CN113170801A - Disinfectant and preparation method and application thereof - Google Patents
Disinfectant and preparation method and application thereof Download PDFInfo
- Publication number
- CN113170801A CN113170801A CN202110454922.XA CN202110454922A CN113170801A CN 113170801 A CN113170801 A CN 113170801A CN 202110454922 A CN202110454922 A CN 202110454922A CN 113170801 A CN113170801 A CN 113170801A
- Authority
- CN
- China
- Prior art keywords
- disinfectant
- stirring
- test
- hopper
- prepared
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Inorganic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a disinfectant, which is mainly prepared from sodium hypochlorite, sodium chloride, a pH regulator, an aloe extract, glycyrrhizic acid and purified water. The disinfectant is prepared in a vacuum emulsifying mixer, and is vacuumized to ensure sealing, light-proof and no air. The disinfectant provided by the invention has the main effective component of hypochlorous acid, has a killing effect on bacteria, mould and viruses, can kill the bacteria, the mould and the viruses within 30s for a log value of more than 5, has no residue after sterilization, is not irritant, and is non-toxic and harmless. The synergistic effect of hypochlorous acid, glycyrrhizic acid and aloe is utilized, so that the sterilizing agent can efficiently sterilize, is safe and nontoxic to use, has no residue and is harmless to human bodies.
Description
Technical Field
The invention belongs to the technical field of disinfectant, and particularly relates to a disinfectant, and a preparation method and application thereof.
Background
With the development of scientific technology and the continuous improvement of the requirements of human beings on living quality, the requirements on food quality are improved, fruits and vegetables are only cleaned by tap water and can only remove dust and impurities on the surfaces, but bacteria on the surfaces cannot be removed, and gastrointestinal discomfort and other symptoms can occur after the disinfectant is used, so that the disinfection of fruits, vegetables and tableware is imperative, the household disinfectants in the current market mainly comprise alcohol, 84 disinfectant and the like, the disinfection methods used for tableware are usually ultraviolet and infrared, the disinfection is carried out by using a disinfection cabinet, the disinfection cabinet occupies the kitchen area, the tableware is easy to age after long use, and the fruits and the vegetables cannot be disinfected, so that the disinfectant for the fruits, the vegetables and the tableware is developed, and the requirements on the living quality of people are met.
The hypochlorous acid disinfectant is an aqueous solution containing stable hypochlorous acid molecules in a stock solution, and is a high-efficiency disinfectant with wide bactericidal spectrum and strong killing capacity. Hypochlorous acid, a neutral inorganic small molecule, can penetrate cell walls and cell membranes of microorganisms, destroy biological substances such as proteins and nucleic acids of the microorganisms, and cause the microorganisms to die. The using effect of the hypochlorous acid disinfectant is influenced by the content of organic matters, the concentration of the used disinfectant, the temperature, the illumination and other factors, so that more organic matters exist in a disinfection object system, and the sterilization effect is relatively reduced; the disinfectant has increased concentration and temperature, and has enhanced bactericidal effect. The disinfectant containing hypochlorous acid is a non-toxic, harmless, non-irritant, mild and efficient disinfectant, and has no residue after use, so that the disinfectant can completely meet the requirements for disinfecting fruits, vegetables and tableware.
The hypochlorous acid disinfectant in the market at present is usually an electrolytic method and a chemical method, wherein the electrolytic method for producing hypochlorous acid consumes a large amount of electric energy, and an expensive noble metal electrode is easy to damage, so that the use cost of production equipment is high. In addition, hypochlorous acid solution generated by the electrolysis method has low pH (generally 2-4) and is difficult to accurately control, so that hypochlorous acid is unstable and is easy to decompose, and chlorine gas is generated. The chemical method is to adjust the pH value to 3-7 by hypochlorite to produce hypochlorous acid, but the same problem exists with the electrolytic method, and the prepared hypochlorous acid is unstable. The prepared hypochlorous acid has a very limited shelf life, so the stability problem of the hypochlorous acid is broken through, the market prospect of the hypochlorous acid as a disinfectant is very wide, and the hypochlorous acid has great significance in the field of disinfection.
Disclosure of Invention
The invention aims to solve the problems and provides a mild and efficient disinfectant which is easy to flush, non-toxic and harmless, has no residue after use.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a disinfectant, which is mainly prepared from sodium hypochlorite, sodium chloride, a pH regulator, an aloe extract, glycyrrhizic acid and purified water.
The feed comprises the following raw materials in parts by weight:
further, the pH regulator is at least one of hydrochloric acid and phosphoric acid of 0.1 mol/L.
Further, the aloe extract is aloe vera, and the concentration of the aloe extract is 1 g/ml. Mixing fresh aloe and water at a mass ratio of 1:8, pulverizing, squeezing, filtering, and concentrating to obtain 1g/mL solution.
Furthermore, the purified water is prepared by drinking water which sequentially passes through a multi-media filter, an activated carbon filter, a softener and a secondary reverse osmosis device.
The invention also provides a preparation method of the disinfectant, which comprises the following steps:
the method comprises the following steps: adding purified water into a vacuum emulsification stirrer, adding sodium chloride from a hopper of the vacuum emulsification stirrer, vacuumizing and stirring;
step two: adding sodium hypochlorite into a vacuum emulsification stirrer from a hopper, vacuumizing and stirring;
step three: slowly adding a pH regulator and glycyrrhizic acid from a hopper, stirring while adding, regulating the pH value to 3.0-6.5, vacuumizing and continuously stirring to react to generate hypochlorous acid;
step four: slowly adding Aloe extract from hopper, and stirring.
Further, in the first step and the second step, the stirring speed of the vacuum emulsification stirrer is 20HZ, and the stirring time is 5 min; in the third step, the stirring speed is 20HZ, and the stirring time is 10 min.
The invention also provides the application of the disinfectant in cleaning vegetables, fruits and tableware.
The using method comprises the following steps: sterilizing vegetables and fruits, soaking or spraying the fruits and vegetables with the product for 30min after washing with clear water, and draining. For tableware disinfection, the tableware is cleaned conventionally to remove oil stains, washed by clean water, soaked in the product for 30min and then drained.
Compared with the prior art, the invention has the following advantages: the disinfectant is prepared in a vacuum emulsifying mixer, and is vacuumized to ensure sealing, light-proof and no air. The product is in a vacuum state, is sealed, is protected from light and has no air, so that the time for contacting air is reduced, the product performance is stable, and the quality guarantee period can reach 2 years; glycyrrhizic acid has effects of removing toxic substance, resisting inflammation, resisting oxidation, and resisting bacteria, and Aloe has natural antibacterial and antiinflammatory effects. The disinfectant provided by the invention has the main effective component of hypochlorous acid, has a killing effect on bacteria, mould and viruses, can kill the bacteria, the mould and the viruses within 30s for a log value of more than 5, has no residue after sterilization, is not irritant, and is non-toxic and harmless. The synergistic effect of hypochlorous acid, glycyrrhizic acid and aloe is utilized, so that the sterilizing agent can efficiently sterilize, is safe and nontoxic to use, has no residue and is harmless to human bodies.
Detailed Description
The present invention is described in further detail for the purpose of better understanding the objects, structure and function of the invention.
Example 1
The disinfectant comprises the following raw materials in parts by weight: 0.01 part of sodium hypochlorite, 0.01 part of sodium chloride, 0.01 part of pH regulator, 0.01 part of aloe extract, 0.01 part of glycyrrhizic acid and 100 parts of purified water.
Wherein the pH regulator is 0.1mol/L hydrochloric acid. The purified water is prepared by drinking water which sequentially passes through a multi-media filter, an activated carbon filter, a softener and a secondary reverse osmosis device.
The preparation method comprises the following steps of according to the parts by weight of the raw materials: adding purified water into a vacuum emulsification stirrer, adding sodium chloride from a hopper of the vacuum emulsification stirrer, vacuumizing and stirring, wherein the stirring speed of the vacuum emulsification stirrer is 20HZ, and stirring for 5 min; step two, adding sodium hypochlorite into a vacuum emulsification stirrer from a hopper, vacuumizing and stirring, wherein the stirring speed of the vacuum emulsification stirrer is 20HZ, and the stirring time is 5 min; slowly adding hydrochloric acid and glycyrrhizic acid from a hopper, stirring while adding, regulating the stirring speed of a vacuum emulsification stirrer to be 20HZ, regulating the pH value to be 3.0 for 10min, vacuumizing, continuously stirring, and reacting to generate hypochlorous acid; step four: slowly adding Aloe extract from hopper, and stirring.
Example 2
The disinfectant comprises the following raw materials in parts by weight: 0.25 part of sodium hypochlorite, 0.5 part of sodium chloride, 0.5 part of pH regulator, 1 part of aloe extract, 0.5 part of glycyrrhizic acid and 100 parts of purified water.
Wherein the pH regulator is 0.1mol/L hydrochloric acid. The purified water is prepared by drinking water which sequentially passes through a multi-media filter, an activated carbon filter, a softener and a secondary reverse osmosis device.
The preparation method comprises the following steps of according to the parts by weight of the raw materials: adding purified water into a vacuum emulsification stirrer, adding sodium chloride from a hopper of the vacuum emulsification stirrer, vacuumizing and stirring, wherein the stirring speed of the vacuum emulsification stirrer is 20HZ, and stirring for 5 min; step two, adding sodium hypochlorite into a vacuum emulsification stirrer from a hopper, vacuumizing and stirring, wherein the stirring speed of the vacuum emulsification stirrer is 20HZ, and the stirring time is 5 min; slowly adding hydrochloric acid and glycyrrhizic acid from a hopper, stirring while adding, regulating the stirring speed of a vacuum emulsification stirrer to be 20HZ, regulating the pH value to be 4.5, vacuumizing, continuously stirring, and reacting to generate hypochlorous acid; step four: slowly adding Aloe extract from hopper, and stirring.
Example 3
The disinfectant comprises the following raw materials in parts by weight: 0.5 part of sodium hypochlorite, 1 part of sodium chloride, 1 part of pH regulator, 2 parts of aloe extract, 1 part of glycyrrhizic acid and 100 parts of purified water.
Wherein the pH regulator is 0.1mol/L phosphoric acid. The purified water is prepared by drinking water which sequentially passes through a multi-media filter, an activated carbon filter, a softener and a secondary reverse osmosis device.
The preparation method comprises the following steps of according to the parts by weight of the raw materials: adding purified water into a vacuum emulsification stirrer, adding sodium chloride from a hopper of the vacuum emulsification stirrer, vacuumizing and stirring, wherein the stirring speed of the vacuum emulsification stirrer is 20HZ, and stirring for 5 min; step two, adding sodium hypochlorite into a vacuum emulsification stirrer from a hopper, vacuumizing and stirring, wherein the stirring speed of the vacuum emulsification stirrer is 20HZ, and the stirring time is 5 min; slowly adding phosphoric acid and glycyrrhizic acid from a hopper, stirring while adding, wherein the stirring speed of the vacuum emulsification stirrer is 20HZ, the stirring time is 10min, the pH value is adjusted to 6.5, and hypochlorous acid is generated by reaction; step four: slowly adding Aloe extract from hopper, and stirring.
Experiment one: available chlorine content
1. Test samples: disinfectant prepared in example 2.
2. Reagent: a sulfuric acid solution with the concentration of 2 mol/L; potassium iodide solution with the concentration of 100 g/L; starch indicator with concentration of 5 g/L.
3. Standard solution: sodium thiosulfate standard solution with the concentration of 0.1000 mol/L.
The experimental method comprises the following steps: the test was repeated 2 times at an ambient temperature of 22.2 ℃ and a relative humidity of 51% according to the determination of the content of available chlorine in 2.2.1.2.1, technical Specification for Disinfection (2002 edition), with the following results:
and (4) conclusion: the disinfectant prepared in example 2 had an available chlorine content of 127 mg/L.
Experiment two: stability test
1. Test samples: disinfectant prepared in example 2.
2. The instrument equipment comprises: biochemical incubator, model LBI-300.
3. Reagent: a sulfuric acid solution with the concentration of 2 mol/L; potassium iodide solution with the concentration of 100 g/L; starch indicator with concentration of 5 g/L.
4. Standard solution: sodium thiosulfate standard solution with the concentration of 0.1000 mol/L.
The experimental method comprises the following steps: the test was repeated 2 times at an ambient temperature of 26.9 ℃ and a relative humidity of 70% according to the determination of the content of available chlorine in sterilization specification 2.2.1.2.1 (2002 edition) and the determination of the stability of the sterilized products 2.2.3, and the following results were obtained:
and (4) conclusion: the disinfectant prepared in example 2 has an available chlorine content of 115.11mg/L after being stored at 37 ℃ for 90 days, and the shelf life of the sample can reach 2 years.
Experiment three: coli killing test
First, equipment
1. Test strains: escherichia coli 8099, the generation of the strain is 4, and bacterial suspension is prepared by using a solution containing 0.03 mol/LPBS.
2. Test samples: disinfectant prepared in example 2.
3. Neutralizing agent: PBS solution containing 0.5% sodium thiosulfate, 0.5% lecithin, 1% Tween 80.
4. Organic interferents: 0.3% bovine serum albumin.
Second, method
1. The inspection basis is as follows: specification for disinfection (2002 edition) 2.1.1.5 neutralizer identification test and 2.1.1.7 quantitative bacteria killing test.
2. Preparation of bacterial suspension: selecting bacteria with a content of 1 × 108CFU/mL～5×108CFU/mL bacterial suspension was subjected to neutralizer identification test.
3. And (3) identification test of a neutralizer: the test groups are: (1) disinfectant and bacterial suspension; (2) (disinfectant + bacterial suspension) + neutralizer; (3) neutralizing agent + bacterial suspension; (4) (disinfectant + neutralizer) + bacterial suspension; (5) diluent + bacterial suspension; (6) diluent + neutralizer + medium. Diluting the sample stock solution by 2 times in the same ratio as the neutralizing agent, and acting for 30 s. The experiment was repeated 3 times.
4. And (3) killing performance test: according to the quantitative sterilization test of the suspension, the action time of the sample stock solution is 15min, 30min and 45min, and the test is repeated for 3 times. The test environment temperature is 22.7 ℃ and the relative humidity is 52%.
Three, result in
1. Neutralizer identification test
3 times of repeated experiments prove that the average number of growing colonies in the 1 st group is aseptic growth, and the average number of growing colonies in the 2 nd group is 3.3 multiplied by 103CFU/mL, similar average number of growing colonies in groups 3, 4, and 5, with an error rate of 4.17% between groups, and sterile growth in group 6.
Negative control was grown aseptically.
2. Killing effect on Escherichia coli
3 times of repeated tests prove that the sample stock solution acts for 15min, and the killing log values of the sample stock solution to the golden escherichia coli are all greater than 5.00.
Negative control was grown aseptically.
Fourth, conclusion
The sample stock solution, namely the disinfectant prepared in example 2, is acted for 30min by using PBS solution containing 0.5% of sodium thiosulfate, 0.5% of lecithin and 1% of Tween 80 as a neutralizing agent, and the killing log values of the disinfectant on escherichia coli are all greater than 5.00.
And (4) testing: simulation on-site sterilization test (food and beverage set)
Firstly, equipment:
1. test strains: escherichia coli 8099, the generation of the strain is 4, and bacterial suspension is prepared by using a solution containing 0.03 mol/LPBS.
2. Test samples: disinfectant prepared in example 2.
3. Neutralizing agent: PBS solution containing 0.5% sodium thiosulfate, 0.5% lecithin, 1% Tween 80.
4. Disinfecting the object: porcelain bowl and porcelain plate.
5. An L-shaped coating rod, a timer, a graduated pipette and the like.
Secondly, the method comprises the following steps:
1. the detection basis is as follows: a simulated on-site identification test of the disinfection effect of 2.1.2.1 disinfectant on food (drink) in disinfection technical Specification (2002 edition).
2. Preparation of bacterial suspension: the bacteria amount should be 1.25 × 107CFU/sample-1.25X 108CFU/sample.
3. Marking a stain area (5.0cm multiplied by 5.0cm) in the middle of a porcelain bowl (dish) by using a sterile specification plate, adding 0.1mL of bacteria, uniformly coating a sterile L rod, and drying for later use; sequentially and regularly placing 30 bacteria-infected samples into a container containing disinfectant solution, completely immersing, removing the disinfectant after acting for a set time, adding 5mL of neutralizer into a bacteria-infected area in a porcelain bowl (dish), and scraping and washing by using an L rod; the reaction time is 10min, 1.0mL of the pour plate is taken and cultured at 37 ℃ for 48h to be used as a test group sample.
3 infected samples are not soaked in the disinfectant, and 5mL of neutralizer is directly added to be used as a positive control sample.
The neutralizing agent, the diluent and the culture medium in the same batch are used as negative control group samples.
3. Test number: 30 blocks. The test environment temperature is 22.7 ℃ and the relative humidity is 52%.
Three, result in
Repeated tests for 30 times show that the sample stock solution has soaking effect for 30min and the killing logarithm value of the sample stock solution to escherichia coli is more than 3.00.
The number of positive control bacteria is 2.8 × 106～5.5×106CFU/mL, average 2.1X 107CFU/sample. Negative control was grown aseptically.
Fourth, conclusion
The results of 30 simulated on-site disinfection tests using PBS containing 0.5% sodium thiosulfate, 0.5% lecithin and 1% tween 80 as neutralizer show that the log liquid of the sample is soaked for 30min, and the log values of the colibacillus killing are all greater than 3.00. Meets the requirements of disinfection technical specification (2002 edition).
And (5) testing: object surface on-site disinfection test (fruit and vegetable)
First, equipment
1. Test sample the disinfectant prepared in example 2.
2. And a neutralizing agent, namely a PBS solution containing 0.5 percent of sodium thiosulfate, 0.5 percent of lecithin and 1 percent of Tween 80.
3. Control sample solution: 0.1% Tween 80 PBS.
4. Sterilizing an object; cucumber.
5. Gauge plate (5cm × 5cm), sterile cotton swab.
6. A timer, a graduated pipette, etc.
Second, method
1. The detection is based on a field identification test of 2.1.2.10 disinfectant on other surfaces in disinfection technical Specification (2002 edition).
2. Before disinfection; the sterile cotton is wiped in a test tube containing 5mL of diluent to be wetted, and a block of the surface of the cleaned and drained cucumber is smeared and sampled, and the sampling is carried out for 8 times in a horizontal-vertical reciprocating mode. After sampling, the sampling end of the cotton swab is cut into the original diluent test tube in a sterile operation mode, and the test tube is rapped 80 times to be used as a positive control group sample.
3. Taking a test sample stock solution, and immersing the cucumbers in the disinfectant for 30 min. After sterilization, the neutralizing agent was used instead of the diluent, and the sterilized block was smeared and sampled in the same manner as the positive control group to be used as a test group sample.
4. Test number: 30 blocks, test ambient temperature 22.7 ℃ and relative humidity 52%. Three, result in
Repeated tests for 30 times show that the sample stock solution has a soaking effect for 30min and a killing logarithm value of >1.00 for natural bacteria on the surface of the cucumber.
Negative control was grown aseptically.
Fourth, conclusion
The test results of 30 times by using PBS solution containing 0.5% of sodium thiosulfate, 0.5% of lecithin and 1% of Tween 80 as a neutralizer show that the sample stock solution has a killing logarithm value of being greater than 1.00 to natural bacteria on the surface of the cucumber after being soaked for 30 min. Meets the requirements of disinfection technical specification (2002 edition).
And (6) test six: acute oral toxicity test (LD)50)
Materials and animals
1. Test sample the disinfectant prepared in example 2.
2. The experimental animals and the feeding environment are 20 SPF-grade KM mice, the male and female parts are half, the weight is 18-22 g, the experimental animals are provided by the Guangdong province medical experimental animal center, the production license number of the experimental animals is SCXK (Guangdong) 2018-002, and the quality qualification number is No. 44007200076138. The feed is provided by the medical experimental animal center of Guangdong province. After the animals are purchased, the animals are raised in an animal room in the barrier environment of the home, and the animals are used after quarantine is qualified, the license number of the experimental animals is used: SYXK (Guangdong) 2018-0137, the temperature of the animal room is 20-26 ℃, and the relative humidity is high; 40 to 70 percent.
Second, method
1. The inspection basis is 2.3.1 of 'Disinfection technical Specification' (2002 edition).
2. Inspection method
2.1 dose design and test grouping, adopting one maximum test, and only setting one dose group of 5000mg/kg body weight. In the experiment, 5.0g of sample is weighed, and pure water is added to 10.0mL and mixed uniformly for testing.
2.2 after fasting overnight without water, the experimental animals are gavaged at 10.0mL/kg body weight, and the samples are taken once, wherein the sample dose is 5000mg/kg body weight. The experimental animals were observed and recorded for intoxication performance, number of deaths and time to death immediately after the administration, observation period 14 d.
2.3 evaluation of results, no death of the test animal within 14 days, and determination of LD50>5000mg/kg body weight. Third, test results
After the infection, the toxic manifestation and death of the mice are not found in the observation period, and the surviving animals are dissected after the observation period is finished, so that no abnormality is seen with naked eyes.
Fourth, conclusion
The acute oral toxicity LD3 of the disinfectant prepared in the test sample, namely the example 2, on female and male KM mice is more than 5000mg/kg of body weight, is of an actual nontoxic grade, and meets the requirements of disinfection technical specification (2002 edition) 2.3.13.1 (1).
Test seven: poliovirus suspension quantitative inactivation assay
First, equipment
1. Test virus strains: poliovirus type 1 (poliovirus-I, PV-I) vaccine strain.
2. Host cell: VERO cells.
3. A disinfectant: the disinfectant prepared in example two.
4. Neutralizer components and concentrations: PBS containing 5g/L sodium thiosulfate.
5. Cell culture bottles and 96-well culture plates.
6. An organic interfering substance; 0.3% bovine serum is derived from protein.
7. Standard hard water (342 mg/L hardness).
8. Cell maintenance medium, cell complete medium and fetal bovine serum.
9. A thermostat, a carbon dioxide incubator, a biological safety cabinet, an adjustable pipettor and sterile equipment.
Second, method
1. The detection basis is as follows: disinfection Specification (2002 edition) items 2.1.1.10.5 and 2.1.1.10.7.
2. Preparation of virus suspension: titer for the assay was 107～108TCID500.1mL of PV-I virus suspension and 0.3% bovine serum free from egg organic interfering substance are subjected to double dilution, and the solution is placed at a constant temperature of 20 ℃ for later use.
3, neutralizer identification test: the disinfectant prepared in the second embodiment is applied for 15min, and the test temperature is constant at 20 ℃. The experiment was repeated 3 times.
4. Virus inactivation test: the disinfectant prepared in the second embodiment is applied for 15min, 30min and 45min, and the test temperature is constant at 20 ℃. The experiment was repeated 3 times.
Three, result in
1. Neutralizer identification test
After 3 repeated tests, under the constant temperature test condition of 20 ℃, the identification result of the disinfectant neutralizer prepared in the second embodiment is as follows: the mean titer value of the group 1 virus was 3.78, the mean titer value of the group 2 virus was 5.0O, the mean titer values of the group 3, 4 and 5 viruses were 7.56, 7.44 and 7.67, respectively, and the group 6 was a negative control group.
2, inactivating effect on poliovirus
After 3 repeated tests, the disinfectant prepared in the second embodiment is applied for 30min under the constant temperature test condition of 20 ℃, and the average inactivation log value of the poliovirus is greater than 4.00.
Negative control cells grew well without cytopathic effects.
Fourth, conclusion
1. After 3 repeated tests, under the condition of a constant temperature test at 20 ℃, the neutralizing agent solution of PBS containing 5g/L of sodium thiosulfate can effectively neutralize the residual effect of the disinfectant prepared in the second embodiment on the poliovirus type I vaccine strain, and the neutralizing agent and a neutralization product basically have no influence on the growth of the poliovirus type I vaccine strain and cells.
2. After 3 repeated tests, the disinfectant stock solution prepared in the second embodiment is applied for 30.0min under the constant temperature test condition of 20 ℃, the average inactivation log value of the poliovirus type I vaccine strain is more than 4.00, and the disinfectant meets the qualified regulation of the disinfection technical Specification (2002 edition).
It is to be understood that the present invention has been described with reference to certain embodiments, and that various changes in the features and embodiments, or equivalent substitutions may be made therein by those skilled in the art without departing from the spirit and scope of the invention. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the invention without departing from the essential scope thereof. Therefore, it is intended that the invention not be limited to the particular embodiment disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.
Claims (7)
1. The disinfectant is characterized by specifically comprising the following raw materials in parts by weight:
2. the disinfectant as set forth in claim 1, wherein said pH regulator is at least one of hydrochloric acid and phosphoric acid in an amount of 0.1 mol/L.
3. The disinfectant liquid according to claim 1, wherein the aloe extract is aloe vera, and the concentration of the aloe extract is 1 g/ml. Mixing fresh aloe and water at a mass ratio of 1:8, pulverizing, squeezing, filtering, and concentrating to obtain 1g/mL solution.
4. The disinfecting solution of claim 1, wherein the purified water is prepared from drinking water through a multi-media filter, an activated carbon filter, a softener and a two-stage reverse osmosis device in sequence.
5. The method for preparing the disinfectant liquid according to claim 1, comprising the steps of:
the method comprises the following steps: adding purified water into a vacuum emulsification stirrer, adding sodium chloride from a hopper of the vacuum emulsification stirrer, vacuumizing and stirring;
step two: adding sodium hypochlorite into a vacuum emulsification stirrer from a hopper, vacuumizing and stirring;
step three: slowly adding a pH regulator and glycyrrhizic acid from a hopper, stirring while adding, regulating the pH value to 3.0-6.5, vacuumizing and continuously stirring to react to generate hypochlorous acid;
step four: slowly adding Aloe extract from hopper, and stirring.
6. The method of claim 5, wherein the vacuum emulsification mixer in the first and second steps has a mixing speed of 20Hz and a mixing time of 5 min; in the third step, the stirring speed is 20HZ, and the stirring time is 10 min.
7. The use of the sanitizing liquid prepared by the method of claim 5 for cleaning vegetables, fruits and tableware.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110454922.XA CN113170801A (en) | 2021-04-26 | 2021-04-26 | Disinfectant and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110454922.XA CN113170801A (en) | 2021-04-26 | 2021-04-26 | Disinfectant and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113170801A true CN113170801A (en) | 2021-07-27 |
Family
ID=76926172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110454922.XA Pending CN113170801A (en) | 2021-04-26 | 2021-04-26 | Disinfectant and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113170801A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113647417A (en) * | 2021-08-18 | 2021-11-16 | 江苏耀研达科技发展有限公司 | Ecological environment-friendly plant-derived virus and bacterium inactivator, preparation method, inactivation identification method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472589A (en) * | 2014-11-26 | 2015-04-01 | 成都顺发消洗科技有限公司 | Disinfecting liquid for fruits and vegetables and preparation method thereof |
| CN110200989A (en) * | 2019-06-14 | 2019-09-06 | 广州泰道安医疗科技有限公司 | A kind of stable antiseptic solution for clinic |
| CN111990410A (en) * | 2020-09-03 | 2020-11-27 | 感控卫士(山东)医疗科技有限公司 | Hypochlorous acid disinfectant, preparation method thereof and preparation method of disinfectant wet tissue |
-
2021
- 2021-04-26 CN CN202110454922.XA patent/CN113170801A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472589A (en) * | 2014-11-26 | 2015-04-01 | 成都顺发消洗科技有限公司 | Disinfecting liquid for fruits and vegetables and preparation method thereof |
| CN110200989A (en) * | 2019-06-14 | 2019-09-06 | 广州泰道安医疗科技有限公司 | A kind of stable antiseptic solution for clinic |
| CN111990410A (en) * | 2020-09-03 | 2020-11-27 | 感控卫士(山东)医疗科技有限公司 | Hypochlorous acid disinfectant, preparation method thereof and preparation method of disinfectant wet tissue |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113647417A (en) * | 2021-08-18 | 2021-11-16 | 江苏耀研达科技发展有限公司 | Ecological environment-friendly plant-derived virus and bacterium inactivator, preparation method, inactivation identification method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Allende et al. | Impact of wash water quality on sensory and microbial quality, including Escherichia coli cross-contamination, of fresh-cut escarole | |
| JP6803896B2 (en) | Antimicrobial agent containing hypochlorous acid | |
| AU771148B2 (en) | Adduct having an acidic solution of sparingly-soluble group IIA complexes | |
| KR101279870B1 (en) | A processing method of liquid by using salmon testis extract for anti-aging and anti-oxidant care | |
| Cai et al. | Response of formed‐biofilm of Enterobacter cloacae, Klebsiella oxytoca, and Citrobacter freundii to chlorite‐based disinfectants | |
| CN112741087A (en) | Cold chain food disinfectant and preparation method thereof | |
| KR20190115372A (en) | Method and apparatus for producing of pure chlorite solution | |
| CN113170801A (en) | Disinfectant and preparation method and application thereof | |
| CN113142240A (en) | Environment disinfectant and preparation method thereof | |
| US6331514B1 (en) | Sterilizing and disinfecting compound | |
| CN112120042A (en) | Hypochlorous acid disinfectant with efficient sterilization function and preparation method thereof | |
| CN113197199B (en) | Instrument disinfectant, and preparation method and application thereof | |
| CN101646340B (en) | Production of a viable, storable worm egg suspension | |
| KR20210082627A (en) | Method and apparatus for producing of pure chlorite solution | |
| CN101548683B (en) | Liquid disinfectant and method of producing the same | |
| CN112868665A (en) | Disinfectant containing supermolecule silver and preparation method thereof | |
| CN112972281A (en) | Disinfectant-grade hand sanitizer containing salicylic acid microcapsules and preparation method thereof | |
| CN110721326A (en) | Manufacturing and application of multifunctional laboratory disinfection cleaning machine | |
| CN113209212A (en) | Hand disinfectant and preparation method thereof | |
| CN114208845B (en) | Hypochlorous acid disinfectant and preparation method and application thereof | |
| CN114916543B (en) | Disinfection stock solution and preparation method and application thereof | |
| Abreu et al. | The efficacy and stability of five sanitizing agents challenged with reference microorganisms and clean area isolates | |
| CN113180056B (en) | Hypochlorous acid disinfectant | |
| KR20030085436A (en) | Septic Lintless Wiper | |
| Akhtar et al. | Continental Veterinary Journal |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210727 |
|
| RJ01 | Rejection of invention patent application after publication |