 to 5, is characterized in that, cinnamon essential oil is mixed and be get final product with water.
7. preparation method according to claim 6, is characterized in that, cinnamon essential oil or the commercially available cinnamon essential oil of described cinnamon essential oil for adopting conventional method to extract.
8. the application of Chinese medicine composition in preparing air sanitizer described in any one claim in claim 1 to 5.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111345321A (en) * | 2020-03-12 | 2020-06-30 | 云南西草资源开发有限公司 | Antibacterial air purifying agent and preparation method thereof |
| CN111494470A (en) * | 2020-05-18 | 2020-08-07 | 刘淼淼 | A Chinese medicinal composition with antibacterial, disinfectant, and mind and body relaxing effects, and its application |
| CN112913858A (en) * | 2021-04-23 | 2021-06-08 | 云南自然谷生物开发有限公司 | Plant disinfectant for public places |
| CN113080206A (en) * | 2021-04-13 | 2021-07-09 | 江西中医药大学 | Aromatic essential oil air disinfectant and preparation method thereof |
| CN113729038A (en) * | 2021-07-13 | 2021-12-03 | 江西省科学院微生物研究所 | Traditional Chinese medicine air disinfectant |
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| EP1146111A1 (en) * | 2000-04-14 | 2001-10-17 | The Procter & Gamble Company | Process of disinfecting a hard-surface with a composition comprising cinnamon oil and/or an active thereof |
| US20090087502A1 (en) * | 2007-09-27 | 2009-04-02 | Simon Jameson | Non-toxic Antimicrobial Composition |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1146111A1 (en) * | 2000-04-14 | 2001-10-17 | The Procter & Gamble Company | Process of disinfecting a hard-surface with a composition comprising cinnamon oil and/or an active thereof |
| US20090087502A1 (en) * | 2007-09-27 | 2009-04-02 | Simon Jameson | Non-toxic Antimicrobial Composition |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111345321A (en) * | 2020-03-12 | 2020-06-30 | 云南西草资源开发有限公司 | Antibacterial air purifying agent and preparation method thereof |
| CN111494470A (en) * | 2020-05-18 | 2020-08-07 | 刘淼淼 | A Chinese medicinal composition with antibacterial, disinfectant, and mind and body relaxing effects, and its application |
| CN113080206A (en) * | 2021-04-13 | 2021-07-09 | 江西中医药大学 | Aromatic essential oil air disinfectant and preparation method thereof |
| CN112913858A (en) * | 2021-04-23 | 2021-06-08 | 云南自然谷生物开发有限公司 | Plant disinfectant for public places |
| CN113729038A (en) * | 2021-07-13 | 2021-12-03 | 江西省科学院微生物研究所 | Traditional Chinese medicine air disinfectant |
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| CN103947686B (en) | 2016-09-14 |
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